AlfalfaGEDB | MsG0880045503.01

M. sativa cultivar ZhongmuNo.1 / MsG0880045503.01

KEGG: K00021
GO: GO:0004420 GO:0015936 GO:0016616 GO:0050662 GO:0055114
PFAM: PF00368

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880045503.01.T01	XP_003629056.1	74.675	154	9	2	1	124	83	236	5.91E-62	215
MsG0880045503.01.T01	XP_003629056.1	84	100	10	1	168	267	490	583	1.96E-39	155
MsG0880045503.01.T01	XP_003629056.1	67.593	108	27	2	109	211	284	388	4.24E-34	140

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880045503.01.T01	O24594	38.182	275	81	10	1	188	76	348	3.00E-41	152

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880045503.01.T01	A9YVC5	74.675	154	9	2	1	124	83	236	2.82e-62	215

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080048509.01	MsG0880045503.01	0.819798	9.611652e-53	7.471634e-50
MsG0180000076.01	MsG0880045503.01	0.803167	3.966768e-49	1.979581e-46
MsG0180001290.01	MsG0880045503.01	0.802143	6.453156e-49	3.137339e-46
MsG0180001358.01	MsG0880045503.01	0.808930	2.431494e-50	1.408258e-47
MsG0880044688.01	MsG0880045503.01	0.851058	1.136366e-60	2.271608e-57
MsG0880044984.01	MsG0880045503.01	0.812773	3.581522e-51	2.297251e-48
MsG0880045233.01	MsG0880045503.01	0.801424	9.063858e-49	4.326969e-46
MsG0880045439.01	MsG0880045503.01	0.810040	1.404850e-50	8.380579e-48
MsG0880045503.01	MsG0880045584.01	0.839276	1.745325e-57	2.398227e-54
MsG0880045503.01	MsG0880045612.01	0.804268	2.344145e-49	1.203326e-46
MsG0880045503.01	MsG0880045647.01	0.813831	2.097389e-51	1.384330e-48
MsG0880045503.01	MsG0880045703.01	-0.800426	1.449034e-48	6.745320e-46
MsG0880045503.01	MsG0880045802.01	0.876761	1.079973e-68	5.298794e-65
MsG0880045503.01	MsG0880045852.01	0.849917	2.376594e-60	4.576955e-57
MsG0880045503.01	MsG0880045856.01	0.832436	9.465100e-56	1.057869e-52
MsG0880045503.01	MsG0880046137.01	0.839692	1.361017e-57	1.894119e-54
MsG0880045503.01	MsG0880046693.01	0.815006	1.153158e-51	7.857331e-49
MsG0880045503.01	MsG0880046945.01	0.828455	8.903224e-55	8.854233e-52
MsG0880045503.01	MsG0880047057.01	0.815682	8.156980e-52	5.661739e-49
MsG0880045503.01	MsG0880047649.01	0.810945	8.956949e-51	5.473224e-48
MsG0180002808.01	MsG0880045503.01	0.802808	4.705110e-49	2.326801e-46
MsG0180004559.01	MsG0880045503.01	0.808264	3.373574e-50	1.920221e-47
MsG0180004652.01	MsG0880045503.01	0.809866	1.530967e-50	9.091292e-48
MsG0180004705.01	MsG0880045503.01	0.818445	1.953399e-52	1.462642e-49
MsG0180005096.01	MsG0880045503.01	0.815308	9.879681e-52	6.787270e-49
MsG0180005325.01	MsG0880045503.01	0.804711	1.894573e-49	9.837729e-47
MsG0180006026.01	MsG0880045503.01	0.818167	2.257609e-52	1.677276e-49
MsG0180006027.01	MsG0880045503.01	0.826862	2.148493e-54	2.039452e-51
MsG0180006076.01	MsG0880045503.01	-0.802940	4.418906e-49	2.192403e-46
MsG0180006182.01	MsG0880045503.01	0.845540	3.811441e-59	6.372579e-56
MsG0180006191.01	MsG0880045503.01	0.851298	9.721548e-61	1.958964e-57
MsG0280006426.01	MsG0880045503.01	0.834848	2.364425e-56	2.840449e-53
MsG0780040555.01	MsG0880045503.01	0.800453	1.431337e-48	6.667088e-46
MsG0780040560.01	MsG0880045503.01	0.803042	4.209994e-49	2.094160e-46
MsG0780041100.01	MsG0880045503.01	0.837883	3.996264e-57	5.262876e-54
MsG0780041180.01	MsG0880045503.01	0.816770	4.658411e-52	3.331015e-49
MsG0880042541.01	MsG0880045503.01	0.810900	9.157407e-51	5.588853e-48
MsG0880042633.01	MsG0880045503.01	0.809633	1.718168e-50	1.014112e-47
MsG0880042635.01	MsG0880045503.01	0.849823	2.525234e-60	4.847599e-57
MsG0280010617.01	MsG0880045503.01	0.814035	1.891292e-51	1.255236e-48
MsG0380013041.01	MsG0880045503.01	0.882165	1.309760e-70	7.893363e-67
MsG0380013414.01	MsG0880045503.01	0.858147	1.006017e-62	2.542559e-59
MsG0380013415.01	MsG0880045503.01	0.846070	2.735330e-59	4.652335e-56
MsG0380014544.01	MsG0880045503.01	0.811527	6.695046e-51	4.154198e-48
MsG0680034415.01	MsG0880045503.01	0.898272	6.241229e-77	7.323023e-73
MsG0680034417.01	MsG0880045503.01	0.803650	3.150140e-49	1.591628e-46
MsG0480018767.01	MsG0880045503.01	0.829841	4.107235e-55	4.254092e-52
MsG0480019935.01	MsG0880045503.01	0.807915	4.002587e-50	2.257139e-47
MsG0480021773.01	MsG0880045503.01	0.802535	5.358895e-49	2.631469e-46
MsG0480022242.01	MsG0880045503.01	-0.808406	3.145566e-50	1.797117e-47
MsG0480023322.01	MsG0880045503.01	0.814637	1.392141e-51	9.391664e-49
MsG0480023532.01	MsG0880045503.01	0.800617	1.325144e-48	6.198318e-46
MsG0480023944.01	MsG0880045503.01	0.805371	1.378698e-49	7.281466e-47
MsG0580024431.01	MsG0880045503.01	0.858783	6.497301e-63	1.677686e-59
MsG0580025056.01	MsG0880045503.01	0.811899	5.558103e-51	3.482600e-48
MsG0580025464.01	MsG0880045503.01	0.832030	1.192530e-55	1.317008e-52
MsG0380015656.01	MsG0880045503.01	0.807574	4.729941e-50	2.643668e-47
MsG0380015858.01	MsG0880045503.01	0.824378	8.340205e-54	7.374358e-51
MsG0380015871.01	MsG0880045503.01	0.809458	1.873538e-50	1.100581e-47
MsG0380015954.01	MsG0880045503.01	0.824913	6.238489e-54	5.602209e-51
MsG0380016110.01	MsG0880045503.01	0.831961	1.240098e-55	1.366828e-52
MsG0380016316.01	MsG0880045503.01	0.801604	8.325438e-49	3.992397e-46
MsG0380017394.01	MsG0880045503.01	0.814336	1.622598e-51	1.085762e-48
MsG0380017898.01	MsG0880045503.01	0.854911	8.979773e-62	2.037031e-58
MsG0380017989.01	MsG0880045503.01	0.846525	2.056777e-59	3.550296e-56
MsG0380017992.01	MsG0880045503.01	0.811048	8.506220e-51	5.211715e-48
MsG0480018228.01	MsG0880045503.01	0.827990	1.152785e-54	1.131024e-51
MsG0580026311.01	MsG0880045503.01	0.838167	3.377798e-57	4.486595e-54
MsG0580029696.01	MsG0880045503.01	0.809564	1.778060e-50	1.047470e-47
MsG0580029751.01	MsG0880045503.01	-0.812622	3.863998e-51	2.468772e-48
MsG0580030141.01	MsG0880045503.01	0.806780	6.964680e-50	3.813476e-47
MsG0580030179.01	MsG0880045503.01	0.832226	1.067023e-55	1.185091e-52
MsG0280006851.01	MsG0880045503.01	0.820378	7.080747e-53	5.593693e-50
MsG0280007280.01	MsG0880045503.01	0.906662	1.168077e-80	1.999273e-76
MsG0280007281.01	MsG0880045503.01	0.968396	1.282967e-128	1.070179e-122
MsG0280007419.01	MsG0880045503.01	0.802701	4.951432e-49	2.441816e-46
MsG0280007667.01	MsG0880045503.01	0.823482	1.353025e-53	1.166425e-50
MsG0280007901.01	MsG0880045503.01	0.850591	1.538106e-60	3.027973e-57
MsG0280008407.01	MsG0880045503.01	0.818491	1.906822e-52	1.429431e-49
MsG0280008692.01	MsG0880045503.01	0.849006	4.264236e-60	7.972391e-57
MsG0280010303.01	MsG0880045503.01	0.804112	2.526109e-49	1.291606e-46
MsG0780036890.01	MsG0880045503.01	0.805267	1.449924e-49	7.636943e-47
MsG0780037982.01	MsG0880045503.01	-0.827078	1.908267e-54	1.823034e-51
MsG0780038810.01	MsG0880045503.01	0.850758	1.380375e-60	2.732452e-57
MsG0780039642.01	MsG0880045503.01	0.805305	1.423199e-49	7.503680e-47
MsG0780039916.01	MsG0880045503.01	0.844424	7.625565e-59	1.230558e-55
MsG0780040021.01	MsG0880045503.01	0.818330	2.074140e-52	1.547952e-49
MsG0780040139.01	MsG0880045503.01	0.817460	3.259773e-52	2.375504e-49

