AlfalfaGEDB | MsG0880042973.01

M. sativa cultivar ZhongmuNo.1 / MsG0880042973.01

KEGG: -
GO: GO:0003677 GO:0006355
PFAM: PF02701

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880042973.01.T01	XP_024629469.1	96.774	93	3	0	1	93	1	93	9.73E-59	191

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880042973.01.T01	Q43385	83.562	73	12	0	16	88	66	138	6.61E-40	136

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880042973.01.T01	A0A072TM78	96.774	93	3	0	1	93	1	93	4.65e-59	191

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0180000555.01	MsG0880042973.01	0.800804	1.213618e-48	5.703535e-46
MsG0180000585.01	MsG0880042973.01	0.804511	2.086149e-49	1.077584e-46
MsG0180000741.01	MsG0880042973.01	0.800014	1.757614e-48	8.096214e-46
MsG0180001117.01	MsG0880042973.01	0.801640	8.183871e-49	3.928237e-46
MsG0180001178.01	MsG0880042973.01	0.824379	8.334210e-54	7.369479e-51
MsG0180001565.01	MsG0880042973.01	0.849354	3.413419e-60	6.454000e-57
MsG0180001566.01	MsG0880042973.01	0.809205	2.123304e-50	1.238980e-47
MsG0180004139.01	MsG0880042973.01	0.825930	3.582699e-54	3.311828e-51
MsG0180004147.01	MsG0880042973.01	0.803269	3.778659e-49	1.890610e-46
MsG0180004320.01	MsG0880042973.01	0.800978	1.118367e-48	5.279587e-46
MsG0180005559.01	MsG0880042973.01	0.830110	3.530952e-55	3.684972e-52
MsG0180005846.01	MsG0880042973.01	0.822695	2.064658e-53	1.740822e-50
MsG0180006260.01	MsG0880042973.01	0.802301	5.988047e-49	2.923131e-46
MsG0280006425.01	MsG0880042973.01	0.808677	2.753504e-50	1.584192e-47
MsG0780040290.01	MsG0880042973.01	0.835124	2.013755e-56	2.439253e-53
MsG0780040488.01	MsG0880042973.01	0.840974	6.291033e-58	9.111397e-55
MsG0780040541.01	MsG0880042973.01	0.809407	1.921223e-50	1.127122e-47
MsG0780041310.01	MsG0880042973.01	0.805585	1.243432e-49	6.603321e-47
MsG0880042973.01	MsG0880043299.01	0.801538	8.587713e-49	4.111554e-46
MsG0880042973.01	MsG0880043921.01	0.853589	2.162204e-61	4.695889e-58
MsG0880042973.01	MsG0880045449.01	0.816286	5.979571e-52	4.219769e-49
MsG0880042973.01	MsG0880045786.01	0.820467	6.755793e-53	5.349890e-50
MsG0880042973.01	MsG0880047293.01	0.811314	7.447812e-51	4.594918e-48
MsG0880042973.01	MsG0880047337.01	0.801005	1.104404e-48	5.217278e-46
MsG0280010614.01	MsG0880042973.01	0.824076	9.816395e-54	8.605802e-51
MsG0280010868.01	MsG0880042973.01	0.825678	4.111402e-54	3.773222e-51
MsG0280011085.01	MsG0880042973.01	0.808026	3.791385e-50	2.144398e-47
MsG0280011285.01	MsG0880042973.01	0.814783	1.292332e-51	8.752273e-49
MsG0280011293.01	MsG0880042973.01	0.813580	2.382014e-51	1.561574e-48
MsG0480019502.01	MsG0880042973.01	0.808241	3.411371e-50	1.940560e-47
MsG0480022719.01	MsG0880042973.01	0.803769	2.976486e-49	1.508526e-46
MsG0480023421.01	MsG0880042973.01	0.813784	2.147982e-51	1.415919e-48
MsG0580024157.01	MsG0880042973.01	0.829052	6.384951e-55	6.460458e-52
MsG0380015495.01	MsG0880042973.01	0.809419	1.910266e-50	1.121049e-47
MsG0380017946.01	MsG0880042973.01	0.831755	1.394299e-55	1.527537e-52
MsG0580027208.01	MsG0880042973.01	0.822422	2.389511e-53	1.999222e-50
MsG0580028307.01	MsG0880042973.01	0.819254	1.279220e-52	9.793711e-50
MsG0680030436.01	MsG0880042973.01	0.810862	9.333084e-51	5.690120e-48
MsG0280007740.01	MsG0880042973.01	0.803668	3.123334e-49	1.578800e-46
MsG0280010173.01	MsG0880042973.01	0.826301	2.925225e-54	2.732557e-51
MsG0280010239.01	MsG0880042973.01	0.807629	4.604065e-50	2.577142e-47
MsG0280010459.01	MsG0880042973.01	0.821003	5.085727e-53	4.088318e-50
MsG0780037975.01	MsG0880042973.01	0.800004	1.765920e-48	8.132458e-46
MsG0780038837.01	MsG0880042973.01	0.808352	3.230651e-50	1.843113e-47
MsG0780039249.01	MsG0880042973.01	0.808373	3.196944e-50	1.824894e-47
MsG0780039654.01	MsG0880042973.01	0.821093	4.850483e-53	3.908583e-50
MsG0780039655.01	MsG0880042973.01	0.801420	9.081098e-49	4.334789e-46

