Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780039706.01.T01 | ABN08855.1 | 90.698 | 215 | 19 | 1 | 2 | 215 | 18 | 232 | 1.02E-134 | 388 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780039706.01.T01 | O82775 | 38.009 | 221 | 92 | 7 | 2 | 219 | 15 | 193 | 8.93E-31 | 116 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780039706.01.T01 | A2Q5F5 | 90.698 | 215 | 19 | 1 | 2 | 215 | 18 | 232 | 4.85e-135 | 388 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0780039595.01 | MsG0780039706.01 | 0.853768 | 1.920513e-61 | 4.195321e-58 |
PPI
| Gene1 | Gene2 | Type |
|---|---|---|
| MsG0780039706.01 | MsG0280011191.01 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780039706.01.T01 | MTR_2g027720 | 90.698 | 215 | 19 | 1 | 2 | 215 | 18 | 232 | 1.23e-138 | 388 |
| MsG0780039706.01.T01 | MTR_7g077860 | 83.333 | 240 | 19 | 1 | 2 | 220 | 18 | 257 | 2.63e-138 | 390 |
| MsG0780039706.01.T01 | MTR_7g077860 | 83.333 | 240 | 19 | 1 | 2 | 220 | 18 | 257 | 3.70e-138 | 388 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780039706.01.T01 | AT2G45980 | 38.009 | 221 | 92 | 7 | 2 | 219 | 15 | 193 | 9.11e-32 | 116 |
| MsG0780039706.01.T01 | AT4G00355 | 40.789 | 228 | 88 | 9 | 2 | 220 | 15 | 204 | 5.50e-30 | 112 |
| MsG0780039706.01.T01 | AT4G00355 | 40.789 | 228 | 88 | 9 | 2 | 220 | 15 | 204 | 5.50e-30 | 112 |
| MsG0780039706.01.T01 | AT4G00355 | 40.789 | 228 | 88 | 9 | 2 | 220 | 15 | 204 | 5.50e-30 | 112 |
| MsG0780039706.01.T01 | AT4G00355 | 40.789 | 228 | 88 | 9 | 2 | 220 | 15 | 204 | 1.07e-29 | 111 |
Find 43 sgRNAs with CRISPR-Local
Find 54 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGTGAGATTCAGAATGAATT+TGG | 0.214211 | 7:-67842570 | MsG0780039706.01.T01:CDS |
| GGCACAACTCTACCAGATTT+TGG | 0.228578 | 7:-67842377 | MsG0780039706.01.T01:CDS |
| GAAGAAAACTTGACATTAGA+AGG | 0.322875 | 7:-67842486 | MsG0780039706.01.T01:CDS |
| CTCCTTTGCATGAGCATAGA+TGG | 0.333088 | 7:+67842106 | None:intergenic |
| CAGATCTTCCTTGCGGAGCT+TGG | 0.354733 | 7:-67842148 | MsG0780039706.01.T01:CDS |
| TGTCTCCTGCTCTGCTGATT+CGG | 0.382713 | 7:+67842235 | None:intergenic |
| AGCAGCAATGTCTGTTTCAC+TGG | 0.393072 | 7:+67842298 | None:intergenic |
| CAAAGGAGGCAAATACATTA+TGG | 0.393151 | 7:-67842091 | MsG0780039706.01.T01:CDS |
| TTCATCCTTTCCACTAGGCT+TGG | 0.405829 | 7:+67842506 | None:intergenic |
| AAACTTGACATTAGAAGGAT+TGG | 0.407672 | 7:-67842481 | MsG0780039706.01.T01:CDS |
| ATCTTCCTTGCGGAGCTTGG+TGG | 0.419362 | 7:-67842145 | MsG0780039706.01.T01:CDS |
| TTTGACAGTGATAAATCATC+AGG | 0.420814 | 7:+67842203 | None:intergenic |
| TTGCAGCAACAGTGATGGTT+TGG | 0.461680 | 7:-67842058 | MsG0780039706.01.T01:CDS |
| TCAACTTATGCAGCTGCTCC+TGG | 0.470344 | 7:-67842738 | MsG0780039706.01.T01:CDS |
