Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880042522.01.T01 | RHN39228.1 | 88.75 | 80 | 9 | 0 | 13 | 92 | 1 | 80 | 2.30E-42 | 144 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880042522.01.T01 | I1KUX4 | 61.832 | 131 | 28 | 2 | 13 | 121 | 1 | 131 | 2.98e-40 | 151 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048787.01 | MsG0880042522.01 | 0.803038 | 4.219075e-49 | 2.098473e-46 |
| MsG0180000091.01 | MsG0880042522.01 | 0.804591 | 2.007327e-49 | 1.039062e-46 |
| MsG0380013363.01 | MsG0880042522.01 | 0.819409 | 1.179418e-52 | 9.068440e-50 |
| MsG0480021501.01 | MsG0880042522.01 | 0.806926 | 6.489363e-50 | 3.566639e-47 |
| MsG0480023354.01 | MsG0880042522.01 | 0.808209 | 3.466098e-50 | 1.970027e-47 |
| MsG0280008765.01 | MsG0880042522.01 | 0.803999 | 2.665496e-49 | 1.358990e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880042522.01.T01 | MTR_8g015600 | 66.412 | 131 | 22 | 2 | 13 | 121 | 1 | 131 | 2.62e-41 | 144 |
| MsG0880042522.01.T01 | MTR_8g015600 | 66.412 | 131 | 22 | 2 | 13 | 121 | 1 | 131 | 2.65e-41 | 144 |
| MsG0880042522.01.T01 | MTR_8g015610 | 85.333 | 75 | 11 | 0 | 13 | 87 | 1 | 75 | 2.13e-36 | 131 |
| MsG0880042522.01.T01 | MTR_1g021885 | 41.284 | 109 | 58 | 3 | 13 | 121 | 1 | 103 | 9.12e-16 | 70.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880042522.01.T01 | AT1G51720 | 51.908 | 131 | 41 | 2 | 13 | 121 | 1 | 131 | 2.41e-28 | 107 |
| MsG0880042522.01.T01 | AT1G51720 | 51.908 | 131 | 41 | 2 | 13 | 121 | 1 | 131 | 3.07e-28 | 108 |
| MsG0880042522.01.T01 | AT1G51720 | 51.908 | 131 | 41 | 2 | 13 | 121 | 1 | 131 | 3.07e-28 | 108 |
| MsG0880042522.01.T01 | AT1G51720 | 51.908 | 131 | 41 | 2 | 13 | 121 | 1 | 131 | 3.19e-28 | 107 |
Find 26 sgRNAs with CRISPR-Local
Find 30 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AATGGGCAGATACTTATAAA+TGG | 0.255985 | 8:-9957452 | MsG0880042522.01.T01:CDS |
| ACAGGTGAAAAGGAAGAAAA+TGG | 0.344196 | 8:-9957496 | MsG0880042522.01.T01:CDS |
| GACGACATTGTCTTAGATAA+TGG | 0.357850 | 8:-9957600 | MsG0880042522.01.T01:CDS |
| GGAGAGGACGATCCTTCATT+TGG | 0.406683 | 8:-9957573 | MsG0880042522.01.T01:CDS |
| CTCATACTCAACAGGTGAAA+AGG | 0.436078 | 8:-9957506 | MsG0880042522.01.T01:CDS |
| AGATTTGTCTCATACTCAAC+AGG | 0.452612 | 8:-9957514 | MsG0880042522.01.T01:CDS |
| CAATGGTTGGCAGCAACATT+CGG | 0.462412 | 8:+9957706 | None:intergenic |
| ACAATTTCTTCACCAAATGA+AGG | 0.476033 | 8:+9957561 | None:intergenic |
