AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0780036960.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780036960.01.T01 MTR_7g024520 89.583 96 9 1 3 97 7 102 3.95e-57 179
MsG0780036960.01.T01 MTR_7g024500 90.526 95 8 1 4 97 8 102 4.26e-57 179
MsG0780036960.01.T01 MTR_4g088345 55.789 95 41 1 4 97 9 103 3.92e-34 120
MsG0780036960.01.T01 MTR_1g076940 48.148 108 48 2 4 103 9 116 2.55e-29 107
MsG0780036960.01.T01 MTR_7g014570 51.064 94 45 1 4 97 9 101 6.83e-28 103
MsG0780036960.01.T01 MTR_7g014510 47.959 98 47 1 4 97 9 106 4.35e-26 99.4
MsG0780036960.01.T01 MTR_4g088340 53.750 80 36 1 19 97 1 80 8.24e-26 98.2
MsG0780036960.01.T01 MTR_4g033170 42.105 95 55 0 3 97 8 102 6.03e-23 90.9
MsG0780036960.01.T01 MTR_4g033085 42.105 95 55 0 3 97 8 102 8.91e-23 90.5
MsG0780036960.01.T01 MTR_1g076930 48.352 91 41 2 19 103 1 91 4.41e-22 87.0
MsG0780036960.01.T01 MTR_5g074680 40.816 98 55 1 3 97 9 106 6.52e-22 88.2
MsG0780036960.01.T01 MTR_4g088200 43.434 99 52 2 3 97 8 106 1.61e-21 87.0
MsG0780036960.01.T01 MTR_4g033015 40.000 100 54 1 3 96 8 107 3.33e-21 86.3
MsG0780036960.01.T01 MTR_4g033150 39.000 100 55 1 3 96 8 107 8.93e-21 85.1
MsG0780036960.01.T01 MTR_0794s0020 39.252 107 53 3 6 100 11 117 9.42e-21 83.6
MsG0780036960.01.T01 MTR_4g088190 44.118 102 50 2 3 97 8 109 9.47e-21 85.1
MsG0780036960.01.T01 MTR_4g033200 39.000 100 55 1 3 96 8 107 1.13e-20 84.7
MsG0780036960.01.T01 MTR_4g079160 38.947 95 58 0 3 97 7 101 9.25e-20 82.4
MsG0780036960.01.T01 MTR_5g074580 40.698 86 51 0 3 88 9 94 1.54e-19 81.6
MsG0780036960.01.T01 MTR_5g074600 40.625 96 54 1 5 97 10 105 2.70e-19 80.9
MsG0780036960.01.T01 MTR_5g074540 35.870 92 59 0 3 94 8 99 6.68e-18 77.0
MsG0780036960.01.T01 MTR_5g074610 37.662 77 48 0 3 79 4 80 3.28e-16 72.4
MsG0780036960.01.T01 MTR_4g025570 35.417 96 49 3 10 99 22 110 1.93e-11 58.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 16 sgRNAs with CRISPR-Local

