AlfalfaGEDB | MsG0780041087.01

M. sativa cultivar ZhongmuNo.1 / MsG0780041087.01

KEGG: -
GO: -
PFAM: PF03140

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0780041087.01.T01	XP_039683239.1	85.366	164	17	1	1	164	28	184	4.43E-91	283

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0780041087.01.T01	Q9SD53	39.073	151	87	4	17	164	32	180	6.59E-27	108

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0780041087.01.T01	A0A396H5T2	85.366	164	17	1	1	164	28	184	2.12e-91	283

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0780041087.01	MsG0780041301.01	0.805880	1.078070e-49	5.768841e-47
MsG0780041087.01	MsG0880042000.01	0.805627	1.218580e-49	6.478280e-47
MsG0480021564.01	MsG0780041087.01	0.807521	4.853667e-50	2.709308e-47
MsG0780039964.01	MsG0780041087.01	0.815819	7.605006e-52	5.298154e-49

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0780041087.01.T01	MTR_5g016570	40.580	138	77	2	32	164	102	239	7.97e-29	112
MsG0780041087.01.T01	MTR_3g079390	43.902	123	65	2	31	152	40	159	6.85e-25	100
MsG0780041087.01.T01	MTR_5g086230	34.194	155	93	5	19	164	16	170	4.07e-22	89.0
MsG0780041087.01.T01	MTR_6g009170	40.777	103	61	0	29	131	23	125	1.21e-21	91.3
MsG0780041087.01.T01	MTR_6g011050	34.615	156	84	4	11	156	11	158	1.48e-21	90.9
MsG0780041087.01.T01	MTR_6g009590	35.821	134	79	1	30	156	24	157	3.68e-21	89.7
MsG0780041087.01.T01	MTR_5g086340	34.641	153	93	3	19	164	20	172	5.94e-21	89.4
MsG0780041087.01.T01	MTR_6g009610	35.075	134	80	2	30	156	24	157	6.31e-21	89.4
MsG0780041087.01.T01	MTR_6g010920	39.604	101	61	0	31	131	25	125	1.06e-20	88.6
MsG0780041087.01.T01	MTR_6g011060	37.500	104	65	0	30	133	24	127	4.99e-20	86.7
MsG0780041087.01.T01	MTR_6g010890	39.423	104	61	1	30	131	24	127	6.15e-19	83.6
MsG0780041087.01.T01	MTR_0024s0080	39.806	103	61	1	32	133	26	128	1.91e-18	82.4
MsG0780041087.01.T01	MTR_7g067940	42.718	103	58	1	30	131	28	130	2.73e-18	82.0
MsG0780041087.01.T01	MTR_3g466740	36.054	147	83	4	14	149	9	155	6.19e-18	80.9
MsG0780041087.01.T01	MTR_7g068070	42.056	107	57	2	30	131	28	134	2.43e-17	79.3
MsG0780041087.01.T01	MTR_2g036320	34.507	142	79	3	30	164	66	200	4.09e-17	78.6
MsG0780041087.01.T01	MTR_3g107590	35.200	125	78	2	30	152	25	148	5.44e-17	78.2
MsG0780041087.01.T01	MTR_0067s0010	38.136	118	68	2	27	139	36	153	1.98e-16	76.6
MsG0780041087.01.T01	MTR_7g067660	38.182	110	60	2	30	131	15	124	5.79e-16	75.1
MsG0780041087.01.T01	MTR_6g010810	31.746	126	78	2	8	131	5	124	6.25e-16	75.1
MsG0780041087.01.T01	MTR_8g005265	35.252	139	82	4	31	164	27	162	6.70e-16	75.1
MsG0780041087.01.T01	MTR_2g041180	33.099	142	81	3	30	164	49	183	9.42e-16	74.7
MsG0780041087.01.T01	MTR_7g068160	39.450	109	58	3	30	130	35	143	3.07e-14	70.5
MsG0780041087.01.T01	MTR_8g005235	31.387	137	90	3	31	164	27	162	5.01e-14	69.7
MsG0780041087.01.T01	MTR_7g067970	38.318	107	61	2	30	131	28	134	1.73e-13	68.2
MsG0780041087.01.T01	MTR_5g087570	32.990	97	64	1	10	106	7	102	3.10e-13	63.5
MsG0780041087.01.T01	MTR_8g006525	31.159	138	89	4	31	164	28	163	8.24e-13	66.2
MsG0780041087.01.T01	MTR_8g005325	35.455	110	66	3	31	136	56	164	1.28e-12	65.5
MsG0780041087.01.T01	MTR_4g009340	26.429	140	85	3	8	132	8	144	1.39e-12	65.5
MsG0780041087.01.T01	MTR_0209s0060	33.333	105	66	3	31	131	29	133	1.63e-12	65.5
MsG0780041087.01.T01	MTR_0209s0030	33.333	105	66	3	31	131	29	133	1.63e-12	65.5
MsG0780041087.01.T01	MTR_4g009590	27.143	140	84	3	8	132	8	144	2.10e-12	65.1
MsG0780041087.01.T01	MTR_8g005295	27.215	158	92	4	30	164	35	192	2.35e-12	65.1
MsG0780041087.01.T01	MTR_8g005285	28.025	157	90	3	31	164	36	192	2.64e-12	64.7
MsG0780041087.01.T01	MTR_4g015290	35.514	107	61	3	30	135	27	126	3.25e-12	63.2
MsG0780041087.01.T01	MTR_1g040690	28.169	142	87	3	30	156	26	167	3.53e-12	64.3
MsG0780041087.01.T01	MTR_7g067990	36.697	109	61	3	30	130	23	131	4.78e-12	63.9
MsG0780041087.01.T01	MTR_7g011980	27.517	149	82	3	11	156	11	136	9.66e-12	62.8