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880042973.01.T01	MTR_8g027295	96.774	93	3	0	1	93	1	93	1.18e-62	191
MsG0880042973.01.T01	MTR_3g091820	79.012	81	14	2	1	78	17	97	3.26e-39	132
MsG0880042973.01.T01	MTR_1g077600	73.171	82	18	2	1	78	16	97	1.69e-37	127
MsG0880042973.01.T01	MTR_1g077600	73.171	82	18	2	1	78	16	97	1.85e-37	127
MsG0880042973.01.T01	MTR_2g093220	75.000	72	16	1	9	78	23	94	4.11e-34	119
MsG0880042973.01.T01	MTR_8g479350	75.806	62	15	0	21	82	65	126	2.77e-31	112
MsG0880042973.01.T01	MTR_8g068210	65.060	83	26	2	15	95	21	102	3.39e-31	112
MsG0880042973.01.T01	MTR_4g063780	72.059	68	19	0	11	78	62	129	1.55e-30	110
MsG0880042973.01.T01	MTR_3g090430	76.562	64	13	1	17	78	42	105	4.30e-30	109
MsG0880042973.01.T01	MTR_4g461080	69.014	71	20	1	8	76	11	81	4.80e-30	107
MsG0880042973.01.T01	MTR_4g022370	76.271	59	14	0	20	78	78	136	4.84e-30	109
MsG0880042973.01.T01	MTR_1g056810	87.037	54	7	0	25	78	13	66	7.68e-30	107
MsG0880042973.01.T01	MTR_4g088580	71.429	70	16	1	26	95	57	122	1.03e-29	108
MsG0880042973.01.T01	MTR_3g077750	68.657	67	19	1	10	74	45	111	2.29e-29	107
MsG0880042973.01.T01	MTR_8g079060	72.131	61	17	0	18	78	23	83	2.58e-29	104
MsG0880042973.01.T01	MTR_2g096740	80.000	55	11	0	24	78	77	131	6.69e-29	105
MsG0880042973.01.T01	MTR_2g096740	75.862	58	14	0	21	78	59	116	6.87e-29	105
MsG0880042973.01.T01	MTR_2g014060	77.966	59	12	1	17	75	19	76	1.04e-28	105
MsG0880042973.01.T01	MTR_5g031440	78.182	55	12	0	20	74	39	93	1.22e-28	105
MsG0880042973.01.T01	MTR_8g015840	74.138	58	15	0	21	78	17	74	1.25e-28	102
MsG0880042973.01.T01	MTR_4g089095	74.138	58	14	1	22	79	48	104	6.71e-28	102
MsG0880042973.01.T01	MTR_2g013370	58.108	74	30	1	6	79	28	100	6.94e-28	102
MsG0880042973.01.T01	MTR_7g024670	83.019	53	9	0	26	78	79	131	7.37e-28	103
MsG0880042973.01.T01	MTR_4g089095	74.138	58	14	1	22	79	14	70	7.97e-28	102
MsG0880042973.01.T01	MTR_7g059400	79.245	53	11	0	26	78	78	130	1.31e-27	102
MsG0880042973.01.T01	MTR_3g435480	61.333	75	20	1	19	93	131	196	1.86e-27	103
MsG0880042973.01.T01	MTR_4g082060	64.062	64	23	0	19	82	117	180	1.38e-26	101
MsG0880042973.01.T01	MTR_7g086780	61.905	63	24	0	19	81	94	156	1.83e-26	100
MsG0880042973.01.T01	MTR_7g010950	58.974	78	25	1	19	89	130	207	2.05e-26	101
MsG0880042973.01.T01	MTR_2g059540	81.633	49	9	0	24	72	31	79	2.50e-26	96.7
MsG0880042973.01.T01	MTR_4g109980	77.358	53	12	0	22	74	38	90	3.19e-26	99.0
MsG0880042973.01.T01	MTR_6g027450	60.317	63	25	0	19	81	84	146	6.18e-26	98.2
MsG0880042973.01.T01	MTR_2g014170	79.592	49	10	0	24	72	45	93	1.25e-25	97.1
MsG0880042973.01.T01	MTR_8g044220	61.290	62	24	0	19	80	113	174	1.69e-25	98.2
MsG0880042973.01.T01	MTR_6g027460	60.317	63	25	0	19	81	91	153	1.86e-25	97.4
MsG0880042973.01.T01	MTR_2g016030	63.636	66	22	1	12	77	37	100	1.93e-25	93.2
MsG0880042973.01.T01	MTR_6g012450	66.102	59	20	0	19	77	131	189	3.68e-25	97.8
MsG0880042973.01.T01	MTR_5g041420	62.712	59	22	0	19	77	133	191	2.73e-24	93.6
MsG0880042973.01.T01	MTR_5g041530	62.500	64	23	1	15	77	130	193	1.95e-22	89.4
MsG0880042973.01.T01	MTR_5g041400	50.000	86	42	1	11	95	106	191	4.87e-22	88.2
MsG0880042973.01.T01	MTR_5g041380	53.731	67	31	0	11	77	114	180	7.58e-22	87.8

