AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0780040157.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780040157.01.T01 MTR_7g085470 60.219 274 65 7 1 252 1 252 2.03e-95 283
MsG0780040157.01.T01 MTR_4g010050 56.429 280 85 5 1 252 1 271 5.31e-93 283
MsG0780040157.01.T01 MTR_2g104050 56.071 280 86 5 1 252 1 271 5.21e-88 269
MsG0780040157.01.T01 MTR_7g085570 52.814 231 66 7 17 217 1 218 1.72e-65 206
MsG0780040157.01.T01 MTR_7g104890 28.713 202 112 5 76 251 7 202 1.36e-18 84.7
MsG0780040157.01.T01 MTR_7g104890 25.283 265 165 6 13 251 78 335 2.85e-18 84.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780040157.01.T01 AT1G48900 52.500 280 96 5 1 252 1 271 2.68e-86 263
MsG0780040157.01.T01 AT1G48900 52.500 280 96 5 1 252 1 271 1.37e-85 263
MsG0780040157.01.T01 AT1G15310 50.357 280 102 5 1 252 1 271 2.30e-79 247
MsG0780040157.01.T01 AT5G49500 46.809 282 111 5 1 252 1 273 7.11e-70 223
MsG0780040157.01.T01 AT5G49500 69.565 115 30 1 138 252 42 151 1.51e-45 157
MsG0780040157.01.T01 AT5G03940 25.283 265 165 6 13 251 88 345 5.90e-17 80.5
MsG0780040157.01.T01 AT2G45770 31.148 183 90 4 100 251 163 340 1.32e-12 67.0

Find 100 sgRNAs with CRISPR-Local

Find 167 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGCTGTAATTCTTACTAAAA+TGG 0.204732 7:-73811075 MsG0780040157.01.T01:CDS
CGTTTGCTTCTTCTGAATAA+TGG 0.255113 7:-73810484 MsG0780040157.01.T01:three_prime_UTR
AAAATGGGTCTAGCGGATTT+TGG 0.261549 7:-73812603 MsG0780040157.01.T01:CDS
GCCTTTGTTAGTTGTCTATT+AGG 0.272733 7:-73810773 MsG0780040157.01.T01:three_prime_UTR
CCTTCTTTCGTTCCCAAATC+AGG 0.275500 7:-73812327 MsG0780040157.01.T01:CDS
TTACTCCATTCCGTCGGATT+AGG 0.276358 7:+73812665 None:intergenic
TTCTGATATTAATAATTATA+AGG 0.281499 7:+73810366 None:intergenic
CATCAATCAAGCAAGCAAAA+TGG 0.313620 7:-73812619 MsG0780040157.01.T01:exon
ATATCAGAAGTGTGTGAGTT+AGG 0.323806 7:-73810352 MsG0780040157.01.T01:three_prime_UTR
GAAACCAGATCTTGTTATAC+TGG 0.330957 7:-73811183 MsG0780040157.01.T01:CDS
ATTCAACAAGTTGTGTTTGA+TGG 0.337216 7:-73812375 MsG0780040157.01.T01:CDS
TACTCCATTCCGTCGGATTA+GGG 0.337412 7:+73812666 None:intergenic
GATTGATGAAGATGAAGATT+AGG 0.341229 7:+73812634 None:intergenic
ATTGATGAAGATGAAGATTA+GGG 0.344339 7:+73812635 None:intergenic
ACTCTGACTGAGACCTTCTC+AGG 0.360800 7:-73810622 MsG0780040157.01.T01:three_prime_UTR
CAAATGCCATGGCCTGCTTA+TGG 0.374424 7:+73811117 None:intergenic
TTAAAAGTTCCTATAGATAT+AGG 0.385054 7:+73810428 None:intergenic
GGTGAACATGGGTTCAATAT+AGG 0.395140 7:+73812476 None:intergenic
AGATATGAAGTTGCTAATAA+AGG 0.405971 7:+73810589 None:intergenic
CCTGATTTGGGAACGAAAGA+AGG 0.406262 7:+73812327 None:intergenic
GTGTGAGTTAGGGATGTAAA+TGG 0.406516 7:-73810341 MsG0780040157.01.T01:three_prime_UTR
ACAAGTGTTGTCATGTTTGT+TGG 0.414474 7:-73812300 MsG0780040157.01.T01:CDS
GCAAATTATTATCAGAAGAA+AGG 0.415429 7:-73812140 MsG0780040157.01.T01:CDS
CCAACTCTGTATAGCTATAG+AGG 0.415606 7:+73811433 None:intergenic
ATCAATCAAGCAAGCAAAAT+GGG 0.430214 7:-73812618 MsG0780040157.01.T01:exon
CAAAGAGTCCGGTCATTCAT+AGG 0.432678 7:-73810838 MsG0780040157.01.T01:CDS
