AlfalfaGEDB | MsG0280009394.01

M. sativa cultivar ZhongmuNo.1 / MsG0280009394.01

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280009394.01.T01	XP_013464148.1	98.765	81	1	0	1	81	1	81	1.70E-48	158

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280009394.01.T01	I3SDA0	98.765	81	1	0	1	81	1	81	8.13e-49	158

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080048345.01	MsG0280009394.01	0.818556	1.843095e-52	1.384120e-49
MsG0080048348.01	MsG0280009394.01	0.801522	8.655198e-49	4.142166e-46
MsG0080048350.01	MsG0280009394.01	0.808815	2.572841e-50	1.485642e-47
MsG0080048352.01	MsG0280009394.01	0.814524	1.474330e-51	9.916127e-49
MsG0180000019.01	MsG0280009394.01	0.903827	2.319843e-79	3.490689e-75
MsG0180000337.01	MsG0280009394.01	0.886771	2.558554e-72	1.852192e-68
MsG0180001050.01	MsG0280009394.01	0.833959	3.952199e-56	4.623572e-53
MsG0180001053.01	MsG0280009394.01	0.834570	2.777422e-56	3.309376e-53
MsG0180003240.01	MsG0280009394.01	0.802096	6.597213e-49	3.203614e-46
MsG0180003687.01	MsG0280009394.01	0.846207	2.510359e-59	4.288237e-56
MsG0180003830.01	MsG0280009394.01	0.810580	1.074272e-50	6.500024e-48
MsG0180004110.01	MsG0280009394.01	0.871326	7.445058e-67	2.991292e-63
MsG0180004203.01	MsG0280009394.01	0.800364	1.492204e-48	6.935220e-46
MsG0180004562.01	MsG0280009394.01	0.892191	1.993524e-74	1.803300e-70
MsG0180004752.01	MsG0280009394.01	0.840368	9.068451e-58	1.288974e-54
MsG0180004867.01	MsG0280009394.01	0.817991	2.474612e-52	1.829653e-49
MsG0180004941.01	MsG0280009394.01	0.823968	1.040928e-53	9.098143e-51
MsG0180005328.01	MsG0280009394.01	0.823063	1.694777e-53	1.443969e-50
MsG0180005356.01	MsG0280009394.01	0.819806	9.569013e-53	7.440199e-50
MsG0180005357.01	MsG0280009394.01	0.837187	6.024985e-57	7.766190e-54
MsG0180005542.01	MsG0280009394.01	0.803752	3.000579e-49	1.520089e-46
MsG0180006012.01	MsG0280009394.01	0.856352	3.409002e-62	8.113788e-59
MsG0280006475.01	MsG0280009394.01	-0.811353	7.305150e-51	4.511521e-48
MsG0280006883.01	MsG0280009394.01	0.820533	6.524348e-53	5.175882e-50
MsG0280006974.01	MsG0280009394.01	0.802357	5.830059e-49	2.850110e-46
MsG0280007133.01	MsG0280009394.01	-0.819974	8.763075e-53	6.845367e-50
MsG0280007369.01	MsG0280009394.01	0.859535	3.868842e-63	1.025166e-59
MsG0280007391.01	MsG0280009394.01	0.800743	1.248909e-48	5.860391e-46
MsG0280007751.01	MsG0280009394.01	0.813674	2.270751e-51	1.492463e-48
MsG0280007759.01	MsG0280009394.01	0.819035	1.434918e-52	1.091999e-49
MsG0280007760.01	MsG0280009394.01	0.810648	1.038349e-50	6.294258e-48
MsG0280008405.01	MsG0280009394.01	0.833598	4.863253e-56	5.627481e-53
MsG0280009394.01	MsG0280009838.01	0.805421	1.346132e-49	7.118567e-47
MsG0280009394.01	MsG0280009971.01	0.804831	1.788744e-49	9.317222e-47
MsG0280009394.01	MsG0280011041.01	0.845708	3.430409e-59	5.767465e-56
MsG0280009394.01	MsG0280011042.01	0.826817	2.202706e-54	2.088257e-51
MsG0280009394.01	MsG0280011053.01	0.826210	3.073974e-54	2.864165e-51