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880042973.01.T01	AT3G61850	83.562	73	12	0	16	88	54	126	5.13e-41	136
MsG0880042973.01.T01	AT3G61850	83.562	73	12	0	16	88	54	126	5.13e-41	136
MsG0880042973.01.T01	AT3G61850	83.562	73	12	0	16	88	66	138	6.74e-41	136
MsG0880042973.01.T01	AT3G61850	83.562	73	12	0	16	88	54	126	9.01e-41	136
MsG0880042973.01.T01	AT3G61850	83.562	73	12	0	16	88	66	138	9.89e-41	136
MsG0880042973.01.T01	AT4G24060	83.824	68	9	1	13	78	40	107	8.98e-37	127
MsG0880042973.01.T01	AT4G00940	87.302	63	8	0	18	80	62	124	4.59e-36	124
MsG0880042973.01.T01	AT4G00940	87.302	63	8	0	18	80	62	124	4.59e-36	124
MsG0880042973.01.T01	AT4G00940	87.302	63	8	0	18	80	62	124	4.59e-36	124
MsG0880042973.01.T01	AT2G46590	92.982	57	4	0	15	71	59	115	8.99e-36	124
MsG0880042973.01.T01	AT2G46590	92.982	57	4	0	15	71	71	127	1.18e-35	124
MsG0880042973.01.T01	AT5G60200	68.000	75	24	0	10	84	41	115	4.99e-33	115
MsG0880042973.01.T01	AT3G55370	73.529	68	18	0	11	78	63	130	2.70e-32	114
MsG0880042973.01.T01	AT3G55370	73.529	68	18	0	11	78	108	175	2.79e-32	115
MsG0880042973.01.T01	AT3G55370	73.529	68	18	0	11	78	63	130	3.56e-32	115
MsG0880042973.01.T01	AT1G64620	76.923	65	13	1	10	72	33	97	1.88e-31	113
MsG0880042973.01.T01	AT1G51700	75.862	58	14	0	21	78	30	87	8.93e-31	107
MsG0880042973.01.T01	AT3G45610	69.231	65	20	0	18	82	34	98	1.37e-30	108
MsG0880042973.01.T01	AT2G37590	75.410	61	15	0	18	78	83	143	2.29e-30	109
MsG0880042973.01.T01	AT5G65590	75.410	61	15	0	18	78	35	95	5.84e-30	108
MsG0880042973.01.T01	AT5G66940	74.138	58	15	0	21	78	29	86	9.78e-30	105
MsG0880042973.01.T01	AT3G50410	74.138	58	15	0	21	78	27	84	1.25e-29	106
MsG0880042973.01.T01	AT2G28510	78.689	61	13	0	22	82	46	106	1.48e-29	107
MsG0880042973.01.T01	AT3G52440	82.456	57	10	0	26	82	27	83	4.48e-29	104
MsG0880042973.01.T01	AT3G52440	82.456	57	10	0	26	82	46	102	4.81e-29	105
MsG0880042973.01.T01	AT3G21270	81.132	53	10	0	24	76	29	81	8.86e-29	102