AGCGTTGTTTCGGCGAGATT+AGG 0.433671 7:-73812515 MsG0780040157.01.T01:CDS
ACCTCTCATCCTATATCTAT+AGG 0.435557 7:-73810437 MsG0780040157.01.T01:three_prime_UTR
GTCATGTTTGTTGGTGCTCA+AGG 0.437604 7:-73812291 MsG0780040157.01.T01:intron
GTCTATTAGGCATGGATGAC+TGG 0.444236 7:-73810760 MsG0780040157.01.T01:three_prime_UTR
TATCAGAAGTGTGTGAGTTA+GGG 0.448759 7:-73810351 MsG0780040157.01.T01:three_prime_UTR
TGATGGACTCTGCAAATTGT+TGG 0.454884 7:-73812358 MsG0780040157.01.T01:CDS
GTCCGGTCATTCATAGGAAC+TGG 0.458419 7:-73810832 MsG0780040157.01.T01:CDS
TTGGGAACGAAAGAAGGGTT+AGG 0.473693 7:+73812333 None:intergenic
CAGAAGAAAGGATTAAAGAC+AGG 0.473847 7:-73812128 MsG0780040157.01.T01:intron
CTTCTTTCGTTCCCAAATCA+GGG 0.476991 7:-73812326 MsG0780040157.01.T01:CDS
TCATAGGAACTGGTGACAAA+TGG 0.477536 7:-73810822 MsG0780040157.01.T01:exon
ACGCATGTCTCGGGTGAACA+TGG 0.477955 7:+73812464 None:intergenic
TAAAGATGAGCACGGATAGT+AGG 0.478067 7:-73810317 MsG0780040157.01.T01:three_prime_UTR
TTTCCTATCCGGTGTCGGAT+CGG 0.478615 7:+73812739 None:intergenic
ACCTGCCAGAAAGAAAAGTC+TGG 0.479553 7:-73810691 MsG0780040157.01.T01:three_prime_UTR
CTGGTTACCGATGGCAACAT+TGG 0.489913 7:-73811164 MsG0780040157.01.T01:CDS
TGTTAGTTGTCTATTAGGCA+TGG 0.493299 7:-73810768 MsG0780040157.01.T01:three_prime_UTR
GATGGCCATGCTATGGGTGG+TGG 0.494326 7:-73811053 MsG0780040157.01.T01:CDS
GAAGTCCGTAAGCGTTGTTT+CGG 0.503695 7:-73812525 MsG0780040157.01.T01:CDS
CCTCTATAGCTATACAGAGT+TGG 0.504042 7:-73811433 MsG0780040157.01.T01:CDS
GTAACCAGTATAACAAGATC+TGG 0.510604 7:+73811179 None:intergenic
TAAAATGGATGGCCATGCTA+TGG 0.512856 7:-73811060 MsG0780040157.01.T01:CDS
TGATAATGATAGCCATAAGC+AGG 0.514260 7:-73811129 MsG0780040157.01.T01:CDS
TACCGATGGCAACATTGGCC+AGG 0.521808 7:-73811159 MsG0780040157.01.T01:CDS
CGACAAGATGAGCAAGGAAA+CGG 0.523192 7:-73812559 MsG0780040157.01.T01:CDS
CAAGGAAACGGTGATCGATA+AGG 0.526831 7:-73812547 MsG0780040157.01.T01:CDS
TAATAAGAGGATTCGATCGA+TGG 0.526977 7:+73812692 None:intergenic
ACGGATAGTAGGTCCGTTGT+TGG 0.528628 7:-73810306 MsG0780040157.01.T01:three_prime_UTR
CTCAGTCAGAGTCAAACAAA+TGG 0.530101 7:+73810633 None:intergenic
AGACCGATCCGACACCGGAT+AGG 0.540651 7:-73812742 None:intergenic
TGTTGCAGCAGCAAAGAGTC+CGG 0.542180 7:-73810849 MsG0780040157.01.T01:CDS
AACAAATGGTGCAACGATGG+AGG 0.544048 7:+73810647 None:intergenic
AACTTAGACCGATCCGACAC+CGG 0.546624 7:-73812747 None:intergenic
CACCAGTTCCTATGAATGAC+CGG 0.547874 7:+73810830 None:intergenic
CTGATTTGGGAACGAAAGAA+GGG 0.553462 7:+73812328 None:intergenic
TCTCGCCGAAACAACGCTTA+CGG 0.566509 7:+73812520 None:intergenic
CATGTCAAAATTGCTGTGAA+AGG 0.568758 7:-73811408 MsG0780040157.01.T01:CDS
TTTGCACAAAGTGTTGACAT+TGG 0.570113 7:-73811098 MsG0780040157.01.T01:CDS
TATCATTATCAAAACCATCC+TGG 0.572503 7:+73811141 None:intergenic
ACAAATGGTGCAACGATGGA+GGG 0.572588 7:+73810648 None:intergenic
CTTATAATTGTTGATGCGAG+TGG 0.573542 7:-73811354 MsG0780040157.01.T01:CDS
CGCATGTCTCGGGTGAACAT+GGG 0.573848 7:+73812465 None:intergenic
TAGGATGAGAGGTTGCTAGC+AGG 0.575905 7:+73810447 None:intergenic
