Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880042953.01.T01 | AET01987.1 | 82.988 | 241 | 18 | 3 | 1 | 218 | 1 | 241 | 6.45E-125 | 363 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880042953.01.T01 | P46897 | 40.316 | 253 | 108 | 8 | 8 | 218 | 7 | 258 | 1.03E-38 | 137 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880042953.01.T01 | G7LAS8 | 82.988 | 241 | 18 | 3 | 1 | 218 | 1 | 241 | 3.08e-125 | 363 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180004536.01 | MsG0880042953.01 | 0.810544 | 1.093583e-50 | 6.610377e-48 |
| MsG0880042953.01 | MsG0880044354.01 | 0.836756 | 7.767090e-57 | 9.882615e-54 |
| MsG0280010945.01 | MsG0880042953.01 | 0.804081 | 2.562894e-49 | 1.309418e-46 |
| MsG0380016055.01 | MsG0880042953.01 | 0.823397 | 1.416243e-53 | 1.217860e-50 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880042953.01.T01 | MTR_8g026960 | 82.988 | 241 | 18 | 3 | 1 | 218 | 1 | 241 | 7.81e-129 | 363 |
| MsG0880042953.01.T01 | MTR_3g092150 | 34.951 | 206 | 112 | 5 | 35 | 218 | 40 | 245 | 2.48e-26 | 102 |
| MsG0880042953.01.T01 | MTR_8g089895 | 41.176 | 102 | 49 | 2 | 35 | 125 | 56 | 157 | 4.07e-16 | 75.9 |
| MsG0880042953.01.T01 | MTR_5g038280 | 65.957 | 47 | 16 | 0 | 35 | 81 | 89 | 135 | 5.19e-15 | 72.8 |
| MsG0880042953.01.T01 | MTR_8g468210 | 45.679 | 81 | 39 | 1 | 1 | 81 | 50 | 125 | 1.48e-14 | 71.2 |
| MsG0880042953.01.T01 | MTR_6g011610 | 65.957 | 47 | 16 | 0 | 35 | 81 | 98 | 144 | 1.77e-14 | 70.9 |
| MsG0880042953.01.T01 | MTR_6g011610 | 65.957 | 47 | 16 | 0 | 35 | 81 | 79 | 125 | 1.83e-14 | 70.5 |
| MsG0880042953.01.T01 | MTR_5g039000 | 46.053 | 76 | 41 | 0 | 6 | 81 | 50 | 125 | 3.42e-14 | 70.1 |
| MsG0880042953.01.T01 | MTR_3g080100 | 37.037 | 108 | 57 | 2 | 35 | 131 | 22 | 129 | 3.82e-14 | 68.9 |
| MsG0880042953.01.T01 | MTR_3g080100 | 37.037 | 108 | 57 | 2 | 35 | 131 | 61 | 168 | 4.82e-14 | 69.3 |
| MsG0880042953.01.T01 | MTR_8g469430 | 61.702 | 47 | 18 | 0 | 35 | 81 | 91 | 137 | 5.49e-14 | 69.7 |
| MsG0880042953.01.T01 | MTR_1g061660 | 50.000 | 56 | 28 | 0 | 26 | 81 | 52 | 107 | 1.15e-13 | 68.2 |
| MsG0880042953.01.T01 | MTR_6g007647 | 59.574 | 47 | 19 | 0 | 35 | 81 | 35 | 81 | 2.05e-13 | 67.8 |
| MsG0880042953.01.T01 | MTR_6g007647 | 59.574 | 47 | 19 | 0 | 35 | 81 | 69 | 115 | 3.17e-13 | 67.4 |
| MsG0880042953.01.T01 | MTR_7g103340 | 57.447 | 47 | 20 | 0 | 35 | 81 | 65 | 111 | 3.58e-13 | 66.6 |
| MsG0880042953.01.T01 | MTR_3g086790 | 42.553 | 94 | 43 | 2 | 13 | 99 | 44 | 133 | 6.72e-13 | 66.6 |
| MsG0880042953.01.T01 | MTR_4g107650 | 57.447 | 47 | 20 | 0 | 35 | 81 | 97 | 143 | 5.71e-12 | 64.3 |
