Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780041533.01.T01 | XP_003626248.1 | 100 | 105 | 0 | 0 | 1 | 105 | 22 | 126 | 4.95E-73 | 223 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780041533.01.T01 | Q8L9P8 | 84.211 | 76 | 12 | 0 | 30 | 105 | 40 | 115 | 1.23E-42 | 138 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780041533.01.T01 | A2Q327 | 100.000 | 105 | 0 | 0 | 1 | 105 | 22 | 126 | 2.36e-73 | 223 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0780041293.01 | MsG0780041533.01 | 0.815115 | 1.090482e-51 | 7.451695e-49 |
| MsG0480021042.01 | MsG0780041533.01 | 0.832806 | 7.661532e-56 | 8.657463e-53 |
| MsG0480021987.01 | MsG0780041533.01 | 0.820890 | 5.399950e-53 | 4.326835e-50 |
| MsG0380016153.01 | MsG0780041533.01 | 0.808322 | 3.278651e-50 | 1.869033e-47 |
| MsG0280008854.01 | MsG0780041533.01 | 0.831669 | 1.464647e-55 | 1.600337e-52 |
| MsG0780039441.01 | MsG0780041533.01 | 0.850175 | 2.012414e-60 | 3.908150e-57 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780041533.01.T01 | MTR_7g113080 | 100.000 | 105 | 0 | 0 | 1 | 105 | 22 | 126 | 6.00e-77 | 223 |
| MsG0780041533.01.T01 | MTR_4g119860 | 62.617 | 107 | 33 | 2 | 2 | 105 | 23 | 125 | 5.30e-40 | 129 |
| MsG0780041533.01.T01 | MTR_7g108600 | 56.481 | 108 | 40 | 3 | 1 | 106 | 15 | 117 | 4.40e-35 | 116 |
| MsG0780041533.01.T01 | MTR_3g084350 | 53.247 | 77 | 32 | 3 | 29 | 104 | 53 | 126 | 4.53e-20 | 79.3 |
| MsG0780041533.01.T01 | MTR_4g088685 | 56.250 | 64 | 26 | 1 | 43 | 104 | 44 | 107 | 2.54e-19 | 76.6 |
| MsG0780041533.01.T01 | MTR_6g060260 | 48.485 | 66 | 29 | 1 | 39 | 104 | 41 | 101 | 1.28e-15 | 67.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780041533.01.T01 | AT4G15800 | 84.211 | 76 | 12 | 0 | 30 | 105 | 40 | 115 | 1.26e-43 | 138 |
| MsG0780041533.01.T01 | AT1G02900 | 62.617 | 107 | 36 | 2 | 1 | 106 | 17 | 120 | 1.19e-40 | 130 |
| MsG0780041533.01.T01 | AT3G16570 | 65.556 | 90 | 17 | 2 | 30 | 105 | 47 | 136 | 3.17e-33 | 112 |
| MsG0780041533.01.T01 | AT3G16570 | 65.556 | 90 | 17 | 2 | 30 | 105 | 47 | 136 | 3.17e-33 | 112 |
| MsG0780041533.01.T01 | AT3G16570 | 65.556 | 90 | 17 | 2 | 30 | 105 | 47 | 136 | 3.17e-33 | 112 |
| MsG0780041533.01.T01 | AT3G05490 | 81.034 | 58 | 11 | 0 | 48 | 105 | 61 | 118 | 3.52e-30 | 104 |
| MsG0780041533.01.T01 | AT2G33775 | 57.971 | 69 | 28 | 1 | 37 | 104 | 37 | 105 | 4.99e-23 | 85.9 |
| MsG0780041533.01.T01 | AT1G28270 | 55.072 | 69 | 30 | 1 | 37 | 104 | 37 | 105 | 2.18e-19 | 76.6 |
| MsG0780041533.01.T01 | AT4G13950 | 57.812 | 64 | 25 | 2 | 42 | 104 | 42 | 104 | 5.44e-18 | 73.2 |
| MsG0780041533.01.T01 | AT3G23805 | 60.714 | 56 | 20 | 2 | 50 | 104 | 55 | 109 | 1.10e-15 | 67.4 |
| MsG0780041533.01.T01 | AT5G67070 | 51.282 | 78 | 27 | 5 | 39 | 105 | 51 | 128 | 3.45e-12 | 58.9 |
Find 29 sgRNAs with CRISPR-Local
