Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780040231.01.T01 | AAD56017.1 | 100 | 136 | 0 | 0 | 1 | 136 | 1 | 136 | 6.47E-80 | 241 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780040231.01.T01 | Q9SPB5 | 100.000 | 136 | 0 | 0 | 1 | 136 | 1 | 136 | 3.09e-80 | 241 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180003379.01 | MsG0780040231.01 | 0.813157 | 2.950337e-51 | 1.911857e-48 |
| MsG0780040208.01 | MsG0780040231.01 | 0.901877 | 1.717125e-78 | 2.367799e-74 |
| MsG0780040231.01 | MsG0780041454.01 | 0.827253 | 1.732720e-54 | 1.663862e-51 |
| MsG0780040231.01 | MsG0880046068.01 | 0.824749 | 6.818610e-54 | 6.094671e-51 |
| MsG0480022248.01 | MsG0780040231.01 | 0.809613 | 1.735383e-50 | 1.023728e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780040231.01.T01 | MTR_7g086340 | 81.452 | 124 | 8 | 1 | 1 | 124 | 1 | 109 | 3.20e-27 | 98.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 44 sgRNAs with CRISPR-Local
Find 51 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCTCCTTGTGCTCTTCCCTT+TGG | 0.329912 | 7:+74674980 | None:intergenic |
| AACCATGTTGTTCTCCTTTG+TGG | 0.347404 | 7:+74675076 | None:intergenic |
| AAAGGAGAGCAACATGGATT+TGG | 0.348363 | 7:-74675027 | MsG0780040231.01.T01:CDS |
| GGAGAACAACATGGATTTGT+TGG | 0.387551 | 7:-74675114 | MsG0780040231.01.T01:CDS |
| TGTCACTGCTGCTGCTATCA+TGG | 0.437381 | 7:+74674854 | None:intergenic |
| AAGATCAAGGACAAAATTCA+TGG | 0.463252 | 7:-74674946 | MsG0780040231.01.T01:CDS |
| CTTGTCTCCTCCTACGTGAA+GGG | 0.488043 | 7:+74675203 | None:intergenic |
| TCTTGTCTCCTCCTACGTGA+AGG | 0.500204 | 7:+74675202 | None:intergenic |
| GCAGGAATCATGAACAAGAT+TGG | 0.500782 | 7:-74675231 | MsG0780040231.01.T01:CDS |
| CATGATGTTCTCCTCCTACA+TGG | 0.514204 | 7:+74675148 | None:intergenic |
| AAGAAAGAGAAGAAGAAACA+TGG | 0.530177 | 7:-74674895 | MsG0780040231.01.T01:CDS |
| GGATTTGTTGGAGGACATGC+TGG | 0.539276 | 7:-74675102 | MsG0780040231.01.T01:CDS |
| GGGATTTGCCGACAAGATCA+AGG | 0.551089 | 7:-74674959 | MsG0780040231.01.T01:CDS |
| ATACCAAAGGGAAGAGCACA+AGG | 0.553678 | 7:-74674983 | MsG0780040231.01.T01:CDS |
| CCCTTTGGTATCCCTCCTTG+TGG | 0.553926 | 7:+74674995 | None:intergenic |
| CGTAGGAGGAGACAAGAAAG+AGG | 0.554208 | 7:-74675196 | MsG0780040231.01.T01:CDS |
| AAATAGAAATACAATATGGC+AGG | 0.556347 | 7:-74675249 | None:intergenic |
| GAGAAGAAGAAACATGGAGA+GGG | 0.558354 | 7:-74674889 | MsG0780040231.01.T01:CDS |
| GGAGAACAACATGGCCATGT+AGG | 0.558430 | 7:-74675162 | MsG0780040231.01.T01:CDS |
