Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880042738.01.T01 | XP_003627291.2 | 81.013 | 316 | 57 | 1 | 89 | 404 | 855 | 1167 | 4.00E-155 | 478 |
| MsG0880042738.01.T01 | XP_003627291.2 | 60.92 | 87 | 34 | 0 | 1 | 87 | 286 | 372 | 2.71E-21 | 108 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880042738.01.T01 | Q9XIF0 | 29.373 | 303 | 187 | 9 | 91 | 389 | 589 | 868 | 7.67E-21 | 98.6 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880042738.01.T01 | A0A396GFJ2 | 81.013 | 316 | 57 | 1 | 89 | 404 | 855 | 1167 | 1.91e-155 | 478 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180002935.01 | MsG0880042738.01 | 0.800916 | 1.151575e-48 | 5.427664e-46 |
| MsG0380011635.01 | MsG0880042738.01 | 0.814660 | 1.376182e-51 | 9.289182e-49 |
| MsG0480022833.01 | MsG0880042738.01 | 0.803001 | 4.293227e-49 | 2.133305e-46 |
| MsG0280010410.01 | MsG0880042738.01 | 0.815081 | 1.109554e-51 | 7.575200e-49 |
| MsG0780036960.01 | MsG0880042738.01 | 0.808476 | 3.039391e-50 | 1.739650e-47 |
| MsG0780038519.01 | MsG0880042738.01 | 0.811013 | 8.655944e-51 | 5.298932e-48 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880042738.01.T01 | MTR_8g020790 | 80.696 | 316 | 58 | 1 | 89 | 404 | 855 | 1167 | 1.67e-158 | 476 |
| MsG0880042738.01.T01 | MTR_8g020790 | 60.920 | 87 | 34 | 0 | 1 | 87 | 286 | 372 | 3.05e-25 | 108 |
| MsG0880042738.01.T01 | MTR_1g022070 | 78.333 | 180 | 39 | 0 | 89 | 268 | 509 | 688 | 1.32e-70 | 235 |
| MsG0880042738.01.T01 | MTR_1g090690 | 43.960 | 298 | 161 | 4 | 89 | 381 | 1236 | 1532 | 7.21e-62 | 216 |
| MsG0880042738.01.T01 | MTR_1g090673 | 41.139 | 316 | 158 | 5 | 89 | 381 | 1060 | 1370 | 1.95e-56 | 200 |
| MsG0880042738.01.T01 | MTR_1g090680 | 41.346 | 312 | 163 | 4 | 89 | 381 | 1155 | 1465 | 2.58e-56 | 200 |
| MsG0880042738.01.T01 | MTR_2g038510 | 32.886 | 298 | 182 | 10 | 91 | 385 | 619 | 901 | 2.79e-22 | 99.8 |
| MsG0880042738.01.T01 | MTR_7g015770 | 31.949 | 313 | 177 | 10 | 92 | 385 | 619 | 914 | 5.98e-21 | 95.9 |
| MsG0880042738.01.T01 | MTR_3g056585 | 30.818 | 318 | 177 | 9 | 92 | 389 | 592 | 886 | 1.13e-19 | 92.0 |
| MsG0880042738.01.T01 | MTR_5g027900 | 30.592 | 304 | 177 | 9 | 102 | 386 | 596 | 884 | 8.10e-19 | 89.4 |
| MsG0880042738.01.T01 | MTR_3g011390 | 28.344 | 314 | 200 | 8 | 91 | 389 | 603 | 906 | 1.13e-18 | 89.0 |
| MsG0880042738.01.T01 | MTR_5g027860 | 29.938 | 324 | 195 | 9 | 82 | 386 | 572 | 882 | 1.85e-18 | 88.2 |
| MsG0880042738.01.T01 | MTR_1g016210 | 31.596 | 307 | 185 | 8 | 92 | 385 | 606 | 900 | 2.63e-18 | 87.8 |
| MsG0880042738.01.T01 | MTR_3g011360 | 27.044 | 318 | 199 | 8 | 91 | 389 | 569 | 872 | 1.14e-17 | 85.9 |
| MsG0880042738.01.T01 | MTR_5g021080 | 30.769 | 299 | 193 | 9 | 92 | 385 | 598 | 887 | 1.32e-17 | 85.5 |
| MsG0880042738.01.T01 | MTR_5g027870 | 31.611 | 329 | 168 | 16 | 85 | 381 | 577 | 880 | 1.92e-17 | 85.1 |
| MsG0880042738.01.T01 | MTR_3g055930 | 31.597 | 288 | 180 | 8 | 92 | 370 | 617 | 896 | 2.83e-17 | 84.7 |
| MsG0880042738.01.T01 | MTR_3g012240 | 29.808 | 312 | 180 | 9 | 92 | 381 | 647 | 941 | 3.68e-17 | 84.3 |
| MsG0880042738.01.T01 | MTR_3g069210 | 30.272 | 294 | 194 | 6 | 92 | 382 | 591 | 876 | 5.48e-17 | 83.6 |
