AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0380014135.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380014135.01.T01 MTR_7g026095 34.862 109 67 3 12 120 27 131 4.93e-12 61.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380014135.01.T01 AT4G23160 35.345 116 67 4 6 119 373 482 1.33e-12 63.5
MsG0380014135.01.T01 AT4G23160 35.345 116 67 4 6 119 373 482 1.33e-12 63.5

Find 21 sgRNAs with CRISPR-Local

Find 22 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCTGATTATTGTATGTTCT+TGG 0.253487 3:-50246169 MsG0380014135.01.T01:CDS
TTAAATAGTAGTCCTCTATC+AGG 0.319322 3:+50246276 None:intergenic
CTCTCAAGTCATACATGATT+TGG 0.334299 3:+50246349 None:intergenic
TCTGATTATTGTATGTTCTT+GGG 0.371727 3:-50246168 MsG0380014135.01.T01:CDS
TTGAAGTCTACACCTGATAG+AGG 0.454279 3:-50246288 MsG0380014135.01.T01:CDS
CCTGCCGAAGCTTATGCAAT+TGG 0.459634 3:-50246381 MsG0380014135.01.T01:CDS
AGTAAGAAACATAACGTAGT+TGG 0.467100 3:-50246123 MsG0380014135.01.T01:CDS
AGATTATGAACAAATTCCTT+CGG 0.468352 3:-50246466 None:intergenic
TATACCAATTGCATAAGCTT+CGG 0.517920 3:+50246377 None:intergenic
TGGGTGGAAACTTAGTTGCA+TGG 0.528075 3:-50246149 MsG0380014135.01.T01:CDS
ACGGAAGTTTATACATATGT+TGG 0.537384 3:-50246224 MsG0380014135.01.T01:CDS
GATTATTGTATGTTCTTGGG+TGG 0.539697 3:-50246165 MsG0380014135.01.T01:CDS
CAAAGCTCAATATCAAAGGT+TGG 0.556860 3:-50246439 MsG0380014135.01.T01:CDS
GCTCAATATCAAAGGTTGGT+GGG 0.562205 3:-50246435 MsG0380014135.01.T01:CDS
CCAATTGCATAAGCTTCGGC+AGG 0.562617 3:+50246381 None:intergenic
TATTGAGCTTTGTCAACCGA+AGG 0.566720 3:+50246450 None:intergenic
TTGACAAAGCTCAATATCAA+AGG 0.574128 3:-50246443 MsG0380014135.01.T01:CDS
AGCTCAATATCAAAGGTTGG+TGG 0.575704 3:-50246436 MsG0380014135.01.T01:CDS
TATACAAGATCAGTCACAGA+TGG 0.580497 3:-50246200 MsG0380014135.01.T01:CDS
ATACAAGATCAGTCACAGAT+GGG 0.628709 3:-50246199 MsG0380014135.01.T01:CDS
GTGGAAACTTAGTTGCATGG+AGG 0.725566 3:-50246146 MsG0380014135.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTAAAAAAAAAAAAAAAAAA+AGG - Chr3:50246300-50246319 MsG0380014135.01.T01:CDS 0.0%
! AAAAAAAGGAGATTCATGTA+CGG - Chr3:50246314-50246333 MsG0380014135.01.T01:CDS 25.0%
! TCTGATTATTGTATGTTCTT+GGG - Chr3:50246389-50246408 MsG0380014135.01.T01:CDS 25.0%
! TTCTGATTATTGTATGTTCT+TGG - Chr3:50246388-50246407 MsG0380014135.01.T01:CDS 25.0%
ACGGAAGTTTATACATATGT+TGG - Chr3:50246333-50246352 MsG0380014135.01.T01:CDS 30.0%
