AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0780041293.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780041293.01.T01 MTR_7g108675 74.786 234 19 4 1 194 233 466 8.70e-116 337
MsG0780041293.01.T01 MTR_4g119880 67.949 234 35 4 1 194 221 454 3.05e-108 317
MsG0780041293.01.T01 MTR_8g058553 66.484 182 21 4 1 142 233 414 6.26e-75 234
MsG0780041293.01.T01 MTR_4g108940 46.983 232 83 3 1 192 217 448 1.76e-71 223
MsG0780041293.01.T01 MTR_3g037340 47.727 220 75 2 1 180 222 441 7.50e-70 219
MsG0780041293.01.T01 MTR_4g097230 55.556 117 48 2 1 113 180 296 4.87e-36 130
MsG0780041293.01.T01 MTR_5g015260 53.000 100 47 0 1 100 201 300 1.88e-33 124
MsG0780041293.01.T01 MTR_4g097240 53.000 100 47 0 1 100 195 294 5.66e-31 117
MsG0780041293.01.T01 MTR_4g108950 44.944 89 41 2 65 151 1 83 8.90e-16 69.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780041293.01.T01 AT4G02500 67.521 234 36 4 1 194 227 460 1.05e-105 311
MsG0780041293.01.T01 AT3G62720 64.103 234 44 4 1 194 226 459 1.23e-99 296
MsG0780041293.01.T01 AT3G62720 64.103 234 44 4 1 194 226 459 1.23e-99 296
MsG0780041293.01.T01 AT5G07720 47.414 232 82 3 1 192 226 457 1.38e-70 221
MsG0780041293.01.T01 AT1G74380 48.636 220 73 3 1 180 227 446 2.87e-70 220
MsG0780041293.01.T01 AT1G18690 47.273 220 76 3 1 180 285 504 4.10e-68 216
MsG0780041293.01.T01 AT1G18690 47.273 220 76 3 1 180 285 504 4.10e-68 216
MsG0780041293.01.T01 AT1G18690 47.273 220 76 3 1 180 285 504 4.10e-68 216
MsG0780041293.01.T01 AT1G18690 47.273 220 76 3 1 180 285 504 4.10e-68 216
MsG0780041293.01.T01 AT2G22900 47.170 106 56 0 1 106 200 305 1.57e-30 116
MsG0780041293.01.T01 AT4G37690 48.000 100 52 0 1 100 183 282 3.93e-29 112

Find 52 sgRNAs with CRISPR-Local

Find 80 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATCAAATATTGCAGATTTA+TGG 0.107608 7:-89245269 MsG0780041293.01.T01:CDS
TGATGCTATGTTTACGGATA+TGG 0.232667 7:-89245805 MsG0780041293.01.T01:CDS
GTAGGAGAGTTAGGAGAATA+AGG 0.319874 7:-89245223 MsG0780041293.01.T01:CDS
ATGGATAGAGCTTTCAATTT+CGG 0.322531 7:-89245296 MsG0780041293.01.T01:CDS
TGGGACCGAAGGGGAAAGTT+AGG 0.323142 7:-89245620 MsG0780041293.01.T01:CDS
CATTGGTTAGTAGGAGAGTT+AGG 0.330087 7:-89245232 MsG0780041293.01.T01:CDS
CTCATAAGTCATTGGTTAGT+AGG 0.331750 7:-89245241 MsG0780041293.01.T01:CDS