| CATGGAGAGGGTCATGAACA+TGG | 0.563434 | 7:-74674877 | MsG0780040231.01.T01:CDS |
| GAACAACATGGATTTGTTGG+AGG | 0.585288 | 7:-74675111 | MsG0780040231.01.T01:CDS |
| CCACAAGGAGGGATACCAAA+GGG | 0.586545 | 7:-74674995 | MsG0780040231.01.T01:CDS |
| AAGGACAAAATTCATGGTGA+AGG | 0.593365 | 7:-74674940 | MsG0780040231.01.T01:CDS |
| GAGCAACATGGATTTGGTCA+TGG | 0.593720 | 7:-74675021 | MsG0780040231.01.T01:CDS |
| GAACAACATGGCCATGTAGG+AGG | 0.600098 | 7:-74675159 | MsG0780040231.01.T01:CDS |
| AAGAAAGAGGGAGAACACAA+AGG | 0.609599 | 7:-74675183 | MsG0780040231.01.T01:CDS |
| ATTCATGGTGAAGGTGCAGA+TGG | 0.614174 | 7:-74674931 | MsG0780040231.01.T01:CDS |
| CATGTAGGAGGAGAACATCA+TGG | 0.615237 | 7:-74675147 | MsG0780040231.01.T01:CDS |
| GGTGACGCCCTTCACGTAGG+AGG | 0.615290 | 7:-74675210 | MsG0780040231.01.T01:CDS |
| CAAAGGGAAGAGCACAAGGA+GGG | 0.635121 | 7:-74674979 | MsG0780040231.01.T01:CDS |
| GTAGGAGGAGACAAGAAAGA+GGG | 0.644708 | 7:-74675195 | MsG0780040231.01.T01:CDS |
| ATTTGGTCATGGAGACCACA+AGG | 0.646260 | 7:-74675010 | MsG0780040231.01.T01:CDS |
| GAACACAAAGGAGAACAACA+TGG | 0.647088 | 7:-74675171 | MsG0780040231.01.T01:CDS |
| GATTACAAAGGAGAGCAACA+TGG | 0.647436 | 7:-74675033 | MsG0780040231.01.T01:CDS |
| GGTCATGGAGACCACAAGGA+GGG | 0.647519 | 7:-74675006 | MsG0780040231.01.T01:CDS |
| ACCACAAGGAGGGATACCAA+AGG | 0.651748 | 7:-74674996 | MsG0780040231.01.T01:CDS |
| AGAGAAGAAGAAACATGGAG+AGG | 0.656957 | 7:-74674890 | MsG0780040231.01.T01:CDS |
| GAGTACAAAGGAGAACAACA+TGG | 0.667533 | 7:-74675123 | MsG0780040231.01.T01:CDS |
| GAACATCATGGTGAGTACAA+AGG | 0.675462 | 7:-74675135 | MsG0780040231.01.T01:CDS |
| GGACATGCTGGTGACCACAA+AGG | 0.680130 | 7:-74675090 | MsG0780040231.01.T01:CDS |
| GACCACAAAGGAGAACAACA+TGG | 0.705961 | 7:-74675078 | MsG0780040231.01.T01:CDS |
| TGGTCATGGAGACCACAAGG+AGG | 0.712485 | 7:-74675007 | MsG0780040231.01.T01:CDS |
| CCAAAGGGAAGAGCACAAGG+AGG | 0.721733 | 7:-74674980 | MsG0780040231.01.T01:CDS |
| ATTGGTGACGCCCTTCACGT+AGG | 0.721789 | 7:-74675213 | MsG0780040231.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAGAAAGAGAAGAAGAAACA+TGG | - | Chr7:74675185-74675204 | MsG0780040231.01.T01:CDS | 30.0% | |
| AAGATCAAGGACAAAATTCA+TGG | - | Chr7:74675134-74675153 | MsG0780040231.01.T01:CDS | 30.0% | |
| ! | AATTTTGTCCTTGATCTTGT+CGG | + | Chr7:74675132-74675151 | None:intergenic | 30.0% |
| AAGGACAAAATTCATGGTGA+AGG | - | Chr7:74675140-74675159 | MsG0780040231.01.T01:CDS | 35.0% | |