| GAAGTGACCAGACATGAATA+AGG | 0.494471 | 7:+67842641 | None:intergenic |
| AACTCAACTTCACGTGGACC+AGG | 0.507089 | 7:+67842720 | None:intergenic |
| TTCATGCTTACTTGGTGGAA+AGG | 0.529697 | 7:+67842617 | None:intergenic |
| TCAAACGCCTTATTCATGTC+TGG | 0.529833 | 7:-67842648 | MsG0780039706.01.T01:CDS |
| AACAACCGAATCAGCAGAGC+AGG | 0.534322 | 7:-67842240 | MsG0780039706.01.T01:CDS |
| TAAGCATGAAAACTTGCCAT+TGG | 0.534678 | 7:-67842604 | MsG0780039706.01.T01:CDS |
| ATCAGCAGAGCAGGAGACAA+AGG | 0.541008 | 7:-67842231 | MsG0780039706.01.T01:CDS |
| CAAAGATATCGCCTCTGTAG+AGG | 0.545508 | 7:-67842544 | MsG0780039706.01.T01:CDS |
| CGCTTCCACCAAGCTCCGCA+AGG | 0.554434 | 7:+67842140 | None:intergenic |
| CATGGAAAACAGTTTGAGGA+AGG | 0.557886 | 7:-67842398 | MsG0780039706.01.T01:intron |
| GCTCCGCAAGGAAGATCTGA+AGG | 0.560737 | 7:+67842152 | None:intergenic |
| TCCACGTGAAGTTGAGTTGA+AGG | 0.562439 | 7:-67842715 | MsG0780039706.01.T01:CDS |
| TGCGGAGCTTGGTGGAAGCG+AGG | 0.567411 | 7:-67842137 | MsG0780039706.01.T01:CDS |
| TTTCATTGCAGCAACAGTGA+TGG | 0.573380 | 7:-67842063 | MsG0780039706.01.T01:CDS |
| CATCTATGCTCATGCAAAGG+AGG | 0.578296 | 7:-67842105 | MsG0780039706.01.T01:CDS |
| GCAGCAATGTCTGTTTCACT+GGG | 0.581872 | 7:+67842299 | None:intergenic |
| TGAGTTGAAGGATGATGACA+AGG | 0.594626 | 7:-67842703 | MsG0780039706.01.T01:CDS |
| TTAGAAGGATTGGATGTCAA+AGG | 0.596759 | 7:-67842471 | MsG0780039706.01.T01:CDS |
| AGCGACCAAGCCTAGTGGAA+AGG | 0.597200 | 7:-67842511 | MsG0780039706.01.T01:CDS |
| TCCTTCAACTCAACTTCACG+TGG | 0.602249 | 7:+67842714 | None:intergenic |
| CTCCATCTATGCTCATGCAA+AGG | 0.603944 | 7:-67842108 | MsG0780039706.01.T01:CDS |
| TCACTATAGTCAGTTTCCAA+TGG | 0.627762 | 7:+67842588 | None:intergenic |
| AACCCTTCAGATCTTCCTTG+CGG | 0.630132 | 7:-67842155 | MsG0780039706.01.T01:CDS |
| GAAGAAGCGACCAAGCCTAG+TGG | 0.639637 | 7:-67842516 | MsG0780039706.01.T01:CDS |
| CTCCGCAAGGAAGATCTGAA+GGG | 0.679694 | 7:+67842153 | None:intergenic |
| CTTTGCATGAGCATAGATGG+AGG | 0.694461 | 7:+67842109 | None:intergenic |
| TTGAAGGATGATGACAAGGA+AGG | 0.695939 | 7:-67842699 | MsG0780039706.01.T01:CDS |
| TTCTTCACGAGCCTCTACAG+AGG | 0.708502 | 7:+67842533 | None:intergenic |
| CAGCAATGTCTGTTTCACTG+GGG | 0.782536 | 7:+67842300 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | ATCAACAGATTATCTATTCA+TGG | - | Chr7:67842401-67842420 | MsG0780039706.01.T01:intron | 25.0% |
| AAACTTGACATTAGAAGGAT+TGG | - | Chr7:67842336-67842355 | MsG0780039706.01.T01:CDS | 30.0% | |
| AGTGAGATTCAGAATGAATT+TGG | - | Chr7:67842247-67842266 | MsG0780039706.01.T01:CDS | 30.0% | |
| GAAGAAAACTTGACATTAGA+AGG | - | Chr7:67842331-67842350 | MsG0780039706.01.T01:CDS | 30.0% | |