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880045503.01.T01	MTR_8g072640	74.675	154	9	2	1	124	83	236	7.15e-66	215
MsG0880045503.01.T01	MTR_8g072640	84.000	100	10	1	168	267	490	583	2.37e-43	155
MsG0880045503.01.T01	MTR_8g072640	67.593	108	27	2	109	211	284	388	5.14e-38	140
MsG0880045503.01.T01	MTR_5g091050	75.000	100	19	1	168	267	452	545	7.53e-39	142
MsG0880045503.01.T01	MTR_5g091050	34.630	257	99	7	1	188	69	325	3.44e-32	124
MsG0880045503.01.T01	MTR_5g024880	75.000	100	19	1	168	267	474	567	8.96e-39	142
MsG0880045503.01.T01	MTR_5g024880	68.750	80	25	0	109	188	268	347	2.19e-30	119
MsG0880045503.01.T01	MTR_5g024880	45.033	151	56	4	1	124	70	220	6.35e-28	112
MsG0880045503.01.T01	MTR_5g026450	87.500	80	10	0	188	267	463	542	1.87e-38	141
MsG0880045503.01.T01	MTR_5g026450	34.231	260	99	6	1	188	63	322	1.40e-32	125
MsG0880045503.01.T01	MTR_5g026460	87.500	80	10	0	188	267	463	542	1.87e-38	141
MsG0880045503.01.T01	MTR_5g026460	34.231	260	99	6	1	188	63	322	1.40e-32	125
MsG0880045503.01.T01	MTR_5g026430	87.500	80	10	0	188	267	463	542	1.87e-38	141
MsG0880045503.01.T01	MTR_5g026430	34.231	260	99	6	1	188	63	322	2.17e-32	124
MsG0880045503.01.T01	MTR_5g026480	87.500	80	10	0	188	267	463	542	1.87e-38	141
MsG0880045503.01.T01	MTR_5g026480	34.231	260	99	6	1	188	63	322	1.40e-32	125
MsG0880045503.01.T01	MTR_5g026500	74.000	100	20	1	168	267	457	550	2.60e-38	141
MsG0880045503.01.T01	MTR_5g026500	33.840	263	99	6	1	188	68	330	2.47e-32	124
MsG0880045503.01.T01	MTR_5g087550	85.000	80	12	0	188	267	486	565	7.19e-37	137
MsG0880045503.01.T01	MTR_5g087550	49.275	138	56	3	1	124	81	218	3.13e-28	113
MsG0880045503.01.T01	MTR_5g087550	77.612	67	15	0	122	188	279	345	4.19e-28	112
MsG0880045503.01.T01	MTR_5g024880	56.481	108	39	2	109	211	268	372	1.71e-30	119
MsG0880045503.01.T01	MTR_5g024880	45.033	151	56	4	1	124	70	220	6.40e-28	112

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880045503.01.T01	AT1G76490	93.421	76	5	0	188	263	557	632	1.14e-37	140
MsG0880045503.01.T01	AT1G76490	48.276	145	63	5	122	256	350	492	2.32e-29	117
MsG0880045503.01.T01	AT1G76490	40.816	147	64	2	1	124	143	289	5.43e-25	104
MsG0880045503.01.T01	AT2G17370	86.667	75	10	0	188	262	480	554	1.66e-34	130
MsG0880045503.01.T01	AT2G17370	45.000	140	61	3	1	124	75	214	5.77e-26	107
MsG0880045503.01.T01	AT2G17370	70.149	67	20	0	122	188	275	341	4.45e-24	101