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0780041087.01.T01	AT2G36430	62.585	147	54	1	19	164	33	179	1.81e-62	200
MsG0780041087.01.T01	AT3G44700	41.463	164	86	4	19	172	36	199	2.65e-35	123
MsG0780041087.01.T01	AT3G47210	44.203	138	76	1	28	164	90	227	4.45e-35	128
MsG0780041087.01.T01	AT5G22540	45.324	139	70	3	31	164	31	168	3.03e-34	125
MsG0780041087.01.T01	AT3G47250	37.647	170	99	4	2	164	29	198	1.49e-33	124
MsG0780041087.01.T01	AT3G47250	37.647	170	99	4	2	164	29	198	1.49e-33	124
MsG0780041087.01.T01	AT3G47250	37.647	170	99	4	2	164	29	198	1.49e-33	124
MsG0780041087.01.T01	AT5G22560	40.523	153	73	3	30	164	46	198	3.26e-31	118
MsG0780041087.01.T01	AT3G44710	39.474	152	86	3	19	164	35	186	1.72e-29	113
MsG0780041087.01.T01	AT5G22550	40.000	140	78	3	31	164	44	183	2.45e-29	113
MsG0780041087.01.T01	AT3G50170	39.706	136	79	1	32	164	114	249	2.28e-28	109
MsG0780041087.01.T01	AT3G50170	39.706	136	79	1	32	164	114	249	4.97e-28	109
MsG0780041087.01.T01	AT3G50160	32.934	167	97	2	1	164	86	240	5.48e-28	109
MsG0780041087.01.T01	AT3G47200	39.073	151	87	4	17	164	32	180	6.72e-28	108
MsG0780041087.01.T01	AT3G47200	39.073	151	87	4	17	164	32	180	6.72e-28	108
MsG0780041087.01.T01	AT3G50120	38.235	136	81	1	32	164	104	239	7.96e-28	109
MsG0780041087.01.T01	AT3G50130	38.235	136	81	1	32	164	140	275	1.82e-27	108
MsG0780041087.01.T01	AT3G50150	34.146	164	92	3	5	164	76	227	5.69e-27	106
MsG0780041087.01.T01	AT4G31980	48.515	101	52	0	31	131	293	393	6.16e-27	107
MsG0780041087.01.T01	AT3G50150	34.146	164	92	3	5	164	76	227	7.43e-27	106
MsG0780041087.01.T01	AT3G50190	34.194	155	98	2	32	182	97	251	1.60e-26	103
MsG0780041087.01.T01	AT3G50190	36.029	136	84	1	32	164	97	232	2.33e-26	103
MsG0780041087.01.T01	AT3G50190	36.029	136	84	1	32	164	97	232	2.33e-26	103
MsG0780041087.01.T01	AT3G44700	36.076	158	76	4	19	172	36	172	7.18e-26	98.6
MsG0780041087.01.T01	AT5G11290	44.118	102	57	0	30	131	32	133	9.30e-26	102
MsG0780041087.01.T01	AT3G50190	36.029	136	84	1	32	164	97	232	1.03e-25	103
MsG0780041087.01.T01	AT3G50140	35.294	136	85	1	32	164	114	249	1.34e-25	102
MsG0780041087.01.T01	AT3G50180	38.235	136	81	1	32	164	179	314	1.79e-25	102
MsG0780041087.01.T01	AT3G50140	35.294	136	85	1	32	164	114	249	3.70e-25	101
MsG0780041087.01.T01	AT5G22550	51.786	56	25	1	31	84	44	99	1.02e-12	65.9
MsG0780041087.01.T01	AT5G11290	42.188	64	37	0	68	131	1	64	9.78e-12	62.8