MsG0880042973.01.T01	AT5G02460	67.647	68	22	0	11	78	82	149	1.54e-28	106
MsG0880042973.01.T01	AT1G07640	78.182	55	12	0	24	78	21	75	2.13e-28	103
MsG0880042973.01.T01	AT1G07640	78.182	55	12	0	24	78	77	131	2.50e-28	104
MsG0880042973.01.T01	AT1G07640	78.182	55	12	0	24	78	85	139	3.03e-28	104
MsG0880042973.01.T01	AT1G28310	75.000	56	14	0	24	79	27	82	4.05e-28	103
MsG0880042973.01.T01	AT5G60850	68.657	67	16	1	21	82	48	114	4.34e-28	103
MsG0880042973.01.T01	AT4G38000	64.474	76	24	2	10	83	25	99	4.58e-28	102
MsG0880042973.01.T01	AT1G28310	75.000	56	14	0	24	79	51	106	5.89e-28	103
MsG0880042973.01.T01	AT2G28810	65.217	69	23	1	8	75	60	128	6.06e-28	103
MsG0880042973.01.T01	AT2G28810	65.217	69	23	1	8	75	77	145	8.23e-28	103
MsG0880042973.01.T01	AT1G21340	71.212	66	16	1	16	78	27	92	9.15e-28	101
MsG0880042973.01.T01	AT1G69570	70.312	64	19	0	19	82	127	190	9.34e-28	103
MsG0880042973.01.T01	AT2G34140	58.108	74	31	0	4	77	38	111	2.44e-27	98.2
MsG0880042973.01.T01	AT1G29160	61.765	68	26	0	10	77	48	115	3.75e-27	97.8
MsG0880042973.01.T01	AT5G62940	85.714	49	7	0	26	74	75	123	4.98e-27	101
MsG0880042973.01.T01	AT4G21050	74.074	54	14	0	26	79	26	79	2.08e-26	97.1
MsG0880042973.01.T01	AT1G47655	76.923	52	12	0	22	73	27	78	3.44e-26	96.3
MsG0880042973.01.T01	AT5G39660	62.857	70	24	1	8	77	124	191	5.12e-26	99.8
MsG0880042973.01.T01	AT5G39660	62.857	70	24	1	8	77	124	191	5.12e-26	99.8
MsG0880042973.01.T01	AT5G39660	62.857	70	24	1	8	77	124	191	5.12e-26	99.8
MsG0880042973.01.T01	AT3G47500	66.102	59	20	0	19	77	105	163	3.64e-25	97.4
MsG0880042973.01.T01	AT5G62430	64.407	59	21	0	19	77	49	107	4.49e-25	95.5
MsG0880042973.01.T01	AT1G26790	64.062	64	23	0	19	82	106	169	5.94e-25	96.3
MsG0880042973.01.T01	AT4G21040	79.592	49	10	0	26	74	27	75	7.81e-25	93.6
MsG0880042973.01.T01	AT4G21080	75.510	49	12	0	26	74	27	75	1.80e-23	90.1
MsG0880042973.01.T01	AT4G21030	60.870	46	18	0	26	71	23	68	1.44e-13	63.2