AGTAAGAAACATAACGTAGT+TGG - Chr3:50246434-50246453 MsG0380014135.01.T01:CDS 30.0%
TATACCAATTGCATAAGCTT+CGG + Chr3:50246183-50246202 None:intergenic 30.0%
TTAAATAGTAGTCCTCTATC+AGG + Chr3:50246284-50246303 None:intergenic 30.0%
TTGACAAAGCTCAATATCAA+AGG - Chr3:50246114-50246133 MsG0380014135.01.T01:CDS 30.0%
ATACAAGATCAGTCACAGAT+GGG - Chr3:50246358-50246377 MsG0380014135.01.T01:CDS 35.0%
CAAAGCTCAATATCAAAGGT+TGG - Chr3:50246118-50246137 MsG0380014135.01.T01:CDS 35.0%
CTCTCAAGTCATACATGATT+TGG + Chr3:50246211-50246230 None:intergenic 35.0%
GATTATTGTATGTTCTTGGG+TGG - Chr3:50246392-50246411 MsG0380014135.01.T01:CDS 35.0%
TATACAAGATCAGTCACAGA+TGG - Chr3:50246357-50246376 MsG0380014135.01.T01:CDS 35.0%
AGCTCAATATCAAAGGTTGG+TGG - Chr3:50246121-50246140 MsG0380014135.01.T01:CDS 40.0%
TTGAAGTCTACACCTGATAG+AGG - Chr3:50246269-50246288 MsG0380014135.01.T01:CDS 40.0%
! GCTCAATATCAAAGGTTGGT+GGG - Chr3:50246122-50246141 MsG0380014135.01.T01:CDS 40.0%
! TATTGAGCTTTGTCAACCGA+AGG + Chr3:50246110-50246129 None:intergenic 40.0%
GTGGAAACTTAGTTGCATGG+AGG - Chr3:50246411-50246430 MsG0380014135.01.T01:CDS 45.0%
TGGGTGGAAACTTAGTTGCA+TGG - Chr3:50246408-50246427 MsG0380014135.01.T01:CDS 45.0%
CCAATTGCATAAGCTTCGGC+AGG + Chr3:50246179-50246198 None:intergenic 50.0%
CCTGCCGAAGCTTATGCAAT+TGG - Chr3:50246176-50246195 MsG0380014135.01.T01:CDS 50.0%
Chromosome Type Strat End Strand Name
Chr3 gene 50246098 50246481 50246098 ID=MsG0380014135.01;Name=MsG0380014135.01
Chr3 mRNA 50246098 50246481 50246098 ID=MsG0380014135.01.T01;Parent=MsG0380014135.01;Name=MsG0380014135.01.T01;_AED=0.22;_eAED=0.22;_QI=0|-1|0|1|-1|0|1|0|127
Chr3 exon 50246098 50246481 50246098 ID=MsG0380014135.01.T01:exon:9646;Parent=MsG0380014135.01.T01
Chr3 CDS 50246098 50246481 50246098 ID=MsG0380014135.01.T01:cds;Parent=MsG0380014135.01.T01
Gene Sequence

>MsG0380014135.01.T01

GAACAAATTCCTTCGGTTGACAAAGCTCAATATCAAAGGTTGGTGGGAAAATCTATATATTGTGCTCATCATACTAGACCTGCCGAAGCTTATGCAATTGGTATAGTTAGCCAAATCATGTATGACTTGAGAGTGAGACATTTACAAGCACAATGTAGACTTTTACAAATTTTGAAGTCTACACCTGATAGAGGACTACTATTTAAAAAAAAAAAAAAAAAAAGGAGATTCATGTACGGAAGTTTATACATATGTTGGCTATACAAGATCAGTCACAGATGGGAATCAACTTCTGATTATTGTATGTTCTTGGGTGGAAACTTAGTTGCATGGAGGAGTAAGAAACATAACGTAGTTGGTTTGCAAGTGAAGCAGAAAAGTTGA

Protein sequence

>MsG0380014135.01.T01

EQIPSVDKAQYQRLVGKSIYCAHHTRPAEAYAIGIVSQIMYDLRVRHLQAQCRLLQILKSTPDRGLLFKKKKKKRRFMYGSLYICWLYKISHRWESTSDYCMFLGGNLVAWRSKKHNVVGLQVKQKS*