Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380017394.01.T01 | XP_013461880.1 | 98.104 | 211 | 4 | 0 | 1 | 211 | 1 | 211 | 5.75E-150 | 425 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380017394.01.T01 | P32765 | 35.238 | 210 | 109 | 11 | 6 | 198 | 11 | 210 | 5.80E-15 | 73.9 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380017394.01.T01 | A0A072VCP7 | 98.104 | 211 | 4 | 0 | 1 | 211 | 1 | 211 | 2.75e-150 | 425 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0380016316.01 | MsG0380017394.01 | 0.808065 | 3.718475e-50 | 2.105358e-47 |
| MsG0380017394.01 | MsG0580024798.01 | 0.818128 | 2.303907e-52 | 1.709868e-49 |
| MsG0380017394.01 | MsG0680034239.01 | 0.890595 | 8.548450e-74 | 7.235668e-70 |
| MsG0380017394.01 | MsG0680034415.01 | 0.838981 | 2.081640e-57 | 2.834661e-54 |
| MsG0380017394.01 | MsG0780040139.01 | 0.814120 | 1.810958e-51 | 1.204692e-48 |
| MsG0380017394.01 | MsG0780040555.01 | 0.849188 | 3.795075e-60 | 7.138025e-57 |
| MsG0380017394.01 | MsG0880042433.01 | 0.804560 | 2.037028e-49 | 1.053598e-46 |
| MsG0380017394.01 | MsG0880045503.01 | 0.814336 | 1.622598e-51 | 1.085762e-48 |
| MsG0280009442.01 | MsG0380017394.01 | 0.812547 | 4.013030e-51 | 2.559022e-48 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380017394.01.T01 | MTR_3g106585 | 98.104 | 211 | 4 | 0 | 1 | 211 | 1 | 211 | 6.97e-154 | 425 |
| MsG0380017394.01.T01 | MTR_3g106620 | 81.221 | 213 | 38 | 1 | 1 | 211 | 1 | 213 | 1.04e-125 | 353 |
| MsG0380017394.01.T01 | MTR_6g078070 | 34.555 | 191 | 100 | 8 | 24 | 197 | 21 | 203 | 2.76e-22 | 90.1 |
| MsG0380017394.01.T01 | MTR_6g078290 | 33.333 | 216 | 109 | 10 | 9 | 198 | 7 | 213 | 2.20e-21 | 88.2 |
| MsG0380017394.01.T01 | MTR_6g078100 | 34.197 | 193 | 100 | 8 | 24 | 197 | 21 | 205 | 2.71e-20 | 85.1 |
| MsG0380017394.01.T01 | MTR_6g078120 | 31.579 | 209 | 104 | 10 | 17 | 197 | 16 | 213 | 2.61e-15 | 72.0 |
| MsG0380017394.01.T01 | MTR_7g108815 | 30.000 | 180 | 112 | 8 | 27 | 196 | 25 | 200 | 2.82e-13 | 66.2 |
| MsG0380017394.01.T01 | MTR_6g009563 | 31.953 | 169 | 99 | 7 | 4 | 162 | 7 | 169 | 1.49e-12 | 64.3 |
| MsG0380017394.01.T01 | MTR_6g017225 | 38.043 | 92 | 53 | 2 | 12 | 99 | 13 | 104 | 4.14e-12 | 64.7 |
| MsG0380017394.01.T01 | MTR_6g009650 | 31.325 | 166 | 95 | 7 | 6 | 162 | 13 | 168 | 5.62e-12 | 62.8 |
| MsG0380017394.01.T01 | MTR_6g059650 | 29.570 | 186 | 109 | 8 | 27 | 198 | 26 | 203 | 5.93e-12 | 62.8 |
| MsG0380017394.01.T01 | MTR_6g078280 | 28.571 | 203 | 114 | 10 | 19 | 197 | 17 | 212 | 9.73e-11 | 59.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380017394.01.T01 | AT1G73260 | 33.871 | 186 | 100 | 11 | 28 | 198 | 29 | 206 | 3.23e-15 | 71.6 |