Find 29 sgRNAs with CRISPR-Local

Find 36 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GCGGATTTGCTTAGTAATTT+TGG	0.067484	7:+86341236	None:intergenic
GGCTTCCAAAACCTCTTCTA+AGG	0.272835	7:+86341035	None:intergenic
ATTACCCTTAGAAGAGGTTT+TGG	0.319735	7:-86341040	MsG0780041087.01.T01:CDS
GAACATAAATATCGTTATCT+TGG	0.354708	7:-86341084	MsG0780041087.01.T01:CDS
AATATCTTGTTGCATCTTCA+AGG	0.415156	7:-86341208	MsG0780041087.01.T01:CDS
TTCGTGTGAATCAAGTTGAA+TGG	0.452910	7:+86340969	None:intergenic
ACACGAATTCGTTGAAATGA+TGG	0.453940	7:-86340953	MsG0780041087.01.T01:CDS
GTTGAAATGATGGTACTTGA+TGG	0.462276	7:-86340943	MsG0780041087.01.T01:CDS
TCCACGATGGTAAGGTCCTA+TGG	0.474312	7:+86341131	None:intergenic
ATCCACTTGTTAACATGGCA+TGG	0.502614	7:-86340861	MsG0780041087.01.T01:CDS
AAGTGGATCATCAACTTCAA+AGG	0.512139	7:+86340876	None:intergenic
CGAGGTTGTCCACGATGGTA+AGG	0.515482	7:+86341123	None:intergenic
AGTGGATCATCAACTTCAAA+GGG	0.524431	7:+86340877	None:intergenic
ATCCATGCCATGTTAACAAG+TGG	0.530059	7:+86340859	None:intergenic
AACACAATTACCCTTAGAAG+AGG	0.535365	7:-86341046	MsG0780041087.01.T01:CDS
TTTGCTTCAACGAAGGTTTG+TGG	0.536234	7:+86341183	None:intergenic
AAGCGTTGAAGAACAAAGAA+AGG	0.541868	7:+86340791	None:intergenic
GCTTCCAAAACCTCTTCTAA+GGG	0.545818	7:+86341036	None:intergenic
TTTAACGTTTGCTTCAACGA+AGG	0.547855	7:+86341176	None:intergenic
TGATGATCCACTTGTTAACA+TGG	0.571823	7:-86340866	MsG0780041087.01.T01:CDS
ACCACCAAATGAAAATTCAA+CGG	0.589532	7:-86340753	MsG0780041087.01.T01:CDS
CACCCTCGTATAGTTTCCAT+AGG	0.628917	7:-86341147	MsG0780041087.01.T01:CDS
AAATTACTAAGCAAATCCGC+AGG	0.640596	7:-86341233	MsG0780041087.01.T01:CDS
TCTTCGATCATGTTGAGACG+AGG	0.666022	7:+86341105	None:intergenic
GTCCTATGGAAACTATACGA+GGG	0.676624	7:+86341145	None:intergenic
TCCATAGGACCTTACCATCG+TGG	0.683623	7:-86341132	MsG0780041087.01.T01:CDS
CTATGGAAACTATACGAGGG+TGG	0.693319	7:+86341148	None:intergenic
GGTCCTATGGAAACTATACG+AGG	0.715116	7:+86341144	None:intergenic
TGAGACGAGGTTGTCCACGA+TGG	0.728331	7:+86341118	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AAGAAATCTCTATAAAAAAA+TGG	+	Chr7:86341212-86341231	None:intergenic	15.0%
!	GAACATAAATATCGTTATCT+TGG	-	Chr7:86340957-86340976	MsG0780041087.01.T01:CDS	25.0%
	AATATCTTGTTGCATCTTCA+AGG	-	Chr7:86340833-86340852	MsG0780041087.01.T01:CDS	30.0%
	ACCACCAAATGAAAATTCAA+CGG	-	Chr7:86341288-86341307	MsG0780041087.01.T01:CDS	30.0%
!	TTCGTTTTCTAATGGTGATA+TGG	+	Chr7:86341030-86341049	None:intergenic	30.0%