Find 19 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAATAGGTTCGCCATGATTT+TGG 0.194358 7:+17165656 None:intergenic
TCACAGTGTCATGCGTTTAT+TGG 0.252473 7:-17165578 MsG0780036960.01.T01:CDS
ACAAGCTCAGGAAGAGTAAT+AGG 0.291026 7:+17165640 None:intergenic
TACTCTTCCTGAGCTTGTCT+CGG 0.435364 7:-17165635 MsG0780036960.01.T01:CDS
CATGACACTGTGAACACAAC+CGG 0.451299 7:+17165588 None:intergenic
ATCAAAAGCTTCTTGTGAAA+GGG 0.462474 7:-17165491 MsG0780036960.01.T01:CDS
ATCAAGTGGGCTGTTGAACT+TGG 0.469328 7:-17165694 MsG0780036960.01.T01:CDS
CATCAAAAGCTTCTTGTGAA+AGG 0.493090 7:-17165492 MsG0780036960.01.T01:CDS
CGAAGAGCCGAGACAAGCTC+AGG 0.517956 7:+17165628 None:intergenic
AATCTTCGCAATTGTCAAGA+TGG 0.519987 7:-17165545 MsG0780036960.01.T01:CDS
CCAGATATAATCCAAAATCA+TGG 0.539133 7:-17165667 MsG0780036960.01.T01:CDS
TGAGACCCGCGTCGATCAAG+TGG 0.547754 7:-17165708 MsG0780036960.01.T01:CDS
CGAATTCCAGAAGCAAAAGC+CGG 0.553157 7:-17165607 MsG0780036960.01.T01:CDS
ACAGCCCACTTGATCGACGC+GGG 0.642324 7:+17165703 None:intergenic
GAGACCCGCGTCGATCAAGT+GGG 0.673235 7:-17165707 MsG0780036960.01.T01:CDS
AACAGCCCACTTGATCGACG+CGG 0.727407 7:+17165702 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
ATCAAAAGCTTCTTGTGAAA+GGG - Chr7:17165770-17165789 MsG0780036960.01.T01:CDS 30.0%
CCAGATATAATCCAAAATCA+TGG - Chr7:17165594-17165613 MsG0780036960.01.T01:CDS 30.0%
! CCATGATTTTGGATTATATC+TGG + Chr7:17165597-17165616 None:intergenic 30.0%
AATCTTCGCAATTGTCAAGA+TGG - Chr7:17165716-17165735 MsG0780036960.01.T01:CDS 35.0%
CATCAAAAGCTTCTTGTGAA+AGG - Chr7:17165769-17165788 MsG0780036960.01.T01:CDS 35.0%
! TAATAGGTTCGCCATGATTT+TGG + Chr7:17165608-17165627 None:intergenic 35.0%
! TCACAAGAAGCTTTTGATGT+TGG + Chr7:17165768-17165787 None:intergenic 35.0%
ACAAGCTCAGGAAGAGTAAT+AGG + Chr7:17165624-17165643 None:intergenic 40.0%
TCACAGTGTCATGCGTTTAT+TGG - Chr7:17165683-17165702 MsG0780036960.01.T01:CDS 40.0%
CATGACACTGTGAACACAAC+CGG + Chr7:17165676-17165695 None:intergenic 45.0%
CGAATTCCAGAAGCAAAAGC+CGG - Chr7:17165654-17165673 MsG0780036960.01.T01:CDS 45.0%
TACTCTTCCTGAGCTTGTCT+CGG - Chr7:17165626-17165645 MsG0780036960.01.T01:CDS 45.0%
!! ATCAAGTGGGCTGTTGAACT+TGG - Chr7:17165567-17165586 MsG0780036960.01.T01:CDS 45.0%
!! ACACAACCGGCTTTTGCTTC+TGG + Chr7:17165663-17165682 None:intergenic 50.0%
AACAGCCCACTTGATCGACG+CGG + Chr7:17165562-17165581 None:intergenic 55.0%
ACAGCCCACTTGATCGACGC+GGG + Chr7:17165561-17165580 None:intergenic 60.0%
CGAAGAGCCGAGACAAGCTC+AGG + Chr7:17165636-17165655 None:intergenic 60.0%
! GAGACCCGCGTCGATCAAGT+GGG - Chr7:17165554-17165573 MsG0780036960.01.T01:CDS 60.0%
! TGAGACCCGCGTCGATCAAG+TGG - Chr7:17165553-17165572 MsG0780036960.01.T01:CDS 60.0%
Chromosome Type Strat End Strand Name
Chr7 gene 17165486 17165797 17165486 ID=MsG0780036960.01;Name=MsG0780036960.01
Chr7 mRNA 17165486 17165797 17165486 ID=MsG0780036960.01.T01;Parent=MsG0780036960.01;Name=MsG0780036960.01.T01;_AED=0.36;_eAED=0.38;_QI=0|-1|0|1|-1|1|1|0|103
Chr7 exon 17165486 17165797 17165486 ID=MsG0780036960.01.T01:exon:3183;Parent=MsG0780036960.01.T01
Chr7 CDS 17165486 17165797 17165486 ID=MsG0780036960.01.T01:cds;Parent=MsG0780036960.01.T01
Gene Sequence

>MsG0780036960.01.T01

ATGAGAAACAAAGCAAGTGAGCTCTTTGAAGCTCAAGCTCACTTGTACAATCAGTTATTCAGCTTCATGAGACCCGCGTCGATCAAGTGGGCTGTTGAACTTGGCATACCAGATATAATCCAAAATCATGGCGAACCTATTACTCTTCCTGAGCTTGTCTCGGCTCTTCGAATTCCAGAAGCAAAAGCCGGTTGTGTTCACAGTGTCATGCGTTTATTGGCGCACAATAAAATCTTCGCAATTGTCAAGATGGCACAACAAGAAGCATATGCTCTTTCTCCAACATCAAAAGCTTCTTGTGAAAGGGACTGA

Protein sequence

>MsG0780036960.01.T01

MRNKASELFEAQAHLYNQLFSFMRPASIKWAVELGIPDIIQNHGEPITLPELVSALRIPEAKAGCVHSVMRLLAHNKIFAIVKMAQQEAYALSPTSKASCERD*