Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880043428.01.T02 | XP_003627647.1 | 95.679 | 162 | 7 | 0 | 1 | 162 | 168 | 329 | 7.15E-109 | 323 |
| MsG0880043428.01.T01 | XP_003627647.1 | 95.614 | 228 | 10 | 0 | 1 | 228 | 102 | 329 | 2.01E-160 | 457 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880043428.01.T01 | O64640 | 54.31 | 232 | 102 | 2 | 1 | 228 | 89 | 320 | 2.52E-87 | 264 |
| MsG0880043428.01.T02 | O64640 | 57.669 | 163 | 68 | 1 | 1 | 162 | 158 | 320 | 2.10E-60 | 192 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880043428.01.T01 | G7LCQ6 | 95.614 | 228 | 10 | 0 | 1 | 228 | 102 | 329 | 9.59e-161 | 457 |
| MsG0880043428.01.T02 | G7LCQ6 | 95.679 | 162 | 7 | 0 | 1 | 162 | 168 | 329 | 3.41e-109 | 323 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|---|---|
| MsG0880043428.01 | MsG0180005648.01 | PPI |
| MsG0880043428.01 | MsG0780041226.01 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880043428.01.T02 | MTR_8g035520 | 95.679 | 162 | 7 | 0 | 1 | 162 | 168 | 329 | 8.66e-113 | 323 |
| MsG0880043428.01.T02 | MTR_8g035540 | 77.500 | 160 | 36 | 0 | 1 | 160 | 160 | 319 | 4.20e-87 | 258 |
| MsG0880043428.01.T02 | MTR_6g464510 | 56.790 | 162 | 66 | 3 | 1 | 158 | 166 | 327 | 4.52e-57 | 181 |
| MsG0880043428.01.T02 | MTR_6g464280 | 56.707 | 164 | 64 | 3 | 1 | 158 | 163 | 325 | 2.12e-54 | 174 |
| MsG0880043428.01.T02 | MTR_6g464320 | 52.695 | 167 | 72 | 3 | 1 | 161 | 164 | 329 | 3.69e-51 | 166 |
| MsG0880043428.01.T02 | MTR_8g035500 | 48.077 | 156 | 80 | 1 | 2 | 157 | 163 | 317 | 1.22e-47 | 157 |
| MsG0880043428.01.T02 | MTR_0007s0480 | 49.405 | 168 | 76 | 3 | 1 | 160 | 7 | 173 | 4.54e-46 | 148 |
| MsG0880043428.01.T02 | MTR_0007s0520 | 50.299 | 167 | 74 | 3 | 1 | 159 | 161 | 326 | 2.28e-44 | 149 |
| MsG0880043428.01.T02 | MTR_1g102170 | 47.826 | 161 | 74 | 3 | 1 | 154 | 178 | 335 | 2.39e-38 | 133 |
| MsG0880043428.01.T02 | MTR_1g102160 | 44.311 | 167 | 85 | 4 | 1 | 159 | 161 | 327 | 3.31e-38 | 133 |
| MsG0880043428.01.T02 | MTR_6g464120 | 46.711 | 152 | 78 | 3 | 1 | 149 | 166 | 317 | 5.05e-34 | 125 |
| MsG0880043428.01.T02 | MTR_6g065370 | 34.969 | 163 | 98 | 4 | 2 | 160 | 178 | 336 | 1.74e-21 | 89.0 |
| MsG0880043428.01.T02 | MTR_6g065400 | 34.969 | 163 | 98 | 4 | 2 | 160 | 178 | 336 | 2.32e-21 | 89.0 |
| MsG0880043428.01.T02 | MTR_6g065120 | 34.969 | 163 | 98 | 4 | 2 | 160 | 180 | 338 | 5.57e-21 | 87.8 |
| MsG0880043428.01.T02 | MTR_6g065430 | 35.625 | 160 | 95 | 3 | 2 | 157 | 172 | 327 | 3.49e-20 | 85.5 |
| MsG0880043428.01.T02 | MTR_6g065110 | 32.919 | 161 | 102 | 3 | 2 | 159 | 179 | 336 | 1.50e-19 | 84.0 |
| MsG0880043428.01.T02 | MTR_1g111280 | 37.255 | 153 | 88 | 4 | 2 | 149 | 180 | 329 | 6.68e-19 | 82.0 |
| MsG0880043428.01.T02 | MTR_6g065270 | 32.530 | 166 | 98 | 5 | 2 | 160 | 180 | 338 | 4.15e-18 | 80.1 |
| MsG0880043428.01.T02 | MTR_7g406880 | 33.908 | 174 | 97 | 5 | 2 | 158 | 178 | 350 | 6.25e-18 | 79.3 |
| MsG0880043428.01.T02 | MTR_1g082210 | 35.119 | 168 | 88 | 6 | 1 | 159 | 187 | 342 | 8.94e-18 | 79.0 |