| TATTCATGGAAAACAGTTTG+AGG | - | Chr7:67842415-67842434 | MsG0780039706.01.T01:intron | 30.0% | |
| TTTGACAGTGATAAATCATC+AGG | + | Chr7:67842617-67842636 | None:intergenic | 30.0% | |
| ! | GGAAAGAATTGTATTTTGCA+GGG | + | Chr7:67842499-67842518 | None:intergenic | 30.0% |
| ! | TTTTCAGTCAAACCAAAATC+TGG | + | Chr7:67842455-67842474 | None:intergenic | 30.0% |
| !!! | TTTTGGTTTGACTGAAAAAG+AGG | - | Chr7:67842457-67842476 | MsG0780039706.01.T01:intron | 30.0% |
| CAAAGGAGGCAAATACATTA+TGG | - | Chr7:67842726-67842745 | MsG0780039706.01.T01:CDS | 35.0% | |
| TAAGCATGAAAACTTGCCAT+TGG | - | Chr7:67842213-67842232 | MsG0780039706.01.T01:CDS | 35.0% | |
| TCACTATAGTCAGTTTCCAA+TGG | + | Chr7:67842232-67842251 | None:intergenic | 35.0% | |
| TTAGAAGGATTGGATGTCAA+AGG | - | Chr7:67842346-67842365 | MsG0780039706.01.T01:CDS | 35.0% | |
| ! | AAGTTTTCATGCTTACTTGG+TGG | + | Chr7:67842208-67842227 | None:intergenic | 35.0% |
| ! | GGGAAAGAATTGTATTTTGC+AGG | + | Chr7:67842500-67842519 | None:intergenic | 35.0% |
| ! | TTTTCTTCATCCTTTCCACT+AGG | + | Chr7:67842319-67842338 | None:intergenic | 35.0% |
| !!! | AGGGTTTTGTTTGTCATCTT+TGG | + | Chr7:67842648-67842667 | None:intergenic | 35.0% |
| CATGGAAAACAGTTTGAGGA+AGG | - | Chr7:67842419-67842438 | MsG0780039706.01.T01:intron | 40.0% | |
| GAAGTGACCAGACATGAATA+AGG | + | Chr7:67842179-67842198 | None:intergenic | 40.0% | |
| TTGAAGGATGATGACAAGGA+AGG | - | Chr7:67842118-67842137 | MsG0780039706.01.T01:CDS | 40.0% | |
| TTGGATGTCAAAGGAGTTTG+AGG | - | Chr7:67842355-67842374 | MsG0780039706.01.T01:CDS | 40.0% | |
| TTTCATTGCAGCAACAGTGA+TGG | - | Chr7:67842754-67842773 | MsG0780039706.01.T01:CDS | 40.0% | |
| ! | GGCAAGTTTTCATGCTTACT+TGG | + | Chr7:67842211-67842230 | None:intergenic | 40.0% |
| ! | TCAAACGCCTTATTCATGTC+TGG | - | Chr7:67842169-67842188 | MsG0780039706.01.T01:CDS | 40.0% |
| ! | TGAGTTGAAGGATGATGACA+AGG | - | Chr7:67842114-67842133 | MsG0780039706.01.T01:CDS | 40.0% |
| ! | TTCATGCTTACTTGGTGGAA+AGG | + | Chr7:67842203-67842222 | None:intergenic | 40.0% |
| AACCCTTCAGATCTTCCTTG+CGG | - | Chr7:67842662-67842681 | MsG0780039706.01.T01:CDS | 45.0% | |
| AGCAGCAATGTCTGTTTCAC+TGG | + | Chr7:67842522-67842541 | None:intergenic | 45.0% | |
| CAAAGATATCGCCTCTGTAG+AGG | - | Chr7:67842273-67842292 | MsG0780039706.01.T01:CDS | 45.0% | |
| CAGCAATGTCTGTTTCACTG+GGG | + | Chr7:67842520-67842539 | None:intergenic | 45.0% | |
| CATCTATGCTCATGCAAAGG+AGG | - | Chr7:67842712-67842731 | MsG0780039706.01.T01:CDS | 45.0% | |
| CTCCATCTATGCTCATGCAA+AGG | - | Chr7:67842709-67842728 | MsG0780039706.01.T01:CDS | 45.0% | |
| CTCCTTTGCATGAGCATAGA+TGG | + | Chr7:67842714-67842733 | None:intergenic | 45.0% | |
