AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180000337.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180000337.01.T01 MTR_1g017830 76.190 126 2 2 1 103 40 160 2.99e-56 171
MsG0180000337.01.T01 MTR_3g103290 49.541 109 25 3 15 100 53 154 1.48e-20 80.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 14 sgRNAs with CRISPR-Local

Find 17 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCTGCTTCTGCTAGAACTTT+AGG 0.258479 1:-4607261 MsG0180000337.01.T01:intron
TCTAGTGTTGAAGGAAAAGT+TGG 0.393697 1:-4607121 MsG0180000337.01.T01:CDS
ACAGCATAACTCTTTGATCT+AGG 0.434486 1:+4607079 None:intergenic
AAGATCATCAGCATCATTAG+AGG 0.441233 1:-4607313 MsG0180000337.01.T01:CDS
TGTGGTCAAAATATGAGTTA+TGG 0.456641 1:-4607417 None:intergenic
CCTTCAACACTAGAATCAAA+AGG 0.478362 1:+4607130 None:intergenic
AATTTACTAGCAGCATCCAT+TGG 0.526181 1:+4607387 None:intergenic
TTACCTAAGTCTAGTAGTGT+TGG 0.528478 1:-4607178 MsG0180000337.01.T01:CDS
ATTCCAACACTACTAGACTT+AGG 0.543949 1:+4607175 None:intergenic
TAAGTCTAGTAGTGTTGGAA+TGG 0.550381 1:-4607173 MsG0180000337.01.T01:CDS
AGATCAAAGAGTTATGCTGT+TGG 0.581741 1:-4607076 MsG0180000337.01.T01:CDS
GAGTTATGGAAGTTATCCAA+TGG 0.620886 1:-4607403 MsG0180000337.01.T01:CDS
AGATCATCAGCATCATTAGA+GGG 0.626119 1:-4607312 MsG0180000337.01.T01:CDS
TGGAATGGCTAAGATTGATG+AGG 0.704946 1:-4607158 MsG0180000337.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! ACAACAACAGAATTGAATAA+AGG - Chr1:4607251-4607270 MsG0180000337.01.T01:intron 25.0%
! CAACAACAGAATTGAATAAA+GGG - Chr1:4607252-4607271 MsG0180000337.01.T01:intron 25.0%
! TTTAGGTAATAGAATTGATG+TGG - Chr1:4607209-4607228 MsG0180000337.01.T01:intron 25.0%
AAGATCATCAGCATCATTAG+AGG - Chr1:4607140-4607159 MsG0180000337.01.T01:CDS 35.0%
AATTTACTAGCAGCATCCAT+TGG + Chr1:4607069-4607088 None:intergenic 35.0%
ACAGCATAACTCTTTGATCT+AGG + Chr1:4607377-4607396 None:intergenic 35.0%
AGATCAAAGAGTTATGCTGT+TGG - Chr1:4607377-4607396 MsG0180000337.01.T01:CDS 35.0%
AGATCATCAGCATCATTAGA+GGG - Chr1:4607141-4607160 MsG0180000337.01.T01:CDS 35.0%
ATTCCAACACTACTAGACTT+AGG + Chr1:4607281-4607300 None:intergenic 35.0%
CCTTCAACACTAGAATCAAA+AGG + Chr1:4607326-4607345 None:intergenic 35.0%
GAGTTATGGAAGTTATCCAA+TGG - Chr1:4607050-4607069 MsG0180000337.01.T01:CDS 35.0%
TTACCTAAGTCTAGTAGTGT+TGG - Chr1:4607275-4607294 MsG0180000337.01.T01:CDS 35.0%
! TAAGTCTAGTAGTGTTGGAA+TGG - Chr1:4607280-4607299 MsG0180000337.01.T01:CDS 35.0%
! TCTAGTGTTGAAGGAAAAGT+TGG - Chr1:4607332-4607351 MsG0180000337.01.T01:CDS 35.0%
!! CCTTTTGATTCTAGTGTTGA+AGG - Chr1:4607323-4607342 MsG0180000337.01.T01:CDS 35.0%
TGGAATGGCTAAGATTGATG+AGG - Chr1:4607295-4607314 MsG0180000337.01.T01:CDS 40.0%
GCTGCTTCTGCTAGAACTTT+AGG - Chr1:4607192-4607211 MsG0180000337.01.T01:intron 45.0%
Chromosome Type Strat End Strand Name
Chr1 gene 4607048 4607427 4607048 ID=MsG0180000337.01;Name=MsG0180000337.01
Chr1 mRNA 4607048 4607427 4607048 ID=MsG0180000337.01.T01;Parent=MsG0180000337.01;Name=MsG0180000337.01.T01;_AED=0.44;_eAED=0.46;_QI=0|0|0|1|1|1|2|0|106
Chr1 exon 4607262 4607427 4607262 ID=MsG0180000337.01.T01:exon:5450;Parent=MsG0180000337.01.T01
Chr1 exon 4607048 4607202 4607048 ID=MsG0180000337.01.T01:exon:5451;Parent=MsG0180000337.01.T01
Chr1 CDS 4607262 4607427 4607262 ID=MsG0180000337.01.T01:cds;Parent=MsG0180000337.01.T01
Chr1 CDS 4607048 4607202 4607048 ID=MsG0180000337.01.T01:cds;Parent=MsG0180000337.01.T01
Gene Sequence

>MsG0180000337.01.T01

ATGAGTTATGGAAGTTATCCAATGGATGCTGCTAGTAAATTTTCTTTATCAAGAAGTCAAAGTGCAGCAACATCAAGAAGATCATCATCAAGAAGATCATCAGCATCATTAGAGGGTAATGAAGATTTTGCTGAGCTTGTTAGAGCTGCTTCTGCTAGAACTTTAGGGTTACCTAAGTCTAGTAGTGTTGGAATGGCTAAGATTGATGAGGATATGCCTTTTGATTCTAGTGTTGAAGGAAAAGTTGGTTTTGTTGCTGATTCTTATCCTAGATCAAAGAGTTATGCTGTTGGACATAGAAGAAATGTTGTTGCTTTCTGA

Protein sequence

>MsG0180000337.01.T01

MSYGSYPMDAASKFSLSRSQSAATSRRSSSRRSSASLEGNEDFAELVRAASARTLGLPKSSSVGMAKIDEDMPFDSSVEGKVGFVADSYPRSKSYAVGHRRNVVAF*