| MsG0880043428.01.T02 | MTR_1g089310 | 36.957 | 138 | 73 | 4 | 2 | 134 | 188 | 316 | 6.58e-17 | 76.6 |
| MsG0880043428.01.T02 | MTR_1g089310 | 36.957 | 138 | 73 | 4 | 2 | 134 | 204 | 332 | 6.76e-17 | 76.6 |
| MsG0880043428.01.T02 | MTR_7g093950 | 32.317 | 164 | 99 | 4 | 2 | 158 | 189 | 347 | 1.37e-16 | 75.9 |
| MsG0880043428.01.T02 | MTR_4g050140 | 34.375 | 160 | 100 | 3 | 2 | 157 | 157 | 315 | 1.78e-16 | 75.1 |
| MsG0880043428.01.T02 | MTR_7g107040 | 31.737 | 167 | 106 | 5 | 1 | 162 | 158 | 321 | 1.22e-14 | 70.1 |
| MsG0880043428.01.T02 | MTR_1g012430 | 33.871 | 124 | 77 | 4 | 2 | 123 | 150 | 270 | 1.26e-13 | 67.4 |
| MsG0880043428.01.T01 | MTR_8g035520 | 95.614 | 228 | 10 | 0 | 1 | 228 | 102 | 329 | 2.43e-164 | 457 |
| MsG0880043428.01.T01 | MTR_8g035540 | 79.372 | 223 | 46 | 0 | 3 | 225 | 96 | 318 | 2.87e-131 | 373 |
| MsG0880043428.01.T01 | MTR_6g464510 | 52.632 | 228 | 94 | 4 | 3 | 224 | 108 | 327 | 8.45e-80 | 242 |
| MsG0880043428.01.T01 | MTR_6g464280 | 55.263 | 228 | 89 | 4 | 3 | 224 | 105 | 325 | 1.60e-78 | 239 |
| MsG0880043428.01.T01 | MTR_6g464320 | 51.515 | 231 | 99 | 4 | 3 | 227 | 106 | 329 | 4.82e-73 | 225 |
| MsG0880043428.01.T01 | MTR_8g035500 | 46.154 | 221 | 117 | 2 | 3 | 223 | 99 | 317 | 1.50e-67 | 211 |
| MsG0880043428.01.T01 | MTR_0007s0520 | 48.485 | 231 | 104 | 4 | 3 | 225 | 103 | 326 | 3.67e-67 | 210 |
| MsG0880043428.01.T01 | MTR_1g102160 | 44.589 | 231 | 114 | 5 | 3 | 225 | 103 | 327 | 1.26e-62 | 198 |
| MsG0880043428.01.T01 | MTR_1g102170 | 46.429 | 224 | 104 | 4 | 3 | 219 | 120 | 334 | 4.53e-60 | 192 |
| MsG0880043428.01.T01 | MTR_6g464120 | 48.148 | 216 | 104 | 4 | 3 | 215 | 107 | 317 | 9.48e-60 | 196 |
| MsG0880043428.01.T01 | MTR_0007s0480 | 50.292 | 171 | 76 | 3 | 63 | 225 | 3 | 172 | 1.96e-48 | 157 |
| MsG0880043428.01.T01 | MTR_7g107040 | 34.764 | 233 | 144 | 5 | 1 | 228 | 92 | 321 | 1.09e-37 | 134 |
| MsG0880043428.01.T01 | MTR_1g111280 | 36.986 | 219 | 128 | 5 | 4 | 215 | 114 | 329 | 4.62e-36 | 130 |
| MsG0880043428.01.T01 | MTR_6g065120 | 35.965 | 228 | 131 | 6 | 3 | 226 | 122 | 338 | 6.13e-35 | 127 |
| MsG0880043428.01.T01 | MTR_6g065370 | 35.526 | 228 | 132 | 6 | 3 | 226 | 120 | 336 | 8.55e-35 | 127 |
| MsG0880043428.01.T01 | MTR_6g065110 | 34.513 | 226 | 135 | 5 | 3 | 225 | 121 | 336 | 1.18e-34 | 126 |
| MsG0880043428.01.T01 | MTR_6g065400 | 35.088 | 228 | 133 | 6 | 3 | 226 | 120 | 336 | 2.63e-34 | 125 |
| MsG0880043428.01.T01 | MTR_7g406880 | 34.934 | 229 | 130 | 6 | 13 | 224 | 124 | 350 | 3.60e-33 | 122 |
| MsG0880043428.01.T01 | MTR_4g050140 | 35.111 | 225 | 140 | 4 | 3 | 223 | 93 | 315 | 4.57e-33 | 122 |
| MsG0880043428.01.T01 | MTR_6g065430 | 35.556 | 225 | 130 | 5 | 3 | 223 | 114 | 327 | 1.79e-32 | 120 |
| MsG0880043428.01.T01 | MTR_6g065270 | 34.199 | 231 | 131 | 7 | 3 | 226 | 122 | 338 | 4.22e-32 | 119 |
| MsG0880043428.01.T01 | MTR_1g012430 | 35.484 | 186 | 110 | 6 | 6 | 189 | 93 | 270 | 2.26e-28 | 109 |
| MsG0880043428.01.T01 | MTR_1g089310 | 33.824 | 204 | 112 | 6 | 3 | 200 | 130 | 316 | 6.74e-28 | 108 |
