Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780040824.01.T01 | XP_003625443.1 | 99.074 | 108 | 0 | 1 | 1 | 108 | 1 | 107 | 1.02E-69 | 214 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780040824.01.T01 | G7KYK0 | 99.074 | 108 | 0 | 1 | 1 | 108 | 1 | 107 | 4.88e-70 | 214 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180000337.01 | MsG0780040824.01 | 0.815018 | 1.145956e-51 | 7.810610e-49 |
| MsG0780040824.01 | MsG0880043744.01 | 0.817642 | 2.966851e-52 | 2.172661e-49 |
| MsG0280009394.01 | MsG0780040824.01 | 0.834485 | 2.917007e-56 | 3.466715e-53 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780040824.01.T01 | MTR_7g099200 | 99.074 | 108 | 0 | 1 | 1 | 108 | 1 | 107 | 1.24e-73 | 214 |
| MsG0780040824.01.T01 | MTR_1g069370 | 92.784 | 97 | 7 | 0 | 3 | 99 | 7 | 103 | 1.09e-64 | 191 |
| MsG0780040824.01.T01 | MTR_1g076840 | 56.842 | 95 | 41 | 0 | 1 | 95 | 38 | 132 | 8.35e-38 | 124 |
| MsG0780040824.01.T01 | MTR_6g060170 | 55.789 | 95 | 42 | 0 | 1 | 95 | 4 | 98 | 1.61e-37 | 122 |
| MsG0780040824.01.T01 | MTR_6g059920 | 47.674 | 86 | 45 | 0 | 7 | 92 | 40 | 125 | 9.37e-25 | 90.9 |
| MsG0780040824.01.T01 | MTR_5g005820 | 44.706 | 85 | 47 | 0 | 7 | 91 | 9 | 93 | 8.07e-23 | 85.1 |
| MsG0780040824.01.T01 | MTR_6g060000 | 57.778 | 45 | 19 | 0 | 34 | 78 | 17 | 61 | 3.35e-13 | 59.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780040824.01.T01 | AT3G04300 | 67.368 | 95 | 31 | 0 | 5 | 99 | 1 | 95 | 6.43e-46 | 143 |
| MsG0780040824.01.T01 | AT4G28703 | 63.366 | 101 | 28 | 1 | 6 | 97 | 7 | 107 | 2.27e-41 | 132 |
| MsG0780040824.01.T01 | AT4G10300 | 61.538 | 91 | 35 | 0 | 5 | 95 | 44 | 134 | 9.30e-40 | 129 |
| MsG0780040824.01.T01 | AT4G10280 | 36.559 | 93 | 56 | 1 | 7 | 96 | 48 | 140 | 7.31e-18 | 73.9 |
| MsG0780040824.01.T01 | AT4G10290 | 34.343 | 99 | 62 | 1 | 7 | 102 | 20 | 118 | 2.64e-13 | 61.6 |
Find 21 sgRNAs with CRISPR-Local
Find 43 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGTCTGAAGCCATGAGGTTT+TGG | 0.241413 | 7:-82997201 | None:intergenic |
| TTACATGCAATGTGGGATTT+TGG | 0.280274 | 7:-82997122 | None:intergenic |
| AATTTCAATTGGTACTTTCC+TGG | 0.284978 | 7:-82997422 | None:intergenic |
| CAAGTACAACTGAGTCCTTT+TGG | 0.330475 | 7:-82997548 | None:intergenic |
| CAAAAGGACTCAGTTGTACT+TGG | 0.347089 | 7:+82997549 | MsG0780040824.01.T01:CDS |
| CTGAGAAGGGTTTCTTTCAA+TGG | 0.378765 | 7:-82997233 | None:intergenic |
| CTTCTGCATCAAATTTCAAT+TGG | 0.414470 | 7:-82997433 | None:intergenic |
| AAAAGATGGGGTTGTTCTCC+AGG | 0.417937 | 7:+82997404 | MsG0780040824.01.T01:intron |
| AAACATGTTATTTATTGAAA+GGG | 0.428832 | 7:+82997456 | MsG0780040824.01.T01:CDS |
| GAAACATGTTATTTATTGAA+AGG | 0.444821 | 7:+82997455 | MsG0780040824.01.T01:CDS |
| AAAGTGAAAGCATACACAAA+AGG | 0.467313 | 7:+82997479 | MsG0780040824.01.T01:CDS |
| TTCTTTCAATGGTGATTCGA+AGG | 0.500926 | 7:-82997222 | None:intergenic |
| CAGCTAAACGTGACTGAGAA+GGG | 0.513726 | 7:-82997246 | None:intergenic |
| GACCTTGTCACCATTCCAAA+AGG | 0.519526 | 7:+82997533 | MsG0780040824.01.T01:CDS |