GTAATTCTTACTAAAATGGA+TGG 0.579702 7:-73811071 MsG0780040157.01.T01:CDS
TCCTATAGATATAGGATGAG+AGG 0.581570 7:+73810436 None:intergenic
AAAACCCTAATCCGACGGAA+TGG 0.583898 7:-73812670 MsG0780040157.01.T01:five_prime_UTR
ACAGTTTGTCTGAAATGTGT+TGG 0.585711 7:-73810403 MsG0780040157.01.T01:three_prime_UTR
AGGAAGTGAAATCCCTGAGA+AGG 0.586219 7:+73810609 None:intergenic
ATAATTGTTGATGCGAGTGG+AGG 0.586828 7:-73811351 MsG0780040157.01.T01:CDS
TCAAACAAATGGTGCAACGA+TGG 0.591526 7:+73810644 None:intergenic
ATCCTGGCCAATGTTGCCAT+CGG 0.598390 7:+73811157 None:intergenic
GCACCAAATTTCAAAAGCAA+CGG 0.600294 7:-73811295 MsG0780040157.01.T01:intron
TAATTGTTGATGCGAGTGGA+GGG 0.603985 7:-73811350 MsG0780040157.01.T01:CDS
CTCTGACTGAGACCTTCTCA+GGG 0.609427 7:-73810621 MsG0780040157.01.T01:three_prime_UTR
GATGGCAACATTGGCCAGGA+TGG 0.609674 7:-73811155 MsG0780040157.01.T01:CDS
CTTGTTATACTGGTTACCGA+TGG 0.610312 7:-73811173 MsG0780040157.01.T01:CDS
TGATAGCCATAAGCAGGCCA+TGG 0.612027 7:-73811123 MsG0780040157.01.T01:CDS
AACAGATTACTCCATTCCGT+CGG 0.612317 7:+73812659 None:intergenic
AGCAAGCAAAATGGGTCTAG+CGG 0.613937 7:-73812610 MsG0780040157.01.T01:exon
GAGAACCACCACCCATAGCA+TGG 0.618730 7:+73811048 None:intergenic
GCCTAATAGACAACTAACAA+AGG 0.619483 7:+73810772 None:intergenic
AAAATGGATGGCCATGCTAT+GGG 0.624594 7:-73811059 MsG0780040157.01.T01:CDS
TTATTAAAACCCTAATCCGA+CGG 0.627030 7:-73812675 MsG0780040157.01.T01:five_prime_UTR
TGCTTTCGACAAGATGAGCA+AGG 0.633308 7:-73812565 MsG0780040157.01.T01:CDS
AACACTTTGTGCAAATGCCA+TGG 0.643369 7:+73811106 None:intergenic
TGGGTTCAATATAGGAACGA+AGG 0.650746 7:+73812484 None:intergenic
GAAAGGCGTGGAGAGATGCA+AGG 0.651781 7:-73811391 MsG0780040157.01.T01:CDS
CAAAATTGCTGTGAAAGGCG+TGG 0.655565 7:-73811403 MsG0780040157.01.T01:CDS
ATATCATATTTATCCAACAA+CGG 0.659134 7:+73810293 None:intergenic
GGAGGGAAATATGCAAGTGG+AGG 0.666347 7:+73810665 None:intergenic
ATGGATGGCCATGCTATGGG+TGG 0.678999 7:-73811056 MsG0780040157.01.T01:CDS
GATGGAGGGAAATATGCAAG+TGG 0.683679 7:+73810662 None:intergenic
TGTATAGCTATAGAGGAATG+CGG 0.694255 7:+73811440 None:intergenic
TAAATGGATAAAGATGAGCA+CGG 0.697225 7:-73810325 MsG0780040157.01.T01:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTCTGATATTAATAATTATA+AGG + Chr7:73812677-73812696 None:intergenic 10.0%
!! AAAATAACTTATATTCTGAT+AGG + Chr7:73811281-73811300 None:intergenic 15.0%
!! ATAAATTATAACATGAATGA+TGG + Chr7:73811114-73811133 None:intergenic 15.0%
!! TATGTAATGTTAATAAATTC+AGG - Chr7:73810846-73810865 MsG0780040157.01.T01:CDS 15.0%
!!! ATTTCTGTTTATATTTGTTT+TGG - Chr7:73812134-73812153 MsG0780040157.01.T01:CDS 15.0%
!!! TTTCTGTTTATATTTGTTTT+GGG - Chr7:73812135-73812154 MsG0780040157.01.T01:CDS 15.0%
!! AATGATGGTAAACAATATAA+AGG + Chr7:73811099-73811118 None:intergenic 20.0%
!! ATGATCATTCTTTATGTTTA+TGG - Chr7:73810805-73810824 MsG0780040157.01.T01:three_prime_UTR 20.0%
!! ATGATGGTAAACAATATAAA+GGG + Chr7:73811098-73811117 None:intergenic 20.0%
!! TTAAAAGTTCCTATAGATAT+AGG + Chr7:73812615-73812634 None:intergenic 20.0%
!! TTTATAACAAGAATGAAAAG+TGG + Chr7:73811455-73811474 None:intergenic 20.0%