Find 21 sgRNAs with CRISPR-Local

Find 20 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CCTTGCCTTGGACATGAATT+AGG	0.219906	8:+16868638	None:intergenic
TTCTTCTCTAATCCTTGCCT+TGG	0.373577	8:+16868626	None:intergenic
CATGTCTTGCAAAAGTATCT+TGG	0.386535	8:+16868515	None:intergenic
GCAAGACATGTAGAAGGTAT+TGG	0.389030	8:-16868502	MsG0880042973.01.T01:CDS
GGACAATTTATTTGCTCTTG+TGG	0.430301	8:+16868596	None:intergenic
TTTCTTGAACCACCTCCAAC+AGG	0.434621	8:+16868452	None:intergenic
AGGGTAGGTGGATTTAGGTC+AGG	0.459379	8:+16868375	None:intergenic
CTAAGAAATGTTCCTGTTGG+AGG	0.487652	8:-16868464	MsG0880042973.01.T01:CDS
TCTCTAAGAAATGTTCCTGT+TGG	0.507663	8:-16868467	MsG0880042973.01.T01:CDS
AGAAATGTTCCTGTTGGAGG+TGG	0.521904	8:-16868461	MsG0880042973.01.T01:CDS
TGATGAACTATTATTAGGTG+TGG	0.533433	8:+16868403	None:intergenic
TGTAGAAGGTATTGGACTGA+AGG	0.537478	8:-16868494	MsG0880042973.01.T01:CDS
TTAGGTGTGGCTAATGATGA+AGG	0.550310	8:+16868416	None:intergenic
TAGGTATATATACTCATGGA+AGG	0.557450	8:-16868668	None:intergenic
AAGAGCAAATAAATTGTCCA+AGG	0.610950	8:-16868592	MsG0880042973.01.T01:CDS
TAGCTCCTAATTCATGTCCA+AGG	0.613211	8:-16868643	MsG0880042973.01.T01:CDS
CCTAATTCATGTCCAAGGCA+AGG	0.615681	8:-16868638	MsG0880042973.01.T01:CDS
AGAAGGTATTGGACTGAAGG+AGG	0.618740	8:-16868491	MsG0880042973.01.T01:CDS
AAGGATTAGAGAAGAAAGCA+AGG	0.621824	8:-16868619	MsG0880042973.01.T01:CDS
GTGTTTGTTGAATTGCACCT+TGG	0.631036	8:+16868575	None:intergenic
AGGTATATATACTCATGGAA+GGG	0.656170	8:-16868667	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	AATTTTGATGAACTATTATT+AGG	+	Chr8:16868646-16868665	None:intergenic	15.0%
	AAGAGCAAATAAATTGTCCA+AGG	-	Chr8:16868449-16868468	MsG0880042973.01.T01:CDS	30.0%
	TGATGAACTATTATTAGGTG+TGG	+	Chr8:16868641-16868660	None:intergenic	30.0%
	AAGGATTAGAGAAGAAAGCA+AGG	-	Chr8:16868422-16868441	MsG0880042973.01.T01:CDS	35.0%
	GGACAATTTATTTGCTCTTG+TGG	+	Chr8:16868448-16868467	None:intergenic	35.0%
!	ACTTTTGCAAGACATGTAGA+AGG	-	Chr8:16868533-16868552	MsG0880042973.01.T01:CDS	35.0%
!	TCTCTAAGAAATGTTCCTGT+TGG	-	Chr8:16868574-16868593	MsG0880042973.01.T01:CDS	35.0%
!!	CATGTCTTGCAAAAGTATCT+TGG	+	Chr8:16868529-16868548	None:intergenic	35.0%
	CTAAGAAATGTTCCTGTTGG+AGG	-	Chr8:16868577-16868596	MsG0880042973.01.T01:CDS	40.0%
	TAGCTCCTAATTCATGTCCA+AGG	-	Chr8:16868398-16868417	MsG0880042973.01.T01:CDS	40.0%
	TTAGGTGTGGCTAATGATGA+AGG	+	Chr8:16868628-16868647	None:intergenic	40.0%
	TTCTTCTCTAATCCTTGCCT+TGG	+	Chr8:16868418-16868437	None:intergenic	40.0%
!	GCAAGACATGTAGAAGGTAT+TGG	-	Chr8:16868539-16868558	MsG0880042973.01.T01:CDS	40.0%
!	GTGTTTGTTGAATTGCACCT+TGG	+	Chr8:16868469-16868488	None:intergenic	40.0%
!!	TGTAGAAGGTATTGGACTGA+AGG	-	Chr8:16868547-16868566	MsG0880042973.01.T01:CDS	40.0%
	AGAAATGTTCCTGTTGGAGG+TGG	-	Chr8:16868580-16868599	MsG0880042973.01.T01:CDS	45.0%
	CCTAATTCATGTCCAAGGCA+AGG	-	Chr8:16868403-16868422	MsG0880042973.01.T01:CDS	45.0%
	CCTTGCCTTGGACATGAATT+AGG	+	Chr8:16868406-16868425	None:intergenic	45.0%
	TTTCTTGAACCACCTCCAAC+AGG	+	Chr8:16868592-16868611	None:intergenic	45.0%
!!	AGAAGGTATTGGACTGAAGG+AGG	-	Chr8:16868550-16868569	MsG0880042973.01.T01:CDS	45.0%