| AAAGGAGAGCAACATGGATT+TGG | - | Chr7:74675053-74675072 | MsG0780040231.01.T01:CDS | 40.0% | |
| AACCATGTTGTTCTCCTTTG+TGG | + | Chr7:74675007-74675026 | None:intergenic | 40.0% | |
| AAGAAAGAGGGAGAACACAA+AGG | - | Chr7:74674897-74674916 | MsG0780040231.01.T01:CDS | 40.0% | |
| AGAGAAGAAGAAACATGGAG+AGG | - | Chr7:74675190-74675209 | MsG0780040231.01.T01:CDS | 40.0% | |
| GAACACAAAGGAGAACAACA+TGG | - | Chr7:74674909-74674928 | MsG0780040231.01.T01:CDS | 40.0% | |
| GAACATCATGGTGAGTACAA+AGG | - | Chr7:74674945-74674964 | MsG0780040231.01.T01:CDS | 40.0% | |
| GAGAAGAAGAAACATGGAGA+GGG | - | Chr7:74675191-74675210 | MsG0780040231.01.T01:CDS | 40.0% | |
| GAGTACAAAGGAGAACAACA+TGG | - | Chr7:74674957-74674976 | MsG0780040231.01.T01:CDS | 40.0% | |
| GATTACAAAGGAGAGCAACA+TGG | - | Chr7:74675047-74675066 | MsG0780040231.01.T01:CDS | 40.0% | |
| GCAGGAATCATGAACAAGAT+TGG | - | Chr7:74674849-74674868 | MsG0780040231.01.T01:CDS | 40.0% | |
| GGAGAACAACATGGATTTGT+TGG | - | Chr7:74674966-74674985 | MsG0780040231.01.T01:CDS | 40.0% | |
| !! | GAACAACATGGATTTGTTGG+AGG | - | Chr7:74674969-74674988 | MsG0780040231.01.T01:CDS | 40.0% |
| !!! | ATGGTTTTGTAGGAGGACAT+GGG | - | Chr7:74675021-74675040 | MsG0780040231.01.T01:CDS | 40.0% |
| !!! | GAACAACATGGTTTTGTAGG+AGG | - | Chr7:74675014-74675033 | MsG0780040231.01.T01:CDS | 40.0% |
| !!! | GGAGAACAACATGGTTTTGT+AGG | - | Chr7:74675011-74675030 | MsG0780040231.01.T01:CDS | 40.0% |
| ATTTGGTCATGGAGACCACA+AGG | - | Chr7:74675070-74675089 | MsG0780040231.01.T01:CDS | 45.0% | |
| CATGATGTTCTCCTCCTACA+TGG | + | Chr7:74674935-74674954 | None:intergenic | 45.0% | |
| CATGTAGGAGGAGAACATCA+TGG | - | Chr7:74674933-74674952 | MsG0780040231.01.T01:CDS | 45.0% | |
| GACCACAAAGGAGAACAACA+TGG | - | Chr7:74675002-74675021 | MsG0780040231.01.T01:CDS | 45.0% | |
| GTAGGAGGAGACAAGAAAGA+GGG | - | Chr7:74674885-74674904 | MsG0780040231.01.T01:CDS | 45.0% | |
| ! | ATACCAAAGGGAAGAGCACA+AGG | - | Chr7:74675097-74675116 | MsG0780040231.01.T01:CDS | 45.0% |
| ! | ATTCATGGTGAAGGTGCAGA+TGG | - | Chr7:74675149-74675168 | MsG0780040231.01.T01:CDS | 45.0% |
| ! | GAGCAACATGGATTTGGTCA+TGG | - | Chr7:74675059-74675078 | MsG0780040231.01.T01:CDS | 45.0% |
| !!! | CATGGTTTTGTAGGAGGACA+TGG | - | Chr7:74675020-74675039 | MsG0780040231.01.T01:CDS | 45.0% |
| !!! | TGGTTTTGTAGGAGGACATG+GGG | - | Chr7:74675022-74675041 | MsG0780040231.01.T01:CDS | 45.0% |
| ACCACAAGGAGGGATACCAA+AGG | - | Chr7:74675084-74675103 | MsG0780040231.01.T01:CDS | 50.0% | |
