AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0280011191.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280011191.01.T01 MTR_7g077860 77.340 203 12 1 1 169 1 203 1.24e-101 296
MsG0280011191.01.T01 MTR_7g077860 77.340 203 12 1 1 169 1 203 3.93e-101 296
MsG0280011191.01.T01 MTR_2g027720 66.010 203 15 2 1 169 1 183 6.76e-78 234
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280011191.01.T01 AT4G00355 41.964 112 44 3 6 98 3 112 7.08e-17 77.4
MsG0280011191.01.T01 AT4G00355 41.964 112 44 3 6 98 3 112 9.95e-17 77.0
MsG0280011191.01.T01 AT4G00355 41.964 112 44 3 6 98 3 112 9.95e-17 77.0
MsG0280011191.01.T01 AT4G00355 41.964 112 44 3 6 98 3 112 9.95e-17 77.0
MsG0280011191.01.T01 AT2G45980 42.500 120 46 5 1 106 1 111 1.89e-12 65.1

Find 45 sgRNAs with CRISPR-Local

Find 57 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGCACAACTCTACCAGATTT+TGG 0.228578 2:-80791604 MsG0280011191.01.T01:CDS
GAAGAAAACTTGACATTAGA+AGG 0.322875 2:-80791715 MsG0280011191.01.T01:CDS
CTCCTTTGCATGAGCATAGA+TGG 0.333088 2:+80791334 None:intergenic
CAGATCTTCCTTGCGGAGCT+TGG 0.354733 2:-80791376 MsG0280011191.01.T01:CDS
ATCAACAGATTATCTATTCA+TGG 0.363088 2:-80791643 MsG0280011191.01.T01:CDS
TGTCTCCTGCTCTGCTGATT+CGG 0.382713 2:+80791462 None:intergenic
AGTCTTCTCGCGGTAATGAC+TGG 0.388623 2:-80792009 MsG0280011191.01.T01:CDS
AGCAGCAATGTCTGTTTCAC+TGG 0.393072 2:+80791525 None:intergenic
TTCATCCTTTCCACTAGGCT+TGG 0.405829 2:+80791735 None:intergenic
AAACTTGACATTAGAAGGAT+TGG 0.407672 2:-80791710 MsG0280011191.01.T01:CDS
ATCTTCCTTGCGGAGCTTGG+TGG 0.419362 2:-80791373 MsG0280011191.01.T01:CDS
TCAACTTATGCAGCTGCTCC+TGG 0.470344 2:-80791965 MsG0280011191.01.T01:CDS
GGATGTGTCAAAGGAGTTTG+AGG 0.473894 2:-80791689 MsG0280011191.01.T01:CDS
GAAGTGACCAGACATGAATA+AGG 0.494471 2:+80791868 None:intergenic
GTCTTCTCGCGGTAATGACT+GGG 0.502839 2:-80792008 MsG0280011191.01.T01:CDS
AACTCAACTTCACGTGGACC+AGG 0.507089 2:+80791947 None:intergenic
TTATGAAGACAAAAGATGGC+AGG 0.513000 2:-80792052 None:intergenic
TATTCATGGAAAACAGTTTG+AGG 0.519571 2:-80791629 MsG0280011191.01.T01:CDS
TCAAACGCCTTATTCATGTC+TGG 0.529833 2:-80791875 MsG0280011191.01.T01:intron
ATGGCAGGAACTGAAGATGA+AGG 0.531057 2:-80792037 MsG0280011191.01.T01:CDS
TTTCATTGCAGCAACAGTGA+TGG 0.533829 2:-80791291 None:intergenic
AACAACCGAATCAGCAGAGC+AGG 0.534322 2:-80791467 MsG0280011191.01.T01:CDS
AGAAGGATTGGATGTGTCAA+AGG 0.536048 2:-80791698 MsG0280011191.01.T01:CDS
ATCAGCAGAGCAGGAGACAA+AGG 0.541008 2:-80791458 MsG0280011191.01.T01:CDS
CGCTTCCACCAAGCTCCGCA+AGG 0.554434 2:+80791368 None:intergenic
CATGGAAAACAGTTTGAGGA+AGG 0.557886 2:-80791625 MsG0280011191.01.T01:CDS
GCTCCGCAAGGAAGATCTGA+AGG 0.560737 2:+80791380 None:intergenic
TCCACGTGAAGTTGAGTTGA+AGG 0.562439 2:-80791942 MsG0280011191.01.T01:CDS
TGCGGAGCTTGGTGGAAGCG+AGG 0.567411 2:-80791365 MsG0280011191.01.T01:CDS
CATCTATGCTCATGCAAAGG+AGG 0.578296 2:-80791333 MsG0280011191.01.T01:CDS
TGGCAGGAACTGAAGATGAA+GGG 0.579156 2:-80792036 MsG0280011191.01.T01:CDS
GCAGCAATGTCTGTTTCACT+GGG 0.581872 2:+80791526 None:intergenic