| MsG0880043428.01.T01 | MTR_1g089310 | 34.314 | 204 | 111 | 6 | 3 | 200 | 146 | 332 | 7.88e-28 | 108 |
| MsG0880043428.01.T01 | MTR_1g082210 | 32.189 | 233 | 128 | 8 | 3 | 225 | 130 | 342 | 1.12e-26 | 105 |
| MsG0880043428.01.T01 | MTR_3g012440 | 33.180 | 217 | 130 | 5 | 20 | 225 | 130 | 342 | 6.34e-26 | 103 |
| MsG0880043428.01.T01 | MTR_7g093950 | 29.694 | 229 | 142 | 5 | 3 | 224 | 131 | 347 | 1.25e-25 | 102 |
| MsG0880043428.01.T01 | MTR_6g065340 | 32.456 | 228 | 123 | 8 | 3 | 226 | 131 | 331 | 7.47e-24 | 97.8 |
| MsG0880043428.01.T01 | MTR_4g086510 | 31.579 | 228 | 139 | 7 | 4 | 224 | 101 | 318 | 7.87e-24 | 97.4 |
| MsG0880043428.01.T01 | MTR_5g005920 | 30.172 | 232 | 144 | 8 | 3 | 221 | 106 | 332 | 5.59e-23 | 95.1 |
| MsG0880043428.01.T01 | MTR_3g101420 | 31.466 | 232 | 144 | 8 | 1 | 225 | 93 | 316 | 1.54e-22 | 94.0 |
| MsG0880043428.01.T01 | MTR_7g056207 | 30.837 | 227 | 142 | 6 | 4 | 224 | 133 | 350 | 1.58e-22 | 94.4 |
| MsG0880043428.01.T01 | MTR_4g086540 | 30.222 | 225 | 123 | 7 | 4 | 224 | 108 | 302 | 3.64e-22 | 92.8 |
| MsG0880043428.01.T01 | MTR_6g022710 | 29.008 | 262 | 142 | 8 | 3 | 224 | 185 | 442 | 2.50e-20 | 89.0 |
| MsG0880043428.01.T01 | MTR_4g086480 | 30.556 | 216 | 138 | 6 | 12 | 223 | 111 | 318 | 1.81e-19 | 85.5 |
| MsG0880043428.01.T01 | MTR_1g105020 | 29.825 | 228 | 147 | 8 | 4 | 224 | 99 | 320 | 5.75e-18 | 81.3 |
| MsG0880043428.01.T01 | MTR_7g051020 | 39.048 | 105 | 62 | 2 | 1 | 103 | 99 | 203 | 2.18e-17 | 79.7 |
| MsG0880043428.01.T01 | MTR_1g104933 | 41.935 | 93 | 52 | 2 | 13 | 103 | 106 | 198 | 3.05e-16 | 76.6 |
| MsG0880043428.01.T01 | MTR_4g086470 | 31.944 | 216 | 135 | 8 | 12 | 223 | 110 | 317 | 6.26e-16 | 75.5 |
| MsG0880043428.01.T01 | MTR_3g450680 | 30.342 | 234 | 136 | 9 | 6 | 223 | 109 | 331 | 1.42e-15 | 74.7 |
| MsG0880043428.01.T01 | MTR_1g104930 | 39.216 | 102 | 60 | 2 | 4 | 103 | 99 | 200 | 1.77e-15 | 74.3 |
| MsG0880043428.01.T01 | MTR_5g005900 | 34.821 | 112 | 68 | 2 | 1 | 107 | 97 | 208 | 4.19e-15 | 73.6 |
| MsG0880043428.01.T01 | MTR_5g005850 | 37.736 | 106 | 61 | 3 | 12 | 112 | 112 | 217 | 4.93e-14 | 70.5 |
| MsG0880043428.01.T01 | MTR_8g040860 | 34.513 | 113 | 66 | 3 | 5 | 112 | 108 | 217 | 2.56e-11 | 62.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880043428.01.T02 | AT2G45600 | 57.669 | 163 | 68 | 1 | 1 | 162 | 158 | 320 | 2.15e-61 | 192 |
| MsG0880043428.01.T02 | AT2G45610 | 52.866 | 157 | 73 | 1 | 2 | 158 | 169 | 324 | 1.35e-50 | 165 |
| MsG0880043428.01.T02 | AT5G62180 | 49.091 | 165 | 78 | 4 | 1 | 160 | 163 | 326 | 3.63e-44 | 148 |
| MsG0880043428.01.T02 | AT5G16080 | 37.931 | 145 | 81 | 4 | 2 | 142 | 182 | 321 | 1.54e-19 | 84.0 |
| MsG0880043428.01.T02 | AT1G68620 | 40.517 | 116 | 63 | 3 | 2 | 115 | 174 | 285 | 2.08e-17 | 78.2 |
| MsG0880043428.01.T02 | AT3G63010 | 32.143 | 168 | 95 | 5 | 2 | 161 | 189 | 345 | 7.21e-16 | 73.9 |
| MsG0880043428.01.T02 | AT5G06570 | 34.839 | 155 | 93 | 4 | 1 | 149 | 181 | 333 | 3.87e-15 | 71.6 |
| MsG0880043428.01.T02 | AT5G06570 | 34.839 | 155 | 93 | 4 | 1 | 149 | 181 | 333 | 3.87e-15 | 71.6 |