| CATGGAGAGGGTCATGAACA+TGG | - | Chr7:74675203-74675222 | MsG0780040231.01.T01:CDS | 50.0% | |
| CCACAAGGAGGGATACCAAA+GGG | - | Chr7:74675085-74675104 | MsG0780040231.01.T01:CDS | 50.0% | |
| CGTAGGAGGAGACAAGAAAG+AGG | - | Chr7:74674884-74674903 | MsG0780040231.01.T01:CDS | 50.0% | |
| CTTGTCTCCTCCTACGTGAA+GGG | + | Chr7:74674880-74674899 | None:intergenic | 50.0% | |
| GAACAACATGGCCATGTAGG+AGG | - | Chr7:74674921-74674940 | MsG0780040231.01.T01:CDS | 50.0% | |
| GGACATGGGGGTGATTACAA+AGG | - | Chr7:74675035-74675054 | MsG0780040231.01.T01:CDS | 50.0% | |
| GGAGAACAACATGGCCATGT+AGG | - | Chr7:74674918-74674937 | MsG0780040231.01.T01:CDS | 50.0% | |
| GGGATTTGCCGACAAGATCA+AGG | - | Chr7:74675121-74675140 | MsG0780040231.01.T01:CDS | 50.0% | |
| TCTTGTCTCCTCCTACGTGA+AGG | + | Chr7:74674881-74674900 | None:intergenic | 50.0% | |
| TGTCACTGCTGCTGCTATCA+TGG | + | Chr7:74675229-74675248 | None:intergenic | 50.0% | |
| ! | CAAAGGGAAGAGCACAAGGA+GGG | - | Chr7:74675101-74675120 | MsG0780040231.01.T01:CDS | 50.0% |
| !! | GGATTTGTTGGAGGACATGC+TGG | - | Chr7:74674978-74674997 | MsG0780040231.01.T01:CDS | 50.0% |
| !! | GGTTTTGTAGGAGGACATGG+GGG | - | Chr7:74675023-74675042 | MsG0780040231.01.T01:CDS | 50.0% |
| CCCTTTGGTATCCCTCCTTG+TGG | + | Chr7:74675088-74675107 | None:intergenic | 55.0% | |
| CCTCCTTGTGCTCTTCCCTT+TGG | + | Chr7:74675103-74675122 | None:intergenic | 55.0% | |
| GGACATGCTGGTGACCACAA+AGG | - | Chr7:74674990-74675009 | MsG0780040231.01.T01:CDS | 55.0% | |
| GGTCATGGAGACCACAAGGA+GGG | - | Chr7:74675074-74675093 | MsG0780040231.01.T01:CDS | 55.0% | |
| TGGTCATGGAGACCACAAGG+AGG | - | Chr7:74675073-74675092 | MsG0780040231.01.T01:CDS | 55.0% | |
| ! | CCAAAGGGAAGAGCACAAGG+AGG | - | Chr7:74675100-74675119 | MsG0780040231.01.T01:CDS | 55.0% |
| !! | ATTGGTGACGCCCTTCACGT+AGG | - | Chr7:74674867-74674886 | MsG0780040231.01.T01:CDS | 55.0% |
| GGTGACGCCCTTCACGTAGG+AGG | - | Chr7:74674870-74674889 | MsG0780040231.01.T01:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr7 | gene | 74674846 | 74675256 | 74674846 | ID=MsG0780040231.01;Name=MsG0780040231.01 |
| Chr7 | mRNA | 74674846 | 74675256 | 74674846 | ID=MsG0780040231.01.T01;Parent=MsG0780040231.01;Name=MsG0780040231.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|136 |
| Chr7 | exon | 74674846 | 74675256 | 74674846 | ID=MsG0780040231.01.T01:exon:14665;Parent=MsG0780040231.01.T01 |
| Chr7 | CDS | 74674846 | 74675256 | 74674846 | ID=MsG0780040231.01.T01:cds;Parent=MsG0780040231.01.T01 |
| Gene Sequence |
| Protein sequence |