| MsG0380017394.01.T01 | AT1G17860 | 32.857 | 210 | 104 | 14 | 6 | 197 | 5 | 195 | 4.15e-15 | 71.2 |
| MsG0380017394.01.T01 | AT1G73330 | 31.694 | 183 | 108 | 10 | 24 | 199 | 22 | 194 | 2.09e-14 | 69.3 |
| MsG0380017394.01.T01 | AT1G73325 | 28.426 | 197 | 123 | 7 | 12 | 197 | 14 | 203 | 4.17e-12 | 63.5 |
Find 49 sgRNAs with CRISPR-Local
Find 57 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CACCATTGAGATTCCAATTC+TGG | 0.176056 | 3:-95767372 | MsG0380017394.01.T01:CDS |
| AAGTTCTGTCCTTCTGTTCC+TGG | 0.220195 | 3:-95767197 | MsG0380017394.01.T01:CDS |
| TAAAGGTTGGATCTGGGTTT+TGG | 0.257005 | 3:-95767313 | MsG0380017394.01.T01:CDS |
| TTTAAAAGTCTCCACACTTT+TGG | 0.285295 | 3:+95767332 | None:intergenic |
| GTGAGCACTGGTGGTGCAGC+TGG | 0.296941 | 3:-95767287 | MsG0380017394.01.T01:CDS |
| TTCAAGATTGAGAGGCTTGC+AGG | 0.297937 | 3:-95767248 | MsG0380017394.01.T01:CDS |
| TTATTATGTATTTCCACTTT+GGG | 0.342016 | 3:-95767534 | MsG0380017394.01.T01:CDS |
| TTGCAAGGACTGCCCAGAAT+TGG | 0.350605 | 3:+95767359 | None:intergenic |
| CCAGTTTATTTCTCTGCATC+AGG | 0.372005 | 3:-95767425 | MsG0380017394.01.T01:CDS |
| GTTATTATGTATTTCCACTT+TGG | 0.390718 | 3:-95767535 | MsG0380017394.01.T01:CDS |
| ACTGGTGTGCCAATTGCATC+AGG | 0.400173 | 3:+95767443 | None:intergenic |
| TAATGTTATGCCTCCATTGT+CGG | 0.403372 | 3:+95767511 | None:intergenic |
| TGCCAATTGCATCAGGGTTA+CGG | 0.410323 | 3:+95767450 | None:intergenic |
| ACCATTGAGATTCCAATTCT+GGG | 0.428272 | 3:-95767371 | MsG0380017394.01.T01:CDS |
| CCTGATGCAGAGAAATAAAC+TGG | 0.432071 | 3:+95767425 | None:intergenic |
| TTCTGAAATCTAACATAGTA+AGG | 0.445442 | 3:+95767095 | None:intergenic |
| TGTTTGTCTACTACTGCCTC+TGG | 0.457272 | 3:+95767584 | None:intergenic |
| ATCCGTAACCCTGATGCAAT+TGG | 0.467790 | 3:-95767452 | MsG0380017394.01.T01:CDS |
| ATTATTGCTCTGAGCAAAAG+TGG | 0.480580 | 3:+95767067 | None:intergenic |
| TGAGCACTGGTGGTGCAGCT+GGG | 0.484946 | 3:-95767286 | MsG0380017394.01.T01:CDS |
| CAAAGAACACCAGGAACAGA+AGG | 0.492794 | 3:+95767188 | None:intergenic |
| GACAATGGAGGCATAACATT+AGG | 0.494830 | 3:-95767509 | MsG0380017394.01.T01:CDS |
| TACTGATGGAACTAAAGTTA+TGG | 0.520354 | 3:-95767141 | MsG0380017394.01.T01:CDS |
| GGTGTTCTTTGTGCTCCTGT+AGG | 0.523697 | 3:-95767176 | MsG0380017394.01.T01:CDS |
| TCTGGTTCAGCTCCTTGTAG+TGG | 0.532299 | 3:+95767602 | None:intergenic |
| TTACTATGTTAGATTTCAGA+AGG | 0.535320 | 3:-95767093 | MsG0380017394.01.T01:CDS |