GAGGTATAAAGATTCAAATT+TGG 0.351394 7:-89245763 MsG0780041293.01.T01:CDS
ATTGGATATTCTTGATGCTT+GGG 0.353018 7:-89245649 MsG0780041293.01.T01:CDS
AATTGGATTGGGTTGAATAC+TGG 0.358814 7:-89245702 MsG0780041293.01.T01:CDS
CATTGGATATTCTTGATGCT+TGG 0.377065 7:-89245650 MsG0780041293.01.T01:CDS
TGATCATCTGCTTCGAAAAC+CGG 0.408172 7:+89245555 None:intergenic
TGGATTCACTCATAAGTCAT+TGG 0.408588 7:-89245249 MsG0780041293.01.T01:CDS
GGATAGTGATGCTATGTTTA+CGG 0.413937 7:-89245811 MsG0780041293.01.T01:CDS
GAAGGAAAAGTGGGGTGATA+AGG 0.433266 7:-89245503 MsG0780041293.01.T01:CDS
GATAAGGTTTATCTTGAGAA+TGG 0.437845 7:-89245487 MsG0780041293.01.T01:CDS
CTTGAGGTTAAAGATGAACT+TGG 0.442102 7:-89245182 MsG0780041293.01.T01:CDS
TCATCCCTAACTTTCCCCTT+CGG 0.442497 7:+89245615 None:intergenic
CTATGGTTTATTTGTGGCCA+AGG 0.451537 7:-89245527 MsG0780041293.01.T01:CDS
GGGAAAGTTAGGGATGAAGC+AGG 0.456571 7:-89245609 MsG0780041293.01.T01:CDS
AATCTGCTATGGTTTATTTG+TGG 0.463425 7:-89245533 MsG0780041293.01.T01:CDS
TATGATGAAAAGAATTGGAT+TGG 0.467866 7:-89245714 MsG0780041293.01.T01:CDS
TCTTGATGCTTGGGCACCAA+TGG 0.469863 7:-89245640 MsG0780041293.01.T01:CDS
ACTTTCCCCTTCGGTCCCAT+TGG 0.471564 7:+89245624 None:intergenic
TGGTGTATGATGAAAAGAAT+TGG 0.476382 7:-89245719 MsG0780041293.01.T01:CDS
GAGAATGGTTATTACTTGCA+TGG 0.490493 7:-89245472 MsG0780041293.01.T01:intron
CTTGATGCTTGGGCACCAAT+GGG 0.494441 7:-89245639 MsG0780041293.01.T01:CDS
CAAATTTGGTGATGCATGGT+TGG 0.496007 7:-89245749 MsG0780041293.01.T01:CDS
AGTTCATCTTTAACCTCAAG+AGG 0.497545 7:+89245185 None:intergenic
TTACGCGCGAACTCAAAGAT+AGG 0.499667 7:-89245578 MsG0780041293.01.T01:CDS
TGCTTCAAGCATCTCTCCAC+AGG 0.506143 7:+89245320 None:intergenic
TTGGGCACCAATGGGACCGA+AGG 0.511361 7:-89245631 MsG0780041293.01.T01:CDS
TTTATTTGTGGCCAAGGAGA+AGG 0.518143 7:-89245521 MsG0780041293.01.T01:CDS
TGGGCACCAATGGGACCGAA+GGG 0.520017 7:-89245630 MsG0780041293.01.T01:CDS
GAGGAACTGTCAGTGGTCAT+TGG 0.527929 7:-89245667 MsG0780041293.01.T01:CDS
GGGACCGAAGGGGAAAGTTA+GGG 0.533356 7:-89245619 MsG0780041293.01.T01:CDS
ATGATGAAAAGAATTGGATT+GGG 0.548297 7:-89245713 MsG0780041293.01.T01:CDS
GCGCGAACTCAAAGATAGGC+CGG 0.551019 7:-89245574 MsG0780041293.01.T01:CDS
GGAGAGATGCTTGAAGCAGA+TGG 0.564442 7:-89245315 MsG0780041293.01.T01:CDS