| MsG0880042953.01.T01 | MTR_7g010020 | 54.348 | 46 | 21 | 0 | 36 | 81 | 83 | 128 | 2.39e-11 | 62.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880042953.01.T01 | AT2G46680 | 40.316 | 253 | 108 | 8 | 8 | 218 | 7 | 258 | 1.05e-39 | 137 |
| MsG0880042953.01.T01 | AT2G46680 | 39.841 | 251 | 110 | 8 | 8 | 218 | 7 | 256 | 2.63e-39 | 136 |
| MsG0880042953.01.T01 | AT3G61890 | 44.498 | 209 | 84 | 8 | 36 | 218 | 33 | 235 | 6.77e-38 | 132 |
| MsG0880042953.01.T01 | AT5G65310 | 38.636 | 132 | 61 | 4 | 20 | 140 | 47 | 169 | 1.46e-15 | 74.3 |
| MsG0880042953.01.T01 | AT5G65310 | 38.636 | 132 | 61 | 4 | 20 | 140 | 65 | 187 | 1.66e-15 | 74.3 |
| MsG0880042953.01.T01 | AT1G69780 | 45.545 | 101 | 40 | 4 | 15 | 108 | 72 | 164 | 2.77e-15 | 73.6 |
| MsG0880042953.01.T01 | AT5G15150 | 40.196 | 102 | 48 | 3 | 11 | 105 | 96 | 191 | 1.20e-14 | 71.6 |
| MsG0880042953.01.T01 | AT3G01470 | 32.812 | 128 | 63 | 2 | 35 | 139 | 70 | 197 | 4.73e-14 | 69.7 |
| MsG0880042953.01.T01 | AT4G40060 | 36.283 | 113 | 55 | 2 | 35 | 133 | 61 | 170 | 5.53e-14 | 69.7 |
| MsG0880042953.01.T01 | AT2G22430 | 61.702 | 47 | 18 | 0 | 35 | 81 | 64 | 110 | 2.00e-13 | 68.2 |
| MsG0880042953.01.T01 | AT3G01220 | 47.059 | 68 | 33 | 1 | 14 | 81 | 71 | 135 | 2.68e-13 | 67.8 |
| MsG0880042953.01.T01 | AT1G26960 | 50.725 | 69 | 28 | 2 | 13 | 81 | 57 | 119 | 7.79e-12 | 63.2 |
| MsG0880042953.01.T01 | AT5G03790 | 53.191 | 47 | 22 | 0 | 35 | 81 | 79 | 125 | 5.64e-11 | 60.5 |
| MsG0880042953.01.T01 | AT5G03790 | 53.191 | 47 | 22 | 0 | 35 | 81 | 79 | 125 | 7.05e-11 | 59.7 |
| MsG0880042953.01.T01 | AT2G18550 | 55.319 | 47 | 21 | 0 | 35 | 81 | 63 | 109 | 7.53e-11 | 60.1 |
Find 34 sgRNAs with CRISPR-Local
Find 55 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTACTCTGACTTTGCTCTAT+TGG | 0.221851 | 8:-16573754 | None:intergenic |
| GCTAATTGCAACTTCTTTCT+TGG | 0.222692 | 8:-16573192 | None:intergenic |
| ATTATCAATGGTGGGACTTT+TGG | 0.297638 | 8:+16574055 | MsG0880042953.01.T01:CDS |
| CTCTGCGGTTTGATCATGTT+TGG | 0.309960 | 8:+16574242 | MsG0880042953.01.T01:three_prime_UTR |
| TTTGAATCAAATGATTTGTT+AGG | 0.319546 | 8:+16574018 | MsG0880042953.01.T01:CDS |
| AGCCAAGACAAGTTGCTATA+TGG | 0.331941 | 8:+16573232 | MsG0880042953.01.T01:CDS |
| CAACATAATCATTAGTTTGT+AGG | 0.346723 | 8:+16574206 | MsG0880042953.01.T01:three_prime_UTR |
| TCAAAAGCACTCCAATCTTC+TGG | 0.368233 | 8:-16574000 | None:intergenic |
| ATGGAAAGTGCAGCAGAAAA+TGG | 0.377944 | 8:+16573802 | MsG0880042953.01.T01:CDS |
| TGTTCTGATCTTTCCATTGA+AGG | 0.384421 | 8:-16573862 | None:intergenic |
| CTTTAACATCACCAGAAGAT+TGG | 0.390865 | 8:+16573989 | MsG0880042953.01.T01:CDS |
| TGATGAACAAATCAAATCAT+TGG | 0.409448 | 8:+16573137 | MsG0880042953.01.T01:CDS |
| TTTGGTTTAGAAGATGAAAC+TGG | 0.409611 | 8:+16573934 | MsG0880042953.01.T01:CDS |
