Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780040743.01.T03 | GAU11654.1 | 97.5 | 40 | 1 | 0 | 1 | 40 | 147 | 186 | 1.56E-18 | 83.6 |
| MsG0780040743.01.T02 | GAU11654.1 | 94.872 | 39 | 2 | 0 | 1 | 39 | 1 | 39 | 2.03E-16 | 78.2 |
| MsG0780040743.01.T01 | XP_013449898.1 | 85.065 | 154 | 12 | 1 | 2 | 144 | 36 | 189 | 2.70E-88 | 265 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780040743.01.T01 | A0A072U3J7 | 85.065 | 154 | 12 | 1 | 2 | 144 | 36 | 189 | 1.29e-88 | 265 |
| MsG0780040743.01.T02 | A0A2Z6LGF4 | 94.872 | 39 | 2 | 0 | 1 | 39 | 1 | 39 | 9.67e-17 | 78.2 |
| MsG0780040743.01.T03 | A0A2Z6LGF4 | 97.500 | 40 | 1 | 0 | 1 | 40 | 147 | 186 | 7.43e-19 | 83.6 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0480023596.01 | MsG0780040743.01 | 0.800334 | 1.513304e-48 | 7.027927e-46 |
| MsG0580025184.01 | MsG0780040743.01 | 0.800457 | 1.428634e-48 | 6.655121e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780040743.01.T03 | MTR_7g096950 | 97.500 | 40 | 1 | 0 | 1 | 40 | 150 | 189 | 4.32e-22 | 82.8 |
| MsG0780040743.01.T02 | MTR_7g096950 | 94.872 | 39 | 2 | 0 | 1 | 39 | 1 | 39 | 3.37e-20 | 77.8 |
| MsG0780040743.01.T01 | MTR_7g096950 | 85.065 | 154 | 12 | 1 | 2 | 144 | 36 | 189 | 3.28e-92 | 265 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 32 sgRNAs with CRISPR-Local
Find 36 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CGCTTAGCAGCAAGGAATTT+AGG | 0.230904 | 7:+81852390 | None:intergenic |
| TTCGCAGTTATTTCGATTTG+TGG | 0.337470 | 7:+81852222 | None:intergenic |
| AGGTGCCTTGGTAGTGATAC+TGG | 0.352156 | 7:+81852410 | None:intergenic |
| TCATCAATTCCTTGCATTTG+AGG | 0.408360 | 7:+81852087 | None:intergenic |
| TCGATTTGTGGTCTGTGAAT+TGG | 0.422489 | 7:+81852234 | None:intergenic |
| AAGAGCTCATCAATATAAAC+AGG | 0.429364 | 7:+81852303 | None:intergenic |
| AGCAAGGAATTTAGGTGCCT+TGG | 0.442470 | 7:+81852398 | None:intergenic |
| CTCTTCCATACCTTTCTAGC+AGG | 0.462977 | 7:+81852533 | None:intergenic |
| TTCTAGCAGGTGGAAGCCTT+AGG | 0.468112 | 7:+81852546 | None:intergenic |
| ATCGAAATAACTGCGAAGAT+TGG | 0.468530 | 7:-81852216 | MsG0780040743.01.T01:intron |
| AAGAGCTTCACTTCTAAACT+TGG | 0.469460 | 7:-81852515 | MsG0780040743.01.T02:CDS |
| CCATGTCTTGCATAAGCCTA+AGG | 0.482076 | 7:-81852562 | MsG0780040743.01.T02:CDS |
| AAGGCTTCCACCTGCTAGAA+AGG | 0.483331 | 7:-81852543 | MsG0780040743.01.T02:CDS |
| GGTCTGTGAATTGGTGTTTG+TGG | 0.486464 | 7:+81852243 | None:intergenic |
| AGAGCCTCCTTGTGACTTAG+TGG | 0.489786 | 7:-81852277 | MsG0780040743.01.T01:CDS |
| TTCCATACCTTTCTAGCAGG+TGG | 0.498563 | 7:+81852536 | None:intergenic |
| CAGAAAACAGATGGCAGCGC+AGG | 0.511725 | 7:-81852037 | MsG0780040743.01.T01:CDS |
| TCATCAATATAAACAGGTGC+TGG | 0.515522 | 7:+81852309 | None:intergenic |
| CTAGTGCATCAGATGATATG+TGG | 0.535580 | 7:-81852131 | MsG0780040743.01.T01:CDS |
| CCTTAGGCTTATGCAAGACA+TGG | 0.565799 | 7:+81852562 | None:intergenic |