| MsG0880042738.01.T01 | MTR_3g011420 | 28.391 | 317 | 196 | 9 | 91 | 389 | 602 | 905 | 6.32e-17 | 83.6 |
| MsG0880042738.01.T01 | MTR_3g456080 | 33.074 | 257 | 149 | 9 | 107 | 354 | 47 | 289 | 9.30e-16 | 77.8 |
| MsG0880042738.01.T01 | MTR_5g027910 | 30.631 | 333 | 175 | 15 | 82 | 381 | 576 | 885 | 1.33e-15 | 79.3 |
| MsG0880042738.01.T01 | MTR_3g011270 | 29.618 | 314 | 196 | 10 | 91 | 389 | 600 | 903 | 2.38e-15 | 78.6 |
| MsG0880042738.01.T01 | MTR_3g011370 | 27.707 | 314 | 200 | 10 | 92 | 389 | 602 | 904 | 7.20e-15 | 77.0 |
| MsG0880042738.01.T01 | MTR_0691s0020 | 27.760 | 317 | 198 | 8 | 91 | 389 | 601 | 904 | 8.92e-15 | 77.0 |
| MsG0880042738.01.T01 | MTR_3g056410 | 26.935 | 323 | 199 | 10 | 92 | 396 | 562 | 865 | 1.38e-14 | 76.3 |
| MsG0880042738.01.T01 | MTR_3g056360 | 26.935 | 323 | 199 | 10 | 92 | 396 | 571 | 874 | 1.88e-14 | 75.9 |
| MsG0880042738.01.T01 | MTR_5g027810 | 28.000 | 300 | 180 | 8 | 110 | 389 | 607 | 890 | 2.18e-14 | 75.5 |
| MsG0880042738.01.T01 | MTR_3g012310 | 28.797 | 316 | 194 | 9 | 92 | 389 | 601 | 903 | 2.79e-14 | 75.5 |
| MsG0880042738.01.T01 | MTR_3g011460 | 28.931 | 318 | 193 | 9 | 91 | 389 | 600 | 903 | 3.99e-14 | 74.7 |
| MsG0880042738.01.T01 | MTR_3g011280 | 25.796 | 314 | 208 | 8 | 91 | 389 | 569 | 872 | 6.48e-14 | 74.3 |
| MsG0880042738.01.T01 | MTR_3g012250 | 27.832 | 309 | 191 | 8 | 92 | 381 | 606 | 901 | 6.62e-14 | 74.3 |
| MsG0880042738.01.T01 | MTR_3g011280 | 25.796 | 314 | 208 | 8 | 91 | 389 | 601 | 904 | 7.99e-14 | 73.9 |
| MsG0880042738.01.T01 | MTR_3g012060 | 28.939 | 311 | 200 | 7 | 92 | 389 | 597 | 899 | 9.87e-14 | 73.6 |
| MsG0880042738.01.T01 | MTR_3g011480 | 27.523 | 327 | 184 | 9 | 92 | 389 | 601 | 903 | 2.98e-13 | 72.0 |
| MsG0880042738.01.T01 | MTR_3g011300 | 27.900 | 319 | 194 | 9 | 91 | 389 | 599 | 901 | 4.68e-13 | 71.6 |
| MsG0880042738.01.T01 | MTR_3g012090 | 30.592 | 304 | 188 | 8 | 92 | 381 | 600 | 894 | 7.56e-12 | 67.8 |
| MsG0880042738.01.T01 | MTR_3g056625 | 27.365 | 296 | 199 | 7 | 92 | 378 | 285 | 573 | 4.58e-11 | 65.1 |
| MsG0880042738.01.T01 | MTR_1g078150 | 27.329 | 322 | 210 | 8 | 94 | 397 | 401 | 716 | 6.62e-11 | 64.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880042738.01.T01 | AT1G59780 | 29.373 | 303 | 187 | 9 | 91 | 389 | 589 | 868 | 7.82e-22 | 98.6 |
| MsG0880042738.01.T01 | AT1G59780 | 29.373 | 303 | 187 | 9 | 91 | 389 | 584 | 863 | 9.94e-22 | 98.2 |
| MsG0880042738.01.T01 | AT1G58400 | 27.451 | 306 | 183 | 9 | 92 | 380 | 588 | 871 | 2.88e-18 | 87.8 |
| MsG0880042738.01.T01 | AT1G58400 | 27.451 | 306 | 183 | 9 | 92 | 380 | 598 | 881 | 3.40e-18 | 87.4 |
| MsG0880042738.01.T01 | AT1G58400 | 27.451 | 306 | 183 | 9 | 92 | 380 | 598 | 881 | 3.40e-18 | 87.4 |
| MsG0880042738.01.T01 | AT1G58848 | 29.747 | 316 | 183 | 10 | 56 | 361 | 577 | 863 | 4.58e-17 | 84.0 |
| MsG0880042738.01.T01 | AT1G58848 | 34.426 | 183 | 108 | 6 | 186 | 367 | 795 | 966 | 1.96e-14 | 75.9 |
| MsG0880042738.01.T01 | AT1G59218 | 29.747 | 316 | 183 | 10 | 56 | 361 | 577 | 863 | 4.58e-17 | 84.0 |
| MsG0880042738.01.T01 | AT1G59218 | 34.426 | 183 | 108 | 6 | 186 | 367 | 795 | 966 | 1.96e-14 | 75.9 |
