AlfalfaGEDB | MsG0780039523.01

M. sativa cultivar ZhongmuNo.1 / MsG0780039523.01

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0780039523.01.T01	AES79950.1	87.619	105	3	1	1	105	1	95	2.47E-55	177
MsG0780039523.01.T02	AES79950.1	98.361	61	1	0	1	61	35	95	1.03E-31	115
MsG0780039523.01.T03	AES79950.1	97.778	45	1	0	1	45	51	95	3.79E-20	85.5

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0780039523.01.T01	G7L0A6	87.619	105	3	1	1	105	1	95	1.18e-55	177
MsG0780039523.01.T02	G7L0A6	98.361	61	1	0	1	61	35	95	4.92e-32	115
MsG0780039523.01.T03	G7L0A6	97.778	45	1	0	1	45	51	95	1.81e-20	85.5

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0180000599.01	MsG0780039523.01	0.807197	5.685137e-50	3.146769e-47
MsG0480018670.01	MsG0780039523.01	0.807114	5.920390e-50	3.269769e-47
MsG0480018676.01	MsG0780039523.01	0.808771	2.628733e-50	1.516105e-47
MsG0480020229.01	MsG0780039523.01	0.811090	8.330175e-51	5.109402e-48
MsG0480021248.01	MsG0780039523.01	0.803665	3.128608e-49	1.581319e-46
MsG0580024340.01	MsG0780039523.01	0.825198	5.343101e-54	4.837244e-51
MsG0580024759.01	MsG0780039523.01	0.802029	6.810202e-49	3.301359e-46
MsG0280009954.01	MsG0780039523.01	0.805291	1.432827e-49	7.551792e-47
MsG0280009960.01	MsG0780039523.01	0.800118	1.674759e-48	7.734830e-46
MsG0780036509.01	MsG0780039523.01	0.803735	3.024801e-49	1.531713e-46
MsG0780039523.01	MsG0880042044.01	0.801309	9.567192e-49	4.554149e-46
MsG0780039523.01	MsG0880042086.01	0.808824	2.561625e-50	1.479510e-47
MsG0780039523.01	MsG0880044487.01	0.801356	9.361194e-49	4.461329e-46
MsG0780039523.01	MsG0880044620.01	0.810478	1.129935e-50	6.818281e-48
MsG0780039523.01	MsG0880045402.01	0.817541	3.125673e-52	2.282760e-49
MsG0780039523.01	MsG0880046200.01	0.843235	1.586953e-58	2.466996e-55

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0780039523.01.T01	MTR_7g075120	87.619	105	3	1	1	105	1	95	3.00e-59	177
MsG0780039523.01.T02	MTR_7g075120	98.361	61	1	0	1	61	35	95	1.25e-35	115
MsG0780039523.01.T03	MTR_7g075120	97.778	45	1	0	1	45	51	95	4.59e-24	85.5

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0780039523.01.T01	AT3G60890	47.458	59	31	0	38	96	40	98	1.06e-12	59.7