TGAGTTGAAGGATGATGACA+AGG 0.594626 2:-80791930 MsG0280011191.01.T01:CDS
AGCGACCAAGCCTAGTGGAA+AGG 0.597200 2:-80791740 MsG0280011191.01.T01:CDS
GGCAGGAACTGAAGATGAAG+GGG 0.599526 2:-80792035 MsG0280011191.01.T01:CDS
TCCTTCAACTCAACTTCACG+TGG 0.602249 2:+80791941 None:intergenic
CTCCATCTATGCTCATGCAA+AGG 0.603944 2:-80791336 MsG0280011191.01.T01:CDS
AACCCTTCAGATCTTCCTTG+CGG 0.630132 2:-80791383 MsG0280011191.01.T01:CDS
GAAGAAGCGACCAAGCCTAG+TGG 0.639637 2:-80791745 MsG0280011191.01.T01:CDS
TTCTCGCGGTAATGACTGGG+AGG 0.656632 2:-80792005 MsG0280011191.01.T01:CDS
CTCCGCAAGGAAGATCTGAA+GGG 0.679694 2:+80791381 None:intergenic
GAAGGGGAAAAGTCTTCTCG+CGG 0.683817 2:-80792019 MsG0280011191.01.T01:CDS
CTTTGCATGAGCATAGATGG+AGG 0.694461 2:+80791337 None:intergenic
TTGAAGGATGATGACAAGGA+AGG 0.695939 2:-80791926 MsG0280011191.01.T01:CDS
CAGCAATGTCTGTTTCACTG+GGG 0.782536 2:+80791527 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! ATCAACAGATTATCTATTCA+TGG - Chr2:80791688-80791707 MsG0280011191.01.T01:CDS 25.0%
AAACTTGACATTAGAAGGAT+TGG - Chr2:80791621-80791640 MsG0280011191.01.T01:CDS 30.0%
AGTGAGATTCAGAATGAATT+TGG - Chr2:80791532-80791551 MsG0280011191.01.T01:CDS 30.0%
GAAGAAAACTTGACATTAGA+AGG - Chr2:80791616-80791635 MsG0280011191.01.T01:CDS 30.0%
TATTCATGGAAAACAGTTTG+AGG - Chr2:80791702-80791721 MsG0280011191.01.T01:CDS 30.0%
! GGAAAGAATTGTATTTTGCA+GGG + Chr2:80791786-80791805 None:intergenic 30.0%
! TTTTCAGTCAAACCAAAATC+TGG + Chr2:80791742-80791761 None:intergenic 30.0%
!!! TTTTGGTTTGACTGAAAAAG+AGG - Chr2:80791744-80791763 MsG0280011191.01.T01:CDS 30.0%
CAAAGGAGGCAAAAACATTA+TGG - Chr2:80792012-80792031 MsG0280011191.01.T01:CDS 35.0%
GTAAGCATGAAAATTGCCAT+TGG - Chr2:80791498-80791517 MsG0280011191.01.T01:CDS 35.0%
TCACTATAGTCAGTTTCCAA+TGG + Chr2:80791517-80791536 None:intergenic 35.0%
TTTCATGCTTACTTGTGGAA+AGG + Chr2:80791490-80791509 None:intergenic 35.0%
! GCAATTTTCATGCTTACTTG+TGG + Chr2:80791495-80791514 None:intergenic 35.0%
! GGGAAAGAATTGTATTTTGC+AGG + Chr2:80791787-80791806 None:intergenic 35.0%
! TTTTCTTCATCCTTTCCACT+AGG + Chr2:80791604-80791623 None:intergenic 35.0%
!!! AGGGTTTTGTTTGTCATCTT+TGG + Chr2:80791934-80791953 None:intergenic 35.0%
AGAAGGATTGGATGTGTCAA+AGG - Chr2:80791633-80791652 MsG0280011191.01.T01:CDS 40.0%
CATGGAAAACAGTTTGAGGA+AGG - Chr2:80791706-80791725 MsG0280011191.01.T01:CDS 40.0%
GAAGTGACCAGACATGAATA+AGG + Chr2:80791466-80791485 None:intergenic 40.0%
TTGAAGGATGATGACAAGGA+AGG - Chr2:80791405-80791424 MsG0280011191.01.T01:CDS 40.0%
! TCAAACGCCTTATTCATGTC+TGG - Chr2:80791456-80791475 MsG0280011191.01.T01:CDS 40.0%
! TGAGTTGAAGGATGATGACA+AGG - Chr2:80791401-80791420 MsG0280011191.01.T01:CDS 40.0%
AACCCTTCAGATCTTCCTTG+CGG - Chr2:80791948-80791967 MsG0280011191.01.T01:CDS 45.0%
AGCAGCAATGTCTGTTTCAC+TGG + Chr2:80791809-80791828 None:intergenic 45.0%
ATGGCAGGAACTGAAGATGA+AGG - Chr2:80791294-80791313 MsG0280011191.01.T01:CDS 45.0%
CAAAGATATCGCCTCTGTAG+AGG - Chr2:80791558-80791577 MsG0280011191.01.T01:CDS 45.0%
CAGCAATGTCTGTTTCACTG+GGG + Chr2:80791807-80791826 None:intergenic 45.0%