| AGCATAAAAGTTGAATACTT+TGG | 0.419671 | 8:+16573916 | MsG0880042953.01.T01:CDS |
| GATCTTTCCATTGAAGGACT+TGG | 0.420894 | 8:-16573856 | None:intergenic |
| TTTCAAAACAAAGAGCTAGA+TGG | 0.436326 | 8:+16573255 | MsG0880042953.01.T01:CDS |
| TTGCAATTAGCTAGAGAGCT+TGG | 0.453561 | 8:+16573204 | MsG0880042953.01.T01:CDS |
| AACCATATAGCAACTTGTCT+TGG | 0.480304 | 8:-16573234 | None:intergenic |
| GGTGAAACCAAGTCCTTCAA+TGG | 0.505148 | 8:+16573849 | MsG0880042953.01.T01:CDS |
| TATAGTTATTCAGCAGAAGC+AGG | 0.509566 | 8:+16573039 | MsG0880042953.01.T01:CDS |
| AATCAAGTTGTGATTATCAA+TGG | 0.520398 | 8:+16574043 | MsG0880042953.01.T01:CDS |
| AACAATCAAATGTGAGGCTG+AGG | 0.535331 | 8:+16573828 | MsG0880042953.01.T01:CDS |
| CAAGTTGTGATTATCAATGG+TGG | 0.557189 | 8:+16574046 | MsG0880042953.01.T01:CDS |
| ACAGAGGAGGCAAAATTATG+TGG | 0.582377 | 8:+16574089 | MsG0880042953.01.T01:three_prime_UTR |
| AAGTTGTCAGGGGAAAATCA+AGG | 0.603486 | 8:+16574164 | MsG0880042953.01.T01:three_prime_UTR |
| AAGTTGTGATTATCAATGGT+GGG | 0.604345 | 8:+16574047 | MsG0880042953.01.T01:CDS |
| AGTTGTCAGGGGAAAATCAA+GGG | 0.618503 | 8:+16574165 | MsG0880042953.01.T01:three_prime_UTR |
| GGTCTTGAATGAAACAGAGG+AGG | 0.619886 | 8:+16574076 | MsG0880042953.01.T01:exon |
| TTTGGTCTTGAATGAAACAG+AGG | 0.622140 | 8:+16574073 | MsG0880042953.01.T01:exon |
| AGTTAAAGAAGCAAACAGCA+TGG | 0.635070 | 8:+16573783 | MsG0880042953.01.T01:CDS |
| TGGAGGAACAATCAAATGTG+AGG | 0.637358 | 8:+16573822 | MsG0880042953.01.T01:CDS |
| TTTGTTGAAGAAGTTGTCAG+GGG | 0.642443 | 8:+16574154 | MsG0880042953.01.T01:three_prime_UTR |
| GAGGAGGCAAAATTATGTGG+AGG | 0.659550 | 8:+16574092 | MsG0880042953.01.T01:three_prime_UTR |
| GAAAGTGCAGCAGAAAATGG+AGG | 0.672573 | 8:+16573805 | MsG0880042953.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATTAAACATTAAAATTGAAT+TGG | - | Chr8:16573466-16573485 | None:intergenic | 10.0% |
| !! | TCAAAATAGTTACAATAATT+TGG | + | Chr8:16573313-16573332 | MsG0880042953.01.T01:intron | 15.0% |
| !! | TGCGAAATTATTATTAATAA+CGG | + | Chr8:16573513-16573532 | MsG0880042953.01.T01:intron | 15.0% |
| !! | GAAATTATTATTAATAACGG+TGG | + | Chr8:16573516-16573535 | MsG0880042953.01.T01:intron | 20.0% |
| !! | TAATCATATAGTAATTTGAC+TGG | + | Chr8:16573687-16573706 | MsG0880042953.01.T01:intron | 20.0% |
| !!! | ATGAATTTTTTTGCTTAGAA+TGG | + | Chr8:16573421-16573440 | MsG0880042953.01.T01:intron | 20.0% |
| !!! | TTTGAATCAAATGATTTGTT+AGG | + | Chr8:16574018-16574037 | MsG0880042953.01.T01:CDS | 20.0% |
| ! | AAAGTTTGAATCTATGAAGA+AGG | + | Chr8:16573340-16573359 | MsG0880042953.01.T01:intron | 25.0% |
| ! | AAGACAAACATTACTAATAC+AGG | + | Chr8:16573364-16573383 | MsG0880042953.01.T01:intron | 25.0% |