Find 109 sgRNAs with CRISPR-Local

Find 289 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GCCTCCGCCGTTTACCTTCT+AGG	0.154239	8:-60178153	MsG0880045503.01.T01:CDS
TACCGACATCCAAAGTATTT+TGG	0.172144	8:+60174830	None:intergenic
GAATCTCTATGAAAATTATT+TGG	0.183796	8:+60174803	None:intergenic
TATCATGTATCACACTATTT+TGG	0.217144	8:+60174697	None:intergenic
GCAAGCTCACGAGACGATTT+TGG	0.234160	8:+60175401	None:intergenic
TCAGCGGTGCAAACGATAAC+TGG	0.306795	8:-60177825	MsG0880045503.01.T01:CDS
GGAGTTACCGTTGCCGGTTG+TGG	0.347559	8:+60178005	None:intergenic
GTCTGCTAGTCTGGATTCTA+AGG	0.347797	8:+60177875	None:intergenic
TCTTAATTTACTTGGTGTTA+AGG	0.349199	8:-60175580	MsG0880045503.01.T01:CDS
AGCATTCAGCCTACAATTGC+TGG	0.351295	8:-60176564	MsG0880045503.01.T01:CDS
GAGTTACCGTTGCCGGTTGT+GGG	0.362006	8:+60178006	None:intergenic
CTTAATTTACTTGGTGTTAA+GGG	0.362558	8:-60175579	MsG0880045503.01.T01:CDS
GTTGGTTTCAAAAGGACTAT+TGG	0.364875	8:-60175300	MsG0880045503.01.T01:three_prime_UTR
GACGTATTGCCGCCGCTTTA+CGG	0.366700	8:+60177850	None:intergenic
AAATTCTTGCCAGCAATTGT+AGG	0.370194	8:+60176555	None:intergenic
TTTACGGCAGTCTGCTAGTC+TGG	0.385460	8:+60177866	None:intergenic
CTTCTAGGTTTCTTCGGTAT+CGG	0.393361	8:-60178138	MsG0880045503.01.T01:CDS
ACAATTGGAGTTACCGTTGC+CGG	0.405265	8:+60177999	None:intergenic
ATTCGATTCCGTTGCAGTAC+TGG	0.409002	8:-60176528	MsG0880045503.01.T01:CDS
TGGCACTTTCCAAAATACTT+TGG	0.427516	8:-60174839	MsG0880045503.01.T01:three_prime_UTR
CGGATCACGTACTTCCTTCC+CGG	0.427778	8:-60178118	MsG0880045503.01.T01:CDS
AGCATACAGTTGGTTTCAAA+AGG	0.436550	8:-60175308	MsG0880045503.01.T01:three_prime_UTR
GGCCACCATTGTAGCTGGTT+CGG	0.439947	8:-60175517	MsG0880045503.01.T01:CDS
ATAACTGGAAAATCTCTAGA+AGG	0.440221	8:-60177810	MsG0880045503.01.T01:CDS
CTATTGGCCACCATTGTAGC+TGG	0.447817	8:-60175522	MsG0880045503.01.T01:CDS
TCAGCTTGTCTTAATTTACT+TGG	0.448427	8:-60175588	MsG0880045503.01.T01:CDS
GTTTACCTTCTAGGTTTCTT+CGG	0.453882	8:-60178144	MsG0880045503.01.T01:CDS
AAAACAAGTGGCTAAACCTT+TGG	0.455508	8:-60175100	MsG0880045503.01.T01:three_prime_UTR
ATTAGATAGATAACTAAAAC+CGG	0.469792	8:+60174747	None:intergenic
CTCATGTCTGCAATTGCAGC+TGG	0.470089	8:-60175474	MsG0880045503.01.T01:CDS
GGTTCGATTACGATTCGATT+GGG	0.475251	8:-60177774	MsG0880045503.01.T01:CDS
AAGGACTATTGGTGGTGGCC+AGG	0.478957	8:-60175289	MsG0880045503.01.T01:three_prime_UTR
AGTGCTGCTGGACACGGTCC+TGG	0.480294	8:+60178048	None:intergenic
TTTGTCCTTATGAGAGGCAT+TGG	0.482183	8:+60175344	None:intergenic
GGGGACCAATGCCTCTCATA+AGG	0.484549	8:-60175349	MsG0880045503.01.T01:three_prime_UTR
TCTTCGTCGGAGAGATCGTC+CGG	0.485409	8:+60178099	None:intergenic
AGAAGTGTTGAATTAGAAGA+TGG	0.487882	8:-60175125	MsG0880045503.01.T01:three_prime_UTR
TCAGGTTGGTACTGTGGGAG+GGG	0.488097	8:-60175631	MsG0880045503.01.T01:intron
ATAATTCAGGTTGGTACTGT+GGG	0.489921	8:-60175636	MsG0880045503.01.T01:intron
TGCACCTGGAGATTCTGTGC+TGG	0.497072	8:+60175554	None:intergenic
AGGTGCAAATGCTAGGCTAT+TGG	0.497308	8:-60175538	MsG0880045503.01.T01:CDS
ACCGACGTAAAACCCACAAC+CGG	0.500917	8:-60178018	MsG0880045503.01.T01:CDS
CGATACCGAAGAAACCTAGA+AGG	0.505448	8:+60178139	None:intergenic
GAAGAAACCTAGAAGGTAAA+CGG	0.508337	8:+60178146	None:intergenic
ATCTCTAGAAGGTTTACCGT+TGG	0.513091	8:-60177799	MsG0880045503.01.T01:CDS
TTTAAGGGATATTCTTGTGC+CGG	0.517532	8:-60174766	MsG0880045503.01.T01:three_prime_UTR
CTAGAAGGTTTACCGTTGGA+AGG	0.521102	8:-60177795	MsG0880045503.01.T01:CDS
TCGGTGTCGACAAGTGCTGC+TGG	0.521714	8:+60178036	None:intergenic
GGAATACGACGGAATTGAAC+CGG	0.526078	8:+60177896	None:intergenic
GAGTTGTTAATAATAGTCTC+TGG	0.526987	8:-60174916	MsG0880045503.01.T01:three_prime_UTR
TTCCAAAATACTTTGGATGT+CGG	0.527696	8:-60174832	MsG0880045503.01.T01:three_prime_UTR
CACAACAATAAACTTGTAGA+GGG	0.527980	8:-60175369	MsG0880045503.01.T01:three_prime_UTR
TGCAGTACTGGTGATGCCAT+GGG	0.530758	8:-60176516	MsG0880045503.01.T01:CDS
AATCTCCAGGTGCAAATGCT+AGG	0.530949	8:-60175545	MsG0880045503.01.T01:CDS
ACCTAGAAGGTAAACGGCGG+AGG	0.531795	8:+60178152	None:intergenic
GGCGCCAGCACAGAATCTCC+AGG	0.540230	8:-60175558	MsG0880045503.01.T01:CDS
TGAAATACAACAGATCTAGC+AGG	0.545624	8:-60175431	MsG0880045503.01.T01:CDS
TCGACAAGTGCTGCTGGACA+CGG	0.546888	8:+60178042	None:intergenic
GGTTTCAAAAGGACTATTGG+TGG	0.547730	8:-60175297	MsG0880045503.01.T01:three_prime_UTR
TTCAGGTTGGTACTGTGGGA+GGG	0.548296	8:-60175632	MsG0880045503.01.T01:intron
TTGCAGTACTGGTGATGCCA+TGG	0.550722	8:-60176517	MsG0880045503.01.T01:CDS
AGGTTCGATTACGATTCGAT+TGG	0.559954	8:-60177775	MsG0880045503.01.T01:CDS
AGTGTTGTGAAATGCCGATA+GGG	0.561600	8:-60177749	MsG0880045503.01.T01:intron
ACAGAAAAGGTTGCATCTAG+TGG	0.563519	8:-60175200	MsG0880045503.01.T01:three_prime_UTR
ATTCAGGTTGGTACTGTGGG+AGG	0.563884	8:-60175633	MsG0880045503.01.T01:intron
AATAATTCAGGTTGGTACTG+TGG	0.566379	8:-60175637	MsG0880045503.01.T01:intron
ACACAACAATAAACTTGTAG+AGG	0.567966	8:-60175370	MsG0880045503.01.T01:three_prime_UTR
TGACACCATGTTCATCCCCA+TGG	0.567983	8:+60176500	None:intergenic
TTCAAAAGGACTATTGGTGG+TGG	0.569247	8:-60175294	MsG0880045503.01.T01:three_prime_UTR
CAGGTTGGTACTGTGGGAGG+GGG	0.569796	8:-60175630	MsG0880045503.01.T01:CDS
TTCAACACCTTAGTCCATGT+GGG	0.577948	8:-60175175	MsG0880045503.01.T01:three_prime_UTR
CAGTGTTGTGAAATGCCGAT+AGG	0.585519	8:-60177750	MsG0880045503.01.T01:CDS
ATTGCAAAATCAGTTGTCAC+CGG	0.587890	8:-60177915	MsG0880045503.01.T01:intron
AGCAGACTGCCGTAAAGCGG+CGG	0.591467	8:-60177859	MsG0880045503.01.T01:CDS
GTAGCTGGTTCGGTACTCGC+CGG	0.591964	8:-60175507	MsG0880045503.01.T01:CDS
AAAGAAGACTCTCGAAAACC+AGG	0.593503	8:-60178066	MsG0880045503.01.T01:CDS
TGTTAATAATAGTCTCTGGT+TGG	0.595109	8:-60174912	MsG0880045503.01.T01:three_prime_UTR
GTAAAACCCACAACCGGCAA+CGG	0.595492	8:-60178012	MsG0880045503.01.T01:intron
AAGAATCAAACTAATGCCAA+AGG	0.595697	8:+60175084	None:intergenic
TTGAGCTCAACAAGAGCATG+TGG	0.595929	8:-60175235	MsG0880045503.01.T01:three_prime_UTR
ATCGTAATCGAACCTTCCAA+CGG	0.597607	8:+60177783	None:intergenic
GGCGGCAATACGTCGTTCAG+CGG	0.601502	8:-60177841	MsG0880045503.01.T01:CDS
TTTAGCGAGAATCTCTTCGT+CGG	0.601897	8:+60178086	None:intergenic
GGAATTTGAACAAACCCTAT+CGG	0.606204	8:+60177735	None:intergenic
GCAAGTGTATCAAAATTAAG+CGG	0.609171	8:+60177492	None:intergenic
ACTAGCAGACTGCCGTAAAG+CGG	0.612353	8:-60177862	MsG0880045503.01.T01:CDS
AATAGCCTAGCATTTGCACC+TGG	0.614511	8:+60175540	None:intergenic
GTCGGAGAGATCGTCCGGGA+AGG	0.614794	8:+60178104	None:intergenic
ATTCAACACCTTAGTCCATG+TGG	0.617690	8:-60175176	MsG0880045503.01.T01:three_prime_UTR
TGATGCCATGGGGATGAACA+TGG	0.618362	8:-60176505	MsG0880045503.01.T01:intron
GAGTACCGAACCAGCTACAA+TGG	0.630274	8:+60175512	None:intergenic
GAAATACAACAGATCTAGCA+GGG	0.634441	8:-60175430	MsG0880045503.01.T01:CDS
TGGCATCACCAGTACTGCAA+CGG	0.640383	8:+60176520	None:intergenic
TAGAAGATGGAGAAAACAAG+TGG	0.643097	8:-60175112	MsG0880045503.01.T01:three_prime_UTR
GACTATTGGTGGTGGCCAGG+TGG	0.643231	8:-60175286	MsG0880045503.01.T01:three_prime_UTR
TATAAGAGAAACGATGGCGG+CGG	0.644367	8:+60178176	None:intergenic
TCAACACCTTAGTCCATGTG+GGG	0.654731	8:-60175174	MsG0880045503.01.T01:three_prime_UTR
CTTCGTCGGAGAGATCGTCC+GGG	0.655523	8:+60178100	None:intergenic
GGAGGCTATAAGAGAAACGA+TGG	0.663583	8:+60178170	None:intergenic
TACCGAACCAGCTACAATGG+TGG	0.664745	8:+60175515	None:intergenic
GTTTATTGTTGTGTTGTGTG+AGG	0.668370	8:+60175379	None:intergenic
AGACATGAGTGATAGCTCGC+CGG	0.669906	8:+60175488	None:intergenic
GGCTATAAGAGAAACGATGG+CGG	0.673455	8:+60178173	None:intergenic
AGTGCCAACAGCAAAAGACA+AGG	0.684086	8:+60174855	None:intergenic
CTGGATTCTAAGGAATACGA+CGG	0.686525	8:+60177885	None:intergenic
ACAACAATAAACTTGTAGAG+GGG	0.687899	8:-60175368	MsG0880045503.01.T01:three_prime_UTR
CAACACCTTAGTCCATGTGG+GGG	0.698699	8:-60175173	MsG0880045503.01.T01:three_prime_UTR
GCAGTACTGGTGATGCCATG+GGG	0.721193	8:-60176515	MsG0880045503.01.T01:CDS
GAAACCTAGAAGGTAAACGG+CGG	0.781690	8:+60178149	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TATGTTTATTTAATTCCAAA+GGG	+	Chr8:60176923-60176942	None:intergenic	15.0%