Find 28 sgRNAs with CRISPR-Local

Find 35 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AACAAGGAAAGTTAAGATTC+AGG	0.334027	7:-65246761	None:intergenic
CAAGCTATTAATTAAATTCA+TGG	0.344700	7:+65247020	MsG0780039523.01.T01:three_prime_UTR
GTGATATAATAAAGATGAAT+AGG	0.370948	7:+65246830	MsG0780039523.01.T01:three_prime_UTR
TGACATCTCAAAGGTATGTT+TGG	0.371484	7:+65246924	MsG0780039523.01.T01:three_prime_UTR
CATGATATTGTGAGATATAT+TGG	0.382496	7:+65246883	MsG0780039523.01.T01:three_prime_UTR
TTTGTTGTGAAAGTTTCATT+TGG	0.391141	7:-65246629	None:intergenic
CATGAATTTAATTAATAGCT+TGG	0.405233	7:-65247019	None:intergenic
TTCTATGTAATGGTTACAAT+TGG	0.414110	7:-65246860	None:intergenic
TGTGAGATATATTGGTGTAT+TGG	0.421056	7:+65246891	MsG0780039523.01.T01:three_prime_UTR
CTTCTCATTCTGCTCTATGA+TGG	0.425083	7:-65246550	None:intergenic
AAATAATAGCTAGTTATTGT+TGG	0.453707	7:-65246661	None:intergenic
CAATATCATGTTCTATGTAA+TGG	0.456616	7:-65246870	None:intergenic
TCATTTGGAGCTGAGACATA+AGG	0.458680	7:-65246614	None:intergenic
ACAAGGAAAGTTAAGATTCA+GGG	0.464418	7:-65246760	None:intergenic
GTGCCTACAGTTGTGACTCT+TGG	0.467590	7:-65246326	None:intergenic
TTAGGTTGAGCCACCTCAAA+AGG	0.471420	7:+65246350	MsG0780039523.01.T01:CDS
GTCACAACTGTAGGCACCTT+AGG	0.478643	7:+65246332	MsG0780039523.01.T01:CDS
CTCAAAGGTATGTTTGGAGA+AGG	0.496017	7:+65246930	MsG0780039523.01.T01:three_prime_UTR
ATTCTCTTTGTGAAGAAGCA+TGG	0.505800	7:-65246586	None:intergenic
GAATAGAGAACAAAATGTCT+TGG	0.513346	7:+65246960	MsG0780039523.01.T01:three_prime_UTR
AAGCTATTAATTAAATTCAT+GGG	0.521024	7:+65247021	MsG0780039523.01.T01:three_prime_UTR
ATTGGTGATGCAGAAGGAAA+AGG	0.539434	7:+65246466	MsG0780039523.01.T01:intron
CAGTTGTGACTCTTGGAAAG+AGG	0.571906	7:-65246319	None:intergenic
TCAAAGGTATGTTTGGAGAA+GGG	0.574900	7:+65246931	MsG0780039523.01.T01:three_prime_UTR
TCAAATGTTTGACATCTCAA+AGG	0.581462	7:+65246915	MsG0780039523.01.T01:three_prime_UTR
CAGAAGGAAAAGGATGATTG+AGG	0.594182	7:+65246476	MsG0780039523.01.T01:intron
TTTCCAAGAGTCACAACTGT+AGG	0.599658	7:+65246323	MsG0780039523.01.T01:CDS
CAAGGAAAGTTAAGATTCAG+GGG	0.620292	7:-65246759	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AAGCTATTAATTAAATTCAT+GGG	+	Chr7:65247021-65247040	MsG0780039523.01.T01:three_prime_UTR	15.0%
!!	TTAAAAAACTAAAACAAACA+AGG	-	Chr7:65246780-65246799	None:intergenic	15.0%
!!	AAATAATAGCTAGTTATTGT+TGG	-	Chr7:65246664-65246683	None:intergenic	20.0%
!!	CAAGCTATTAATTAAATTCA+TGG	+	Chr7:65247020-65247039	MsG0780039523.01.T01:three_prime_UTR	20.0%
!!	CATGAATTTAATTAATAGCT+TGG	-	Chr7:65247022-65247041	None:intergenic	20.0%
!!	GAAAAACTTGAAATTGTATA+TGG	+	Chr7:65246518-65246537	MsG0780039523.01.T01:CDS	20.0%
!!	GTGATATAATAAAGATGAAT+AGG	+	Chr7:65246830-65246849	MsG0780039523.01.T01:three_prime_UTR	20.0%
!	CAATATCATGTTCTATGTAA+TGG	-	Chr7:65246873-65246892	None:intergenic	25.0%
!	CATGATATTGTGAGATATAT+TGG	+	Chr7:65246883-65246902	MsG0780039523.01.T01:three_prime_UTR	25.0%
!	TTCTATGTAATGGTTACAAT+TGG	-	Chr7:65246863-65246882	None:intergenic	25.0%
!!	GATTAACTAACTTGTATGAT+TGG	+	Chr7:65246448-65246467	MsG0780039523.01.T01:intron	25.0%
!!	TATTAGAAGAATACCTTTTG+AGG	-	Chr7:65246366-65246385	None:intergenic	25.0%
!!	TTTGTTGTGAAAGTTTCATT+TGG	-	Chr7:65246632-65246651	None:intergenic	25.0%
	AACAAGGAAAGTTAAGATTC+AGG	-	Chr7:65246764-65246783	None:intergenic	30.0%
	ACAAGGAAAGTTAAGATTCA+GGG	-	Chr7:65246763-65246782	None:intergenic	30.0%
	GAATAGAGAACAAAATGTCT+TGG	+	Chr7:65246960-65246979	MsG0780039523.01.T01:three_prime_UTR	30.0%
	TCAAATGTTTGACATCTCAA+AGG	+	Chr7:65246915-65246934	MsG0780039523.01.T01:three_prime_UTR	30.0%
	TGTGAGATATATTGGTGTAT+TGG	+	Chr7:65246891-65246910	MsG0780039523.01.T01:three_prime_UTR	30.0%
	ATTCTCTTTGTGAAGAAGCA+TGG	-	Chr7:65246589-65246608	None:intergenic	35.0%
	CAAGGAAAGTTAAGATTCAG+GGG	-	Chr7:65246762-65246781	None:intergenic	35.0%
!	TCAAAGGTATGTTTGGAGAA+GGG	+	Chr7:65246931-65246950	MsG0780039523.01.T01:three_prime_UTR	35.0%
!	TGACATCTCAAAGGTATGTT+TGG	+	Chr7:65246924-65246943	MsG0780039523.01.T01:three_prime_UTR	35.0%
!!	TAGAAGAATACCTTTTGAGG+TGG	-	Chr7:65246363-65246382	None:intergenic	35.0%
	CAGAAGGAAAAGGATGATTG+AGG	+	Chr7:65246476-65246495	MsG0780039523.01.T01:intron	40.0%
	CTTCTCATTCTGCTCTATGA+TGG	-	Chr7:65246553-65246572	None:intergenic	40.0%
	TTTCCAAGAGTCACAACTGT+AGG	+	Chr7:65246323-65246342	MsG0780039523.01.T01:CDS	40.0%
!	CTCAAAGGTATGTTTGGAGA+AGG	+	Chr7:65246930-65246949	MsG0780039523.01.T01:three_prime_UTR	40.0%
!	TCATTTGGAGCTGAGACATA+AGG	-	Chr7:65246617-65246636	None:intergenic	40.0%
!!	ATTGGTGATGCAGAAGGAAA+AGG	+	Chr7:65246466-65246485	MsG0780039523.01.T01:intron	40.0%
!!	TGTATGATTGGTGATGCAGA+AGG	+	Chr7:65246460-65246479	MsG0780039523.01.T01:intron	40.0%
	CAGTTGTGACTCTTGGAAAG+AGG	-	Chr7:65246322-65246341	None:intergenic	45.0%
!!	TTAGGTTGAGCCACCTCAAA+AGG	+	Chr7:65246350-65246369	MsG0780039523.01.T01:CDS	45.0%
!!!	TTTTGAGGTGGCTCAACCTA+AGG	-	Chr7:65246351-65246370	None:intergenic	45.0%
	GTCACAACTGTAGGCACCTT+AGG	+	Chr7:65246332-65246351	MsG0780039523.01.T01:CDS	50.0%
	GTGCCTACAGTTGTGACTCT+TGG	-	Chr7:65246329-65246348	None:intergenic	50.0%