Chromosome	Type	Strat	End	Strand	Name
Chr8	gene	16868388	16868675	16868388	ID=MsG0880042973.01;Name=MsG0880042973.01
Chr8	mRNA	16868388	16868675	16868388	ID=MsG0880042973.01.T01;Parent=MsG0880042973.01;Name=MsG0880042973.01.T01;_AED=0.43;_eAED=0.43;_QI=0\|-1\|0\|1\|-1\|0\|1\|0\|95
Chr8	exon	16868388	16868675	16868388	ID=MsG0880042973.01.T01:exon:3257;Parent=MsG0880042973.01.T01
Chr8	CDS	16868388	16868675	16868388	ID=MsG0880042973.01.T01:cds;Parent=MsG0880042973.01.T01

Gene Sequence	>MsG0880042973.01.T01 ATGGAAGGGTTAGCTCCTAATTCATGTCCAAGGCAAGGATTAGAGAAGAAAGCAAGGCCACAAGAGCAAATAAATTGTCCAAGGTGCAATTCAACAAACACAAAATTTTGTTATTACAACAACTACAGTCTCACACAACCAAGATACTTTTGCAAGACATGTAGAAGGTATTGGACTGAAGGAGGATCTCTAAGAAATGTTCCTGTTGGAGGTGGTTCAAGAAAGAACAAGAAAATTCCTTCATCATTAGCCACACCTAATAATAGTTCATCAAAATTCCTGACCTAA
Protein sequence	>MsG0880042973.01.T01 MEGLAPNSCPRQGLEKKARPQEQINCPRCNSTNTKFCYYNNYSLTQPRYFCKTCRRYWTEGGSLRNVPVGGGSRKNKKIPSSLATPNNSSSKFLT*