!!	TCATTCATTAACAATTTAAT+GGG	-	Chr8:60176201-60176220	MsG0880045503.01.T01:intron	15.0%
!!	TTATGTTTATTTAATTCCAA+AGG	+	Chr8:60176924-60176943	None:intergenic	15.0%
!!	TTCATTCATTAACAATTTAA+TGG	-	Chr8:60176200-60176219	MsG0880045503.01.T01:intron	15.0%
!!!	TAGATTTTGTTTAATAATTC+AGG	-	Chr8:60177173-60177192	MsG0880045503.01.T01:intron	15.0%
!!!	TCTAAATTTTGTTAAAATCA+CGG	-	Chr8:60175860-60175879	MsG0880045503.01.T01:intron	15.0%
!!!	TTACAATATCTTAAACTTTT+TGG	-	Chr8:60178152-60178171	MsG0880045503.01.T01:CDS	15.0%
!!!	TTTTAACAAAATTTAGACTT+TGG	+	Chr8:60175857-60175876	None:intergenic	15.0%
!!	ATATTTATAAGCAAGTTTAG+GGG	-	Chr8:60177093-60177112	MsG0880045503.01.T01:intron	20.0%
!!	ATTAGATAGATAACTAAAAC+CGG	+	Chr8:60178078-60178097	None:intergenic	20.0%
!!	CATAGATATGAAATGATTTA+GGG	-	Chr8:60175608-60175627	MsG0880045503.01.T01:CDS	20.0%
!!	CATATTTATAAGCAAGTTTA+GGG	-	Chr8:60177092-60177111	MsG0880045503.01.T01:intron	20.0%
!!	GAATCTCTATGAAAATTATT+TGG	+	Chr8:60178022-60178041	None:intergenic	20.0%
!!	TCATAGATATGAAATGATTT+AGG	-	Chr8:60175607-60175626	MsG0880045503.01.T01:CDS	20.0%
!!	TTTAAAAAGAAAATCATGCT+CGG	+	Chr8:60177054-60177073	None:intergenic	20.0%
!!!	AAAAAAAAAGTGTATAGTGA+TGG	+	Chr8:60177936-60177955	None:intergenic	20.0%
!!!	ATAATTTTCATAGAGATTCT+TGG	-	Chr8:60178023-60178042	MsG0880045503.01.T01:CDS	20.0%
!!!	ATCTTAAACTTTTTGGTAAT+GGG	-	Chr8:60178159-60178178	MsG0880045503.01.T01:CDS	20.0%
!!!	CACTAAAATAACAATTTTTG+AGG	+	Chr8:60177821-60177840	None:intergenic	20.0%
!!!	TATCTTAAACTTTTTGGTAA+TGG	-	Chr8:60178158-60178177	MsG0880045503.01.T01:CDS	20.0%
!!!	TTAGTTTTAGATTAACCTAA+AGG	-	Chr8:60176011-60176030	MsG0880045503.01.T01:intron	20.0%
!!!	TTCTGTTTTTAAGAATTACT+GGG	-	Chr8:60176144-60176163	MsG0880045503.01.T01:intron	20.0%
!!!	TTTCTGTTTTTAAGAATTAC+TGG	-	Chr8:60176143-60176162	MsG0880045503.01.T01:intron	20.0%
!!!	TTTTGTTTAATAATTCAGGT+TGG	-	Chr8:60177177-60177196	MsG0880045503.01.T01:intron	20.0%
!	AAACCATTCTATAAACCTTT+AGG	+	Chr8:60176029-60176048	None:intergenic	25.0%
!	ATATCTATGAAGCACAAATA+CGG	+	Chr8:60175597-60175616	None:intergenic	25.0%
!	GCATATTTATAAGCAAGTTT+AGG	-	Chr8:60177091-60177110	MsG0880045503.01.T01:intron	25.0%
!	GTAAGAACAAAAAACAGAAA+AGG	-	Chr8:60177609-60177628	MsG0880045503.01.T01:intron	25.0%
!	TAACCTAAAGGTTTATAGAA+TGG	-	Chr8:60176023-60176042	MsG0880045503.01.T01:intron	25.0%
!	TATTAAACAAAATCTAGCTG+AGG	+	Chr8:60177169-60177188	None:intergenic	25.0%
!!	ACATTTTAAATGTGAACCAA+CGG	+	Chr8:60175710-60175729	None:intergenic	25.0%
!!	GTTTTATCTTGTAGGAAATT+AGG	-	Chr8:60175638-60175657	MsG0880045503.01.T01:intron	25.0%
!!	TATCATGTATCACACTATTT+TGG	+	Chr8:60178128-60178147	None:intergenic	25.0%
!!	TTTTCTACTACATGTATGTT+TGG	+	Chr8:60176999-60177018	None:intergenic	25.0%
!!!	CTTAATTTACTTGGTGTTAA+GGG	-	Chr8:60177243-60177262	MsG0880045503.01.T01:intron	25.0%
!!!	TCTGTTTTTAAGAATTACTG+GGG	-	Chr8:60176145-60176164	MsG0880045503.01.T01:intron	25.0%
!!!	TCTTAAACTTTTTGGTAATG+GGG	-	Chr8:60178160-60178179	MsG0880045503.01.T01:CDS	25.0%
!!!	TCTTAATTTACTTGGTGTTA+AGG	-	Chr8:60177242-60177261	MsG0880045503.01.T01:intron	25.0%
	AAAACAACTGTTTGTCAAAG+AGG	+	Chr8:60175978-60175997	None:intergenic	30.0%
	AAGAAGAGCGAAAATATGTT+TGG	-	Chr8:60175780-60175799	MsG0880045503.01.T01:intron	30.0%
	AAGAATCAAACTAATGCCAA+AGG	+	Chr8:60177741-60177760	None:intergenic	30.0%
	AATGTTTGTCTTTGATCCAA+TGG	-	Chr8:60175725-60175744	MsG0880045503.01.T01:intron	30.0%
	AATTTATGTACCTCAATGGA+AGG	+	Chr8:60176894-60176913	None:intergenic	30.0%
	ACAACAATAAACTTGTAGAG+GGG	-	Chr8:60177454-60177473	MsG0880045503.01.T01:intron	30.0%
	ACACAACAATAAACTTGTAG+AGG	-	Chr8:60177452-60177471	MsG0880045503.01.T01:intron	30.0%
	ATAACTGGAAAATCTCTAGA+AGG	-	Chr8:60175012-60175031	MsG0880045503.01.T01:three_prime_UTR	30.0%
	CACAACAATAAACTTGTAGA+GGG	-	Chr8:60177453-60177472	MsG0880045503.01.T01:intron	30.0%
	CACTAATTTATGTACCTCAA+TGG	+	Chr8:60176898-60176917	None:intergenic	30.0%
	CATGTATGTTTGGCTAAAAA+TGG	+	Chr8:60176989-60177008	None:intergenic	30.0%
	CATTGAGGTACATAAATTAG+TGG	-	Chr8:60176896-60176915	MsG0880045503.01.T01:intron	30.0%
	GAGTTGTTAATAATAGTCTC+TGG	-	Chr8:60177906-60177925	MsG0880045503.01.T01:intron	30.0%
	GCAAGTGTATCAAAATTAAG+CGG	+	Chr8:60175333-60175352	None:intergenic	30.0%
	TAGCTGAGGATACAATAAAA+TGG	+	Chr8:60177155-60177174	None:intergenic	30.0%
	TCAGCTTGTCTTAATTTACT+TGG	-	Chr8:60177234-60177253	MsG0880045503.01.T01:intron	30.0%
	TGTTAATAATAGTCTCTGGT+TGG	-	Chr8:60177910-60177929	MsG0880045503.01.T01:intron	30.0%
	TTCCAAAATACTTTGGATGT+CGG	-	Chr8:60177990-60178009	MsG0880045503.01.T01:intron	30.0%
	TTCTCAAATATTAGCAGTGT+CGG	-	Chr8:60175542-60175561	MsG0880045503.01.T01:CDS	30.0%
	TTCTCACTGTATTACTATGA+TGG	-	Chr8:60176824-60176843	MsG0880045503.01.T01:intron	30.0%
	TTTAGGGGAGCTAAATTAAA+GGG	-	Chr8:60177108-60177127	MsG0880045503.01.T01:intron	30.0%
!	TGAAATGATTTAGGGACTTT+TGG	-	Chr8:60175616-60175635	MsG0880045503.01.T01:CDS	30.0%
!!	ACAATTTAATGGGTTTGTTC+AGG	-	Chr8:60176211-60176230	MsG0880045503.01.T01:intron	30.0%
!!	AGAAGTGTTGAATTAGAAGA+TGG	-	Chr8:60177697-60177716	MsG0880045503.01.T01:intron	30.0%
!!!	CTTGGACAAGAGTTTTTTAA+GGG	-	Chr8:60178041-60178060	MsG0880045503.01.T01:CDS	30.0%
!!!	GACTTTTGGTTTTATCTTGT+AGG	-	Chr8:60175630-60175649	MsG0880045503.01.T01:CDS	30.0%
!!!	TATAGAATGGTTTTAGCTGT+TGG	-	Chr8:60176036-60176055	MsG0880045503.01.T01:intron	30.0%
!!!	TCATTTTTTTATTCACCACC+TGG	+	Chr8:60177554-60177573	None:intergenic	30.0%
!!!	TCTTGGACAAGAGTTTTTTA+AGG	-	Chr8:60178040-60178059	MsG0880045503.01.T01:CDS	30.0%
	AAAACAAGTGGCTAAACCTT+TGG	-	Chr8:60177722-60177741	MsG0880045503.01.T01:intron	35.0%
	AAATTCTTGCCAGCAATTGT+AGG	+	Chr8:60176270-60176289	None:intergenic	35.0%
	AACATGCTTAAGAACCTAAC+TGG	-	Chr8:60176698-60176717	MsG0880045503.01.T01:intron	35.0%
	AATGTATTCATGCACACTGT+TGG	-	Chr8:60176503-60176522	MsG0880045503.01.T01:intron	35.0%
	ACAACTTCATAGATCTTCAC+CGG	+	Chr8:60174853-60174872	None:intergenic	35.0%
	ACATAAATTAGTGGTCCCTT+TGG	-	Chr8:60176905-60176924	MsG0880045503.01.T01:intron	35.0%
	ACTGGGGTTGTTCAATTAAA+AGG	-	Chr8:60176161-60176180	MsG0880045503.01.T01:intron	35.0%
	AGGCTGTGTAATTAAAGAAG+AGG	-	Chr8:60176628-60176647	MsG0880045503.01.T01:intron	35.0%
	AGGTCAATAGGATCCATTTA+TGG	-	Chr8:60176076-60176095	MsG0880045503.01.T01:intron	35.0%
	ATTGCAAAATCAGTTGTCAC+CGG	-	Chr8:60174907-60174926	MsG0880045503.01.T01:three_prime_UTR	35.0%
	CAATAGGATCCATTTATGGA+GGG	-	Chr8:60176080-60176099	MsG0880045503.01.T01:intron	35.0%
	CTGCGTTATGAGTTATTAGT+AGG	-	Chr8:60175423-60175442	MsG0880045503.01.T01:CDS	35.0%
	CTGGGGTTGTTCAATTAAAA+GGG	-	Chr8:60176162-60176181	MsG0880045503.01.T01:intron	35.0%
	CTGTGTAATTAAAGAAGAGG+TGG	-	Chr8:60176631-60176650	MsG0880045503.01.T01:intron	35.0%
	GAAATACAACAGATCTAGCA+GGG	-	Chr8:60177392-60177411	MsG0880045503.01.T01:intron	35.0%
	GAAGAAACCTAGAAGGTAAA+CGG	+	Chr8:60174679-60174698	None:intergenic	35.0%
	GGAATTTGAACAAACCCTAT+CGG	+	Chr8:60175090-60175109	None:intergenic	35.0%
	GTGTAAAGCTATTCATGTTC+TGG	-	Chr8:60175205-60175224	MsG0880045503.01.T01:three_prime_UTR	35.0%
	GTTTACCTTCTAGGTTTCTT+CGG	-	Chr8:60174678-60174697	MsG0880045503.01.T01:three_prime_UTR	35.0%
	GTTTAGGGGAGCTAAATTAA+AGG	-	Chr8:60177107-60177126	MsG0880045503.01.T01:intron	35.0%
	GTTTATTGTTGTGTTGTGTG+AGG	+	Chr8:60177446-60177465	None:intergenic	35.0%
	TAAAGCTATTCATGTTCTGG+TGG	-	Chr8:60175208-60175227	MsG0880045503.01.T01:three_prime_UTR	35.0%
	TAGAAGATGGAGAAAACAAG+TGG	-	Chr8:60177710-60177729	MsG0880045503.01.T01:intron	35.0%
	TCAATAGGATCCATTTATGG+AGG	-	Chr8:60176079-60176098	MsG0880045503.01.T01:intron	35.0%
	TGAAATACAACAGATCTAGC+AGG	-	Chr8:60177391-60177410	MsG0880045503.01.T01:intron	35.0%
	TGATCCCTTGCAACATTATT+GGG	+	Chr8:60175751-60175770	None:intergenic	35.0%
	TGGCACTTTCCAAAATACTT+TGG	-	Chr8:60177983-60178002	MsG0880045503.01.T01:intron	35.0%
	TTGATCCCTTGCAACATTAT+TGG	+	Chr8:60175752-60175771	None:intergenic	35.0%
	TTGTTGCAAGTACCAATAGA+GGG	-	Chr8:60175183-60175202	MsG0880045503.01.T01:three_prime_UTR	35.0%
	TTTAAGGGATATTCTTGTGC+CGG	-	Chr8:60178056-60178075	MsG0880045503.01.T01:CDS	35.0%