| MsG0880042738.01.T01 | AT1G59218 | 29.747 | 316 | 183 | 10 | 56 | 361 | 577 | 863 | 4.58e-17 | 84.0 |
| MsG0880042738.01.T01 | AT1G59218 | 34.426 | 183 | 108 | 6 | 186 | 367 | 795 | 966 | 1.96e-14 | 75.9 |
| MsG0880042738.01.T01 | AT1G59218 | 29.747 | 316 | 183 | 10 | 56 | 361 | 577 | 863 | 4.58e-17 | 84.0 |
| MsG0880042738.01.T01 | AT1G59218 | 34.426 | 183 | 108 | 6 | 186 | 367 | 795 | 966 | 1.96e-14 | 75.9 |
| MsG0880042738.01.T01 | AT1G58848 | 29.747 | 316 | 183 | 10 | 56 | 361 | 577 | 863 | 4.58e-17 | 84.0 |
| MsG0880042738.01.T01 | AT1G58848 | 34.426 | 183 | 108 | 6 | 186 | 367 | 795 | 966 | 1.96e-14 | 75.9 |
| MsG0880042738.01.T01 | AT5G35450 | 26.144 | 306 | 208 | 8 | 86 | 385 | 579 | 872 | 1.53e-15 | 79.3 |
| MsG0880042738.01.T01 | AT5G35450 | 26.144 | 306 | 208 | 8 | 86 | 385 | 579 | 872 | 1.53e-15 | 79.3 |
| MsG0880042738.01.T01 | AT5G48620 | 26.910 | 301 | 205 | 6 | 86 | 381 | 587 | 877 | 1.84e-15 | 79.0 |
| MsG0880042738.01.T01 | AT5G48620 | 26.910 | 301 | 205 | 6 | 86 | 381 | 587 | 877 | 1.84e-15 | 79.0 |
| MsG0880042738.01.T01 | AT5G48620 | 26.910 | 301 | 205 | 6 | 86 | 381 | 587 | 877 | 1.84e-15 | 79.0 |
| MsG0880042738.01.T01 | AT5G48620 | 26.910 | 301 | 205 | 6 | 86 | 381 | 587 | 877 | 1.84e-15 | 79.0 |
| MsG0880042738.01.T01 | AT5G48620 | 26.910 | 301 | 205 | 6 | 86 | 381 | 587 | 877 | 1.84e-15 | 79.0 |
| MsG0880042738.01.T01 | AT5G48620 | 26.910 | 301 | 205 | 6 | 86 | 381 | 587 | 877 | 1.84e-15 | 79.0 |
| MsG0880042738.01.T01 | AT1G53350 | 25.890 | 309 | 214 | 8 | 86 | 389 | 585 | 883 | 2.21e-15 | 79.0 |
| MsG0880042738.01.T01 | AT3G07040 | 29.545 | 308 | 198 | 10 | 91 | 389 | 595 | 892 | 5.41e-15 | 77.4 |
| MsG0880042738.01.T01 | AT1G59620 | 28.617 | 311 | 178 | 10 | 91 | 395 | 448 | 720 | 2.53e-14 | 75.5 |
| MsG0880042738.01.T01 | AT5G43470 | 26.934 | 349 | 221 | 9 | 47 | 385 | 557 | 881 | 3.54e-14 | 75.1 |
| MsG0880042738.01.T01 | AT5G43470 | 26.934 | 349 | 221 | 9 | 47 | 385 | 557 | 881 | 3.54e-14 | 75.1 |
| MsG0880042738.01.T01 | AT5G43470 | 26.934 | 349 | 221 | 9 | 47 | 385 | 557 | 881 | 3.54e-14 | 75.1 |
| MsG0880042738.01.T01 | AT5G43470 | 26.934 | 349 | 221 | 9 | 47 | 385 | 557 | 881 | 3.54e-14 | 75.1 |
| MsG0880042738.01.T01 | AT1G58390 | 27.797 | 295 | 197 | 8 | 86 | 378 | 595 | 875 | 5.57e-13 | 71.2 |
| MsG0880042738.01.T01 | AT1G58390 | 27.797 | 295 | 197 | 8 | 86 | 378 | 595 | 875 | 5.57e-13 | 71.2 |
| MsG0880042738.01.T01 | AT1G58807 | 35.514 | 107 | 68 | 1 | 273 | 378 | 863 | 969 | 1.05e-12 | 70.5 |
| MsG0880042738.01.T01 | AT1G58807 | 29.577 | 284 | 165 | 8 | 91 | 361 | 594 | 855 | 2.24e-11 | 66.2 |
| MsG0880042738.01.T01 | AT1G59124 | 35.514 | 107 | 68 | 1 | 273 | 378 | 863 | 969 | 1.05e-12 | 70.5 |
| MsG0880042738.01.T01 | AT1G59124 | 29.577 | 284 | 165 | 8 | 91 | 361 | 594 | 855 | 2.24e-11 | 66.2 |
| MsG0880042738.01.T01 | AT1G58602 | 37.864 | 103 | 60 | 4 | 273 | 374 | 869 | 968 | 1.62e-11 | 66.6 |
| MsG0880042738.01.T01 | AT1G58602 | 28.920 | 287 | 175 | 7 | 86 | 361 | 593 | 861 | 4.51e-11 | 65.5 |
| MsG0880042738.01.T01 | AT1G58602 | 37.864 | 103 | 60 | 4 | 273 | 374 | 869 | 968 | 1.62e-11 | 66.6 |
| MsG0880042738.01.T01 | AT1G58602 | 28.920 | 287 | 175 | 7 | 86 | 361 | 593 | 861 | 4.51e-11 | 65.5 |