!!! TATCTGTTTAGTCTTTTTAA+TGG - Chr7:73811379-73811398 MsG0780040157.01.T01:CDS 20.0%
!!! TCAACAATTGAATTTTTTAG+CGG + Chr7:73812069-73812088 None:intergenic 20.0%
! AGATATGAAGTTGCTAATAA+AGG + Chr7:73812454-73812473 None:intergenic 25.0%
! AGCTGTAATTCTTACTAAAA+TGG - Chr7:73811965-73811984 MsG0780040157.01.T01:intron 25.0%
! ATAACCAATATAACAAGATC+TGG + Chr7:73811804-73811823 None:intergenic 25.0%
! ATGATCATAACGTAATTAAC+AGG + Chr7:73810793-73810812 None:intergenic 25.0%
! ATTGATGAAGATGAAGATTA+GGG + Chr7:73810408-73810427 None:intergenic 25.0%
! GCAAATTATTATCAGAAGAA+AGG - Chr7:73810900-73810919 MsG0780040157.01.T01:intron 25.0%
! GTAATTCTTACTAAAATGGA+TGG - Chr7:73811969-73811988 MsG0780040157.01.T01:intron 25.0%
!!! ATGTTAATAAATTCAGGTTG+TGG - Chr7:73810852-73810871 MsG0780040157.01.T01:CDS 25.0%
!!! CTTTAAACTCTAAAGTGAAT+TGG + Chr7:73812033-73812052 None:intergenic 25.0%
AAAAATATTCTCTCGGAAGT+GGG - Chr7:73811157-73811176 MsG0780040157.01.T01:CDS 30.0%
AACAAGAATGAAAAGTGGTT+GGG + Chr7:73811450-73811469 None:intergenic 30.0%
AATTACCACTCTCTTAATAC+AGG + Chr7:73811251-73811270 None:intergenic 30.0%
AGTTTCCATAATCAAGCAAA+TGG + Chr7:73811515-73811534 None:intergenic 30.0%
ATAAAGGGTAGTAATGTTTC+AGG + Chr7:73811083-73811102 None:intergenic 30.0%
ATAACAAGATCTGGTATCAA+AGG + Chr7:73811795-73811814 None:intergenic 30.0%
ATCAATCAAGCAAGCAAAAT+GGG - Chr7:73810422-73810441 MsG0780040157.01.T01:three_prime_UTR 30.0%
ATTACCACTCTCTTAATACA+GGG + Chr7:73811250-73811269 None:intergenic 30.0%
ATTATCAAAACTCATCTGCA+AGG + Chr7:73811479-73811498 None:intergenic 30.0%
ATTCAACAAGTTGTGTTTGA+TGG - Chr7:73810665-73810684 MsG0780040157.01.T01:three_prime_UTR 30.0%
CATGTGCAAAAATATTCTCT+CGG - Chr7:73811150-73811169 MsG0780040157.01.T01:CDS 30.0%
GAATGTAACTTTCCTTTGTT+TGG - Chr7:73811185-73811204 MsG0780040157.01.T01:CDS 30.0%
GATTGATGAAGATGAAGATT+AGG + Chr7:73810409-73810428 None:intergenic 30.0%
TAAAGGGTAGTAATGTTTCA+GGG + Chr7:73811082-73811101 None:intergenic 30.0%
TAAATGGATAAAGATGAGCA+CGG - Chr7:73812715-73812734 MsG0780040157.01.T01:five_prime_UTR 30.0%
TAACAAGAATGAAAAGTGGT+TGG + Chr7:73811451-73811470 None:intergenic 30.0%
TACTACCATTTGCTTGATTA+TGG - Chr7:73811507-73811526 MsG0780040157.01.T01:intron 30.0%
TATATACCAGAACCAAACAA+AGG + Chr7:73811200-73811219 None:intergenic 30.0%
TATCATTATCAAAACCATCC+TGG + Chr7:73811902-73811921 None:intergenic 30.0%
TGTGCAGATACATTTAGAAA+TGG - Chr7:73810942-73810961 MsG0780040157.01.T01:intron 30.0%
TTATTAAAACCCTAATCCGA+CGG - Chr7:73810365-73810384 MsG0780040157.01.T01:three_prime_UTR 30.0%
! CTTGTTATATTGGTTATCGA+TGG - Chr7:73811807-73811826 MsG0780040157.01.T01:intron 30.0%
! CTTTTCTTTTCTTTCCTATC+CGG + Chr7:73810315-73810334 None:intergenic 30.0%
! GATACCAGATCTTGTTATAT+TGG - Chr7:73811797-73811816 MsG0780040157.01.T01:intron 30.0%
!!! AACTTTGCCTTATTTTCTCA+GGG - Chr7:73811354-73811373 MsG0780040157.01.T01:CDS 30.0%
!!! ACTTTTCGTTGATTTCTAGT+TGG - Chr7:73811406-73811425 MsG0780040157.01.T01:CDS 30.0%
!!! ATACCGTTGCTTTTGAAATT+TGG + Chr7:73811751-73811770 None:intergenic 30.0%