| GTATCTACAAAAGTTCCTAC+AGG | 0.540405 | 3:+95767161 | None:intergenic |
| GAGCACTGGTGGTGCAGCTG+GGG | 0.547443 | 3:-95767285 | MsG0380017394.01.T01:CDS |
| TGAACAACGCTAAGAGAGAA+AGG | 0.548980 | 3:+95767633 | None:intergenic |
| CCACTTTGGGCCGACAATGG+AGG | 0.549557 | 3:-95767521 | MsG0380017394.01.T01:CDS |
| AACCTTCACCTGGTTGAAGA+GGG | 0.550922 | 3:+95767555 | None:intergenic |
| ATGGTGAGATCAGTGAGAGT+TGG | 0.553098 | 3:+95767389 | None:intergenic |
| TAACCTTCACCTGGTTGAAG+AGG | 0.554679 | 3:+95767554 | None:intergenic |
| TTTCCACTTTGGGCCGACAA+TGG | 0.555677 | 3:-95767524 | MsG0380017394.01.T01:CDS |
| GCCCAGAATTGGAATCTCAA+TGG | 0.560561 | 3:+95767370 | None:intergenic |
| AAAGTTATGGCTGTTGGCGA+TGG | 0.567290 | 3:-95767128 | MsG0380017394.01.T01:CDS |
| TTAGCAAGTTCAAGATTGAG+AGG | 0.571309 | 3:-95767256 | MsG0380017394.01.T01:CDS |
| TTCAACACAAAGCCACTACA+AGG | 0.573110 | 3:-95767614 | MsG0380017394.01.T01:CDS |
| CCTCCATTGTCGGCCCAAAG+TGG | 0.575834 | 3:+95767521 | None:intergenic |
| AACCCTCTTCAACCAGGTGA+AGG | 0.586328 | 3:-95767557 | MsG0380017394.01.T01:CDS |
| CAAGGTAACCCTCTTCAACC+AGG | 0.592964 | 3:-95767563 | MsG0380017394.01.T01:CDS |
| GGAACTAAAGTTATGGCTGT+TGG | 0.595929 | 3:-95767134 | MsG0380017394.01.T01:CDS |
| ACAGGAGCACAAAGAACACC+AGG | 0.637032 | 3:+95767179 | None:intergenic |
| CTGGTGTGCCAATTGCATCA+GGG | 0.645208 | 3:+95767444 | None:intergenic |
| CTTGCAATGAACCAAAAGTG+TGG | 0.645641 | 3:-95767343 | MsG0380017394.01.T01:CDS |
| ACTACAAGGAGCTGAACCAG+AGG | 0.651073 | 3:-95767600 | MsG0380017394.01.T01:CDS |
| GGGTTACGGATTACGTCAAG+AGG | 0.654762 | 3:+95767464 | None:intergenic |
| AATACATAATAACCTTCACC+TGG | 0.663956 | 3:+95767545 | None:intergenic |
| GAGGCAGTAGTAGACAAACA+AGG | 0.729599 | 3:-95767581 | MsG0380017394.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTATTATGTATTTCCACTTT+GGG | - | Chr3:95767171-95767190 | MsG0380017394.01.T01:CDS | 20.0% |
| ! | GTTATTATGTATTTCCACTT+TGG | - | Chr3:95767170-95767189 | MsG0380017394.01.T01:CDS | 25.0% |
| ! | TTACTATGTTAGATTTCAGA+AGG | - | Chr3:95767612-95767631 | MsG0380017394.01.T01:CDS | 25.0% |
| !!! | TTCTGAAATCTAACATAGTA+AGG | + | Chr3:95767613-95767632 | None:intergenic | 25.0% |
| AATACATAATAACCTTCACC+TGG | + | Chr3:95767163-95767182 | None:intergenic | 30.0% | |
| TACTGATGGAACTAAAGTTA+TGG | - | Chr3:95767564-95767583 | MsG0380017394.01.T01:CDS | 30.0% | |
| ! | TTTAAAAGTCTCCACACTTT+TGG | + | Chr3:95767376-95767395 | None:intergenic | 30.0% |
| !! | AAAAGTGTGGAGACTTTTAA+AGG | - | Chr3:95767375-95767394 | MsG0380017394.01.T01:CDS | 30.0% |