| TTCCACCTGCTAGAAAGGTA+TGG | 0.580026 | 7:-81852538 | MsG0780040743.01.T02:CDS |
| GCCGAAAACGCTTAGCAGCA+AGG | 0.583139 | 7:+81852382 | None:intergenic |
| GAGCCTCCTTGTGACTTAGT+GGG | 0.583807 | 7:-81852276 | MsG0780040743.01.T01:CDS |
| TACCAGGTTCAGAAAACAGA+TGG | 0.601074 | 7:-81852046 | MsG0780040743.01.T01:CDS |
| TAGTGCATCAGATGATATGT+GGG | 0.604307 | 7:-81852130 | MsG0780040743.01.T01:CDS |
| TTAGCTTCACCTCAAATGCA+AGG | 0.619041 | 7:-81852096 | MsG0780040743.01.T01:CDS |
| AGGAATTGATGAACGAGCAG+AGG | 0.631742 | 7:-81852076 | MsG0780040743.01.T01:CDS |
| GAGCAGAGGAGTTCATTACC+AGG | 0.638794 | 7:-81852062 | MsG0780040743.01.T01:CDS |
| AGATGATATGTGGGAGTCTG+TGG | 0.644635 | 7:-81852121 | MsG0780040743.01.T01:CDS |
| ATCTACCAGTATCACTACCA+AGG | 0.668685 | 7:-81852415 | MsG0780040743.01.T01:CDS |
| AAATAACCCACTAAGTCACA+AGG | 0.670657 | 7:+81852270 | None:intergenic |
| TAACCCACTAAGTCACAAGG+AGG | 0.740102 | 7:+81852273 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | GATGCTTTTTTGTTTTTTCA+TGG | + | Chr7:81852117-81852136 | None:intergenic | 25.0% |
| AAGAGCTCATCAATATAAAC+AGG | + | Chr7:81852274-81852293 | None:intergenic | 30.0% | |
| ! | GGTAGATGATTTTATAGCTT+TGG | + | Chr7:81852146-81852165 | None:intergenic | 30.0% |
| AAATAACCCACTAAGTCACA+AGG | + | Chr7:81852307-81852326 | None:intergenic | 35.0% | |
| AAGAGCTTCACTTCTAAACT+TGG | - | Chr7:81852059-81852078 | MsG0780040743.01.T01:CDS | 35.0% | |
| ATCGAAATAACTGCGAAGAT+TGG | - | Chr7:81852358-81852377 | MsG0780040743.01.T01:CDS | 35.0% | |
| TAGTGCATCAGATGATATGT+GGG | - | Chr7:81852444-81852463 | MsG0780040743.01.T01:CDS | 35.0% | |
| TCAAAGAGAGAAGAAAGAAG+TGG | - | Chr7:81852387-81852406 | MsG0780040743.01.T01:CDS | 35.0% | |
| TCATCAATATAAACAGGTGC+TGG | + | Chr7:81852268-81852287 | None:intergenic | 35.0% | |
| TCATCAATTCCTTGCATTTG+AGG | + | Chr7:81852490-81852509 | None:intergenic | 35.0% | |
| ! | TTCGCAGTTATTTCGATTTG+TGG | + | Chr7:81852355-81852374 | None:intergenic | 35.0% |
| ATCTACCAGTATCACTACCA+AGG | - | Chr7:81852159-81852178 | MsG0780040743.01.T01:CDS | 40.0% | |
| CTAGTGCATCAGATGATATG+TGG | - | Chr7:81852443-81852462 | MsG0780040743.01.T01:CDS | 40.0% | |
| TACCAGGTTCAGAAAACAGA+TGG | - | Chr7:81852528-81852547 | MsG0780040743.01.T02:CDS | 40.0% | |
| TTAGCTTCACCTCAAATGCA+AGG | - | Chr7:81852478-81852497 | MsG0780040743.01.T02:CDS | 40.0% | |
| ! | TCGATTTGTGGTCTGTGAAT+TGG | + | Chr7:81852343-81852362 | None:intergenic | 40.0% |
| AGATGATATGTGGGAGTCTG+TGG | - | Chr7:81852453-81852472 | MsG0780040743.01.T01:CDS | 45.0% | |
| AGCAAGGAATTTAGGTGCCT+TGG | + | Chr7:81852179-81852198 | None:intergenic | 45.0% | |
| AGGAATTGATGAACGAGCAG+AGG | - | Chr7:81852498-81852517 | MsG0780040743.01.T02:CDS | 45.0% | |
| CGCTTAGCAGCAAGGAATTT+AGG | + | Chr7:81852187-81852206 | None:intergenic | 45.0% | |
| GAGAAGAAAGAAGTGGCAGA+AGG | - | Chr7:81852394-81852413 | MsG0780040743.01.T01:CDS | 45.0% | |