Find 79 sgRNAs with CRISPR-Local
Find 98 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGTGCTAAGATTCTGGAATT+TGG | 0.216648 | 8:+13376573 | MsG0880042738.01.T01:CDS |
| TTGAGCTTACTTAAAGTCTT+AGG | 0.248252 | 8:-13375869 | None:intergenic |
| CGAAACTCTCCACACACTAT+TGG | 0.271255 | 8:+13376102 | MsG0880042738.01.T01:CDS |
| ATTCTGGAATTTGGAGAATC+TGG | 0.291131 | 8:+13376582 | MsG0880042738.01.T01:CDS |
| ATTTCCTTATCCTCTGGAAT+AGG | 0.296405 | 8:-13375737 | None:intergenic |
| GAAACTCTCCACACACTATT+GGG | 0.304239 | 8:+13376103 | MsG0880042738.01.T01:CDS |
| ATATCGTAAGAAGGCGTTAT+TGG | 0.306723 | 8:+13376765 | MsG0880042738.01.T01:CDS |
| TCTGGAAGAATGGGATATTA+AGG | 0.313790 | 8:+13376600 | MsG0880042738.01.T01:CDS |
| GAAATCAATGTGTGATGAAT+TGG | 0.321474 | 8:+13375832 | MsG0880042738.01.T01:CDS |
| CTCTCATCCATCCGGCTGTT+TGG | 0.330032 | 8:+13376355 | MsG0880042738.01.T01:CDS |
| TTTCCTTATCCTCTGGAATA+GGG | 0.349768 | 8:-13375736 | None:intergenic |
| CATGCCTTCAACACTTATAA+TGG | 0.360369 | 8:-13375768 | None:intergenic |
| CCGGCTGTTTGGAAGATTAG+AGG | 0.363662 | 8:+13376366 | MsG0880042738.01.T01:CDS |
| ATTACATCCCTAGCTCAATC+TGG | 0.372209 | 8:+13375999 | MsG0880042738.01.T01:CDS |
| AAATTAGCTTTCCAATTGAA+TGG | 0.384065 | 8:+13376193 | MsG0880042738.01.T01:CDS |
| AACTTCAAGTGCTAAGATTC+TGG | 0.418353 | 8:+13376566 | MsG0880042738.01.T01:CDS |
| ATTTGGAGAATCTGGAAGAA+TGG | 0.418360 | 8:+13376590 | MsG0880042738.01.T01:CDS |
| CTAGCTCTGGTCCATTCAAT+TGG | 0.421788 | 8:-13376204 | None:intergenic |
| CCCATGCATATGCAAACTTC+GGG | 0.425126 | 8:+13375940 | MsG0880042738.01.T01:CDS |
| TTTGGAGAATCTGGAAGAAT+GGG | 0.434678 | 8:+13376591 | MsG0880042738.01.T01:CDS |
| TGAAGGCATGCTGAGACATC+TGG | 0.439440 | 8:+13375781 | MsG0880042738.01.T01:CDS |
| GATGTGATTTCCTTATCCTC+TGG | 0.444601 | 8:-13375743 | None:intergenic |
| CTTCAAATGCTTCAATCCAC+TGG | 0.445523 | 8:-13376683 | None:intergenic |
| TTTAATTCCCAGATTGAGCT+AGG | 0.454313 | 8:-13376007 | None:intergenic |
| AAGTTAAGTTGTACTTGAGC+AGG | 0.459561 | 8:-13375679 | None:intergenic |
| ATGAGCAGGTTCTTCAACTC+TGG | 0.461661 | 8:-13376469 | None:intergenic |
| CATATGCAAACTTCGGGAGC+TGG | 0.463470 | 8:+13375946 | MsG0880042738.01.T01:CDS |
| AGCTCGGTCCTGCATAAACT+TGG | 0.465244 | 8:+13376654 | MsG0880042738.01.T01:CDS |
| TAGCTTAAGATGGACATACT+TGG | 0.469064 | 8:+13375907 | MsG0880042738.01.T01:CDS |
| TCAGCCATTATAAGTGTTGA+AGG | 0.473575 | 8:+13375764 | MsG0880042738.01.T01:CDS |
| TCGAGTATCTTAGCTTAAGA+TGG | 0.477896 | 8:+13375897 | MsG0880042738.01.T01:CDS |
| CTGAATTGACGTGTAACAAA+GGG | 0.478051 | 8:-13375620 | None:intergenic |
| CCCCATGCATATGCAAACTT+CGG | 0.479746 | 8:+13375939 | MsG0880042738.01.T01:CDS |
| GTGTCTTGCAGCTCTAGCTC+TGG | 0.482624 | 8:-13376217 | None:intergenic |
| CTTGAGCAGGTTATTAAACT+CGG | 0.494802 | 8:-13375666 | None:intergenic |
| CCTCTAATCTTCCAAACAGC+CGG | 0.508329 | 8:-13376366 | None:intergenic |
| CCCGAAGTTTGCATATGCAT+GGG | 0.514502 | 8:-13375940 | None:intergenic |
| AAATATCGAAGTAAACTTGA+GGG | 0.522018 | 8:+13376058 | MsG0880042738.01.T01:CDS |
| CTTAAATCAGTGAATGAAAG+TGG | 0.522579 | 8:+13376286 | MsG0880042738.01.T01:CDS |