	TTTCAATTCCAACCACAACA+AGG	+	Chr8:60176474-60176493	None:intergenic	35.0%
	TTTGCAATTTCTTCGTCTTC+CGG	+	Chr8:60174895-60174914	None:intergenic	35.0%
!	AACCAACTGTATGCTCTTTT+TGG	+	Chr8:60177509-60177528	None:intergenic	35.0%
!	AAGTCACTCTGAGAAAATCA+AGG	+	Chr8:60176364-60176383	None:intergenic	35.0%
!	AATAGCTTTACACCCTCTAT+TGG	+	Chr8:60175198-60175217	None:intergenic	35.0%
!	AGCATACAGTTGGTTTCAAA+AGG	-	Chr8:60177514-60177533	MsG0880045503.01.T01:CDS	35.0%
!	GATAGGGTTTGTTCAAATTC+CGG	-	Chr8:60175089-60175108	MsG0880045503.01.T01:three_prime_UTR	35.0%
!	GTTGGTTTCAAAAGGACTAT+TGG	-	Chr8:60177522-60177541	MsG0880045503.01.T01:CDS	35.0%
!	TCTTCTGTTTTGCTTAGAGA+TGG	-	Chr8:60175235-60175254	MsG0880045503.01.T01:three_prime_UTR	35.0%
!	TCTTGTAGGAAATTAGGTTG+AGG	-	Chr8:60175644-60175663	MsG0880045503.01.T01:intron	35.0%
!	TTTGGAGCTTCACAAAATCA+TGG	+	Chr8:60175839-60175858	None:intergenic	35.0%
!!	AATAATTCAGGTTGGTACTG+TGG	-	Chr8:60177185-60177204	MsG0880045503.01.T01:intron	35.0%
!!	ATAATTCAGGTTGGTACTGT+GGG	-	Chr8:60177186-60177205	MsG0880045503.01.T01:intron	35.0%
!!	CTTGTAGGAAATTAGGTTGA+GGG	-	Chr8:60175645-60175664	MsG0880045503.01.T01:intron	35.0%
!!	TACCGACATCCAAAGTATTT+TGG	+	Chr8:60177995-60178014	None:intergenic	35.0%
!!	TGAGGGATTTGTTGATTGAA+TGG	-	Chr8:60175662-60175681	MsG0880045503.01.T01:intron	35.0%
!!	TGCTGAGTTGAAGTTTTTCT+TGG	-	Chr8:60175302-60175321	MsG0880045503.01.T01:three_prime_UTR	35.0%
!!	TTTCTGTTTCTGACAGAAAG+CGG	-	Chr8:60176561-60176580	MsG0880045503.01.T01:CDS	35.0%
!!!	AGGAACGGTTTTAGGATTAA+AGG	+	Chr8:60176454-60176473	None:intergenic	35.0%
	AAAGAAGACTCTCGAAAACC+AGG	-	Chr8:60174756-60174775	MsG0880045503.01.T01:three_prime_UTR	40.0%
	AATGGCCCAATAATGTTGCA+AGG	-	Chr8:60175743-60175762	MsG0880045503.01.T01:intron	40.0%
	ACAGAAAAGGTTGCATCTAG+TGG	-	Chr8:60177622-60177641	MsG0880045503.01.T01:intron	40.0%
	AGAAAATCATGCTCGGTAAG+TGG	+	Chr8:60177047-60177066	None:intergenic	40.0%
	AGGTTCGATTACGATTCGAT+TGG	-	Chr8:60175047-60175066	MsG0880045503.01.T01:three_prime_UTR	40.0%
	AGTGTTGTGAAATGCCGATA+GGG	-	Chr8:60175073-60175092	MsG0880045503.01.T01:three_prime_UTR	40.0%
	ATCGTAATCGAACCTTCCAA+CGG	+	Chr8:60175042-60175061	None:intergenic	40.0%
	ATGGATGTCATTGGAATCTC+AGG	-	Chr8:60176391-60176410	MsG0880045503.01.T01:intron	40.0%
	ATGGCCCAATAATGTTGCAA+GGG	-	Chr8:60175744-60175763	MsG0880045503.01.T01:intron	40.0%
	ATTCAACACCTTAGTCCATG+TGG	-	Chr8:60177646-60177665	MsG0880045503.01.T01:intron	40.0%
	ATTCCAACCACAACAAGGAA+CGG	+	Chr8:60176469-60176488	None:intergenic	40.0%
	ATTCCAATGACATCCATGTC+AGG	+	Chr8:60176388-60176407	None:intergenic	40.0%
	ATTGAATGGATCCCTTGCAA+GGG	-	Chr8:60175676-60175695	MsG0880045503.01.T01:intron	40.0%
	CTTCTAGGTTTCTTCGGTAT+CGG	-	Chr8:60174684-60174703	MsG0880045503.01.T01:three_prime_UTR	40.0%
	CTTGTTGCAAGTACCAATAG+AGG	-	Chr8:60175182-60175201	MsG0880045503.01.T01:three_prime_UTR	40.0%
	GATCTATGAAGTTGTTGCAC+CGG	-	Chr8:60174858-60174877	MsG0880045503.01.T01:three_prime_UTR	40.0%
	GGTTCGATTACGATTCGATT+GGG	-	Chr8:60175048-60175067	MsG0880045503.01.T01:three_prime_UTR	40.0%
	GGTTTCAAAAGGACTATTGG+TGG	-	Chr8:60177525-60177544	MsG0880045503.01.T01:CDS	40.0%
	TTCAAAAGGACTATTGGTGG+TGG	-	Chr8:60177528-60177547	MsG0880045503.01.T01:CDS	40.0%
	TTCAACACCTTAGTCCATGT+GGG	-	Chr8:60177647-60177666	MsG0880045503.01.T01:intron	40.0%
	TTCCAATGACATCCATGTCA+GGG	+	Chr8:60176387-60176406	None:intergenic	40.0%
	TTGCAACATTATTGGGCCAT+TGG	+	Chr8:60175744-60175763	None:intergenic	40.0%
	TTTAGCGAGAATCTCTTCGT+CGG	+	Chr8:60174739-60174758	None:intergenic	40.0%
	TTTAGCTGTTGGAGTTGTTG+CGG	-	Chr8:60176047-60176066	MsG0880045503.01.T01:intron	40.0%
!	ATCTCTAGAAGGTTTACCGT+TGG	-	Chr8:60175023-60175042	MsG0880045503.01.T01:three_prime_UTR	40.0%
!	CACTGTTGTTAGGTTTTCGT+CGG	-	Chr8:60175269-60175288	MsG0880045503.01.T01:three_prime_UTR	40.0%
!	CTGGATTCTAAGGAATACGA+CGG	+	Chr8:60174940-60174959	None:intergenic	40.0%
!	GACCAAAAAGAGCATACAGT+TGG	-	Chr8:60177504-60177523	MsG0880045503.01.T01:CDS	40.0%
!	TTTGTCCTTATGAGAGGCAT+TGG	+	Chr8:60177481-60177500	None:intergenic	40.0%
!!	ATGACTAGAGCACTGTTGTT+AGG	-	Chr8:60175259-60175278	MsG0880045503.01.T01:three_prime_UTR	40.0%
!!	ATTTTAGTGTGCTCTGTGTG+TGG	-	Chr8:60177832-60177851	MsG0880045503.01.T01:CDS	40.0%
!!	CAATCCTTGTCTTTTGCTGT+TGG	-	Chr8:60177963-60177982	MsG0880045503.01.T01:intron	40.0%
!!	GAAAGTGTTGAAGACTAGTG+TGG	-	Chr8:60176658-60176677	MsG0880045503.01.T01:intron	40.0%
!!!	GTCGTTTTTGTCCTTATGAG+AGG	+	Chr8:60177487-60177506	None:intergenic	40.0%
	AAGGTCCTTGCCATCATTCA+CGG	+	Chr8:60176853-60176872	None:intergenic	45.0%
	AATAGCCTAGCATTTGCACC+TGG	+	Chr8:60177285-60177304	None:intergenic	45.0%
	AATCTCCAGGTGCAAATGCT+AGG	-	Chr8:60177277-60177296	MsG0880045503.01.T01:intron	45.0%
	ACAATTGGAGTTACCGTTGC+CGG	+	Chr8:60174826-60174845	None:intergenic	45.0%
	AGAACTCTCACATTCTGAGC+AGG	+	Chr8:60176808-60176827	None:intergenic	45.0%
	AGCATTCAGCCTACAATTGC+TGG	-	Chr8:60176258-60176277	MsG0880045503.01.T01:intron	45.0%
	AGGTGCAAATGCTAGGCTAT+TGG	-	Chr8:60177284-60177303	MsG0880045503.01.T01:intron	45.0%
	AGTGCCAACAGCAAAAGACA+AGG	+	Chr8:60177970-60177989	None:intergenic	45.0%
	ATCTTCACCGGAGCTACAAT+TGG	+	Chr8:60174841-60174860	None:intergenic	45.0%
	ATGTACCTCAATGGAAGGCA+TGG	+	Chr8:60176889-60176908	None:intergenic	45.0%
	ATTCGATTCCGTTGCAGTAC+TGG	-	Chr8:60176294-60176313	MsG0880045503.01.T01:intron	45.0%
	CAGTGTTGTGAAATGCCGAT+AGG	-	Chr8:60175072-60175091	MsG0880045503.01.T01:three_prime_UTR	45.0%
	CCTAAAACCGTTCCTTGTTG+TGG	-	Chr8:60176459-60176478	MsG0880045503.01.T01:intron	45.0%
	CGATACCGAAGAAACCTAGA+AGG	+	Chr8:60174686-60174705	None:intergenic	45.0%
	CTAGAAGGTTTACCGTTGGA+AGG	-	Chr8:60175027-60175046	MsG0880045503.01.T01:three_prime_UTR	45.0%
	CTCACTCCATCTAACAACAG+TGG	+	Chr8:60175129-60175148	None:intergenic	45.0%
	GAAACCTAGAAGGTAAACGG+CGG	+	Chr8:60174676-60174695	None:intergenic	45.0%
	GATTGAATGGATCCCTTGCA+AGG	-	Chr8:60175675-60175694	MsG0880045503.01.T01:intron	45.0%
	GCAGCTGTGAACTGGATTAA+GGG	-	Chr8:60176583-60176602	MsG0880045503.01.T01:CDS	45.0%
	GCATGGTGACAGAAATGTGA+AGG	+	Chr8:60176872-60176891	None:intergenic	45.0%
	GGAATACGACGGAATTGAAC+CGG	+	Chr8:60174929-60174948	None:intergenic	45.0%
	GGAGGCTATAAGAGAAACGA+TGG	+	Chr8:60174655-60174674	None:intergenic	45.0%
	GGCTATAAGAGAAACGATGG+CGG	+	Chr8:60174652-60174671	None:intergenic	45.0%
	GGTAACTCCAATTGTAGCTC+CGG	-	Chr8:60174831-60174850	MsG0880045503.01.T01:three_prime_UTR	45.0%
	GTTTGGATTGATCGCGAGTT+TGG	-	Chr8:60175797-60175816	MsG0880045503.01.T01:intron	45.0%
	TACAGAGGGACAATATGGAG+AGG	-	Chr8:60175932-60175951	MsG0880045503.01.T01:intron	45.0%
	TCAACACCTTAGTCCATGTG+GGG	-	Chr8:60177648-60177667	MsG0880045503.01.T01:intron	45.0%
	TGAAGCACAAATACGGACAC+TGG	+	Chr8:60175590-60175609	None:intergenic	45.0%
	TGGTAAATCTGTGGTGTGTG+AGG	-	Chr8:60176608-60176627	MsG0880045503.01.T01:intron	45.0%
	TTCCCTGACATGGATGTCAT+TGG	-	Chr8:60176382-60176401	MsG0880045503.01.T01:intron	45.0%
!	AAACCGTTCCTTGTTGTGGT+TGG	-	Chr8:60176463-60176482	MsG0880045503.01.T01:intron	45.0%
!	AGTGTTGAAGACTAGTGTGG+AGG	-	Chr8:60176661-60176680	MsG0880045503.01.T01:intron	45.0%
!	ATGATGGAACCCGTGAATGA+TGG	-	Chr8:60176840-60176859	MsG0880045503.01.T01:intron	45.0%
!	CCACAACAAGGAACGGTTTT+AGG	+	Chr8:60176462-60176481	None:intergenic	45.0%
!	GGGATGAACATGGTGTCAAA+AGG	-	Chr8:60176327-60176346	MsG0880045503.01.T01:intron	45.0%
!	GGGTTTGTTCAAATTCCGGT+TGG	-	Chr8:60175093-60175112	MsG0880045503.01.T01:three_prime_UTR	45.0%
!	GTCTGCTAGTCTGGATTCTA+AGG	+	Chr8:60174950-60174969	None:intergenic	45.0%
!	TATAAGAGAAACGATGGCGG+CGG	+	Chr8:60174649-60174668	None:intergenic	45.0%
!	TTAAGGGCGTGGTAAATCTG+TGG	-	Chr8:60176599-60176618	MsG0880045503.01.T01:intron	45.0%
!	TTGAGCTCAACAAGAGCATG+TGG	-	Chr8:60177587-60177606	MsG0880045503.01.T01:intron	45.0%
!!	ACTGTTGGTTTGATTGTGGC+AGG	-	Chr8:60176518-60176537	MsG0880045503.01.T01:CDS	45.0%
!!	GCACACTGTTGGTTTGATTG+TGG	-	Chr8:60176514-60176533	MsG0880045503.01.T01:CDS	45.0%
	AAAGACAATGCTCCCCCACA+TGG	+	Chr8:60177664-60177683	None:intergenic	50.0%