| ! | AGAAAAACAAGAATACAAAG+AGG | + | Chr8:16573109-16573128 | MsG0880042953.01.T01:CDS | 25.0% |
| ! | AGCATAAAAGTTGAATACTT+TGG | + | Chr8:16573916-16573935 | MsG0880042953.01.T01:CDS | 25.0% |
| ! | ATCTTGTCAACATTTCAAAA+TGG | - | Chr8:16573489-16573508 | None:intergenic | 25.0% |
| ! | TGATGAACAAATCAAATCAT+TGG | + | Chr8:16573137-16573156 | MsG0880042953.01.T01:CDS | 25.0% |
| !! | AATCAAGTTGTGATTATCAA+TGG | + | Chr8:16574043-16574062 | MsG0880042953.01.T01:CDS | 25.0% |
| !!! | CAACATAATCATTAGTTTGT+AGG | + | Chr8:16574206-16574225 | MsG0880042953.01.T01:three_prime_UTR | 25.0% |
| AAGTTGTGATTATCAATGGT+GGG | + | Chr8:16574047-16574066 | MsG0880042953.01.T01:CDS | 30.0% | |
| TTTCAAAACAAAGAGCTAGA+TGG | + | Chr8:16573255-16573274 | MsG0880042953.01.T01:CDS | 30.0% | |
| ! | TTTGGTTTAGAAGATGAAAC+TGG | + | Chr8:16573934-16573953 | MsG0880042953.01.T01:CDS | 30.0% |
| ! | TTTTGTTGAAGAAGTTGTCA+GGG | + | Chr8:16574153-16574172 | MsG0880042953.01.T01:three_prime_UTR | 30.0% |
| !! | CTTGTTTTTCTTCTTTCTCA+TGG | - | Chr8:16573100-16573119 | None:intergenic | 30.0% |
| AACCATATAGCAACTTGTCT+TGG | - | Chr8:16573237-16573256 | None:intergenic | 35.0% | |
| AGTTAAAGAAGCAAACAGCA+TGG | + | Chr8:16573783-16573802 | MsG0880042953.01.T01:CDS | 35.0% | |
| CAAGTTGTGATTATCAATGG+TGG | + | Chr8:16574046-16574065 | MsG0880042953.01.T01:CDS | 35.0% | |
| CTAATAACCATCGTGTTCAT+CGG | - | Chr8:16573615-16573634 | None:intergenic | 35.0% | |
| CTTTAACATCACCAGAAGAT+TGG | + | Chr8:16573989-16574008 | MsG0880042953.01.T01:CDS | 35.0% | |
| GATGAACACGATGGTTATTA+GGG | + | Chr8:16573614-16573633 | MsG0880042953.01.T01:intron | 35.0% | |
| GCTAATTGCAACTTCTTTCT+TGG | - | Chr8:16573195-16573214 | None:intergenic | 35.0% | |
| TATAGTTATTCAGCAGAAGC+AGG | + | Chr8:16573039-16573058 | MsG0880042953.01.T01:CDS | 35.0% | |
| TGTTCTGATCTTTCCATTGA+AGG | - | Chr8:16573865-16573884 | None:intergenic | 35.0% | |
| TTTGTTGAAGAAGTTGTCAG+GGG | + | Chr8:16574154-16574173 | MsG0880042953.01.T01:three_prime_UTR | 35.0% | |
| ! | AATTTTGCTGAACATGCTGA+TGG | + | Chr8:16573964-16573983 | MsG0880042953.01.T01:CDS | 35.0% |
| ! | GATTGACTTTGTATCTTCAG+TGG | - | Chr8:16573573-16573592 | None:intergenic | 35.0% |
| ! | GGAAATGATTTTCATCTCTG+CGG | + | Chr8:16574227-16574246 | MsG0880042953.01.T01:three_prime_UTR | 35.0% |
| !! | ATTATCAATGGTGGGACTTT+TGG | + | Chr8:16574055-16574074 | MsG0880042953.01.T01:CDS | 35.0% |
| !! | GTTTTGTTGAAGAAGTTGTC+AGG | + | Chr8:16574152-16574171 | MsG0880042953.01.T01:three_prime_UTR | 35.0% |
| !! | TTTGGTCTTGAATGAAACAG+AGG | + | Chr8:16574073-16574092 | MsG0880042953.01.T01:exon | 35.0% |
| AACAATCAAATGTGAGGCTG+AGG | + | Chr8:16573828-16573847 | MsG0880042953.01.T01:CDS | 40.0% | |