| MsG0880043428.01.T02 | AT5G06570 | 34.839 | 155 | 93 | 4 | 1 | 149 | 166 | 318 | 4.22e-15 | 71.6 |
| MsG0880043428.01.T02 | AT5G06570 | 34.839 | 155 | 93 | 4 | 1 | 149 | 166 | 318 | 4.22e-15 | 71.6 |
| MsG0880043428.01.T02 | AT5G27320 | 32.609 | 138 | 79 | 3 | 2 | 134 | 187 | 315 | 3.21e-14 | 69.3 |
| MsG0880043428.01.T02 | AT3G05120 | 43.750 | 80 | 42 | 1 | 2 | 81 | 189 | 265 | 3.71e-13 | 66.2 |
| MsG0880043428.01.T01 | AT2G45600 | 54.310 | 232 | 102 | 2 | 1 | 228 | 89 | 320 | 2.57e-88 | 264 |
| MsG0880043428.01.T01 | AT2G45610 | 50.893 | 224 | 109 | 1 | 1 | 224 | 102 | 324 | 3.21e-79 | 241 |
| MsG0880043428.01.T01 | AT5G62180 | 48.035 | 229 | 107 | 5 | 3 | 226 | 105 | 326 | 8.68e-66 | 206 |
| MsG0880043428.01.T01 | AT5G16080 | 38.278 | 209 | 120 | 4 | 4 | 208 | 118 | 321 | 2.81e-36 | 130 |
| MsG0880043428.01.T01 | AT5G06570 | 38.462 | 221 | 125 | 6 | 3 | 215 | 101 | 318 | 2.28e-34 | 125 |
| MsG0880043428.01.T01 | AT5G06570 | 38.462 | 221 | 125 | 6 | 3 | 215 | 101 | 318 | 2.28e-34 | 125 |
| MsG0880043428.01.T01 | AT5G06570 | 38.462 | 221 | 125 | 6 | 3 | 215 | 116 | 333 | 2.46e-34 | 125 |
| MsG0880043428.01.T01 | AT5G06570 | 38.462 | 221 | 125 | 6 | 3 | 215 | 116 | 333 | 2.46e-34 | 125 |
| MsG0880043428.01.T01 | AT1G68620 | 40.449 | 178 | 95 | 6 | 6 | 181 | 117 | 285 | 4.14e-31 | 117 |
| MsG0880043428.01.T01 | AT3G63010 | 32.051 | 234 | 131 | 7 | 3 | 227 | 131 | 345 | 2.40e-28 | 110 |
| MsG0880043428.01.T01 | AT5G23530 | 32.174 | 230 | 136 | 7 | 1 | 223 | 110 | 326 | 7.33e-26 | 103 |
| MsG0880043428.01.T01 | AT5G27320 | 33.014 | 209 | 114 | 6 | 3 | 202 | 129 | 320 | 1.83e-24 | 99.4 |
| MsG0880043428.01.T01 | AT3G05120 | 32.995 | 197 | 119 | 4 | 3 | 198 | 131 | 315 | 2.63e-22 | 93.6 |
| MsG0880043428.01.T01 | AT5G14310 | 29.699 | 266 | 140 | 8 | 3 | 224 | 176 | 438 | 6.25e-22 | 93.6 |
| MsG0880043428.01.T01 | AT1G47480 | 30.088 | 226 | 136 | 8 | 9 | 227 | 103 | 313 | 7.92e-19 | 83.6 |
| MsG0880043428.01.T01 | AT3G27320 | 30.387 | 181 | 92 | 3 | 3 | 149 | 159 | 339 | 3.36e-18 | 82.8 |
| MsG0880043428.01.T01 | AT3G27320 | 30.387 | 181 | 92 | 3 | 3 | 149 | 191 | 371 | 3.87e-18 | 82.8 |
| MsG0880043428.01.T01 | AT1G49640 | 43.011 | 93 | 46 | 2 | 13 | 105 | 109 | 194 | 1.42e-16 | 77.4 |
| MsG0880043428.01.T01 | AT3G48700 | 33.333 | 135 | 75 | 4 | 13 | 139 | 109 | 236 | 4.55e-14 | 70.5 |
| MsG0880043428.01.T01 | AT1G19190 | 35.484 | 93 | 57 | 2 | 13 | 103 | 107 | 198 | 4.16e-13 | 67.8 |
| MsG0880043428.01.T01 | AT3G48690 | 31.298 | 131 | 82 | 4 | 13 | 139 | 106 | 232 | 1.73e-12 | 65.9 |
| MsG0880043428.01.T01 | AT1G49660 | 36.170 | 94 | 59 | 1 | 13 | 106 | 107 | 199 | 1.08e-11 | 63.5 |
| MsG0880043428.01.T01 | AT2G03550 | 37.500 | 88 | 54 | 1 | 16 | 103 | 106 | 192 | 2.00e-11 | 62.8 |
| MsG0880043428.01.T01 | AT1G49650 | 35.484 | 93 | 58 | 2 | 13 | 105 | 163 | 253 | 6.86e-11 | 61.6 |
Find 64 sgRNAs with CRISPR-Local
Find 79 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGGCTACAACAATCGGTATT+TGG | 0.173341 | 8:+24644419 | None:intergenic |
| AAAGGAGCTAGTGAAGATTT+TGG | 0.262688 | 8:-24643909 | MsG0880043428.01.T01:CDS |