!!! CTTTTCGTTGATTTCTAGTT+GGG - Chr7:73811407-73811426 MsG0780040157.01.T01:CDS 30.0%
!!! TATTTGTTTTGGGTACATGT+TGG - Chr7:73812145-73812164 MsG0780040157.01.T01:CDS 30.0%
!!! TTTTCGTTGATTTCTAGTTG+GGG - Chr7:73811408-73811427 MsG0780040157.01.T01:CDS 30.0%
AAAGCTAACATCCCTTGTTA+TGG - Chr7:73810995-73811014 MsG0780040157.01.T01:intron 35.0%
ACCTCTCATCCTATATCTAT+AGG - Chr7:73812603-73812622 MsG0780040157.01.T01:CDS 35.0%
AGATAAGCCCTGAGAAAATA+AGG + Chr7:73811364-73811383 None:intergenic 35.0%
CAAAAATATTCTCTCGGAAG+TGG - Chr7:73811156-73811175 MsG0780040157.01.T01:CDS 35.0%
CAGAAGAAAGGATTAAAGAC+AGG - Chr7:73810912-73810931 MsG0780040157.01.T01:intron 35.0%
CATAACGTAATTAACAGGTC+AGG + Chr7:73810788-73810807 None:intergenic 35.0%
CATCAATCAAGCAAGCAAAA+TGG - Chr7:73810421-73810440 MsG0780040157.01.T01:three_prime_UTR 35.0%
CATGTCAAAATTGCTGTGAA+AGG - Chr7:73811632-73811651 MsG0780040157.01.T01:intron 35.0%
CGTTTGCTTCTTCTGAATAA+TGG - Chr7:73812556-73812575 MsG0780040157.01.T01:CDS 35.0%
CTTATAATTGTTGATGCGAG+TGG - Chr7:73811686-73811705 MsG0780040157.01.T01:intron 35.0%
GAAAGGATTAAAGACAGGTT+TGG - Chr7:73810917-73810936 MsG0780040157.01.T01:intron 35.0%
GCACCAAATTTCAAAAGCAA+CGG - Chr7:73811745-73811764 MsG0780040157.01.T01:intron 35.0%
GCCTAATAGACAACTAACAA+AGG + Chr7:73812271-73812290 None:intergenic 35.0%
GCCTTTGTTAGTTGTCTATT+AGG - Chr7:73812267-73812286 MsG0780040157.01.T01:intron 35.0%
GTAACCAGTATAACAAGATC+TGG + Chr7:73811864-73811883 None:intergenic 35.0%
GTATGTACCTTCCATAACAA+GGG + Chr7:73811009-73811028 None:intergenic 35.0%
TAATAAGAGGATTCGATCGA+TGG + Chr7:73810351-73810370 None:intergenic 35.0%
TCCTATAGATATAGGATGAG+AGG + Chr7:73812607-73812626 None:intergenic 35.0%
TGTATAGCTATAGAGGAATG+CGG + Chr7:73811603-73811622 None:intergenic 35.0%
TGTTAGTTGTCTATTAGGCA+TGG - Chr7:73812272-73812291 MsG0780040157.01.T01:intron 35.0%
TTACCACTCTCTTAATACAG+GGG + Chr7:73811249-73811268 None:intergenic 35.0%
TTCTCTTTATAGTTGCGATG+AGG + Chr7:73811336-73811355 None:intergenic 35.0%
! AACTTTCCTTTGTTTGGTTC+TGG - Chr7:73811191-73811210 MsG0780040157.01.T01:intron 35.0%
! ACAAGTGTTGTCATGTTTGT+TGG - Chr7:73810740-73810759 MsG0780040157.01.T01:three_prime_UTR 35.0%
! ACAGTTTGTCTGAAATGTGT+TGG - Chr7:73812637-73812656 MsG0780040157.01.T01:five_prime_UTR 35.0%
! ATATCAGAAGTGTGTGAGTT+AGG - Chr7:73812688-73812707 MsG0780040157.01.T01:five_prime_UTR 35.0%
! GAAACCAGATCTTGTTATAC+TGG - Chr7:73811857-73811876 MsG0780040157.01.T01:intron 35.0%
! TATCAGAAGTGTGTGAGTTA+GGG - Chr7:73812689-73812708 MsG0780040157.01.T01:five_prime_UTR 35.0%
! TGATAATGATAGCCATAAGC+AGG - Chr7:73811911-73811930 MsG0780040157.01.T01:intron 35.0%
!! AACACTTGTTTTCCCTGATT+TGG + Chr7:73810729-73810748 None:intergenic 35.0%
!! ACACTTGTTTTCCCTGATTT+GGG + Chr7:73810728-73810747 None:intergenic 35.0%
!! CGAGAGAATATTTTTGCACA+TGG + Chr7:73811152-73811171 None:intergenic 35.0%
!! CGGATTAGGGTTTTAATAAG+AGG + Chr7:73810364-73810383 None:intergenic 35.0%
!! TTTGCACAAAGTGTTGACAT+TGG - Chr7:73811942-73811961 MsG0780040157.01.T01:intron 35.0%