| TCCCGAAGTTTGCATATGCA+TGG | 0.522995 | 8:-13375941 | None:intergenic |
| TGAACAATCTCTCATCCATC+CGG | 0.530645 | 8:+13376347 | MsG0880042738.01.T01:CDS |
| TTCCTTATCCTCTGGAATAG+GGG | 0.535404 | 8:-13375735 | None:intergenic |
| GCTTCAAATTCCACGAGGCT+AGG | 0.535501 | 8:-13376634 | None:intergenic |
| TGTTACACGTCAATTCAGAA+AGG | 0.539993 | 8:+13375625 | MsG0880042738.01.T01:CDS |
| CATGTGGTTATATCGTAAGA+AGG | 0.543409 | 8:+13376756 | MsG0880042738.01.T01:CDS |
| TCCTTATCCTCTGGAATAGG+GGG | 0.543459 | 8:-13375734 | None:intergenic |
| TTACATCCCTAGCTCAATCT+GGG | 0.552502 | 8:+13376000 | MsG0880042738.01.T01:CDS |
| AGATGGATTGTGCCTCGGGA+GGG | 0.557862 | 8:+13376536 | MsG0880042738.01.T01:CDS |
| GGGAAATGCCAAGTTTATGC+AGG | 0.561009 | 8:-13376662 | None:intergenic |
| ACGTCAATTCAGAAAGGAGA+TGG | 0.561140 | 8:+13375631 | MsG0880042738.01.T01:CDS |
| TCGGAAGGAGGCTCATTCGA+AGG | 0.564124 | 8:-13376393 | None:intergenic |
| AATCTTAGCACTTGAAGTTG+CGG | 0.565476 | 8:-13376562 | None:intergenic |
| TTAATTCCCAGATTGAGCTA+GGG | 0.566724 | 8:-13376006 | None:intergenic |
| CTTAAAGTCTTAGGTAACTG+TGG | 0.567491 | 8:-13375860 | None:intergenic |
| AGGTTCTTCAACTCTGGCAT+AGG | 0.568213 | 8:-13376463 | None:intergenic |
| GAAGGCTCTTCAGCTTGTGA+AGG | 0.568416 | 8:-13376162 | None:intergenic |
| AAACTCTCCACACACTATTG+GGG | 0.570283 | 8:+13376104 | MsG0880042738.01.T01:CDS |
| GCCTCGTGGAATTTGAAGCT+CGG | 0.573600 | 8:+13376638 | MsG0880042738.01.T01:CDS |
| AAAATATCGAAGTAAACTTG+AGG | 0.575643 | 8:+13376057 | MsG0880042738.01.T01:CDS |
| TCTGAATTGACGTGTAACAA+AGG | 0.579180 | 8:-13375621 | None:intergenic |
| ACAATGACTTAAGCATGAGC+AGG | 0.583112 | 8:-13376483 | None:intergenic |
| TTCAAATGCTTCAATCCACT+GGG | 0.586056 | 8:-13376682 | None:intergenic |
| GAAGAATGGGATATTAAGGA+AGG | 0.590714 | 8:+13376604 | MsG0880042738.01.T01:CDS |
| TAATCTTCCAAACAGCCGGA+TGG | 0.590936 | 8:-13376362 | None:intergenic |
| TTGTGAAGGTAATCAAGAAG+TGG | 0.592412 | 8:-13376148 | None:intergenic |
| TGTGAAGGTAATCAAGAAGT+GGG | 0.596301 | 8:-13376147 | None:intergenic |
| AAGATGGATTGTGCCTCGGG+AGG | 0.606200 | 8:+13376535 | MsG0880042738.01.T01:CDS |
| CCGAAGTTTGCATATGCATG+GGG | 0.608821 | 8:-13375939 | None:intergenic |
| AAACTTGAGGGCAAGCCGAG+TGG | 0.632080 | 8:+13376070 | MsG0880042738.01.T01:CDS |
| ACTGATTTAAGTGTCAGTGA+AGG | 0.635781 | 8:-13376274 | None:intergenic |
| AACTCTCCACACACTATTGG+GGG | 0.640436 | 8:+13376105 | MsG0880042738.01.T01:CDS |
| AGGAGCAATGCCTAGCCTCG+TGG | 0.648362 | 8:+13376624 | MsG0880042738.01.T01:CDS |
| GTTTCGTTAAAGTTTCCACT+CGG | 0.649646 | 8:-13376085 | None:intergenic |
| CCTTATCCTCTGGAATAGGG+GGG | 0.651072 | 8:-13375733 | None:intergenic |
| CATGTTGAAGAAATTCACGA+TGG | 0.677175 | 8:+13375592 | None:intergenic |
| CGAAGTTTGCATATGCATGG+GGG | 0.707287 | 8:-13375938 | None:intergenic |
| AAGTTGCGGAAACCCTCCCG+AGG | 0.707957 | 8:-13376548 | None:intergenic |
| ATAAACTTGGCATTTCCCAG+TGG | 0.733563 | 8:+13376667 | MsG0880042738.01.T01:CDS |
| ACCGAGCTTCAAATTCCACG+AGG | 0.783000 | 8:-13376639 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AAGCTAATTTCAAATTTTGA+AGG | - | Chr8:13376183-13376202 | None:intergenic | 20.0% |