CATCTATGCTCATGCAAAGG+AGG - Chr2:80791998-80792017 MsG0280011191.01.T01:CDS 45.0%
CTCCATCTATGCTCATGCAA+AGG - Chr2:80791995-80792014 MsG0280011191.01.T01:CDS 45.0%
CTCCTTTGCATGAGCATAGA+TGG + Chr2:80792000-80792019 None:intergenic 45.0%
CTTTGCATGAGCATAGATGG+AGG + Chr2:80791997-80792016 None:intergenic 45.0%
GCAGCAATGTCTGTTTCACT+GGG + Chr2:80791808-80791827 None:intergenic 45.0%
GGATGTGTCAAAGGAGTTTG+AGG - Chr2:80791642-80791661 MsG0280011191.01.T01:CDS 45.0%
TCCTTCAACTCAACTTCACG+TGG + Chr2:80791393-80791412 None:intergenic 45.0%
TGGCAGGAACTGAAGATGAA+GGG - Chr2:80791295-80791314 MsG0280011191.01.T01:CDS 45.0%
TTCATCCTTTCCACTAGGCT+TGG + Chr2:80791599-80791618 None:intergenic 45.0%
! GGCACAACTCTACCAGATTT+TGG - Chr2:80791727-80791746 MsG0280011191.01.T01:CDS 45.0%
!! TCCACGTGAAGTTGAGTTGA+AGG - Chr2:80791389-80791408 MsG0280011191.01.T01:CDS 45.0%
AACAACCGAATCAGCAGAGC+AGG - Chr2:80791864-80791883 MsG0280011191.01.T01:intron 50.0%
AACTCAACTTCACGTGGACC+AGG + Chr2:80791387-80791406 None:intergenic 50.0%
AGTCTTCTCGCGGTAATGAC+TGG - Chr2:80791322-80791341 MsG0280011191.01.T01:CDS 50.0%
ATCAGCAGAGCAGGAGACAA+AGG - Chr2:80791873-80791892 MsG0280011191.01.T01:intron 50.0%
CTCCGCAAGGAAGATCTGAA+GGG + Chr2:80791953-80791972 None:intergenic 50.0%
GAAGGGGAAAAGTCTTCTCG+CGG - Chr2:80791312-80791331 MsG0280011191.01.T01:CDS 50.0%
GGCAGGAACTGAAGATGAAG+GGG - Chr2:80791296-80791315 MsG0280011191.01.T01:CDS 50.0%
GTCTTCTCGCGGTAATGACT+GGG - Chr2:80791323-80791342 MsG0280011191.01.T01:CDS 50.0%
TCAACTTATGCAGCTGCTCC+TGG - Chr2:80791366-80791385 MsG0280011191.01.T01:CDS 50.0%
TTCTTCACGAGCCTCTACAG+AGG + Chr2:80791572-80791591 None:intergenic 50.0%
! TGTCTCCTGCTCTGCTGATT+CGG + Chr2:80791872-80791891 None:intergenic 50.0%
AGCGACCAAGCCTAGTGGAA+AGG - Chr2:80791591-80791610 MsG0280011191.01.T01:CDS 55.0%
ATCTTCCTTGCGGAGCTTGG+TGG - Chr2:80791958-80791977 MsG0280011191.01.T01:CDS 55.0%
CAGATCTTCCTTGCGGAGCT+TGG - Chr2:80791955-80791974 MsG0280011191.01.T01:CDS 55.0%
GAAGAAGCGACCAAGCCTAG+TGG - Chr2:80791586-80791605 MsG0280011191.01.T01:CDS 55.0%
GCTCCGCAAGGAAGATCTGA+AGG + Chr2:80791954-80791973 None:intergenic 55.0%
TTCTCGCGGTAATGACTGGG+AGG - Chr2:80791326-80791345 MsG0280011191.01.T01:CDS 55.0%
CGCTTCCACCAAGCTCCGCA+AGG + Chr2:80791966-80791985 None:intergenic 65.0%
! TGCGGAGCTTGGTGGAAGCG+AGG - Chr2:80791966-80791985 MsG0280011191.01.T01:CDS 65.0%
Chromosome Type Strat End Strand Name
Chr2 gene 80791294 80792059 80791294 ID=MsG0280011191.01;Name=MsG0280011191.01
Chr2 mRNA 80791294 80792059 80791294 ID=MsG0280011191.01.T01;Parent=MsG0280011191.01;Name=MsG0280011191.01.T01;_AED=0.50;_eAED=0.53;_QI=0|0|0|1|1|1|2|0|221
Chr2 exon 80791876 80792059 80791876 ID=MsG0280011191.01.T01:exon:28709;Parent=MsG0280011191.01.T01
Chr2 exon 80791294 80791775 80791294 ID=MsG0280011191.01.T01:exon:28708;Parent=MsG0280011191.01.T01
Chr2 CDS 80791876 80792059 80791876 ID=MsG0280011191.01.T01:cds;Parent=MsG0280011191.01.T01
Chr2 CDS 80791294 80791775 80791294 ID=MsG0280011191.01.T01:cds;Parent=MsG0280011191.01.T01
Gene Sequence