GGAAAGTTAGGGATGAAGCA+GGG 0.572682 7:-89245608 MsG0780041293.01.T01:CDS
GATTCAAATTTGGTGATGCA+TGG 0.574422 7:-89245753 MsG0780041293.01.T01:CDS
AGCAGATGATCAATCTGCTA+TGG 0.575705 7:-89245544 MsG0780041293.01.T01:CDS
ACCTCAACAGCTTTAAAAGT+CGG 0.576971 7:+89245149 None:intergenic
GGCCAAGGAGAAGGAAAAGT+GGG 0.582423 7:-89245512 MsG0780041293.01.T01:CDS
TGGCCAAGGAGAAGGAAAAG+TGG 0.598754 7:-89245513 MsG0780041293.01.T01:CDS
TGAGACTAGTAATCCTCTTG+AGG 0.598964 7:-89245198 MsG0780041293.01.T01:CDS
CAACAGCTTTAAAAGTCGGA+TGG 0.599198 7:+89245153 None:intergenic
GAATCTTTATACCTCTCCCA+CGG 0.600071 7:+89245771 None:intergenic
GATGCATGGTTGGAATGAGA+TGG 0.650662 7:-89245739 MsG0780041293.01.T01:CDS
GGGCACCAATGGGACCGAAG+GGG 0.652159 7:-89245629 MsG0780041293.01.T01:CDS
GAAGTTTGGTGATTATCCTG+TGG 0.716211 7:-89245336 MsG0780041293.01.T01:intron
GCCAAGGAGAAGGAAAAGTG+GGG 0.724519 7:-89245511 MsG0780041293.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATCAAATATTGCAGATTTA+TGG - Chr7:89245677-89245696 MsG0780041293.01.T01:CDS 20.0%
! ATGATGAAAAGAATTGGATT+GGG - Chr7:89245233-89245252 MsG0780041293.01.T01:CDS 25.0%
! GAGGTATAAAGATTCAAATT+TGG - Chr7:89245183-89245202 MsG0780041293.01.T01:CDS 25.0%
! TATGATGAAAAGAATTGGAT+TGG - Chr7:89245232-89245251 MsG0780041293.01.T01:CDS 25.0%
AATCTGCTATGGTTTATTTG+TGG - Chr7:89245413-89245432 MsG0780041293.01.T01:intron 30.0%
ATGATTGAGAATTATCATCC+TGG - Chr7:89245531-89245550 MsG0780041293.01.T01:CDS 30.0%
ATGGATAGAGCTTTCAATTT+CGG - Chr7:89245650-89245669 MsG0780041293.01.T01:CDS 30.0%
ATTGGATATTCTTGATGCTT+GGG - Chr7:89245297-89245316 MsG0780041293.01.T01:CDS 30.0%
GATAAGGTTTATCTTGAGAA+TGG - Chr7:89245459-89245478 MsG0780041293.01.T01:intron 30.0%
TGGTGTATGATGAAAAGAAT+TGG - Chr7:89245227-89245246 MsG0780041293.01.T01:CDS 30.0%
TTATTACTTGCATGGTTACT+GGG - Chr7:89245482-89245501 MsG0780041293.01.T01:CDS 30.0%
!!! ATACTGGTAGTTTTTTGTTG+AGG - Chr7:89245260-89245279 MsG0780041293.01.T01:CDS 30.0%
!!! GATTTTGGTTGATAGATATG+AGG - Chr7:89245506-89245525 MsG0780041293.01.T01:CDS 30.0%
AGTTCATCTTTAACCTCAAG+AGG + Chr7:89245764-89245783 None:intergenic 35.0%
CATTGGATATTCTTGATGCT+TGG - Chr7:89245296-89245315 MsG0780041293.01.T01:CDS 35.0%
CTTGAGGTTAAAGATGAACT+TGG - Chr7:89245764-89245783 MsG0780041293.01.T01:CDS 35.0%