Chromosome	Type	Strat	End	Strand	Name
Chr7	gene	65246280	65247054	65246280	ID=MsG0780039523.01;Name=MsG0780039523.01
Chr7	mRNA	65246280	65247054	65246280	ID=MsG0780039523.01.T01;Parent=MsG0780039523.01;Name=MsG0780039523.01.T01;_AED=0.38;_eAED=0.38;_QI=0\|0\|0.5\|1\|0\|0\|2\|380\|105
Chr7	exon	65246280	65246401	65246280	ID=MsG0780039523.01.T01:exon:10208;Parent=MsG0780039523.01.T01
Chr7	exon	65246479	65247054	65246479	ID=MsG0780039523.01.T01:exon:10209;Parent=MsG0780039523.01.T01
Chr7	CDS	65246280	65246401	65246280	ID=MsG0780039523.01.T01:cds;Parent=MsG0780039523.01.T01
Chr7	CDS	65246479	65246674	65246479	ID=MsG0780039523.01.T01:cds;Parent=MsG0780039523.01.T01
Chr7	three_prime_UTR	65246675	65247054	65246675	ID=MsG0780039523.01.T01:three_prime_utr;Parent=MsG0780039523.01.T01
Chr7	mRNA	65246489	65246674	65246489	ID=MsG0780039523.01.T02;Parent=MsG0780039523.01;Name=MsG0780039523.01.T02;_AED=0.47;_eAED=0.48;_QI=0\|-1\|0\|1\|-1\|0\|1\|0\|61
Chr7	exon	65246489	65246674	65246489	ID=MsG0780039523.01.T02:exon:10210;Parent=MsG0780039523.01.T02
Chr7	CDS	65246489	65246674	65246489	ID=MsG0780039523.01.T02:cds;Parent=MsG0780039523.01.T02
Chr7	mRNA	65246537	65246674	65246537	ID=MsG0780039523.01.T03;Parent=MsG0780039523.01;Name=MsG0780039523.01.T03;_AED=0.48;_eAED=0.49;_QI=0\|-1\|0\|1\|-1\|0\|1\|0\|45
Chr7	exon	65246537	65246674	65246537	ID=MsG0780039523.01.T03:exon:10211;Parent=MsG0780039523.01.T03
Chr7	CDS	65246537	65246674	65246537	ID=MsG0780039523.01.T03:cds;Parent=MsG0780039523.01.T03