MsG0280009394.01	MsG0280011165.01	0.822305	2.542747e-53	2.120285e-50
MsG0280009394.01	MsG0280011193.01	0.827289	1.698710e-54	1.632903e-51
MsG0280009394.01	MsG0280011203.01	0.822048	2.917393e-53	2.414695e-50
MsG0280009394.01	MsG0280011264.01	0.821727	3.461690e-53	2.839267e-50
MsG0280009394.01	MsG0280011473.01	0.822331	2.507757e-53	2.092699e-50
MsG0280009394.01	MsG0380011613.01	0.836808	7.529675e-57	9.595477e-54
MsG0280009394.01	MsG0380013300.01	0.854043	1.600933e-61	3.529735e-58
MsG0280009394.01	MsG0380014920.01	0.809784	1.594107e-50	9.446388e-48
MsG0280009394.01	MsG0380015010.01	0.859872	3.061152e-63	8.207058e-60
MsG0280009394.01	MsG0380016020.01	0.811362	7.270730e-51	4.491480e-48
MsG0280009394.01	MsG0380016800.01	0.810715	1.004022e-50	6.097341e-48
MsG0280009394.01	MsG0380016839.01	0.804248	2.366569e-49	1.214194e-46
MsG0280009394.01	MsG0380017020.01	0.826787	2.239283e-54	2.121068e-51
MsG0280009394.01	MsG0380017550.01	0.846824	1.704270e-59	2.970123e-56
MsG0280009394.01	MsG0480018333.01	0.815063	1.120026e-51	7.643030e-49
MsG0280009394.01	MsG0480018500.01	0.822550	2.231110e-53	1.873539e-50
MsG0280009394.01	MsG0480019038.01	0.802864	4.581715e-49	2.268879e-46
MsG0280009394.01	MsG0480019295.01	0.877491	6.020545e-69	3.033464e-65
MsG0280009394.01	MsG0480019880.01	0.801918	7.177094e-49	3.469429e-46
MsG0280009394.01	MsG0480020428.01	0.829157	6.023247e-55	6.114059e-52
MsG0280009394.01	MsG0480021135.01	0.830459	2.902048e-55	3.059080e-52
MsG0280009394.01	MsG0480022053.01	0.800348	1.503516e-48	6.984904e-46
MsG0280009394.01	MsG0480022125.01	0.875803	2.310687e-68	1.093919e-64
MsG0280009394.01	MsG0480022400.01	0.800818	1.205557e-48	5.667706e-46
MsG0280009394.01	MsG0480022655.01	0.821896	3.163148e-53	2.607042e-50
MsG0280009394.01	MsG0480022760.01	0.814412	1.560988e-51	1.046748e-48
MsG0280009394.01	MsG0480023107.01	0.827779	1.295376e-54	1.262957e-51
MsG0280009394.01	MsG0480023318.01	0.810008	1.427328e-50	8.507638e-48
MsG0280009394.01	MsG0480023819.01	0.811931	5.468760e-51	3.429559e-48
MsG0280009394.01	MsG0480023947.01	0.825723	4.011837e-54	3.686604e-51
MsG0280009394.01	MsG0580024154.01	0.812959	3.260742e-51	2.101781e-48
MsG0280009394.01	MsG0580024871.01	0.813958	1.966329e-51	1.302305e-48
MsG0280009394.01	MsG0580024999.01	0.804605	1.994178e-49	1.032627e-46
MsG0280009394.01	MsG0580025440.01	0.818677	1.729911e-52	1.303458e-49
MsG0280009394.01	MsG0580025876.01	0.819444	1.157809e-52	8.911324e-50
MsG0280009394.01	MsG0580027073.01	0.844781	6.109926e-59	9.971709e-56
MsG0280009394.01	MsG0580027227.01	0.853170	2.852753e-61	6.107879e-58
MsG0280009394.01	MsG0580027733.01	0.846886	1.638444e-59	2.860868e-56
MsG0280009394.01	MsG0580028355.01	0.827186	1.797636e-54	1.722825e-51
MsG0280009394.01	MsG0580028560.01	0.802369	5.795591e-49	2.834153e-46
MsG0280009394.01	MsG0580028999.01	0.881075	3.242780e-70	1.873009e-66
MsG0280009394.01	MsG0580029555.01	0.821199	4.584104e-53	3.704977e-50