| TAACCCACTAAGTCACAAGG+AGG | + | Chr7:81852304-81852323 | None:intergenic | 45.0% | |
| TTCCACCTGCTAGAAAGGTA+TGG | - | Chr7:81852036-81852055 | MsG0780040743.01.T01:CDS | 45.0% | |
| ! | CTCTTCCATACCTTTCTAGC+AGG | + | Chr7:81852044-81852063 | None:intergenic | 45.0% |
| ! | GGTCTGTGAATTGGTGTTTG+TGG | + | Chr7:81852334-81852353 | None:intergenic | 45.0% |
| ! | TCCTTGCTGCTAAGCGTTTT+CGG | - | Chr7:81852191-81852210 | MsG0780040743.01.T01:intron | 45.0% |
| ! | TGCCATCTGTTTTCTGAACC+TGG | + | Chr7:81852533-81852552 | None:intergenic | 45.0% |
| ! | TTCCATACCTTTCTAGCAGG+TGG | + | Chr7:81852041-81852060 | None:intergenic | 45.0% |
| AAGGCTTCCACCTGCTAGAA+AGG | - | Chr7:81852031-81852050 | MsG0780040743.01.T01:CDS | 50.0% | |
| AGAGCCTCCTTGTGACTTAG+TGG | - | Chr7:81852297-81852316 | MsG0780040743.01.T01:CDS | 50.0% | |
| GAGCAGAGGAGTTCATTACC+AGG | - | Chr7:81852512-81852531 | MsG0780040743.01.T02:CDS | 50.0% | |
| GAGCCTCCTTGTGACTTAGT+GGG | - | Chr7:81852298-81852317 | MsG0780040743.01.T01:CDS | 50.0% | |
| TTCTAGCAGGTGGAAGCCTT+AGG | + | Chr7:81852031-81852050 | None:intergenic | 50.0% | |
| !! | AGGTGCCTTGGTAGTGATAC+TGG | + | Chr7:81852167-81852186 | None:intergenic | 50.0% |
| CAGAAAACAGATGGCAGCGC+AGG | - | Chr7:81852537-81852556 | MsG0780040743.01.T02:CDS | 55.0% | |
| GCCGAAAACGCTTAGCAGCA+AGG | + | Chr7:81852195-81852214 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr7 | gene | 81852014 | 81852582 | 81852014 | ID=MsG0780040743.01;Name=MsG0780040743.01 |
| Chr7 | mRNA | 81852014 | 81852481 | 81852014 | ID=MsG0780040743.01.T01;Parent=MsG0780040743.01;Name=MsG0780040743.01.T01;_AED=0.30;_eAED=0.31;_QI=0|0|0|1|0|0|2|0|144 |
| Chr7 | exon | 81852014 | 81852183 | 81852014 | ID=MsG0780040743.01.T01:exon:19075;Parent=MsG0780040743.01.T01 |
| Chr7 | exon | 81852217 | 81852481 | 81852217 | ID=MsG0780040743.01.T01:exon:19074;Parent=MsG0780040743.01.T01 |
| Chr7 | CDS | 81852217 | 81852481 | 81852217 | ID=MsG0780040743.01.T01:cds;Parent=MsG0780040743.01.T01 |
| Chr7 | CDS | 81852014 | 81852183 | 81852014 | ID=MsG0780040743.01.T01:cds;Parent=MsG0780040743.01.T01 |
| Chr7 | mRNA | 81852457 | 81852582 | 81852457 | ID=MsG0780040743.01.T02;Parent=MsG0780040743.01;Name=MsG0780040743.01.T02;_AED=0.44;_eAED=0.44;_QI=0|-1|0|1|-1|0|1|0|41 |
| Chr7 | exon | 81852457 | 81852582 | 81852457 | ID=MsG0780040743.01.T02:exon:19076;Parent=MsG0780040743.01.T02 |
| Chr7 | CDS | 81852457 | 81852582 | 81852457 | ID=MsG0780040743.01.T02:cds;Parent=MsG0780040743.01.T02 |
| Chr7 | mRNA | 81852014 | 81852136 | 81852014 | ID=MsG0780040743.01.T03;Parent=MsG0780040743.01;Name=MsG0780040743.01.T03;_AED=0.44;_eAED=0.44;_QI=0|-1|0|1|-1|0|1|0|40 |
| Chr7 | exon | 81852014 | 81852136 | 81852014 | ID=MsG0780040743.01.T03:exon:19077;Parent=MsG0780040743.01.T03 |
| Chr7 | CDS | 81852014 | 81852136 | 81852014 | ID=MsG0780040743.01.T03:cds;Parent=MsG0780040743.01.T03 |
| Gene Sequence |
| Protein sequence |