| TATATCACATCCGAAACTTT+TGG | 0.276208 | 8:+24644004 | None:intergenic |
| CTGCCGAGGTGCTTTGTTAA+TGG | 0.290098 | 8:-24643962 | MsG0880043428.01.T01:CDS |
| CATAAGATCACTTGCTGATA+AGG | 0.336937 | 8:+24644078 | None:intergenic |
| GGCTACAACAATCGGTATTT+GGG | 0.352791 | 8:+24644420 | None:intergenic |
| CTTTGATAACTGCTTCTTGA+TGG | 0.402538 | 8:-24644263 | MsG0880043428.01.T01:CDS |
| AAGCATTAATTGAGTACGTT+AGG | 0.420290 | 8:-24643805 | MsG0880043428.01.T01:CDS |
| ATAAGCCCTTGGATCTTAAG+AGG | 0.421207 | 8:+24644175 | None:intergenic |
| GTATTGTAATCCAAAAGTTT+CGG | 0.440947 | 8:-24644014 | MsG0880043428.01.T01:CDS |
| ATTAATGCTTGTGCCTTAGC+AGG | 0.443498 | 8:+24643818 | None:intergenic |
| TGGCTACGGTGGGGATCCCT+TGG | 0.444444 | 8:-24643942 | MsG0880043428.01.T01:CDS |
| AAAGTTTCGGATGTGATATA+CGG | 0.451670 | 8:-24644001 | MsG0880043428.01.T01:CDS |
| CGTTAGGAAATTTATTTCCT+CGG | 0.460385 | 8:-24643789 | MsG0880043428.01.T01:CDS |
| TATCAGCAAGTGATCTTATG+TGG | 0.466303 | 8:-24644075 | MsG0880043428.01.T01:CDS |
| ATAAGATCACTTGCTGATAA+GGG | 0.469063 | 8:+24644079 | None:intergenic |
| CTACGGTGGGGATCCCTTGG+TGG | 0.471238 | 8:-24643939 | MsG0880043428.01.T01:CDS |
| GTGGGGATCCCTTGGTGGAT+AGG | 0.478308 | 8:-24643934 | MsG0880043428.01.T01:CDS |
| GCAGCAGGGAGACGGTGCTC+TGG | 0.489342 | 8:+24644376 | None:intergenic |
| AGGTGCTTTGTTAATGGCTA+CGG | 0.498754 | 8:-24643956 | MsG0880043428.01.T01:CDS |
| GTGATATACGGTGAGAAGAT+CGG | 0.499961 | 8:-24643989 | MsG0880043428.01.T01:CDS |
| CGGTATTTGGGCGGCGAAGG+TGG | 0.500026 | 8:+24644432 | None:intergenic |
| TTTGCCTGAGGGCAAAGATC+GGG | 0.502336 | 8:-24644044 | MsG0880043428.01.T01:CDS |
| GAGCATTCATTATAAGCCCT+TGG | 0.503256 | 8:+24644164 | None:intergenic |
| ACAGCGTCATCATAGGCAGC+AGG | 0.503505 | 8:+24644361 | None:intergenic |
| AATCGGTATTTGGGCGGCGA+AGG | 0.504867 | 8:+24644429 | None:intergenic |
| CTCTCTCCTCTTAAGATCCA+AGG | 0.508284 | 8:-24644181 | MsG0880043428.01.T01:CDS |
| TTCTTGATGGGAAGTAGTGC+AGG | 0.508384 | 8:-24644250 | MsG0880043428.01.T01:CDS |
| TGAGGATGGGTTTCATGCTG+TGG | 0.514157 | 8:-24643852 | MsG0880043428.01.T01:CDS |
| TCCCTTGGTGGATAGGCAAA+AGG | 0.515897 | 8:-24643927 | MsG0880043428.01.T01:CDS |
| TCAACTGAGGCTACAACAAT+CGG | 0.516064 | 8:+24644412 | None:intergenic |
| GAAGCTCGGATTCTGTCCTC+TGG | 0.518185 | 8:+24644125 | None:intergenic |
| ATCCAAGTGTCTCTGATCCA+TGG | 0.519484 | 8:-24644303 | MsG0880043428.01.T01:CDS |
| TGAATCAACAGCGTCATCAT+AGG | 0.525186 | 8:+24644354 | None:intergenic |
| TTTGATAACTGCTTCTTGAT+GGG | 0.528311 | 8:-24644262 | MsG0880043428.01.T01:CDS |
| TGCTTTGTTAATGGCTACGG+TGG | 0.528480 | 8:-24643953 | MsG0880043428.01.T01:CDS |
| TGATCCCGATCTTTGCCCTC+AGG | 0.528666 | 8:+24644040 | None:intergenic |
| CTTTGCCTGAGGGCAAAGAT+CGG | 0.530032 | 8:-24644045 | MsG0880043428.01.T01:CDS |
| TCCACGTTGTCTCTAATCCA+TGG | 0.538847 | 8:+24644286 | None:intergenic |
| GCTTTGTTAATGGCTACGGT+GGG | 0.539699 | 8:-24643952 | MsG0880043428.01.T01:CDS |
| CATCATAGGCAGCAGGGAGA+CGG | 0.539880 | 8:+24644368 | None:intergenic |