| !!! | AAGTTTACTTCGATATTTTT+GGG | - | Chr8:13376056-13376075 | None:intergenic | 20.0% |
| ! | AAAATATCGAAGTAAACTTG+AGG | + | Chr8:13376057-13376076 | MsG0880042738.01.T01:CDS | 25.0% |
| ! | AAATATCGAAGTAAACTTGA+GGG | + | Chr8:13376058-13376077 | MsG0880042738.01.T01:CDS | 25.0% |
| !! | CAAGTTTACTTCGATATTTT+TGG | - | Chr8:13376057-13376076 | None:intergenic | 25.0% |
| !! | TTTTACGTAACTTTATCGTT+CGG | - | Chr8:13376714-13376733 | None:intergenic | 25.0% |
| !!! | AAATTAGCTTTCCAATTGAA+TGG | + | Chr8:13376193-13376212 | MsG0880042738.01.T01:CDS | 25.0% |
| !!! | AGTTTACTTCGATATTTTTG+GGG | - | Chr8:13376055-13376074 | None:intergenic | 25.0% |
| CTTAAATCAGTGAATGAAAG+TGG | + | Chr8:13376286-13376305 | MsG0880042738.01.T01:CDS | 30.0% | |
| GAAATCAATGTGTGATGAAT+TGG | + | Chr8:13375832-13375851 | MsG0880042738.01.T01:CDS | 30.0% | |
| TTGAGCTTACTTAAAGTCTT+AGG | - | Chr8:13375872-13375891 | None:intergenic | 30.0% | |
| ! | GTGAGATCAATGAGATTTTT+CGG | - | Chr8:13376415-13376434 | None:intergenic | 30.0% |
| AACTTCAAGTGCTAAGATTC+TGG | + | Chr8:13376566-13376585 | MsG0880042738.01.T01:CDS | 35.0% | |
| AATCTTAGCACTTGAAGTTG+CGG | - | Chr8:13376565-13376584 | None:intergenic | 35.0% | |
| ATCCATTCGTGAAAAACATG+TGG | + | Chr8:13376740-13376759 | MsG0880042738.01.T01:CDS | 35.0% | |
| ATTTGGAGAATCTGGAAGAA+TGG | + | Chr8:13376590-13376609 | MsG0880042738.01.T01:CDS | 35.0% | |
| CATGCCTTCAACACTTATAA+TGG | - | Chr8:13375771-13375790 | None:intergenic | 35.0% | |
| CATGTGGTTATATCGTAAGA+AGG | + | Chr8:13376756-13376775 | MsG0880042738.01.T01:CDS | 35.0% | |
| CTCTTGTACAGACAAAAAGA+TGG | + | Chr8:13376519-13376538 | MsG0880042738.01.T01:CDS | 35.0% | |
| CTGAATTGACGTGTAACAAA+GGG | - | Chr8:13375623-13375642 | None:intergenic | 35.0% | |
| CTTAAAGTCTTAGGTAACTG+TGG | - | Chr8:13375863-13375882 | None:intergenic | 35.0% | |
| CTTGAGCAGGTTATTAAACT+CGG | - | Chr8:13375669-13375688 | None:intergenic | 35.0% | |
| GAAGAATGGGATATTAAGGA+AGG | + | Chr8:13376604-13376623 | MsG0880042738.01.T01:CDS | 35.0% | |
| GTTTCGTTAAAGTTTCCACT+CGG | - | Chr8:13376088-13376107 | None:intergenic | 35.0% | |
| TAGCTTAAGATGGACATACT+TGG | + | Chr8:13375907-13375926 | MsG0880042738.01.T01:CDS | 35.0% | |
| TCGAGTATCTTAGCTTAAGA+TGG | + | Chr8:13375897-13375916 | MsG0880042738.01.T01:CDS | 35.0% | |
| TCTGAATTGACGTGTAACAA+AGG | - | Chr8:13375624-13375643 | None:intergenic | 35.0% | |
| TCTGGAAGAATGGGATATTA+AGG | + | Chr8:13376600-13376619 | MsG0880042738.01.T01:CDS | 35.0% | |
| TGTGAAGGTAATCAAGAAGT+GGG | - | Chr8:13376150-13376169 | None:intergenic | 35.0% | |
| TGTTACACGTCAATTCAGAA+AGG | + | Chr8:13375625-13375644 | MsG0880042738.01.T01:CDS | 35.0% | |
| TTAATTCCCAGATTGAGCTA+GGG | - | Chr8:13376009-13376028 | None:intergenic | 35.0% | |
| TTCAAATGCTTCAATCCACT+GGG | - | Chr8:13376685-13376704 | None:intergenic | 35.0% | |
| TTGTGAAGGTAATCAAGAAG+TGG | - | Chr8:13376151-13376170 | None:intergenic | 35.0% | |
| TTTAATTCCCAGATTGAGCT+AGG | - | Chr8:13376010-13376029 | None:intergenic | 35.0% | |
| TTTGGAGAATCTGGAAGAAT+GGG | + | Chr8:13376591-13376610 | MsG0880042738.01.T01:CDS | 35.0% | |
| ! | AACCACATGTTTTTCACGAA+TGG | - | Chr8:13376745-13376764 | None:intergenic | 35.0% |