!!	CGGATTTGCTTAGTAATTTT+GGG	+	Chr7:86340807-86340826	None:intergenic	30.0%
	AAATTACTAAGCAAATCCGC+AGG	-	Chr7:86340808-86340827	MsG0780041087.01.T01:CDS	35.0%
	AACACAATTACCCTTAGAAG+AGG	-	Chr7:86340995-86341014	MsG0780041087.01.T01:CDS	35.0%
	AAGTGGATCATCAACTTCAA+AGG	+	Chr7:86341168-86341187	None:intergenic	35.0%
	ACACGAATTCGTTGAAATGA+TGG	-	Chr7:86341088-86341107	MsG0780041087.01.T01:CDS	35.0%
	AGTGGATCATCAACTTCAAA+GGG	+	Chr7:86341167-86341186	None:intergenic	35.0%
	GTTGAAATGATGGTACTTGA+TGG	-	Chr7:86341098-86341117	MsG0780041087.01.T01:CDS	35.0%
	TTTAACGTTTGCTTCAACGA+AGG	+	Chr7:86340868-86340887	None:intergenic	35.0%
!	ACCGTTGAATTTTCATTTGG+TGG	+	Chr7:86341292-86341311	None:intergenic	35.0%
!	ATTACCCTTAGAAGAGGTTT+TGG	-	Chr7:86341001-86341020	MsG0780041087.01.T01:CDS	35.0%
!	TGATGATCCACTTGTTAACA+TGG	-	Chr7:86341175-86341194	MsG0780041087.01.T01:CDS	35.0%
!	TGCAACAAGATATTTTCCTG+CGG	+	Chr7:86340827-86340846	None:intergenic	35.0%
!!	AAGCGTTGAAGAACAAAGAA+AGG	+	Chr7:86341253-86341272	None:intergenic	35.0%
!!	GCGGATTTGCTTAGTAATTT+TGG	+	Chr7:86340808-86340827	None:intergenic	35.0%
!!	TCTCTAGCTTCGTTTTCTAA+TGG	+	Chr7:86341038-86341057	None:intergenic	35.0%
!!	TTCGTGTGAATCAAGTTGAA+TGG	+	Chr7:86341075-86341094	None:intergenic	35.0%
!!!	AACTTTTTCGCAAAGTTGCA+AGG	-	Chr7:86341135-86341154	MsG0780041087.01.T01:CDS	35.0%
	ATCCATGCCATGTTAACAAG+TGG	+	Chr7:86341185-86341204	None:intergenic	40.0%
	GCTTCCAAAACCTCTTCTAA+GGG	+	Chr7:86341008-86341027	None:intergenic	40.0%
	GTCCTATGGAAACTATACGA+GGG	+	Chr7:86340899-86340918	None:intergenic	40.0%
	TTTGCTTCAACGAAGGTTTG+TGG	+	Chr7:86340861-86340880	None:intergenic	40.0%
!	ATCCACTTGTTAACATGGCA+TGG	-	Chr7:86341180-86341199	MsG0780041087.01.T01:CDS	40.0%
	CACCCTCGTATAGTTTCCAT+AGG	-	Chr7:86340894-86340913	MsG0780041087.01.T01:CDS	45.0%
	CTATGGAAACTATACGAGGG+TGG	+	Chr7:86340896-86340915	None:intergenic	45.0%
	GGCTTCCAAAACCTCTTCTA+AGG	+	Chr7:86341009-86341028	None:intergenic	45.0%
	GGTCCTATGGAAACTATACG+AGG	+	Chr7:86340900-86340919	None:intergenic	45.0%
	TCTTCGATCATGTTGAGACG+AGG	+	Chr7:86340939-86340958	None:intergenic	45.0%
	TCCACGATGGTAAGGTCCTA+TGG	+	Chr7:86340913-86340932	None:intergenic	50.0%
	TCCATAGGACCTTACCATCG+TGG	-	Chr7:86340909-86340928	MsG0780041087.01.T01:CDS	50.0%
	CGAGGTTGTCCACGATGGTA+AGG	+	Chr7:86340921-86340940	None:intergenic	55.0%
	TGAGACGAGGTTGTCCACGA+TGG	+	Chr7:86340926-86340945	None:intergenic	55.0%