| ACGCTGTTGATTCACTGCTC+TGG | 0.544781 | 8:-24644345 | MsG0880043428.01.T01:CDS |
| CGGCGAAGGTGGAACAACAT+TGG | 0.547001 | 8:+24644443 | None:intergenic |
| AGAAGATCGGACGGCTGCCG+AGG | 0.559266 | 8:-24643976 | MsG0880043428.01.T01:CDS |
| GTGGGCTCTATCTTTGCCTG+AGG | 0.562373 | 8:-24644056 | MsG0880043428.01.T01:CDS |
| TGGGCTCTATCTTTGCCTGA+GGG | 0.574915 | 8:-24644055 | MsG0880043428.01.T01:CDS |
| GGAGGCACGTGGGGTGCACG+TGG | 0.576950 | 8:-24643888 | MsG0880043428.01.T01:CDS |
| ATCGTTAATGAAACGAAGCT+CGG | 0.583980 | 8:+24644111 | None:intergenic |
| ATCCATGGATCAGAGACACT+TGG | 0.585274 | 8:+24644301 | None:intergenic |
| GGAACTCTTTGATCCTGCTA+AGG | 0.595599 | 8:-24643831 | MsG0880043428.01.T01:CDS |
| GACGGTGCTCTGGTGTGAGA+CGG | 0.599030 | 8:+24644386 | None:intergenic |
| ATCAGCAAGTGATCTTATGT+GGG | 0.600680 | 8:-24644074 | MsG0880043428.01.T01:CDS |
| TCTCTCCTCTTAAGATCCAA+GGG | 0.605883 | 8:-24644180 | MsG0880043428.01.T01:CDS |
| GGTAACATAGCGTATTTCGC+AGG | 0.611649 | 8:-24644226 | MsG0880043428.01.T01:CDS |
| TCTTAAGAGGAGAGAGATCG+AGG | 0.616358 | 8:+24644188 | None:intergenic |
| CTTTGTTAATGGCTACGGTG+GGG | 0.624764 | 8:-24643951 | MsG0880043428.01.T01:CDS |
| CAGCGTCATCATAGGCAGCA+GGG | 0.627063 | 8:+24644362 | None:intergenic |
| TACAACAATCGGTATTTGGG+CGG | 0.668880 | 8:+24644423 | None:intergenic |
| TTGATGGGAAGTAGTGCAGG+TGG | 0.669706 | 8:-24644247 | MsG0880043428.01.T01:CDS |
| TATACGGTGAGAAGATCGGA+CGG | 0.716166 | 8:-24643985 | MsG0880043428.01.T01:CDS |
| TGTGAGACGGTGATCAACTG+AGG | 0.747102 | 8:+24644399 | None:intergenic |
| TAGCCATTAACAAAGCACCT+CGG | 0.752093 | 8:+24643959 | None:intergenic |
| TCCATGGATTAGAGACAACG+TGG | 0.752565 | 8:-24644287 | MsG0880043428.01.T01:CDS |
| AGAGGACTAAGTATCAACCG+AGG | 0.780180 | 8:+24643772 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | GTATTGTAATCCAAAAGTTT+CGG | - | Chr8:24644206-24644225 | MsG0880043428.01.T01:CDS | 25.0% |
| !!! | ATAATGAATGCTCCATTTTT+TGG | - | Chr8:24644066-24644085 | MsG0880043428.01.T01:CDS | 25.0% |
| AAAGTTTCGGATGTGATATA+CGG | - | Chr8:24644219-24644238 | MsG0880043428.01.T01:CDS | 30.0% | |
| AAGCATTAATTGAGTACGTT+AGG | - | Chr8:24644415-24644434 | MsG0880043428.01.T01:CDS | 30.0% | |
| CGTTAGGAAATTTATTTCCT+CGG | - | Chr8:24644431-24644450 | MsG0880043428.01.T01:CDS | 30.0% | |
| ! | ATAAGATCACTTGCTGATAA+GGG | + | Chr8:24644144-24644163 | None:intergenic | 30.0% |
| ! | TATATCACATCCGAAACTTT+TGG | + | Chr8:24644219-24644238 | None:intergenic | 30.0% |
| ! | TTTGATAACTGCTTCTTGAT+GGG | - | Chr8:24643958-24643977 | MsG0880043428.01.T01:CDS | 30.0% |
| ATCAGCAAGTGATCTTATGT+GGG | - | Chr8:24644146-24644165 | MsG0880043428.01.T01:CDS | 35.0% | |
| ATCGTTAATGAAACGAAGCT+CGG | + | Chr8:24644112-24644131 | None:intergenic | 35.0% | |
| TATCAGCAAGTGATCTTATG+TGG | - | Chr8:24644145-24644164 | MsG0880043428.01.T01:CDS | 35.0% | |
| ! | AAAGGAGCTAGTGAAGATTT+TGG | - | Chr8:24644311-24644330 | MsG0880043428.01.T01:CDS | 35.0% |
| ! | CATAAGATCACTTGCTGATA+AGG | + | Chr8:24644145-24644164 | None:intergenic | 35.0% |