| AAGTTGTCAGGGGAAAATCA+AGG | + | Chr8:16574164-16574183 | MsG0880042953.01.T01:three_prime_UTR | 40.0% | |
| ACAGAGGAGGCAAAATTATG+TGG | + | Chr8:16574089-16574108 | MsG0880042953.01.T01:three_prime_UTR | 40.0% | |
| AGCCAAGACAAGTTGCTATA+TGG | + | Chr8:16573232-16573251 | MsG0880042953.01.T01:CDS | 40.0% | |
| AGTTGTCAGGGGAAAATCAA+GGG | + | Chr8:16574165-16574184 | MsG0880042953.01.T01:three_prime_UTR | 40.0% | |
| CGATGAACACGATGGTTATT+AGG | + | Chr8:16573613-16573632 | MsG0880042953.01.T01:intron | 40.0% | |
| CTACTCTGACTTTGCTCTAT+TGG | - | Chr8:16573757-16573776 | None:intergenic | 40.0% | |
| GATCTTTCCATTGAAGGACT+TGG | - | Chr8:16573859-16573878 | None:intergenic | 40.0% | |
| TGGAGGAACAATCAAATGTG+AGG | + | Chr8:16573822-16573841 | MsG0880042953.01.T01:CDS | 40.0% | |
| TTGCAATTAGCTAGAGAGCT+TGG | + | Chr8:16573204-16573223 | MsG0880042953.01.T01:CDS | 40.0% | |
| ! | ATGGAAAGTGCAGCAGAAAA+TGG | + | Chr8:16573802-16573821 | MsG0880042953.01.T01:CDS | 40.0% |
| !! | TCAAAAGCACTCCAATCTTC+TGG | - | Chr8:16574003-16574022 | None:intergenic | 40.0% |
| CTCTGCGGTTTGATCATGTT+TGG | + | Chr8:16574242-16574261 | MsG0880042953.01.T01:three_prime_UTR | 45.0% | |
| GAGGAGGCAAAATTATGTGG+AGG | + | Chr8:16574092-16574111 | MsG0880042953.01.T01:three_prime_UTR | 45.0% | |
| GGTCTTGAATGAAACAGAGG+AGG | + | Chr8:16574076-16574095 | MsG0880042953.01.T01:exon | 45.0% | |
| GGTGAAACCAAGTCCTTCAA+TGG | + | Chr8:16573849-16573868 | MsG0880042953.01.T01:CDS | 45.0% | |
| GTTCATCGGTATCAACTCAG+TGG | - | Chr8:16573601-16573620 | None:intergenic | 45.0% | |
| GTTGATACCGATGAACACGA+TGG | + | Chr8:16573605-16573624 | MsG0880042953.01.T01:intron | 45.0% | |
| ! | GAAAGTGCAGCAGAAAATGG+AGG | + | Chr8:16573805-16573824 | MsG0880042953.01.T01:CDS | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr8 | gene | 16573024 | 16574334 | 16573024 | ID=MsG0880042953.01;Name=MsG0880042953.01 |
| Chr8 | mRNA | 16573024 | 16574334 | 16573024 | ID=MsG0880042953.01.T01;Parent=MsG0880042953.01;Name=MsG0880042953.01.T01;_AED=0.50;_eAED=0.50;_QI=0|0|0.5|1|1|1|2|251|218 |
| Chr8 | exon | 16573024 | 16573320 | 16573024 | ID=MsG0880042953.01.T01:exon:2317;Parent=MsG0880042953.01.T01 |
| Chr8 | exon | 16573724 | 16574334 | 16573724 | ID=MsG0880042953.01.T01:exon:2318;Parent=MsG0880042953.01.T01 |
| Chr8 | CDS | 16573024 | 16573320 | 16573024 | ID=MsG0880042953.01.T01:cds;Parent=MsG0880042953.01.T01 |
| Chr8 | CDS | 16573724 | 16574083 | 16573724 | ID=MsG0880042953.01.T01:cds;Parent=MsG0880042953.01.T01 |
| Chr8 | three_prime_UTR | 16574084 | 16574334 | 16574084 | ID=MsG0880042953.01.T01:three_prime_utr;Parent=MsG0880042953.01.T01 |
| Gene Sequence |
| Protein sequence |
