Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880042586.01.T01 | PNX75762.1 | 70.312 | 128 | 34 | 2 | 1 | 128 | 1 | 124 | 1.29E-23 | 100 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880042586.01.T01 | A0A2K3LB45 | 70.312 | 128 | 34 | 2 | 1 | 128 | 1 | 124 | 6.15e-24 | 100 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0380014135.01 | MsG0880042586.01 | 0.806162 | 9.405101e-50 | 5.069175e-47 |
| MsG0480022067.01 | MsG0880042586.01 | 0.825183 | 5.386323e-54 | 4.874460e-51 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880042586.01.T01 | MTR_8g017180 | 78.788 | 132 | 24 | 2 | 1 | 128 | 1 | 132 | 6.06e-24 | 91.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 27 sgRNAs with CRISPR-Local
Find 34 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGAATTTGGGGATTGGAATT+AGG | 0.122258 | 8:+11008572 | None:intergenic |
| GAATTTGGGGATTGGAATTA+GGG | 0.195514 | 8:+11008573 | None:intergenic |
| ATTGAGATTGAGATTGAATT+TGG | 0.228742 | 8:+11008558 | None:intergenic |
| TTGAGATTGAGATTGAATTT+GGG | 0.338202 | 8:+11008559 | None:intergenic |
| AATGCATCTGATTGCTGATC+CGG | 0.391333 | 8:+11008165 | None:intergenic |
| TGTTGTTGAAGATAATGATT+TGG | 0.397936 | 8:+11008524 | None:intergenic |
| GATAAAGTTGTTGTTGTTGT+TGG | 0.407429 | 8:+11008435 | None:intergenic |
| GTTGTTGAAGATAATGATTT+GGG | 0.427978 | 8:+11008525 | None:intergenic |
| TTGAGATTGAATTTGGGGAT+TGG | 0.432517 | 8:+11008565 | None:intergenic |
| GGGGATTGGAATTAGGGGTT+GGG | 0.433474 | 8:+11008579 | None:intergenic |
| GTTGTTTGAGGTTGTTGAAA+AGG | 0.447188 | 8:+11008470 | None:intergenic |
| TGGGGATTGGAATTAGGGGT+TGG | 0.459404 | 8:+11008578 | None:intergenic |
| AATTGAGATTGTGTTGTTTG+AGG | 0.469049 | 8:+11008458 | None:intergenic |
| ATGCATCTGATTGCTGATCC+GGG | 0.505166 | 8:+11008166 | None:intergenic |
| GTTTCCATGTTCAACAACTT+CGG | 0.530986 | 8:+11008188 | None:intergenic |
| AGATTCTGTTGTTGCTGTGG+AGG | 0.533373 | 8:+11008362 | None:intergenic |
| ATGGAGCATTACTCTTCTCC+TGG | 0.537394 | 8:-11008620 | MsG0880042586.01.T01:CDS |
| TTAGCCGAAGTTGTTGAACA+TGG | 0.546188 | 8:-11008192 | MsG0880042586.01.T01:CDS |
| TGAAGATTCTGTTGTTGCTG+TGG | 0.552311 | 8:+11008359 | None:intergenic |
| ACTCTTCTCCTGGTGGAACA+TGG | 0.560525 | 8:-11008610 | MsG0880042586.01.T01:CDS |
| GGATCAGCAATCAGATGCAT+TGG | 0.561731 | 8:-11008163 | MsG0880042586.01.T01:CDS |
| ATCATCGTCCATGTTCCACC+AGG | 0.591081 | 8:+11008602 | None:intergenic |
| TGAGATTGAGATTGAATTTG+GGG | 0.613481 | 8:+11008560 | None:intergenic |
| AGTTGTTGAACATGGAAACC+CGG | 0.631175 | 8:-11008184 | MsG0880042586.01.T01:CDS |
| AATTTGGGGATTGGAATTAG+GGG | 0.642153 | 8:+11008574 | None:intergenic |
| GAGCATTACTCTTCTCCTGG+TGG | 0.662821 | 8:-11008617 | MsG0880042586.01.T01:CDS |
| AAGGTGTAAAGATAGAACAA+TGG | 0.679707 | 8:-11008639 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAATAATAGAAACGAAAATT+AGG | + | Chr8:11008511-11008530 | None:intergenic | 15.0% |
| !! | AATAATAGAAACGAAAATTA+GGG | + | Chr8:11008510-11008529 | None:intergenic | 15.0% |
| ! | ATTGAGATTGAGATTGAATT+TGG | + | Chr8:11008229-11008248 | None:intergenic | 25.0% |
| ! | GTTGTTGAAGATAATGATTT+GGG | + | Chr8:11008262-11008281 | None:intergenic | 25.0% |
| ! | TAATTGAAGATTTCTTCTTG+TGG | - | Chr8:11008551-11008570 | MsG0880042586.01.T01:CDS | 25.0% |
| ! | TGTTGTTGAAGATAATGATT+TGG | + | Chr8:11008263-11008282 | None:intergenic | 25.0% |