!!! GAACTTTGCCTTATTTTCTC+AGG - Chr7:73811353-73811372 MsG0780040157.01.T01:CDS 35.0%
AAAATGGATGGCCATGCTAT+GGG - Chr7:73811981-73812000 MsG0780040157.01.T01:intron 40.0%
AACACTTTGTGCAAATGCCA+TGG + Chr7:73811937-73811956 None:intergenic 40.0%
AACAGATTACTCCATTCCGT+CGG + Chr7:73810384-73810403 None:intergenic 40.0%
AATGAAAAGTGGTTGGGCAA+TGG + Chr7:73811444-73811463 None:intergenic 40.0%
ATAATTGTTGATGCGAGTGG+AGG - Chr7:73811689-73811708 MsG0780040157.01.T01:intron 40.0%
ATGAAAAGTGGTTGGGCAAT+GGG + Chr7:73811443-73811462 None:intergenic 40.0%
CCAACTCTGTATAGCTATAG+AGG + Chr7:73811610-73811629 None:intergenic 40.0%
CCTCTATAGCTATACAGAGT+TGG - Chr7:73811607-73811626 MsG0780040157.01.T01:intron 40.0%
CGTATGTACCTTCCATAACA+AGG + Chr7:73811010-73811029 None:intergenic 40.0%
CTCAGTCAGAGTCAAACAAA+TGG + Chr7:73812410-73812429 None:intergenic 40.0%
CTTCTTTCGTTCCCAAATCA+GGG - Chr7:73810714-73810733 MsG0780040157.01.T01:three_prime_UTR 40.0%
GGTGAACATGGGTTCAATAT+AGG + Chr7:73810567-73810586 None:intergenic 40.0%
GTGTGAGTTAGGGATGTAAA+TGG - Chr7:73812699-73812718 MsG0780040157.01.T01:five_prime_UTR 40.0%
TAAAATGGATGGCCATGCTA+TGG - Chr7:73811980-73811999 MsG0780040157.01.T01:intron 40.0%
TAAAGATGAGCACGGATAGT+AGG - Chr7:73812723-73812742 MsG0780040157.01.T01:five_prime_UTR 40.0%
TAATTGTTGATGCGAGTGGA+GGG - Chr7:73811690-73811709 MsG0780040157.01.T01:intron 40.0%
TCAAACAAATGGTGCAACGA+TGG + Chr7:73812399-73812418 None:intergenic 40.0%
TCATAGGAACTGGTGACAAA+TGG - Chr7:73812218-73812237 MsG0780040157.01.T01:intron 40.0%
TGAGAGTGGGAAGTTAAAGT+TGG + Chr7:73811313-73811332 None:intergenic 40.0%
TGGGTTCAATATAGGAACGA+AGG + Chr7:73810559-73810578 None:intergenic 40.0%
! CTAACATCCCTTGTTATGGA+AGG - Chr7:73810999-73811018 MsG0780040157.01.T01:intron 40.0%
! CTTGTTATACTGGTTACCGA+TGG - Chr7:73811867-73811886 MsG0780040157.01.T01:intron 40.0%
! GGTATCCAGACTTTTCTTTC+TGG + Chr7:73812357-73812376 None:intergenic 40.0%
! TTTTCTTTCCTATCCGGTGT+CGG + Chr7:73810309-73810328 None:intergenic 40.0%
!! ACAAGGGATGTTAGCTTTAG+TGG + Chr7:73810993-73811012 None:intergenic 40.0%
!! CTGATTTGGGAACGAAAGAA+GGG + Chr7:73810715-73810734 None:intergenic 40.0%
!! TGATGGACTCTGCAAATTGT+TGG - Chr7:73810682-73810701 MsG0780040157.01.T01:three_prime_UTR 40.0%
!! TGATTTTAGCACGCATGTCT+CGG + Chr7:73810589-73810608 None:intergenic 40.0%
!! TTTCTTTCTGGCAGGTTTTG+AGG + Chr7:73812345-73812364 None:intergenic 40.0%
!!! AAAATGGGTCTAGCGGATTT+TGG - Chr7:73810437-73810456 MsG0780040157.01.T01:three_prime_UTR 40.0%
AAAACCCTAATCCGACGGAA+TGG - Chr7:73810370-73810389 MsG0780040157.01.T01:three_prime_UTR 45.0%
AACAAATGGTGCAACGATGG+AGG + Chr7:73812396-73812415 None:intergenic 45.0%
ACAAATGGTGCAACGATGGA+GGG + Chr7:73812395-73812414 None:intergenic 45.0%
ACCTGCCAGAAAGAAAAGTC+TGG - Chr7:73812349-73812368 MsG0780040157.01.T01:CDS 45.0%
AGCAAGCAAAATGGGTCTAG+CGG - Chr7:73810430-73810449 MsG0780040157.01.T01:three_prime_UTR 45.0%
AGGAAGTGAAATCCCTGAGA+AGG + Chr7:73812434-73812453 None:intergenic 45.0%
CAAAATTGCTGTGAAAGGCG+TGG - Chr7:73811637-73811656 MsG0780040157.01.T01:intron 45.0%