	AACATGGAGAGGGGATACAG+AGG	-	Chr8:60175917-60175936	MsG0880045503.01.T01:intron	50.0%
	ACATGGAGAGGGGATACAGA+GGG	-	Chr8:60175918-60175937	MsG0880045503.01.T01:intron	50.0%
	ACCGACGTAAAACCCACAAC+CGG	-	Chr8:60174804-60174823	MsG0880045503.01.T01:three_prime_UTR	50.0%
	ACTAGCAGACTGCCGTAAAG+CGG	-	Chr8:60174960-60174979	MsG0880045503.01.T01:three_prime_UTR	50.0%
	ACTGCCAGTCAAACATGGAG+AGG	-	Chr8:60175906-60175925	MsG0880045503.01.T01:intron	50.0%
	AGACATGAGTGATAGCTCGC+CGG	+	Chr8:60177337-60177356	None:intergenic	50.0%
	AGGTCCTTGCCATCATTCAC+GGG	+	Chr8:60176852-60176871	None:intergenic	50.0%
	ATCCCCTCTCCATGTTTGAC+TGG	+	Chr8:60175913-60175932	None:intergenic	50.0%
	ATGGAGGGACGAGGTCATAT+AGG	-	Chr8:60176095-60176114	MsG0880045503.01.T01:intron	50.0%
	ATTCTGAGCAGGATCCTGAC+CGG	+	Chr8:60176797-60176816	None:intergenic	50.0%
	CAACACCTTAGTCCATGTGG+GGG	-	Chr8:60177649-60177668	MsG0880045503.01.T01:intron	50.0%
	CTATTGGCCACCATTGTAGC+TGG	-	Chr8:60177300-60177319	MsG0880045503.01.T01:intron	50.0%
	CTCATGTCTGCAATTGCAGC+TGG	-	Chr8:60177348-60177367	MsG0880045503.01.T01:intron	50.0%
	CTGCCAGTCAAACATGGAGA+GGG	-	Chr8:60175907-60175926	MsG0880045503.01.T01:intron	50.0%
	GATCCATTTATGGAGGGACG+AGG	-	Chr8:60176086-60176105	MsG0880045503.01.T01:intron	50.0%
	GCAGGTCCACTGTTGTTAGA+TGG	-	Chr8:60175120-60175139	MsG0880045503.01.T01:three_prime_UTR	50.0%
	GGCAGCTGTGAACTGGATTA+AGG	-	Chr8:60176582-60176601	MsG0880045503.01.T01:CDS	50.0%
	GGGGATACAGAGGGACAATA+TGG	-	Chr8:60175927-60175946	MsG0880045503.01.T01:intron	50.0%
	GTAAAACCCACAACCGGCAA+CGG	-	Chr8:60174810-60174829	MsG0880045503.01.T01:three_prime_UTR	50.0%
	GTCTCACTGCCAGTCAAACA+TGG	-	Chr8:60175901-60175920	MsG0880045503.01.T01:intron	50.0%
	TACCGAACCAGCTACAATGG+TGG	+	Chr8:60177310-60177329	None:intergenic	50.0%
	TCAGAGTGACTTCCCTGACA+TGG	-	Chr8:60176372-60176391	MsG0880045503.01.T01:intron	50.0%
	TCAGCGGTGCAAACGATAAC+TGG	-	Chr8:60174997-60175016	MsG0880045503.01.T01:three_prime_UTR	50.0%
	TCGCGAGTTTGGCTAAGTCA+TGG	-	Chr8:60175808-60175827	MsG0880045503.01.T01:intron	50.0%
	TCTGCTGTCTACATAGCCAC+CGG	-	Chr8:60176775-60176794	MsG0880045503.01.T01:intron	50.0%
	TCTTCCGGTGTGAGATTCAC+CGG	+	Chr8:60174880-60174899	None:intergenic	50.0%
	TGACACCATGTTCATCCCCA+TGG	+	Chr8:60176325-60176344	None:intergenic	50.0%
	TGACCTCGTCCCTCCATAAA+TGG	+	Chr8:60176092-60176111	None:intergenic	50.0%
	TGATGCCATGGGGATGAACA+TGG	-	Chr8:60176317-60176336	MsG0880045503.01.T01:intron	50.0%
	TGCAACAAGACACCCTTCAG+TGG	+	Chr8:60175171-60175190	None:intergenic	50.0%
	TGCCAGTCAAACATGGAGAG+GGG	-	Chr8:60175908-60175927	MsG0880045503.01.T01:intron	50.0%
	TGGCATCACCAGTACTGCAA+CGG	+	Chr8:60176305-60176324	None:intergenic	50.0%
	TGTCACCATGCCTTCCATTG+AGG	-	Chr8:60176881-60176900	MsG0880045503.01.T01:intron	50.0%
	TGTGAACTGGATTAAGGGCG+TGG	-	Chr8:60176588-60176607	MsG0880045503.01.T01:CDS	50.0%
	TTGCGGATGGAGAGGTCAAT+AGG	-	Chr8:60176064-60176083	MsG0880045503.01.T01:intron	50.0%
	TTTACGGCAGTCTGCTAGTC+TGG	+	Chr8:60174959-60174978	None:intergenic	50.0%
!	GCAAGCTCACGAGACGATTT+TGG	+	Chr8:60177424-60177443	None:intergenic	50.0%
!!	ATTCAGGTTGGTACTGTGGG+AGG	-	Chr8:60177189-60177208	MsG0880045503.01.T01:intron	50.0%
!!	CAAATTCCGGTTGGTGTTGC+AGG	-	Chr8:60175102-60175121	MsG0880045503.01.T01:three_prime_UTR	50.0%
!!	GAGTACCGAACCAGCTACAA+TGG	+	Chr8:60177313-60177332	None:intergenic	50.0%
!!	TGCAGTACTGGTGATGCCAT+GGG	-	Chr8:60176306-60176325	MsG0880045503.01.T01:intron	50.0%
!!	TTCAGGTTGGTACTGTGGGA+GGG	-	Chr8:60177190-60177209	MsG0880045503.01.T01:intron	50.0%
!!	TTGCAGTACTGGTGATGCCA+TGG	-	Chr8:60176305-60176324	MsG0880045503.01.T01:intron	50.0%
	ACACCCTTCAGTGGTAGCCA+TGG	+	Chr8:60175162-60175181	None:intergenic	55.0%
	ACCTAGAAGGTAAACGGCGG+AGG	+	Chr8:60174673-60174692	None:intergenic	55.0%
	AGAAAGCGGCAGCTGTGAAC+TGG	-	Chr8:60176575-60176594	MsG0880045503.01.T01:CDS	55.0%
	AGTGGACCTGCAACACCAAC+CGG	+	Chr8:60175111-60175130	None:intergenic	55.0%
	CACCCTTCAGTGGTAGCCAT+GGG	+	Chr8:60175161-60175180	None:intergenic	55.0%
	CAGCAAGGGCTGATCCAGTT+AGG	+	Chr8:60176715-60176734	None:intergenic	55.0%
	CGGATCACGTACTTCCTTCC+CGG	-	Chr8:60174704-60174723	MsG0880045503.01.T01:three_prime_UTR	55.0%
	CTAACTGGATCAGCCCTTGC+TGG	-	Chr8:60176713-60176732	MsG0880045503.01.T01:intron	55.0%
	GAGTGAGTACACTGTGCCCA+TGG	-	Chr8:60175142-60175161	MsG0880045503.01.T01:three_prime_UTR	55.0%
	GCTGTTGGAGTTGTTGCGGA+TGG	-	Chr8:60176051-60176070	MsG0880045503.01.T01:intron	55.0%
	GGAACCCGTGAATGATGGCA+AGG	-	Chr8:60176845-60176864	MsG0880045503.01.T01:intron	55.0%
	GGCCACCATTGTAGCTGGTT+CGG	-	Chr8:60177305-60177324	MsG0880045503.01.T01:intron	55.0%
	GGGGACCAATGCCTCTCATA+AGG	-	Chr8:60177473-60177492	MsG0880045503.01.T01:intron	55.0%
	TCTTCGTCGGAGAGATCGTC+CGG	+	Chr8:60174726-60174745	None:intergenic	55.0%
	TGCACCTGGAGATTCTGTGC+TGG	+	Chr8:60177271-60177290	None:intergenic	55.0%
	TGCCCATGGCTACCACTGAA+GGG	-	Chr8:60175156-60175175	MsG0880045503.01.T01:three_prime_UTR	55.0%
	TGGAGTTGTTGCGGATGGAG+AGG	-	Chr8:60176056-60176075	MsG0880045503.01.T01:intron	55.0%
	TGTCTACATAGCCACCGGTC+AGG	-	Chr8:60176780-60176799	MsG0880045503.01.T01:intron	55.0%
!	ATGCTCCCCCACATGGACTA+AGG	+	Chr8:60177657-60177676	None:intergenic	55.0%
!	GAAGACTAGTGTGGAGGCAC+TGG	-	Chr8:60176667-60176686	MsG0880045503.01.T01:intron	55.0%
!	GACGTATTGCCGCCGCTTTA+CGG	+	Chr8:60174975-60174994	None:intergenic	55.0%
!	GAGTTACCGTTGCCGGTTGT+GGG	+	Chr8:60174819-60174838	None:intergenic	55.0%
!	GCCGGTTGTGGGTTTTACGT+CGG	+	Chr8:60174808-60174827	None:intergenic	55.0%
!	TCGACAAGTGCTGCTGGACA+CGG	+	Chr8:60174783-60174802	None:intergenic	55.0%
!	TGCACCGGTGAATCTCACAC+CGG	-	Chr8:60174873-60174892	MsG0880045503.01.T01:three_prime_UTR	55.0%
!!	AAGGACTATTGGTGGTGGCC+AGG	-	Chr8:60177533-60177552	MsG0880045503.01.T01:CDS	55.0%
!!	GCAGTACTGGTGATGCCATG+GGG	-	Chr8:60176307-60176326	MsG0880045503.01.T01:intron	55.0%
!!	TCAGGTTGGTACTGTGGGAG+GGG	-	Chr8:60177191-60177210	MsG0880045503.01.T01:intron	55.0%
	ACGGTCCTGCAACCCTTGCA+AGG	+	Chr8:60175691-60175710	None:intergenic	60.0%
	AGCAGACTGCCGTAAAGCGG+CGG	-	Chr8:60174963-60174982	MsG0880045503.01.T01:three_prime_UTR	60.0%
	AGCCCTTGCTGGTGCTCTTG+GGG	-	Chr8:60176724-60176743	MsG0880045503.01.T01:intron	60.0%
	CAGCCCTTGCTGGTGCTCTT+GGG	-	Chr8:60176723-60176742	MsG0880045503.01.T01:intron	60.0%
	CGGTCCTGCAACCCTTGCAA+GGG	+	Chr8:60175690-60175709	None:intergenic	60.0%
	CTTCGTCGGAGAGATCGTCC+GGG	+	Chr8:60174725-60174744	None:intergenic	60.0%
	GCCTCCGCCGTTTACCTTCT+AGG	-	Chr8:60174669-60174688	MsG0880045503.01.T01:three_prime_UTR	60.0%
	GGATCCCTTGCAAGGGTTGC+AGG	-	Chr8:60175683-60175702	MsG0880045503.01.T01:intron	60.0%
	GGCGGCAATACGTCGTTCAG+CGG	-	Chr8:60174981-60175000	MsG0880045503.01.T01:three_prime_UTR	60.0%
	GTGCCCATGGCTACCACTGA+AGG	-	Chr8:60175155-60175174	MsG0880045503.01.T01:three_prime_UTR	60.0%
	TCAGCCCTTGCTGGTGCTCT+TGG	-	Chr8:60176722-60176741	MsG0880045503.01.T01:intron	60.0%
	TGCAAGGGTTGCAGGACCGT+TGG	-	Chr8:60175691-60175710	MsG0880045503.01.T01:intron	60.0%
!	GGAGTTACCGTTGCCGGTTG+TGG	+	Chr8:60174820-60174839	None:intergenic	60.0%
!!	CAGGTTGGTACTGTGGGAGG+GGG	-	Chr8:60177192-60177211	MsG0880045503.01.T01:intron	60.0%
!!	GACTATTGGTGGTGGCCAGG+TGG	-	Chr8:60177536-60177555	MsG0880045503.01.T01:CDS	60.0%
!!	GTAGCTGGTTCGGTACTCGC+CGG	-	Chr8:60177315-60177334	MsG0880045503.01.T01:intron	60.0%
!!	TCGGTGTCGACAAGTGCTGC+TGG	+	Chr8:60174789-60174808	None:intergenic	60.0%
	CCCTTGCTGGTGCTCTTGGG+GGG	-	Chr8:60176726-60176745	MsG0880045503.01.T01:intron	65.0%
	CTGAGCAGGATCCTGACCGG+TGG	+	Chr8:60176794-60176813	None:intergenic	65.0%
	GCCCTTGCTGGTGCTCTTGG+GGG	-	Chr8:60176725-60176744	MsG0880045503.01.T01:intron	65.0%
	GGCGCCAGCACAGAATCTCC+AGG	-	Chr8:60177264-60177283	MsG0880045503.01.T01:intron	65.0%
	GTCGGAGAGATCGTCCGGGA+AGG	+	Chr8:60174721-60174740	None:intergenic	65.0%
!	AGTGCTGCTGGACACGGTCC+TGG	+	Chr8:60174777-60174796	None:intergenic	65.0%
!!	ACCCCCCAAGAGCACCAGCA+AGG	+	Chr8:60176730-60176749	None:intergenic	65.0%
!!	CCCCCCAAGAGCACCAGCAA+GGG	+	Chr8:60176729-60176748	None:intergenic	65.0%