| ! | AAGTTAAGTTGTACTTGAGC+AGG | - | Chr8:13375682-13375701 | None:intergenic | 35.0% |
| ! | ACTGATTTAAGTGTCAGTGA+AGG | - | Chr8:13376277-13376296 | None:intergenic | 35.0% |
| !! | AGTGCTAAGATTCTGGAATT+TGG | + | Chr8:13376573-13376592 | MsG0880042738.01.T01:CDS | 35.0% |
| !! | ATATCGTAAGAAGGCGTTAT+TGG | + | Chr8:13376765-13376784 | MsG0880042738.01.T01:CDS | 35.0% |
| !! | ATTCTGGAATTTGGAGAATC+TGG | + | Chr8:13376582-13376601 | MsG0880042738.01.T01:CDS | 35.0% |
| !! | ATTTCCTTATCCTCTGGAAT+AGG | - | Chr8:13375740-13375759 | None:intergenic | 35.0% |
| !! | GATCAATGAGATTTTTCGGA+AGG | - | Chr8:13376411-13376430 | None:intergenic | 35.0% |
| !! | TCAGCCATTATAAGTGTTGA+AGG | + | Chr8:13375764-13375783 | MsG0880042738.01.T01:CDS | 35.0% |
| !! | TTTCCTTATCCTCTGGAATA+GGG | - | Chr8:13375739-13375758 | None:intergenic | 35.0% |
| !!! | TGATAGTTTTGAAGCTGACA+AGG | - | Chr8:13376437-13376456 | None:intergenic | 35.0% |
| AAACTCTCCACACACTATTG+GGG | + | Chr8:13376104-13376123 | MsG0880042738.01.T01:CDS | 40.0% | |
| ACAATGACTTAAGCATGAGC+AGG | - | Chr8:13376486-13376505 | None:intergenic | 40.0% | |
| ACGTCAATTCAGAAAGGAGA+TGG | + | Chr8:13375631-13375650 | MsG0880042738.01.T01:CDS | 40.0% | |
| ATAAACTTGGCATTTCCCAG+TGG | + | Chr8:13376667-13376686 | MsG0880042738.01.T01:CDS | 40.0% | |
| ATTACATCCCTAGCTCAATC+TGG | + | Chr8:13375999-13376018 | MsG0880042738.01.T01:CDS | 40.0% | |
| CTTCAAATGCTTCAATCCAC+TGG | - | Chr8:13376686-13376705 | None:intergenic | 40.0% | |
| GAAACTCTCCACACACTATT+GGG | + | Chr8:13376103-13376122 | MsG0880042738.01.T01:CDS | 40.0% | |
| GATGTGATTTCCTTATCCTC+TGG | - | Chr8:13375746-13375765 | None:intergenic | 40.0% | |
| TGAACAATCTCTCATCCATC+CGG | + | Chr8:13376347-13376366 | MsG0880042738.01.T01:CDS | 40.0% | |
| TTACATCCCTAGCTCAATCT+GGG | + | Chr8:13376000-13376019 | MsG0880042738.01.T01:CDS | 40.0% | |
| !! | AAAAAGATGGATTGTGCCTC+GGG | + | Chr8:13376532-13376551 | MsG0880042738.01.T01:CDS | 40.0% |
| !! | CAAAAAGATGGATTGTGCCT+CGG | + | Chr8:13376531-13376550 | MsG0880042738.01.T01:CDS | 40.0% |
| !! | CAATGAGATTTTTCGGAAGG+AGG | - | Chr8:13376408-13376427 | None:intergenic | 40.0% |
| !! | TATTGGGGGTGTTCTTGTAT+GGG | + | Chr8:13376119-13376138 | MsG0880042738.01.T01:CDS | 40.0% |
| !! | TTCCTTATCCTCTGGAATAG+GGG | - | Chr8:13375738-13375757 | None:intergenic | 40.0% |
| AACTCTCCACACACTATTGG+GGG | + | Chr8:13376105-13376124 | MsG0880042738.01.T01:CDS | 45.0% | |
| AGGTTCTTCAACTCTGGCAT+AGG | - | Chr8:13376466-13376485 | None:intergenic | 45.0% | |
| ATGAGCAGGTTCTTCAACTC+TGG | - | Chr8:13376472-13376491 | None:intergenic | 45.0% | |
| CCCATGCATATGCAAACTTC+GGG | + | Chr8:13375940-13375959 | MsG0880042738.01.T01:CDS | 45.0% | |
| CCCCATGCATATGCAAACTT+CGG | + | Chr8:13375939-13375958 | MsG0880042738.01.T01:CDS | 45.0% | |
| CCCGAAGTTTGCATATGCAT+GGG | - | Chr8:13375943-13375962 | None:intergenic | 45.0% | |
| CCGAAGTTTGCATATGCATG+GGG | - | Chr8:13375942-13375961 | None:intergenic | 45.0% | |
| CCTCTAATCTTCCAAACAGC+CGG | - | Chr8:13376369-13376388 | None:intergenic | 45.0% | |
| CGAAACTCTCCACACACTAT+TGG | + | Chr8:13376102-13376121 | MsG0880042738.01.T01:CDS | 45.0% | |