Find 30 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCACTGCATCCACGGCTGTA+AGG | 0.445471 | 7:+92099531 | None:intergenic |
| AGTTTGATAATGAAATCAAC+AGG | 0.461525 | 7:-92099668 | MsG0780041533.01.T01:CDS |
| CGCACCATAGCTGATGTACT+TGG | 0.465658 | 7:+92099629 | None:intergenic |
| CACTGCATCCACGGCTGTAA+GGG | 0.481328 | 7:+92099532 | None:intergenic |
| CAATTGCAGACCCGGTGCTC+AGG | 0.488139 | 7:-92099559 | MsG0780041533.01.T01:CDS |
| CCTCCAGCTGCATCTACTGT+TGG | 0.503199 | 7:+92099792 | None:intergenic |
| GCAGCTGGAGGATTCGAACT+TGG | 0.512453 | 7:-92099780 | MsG0780041533.01.T01:CDS |
| GAAGCACCACGGCGAGAACA+AGG | 0.526826 | 7:+92099588 | None:intergenic |
| GTAAGGGTTAGCCTGAGCAC+CGG | 0.526971 | 7:+92099548 | None:intergenic |
| ATCAGCGATGGAACCTTCGC+AGG | 0.533132 | 7:+92099722 | None:intergenic |
| TCTCCAACAGTAGATGCAGC+TGG | 0.538924 | 7:-92099795 | MsG0780041533.01.T01:CDS |
| TCTTACTACAATTGCAGACC+CGG | 0.540294 | 7:-92099567 | MsG0780041533.01.T01:CDS |
| AGGATTCGAACTTGGAGGAA+TGG | 0.547500 | 7:-92099772 | MsG0780041533.01.T01:CDS |
| GCTGGAGGATTCGAACTTGG+AGG | 0.550332 | 7:-92099777 | MsG0780041533.01.T01:CDS |
| TCGAACTTGGAGGAATGGAA+TGG | 0.552877 | 7:-92099767 | MsG0780041533.01.T01:CDS |
| TCAGCTATGGTGCGCTTCAG+AGG | 0.563424 | 7:-92099620 | MsG0780041533.01.T01:CDS |
| GCAGTGCTATTACGAGGTGC+AGG | 0.566649 | 7:-92099515 | MsG0780041533.01.T01:CDS |
| TCGTAATAGCACTGCATCCA+CGG | 0.577260 | 7:+92099523 | None:intergenic |
| TAAGGGTTAGCCTGAGCACC+GGG | 0.580885 | 7:+92099549 | None:intergenic |
| TTGTAACATGCAATCAGCGA+TGG | 0.586357 | 7:+92099710 | None:intergenic |
| CAGGCTAACCCTTACAGCCG+TGG | 0.595858 | 7:-92099540 | MsG0780041533.01.T01:CDS |
| ACGACCAAGTACATCAGCTA+TGG | 0.604031 | 7:-92099633 | MsG0780041533.01.T01:CDS |
| AGCGATGGAACCTTCGCAGG+TGG | 0.625016 | 7:+92099725 | None:intergenic |
| AAGCACCACGGCGAGAACAA+GGG | 0.626487 | 7:+92099589 | None:intergenic |
| ACTGTCCCTTGTTCTCGCCG+TGG | 0.647098 | 7:-92099594 | MsG0780041533.01.T01:CDS |
| CCAACAGTAGATGCAGCTGG+AGG | 0.701563 | 7:-92099792 | MsG0780041533.01.T01:CDS |
| GCTGATTGCATGTTACAACA+AGG | 0.712911 | 7:-92099705 | MsG0780041533.01.T01:CDS |
| GTGGATGCAGTGCTATTACG+AGG | 0.733772 | 7:-92099521 | MsG0780041533.01.T01:CDS |
| AATTGTAGTAAGAAGCACCA+CGG | 0.751980 | 7:+92099577 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AGTTTGATAATGAAATCAAC+AGG | - | Chr7:92099648-92099667 | MsG0780041533.01.T01:CDS | 25.0% |
| !! | AATTGTAGTAAGAAGCACCA+CGG | + | Chr7:92099742-92099761 | None:intergenic | 35.0% |
| GCTGATTGCATGTTACAACA+AGG | - | Chr7:92099611-92099630 | MsG0780041533.01.T01:CDS | 40.0% | |
| TCTTACTACAATTGCAGACC+CGG | - | Chr7:92099749-92099768 | MsG0780041533.01.T01:CDS | 40.0% | |
| TTGTAACATGCAATCAGCGA+TGG | + | Chr7:92099609-92099628 | None:intergenic | 40.0% | |
