Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880042390.01.T01 | XP_003627006.2 | 53.179 | 173 | 37 | 4 | 15 | 144 | 23 | 194 | 8.67E-45 | 155 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880042390.01.T01 | G7LFY6 | 53.179 | 173 | 37 | 4 | 15 | 144 | 23 | 194 | 4.14e-45 | 155 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180000782.01 | MsG0880042390.01 | 0.804333 | 2.271819e-49 | 1.168182e-46 |
| MsG0880042343.01 | MsG0880042390.01 | 0.808885 | 2.485375e-50 | 1.437768e-47 |
| MsG0880042390.01 | MsG0880044538.01 | 0.804577 | 2.020876e-49 | 1.045702e-46 |
| MsG0680030680.01 | MsG0880042390.01 | 0.819309 | 1.242456e-52 | 9.526030e-50 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880042390.01.T01 | MTR_8g013990 | 53.179 | 173 | 37 | 4 | 15 | 144 | 23 | 194 | 1.05e-48 | 155 |
| MsG0880042390.01.T01 | MTR_2g101140 | 40.940 | 149 | 43 | 4 | 40 | 144 | 49 | 196 | 3.01e-21 | 85.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880042390.01.T01 | AT5G25840 | 36.691 | 139 | 48 | 2 | 46 | 144 | 35 | 173 | 2.24e-18 | 77.4 |
Find 32 sgRNAs with CRISPR-Local
Find 42 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTAACATGGATGGGAAAATT+AGG | 0.194429 | 8:-7988215 | MsG0880042390.01.T01:CDS |
| CCTCCTCCTTTCTTGTCCCT+TGG | 0.275546 | 8:+7988177 | None:intergenic |
| AAAATAGATAGATAGTTAAT+TGG | 0.284549 | 8:+7988086 | None:intergenic |
| TGAAAGCTTTGTGGTTTGTT+TGG | 0.393282 | 8:+7988590 | None:intergenic |
| GACAAGAAAGGAGGAGGAGG+AGG | 0.400333 | 8:-7988171 | MsG0880042390.01.T01:CDS |
| TAACATGGATGGGAAAATTA+GGG | 0.434099 | 8:-7988214 | MsG0880042390.01.T01:CDS |
| TTGATCTCTCTTGTAAGTGC+TGG | 0.461637 | 8:+7988123 | None:intergenic |
| ACACTTTGCATCTTCAACTT+CGG | 0.466067 | 8:+7988479 | None:intergenic |
| GTCGGAAGTTGAAAGCTTTG+TGG | 0.475347 | 8:+7988581 | None:intergenic |
| AGGGACAAGAAAGGAGGAGG+AGG | 0.494514 | 8:-7988174 | MsG0880042390.01.T01:CDS |
| TCGTGTCGTAACAAGTTTGA+AGG | 0.495357 | 8:-7988412 | MsG0880042390.01.T01:CDS |
| CCGACATTCCTTCGAGCTGA+TGG | 0.495506 | 8:-7988540 | MsG0880042390.01.T01:CDS |
| TGTTGTCAAGTTCATTCTCA+AGG | 0.519029 | 8:-7988628 | MsG0880042390.01.T01:CDS |
| TTGAAAAGAGTAACATGGAT+GGG | 0.543350 | 8:-7988224 | MsG0880042390.01.T01:CDS |
| TCGGAGACCGTTCTTTGAAG+TGG | 0.558642 | 8:+7988559 | None:intergenic |
| GTTGAAAAGAGTAACATGGA+TGG | 0.559477 | 8:-7988225 | MsG0880042390.01.T01:CDS |
| CCAAGGGACAAGAAAGGAGG+AGG | 0.566147 | 8:-7988177 | MsG0880042390.01.T01:CDS |
| GGGCTAAGTCAATTAGTCCA+AGG | 0.570684 | 8:-7988194 | MsG0880042390.01.T01:CDS |
| ATTAGTCCAAGGGACAAGAA+AGG | 0.576487 | 8:-7988183 | MsG0880042390.01.T01:CDS |
| TACGACACGATCTATGTACT+CGG | 0.583983 | 8:+7988425 | None:intergenic |
| AGACCGTTCTTTGAAGTGGT+CGG | 0.590525 | 8:+7988563 | None:intergenic |