MsG0280009394.01	MsG0580029583.01	0.870974	9.722399e-67	3.856193e-63
MsG0280009394.01	MsG0580029626.01	0.805230	1.475491e-49	7.764512e-47
MsG0280009394.01	MsG0580029930.01	0.846718	1.821879e-59	3.164566e-56
MsG0280009394.01	MsG0680030315.01	0.828354	9.418816e-55	9.338940e-52
MsG0280009394.01	MsG0680031240.01	0.822416	2.396441e-53	2.004667e-50
MsG0280009394.01	MsG0680031429.01	0.814254	1.691637e-51	1.129447e-48
MsG0280009394.01	MsG0680035496.01	0.810865	9.318898e-51	5.681944e-48
MsG0280009394.01	MsG0780038123.01	0.866245	3.284076e-65	1.099361e-61
MsG0280009394.01	MsG0780039334.01	0.859409	4.221292e-63	1.113878e-59
MsG0280009394.01	MsG0780039472.01	0.835424	1.692171e-56	2.068408e-53
MsG0280009394.01	MsG0780039783.01	0.808515	2.981780e-50	1.708362e-47
MsG0280009394.01	MsG0780039877.01	0.868219	7.682073e-66	2.757194e-62
MsG0280009394.01	MsG0780039978.01	0.834353	3.147696e-56	3.726065e-53
MsG0280009394.01	MsG0780040144.01	0.848546	5.722903e-60	1.053906e-56
MsG0280009394.01	MsG0780040353.01	0.808947	2.411256e-50	1.397213e-47
MsG0280009394.01	MsG0780040480.01	0.800791	1.221120e-48	5.736752e-46
MsG0280009394.01	MsG0780040824.01	0.834485	2.917007e-56	3.466715e-53
MsG0280009394.01	MsG0780041198.01	0.800001	1.768420e-48	8.143387e-46
MsG0280009394.01	MsG0780041316.01	0.815384	9.502266e-52	6.541765e-49
MsG0280009394.01	MsG0780041380.01	0.839832	1.251861e-57	1.749870e-54
MsG0280009394.01	MsG0780041556.01	0.845212	4.674366e-59	7.733626e-56
MsG0280009394.01	MsG0780041648.01	0.800336	1.511539e-48	7.020161e-46
MsG0280009394.01	MsG0880041985.01	0.810894	9.183605e-51	5.603876e-48
MsG0280009394.01	MsG0880042495.01	0.804279	2.331227e-49	1.197076e-46
MsG0280009394.01	MsG0880043285.01	0.824969	6.049404e-54	5.441114e-51
MsG0280009394.01	MsG0880043685.01	0.837128	6.239607e-57	8.028041e-54
MsG0280009394.01	MsG0880043715.01	0.859257	4.686193e-63	1.229854e-59
MsG0280009394.01	MsG0880043744.01	0.896099	5.106479e-76	5.443395e-72
MsG0280009394.01	MsG0880043824.01	0.821856	3.231753e-53	2.660592e-50
MsG0280009394.01	MsG0880043828.01	0.872558	2.901142e-67	1.219532e-63
MsG0280009394.01	MsG0880044570.01	0.826227	3.045865e-54	2.839344e-51
MsG0280009394.01	MsG0880044669.01	0.823733	1.181780e-53	1.026234e-50
MsG0280009394.01	MsG0880045272.01	0.865257	6.733682e-65	2.176685e-61
MsG0280009394.01	MsG0880045275.01	0.834480	2.925359e-56	3.476100e-53
MsG0280009394.01	MsG0880045277.01	0.902163	1.283585e-78	1.794252e-74
MsG0280009394.01	MsG0880045441.01	0.839735	1.326104e-57	1.848036e-54
MsG0280009394.01	MsG0880046176.01	0.851098	1.107472e-60	2.216814e-57
MsG0280009394.01	MsG0880046996.01	0.863396	2.565634e-64	7.766206e-61
MsG0280009394.01	MsG0880047081.01	0.882605	9.055717e-71	5.550990e-67
MsG0280009394.01	MsG0880047486.01	0.878840	2.027509e-69	1.074440e-65
MsG0280009394.01	MsG0880047491.01	0.847701	9.788914e-60	1.754724e-56
MsG0280009394.01	MsG0880047531.01	0.831538	1.577095e-55	1.716661e-52