| CGAAGTTTGCATATGCATGG+GGG | - | Chr8:13375941-13375960 | None:intergenic | 45.0% | |
| GGGAAATGCCAAGTTTATGC+AGG | - | Chr8:13376665-13376684 | None:intergenic | 45.0% | |
| TAATCTTCCAAACAGCCGGA+TGG | - | Chr8:13376365-13376384 | None:intergenic | 45.0% | |
| TCCCGAAGTTTGCATATGCA+TGG | - | Chr8:13375944-13375963 | None:intergenic | 45.0% | |
| !! | CTAGCTCTGGTCCATTCAAT+TGG | - | Chr8:13376207-13376226 | None:intergenic | 45.0% |
| !! | CTATTGGGGGTGTTCTTGTA+TGG | + | Chr8:13376118-13376137 | MsG0880042738.01.T01:CDS | 45.0% |
| !! | TCCTTATCCTCTGGAATAGG+GGG | - | Chr8:13375737-13375756 | None:intergenic | 45.0% |
| ACCGAGCTTCAAATTCCACG+AGG | - | Chr8:13376642-13376661 | None:intergenic | 50.0% | |
| AGAACACCCCCAATAGTGTG+TGG | - | Chr8:13376114-13376133 | None:intergenic | 50.0% | |
| AGCTCGGTCCTGCATAAACT+TGG | + | Chr8:13376654-13376673 | MsG0880042738.01.T01:CDS | 50.0% | |
| CATATGCAAACTTCGGGAGC+TGG | + | Chr8:13375946-13375965 | MsG0880042738.01.T01:CDS | 50.0% | |
| CCGGCTGTTTGGAAGATTAG+AGG | + | Chr8:13376366-13376385 | MsG0880042738.01.T01:CDS | 50.0% | |
| GAAGGCTCTTCAGCTTGTGA+AGG | - | Chr8:13376165-13376184 | None:intergenic | 50.0% | |
| GCTTCAAATTCCACGAGGCT+AGG | - | Chr8:13376637-13376656 | None:intergenic | 50.0% | |
| ! | GCCTCGTGGAATTTGAAGCT+CGG | + | Chr8:13376638-13376657 | MsG0880042738.01.T01:CDS | 50.0% |
| ! | TGAAGGCATGCTGAGACATC+TGG | + | Chr8:13375781-13375800 | MsG0880042738.01.T01:CDS | 50.0% |
| !! | CCTTATCCTCTGGAATAGGG+GGG | - | Chr8:13375736-13375755 | None:intergenic | 50.0% |
| AAACTTGAGGGCAAGCCGAG+TGG | + | Chr8:13376070-13376089 | MsG0880042738.01.T01:CDS | 55.0% | |
| AACCATCCCCCCTATTCCAG+AGG | + | Chr8:13375727-13375746 | MsG0880042738.01.T01:CDS | 55.0% | |
| CCCCCCTATTCCAGAGGATA+AGG | + | Chr8:13375733-13375752 | MsG0880042738.01.T01:CDS | 55.0% | |
| CTCTCATCCATCCGGCTGTT+TGG | + | Chr8:13376355-13376374 | MsG0880042738.01.T01:CDS | 55.0% | |
| TCGGAAGGAGGCTCATTCGA+AGG | - | Chr8:13376396-13376415 | None:intergenic | 55.0% | |
| ! | GTGTCTTGCAGCTCTAGCTC+TGG | - | Chr8:13376220-13376239 | None:intergenic | 55.0% |
| !! | AAGATGGATTGTGCCTCGGG+AGG | + | Chr8:13376535-13376554 | MsG0880042738.01.T01:CDS | 55.0% |
| !! | AGATGGATTGTGCCTCGGGA+GGG | + | Chr8:13376536-13376555 | MsG0880042738.01.T01:CDS | 55.0% |
| !! | ATCCTCTGGAATAGGGGGGA+TGG | - | Chr8:13375732-13375751 | None:intergenic | 55.0% |
| AAGTTGCGGAAACCCTCCCG+AGG | - | Chr8:13376551-13376570 | None:intergenic | 60.0% | |
| AGGAGCAATGCCTAGCCTCG+TGG | + | Chr8:13376624-13376643 | MsG0880042738.01.T01:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr8 | gene | 13375593 | 13376807 | 13375593 | ID=MsG0880042738.01;Name=MsG0880042738.01 |
| Chr8 | mRNA | 13375593 | 13376807 | 13375593 | ID=MsG0880042738.01.T01;Parent=MsG0880042738.01;Name=MsG0880042738.01.T01;_AED=0.16;_eAED=0.17;_QI=0|-1|0|1|-1|1|1|0|404 |
| Chr8 | exon | 13375593 | 13376807 | 13375593 | ID=MsG0880042738.01.T01:exon:828;Parent=MsG0880042738.01.T01 |
| Chr8 | CDS | 13375593 | 13376807 | 13375593 | ID=MsG0880042738.01.T01:cds;Parent=MsG0880042738.01.T01 |
| Gene Sequence |
| Protein sequence |
