Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780040839.01.T01 | XP_013461666.1 | 94.915 | 59 | 1 | 1 | 2 | 58 | 86 | 144 | 3.31E-27 | 108 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780040839.01.T01 | A0A072V1Y3 | 94.915 | 59 | 1 | 1 | 2 | 58 | 86 | 144 | 1.58e-27 | 108 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180002234.01 | MsG0780040839.01 | 0.807448 | 5.030568e-50 | 2.802688e-47 |
| MsG0780040839.01 | MsG0880044788.01 | 0.814352 | 1.609886e-51 | 1.077688e-48 |
| MsG0380013489.01 | MsG0780040839.01 | 0.802068 | 6.684601e-49 | 3.243772e-46 |
| MsG0380013896.01 | MsG0780040839.01 | 0.820926 | 5.298807e-53 | 4.250020e-50 |
| MsG0580026631.01 | MsG0780040839.01 | 0.800598 | 1.336891e-48 | 6.250259e-46 |
| MsG0580029381.01 | MsG0780040839.01 | 0.806709 | 7.210903e-50 | 3.941060e-47 |
| MsG0280009248.01 | MsG0780040839.01 | 0.810683 | 1.020629e-50 | 6.192607e-48 |
| MsG0280009306.01 | MsG0780040839.01 | 0.800928 | 1.144903e-48 | 5.397871e-46 |
| MsG0780039282.01 | MsG0780040839.01 | 0.840815 | 6.925718e-58 | 9.981733e-55 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780040839.01.T01 | MTR_3g498865 | 94.915 | 59 | 1 | 1 | 2 | 58 | 86 | 144 | 4.00e-31 | 108 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 11 sgRNAs with CRISPR-Local
Find 17 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCAATTCGATCTTGGTCTTT+TGG | 0.187350 | 7:+83226751 | None:intergenic |
| TGGTAGCTGGAAAGTGAAAA+AGG | 0.224871 | 7:-83226777 | MsG0780040839.01.T01:CDS |
| AACACCGACGGTGTAATTAT+TGG | 0.331686 | 7:-83226797 | MsG0780040839.01.T01:CDS |
| CAAAAGACCAAGATCGAATT+GGG | 0.359194 | 7:-83226750 | MsG0780040839.01.T01:CDS |
| CCAAAAGACCAAGATCGAAT+TGG | 0.364115 | 7:-83226751 | MsG0780040839.01.T01:CDS |
| GAAGAAACCCAATTCGATCT+TGG | 0.472216 | 7:+83226743 | None:intergenic |
| AATAATTACACCGTCGGTGT+TGG | 0.505458 | 7:+83226799 | None:intergenic |
| GTTTCTTCGCGAATTGAAGA+AGG | 0.522515 | 7:-83226728 | MsG0780040839.01.T01:CDS |
| ACGGTGTAATTATTGGTAGC+TGG | 0.534282 | 7:-83226790 | MsG0780040839.01.T01:CDS |
| GCTACCAATAATTACACCGT+CGG | 0.667969 | 7:+83226793 | None:intergenic |
| GACGTAGAAACCAACACCGA+CGG | 0.675808 | 7:-83226809 | MsG0780040839.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AAACCAGTTCAAAAAAAATC+TGG | - | Chr7:83226811-83226830 | MsG0780040839.01.T01:CDS | 25.0% |
| !!! | AGATTTTGAAGGTGATAATT+CGG | - | Chr7:83226780-83226799 | MsG0780040839.01.T01:CDS | 25.0% |
| !!! | TAACCAGATTTTTTTTGAAC+TGG | + | Chr7:83226817-83226836 | None:intergenic | 25.0% |
| ! | GATTTGTATCGAGATTTTGA+AGG | - | Chr7:83226769-83226788 | MsG0780040839.01.T01:CDS | 30.0% |
| !! | GATTTTGAAGGTGATAATTC+GGG | - | Chr7:83226781-83226800 | MsG0780040839.01.T01:CDS | 30.0% |
| CAAAAGACCAAGATCGAATT+GGG | - | Chr7:83226702-83226721 | MsG0780040839.01.T01:CDS | 35.0% | |
| AACACCGACGGTGTAATTAT+TGG | - | Chr7:83226655-83226674 | MsG0780040839.01.T01:CDS | 40.0% | |
| AATAATTACACCGTCGGTGT+TGG | + | Chr7:83226656-83226675 | None:intergenic | 40.0% | |
| CCAAAAGACCAAGATCGAAT+TGG | - | Chr7:83226701-83226720 | MsG0780040839.01.T01:CDS | 40.0% | |
| GAAGAAACCCAATTCGATCT+TGG | + | Chr7:83226712-83226731 | None:intergenic | 40.0% | |
| GCTACCAATAATTACACCGT+CGG | + | Chr7:83226662-83226681 | None:intergenic | 40.0% | |
| GGTAGCTGGAAAGTGAAAAA+GGG | - | Chr7:83226676-83226695 | MsG0780040839.01.T01:CDS | 40.0% | |
| GTTTCTTCGCGAATTGAAGA+AGG | - | Chr7:83226724-83226743 | MsG0780040839.01.T01:CDS | 40.0% | |
| TGGTAGCTGGAAAGTGAAAA+AGG | - | Chr7:83226675-83226694 | MsG0780040839.01.T01:CDS | 40.0% | |
| ! | ACGGTGTAATTATTGGTAGC+TGG | - | Chr7:83226662-83226681 | MsG0780040839.01.T01:CDS | 40.0% |
| !! | CCAATTCGATCTTGGTCTTT+TGG | + | Chr7:83226704-83226723 | None:intergenic | 40.0% |
| GACGTAGAAACCAACACCGA+CGG | - | Chr7:83226643-83226662 | MsG0780040839.01.T01:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr7 | gene | 83226637 | 83226837 | 83226637 | ID=MsG0780040839.01;Name=MsG0780040839.01 |
| Chr7 | mRNA | 83226637 | 83226837 | 83226637 | ID=MsG0780040839.01.T01;Parent=MsG0780040839.01;Name=MsG0780040839.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|66 |
| Chr7 | exon | 83226637 | 83226837 | 83226637 | ID=MsG0780040839.01.T01:exon:2957;Parent=MsG0780040839.01.T01 |
| Chr7 | CDS | 83226637 | 83226837 | 83226637 | ID=MsG0780040839.01.T01:cds;Parent=MsG0780040839.01.T01 |
| Gene Sequence |
| Protein sequence |