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280009394.01.T01	MTR_2g061780	98.765	81	1	0	1	81	1	81	2.06e-52	158

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280009394.01.T01	AT2G31945	59.375	64	24	1	1	62	1	64	1.70e-19	75.5
MsG0280009394.01.T01	AT1G05575	56.000	75	31	1	1	73	1	75	1.31e-17	70.5

Find 19 sgRNAs with CRISPR-Local

Find 20 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GTTTGATCTCTTGATGTTCT+TGG	0.211211	2:+51284920	None:intergenic
GAAGGAGAGGGTTCTCCTTC+AGG	0.307513	2:+51284834	None:intergenic
TTGATGAATTTGAAAGGATA+AGG	0.383924	2:+51284952	None:intergenic
CTCTGAGTTGCTTGTGATTG+AGG	0.403222	2:+51284801	None:intergenic
GATTGAGGACTCATTGTAGA+AGG	0.403857	2:+51284816	None:intergenic
CGTGAGTATTCGTCGGATGT+TGG	0.440469	2:-51284877	MsG0280009394.01.T01:CDS
GTTCGTCCTAGTCCAAGCTT+TGG	0.507181	2:-51284979	MsG0280009394.01.T01:CDS
CTTATGTGACATGTTATTGA+AGG	0.536891	2:-51285009	MsG0280009394.01.T01:CDS
GGATAAGGTACACCAAAGCT+TGG	0.548918	2:+51284967	None:intergenic
CGCGTTCCGTGAGTATTCGT+CGG	0.549054	2:-51284884	MsG0280009394.01.T01:CDS
ATATGTTTGATGAATTTGAA+AGG	0.553082	2:+51284946	None:intergenic
CGAATACTCACGGAACGCGC+CGG	0.585547	2:+51284888	None:intergenic
GAGATCAAACAGTTTCAAGC+CGG	0.604551	2:-51284907	MsG0280009394.01.T01:CDS
ATGTGACATGTTATTGAAGG+TGG	0.605175	2:-51285006	MsG0280009394.01.T01:CDS
AGGACTCATTGTAGAAGGAG+AGG	0.608887	2:+51284821	None:intergenic
GGTACACCAAAGCTTGGACT+AGG	0.611822	2:+51284973	None:intergenic
GGACTCATTGTAGAAGGAGA+GGG	0.615228	2:+51284822	None:intergenic
GGAGAGGGTTCTCCTTCAGG+AGG	0.644566	2:+51284837	None:intergenic
CAACATCCGACGAATACTCA+CGG	0.680704	2:+51284878	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	ATATGTTTGATGAATTTGAA+AGG	+	Chr2:51284874-51284893	None:intergenic	20.0%
!	TTGATGAATTTGAAAGGATA+AGG	+	Chr2:51284868-51284887	None:intergenic	25.0%
	CTTATGTGACATGTTATTGA+AGG	-	Chr2:51284808-51284827	MsG0280009394.01.T01:CDS	30.0%
	ATGTGACATGTTATTGAAGG+TGG	-	Chr2:51284811-51284830	MsG0280009394.01.T01:CDS	35.0%
	GTTTGATCTCTTGATGTTCT+TGG	+	Chr2:51284900-51284919	None:intergenic	35.0%
	GAGATCAAACAGTTTCAAGC+CGG	-	Chr2:51284910-51284929	MsG0280009394.01.T01:CDS	40.0%
	GATTGAGGACTCATTGTAGA+AGG	+	Chr2:51285004-51285023	None:intergenic	40.0%
	AGGACTCATTGTAGAAGGAG+AGG	+	Chr2:51284999-51285018	None:intergenic	45.0%
	CAACATCCGACGAATACTCA+CGG	+	Chr2:51284942-51284961	None:intergenic	45.0%
	CTCTGAGTTGCTTGTGATTG+AGG	+	Chr2:51285019-51285038	None:intergenic	45.0%
	GGACTCATTGTAGAAGGAGA+GGG	+	Chr2:51284998-51285017	None:intergenic	45.0%
	GGATAAGGTACACCAAAGCT+TGG	+	Chr2:51284853-51284872	None:intergenic	45.0%
!	CTCTCTGATTTTCCTCCTGA+AGG	-	Chr2:51284968-51284987	MsG0280009394.01.T01:CDS	45.0%
	CGTGAGTATTCGTCGGATGT+TGG	-	Chr2:51284940-51284959	MsG0280009394.01.T01:CDS	50.0%
	GGTACACCAAAGCTTGGACT+AGG	+	Chr2:51284847-51284866	None:intergenic	50.0%
	GTTCGTCCTAGTCCAAGCTT+TGG	-	Chr2:51284838-51284857	MsG0280009394.01.T01:CDS	50.0%
	CGCGTTCCGTGAGTATTCGT+CGG	-	Chr2:51284933-51284952	MsG0280009394.01.T01:CDS	55.0%
	GAAGGAGAGGGTTCTCCTTC+AGG	+	Chr2:51284986-51285005	None:intergenic	55.0%
	CGAATACTCACGGAACGCGC+CGG	+	Chr2:51284932-51284951	None:intergenic	60.0%
	GGAGAGGGTTCTCCTTCAGG+AGG	+	Chr2:51284983-51285002	None:intergenic	60.0%