| ! | TTGAGATTGAGATTGAATTT+GGG | + | Chr8:11008228-11008247 | None:intergenic | 25.0% |
| TGAGATTGAGATTGAATTTG+GGG | + | Chr8:11008227-11008246 | None:intergenic | 30.0% | |
| TTTCTTCTTGTGGATATAGT+TGG | - | Chr8:11008561-11008580 | MsG0880042586.01.T01:CDS | 30.0% | |
| ! | ACGAAAATTAGGGTTTTTGA+AGG | + | Chr8:11008500-11008519 | None:intergenic | 30.0% |
| ! | CGAAAATTAGGGTTTTTGAA+GGG | + | Chr8:11008499-11008518 | None:intergenic | 30.0% |
| ! | GATAAAGTTGTTGTTGTTGT+TGG | + | Chr8:11008352-11008371 | None:intergenic | 30.0% |
| !! | AATTGAGATTGTGTTGTTTG+AGG | + | Chr8:11008329-11008348 | None:intergenic | 30.0% |
| !! | GAAAATTAGGGTTTTTGAAG+GGG | + | Chr8:11008498-11008517 | None:intergenic | 30.0% |
| AATTTGGGGATTGGAATTAG+GGG | + | Chr8:11008213-11008232 | None:intergenic | 35.0% | |
| GAATTTGGGGATTGGAATTA+GGG | + | Chr8:11008214-11008233 | None:intergenic | 35.0% | |
| GTTTCCATGTTCAACAACTT+CGG | + | Chr8:11008599-11008618 | None:intergenic | 35.0% | |
| TGAATTTGGGGATTGGAATT+AGG | + | Chr8:11008215-11008234 | None:intergenic | 35.0% | |
| TTGAGATTGAATTTGGGGAT+TGG | + | Chr8:11008222-11008241 | None:intergenic | 35.0% | |
| ! | GTTGTTTGAGGTTGTTGAAA+AGG | + | Chr8:11008317-11008336 | None:intergenic | 35.0% |
| AATGCATCTGATTGCTGATC+CGG | + | Chr8:11008622-11008641 | None:intergenic | 40.0% | |
| AGTTGTTGAACATGGAAACC+CGG | - | Chr8:11008600-11008619 | MsG0880042586.01.T01:CDS | 40.0% | |
| CTTCTTGTGGATATAGTTGG+TGG | - | Chr8:11008564-11008583 | MsG0880042586.01.T01:CDS | 40.0% | |
| TGAAGATTCTGTTGTTGCTG+TGG | + | Chr8:11008428-11008447 | None:intergenic | 40.0% | |
| TTAGCCGAAGTTGTTGAACA+TGG | - | Chr8:11008592-11008611 | MsG0880042586.01.T01:CDS | 40.0% | |
| AGATTCTGTTGTTGCTGTGG+AGG | + | Chr8:11008425-11008444 | None:intergenic | 45.0% | |
| ATGCATCTGATTGCTGATCC+GGG | + | Chr8:11008621-11008640 | None:intergenic | 45.0% | |
| GGATCAGCAATCAGATGCAT+TGG | - | Chr8:11008621-11008640 | MsG0880042586.01.T01:CDS | 45.0% | |
| ! | ATGGAGCATTACTCTTCTCC+TGG | - | Chr8:11008164-11008183 | MsG0880042586.01.T01:CDS | 45.0% |
| ACTCTTCTCCTGGTGGAACA+TGG | - | Chr8:11008174-11008193 | MsG0880042586.01.T01:CDS | 50.0% | |
| ATCATCGTCCATGTTCCACC+AGG | + | Chr8:11008185-11008204 | None:intergenic | 50.0% | |
| GAGCATTACTCTTCTCCTGG+TGG | - | Chr8:11008167-11008186 | MsG0880042586.01.T01:CDS | 50.0% | |
| GGGGATTGGAATTAGGGGTT+GGG | + | Chr8:11008208-11008227 | None:intergenic | 50.0% | |
| TGGGGATTGGAATTAGGGGT+TGG | + | Chr8:11008209-11008228 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr8 | gene | 11008158 | 11008648 | 11008158 | ID=MsG0880042586.01;Name=MsG0880042586.01 |
| Chr8 | mRNA | 11008158 | 11008648 | 11008158 | ID=MsG0880042586.01.T01;Parent=MsG0880042586.01;Name=MsG0880042586.01.T01;_AED=0.44;_eAED=0.44;_QI=6|1|0.5|1|0|0|2|0|128 |
| Chr8 | exon | 11008158 | 11008226 | 11008158 | ID=MsG0880042586.01.T01:exon:4539;Parent=MsG0880042586.01.T01 |
| Chr8 | exon | 11008325 | 11008648 | 11008325 | ID=MsG0880042586.01.T01:exon:4538;Parent=MsG0880042586.01.T01 |
| Chr8 | five_prime_UTR | 11008643 | 11008648 | 11008643 | ID=MsG0880042586.01.T01:five_prime_utr;Parent=MsG0880042586.01.T01 |
| Chr8 | CDS | 11008325 | 11008642 | 11008325 | ID=MsG0880042586.01.T01:cds;Parent=MsG0880042586.01.T01 |
| Chr8 | CDS | 11008158 | 11008226 | 11008158 | ID=MsG0880042586.01.T01:cds;Parent=MsG0880042586.01.T01 |
| Gene Sequence |
| Protein sequence |