Chromosome	Type	Strat	End	Strand	Name
Chr8	gene	60174639	60178205	60174639	ID=MsG0880045503.01;Name=MsG0880045503.01
Chr8	mRNA	60174639	60178205	60174639	ID=MsG0880045503.01.T01;Parent=MsG0880045503.01;Name=MsG0880045503.01.T01;_AED=0.49;_eAED=0.49;_QI=0\|0\|0.2\|1\|0.5\|0.4\|5\|768\|267
Chr8	exon	60174639	60175649	60174639	ID=MsG0880045503.01.T01:exon:12431;Parent=MsG0880045503.01.T01
Chr8	exon	60176506	60176611	60176506	ID=MsG0880045503.01.T01:exon:12430;Parent=MsG0880045503.01.T01
Chr8	exon	60177477	60177563	60177477	ID=MsG0880045503.01.T01:exon:12429;Parent=MsG0880045503.01.T01
Chr8	exon	60177750	60177924	60177750	ID=MsG0880045503.01.T01:exon:12428;Parent=MsG0880045503.01.T01
Chr8	exon	60178013	60178205	60178013	ID=MsG0880045503.01.T01:exon:12427;Parent=MsG0880045503.01.T01
Chr8	CDS	60178013	60178205	60178013	ID=MsG0880045503.01.T01:cds;Parent=MsG0880045503.01.T01
Chr8	CDS	60177750	60177924	60177750	ID=MsG0880045503.01.T01:cds;Parent=MsG0880045503.01.T01
Chr8	CDS	60177477	60177563	60177477	ID=MsG0880045503.01.T01:cds;Parent=MsG0880045503.01.T01
Chr8	CDS	60176506	60176611	60176506	ID=MsG0880045503.01.T01:cds;Parent=MsG0880045503.01.T01
Chr8	CDS	60175407	60175649	60175407	ID=MsG0880045503.01.T01:cds;Parent=MsG0880045503.01.T01
Chr8	three_prime_UTR	60174639	60175406	60174639	ID=MsG0880045503.01.T01:three_prime_utr;Parent=MsG0880045503.01.T01