Chromosome	Type	Strat	End	Strand	Name
Chr2	gene	51284797	51285042	51284797	ID=MsG0280009394.01;Name=MsG0280009394.01
Chr2	mRNA	51284797	51285042	51284797	ID=MsG0280009394.01.T01;Parent=MsG0280009394.01;Name=MsG0280009394.01.T01;_AED=0.01;_eAED=0.01;_QI=0\|-1\|0\|1\|-1\|0\|1\|0\|81
Chr2	exon	51284797	51285042	51284797	ID=MsG0280009394.01.T01:exon:31993;Parent=MsG0280009394.01.T01
Chr2	CDS	51284797	51285042	51284797	ID=MsG0280009394.01.T01:cds;Parent=MsG0280009394.01.T01

Gene Sequence	>MsG0280009394.01.T01 ATGGATAGTAGCTTATGTGACATGTTATTGAAGGTGGCTATGTTCGTCCTAGTCCAAGCTTTGGTGTACCTTATCCTTTCAAATTCATCAAACATATTCTCCAAGAACATCAAGAGATCAAACAGTTTCAAGCCGGCGCGTTCCGTGAGTATTCGTCGGATGTTGGCTTATCTCTCTGATTTTCCTCCTGAAGGAGAACCCTCTCCTTCTACAATGAGTCCTCAATCACAAGCAACTCAGAGTTAG
Protein sequence	>MsG0280009394.01.T01 MDSSLCDMLLKVAMFVLVQALVYLILSNSSNIFSKNIKRSNSFKPARSVSIRRMLAYLSDFPPEGEPSPSTMSPQSQATQS*