| ! | CTTTGATAACTGCTTCTTGA+TGG | - | Chr8:24643957-24643976 | MsG0880043428.01.T01:CDS | 35.0% |
| !!! | AGTCTGTTTTTTGTGAGGAT+GGG | - | Chr8:24644355-24644374 | MsG0880043428.01.T01:CDS | 35.0% |
| !!! | ATGAATGCTCCATTTTTTGG+TGG | - | Chr8:24644069-24644088 | MsG0880043428.01.T01:CDS | 35.0% |
| !!! | TGAATGCTCCATTTTTTGGT+GGG | - | Chr8:24644070-24644089 | MsG0880043428.01.T01:CDS | 35.0% |
| AGGCTACAACAATCGGTATT+TGG | + | Chr8:24643804-24643823 | None:intergenic | 40.0% | |
| ATAAGCCCTTGGATCTTAAG+AGG | + | Chr8:24644048-24644067 | None:intergenic | 40.0% | |
| ATTAATGCTTGTGCCTTAGC+AGG | + | Chr8:24644405-24644424 | None:intergenic | 40.0% | |
| GAGCATTCATTATAAGCCCT+TGG | + | Chr8:24644059-24644078 | None:intergenic | 40.0% | |
| GGCTACAACAATCGGTATTT+GGG | + | Chr8:24643803-24643822 | None:intergenic | 40.0% | |
| GTGATATACGGTGAGAAGAT+CGG | - | Chr8:24644231-24644250 | MsG0880043428.01.T01:CDS | 40.0% | |
| TACAACAATCGGTATTTGGG+CGG | + | Chr8:24643800-24643819 | None:intergenic | 40.0% | |
| TCAACTGAGGCTACAACAAT+CGG | + | Chr8:24643811-24643830 | None:intergenic | 40.0% | |
| TCTCTCCTCTTAAGATCCAA+GGG | - | Chr8:24644040-24644059 | MsG0880043428.01.T01:CDS | 40.0% | |
| TGAATCAACAGCGTCATCAT+AGG | + | Chr8:24643869-24643888 | None:intergenic | 40.0% | |
| ! | AGGTGCTTTGTTAATGGCTA+CGG | - | Chr8:24644264-24644283 | MsG0880043428.01.T01:CDS | 40.0% |
| ! | TAGCCATTAACAAAGCACCT+CGG | + | Chr8:24644264-24644283 | None:intergenic | 40.0% |
| !!! | GAATGCTCCATTTTTTGGTG+GGG | - | Chr8:24644071-24644090 | MsG0880043428.01.T01:CDS | 40.0% |
| !!! | GAGTCTGTTTTTTGTGAGGA+TGG | - | Chr8:24644354-24644373 | MsG0880043428.01.T01:CDS | 40.0% |
| ATCCAAGTGTCTCTGATCCA+TGG | - | Chr8:24643917-24643936 | MsG0880043428.01.T01:CDS | 45.0% | |
| ATCCATGGATCAGAGACACT+TGG | + | Chr8:24643922-24643941 | None:intergenic | 45.0% | |
| CTCTCTCCTCTTAAGATCCA+AGG | - | Chr8:24644039-24644058 | MsG0880043428.01.T01:CDS | 45.0% | |
| CTTTGTTAATGGCTACGGTG+GGG | - | Chr8:24644269-24644288 | MsG0880043428.01.T01:CDS | 45.0% | |
| GGAACTCTTTGATCCTGCTA+AGG | - | Chr8:24644389-24644408 | MsG0880043428.01.T01:CDS | 45.0% | |
| GGTAACATAGCGTATTTCGC+AGG | - | Chr8:24643994-24644013 | MsG0880043428.01.T01:CDS | 45.0% | |
| TATACGGTGAGAAGATCGGA+CGG | - | Chr8:24644235-24644254 | MsG0880043428.01.T01:CDS | 45.0% | |
| TCCACGTTGTCTCTAATCCA+TGG | + | Chr8:24643937-24643956 | None:intergenic | 45.0% | |
| TCTTAAGAGGAGAGAGATCG+AGG | + | Chr8:24644035-24644054 | None:intergenic | 45.0% | |
| TTCTTGATGGGAAGTAGTGC+AGG | - | Chr8:24643970-24643989 | MsG0880043428.01.T01:CDS | 45.0% | |
| ! | AGACACTTGGATTTTGCGCT+TGG | + | Chr8:24643909-24643928 | None:intergenic | 45.0% |
| ! | GACACTTGGATTTTGCGCTT+GGG | + | Chr8:24643908-24643927 | None:intergenic | 45.0% |
| ! | GCTTTGTTAATGGCTACGGT+GGG | - | Chr8:24644268-24644287 | MsG0880043428.01.T01:CDS | 45.0% |
| ! | GGAGCTAGTGAAGATTTTGG+AGG | - | Chr8:24644314-24644333 | MsG0880043428.01.T01:CDS | 45.0% |
| ! | TCCTTTTGCCTATCCACCAA+GGG | + | Chr8:24644297-24644316 | None:intergenic | 45.0% |