| CTTTGCATGAGCATAGATGG+AGG | + | Chr7:67842711-67842730 | None:intergenic | 45.0% | |
| GCAGCAATGTCTGTTTCACT+GGG | + | Chr7:67842521-67842540 | None:intergenic | 45.0% | |
| TCCTTCAACTCAACTTCACG+TGG | + | Chr7:67842106-67842125 | None:intergenic | 45.0% | |
| TTCATCCTTTCCACTAGGCT+TGG | + | Chr7:67842314-67842333 | None:intergenic | 45.0% | |
| TTGCAGCAACAGTGATGGTT+TGG | - | Chr7:67842759-67842778 | MsG0780039706.01.T01:CDS | 45.0% | |
| ! | GGCACAACTCTACCAGATTT+TGG | - | Chr7:67842440-67842459 | MsG0780039706.01.T01:intron | 45.0% |
| !! | TCCACGTGAAGTTGAGTTGA+AGG | - | Chr7:67842102-67842121 | MsG0780039706.01.T01:CDS | 45.0% |
| AACAACCGAATCAGCAGAGC+AGG | - | Chr7:67842577-67842596 | MsG0780039706.01.T01:CDS | 50.0% | |
| AACTCAACTTCACGTGGACC+AGG | + | Chr7:67842100-67842119 | None:intergenic | 50.0% | |
| ATCAGCAGAGCAGGAGACAA+AGG | - | Chr7:67842586-67842605 | MsG0780039706.01.T01:CDS | 50.0% | |
| CTCCGCAAGGAAGATCTGAA+GGG | + | Chr7:67842667-67842686 | None:intergenic | 50.0% | |
| TCAACTTATGCAGCTGCTCC+TGG | - | Chr7:67842079-67842098 | MsG0780039706.01.T01:CDS | 50.0% | |
| TTCTTCACGAGCCTCTACAG+AGG | + | Chr7:67842287-67842306 | None:intergenic | 50.0% | |
| ! | TGTCTCCTGCTCTGCTGATT+CGG | + | Chr7:67842585-67842604 | None:intergenic | 50.0% |
| AGCGACCAAGCCTAGTGGAA+AGG | - | Chr7:67842306-67842325 | MsG0780039706.01.T01:CDS | 55.0% | |
| ATCTTCCTTGCGGAGCTTGG+TGG | - | Chr7:67842672-67842691 | MsG0780039706.01.T01:CDS | 55.0% | |
| CAGATCTTCCTTGCGGAGCT+TGG | - | Chr7:67842669-67842688 | MsG0780039706.01.T01:CDS | 55.0% | |
| GAAGAAGCGACCAAGCCTAG+TGG | - | Chr7:67842301-67842320 | MsG0780039706.01.T01:CDS | 55.0% | |
| GCTCCGCAAGGAAGATCTGA+AGG | + | Chr7:67842668-67842687 | None:intergenic | 55.0% | |
| CGCTTCCACCAAGCTCCGCA+AGG | + | Chr7:67842680-67842699 | None:intergenic | 65.0% | |
| ! | TGCGGAGCTTGGTGGAAGCG+AGG | - | Chr7:67842680-67842699 | MsG0780039706.01.T01:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr7 | gene | 67842055 | 67842784 | 67842055 | ID=MsG0780039706.01;Name=MsG0780039706.01 |
| Chr7 | mRNA | 67842055 | 67842784 | 67842055 | ID=MsG0780039706.01.T01;Parent=MsG0780039706.01;Name=MsG0780039706.01.T01;_AED=0.38;_eAED=0.38;_QI=0|0|0|1|1|1|2|0|222 |
| Chr7 | exon | 67842470 | 67842784 | 67842470 | ID=MsG0780039706.01.T01:exon:11585;Parent=MsG0780039706.01.T01 |
| Chr7 | exon | 67842055 | 67842408 | 67842055 | ID=MsG0780039706.01.T01:exon:11584;Parent=MsG0780039706.01.T01 |
| Chr7 | CDS | 67842470 | 67842784 | 67842470 | ID=MsG0780039706.01.T01:cds;Parent=MsG0780039706.01.T01 |
| Chr7 | CDS | 67842055 | 67842408 | 67842055 | ID=MsG0780039706.01.T01:cds;Parent=MsG0780039706.01.T01 |
| Gene Sequence |
| Protein sequence |