GAGAATGGTTATTACTTGCA+TGG - Chr7:89245474-89245493 MsG0780041293.01.T01:CDS 35.0%
GGATAGTGATGCTATGTTTA+CGG - Chr7:89245135-89245154 MsG0780041293.01.T01:CDS 35.0%
GTTATTACTTGCATGGTTAC+TGG - Chr7:89245481-89245500 MsG0780041293.01.T01:CDS 35.0%
TATTACTTGCATGGTTACTG+GGG - Chr7:89245483-89245502 MsG0780041293.01.T01:CDS 35.0%
TGATGCTATGTTTACGGATA+TGG - Chr7:89245141-89245160 MsG0780041293.01.T01:CDS 35.0%
TGGATTCACTCATAAGTCAT+TGG - Chr7:89245697-89245716 MsG0780041293.01.T01:CDS 35.0%
! CTCATAAGTCATTGGTTAGT+AGG - Chr7:89245705-89245724 MsG0780041293.01.T01:CDS 35.0%
! GAGAATTATCATCCTGGTTT+TGG - Chr7:89245537-89245556 MsG0780041293.01.T01:CDS 35.0%
!! AATTGGATTGGGTTGAATAC+TGG - Chr7:89245244-89245263 MsG0780041293.01.T01:CDS 35.0%
!! ACCTCAACAGCTTTAAAAGT+CGG + Chr7:89245800-89245819 None:intergenic 35.0%
!! GATTCAAATTTGGTGATGCA+TGG - Chr7:89245193-89245212 MsG0780041293.01.T01:CDS 35.0%
AGCAGATGATCAATCTGCTA+TGG - Chr7:89245402-89245421 MsG0780041293.01.T01:intron 40.0%
ATTACTTGCATGGTTACTGG+GGG - Chr7:89245484-89245503 MsG0780041293.01.T01:CDS 40.0%
CTATGGTTTATTTGTGGCCA+AGG - Chr7:89245419-89245438 MsG0780041293.01.T01:intron 40.0%
GAATCTTTATACCTCTCCCA+CGG + Chr7:89245178-89245197 None:intergenic 40.0%
GTAGGAGAGTTAGGAGAATA+AGG - Chr7:89245723-89245742 MsG0780041293.01.T01:CDS 40.0%
TAATCACCAAACTTCCCACA+AGG + Chr7:89245605-89245624 None:intergenic 40.0%
TGAGACTAGTAATCCTCTTG+AGG - Chr7:89245748-89245767 MsG0780041293.01.T01:CDS 40.0%
TGATCATCTGCTTCGAAAAC+CGG + Chr7:89245394-89245413 None:intergenic 40.0%
TTTATTTGTGGCCAAGGAGA+AGG - Chr7:89245425-89245444 MsG0780041293.01.T01:intron 40.0%
! CATTGGTTAGTAGGAGAGTT+AGG - Chr7:89245714-89245733 MsG0780041293.01.T01:CDS 40.0%
! GAAGTTTGGTGATTATCCTG+TGG - Chr7:89245610-89245629 MsG0780041293.01.T01:CDS 40.0%
! TTTTGTTGAGGAACTGTCAG+TGG - Chr7:89245272-89245291 MsG0780041293.01.T01:CDS 40.0%
!! CAAATTTGGTGATGCATGGT+TGG - Chr7:89245197-89245216 MsG0780041293.01.T01:CDS 40.0%
!! CAACAGCTTTAAAAGTCGGA+TGG + Chr7:89245796-89245815 None:intergenic 40.0%
!! CATTGGTGACTCATTTTGTG+GGG - Chr7:89245574-89245593 MsG0780041293.01.T01:CDS 40.0%
!! CCATTGGTGACTCATTTTGT+GGG - Chr7:89245573-89245592 MsG0780041293.01.T01:CDS 40.0%