| ACAAGAAAGGAGGAGGAGGA+GGG | 0.591395 | 8:-7988170 | MsG0880042390.01.T01:CDS |
| GGCTAAGTCAATTAGTCCAA+GGG | 0.598516 | 8:-7988193 | MsG0880042390.01.T01:CDS |
| CCATCAGCTCGAAGGAATGT+CGG | 0.601765 | 8:+7988540 | None:intergenic |
| AAATGTTGAAAAGAGTAACA+TGG | 0.604169 | 8:-7988229 | MsG0880042390.01.T01:CDS |
| CGTGTCGTAACAAGTTTGAA+GGG | 0.606556 | 8:-7988411 | MsG0880042390.01.T01:CDS |
| CTTCCGACCACTTCAAAGAA+CGG | 0.620066 | 8:-7988566 | MsG0880042390.01.T01:CDS |
| TCTTCAACTTCGGATATCGA+TGG | 0.633325 | 8:+7988489 | None:intergenic |
| ACGACACGATCTATGTACTC+GGG | 0.659077 | 8:+7988426 | None:intergenic |
| GATGCAAAGTGTGAGTGTTG+TGG | 0.665594 | 8:-7988468 | MsG0880042390.01.T01:CDS |
| AGTCCAAGGGACAAGAAAGG+AGG | 0.666513 | 8:-7988180 | MsG0880042390.01.T01:CDS |
| TTTGAAGGGAAATTTGTGTG+CGG | 0.673715 | 8:-7988397 | MsG0880042390.01.T01:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TAAGTTTAACAAATATGGTA+GGG | - | Chr8:7988447-7988466 | MsG0880042390.01.T01:CDS | 20.0% |
| !! | TGTGTTAAGTTTAACAAATA+TGG | - | Chr8:7988442-7988461 | MsG0880042390.01.T01:CDS | 20.0% |
| !! | TTAAGTTTAACAAATATGGT+AGG | - | Chr8:7988446-7988465 | MsG0880042390.01.T01:CDS | 20.0% |
| ! | AAATGTTGAAAAGAGTAACA+TGG | - | Chr8:7988505-7988524 | MsG0880042390.01.T01:CDS | 25.0% |
| TAACATGGATGGGAAAATTA+GGG | - | Chr8:7988520-7988539 | MsG0880042390.01.T01:CDS | 30.0% | |
| TTGAAAAGAGTAACATGGAT+GGG | - | Chr8:7988510-7988529 | MsG0880042390.01.T01:CDS | 30.0% | |
| ACACTTTGCATCTTCAACTT+CGG | + | Chr8:7988258-7988277 | None:intergenic | 35.0% | |
| GAAAGAAGAGTTGGAGAAAA+TGG | - | Chr8:7988378-7988397 | MsG0880042390.01.T01:intron | 35.0% | |
| GTAACATGGATGGGAAAATT+AGG | - | Chr8:7988519-7988538 | MsG0880042390.01.T01:CDS | 35.0% | |
| GTTGAAAAGAGTAACATGGA+TGG | - | Chr8:7988509-7988528 | MsG0880042390.01.T01:CDS | 35.0% | |
| TGTTGTCAAGTTCATTCTCA+AGG | - | Chr8:7988106-7988125 | MsG0880042390.01.T01:CDS | 35.0% | |
| TTTGAAGGGAAATTTGTGTG+CGG | - | Chr8:7988337-7988356 | MsG0880042390.01.T01:intron | 35.0% | |
| !! | TGAAAGCTTTGTGGTTTGTT+TGG | + | Chr8:7988147-7988166 | None:intergenic | 35.0% |
| AGAAGAGTTGGAGAAAATGG+TGG | - | Chr8:7988381-7988400 | MsG0880042390.01.T01:intron | 40.0% | |
| AGCTTGTGCTTGAAACAAAC+TGG | + | Chr8:7988478-7988497 | None:intergenic | 40.0% | |
| ATTAGTCCAAGGGACAAGAA+AGG | - | Chr8:7988551-7988570 | MsG0880042390.01.T01:CDS | 40.0% | |
| ATTTGTGTGCGGTTTGTGTT+CGG | - | Chr8:7988348-7988367 | MsG0880042390.01.T01:intron | 40.0% | |
| CGTGTCGTAACAAGTTTGAA+GGG | - | Chr8:7988323-7988342 | MsG0880042390.01.T01:intron | 40.0% | |
| GGCTAAGTCAATTAGTCCAA+GGG | - | Chr8:7988541-7988560 | MsG0880042390.01.T01:CDS | 40.0% | |
| TACGACACGATCTATGTACT+CGG | + | Chr8:7988312-7988331 | None:intergenic | 40.0% | |