| AAGTGAAAGCATACACAAAA+GGG | 0.539574 | 7:+82997480 | MsG0780040824.01.T01:CDS |
| GTTCTCTTCCCAAAACCTCA+TGG | 0.549774 | 7:+82997192 | MsG0780040824.01.T01:exon |
| ATTAATAGTAATTAACAAGA+TGG | 0.549911 | 7:-82997641 | None:intergenic |
| CTGAATTGAACATCAAGTGC+TGG | 0.553924 | 7:+82997266 | MsG0780040824.01.T01:CDS |
| TCAGCTAAACGTGACTGAGA+AGG | 0.572317 | 7:-82997247 | None:intergenic |
| AAAAGGACTCAGTTGTACTT+GGG | 0.580307 | 7:+82997550 | MsG0780040824.01.T01:CDS |
| TTCGAAGGTCTGAAGCCATG+AGG | 0.682433 | 7:-82997207 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATTAATAGTAATTAACAAGA+TGG | - | Chr7:82997644-82997663 | None:intergenic | 15.0% |
| !!! | AAACATGTTATTTATTGAAA+GGG | + | Chr7:82997456-82997475 | MsG0780040824.01.T01:CDS | 15.0% |
| !! | ATTAAGAAAGAATACATACT+TGG | - | Chr7:82997293-82997312 | None:intergenic | 20.0% |
| !! | TTAAGAAAGAATACATACTT+GGG | - | Chr7:82997292-82997311 | None:intergenic | 20.0% |
| !!! | GAAACATGTTATTTATTGAA+AGG | + | Chr7:82997455-82997474 | MsG0780040824.01.T01:CDS | 20.0% |
| !!! | TTTTTGCATTAATCTAACAA+AGG | + | Chr7:82997342-82997361 | MsG0780040824.01.T01:intron | 20.0% |
| !!! | TTTTTTGTTACATGCAATGT+GGG | - | Chr7:82997132-82997151 | None:intergenic | 25.0% |
| !!! | TTTTTTTGTTACATGCAATG+TGG | - | Chr7:82997133-82997152 | None:intergenic | 25.0% |
| AATTTCAATTGGTACTTTCC+TGG | - | Chr7:82997425-82997444 | None:intergenic | 30.0% | |
| CTTCTGCATCAAATTTCAAT+TGG | - | Chr7:82997436-82997455 | None:intergenic | 30.0% | |
| GGGGTGTAAAAATAAAAGAT+GGG | + | Chr7:82997391-82997410 | MsG0780040824.01.T01:intron | 30.0% | |
| GGGTGTAAAAATAAAAGATG+GGG | + | Chr7:82997392-82997411 | MsG0780040824.01.T01:intron | 30.0% | |
| ! | AAAGTGAAAGCATACACAAA+AGG | + | Chr7:82997479-82997498 | MsG0780040824.01.T01:CDS | 30.0% |
| ! | AAGTGAAAGCATACACAAAA+GGG | + | Chr7:82997480-82997499 | MsG0780040824.01.T01:CDS | 30.0% |
| ! | TCATCAGATTTTGTAGAGTT+TGG | + | Chr7:82997503-82997522 | MsG0780040824.01.T01:CDS | 30.0% |
| !! | TTTTGGTGGATAGAAAAAGA+AGG | - | Chr7:82997108-82997127 | None:intergenic | 30.0% |
| AAAAGGACTCAGTTGTACTT+GGG | + | Chr7:82997550-82997569 | MsG0780040824.01.T01:CDS | 35.0% | |
| CAGCCACATTACAAAACAAA+AGG | - | Chr7:82997369-82997388 | None:intergenic | 35.0% | |
| GTGGATAGAAAAAGAAGGAA+TGG | - | Chr7:82997103-82997122 | None:intergenic | 35.0% | |
| TTCTTTCAATGGTGATTCGA+AGG | - | Chr7:82997225-82997244 | None:intergenic | 35.0% | |
| ! | GGGGGTGTAAAAATAAAAGA+TGG | + | Chr7:82997390-82997409 | MsG0780040824.01.T01:intron | 35.0% |
| ! | TTACATGCAATGTGGGATTT+TGG | - | Chr7:82997125-82997144 | None:intergenic | 35.0% |
| !! | TTGTTTTGTAATGTGGCTGT+GGG | + | Chr7:82997370-82997389 | MsG0780040824.01.T01:intron | 35.0% |
| !!! | GGTCCTTTTGTTTTGTAATG+TGG | + | Chr7:82997363-82997382 | MsG0780040824.01.T01:intron | 35.0% |
| !!! | TTTGTTTTGTAATGTGGCTG+TGG | + | Chr7:82997369-82997388 | MsG0780040824.01.T01:intron | 35.0% |
| CAAAAGGACTCAGTTGTACT+TGG | + | Chr7:82997549-82997568 | MsG0780040824.01.T01:CDS | 40.0% | |