!!! TCCGACTTTTAAAGCTGTTG+AGG - Chr7:89245796-89245815 MsG0780041293.01.T01:CDS 40.0%
CCCACAAAATGAGTCACCAA+TGG + Chr7:89245576-89245595 None:intergenic 45.0%
GAAGGAAAAGTGGGGTGATA+AGG - Chr7:89245443-89245462 MsG0780041293.01.T01:intron 45.0%
GATGCATGGTTGGAATGAGA+TGG - Chr7:89245207-89245226 MsG0780041293.01.T01:CDS 45.0%
GGAAAGTTAGGGATGAAGCA+GGG - Chr7:89245338-89245357 MsG0780041293.01.T01:intron 45.0%
GTGTAACCTTGTGGGAAGTT+TGG - Chr7:89245596-89245615 MsG0780041293.01.T01:CDS 45.0%
TCATCCCTAACTTTCCCCTT+CGG + Chr7:89245334-89245353 None:intergenic 45.0%
TTACGCGCGAACTCAAAGAT+AGG - Chr7:89245368-89245387 MsG0780041293.01.T01:intron 45.0%
TTTGTGGGGTGTAACCTTGT+GGG - Chr7:89245588-89245607 MsG0780041293.01.T01:CDS 45.0%
! TTTTGTGGGGTGTAACCTTG+TGG - Chr7:89245587-89245606 MsG0780041293.01.T01:CDS 45.0%
!! GCCATTGGTGACTCATTTTG+TGG - Chr7:89245572-89245591 MsG0780041293.01.T01:CDS 45.0%
!!! ATATGGCTTTTGAAGTGCCG+TGG - Chr7:89245158-89245177 MsG0780041293.01.T01:CDS 45.0%
!!! ATCCTGGTTTTGGTGACCAT+AGG - Chr7:89245547-89245566 MsG0780041293.01.T01:CDS 45.0%
!!! TATGGCTTTTGAAGTGCCGT+GGG - Chr7:89245159-89245178 MsG0780041293.01.T01:CDS 45.0%
CACCTATGGTCACCAAAACC+AGG + Chr7:89245552-89245571 None:intergenic 50.0%
CTTGATGCTTGGGCACCAAT+GGG - Chr7:89245307-89245326 MsG0780041293.01.T01:CDS 50.0%
GAGGAACTGTCAGTGGTCAT+TGG - Chr7:89245279-89245298 MsG0780041293.01.T01:CDS 50.0%
GCCAAGGAGAAGGAAAAGTG+GGG - Chr7:89245435-89245454 MsG0780041293.01.T01:intron 50.0%
GGAGAGATGCTTGAAGCAGA+TGG - Chr7:89245631-89245650 MsG0780041293.01.T01:CDS 50.0%
GGCCAAGGAGAAGGAAAAGT+GGG - Chr7:89245434-89245453 MsG0780041293.01.T01:intron 50.0%
GGGAAAGTTAGGGATGAAGC+AGG - Chr7:89245337-89245356 MsG0780041293.01.T01:intron 50.0%
TCTTGATGCTTGGGCACCAA+TGG - Chr7:89245306-89245325 MsG0780041293.01.T01:CDS 50.0%
TGCTTCAAGCATCTCTCCAC+AGG + Chr7:89245629-89245648 None:intergenic 50.0%
TGGCCAAGGAGAAGGAAAAG+TGG - Chr7:89245433-89245452 MsG0780041293.01.T01:intron 50.0%
! GCATGGTTACTGGGGGATTT+TGG - Chr7:89245491-89245510 MsG0780041293.01.T01:CDS 50.0%
!! ACCCCACTTTTCCTTCTCCT+TGG + Chr7:89245439-89245458 None:intergenic 50.0%
!!! CTGGTTTTGGTGACCATAGG+TGG - Chr7:89245550-89245569 MsG0780041293.01.T01:CDS 50.0%
ACTTTCCCCTTCGGTCCCAT+TGG + Chr7:89245325-89245344 None:intergenic 55.0%
