Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780040555.01.T01 | AES81421.1 | 97.753 | 89 | 2 | 0 | 1 | 89 | 1 | 89 | 2.29E-55 | 176 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780040555.01.T01 | G7KUG1 | 97.753 | 89 | 2 | 0 | 1 | 89 | 1 | 89 | 1.09e-55 | 176 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0780040555.01 | MsG0880045503.01 | 0.800453 | 1.431337e-48 | 6.667088e-46 |
| MsG0380017394.01 | MsG0780040555.01 | 0.849188 | 3.795075e-60 | 7.138025e-57 |
| MsG0280009442.01 | MsG0780040555.01 | 0.876128 | 1.785270e-68 | 8.556109e-65 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780040555.01.T01 | MTR_7g092580 | 97.753 | 89 | 2 | 0 | 1 | 89 | 1 | 89 | 2.78e-59 | 176 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 20 sgRNAs with CRISPR-Local
Find 19 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TATAAACCAAGAGGTATTAT+TGG | 0.269873 | 7:+79151562 | None:intergenic |
| AACGTGACTTTCTACAAATA+AGG | 0.390928 | 7:+79151696 | None:intergenic |
| GGCACAATGTTGGGATCAAT+TGG | 0.419457 | 7:-79151590 | MsG0780040555.01.T01:CDS |
| ATAAACCAAGAGGTATTATT+GGG | 0.432976 | 7:+79151563 | None:intergenic |
| TTGAGTAACATTCAGTCCTT+CGG | 0.448913 | 7:-79151646 | MsG0780040555.01.T01:CDS |
| GTTATCACTGGCACAATGTT+GGG | 0.449308 | 7:-79151599 | MsG0780040555.01.T01:CDS |
| AATGCAAGCATCTGAGTTGT+TGG | 0.458760 | 7:+79151529 | None:intergenic |
| GTCAGCCCAATAATACCTCT+TGG | 0.465872 | 7:-79151568 | MsG0780040555.01.T01:CDS |
| TGTTATCACTGGCACAATGT+TGG | 0.476557 | 7:-79151600 | MsG0780040555.01.T01:CDS |
| AGGACTGAATGTTACTCAAA+AGG | 0.483578 | 7:+79151650 | None:intergenic |
| CAACCAATGGAGGTTACCAT+TGG | 0.500117 | 7:+79151766 | None:intergenic |
| AATCCAATGGTAACCTCCAT+TGG | 0.517402 | 7:-79151769 | None:intergenic |
| TAGTAGAAAGCAACCAAAGA+AGG | 0.542165 | 7:+79151722 | None:intergenic |
| ACAAATGTCTATGTTATCAC+TGG | 0.554133 | 7:-79151611 | MsG0780040555.01.T01:CDS |
| ATAGTAATATATGCAACCAA+TGG | 0.575154 | 7:+79151753 | None:intergenic |
| TTGTCTATTTATAGTACCGA+AGG | 0.576385 | 7:+79151630 | None:intergenic |
| GTCACGTTGTGCTTGTACGT+GGG | 0.604339 | 7:-79151681 | MsG0780040555.01.T01:CDS |
| AGTCACGTTGTGCTTGTACG+TGG | 0.607218 | 7:-79151682 | MsG0780040555.01.T01:CDS |
| GTAATATATGCAACCAATGG+AGG | 0.627637 | 7:+79151756 | None:intergenic |
| AAGTTTGTATATAAACCAAG+AGG | 0.715622 | 7:+79151553 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AAGTTTGTATATAAACCAAG+AGG | + | Chr7:79151729-79151748 | None:intergenic | 25.0% |
| ! | ATAGTAATATATGCAACCAA+TGG | + | Chr7:79151529-79151548 | None:intergenic | 25.0% |
| !!! | ATAAACCAAGAGGTATTATT+GGG | + | Chr7:79151719-79151738 | None:intergenic | 25.0% |
| !!! | TATAAACCAAGAGGTATTAT+TGG | + | Chr7:79151720-79151739 | None:intergenic | 25.0% |
| AACGTGACTTTCTACAAATA+AGG | + | Chr7:79151586-79151605 | None:intergenic | 30.0% | |
| ACAAATGTCTATGTTATCAC+TGG | - | Chr7:79151668-79151687 | MsG0780040555.01.T01:CDS | 30.0% | |
| ! | ACTATCTTTTGTTCCTTCTT+TGG | - | Chr7:79151544-79151563 | MsG0780040555.01.T01:CDS | 30.0% |
| ! | TTGTCTATTTATAGTACCGA+AGG | + | Chr7:79151652-79151671 | None:intergenic | 30.0% |
| AGGACTGAATGTTACTCAAA+AGG | + | Chr7:79151632-79151651 | None:intergenic | 35.0% | |
| GTAATATATGCAACCAATGG+AGG | + | Chr7:79151526-79151545 | None:intergenic | 35.0% | |
| TAGTAGAAAGCAACCAAAGA+AGG | + | Chr7:79151560-79151579 | None:intergenic | 35.0% | |
| TTGAGTAACATTCAGTCCTT+CGG | - | Chr7:79151633-79151652 | MsG0780040555.01.T01:CDS | 35.0% | |
| AATGCAAGCATCTGAGTTGT+TGG | + | Chr7:79151753-79151772 | None:intergenic | 40.0% | |
| GTTATCACTGGCACAATGTT+GGG | - | Chr7:79151680-79151699 | MsG0780040555.01.T01:CDS | 40.0% | |
| TGTTATCACTGGCACAATGT+TGG | - | Chr7:79151679-79151698 | MsG0780040555.01.T01:CDS | 40.0% | |
| GGCACAATGTTGGGATCAAT+TGG | - | Chr7:79151689-79151708 | MsG0780040555.01.T01:CDS | 45.0% | |
| GTCAGCCCAATAATACCTCT+TGG | - | Chr7:79151711-79151730 | MsG0780040555.01.T01:CDS | 45.0% | |
| AGTCACGTTGTGCTTGTACG+TGG | - | Chr7:79151597-79151616 | MsG0780040555.01.T01:CDS | 50.0% | |
| GTCACGTTGTGCTTGTACGT+GGG | - | Chr7:79151598-79151617 | MsG0780040555.01.T01:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr7 | gene | 79151516 | 79151785 | 79151516 | ID=MsG0780040555.01;Name=MsG0780040555.01 |
| Chr7 | mRNA | 79151516 | 79151785 | 79151516 | ID=MsG0780040555.01.T01;Parent=MsG0780040555.01;Name=MsG0780040555.01.T01;_AED=0.32;_eAED=0.32;_QI=0|-1|0|1|-1|0|1|0|89 |
| Chr7 | exon | 79151516 | 79151785 | 79151516 | ID=MsG0780040555.01.T01:exon:441;Parent=MsG0780040555.01.T01 |
| Chr7 | CDS | 79151516 | 79151785 | 79151516 | ID=MsG0780040555.01.T01:cds;Parent=MsG0780040555.01.T01 |
| Gene Sequence |
| Protein sequence |