| TCGTGTCGTAACAAGTTTGA+AGG | - | Chr8:7988322-7988341 | MsG0880042390.01.T01:intron | 40.0% | |
| TCTTCAACTTCGGATATCGA+TGG | + | Chr8:7988248-7988267 | None:intergenic | 40.0% | |
| TTGATCTCTCTTGTAAGTGC+TGG | + | Chr8:7988614-7988633 | None:intergenic | 40.0% | |
| ACGACACGATCTATGTACTC+GGG | + | Chr8:7988311-7988330 | None:intergenic | 45.0% | |
| CTTCCGACCACTTCAAAGAA+CGG | - | Chr8:7988168-7988187 | MsG0880042390.01.T01:CDS | 45.0% | |
| GGGCTAAGTCAATTAGTCCA+AGG | - | Chr8:7988540-7988559 | MsG0880042390.01.T01:CDS | 45.0% | |
| GTCGGAAGTTGAAAGCTTTG+TGG | + | Chr8:7988156-7988175 | None:intergenic | 45.0% | |
| ! | GATGCAAAGTGTGAGTGTTG+TGG | - | Chr8:7988266-7988285 | MsG0880042390.01.T01:intron | 45.0% |
| ! | TTCTTTTGCCATCAGCTCGA+AGG | + | Chr8:7988205-7988224 | None:intergenic | 45.0% |
| !! | AGACCGTTCTTTGAAGTGGT+CGG | + | Chr8:7988174-7988193 | None:intergenic | 45.0% |
| ACAAGAAAGGAGGAGGAGGA+GGG | - | Chr8:7988564-7988583 | MsG0880042390.01.T01:CDS | 50.0% | |
| AGTCCAAGGGACAAGAAAGG+AGG | - | Chr8:7988554-7988573 | MsG0880042390.01.T01:CDS | 50.0% | |
| CCATCAGCTCGAAGGAATGT+CGG | + | Chr8:7988197-7988216 | None:intergenic | 50.0% | |
| ! | TCGGAGACCGTTCTTTGAAG+TGG | + | Chr8:7988178-7988197 | None:intergenic | 50.0% |
| AGGGACAAGAAAGGAGGAGG+AGG | - | Chr8:7988560-7988579 | MsG0880042390.01.T01:CDS | 55.0% | |
| CCAAGGGACAAGAAAGGAGG+AGG | - | Chr8:7988557-7988576 | MsG0880042390.01.T01:CDS | 55.0% | |
| CCGACATTCCTTCGAGCTGA+TGG | - | Chr8:7988194-7988213 | MsG0880042390.01.T01:CDS | 55.0% | |
| CCTCCTCCTTTCTTGTCCCT+TGG | + | Chr8:7988560-7988579 | None:intergenic | 55.0% | |
| GACAAGAAAGGAGGAGGAGG+AGG | - | Chr8:7988563-7988582 | MsG0880042390.01.T01:CDS | 55.0% | |
| GGAGGCGGTGAAAGAAGAGT+TGG | - | Chr8:7988369-7988388 | MsG0880042390.01.T01:intron | 55.0% | |
| TGTGTGCGGTTTGTGTTCGG+AGG | - | Chr8:7988351-7988370 | MsG0880042390.01.T01:intron | 55.0% | |
| GTGCGGTTTGTGTTCGGAGG+CGG | - | Chr8:7988354-7988373 | MsG0880042390.01.T01:intron | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr8 | gene | 7988101 | 7988655 | 7988101 | ID=MsG0880042390.01;Name=MsG0880042390.01 |
| Chr8 | mRNA | 7988101 | 7988655 | 7988101 | ID=MsG0880042390.01.T01;Parent=MsG0880042390.01;Name=MsG0880042390.01.T01;_AED=0.40;_eAED=0.43;_QI=0|0|0|1|1|1|2|0|144 |
| Chr8 | exon | 7988398 | 7988655 | 7988398 | ID=MsG0880042390.01.T01:exon:35712;Parent=MsG0880042390.01.T01 |
| Chr8 | exon | 7988101 | 7988277 | 7988101 | ID=MsG0880042390.01.T01:exon:35713;Parent=MsG0880042390.01.T01 |
| Chr8 | CDS | 7988398 | 7988655 | 7988398 | ID=MsG0880042390.01.T01:cds;Parent=MsG0880042390.01.T01 |
| Chr8 | CDS | 7988101 | 7988277 | 7988101 | ID=MsG0880042390.01.T01:cds;Parent=MsG0880042390.01.T01 |
| Gene Sequence |
| Protein sequence |