| CTGAATTGAACATCAAGTGC+TGG | + | Chr7:82997266-82997285 | MsG0780040824.01.T01:CDS | 40.0% | |
| CTGAGAAGGGTTTCTTTCAA+TGG | - | Chr7:82997236-82997255 | None:intergenic | 40.0% | |
| ! | CAAGTACAACTGAGTCCTTT+TGG | - | Chr7:82997551-82997570 | None:intergenic | 40.0% |
| ! | TGTTTTGTAATGTGGCTGTG+GGG | + | Chr7:82997371-82997390 | MsG0780040824.01.T01:intron | 40.0% |
| !! | ACAACTGAGTCCTTTTGGAA+TGG | - | Chr7:82997546-82997565 | None:intergenic | 40.0% |
| !! | GATTTTGTAGAGTTTGGAGC+TGG | + | Chr7:82997509-82997528 | MsG0780040824.01.T01:CDS | 40.0% |
| AAAAGATGGGGTTGTTCTCC+AGG | + | Chr7:82997404-82997423 | MsG0780040824.01.T01:intron | 45.0% | |
| CAGCTAAACGTGACTGAGAA+GGG | - | Chr7:82997249-82997268 | None:intergenic | 45.0% | |
| GACCTTGTCACCATTCCAAA+AGG | + | Chr7:82997533-82997552 | MsG0780040824.01.T01:CDS | 45.0% | |
| GTTCTCTTCCCAAAACCTCA+TGG | + | Chr7:82997192-82997211 | MsG0780040824.01.T01:exon | 45.0% | |
| TCAGCTAAACGTGACTGAGA+AGG | - | Chr7:82997250-82997269 | None:intergenic | 45.0% | |
| ! | CATGCAATGTGGGATTTTGG+TGG | - | Chr7:82997122-82997141 | None:intergenic | 45.0% |
| ! | GTTTTGTAATGTGGCTGTGG+GGG | + | Chr7:82997372-82997391 | MsG0780040824.01.T01:intron | 45.0% |
| !! | GTCTGAAGCCATGAGGTTTT+GGG | - | Chr7:82997203-82997222 | None:intergenic | 45.0% |
| !!! | GTCCTTTTGGAATGGTGACA+AGG | - | Chr7:82997538-82997557 | None:intergenic | 45.0% |
| ! | TTCGAAGGTCTGAAGCCATG+AGG | - | Chr7:82997210-82997229 | None:intergenic | 50.0% |
| !! | GGTCTGAAGCCATGAGGTTT+TGG | - | Chr7:82997204-82997223 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr7 | gene | 82997074 | 82997712 | 82997074 | ID=MsG0780040824.01;Name=MsG0780040824.01 |
| Chr7 | mRNA | 82997074 | 82997712 | 82997074 | ID=MsG0780040824.01.T01;Parent=MsG0780040824.01;Name=MsG0780040824.01.T01;_AED=0.47;_eAED=0.47;_QI=137|1|1|1|0|0|2|60|108 |
| Chr7 | exon | 82997074 | 82997293 | 82997074 | ID=MsG0780040824.01.T01:exon:19560;Parent=MsG0780040824.01.T01 |
| Chr7 | exon | 82997409 | 82997712 | 82997409 | ID=MsG0780040824.01.T01:exon:19561;Parent=MsG0780040824.01.T01 |
| Chr7 | five_prime_UTR | 82997074 | 82997210 | 82997074 | ID=MsG0780040824.01.T01:five_prime_utr;Parent=MsG0780040824.01.T01 |
| Chr7 | CDS | 82997211 | 82997293 | 82997211 | ID=MsG0780040824.01.T01:cds;Parent=MsG0780040824.01.T01 |
| Chr7 | CDS | 82997409 | 82997652 | 82997409 | ID=MsG0780040824.01.T01:cds;Parent=MsG0780040824.01.T01 |
| Chr7 | three_prime_UTR | 82997653 | 82997712 | 82997653 | ID=MsG0780040824.01.T01:three_prime_utr;Parent=MsG0780040824.01.T01 |
| Chr7 | mRNA | 82997460 | 82997591 | 82997460 | ID=MsG0780040824.01.T02;Parent=MsG0780040824.01;Name=MsG0780040824.01.T02;_AED=0.49;_eAED=0.49;_QI=0|-1|0|1|-1|0|1|0|43 |
| Chr7 | exon | 82997460 | 82997591 | 82997460 | ID=MsG0780040824.01.T02:exon:19562;Parent=MsG0780040824.01.T02 |
| Chr7 | CDS | 82997460 | 82997591 | 82997460 | ID=MsG0780040824.01.T02:cds;Parent=MsG0780040824.01.T02 |
| Gene Sequence |
| Protein sequence |