Gene Sequence

>MsG0780039523.01.T01

ATGTGTTGTTTCTCTTCACAACATGACACTCTTTATGCTCCTCTTTCCAAGAGTCACAACTGTAGGCACCTTAGGTTGAGCCACCTCAAAAGGTATTCTTCTAATATTTTTTTATCATATAGAAGGAAAAGGATGATTGAGGTGAAAGCACAGATTAAGATGAAAAACTTGAAATTGTATATGGAGAATCAAACCATCATAGAGCAGAATGAGAAGTTGAGAAAACAAGCCATGCTTCTTCACAAAGAGAATCAAGACCTTATGTCTCAGCTCCAAATGAAACTTTCACAACAAAATATCAATACCAACAATAACTAG

>MsG0780039523.01.T02

ATGATTGAGGTGAAAGCACAGATTAAGATGAAAAACTTGAAATTGTATATGGAGAATCAAACCATCATAGAGCAGAATGAGAAGTTGAGAAAACAAGCCATGCTTCTTCACAAAGAGAATCAAGACCTTATGTCTCAGCTCCAAATGAAACTTTCACAACAAAATATCAATACCAACAATAACTAG

>MsG0780039523.01.T03

ATGGAGAATCAAACCATCATAGAGCAGAATGAGAAGTTGAGAAAACAAGCCATGCTTCTTCACAAAGAGAATCAAGACCTTATGTCTCAGCTCCAAATGAAACTTTCACAACAAAATATCAATACCAACAATAACTAG

Protein sequence

>MsG0780039523.01.T01

MCCFSSQHDTLYAPLSKSHNCRHLRLSHLKRYSSNIFLSYRRKRMIEVKAQIKMKNLKLYMENQTIIEQNEKLRKQAMLLHKENQDLMSQLQMKLSQQNINTNNN*

>MsG0780039523.01.T02

MIEVKAQIKMKNLKLYMENQTIIEQNEKLRKQAMLLHKENQDLMSQLQMKLSQQNINTNNN*

>MsG0780039523.01.T03

MENQTIIEQNEKLRKQAMLLHKENQDLMSQLQMKLSQQNINTNNN*