>MsG0280011191.01.T01

ATGGCAGGAACTGAAGATGAAGGGGAAAAGTCTTCTCGCGGTAATGACTGGGAGGTTGTATCTCTCACAGCATCAACTTATGCAGCTGCTCCTGGTCCACGTGAAGTTGAGTTGAAGGATGATGACAAGGAAGGTGCATATGCAACAAATGAAGCTGAAACATCAAACGCCTTATTCATGTCTGAGGCTCGTGAAGAAGCGACCAAGCCTAGTGGAAAGGATGAAGAAAACTTGACATTAGAAGGATTGGATGTGTCAAAGGAGTTTGAGGATATGAAATATTATGATGAGAAAATCAACAGATTATCTATTCATGGAAAACAGTTTGAGGAAGGCACAACTCTACCAGATTTTGGTTTGACTGAAAAAGAGGAAAGTATATATCATAGCCCTGCAAAATACAATTCTTTCCCCAGTGAAACAGACATTGCTGCTGTGACAGTATACGATGAAAACGTAGTTGAGTCTGTAACAACCGAATCAGCAGAGCAGGAGACAAAGGTATCTCTGATGATTTATCACTGTCAAATAACTCTTCCAAAGATGACAAACAAAACCCTTCAGATCTTCCTTGCGGAGCTTGGTGGAAGCGAGGAGTTGCCTCCATCTATGCTCATGCAAAGGAGGCAAAAACATTATGGTCTGTTTTCATTGCAGCAACAGTGA

Protein sequence

>MsG0280011191.01.T01

MAGTEDEGEKSSRGNDWEVVSLTASTYAAAPGPREVELKDDDKEGAYATNEAETSNALFMSEAREEATKPSGKDEENLTLEGLDVSKEFEDMKYYDEKINRLSIHGKQFEEGTTLPDFGLTEKEESIYHSPAKYNSFPSETDIAAVTVYDENVVESVTTESAEQETKVSLMIYHCQITLPKMTNKTLQIFLAELGGSEELPPSMLMQRRQKHYGLFSLQQQ*