| ACGACCAAGTACATCAGCTA+TGG | - | Chr7:92099683-92099702 | MsG0780041533.01.T01:CDS | 45.0% | |
| AGGATTCGAACTTGGAGGAA+TGG | - | Chr7:92099544-92099563 | MsG0780041533.01.T01:CDS | 45.0% | |
| TCGAACTTGGAGGAATGGAA+TGG | - | Chr7:92099549-92099568 | MsG0780041533.01.T01:CDS | 45.0% | |
| TCGTAATAGCACTGCATCCA+CGG | + | Chr7:92099796-92099815 | None:intergenic | 45.0% | |
| ACAAAAACAGCCACCTGCGA+AGG | - | Chr7:92099581-92099600 | MsG0780041533.01.T01:CDS | 50.0% | |
| CGCACCATAGCTGATGTACT+TGG | + | Chr7:92099690-92099709 | None:intergenic | 50.0% | |
| TCTCCAACAGTAGATGCAGC+TGG | - | Chr7:92099521-92099540 | MsG0780041533.01.T01:CDS | 50.0% | |
| ! | GTGGATGCAGTGCTATTACG+AGG | - | Chr7:92099795-92099814 | MsG0780041533.01.T01:CDS | 50.0% |
| AAGCACCACGGCGAGAACAA+GGG | + | Chr7:92099730-92099749 | None:intergenic | 55.0% | |
| ATCAGCGATGGAACCTTCGC+AGG | + | Chr7:92099597-92099616 | None:intergenic | 55.0% | |
| CACTGCATCCACGGCTGTAA+GGG | + | Chr7:92099787-92099806 | None:intergenic | 55.0% | |
| CCAACAGTAGATGCAGCTGG+AGG | - | Chr7:92099524-92099543 | MsG0780041533.01.T01:CDS | 55.0% | |
| CCTCCAGCTGCATCTACTGT+TGG | + | Chr7:92099527-92099546 | None:intergenic | 55.0% | |
| GCAGCTGGAGGATTCGAACT+TGG | - | Chr7:92099536-92099555 | MsG0780041533.01.T01:CDS | 55.0% | |
| GCTGGAGGATTCGAACTTGG+AGG | - | Chr7:92099539-92099558 | MsG0780041533.01.T01:CDS | 55.0% | |
| TCAGCTATGGTGCGCTTCAG+AGG | - | Chr7:92099696-92099715 | MsG0780041533.01.T01:CDS | 55.0% | |
| ! | GCAGTGCTATTACGAGGTGC+AGG | - | Chr7:92099801-92099820 | MsG0780041533.01.T01:CDS | 55.0% |
| !! | GTAAGGGTTAGCCTGAGCAC+CGG | + | Chr7:92099771-92099790 | None:intergenic | 55.0% |
| !! | TAAGGGTTAGCCTGAGCACC+GGG | + | Chr7:92099770-92099789 | None:intergenic | 55.0% |
| ACTGTCCCTTGTTCTCGCCG+TGG | - | Chr7:92099722-92099741 | MsG0780041533.01.T01:CDS | 60.0% | |
| AGCGATGGAACCTTCGCAGG+TGG | + | Chr7:92099594-92099613 | None:intergenic | 60.0% | |
| CAATTGCAGACCCGGTGCTC+AGG | - | Chr7:92099757-92099776 | MsG0780041533.01.T01:CDS | 60.0% | |
| CAGGCTAACCCTTACAGCCG+TGG | - | Chr7:92099776-92099795 | MsG0780041533.01.T01:CDS | 60.0% | |
| GCACTGCATCCACGGCTGTA+AGG | + | Chr7:92099788-92099807 | None:intergenic | 60.0% | |
| ! | GAAGCACCACGGCGAGAACA+AGG | + | Chr7:92099731-92099750 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr7 | gene | 92099509 | 92099829 | 92099509 | ID=MsG0780041533.01;Name=MsG0780041533.01 |
| Chr7 | mRNA | 92099509 | 92099829 | 92099509 | ID=MsG0780041533.01.T01;Parent=MsG0780041533.01;Name=MsG0780041533.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|106 |
| Chr7 | exon | 92099509 | 92099829 | 92099509 | ID=MsG0780041533.01.T01:exon:19322;Parent=MsG0780041533.01.T01 |
| Chr7 | CDS | 92099509 | 92099829 | 92099509 | ID=MsG0780041533.01.T01:cds;Parent=MsG0780041533.01.T01 |
| Gene Sequence |
| Protein sequence |