Gene Sequence

>MsG0880045503.01.T01

TCTGAAACCGCCGCCATCGTTTCTCTTATAGCCTCCGCCGTTTACCTTCTAGGTTTCTTCGGTATCGGATCACGTACTTCCTTCCCGGACGATCTCTCCGACGAAGAGATTCTCGCTAAAGAAGACTCTCGAAAACCAGGACCGTGTCCAGCAGCACTTGTCGACACCGACGTAAAACCCACAACCGGCAACGTTGTCACCGGTTCAATTCCGTCGTATTCCTTAGAATCCAGACTAGCAGACTGCCGTAAAGCGGCGGCAATACGTCGTTCAGCGGTGCAAACGATAACTGGAAAATCTCTAGAAGGTTTACCGTTGGAAGGTTCGATTACGATTCGATTGGGACAGTGTTGTGAAATGCCGATAGGGTTTTCGTCGGCAAAAAGAGCTGCTGAGTTGAAGTTTTTCTTGGAAGATCCGCTTAATTTTGATACACTTGCTGTCACTTTCAACAAGTCAAGCAGATTTGCTAGATTGCAGAGCATTCAGCCTACAATTGCTGGCAAGAATTTGTACATTCGATTCCGTTGCAGTACTGGTGATGCCATGGGGATGAACATGGTTGGTACTGTGGGAGGGGGAACACAACTTGCATCTCAGTCAGCTTGTCTTAATTTACTTGGTGTTAAGGGCGCCAGCACAGAATCTCCAGGTGCAAATGCTAGGCTATTGGCCACCATTGTAGCTGGTTCGGTACTCGCCGGCGAGCTATCACTCATGTCTGCAATTGCAGCTGGACAACTAGTTAAGAGTCACATGAAATACAACAGATCTAGCAGGGATATGTCCAAAATCGTCTCGTGA

Protein sequence

>MsG0880045503.01.T01

SETAAIVSLIASAVYLLGFFGIGSRTSFPDDLSDEEILAKEDSRKPGPCPAALVDTDVKPTTGNVVTGSIPSYSLESRLADCRKAAAIRRSAVQTITGKSLEGLPLEGSITIRLGQCCEMPIGFSSAKRAAELKFFLEDPLNFDTLAVTFNKSSRFARLQSIQPTIAGKNLYIRFRCSTGDAMGMNMVGTVGGGTQLASQSACLNLLGVKGASTESPGANARLLATIVAGSVLAGELSLMSAIAAGQLVKSHMKYNRSSRDMSKIVS*