| CGAATGTTGCTGCCAACCAT+TGG | 0.479378 | 8:-9957705 | MsG0880042522.01.T01:CDS |
| CACTTGGTTGTGAACGAGAT+TGG | 0.492671 | 8:-9957624 | MsG0880042522.01.T01:CDS |
| ATGGGCAGATACTTATAAAT+GGG | 0.495944 | 8:-9957451 | MsG0880042522.01.T01:CDS |
| TGCAGACAGCAGAAAATCCT+TGG | 0.504867 | 8:+9957402 | None:intergenic |
| AATGAAGGATCGTCCTCTCC+AGG | 0.510240 | 8:+9957576 | None:intergenic |
| TAAAAGACGGAGAGAGGAAT+GGG | 0.521660 | 8:-9957469 | MsG0880042522.01.T01:CDS |
| TTGCTGCCAACCATTGGAGT+AGG | 0.526190 | 8:-9957699 | MsG0880042522.01.T01:CDS |
| ATCATCCCTACTCCAATGGT+TGG | 0.560312 | 8:+9957693 | None:intergenic |
| GGCTGTTAAAAGACGGAGAG+AGG | 0.562084 | 8:-9957475 | MsG0880042522.01.T01:CDS |
| GCAAGCACATAGGCATCACT+TGG | 0.572850 | 8:-9957640 | MsG0880042522.01.T01:CDS |
| TTAAAAGACGGAGAGAGGAA+TGG | 0.579738 | 8:-9957470 | MsG0880042522.01.T01:CDS |
| AGTAGGGATGATTTCTGCAA+TGG | 0.590120 | 8:-9957682 | MsG0880042522.01.T01:CDS |
| ATTGTCTTAGATAATGGACC+TGG | 0.607953 | 8:-9957594 | MsG0880042522.01.T01:CDS |
| AGAAAATGGCTGTTAAAAGA+CGG | 0.615152 | 8:-9957482 | MsG0880042522.01.T01:CDS |
| ATTTGATTCAGCAAGCACAT+AGG | 0.615701 | 8:-9957650 | MsG0880042522.01.T01:CDS |
| AGAAATCATCCCTACTCCAA+TGG | 0.619665 | 8:+9957689 | None:intergenic |
| TGCTGCCAACCATTGGAGTA+GGG | 0.624282 | 8:-9957698 | MsG0880042522.01.T01:CDS |
| CTTAGATAATGGACCTGGAG+AGG | 0.632290 | 8:-9957589 | MsG0880042522.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AATGGGCAGATACTTATAAA+TGG | - | Chr8:9957678-9957697 | MsG0880042522.01.T01:CDS | 30.0% | |
| ACAATTTCTTCACCAAATGA+AGG | + | Chr8:9957572-9957591 | None:intergenic | 30.0% | |
| AGAAAATGGCTGTTAAAAGA+CGG | - | Chr8:9957648-9957667 | MsG0880042522.01.T01:CDS | 30.0% | |
| ATGGGCAGATACTTATAAAT+GGG | - | Chr8:9957679-9957698 | MsG0880042522.01.T01:CDS | 30.0% | |
| CTTATGTCGATGTGAAAAAA+AGG | - | Chr8:9957702-9957721 | MsG0880042522.01.T01:CDS | 30.0% | |
| ACAGGTGAAAAGGAAGAAAA+TGG | - | Chr8:9957634-9957653 | MsG0880042522.01.T01:CDS | 35.0% | |
| AGATTTGTCTCATACTCAAC+AGG | - | Chr8:9957616-9957635 | MsG0880042522.01.T01:CDS | 35.0% | |
| ATGTGAAAAAAAGGACACCA+AGG | - | Chr8:9957711-9957730 | MsG0880042522.01.T01:CDS | 35.0% | |
| ATTTGATTCAGCAAGCACAT+AGG | - | Chr8:9957480-9957499 | MsG0880042522.01.T01:CDS | 35.0% | |
| GACGACATTGTCTTAGATAA+TGG | - | Chr8:9957530-9957549 | MsG0880042522.01.T01:CDS | 35.0% | |