GAGTCACCAATGGCCACCTA+TGG + Chr7:89245566-89245585 None:intergenic 55.0%
GCGCGAACTCAAAGATAGGC+CGG - Chr7:89245372-89245391 MsG0780041293.01.T01:intron 55.0%
GGGACCGAAGGGGAAAGTTA+GGG - Chr7:89245327-89245346 MsG0780041293.01.T01:CDS 55.0%
TGGGACCGAAGGGGAAAGTT+AGG - Chr7:89245326-89245345 MsG0780041293.01.T01:CDS 55.0%
TGGTGACCATAGGTGGCCAT+TGG - Chr7:89245557-89245576 MsG0780041293.01.T01:CDS 55.0%
!!! CTTTTGAAGTGCCGTGGGAG+AGG - Chr7:89245164-89245183 MsG0780041293.01.T01:CDS 55.0%
TGGGCACCAATGGGACCGAA+GGG - Chr7:89245316-89245335 MsG0780041293.01.T01:CDS 60.0%
TTGGGCACCAATGGGACCGA+AGG - Chr7:89245315-89245334 MsG0780041293.01.T01:CDS 60.0%
GGGCACCAATGGGACCGAAG+GGG - Chr7:89245317-89245336 MsG0780041293.01.T01:CDS 65.0%
Chromosome Type Strat End Strand Name
Chr7 gene 89245133 89245835 89245133 ID=MsG0780041293.01;Name=MsG0780041293.01
Chr7 mRNA 89245133 89245835 89245133 ID=MsG0780041293.01.T01;Parent=MsG0780041293.01;Name=MsG0780041293.01.T01;_AED=0.50;_eAED=0.50;_QI=0|0|0|1|1|1|2|0|194
Chr7 exon 89245473 89245835 89245473 ID=MsG0780041293.01.T01:exon:20647;Parent=MsG0780041293.01.T01
Chr7 exon 89245133 89245354 89245133 ID=MsG0780041293.01.T01:exon:20646;Parent=MsG0780041293.01.T01
Chr7 CDS 89245473 89245835 89245473 ID=MsG0780041293.01.T01:cds;Parent=MsG0780041293.01.T01
Chr7 CDS 89245133 89245354 89245133 ID=MsG0780041293.01.T01:cds;Parent=MsG0780041293.01.T01
Gene Sequence

>MsG0780041293.01.T01

ATGGATAGTGATGCTATGTTTACGGATATGGCTTTTGAAGTGCCGTGGGAGAGGTATAAAGATTCAAATTTGGTGATGCATGGTTGGAATGAGATGGTGTATGATGAAAAGAATTGGATTGGGTTGAATACTGGTAGTTTTTTGTTGAGGAACTGTCAGTGGTCATTGGATATTCTTGATGCTTGGGCACCAATGGGACCGAAGGGGAAAGTTAGGGATGAAGCAGGGAAAATACTTACGCGCGAACTCAAAGATAGGCCGGTTTTCGAAGCAGATGATCAATCTGCTATGGTTTATTTGTGGCCAAGGAGAAGGAAAAGTGGGGTGATAAGGTTTATCTTGAGAATGGTTATTACTTGCATGTTTGGTGATTATCCTGTGGAGAGATGCTTGAAGCAGATGGATAGAGCTTTCAATTTCGGTGATAATCAAATATTGCAGATTTATGGATTCACTCATAAGTCATTGGTTAGTAGGAGAGTTAGGAGAATAAGGAATGAGACTAGTAATCCTCTTGAGGTTAAAGATGAACTTGGCTTGCTCCATCCGACTTTTAAAGCTGTTGAGGTATCATCTTCTAGCTGA

Protein sequence

>MsG0780041293.01.T01

MDSDAMFTDMAFEVPWERYKDSNLVMHGWNEMVYDEKNWIGLNTGSFLLRNCQWSLDILDAWAPMGPKGKVRDEAGKILTRELKDRPVFEADDQSAMVYLWPRRRKSGVIRFILRMVITCMFGDYPVERCLKQMDRAFNFGDNQILQIYGFTHKSLVSRRVRRIRNETSNPLEVKDELGLLHPTFKAVEVSSSS*