CAAAGAGTCCGGTCATTCAT+AGG - Chr7:73812202-73812221 MsG0780040157.01.T01:intron 45.0%
CAAGGAAACGGTGATCGATA+AGG - Chr7:73810493-73810512 MsG0780040157.01.T01:three_prime_UTR 45.0%
CACCAGTTCCTATGAATGAC+CGG + Chr7:73812213-73812232 None:intergenic 45.0%
CATCCCCTGTATTAAGAGAG+TGG - Chr7:73811243-73811262 MsG0780040157.01.T01:intron 45.0%
CCTTCTTTCGTTCCCAAATC+AGG - Chr7:73810713-73810732 MsG0780040157.01.T01:three_prime_UTR 45.0%
CGACAAGATGAGCAAGGAAA+CGG - Chr7:73810481-73810500 MsG0780040157.01.T01:three_prime_UTR 45.0%
GATGGAGGGAAATATGCAAG+TGG + Chr7:73812381-73812400 None:intergenic 45.0%
GTCTATTAGGCATGGATGAC+TGG - Chr7:73812280-73812299 MsG0780040157.01.T01:intron 45.0%
TACTCCATTCCGTCGGATTA+GGG + Chr7:73810377-73810396 None:intergenic 45.0%
TGCTTTCGACAAGATGAGCA+AGG - Chr7:73810475-73810494 MsG0780040157.01.T01:three_prime_UTR 45.0%
TTACTCCATTCCGTCGGATT+AGG + Chr7:73810378-73810397 None:intergenic 45.0%
TTGGGAACGAAAGAAGGGTT+AGG + Chr7:73810710-73810729 None:intergenic 45.0%
! GAAGTCCGTAAGCGTTGTTT+CGG - Chr7:73810515-73810534 MsG0780040157.01.T01:three_prime_UTR 45.0%
! GTCATGTTTGTTGGTGCTCA+AGG - Chr7:73810749-73810768 MsG0780040157.01.T01:three_prime_UTR 45.0%
!! ATTCTCTCGGAAGTGGGTTT+TGG - Chr7:73811163-73811182 MsG0780040157.01.T01:CDS 45.0%
!! CCTGATTTGGGAACGAAAGA+AGG + Chr7:73810716-73810735 None:intergenic 45.0%
!! GATTTTAGCACGCATGTCTC+GGG + Chr7:73810588-73810607 None:intergenic 45.0%
!! TCCAGACTTTTCTTTCTGGC+AGG + Chr7:73812353-73812372 None:intergenic 45.0%
ACGGATAGTAGGTCCGTTGT+TGG - Chr7:73812734-73812753 MsG0780040157.01.T01:five_prime_UTR 50.0%
ACTCTGACTGAGACCTTCTC+AGG - Chr7:73812418-73812437 MsG0780040157.01.T01:CDS 50.0%
AGTTGCGATGAGGTGAGAGT+GGG + Chr7:73811326-73811345 None:intergenic 50.0%
ATCCTGGCCAATGTTGCCAT+CGG + Chr7:73811886-73811905 None:intergenic 50.0%
CAAATGCCATGGCCTGCTTA+TGG + Chr7:73811926-73811945 None:intergenic 50.0%
CTCTGACTGAGACCTTCTCA+GGG - Chr7:73812419-73812438 MsG0780040157.01.T01:CDS 50.0%
CTGGTTACCGATGGCAACAT+TGG - Chr7:73811876-73811895 MsG0780040157.01.T01:intron 50.0%
GGAGGGAAATATGCAAGTGG+AGG + Chr7:73812378-73812397 None:intergenic 50.0%
GTCCGGTCATTCATAGGAAC+TGG - Chr7:73812208-73812227 MsG0780040157.01.T01:intron 50.0%
TAGGATGAGAGGTTGCTAGC+AGG + Chr7:73812596-73812615 None:intergenic 50.0%
TAGTTGCGATGAGGTGAGAG+TGG + Chr7:73811327-73811346 None:intergenic 50.0%
TCTCGCCGAAACAACGCTTA+CGG + Chr7:73810523-73810542 None:intergenic 50.0%
TGATAGCCATAAGCAGGCCA+TGG - Chr7:73811917-73811936 MsG0780040157.01.T01:intron 50.0%
TGTTGCAGCAGCAAAGAGTC+CGG - Chr7:73812191-73812210 MsG0780040157.01.T01:intron 50.0%
! AGCGTTGTTTCGGCGAGATT+AGG - Chr7:73810525-73810544 MsG0780040157.01.T01:three_prime_UTR 50.0%
!!! GGCAGGTTTTGAGGCAATTC+AGG + Chr7:73812336-73812355 None:intergenic 50.0%
AAGTGGTTGGGCAATGGGCA+TGG + Chr7:73811438-73811457 None:intergenic 55.0%
ACGCATGTCTCGGGTGAACA+TGG + Chr7:73810579-73810598 None:intergenic 55.0%
ATGGATGGCCATGCTATGGG+TGG - Chr7:73811984-73812003 MsG0780040157.01.T01:intron 55.0%
CGCATGTCTCGGGTGAACAT+GGG + Chr7:73810578-73810597 None:intergenic 55.0%