| ACCATTGAGATTCCAATTCT+GGG | - | Chr3:95767334-95767353 | MsG0380017394.01.T01:CDS | 35.0% | |
| GTATCTACAAAAGTTCCTAC+AGG | + | Chr3:95767547-95767566 | None:intergenic | 35.0% | |
| TAATGTTATGCCTCCATTGT+CGG | + | Chr3:95767197-95767216 | None:intergenic | 35.0% | |
| TTAGCAAGTTCAAGATTGAG+AGG | - | Chr3:95767449-95767468 | MsG0380017394.01.T01:CDS | 35.0% | |
| ! | ATTATTGCTCTGAGCAAAAG+TGG | + | Chr3:95767641-95767660 | None:intergenic | 35.0% |
| ! | GGAACTTTTGTAGATACTGA+TGG | - | Chr3:95767550-95767569 | MsG0380017394.01.T01:CDS | 35.0% |
| !! | AGACTTTTAAAGGTTGGATC+TGG | - | Chr3:95767385-95767404 | MsG0380017394.01.T01:CDS | 35.0% |
| !! | GACTTTTAAAGGTTGGATCT+GGG | - | Chr3:95767386-95767405 | MsG0380017394.01.T01:CDS | 35.0% |
| CACCATTGAGATTCCAATTC+TGG | - | Chr3:95767333-95767352 | MsG0380017394.01.T01:CDS | 40.0% | |
| CCTGATGCAGAGAAATAAAC+TGG | + | Chr3:95767283-95767302 | None:intergenic | 40.0% | |
| CTTGCAATGAACCAAAAGTG+TGG | - | Chr3:95767362-95767381 | MsG0380017394.01.T01:CDS | 40.0% | |
| GGAACTAAAGTTATGGCTGT+TGG | - | Chr3:95767571-95767590 | MsG0380017394.01.T01:CDS | 40.0% | |
| TGAACAACGCTAAGAGAGAA+AGG | + | Chr3:95767075-95767094 | None:intergenic | 40.0% | |
| TTCAACACAAAGCCACTACA+AGG | - | Chr3:95767091-95767110 | MsG0380017394.01.T01:CDS | 40.0% | |
| ! | CCAGTTTATTTCTCTGCATC+AGG | - | Chr3:95767280-95767299 | MsG0380017394.01.T01:CDS | 40.0% |
| ! | GACAATGGAGGCATAACATT+AGG | - | Chr3:95767196-95767215 | MsG0380017394.01.T01:CDS | 40.0% |
| ! | TAAAGGTTGGATCTGGGTTT+TGG | - | Chr3:95767392-95767411 | MsG0380017394.01.T01:CDS | 40.0% |
| !! | GTGTGGAGACTTTTAAAGGT+TGG | - | Chr3:95767379-95767398 | MsG0380017394.01.T01:CDS | 40.0% |
| !!! | CACACTTTTGGTTCATTGCA+AGG | + | Chr3:95767364-95767383 | None:intergenic | 40.0% |
| AAAGTTATGGCTGTTGGCGA+TGG | - | Chr3:95767577-95767596 | MsG0380017394.01.T01:CDS | 45.0% | |
| AACCTTCACCTGGTTGAAGA+GGG | + | Chr3:95767153-95767172 | None:intergenic | 45.0% | |
| AAGTTCTGTCCTTCTGTTCC+TGG | - | Chr3:95767508-95767527 | MsG0380017394.01.T01:CDS | 45.0% | |
| ATCCGTAACCCTGATGCAAT+TGG | - | Chr3:95767253-95767272 | MsG0380017394.01.T01:CDS | 45.0% | |
| ATGGTGAGATCAGTGAGAGT+TGG | + | Chr3:95767319-95767338 | None:intergenic | 45.0% | |
| CAAAGAACACCAGGAACAGA+AGG | + | Chr3:95767520-95767539 | None:intergenic | 45.0% | |
| GAGGCAGTAGTAGACAAACA+AGG | - | Chr3:95767124-95767143 | MsG0380017394.01.T01:CDS | 45.0% | |
| GCCCAGAATTGGAATCTCAA+TGG | + | Chr3:95767338-95767357 | None:intergenic | 45.0% | |
| TAACCTTCACCTGGTTGAAG+AGG | + | Chr3:95767154-95767173 | None:intergenic | 45.0% | |