Chromosome	Type	Strat	End	Strand	Name
Chr7	gene	86340750	86341313	86340750	ID=MsG0780041087.01;Name=MsG0780041087.01
Chr7	mRNA	86340750	86341313	86340750	ID=MsG0780041087.01.T01;Parent=MsG0780041087.01;Name=MsG0780041087.01.T01;_AED=0.33;_eAED=0.47;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|187
Chr7	exon	86340750	86341313	86340750	ID=MsG0780041087.01.T01:exon:52025;Parent=MsG0780041087.01.T01
Chr7	CDS	86340750	86341313	86340750	ID=MsG0780041087.01.T01:cds;Parent=MsG0780041087.01.T01

Gene Sequence

>MsG0780041087.01.T01

CTGCAAAATAGACAACGAAAGATTAGCTTCAATGCAACAAAAAATTTCAGAAAACCCAAAATTACTAAGCAAATCCGCAGGAAAATATCTTGTTGCATCTTCAAGGTACCACAAACCTTCGTTGAAGCAAACGTTAAAGCATACCACCCTCGTATAGTTTCCATAGGACCTTACCATCGTGGACAACCTCGTCTCAACATGATCGAAGAACATAAATATCGTTATCTTGGTTCTCTTCTGACAAGAACACAATTACCCTTAGAAGAGGTTTTGGAAGCCATATCACCATTAGAAAACGAAGCTAGAGAGTGTTACTCTGAAACCATTCAACTTGATTCACACGAATTCGTTGAAATGATGGTACTTGATGGTTGTTTCATCATTGAACTTTTTCGCAAAGTTGCAAGGTTAGTACCCTTTGAAGTTGATGATCCACTTGTTAACATGGCATGGATTTTACCATTTTTTTATAGAGATTTCTTAAGCTTGAAAATCAAATACCTTTCTTTGTTCTTCAACGCTTGTTCGAAATTTCAAAACCACCAAATGAAAATTCAACGGTAA

Protein sequence

>MsG0780041087.01.T01

LQNRQRKISFNATKNFRKPKITKQIRRKISCCIFKVPQTFVEANVKAYHPRIVSIGPYHRGQPRLNMIEEHKYRYLGSLLTRTQLPLEEVLEAISPLENEARECYSETIQLDSHEFVEMMVLDGCFIIELFRKVARLVPFEVDDPLVNMAWILPFFYRDFLSLKIKYLSLFFNACSKFQNHQMKIQR*