GAGAACCACCACCCATAGCA+TGG + Chr7:73811995-73812014 None:intergenic 55.0%
GATGGCAACATTGGCCAGGA+TGG - Chr7:73811885-73811904 MsG0780040157.01.T01:intron 55.0%
TACCGATGGCAACATTGGCC+AGG - Chr7:73811881-73811900 MsG0780040157.01.T01:intron 55.0%
! GAAAGGCGTGGAGAGATGCA+AGG - Chr7:73811649-73811668 MsG0780040157.01.T01:intron 55.0%
GATGGCCATGCTATGGGTGG+TGG - Chr7:73811987-73812006 MsG0780040157.01.T01:intron 60.0%
Chromosome Type Strat End Strand Name
Chr7 gene 73810304 73812758 73810304 ID=MsG0780040157.01;Name=MsG0780040157.01
Chr7 mRNA 73810304 73812758 73810304 ID=MsG0780040157.01.T01;Parent=MsG0780040157.01;Name=MsG0780040157.01.T01;_AED=0.49;_eAED=0.49;_QI=136|0.75|0.6|1|0.75|0.8|5|525|256
Chr7 exon 73810304 73810871 73810304 ID=MsG0780040157.01.T01:exon:13309;Parent=MsG0780040157.01.T01
Chr7 exon 73811041 73811204 73811041 ID=MsG0780040157.01.T01:exon:13308;Parent=MsG0780040157.01.T01
Chr7 exon 73811296 73811462 73811296 ID=MsG0780040157.01.T01:exon:13307;Parent=MsG0780040157.01.T01
Chr7 exon 73812129 73812194 73812129 ID=MsG0780040157.01.T01:exon:13306;Parent=MsG0780040157.01.T01
Chr7 exon 73812292 73812758 73812292 ID=MsG0780040157.01.T01:exon:13305;Parent=MsG0780040157.01.T01
Chr7 five_prime_UTR 73812623 73812758 73812623 ID=MsG0780040157.01.T01:five_prime_utr;Parent=MsG0780040157.01.T01
Chr7 CDS 73812292 73812622 73812292 ID=MsG0780040157.01.T01:cds;Parent=MsG0780040157.01.T01
Chr7 CDS 73812129 73812194 73812129 ID=MsG0780040157.01.T01:cds;Parent=MsG0780040157.01.T01
Chr7 CDS 73811296 73811462 73811296 ID=MsG0780040157.01.T01:cds;Parent=MsG0780040157.01.T01
Chr7 CDS 73811041 73811204 73811041 ID=MsG0780040157.01.T01:cds;Parent=MsG0780040157.01.T01
Chr7 CDS 73810829 73810871 73810829 ID=MsG0780040157.01.T01:cds;Parent=MsG0780040157.01.T01
Chr7 three_prime_UTR 73810304 73810828 73810304 ID=MsG0780040157.01.T01:three_prime_utr;Parent=MsG0780040157.01.T01
Gene Sequence

>MsG0780040157.01.T01

ATGGGTCTAGCGGATTTTGGAGAGAGTATTTTGCGTGCTTTCGACAAGATGAGCAAGGAAACGGTGATCGATAAGGAAGTCCGTAAGCGTTGTTTCGGCGAGATTAGGCTAGCTTTCCTTCGTTCCTATATTGAACCCATGTTCACCCGAGACATGCGTGCTAAAATCAAGAACATTCTCATCAATCATCTTAATCTTGATGATCTCGACGCTGCTCGTAAGATGATTCAACAAGTTGTGTTTGATGGACTCTGCAAATTGTTGGAACCTAACCCTTCTTTCGTTCCCAAATCAGGGAAAACAAGTGTTGTCATGTTTGTTGGTGCTCAAGGTTGTGGAAAAACAACAACTTGTATACAATATGCAAATTATTATCAGAAGAAAGGATTAAAGACAGCCGCATTCCTCTATAGCTATACAGAGTTGGATCATGTCAAAATTGCTGTGAAAGGCGTGGAGAGATGCAAGGAAGAAAACTGTGATCTTATAATTGTTGATGCGAGTGGAGGGCACAAACAAGAAGATGCTCTTTTTGAAGAAATGCACCAAATTTCAAAAGCAACGAAACCAGATCTTGTTATACTGGTTACCGATGGCAACATTGGCCAGGATGGTTTTGATAATGATAGCCATAAGCAGGCCATGGCATTTGCACAAAGTGTTGACATTGGAGCTGTAATTCTTACTAAAATGGATGGCCATGCTATGGGTGGTGGTTCTCTTAGCGCTGTTGCAGCAGCAAAGAGTCCGGTCATTCATAGGAACTGGTGA
Protein sequence

>MsG0780040157.01.T01

MGLADFGESILRAFDKMSKETVIDKEVRKRCFGEIRLAFLRSYIEPMFTRDMRAKIKNILINHLNLDDLDAARKMIQQVVFDGLCKLLEPNPSFVPKSGKTSVVMFVGAQGCGKTTTCIQYANYYQKKGLKTAAFLYSYTELDHVKIAVKGVERCKEENCDLIIVDASGGHKQEDALFEEMHQISKATKPDLVILVTDGNIGQDGFDNDSHKQAMAFAQSVDIGAVILTKMDGHAMGGGSLSAVAAAKSPVIHRNW*