| TGCCAATTGCATCAGGGTTA+CGG | + | Chr3:95767258-95767277 | None:intergenic | 45.0% | |
| TGTTTGTCTACTACTGCCTC+TGG | + | Chr3:95767124-95767143 | None:intergenic | 45.0% | |
| TTCAAGATTGAGAGGCTTGC+AGG | - | Chr3:95767457-95767476 | MsG0380017394.01.T01:CDS | 45.0% | |
| !!! | TTTTGGTTTGTGAGCACTGG+TGG | - | Chr3:95767409-95767428 | MsG0380017394.01.T01:CDS | 45.0% |
| AACCCTCTTCAACCAGGTGA+AGG | - | Chr3:95767148-95767167 | MsG0380017394.01.T01:CDS | 50.0% | |
| ACTACAAGGAGCTGAACCAG+AGG | - | Chr3:95767105-95767124 | MsG0380017394.01.T01:CDS | 50.0% | |
| ACTGGTGTGCCAATTGCATC+AGG | + | Chr3:95767265-95767284 | None:intergenic | 50.0% | |
| CAAGGTAACCCTCTTCAACC+AGG | - | Chr3:95767142-95767161 | MsG0380017394.01.T01:CDS | 50.0% | |
| CTGGTGTGCCAATTGCATCA+GGG | + | Chr3:95767264-95767283 | None:intergenic | 50.0% | |
| GGTGTTCTTTGTGCTCCTGT+AGG | - | Chr3:95767529-95767548 | MsG0380017394.01.T01:CDS | 50.0% | |
| TTGCAAGGACTGCCCAGAAT+TGG | + | Chr3:95767349-95767368 | None:intergenic | 50.0% | |
| TTTCCACTTTGGGCCGACAA+TGG | - | Chr3:95767181-95767200 | MsG0380017394.01.T01:CDS | 50.0% | |
| ! | ACAGGAGCACAAAGAACACC+AGG | + | Chr3:95767529-95767548 | None:intergenic | 50.0% |
| ! | GGGTTACGGATTACGTCAAG+AGG | + | Chr3:95767244-95767263 | None:intergenic | 50.0% |
| ! | TCTGGTTCAGCTCCTTGTAG+TGG | + | Chr3:95767106-95767125 | None:intergenic | 50.0% |
| !!! | GGGTTTTGGTTTGTGAGCAC+TGG | - | Chr3:95767406-95767425 | MsG0380017394.01.T01:CDS | 50.0% |
| CCACTTTGGGCCGACAATGG+AGG | - | Chr3:95767184-95767203 | MsG0380017394.01.T01:CDS | 60.0% | |
| CCTCCATTGTCGGCCCAAAG+TGG | + | Chr3:95767187-95767206 | None:intergenic | 60.0% | |
| !! | TGAGCACTGGTGGTGCAGCT+GGG | - | Chr3:95767419-95767438 | MsG0380017394.01.T01:CDS | 60.0% |
| !! | GAGCACTGGTGGTGCAGCTG+GGG | - | Chr3:95767420-95767439 | MsG0380017394.01.T01:CDS | 65.0% |
| !! | GTGAGCACTGGTGGTGCAGC+TGG | - | Chr3:95767418-95767437 | MsG0380017394.01.T01:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr3 | gene | 95767046 | 95767681 | 95767046 | ID=MsG0380017394.01;Name=MsG0380017394.01 |
| Chr3 | mRNA | 95767046 | 95767681 | 95767046 | ID=MsG0380017394.01.T01;Parent=MsG0380017394.01;Name=MsG0380017394.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|211 |
| Chr3 | exon | 95767046 | 95767681 | 95767046 | ID=MsG0380017394.01.T01:exon:4816;Parent=MsG0380017394.01.T01 |
| Chr3 | CDS | 95767046 | 95767681 | 95767046 | ID=MsG0380017394.01.T01:cds;Parent=MsG0380017394.01.T01 |
| Gene Sequence |
| Protein sequence |