| ! | ATGCTTTTAGATTTGTGGAG+AGG | - | Chr8:9957392-9957411 | MsG0880042522.01.T01:CDS | 35.0% |
| ! | TGCTTTTAGATTTGTGGAGA+GGG | - | Chr8:9957393-9957412 | MsG0880042522.01.T01:CDS | 35.0% |
| !! | ATTGTCTTAGATAATGGACC+TGG | - | Chr8:9957536-9957555 | MsG0880042522.01.T01:CDS | 35.0% |
| AGAAATCATCCCTACTCCAA+TGG | + | Chr8:9957444-9957463 | None:intergenic | 40.0% | |
| AGTAGGGATGATTTCTGCAA+TGG | - | Chr8:9957448-9957467 | MsG0880042522.01.T01:CDS | 40.0% | |
| CTCATACTCAACAGGTGAAA+AGG | - | Chr8:9957624-9957643 | MsG0880042522.01.T01:CDS | 40.0% | |
| TAAAAGACGGAGAGAGGAAT+GGG | - | Chr8:9957661-9957680 | MsG0880042522.01.T01:CDS | 40.0% | |
| TTAAAAGACGGAGAGAGGAA+TGG | - | Chr8:9957660-9957679 | MsG0880042522.01.T01:CDS | 40.0% | |
| ATCATCCCTACTCCAATGGT+TGG | + | Chr8:9957440-9957459 | None:intergenic | 45.0% | |
| CAATGGTTGGCAGCAACATT+CGG | + | Chr8:9957427-9957446 | None:intergenic | 45.0% | |
| CACTTGGTTGTGAACGAGAT+TGG | - | Chr8:9957506-9957525 | MsG0880042522.01.T01:CDS | 45.0% | |
| TGCAGACAGCAGAAAATCCT+TGG | + | Chr8:9957731-9957750 | None:intergenic | 45.0% | |
| !! | CTTAGATAATGGACCTGGAG+AGG | - | Chr8:9957541-9957560 | MsG0880042522.01.T01:CDS | 45.0% |
| AATGAAGGATCGTCCTCTCC+AGG | + | Chr8:9957557-9957576 | None:intergenic | 50.0% | |
| CGAATGTTGCTGCCAACCAT+TGG | - | Chr8:9957425-9957444 | MsG0880042522.01.T01:CDS | 50.0% | |
| GCAAGCACATAGGCATCACT+TGG | - | Chr8:9957490-9957509 | MsG0880042522.01.T01:CDS | 50.0% | |
| GGAGAGGACGATCCTTCATT+TGG | - | Chr8:9957557-9957576 | MsG0880042522.01.T01:CDS | 50.0% | |
| GGCTGTTAAAAGACGGAGAG+AGG | - | Chr8:9957655-9957674 | MsG0880042522.01.T01:CDS | 50.0% | |
| TGCTGCCAACCATTGGAGTA+GGG | - | Chr8:9957432-9957451 | MsG0880042522.01.T01:CDS | 50.0% | |
| TTGCTGCCAACCATTGGAGT+AGG | - | Chr8:9957431-9957450 | MsG0880042522.01.T01:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr8 | gene | 9957392 | 9957760 | 9957392 | ID=MsG0880042522.01;Name=MsG0880042522.01 |
| Chr8 | mRNA | 9957392 | 9957760 | 9957392 | ID=MsG0880042522.01.T01;Parent=MsG0880042522.01;Name=MsG0880042522.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|122 |
| Chr8 | exon | 9957392 | 9957760 | 9957392 | ID=MsG0880042522.01.T01:exon:3019;Parent=MsG0880042522.01.T01 |
| Chr8 | CDS | 9957392 | 9957760 | 9957392 | ID=MsG0880042522.01.T01:cds;Parent=MsG0880042522.01.T01 |
| Gene Sequence |
| Protein sequence |