| ! | TGCTTTGTTAATGGCTACGG+TGG | - | Chr8:24644267-24644286 | MsG0880043428.01.T01:CDS | 45.0% |
| !! | TCCATGGATTAGAGACAACG+TGG | - | Chr8:24643933-24643952 | MsG0880043428.01.T01:CDS | 45.0% |
| !! | TGAAGATTTTGGAGGCACGT+GGG | - | Chr8:24644322-24644341 | MsG0880043428.01.T01:CDS | 45.0% |
| !!! | CGTGGAGTCTGTTTTTTGTG+AGG | - | Chr8:24644350-24644369 | MsG0880043428.01.T01:CDS | 45.0% |
| CTCTGGACCCCACCAAAAAA+TGG | + | Chr8:24644081-24644100 | None:intergenic | 50.0% | |
| CTTTGCCTGAGGGCAAAGAT+CGG | - | Chr8:24644175-24644194 | MsG0880043428.01.T01:CDS | 50.0% | |
| TGGGCTCTATCTTTGCCTGA+GGG | - | Chr8:24644165-24644184 | MsG0880043428.01.T01:CDS | 50.0% | |
| TGTGAGACGGTGATCAACTG+AGG | + | Chr8:24643824-24643843 | None:intergenic | 50.0% | |
| TTGATGGGAAGTAGTGCAGG+TGG | - | Chr8:24643973-24643992 | MsG0880043428.01.T01:CDS | 50.0% | |
| TTTGCCTGAGGGCAAAGATC+GGG | - | Chr8:24644176-24644195 | MsG0880043428.01.T01:CDS | 50.0% | |
| ! | CTCCTTTTGCCTATCCACCA+AGG | + | Chr8:24644298-24644317 | None:intergenic | 50.0% |
| ! | CTGCCGAGGTGCTTTGTTAA+TGG | - | Chr8:24644258-24644277 | MsG0880043428.01.T01:CDS | 50.0% |
| ! | TCCCTTGGTGGATAGGCAAA+AGG | - | Chr8:24644293-24644312 | MsG0880043428.01.T01:CDS | 50.0% |
| ! | TGAGGATGGGTTTCATGCTG+TGG | - | Chr8:24644368-24644387 | MsG0880043428.01.T01:CDS | 50.0% |
| !! | ACGCTGTTGATTCACTGCTC+TGG | - | Chr8:24643875-24643894 | MsG0880043428.01.T01:CDS | 50.0% |
| !! | GAAGATTTTGGAGGCACGTG+GGG | - | Chr8:24644323-24644342 | MsG0880043428.01.T01:CDS | 50.0% |
| !! | GTGAAGATTTTGGAGGCACG+TGG | - | Chr8:24644321-24644340 | MsG0880043428.01.T01:CDS | 50.0% |
| !!! | CATTTTTTGGTGGGGTCCAG+AGG | - | Chr8:24644079-24644098 | MsG0880043428.01.T01:CDS | 50.0% |
| AATCGGTATTTGGGCGGCGA+AGG | + | Chr8:24643794-24643813 | None:intergenic | 55.0% | |
| ACAGCGTCATCATAGGCAGC+AGG | + | Chr8:24643862-24643881 | None:intergenic | 55.0% | |
| CAGCGTCATCATAGGCAGCA+GGG | + | Chr8:24643861-24643880 | None:intergenic | 55.0% | |
| CATCATAGGCAGCAGGGAGA+CGG | + | Chr8:24643855-24643874 | None:intergenic | 55.0% | |
| GAAGCTCGGATTCTGTCCTC+TGG | + | Chr8:24644098-24644117 | None:intergenic | 55.0% | |
| GTGGGCTCTATCTTTGCCTG+AGG | - | Chr8:24644164-24644183 | MsG0880043428.01.T01:CDS | 55.0% | |
| TGATCCCGATCTTTGCCCTC+AGG | + | Chr8:24644183-24644202 | None:intergenic | 55.0% | |
| GACGGTGCTCTGGTGTGAGA+CGG | + | Chr8:24643837-24643856 | None:intergenic | 60.0% | |
| ! | GTGGGGATCCCTTGGTGGAT+AGG | - | Chr8:24644286-24644305 | MsG0880043428.01.T01:CDS | 60.0% |
| AGAAGATCGGACGGCTGCCG+AGG | - | Chr8:24644244-24644263 | MsG0880043428.01.T01:CDS | 65.0% | |
| CGGTATTTGGGCGGCGAAGG+TGG | + | Chr8:24643791-24643810 | None:intergenic | 65.0% | |
| CTACGGTGGGGATCCCTTGG+TGG | - | Chr8:24644281-24644300 | MsG0880043428.01.T01:CDS | 65.0% | |
| TGGCTACGGTGGGGATCCCT+TGG | - | Chr8:24644278-24644297 | MsG0880043428.01.T01:CDS | 65.0% | |
| GCAGCAGGGAGACGGTGCTC+TGG | + | Chr8:24643847-24643866 | None:intergenic | 70.0% | |
| ! | GGAGGCACGTGGGGTGCACG+TGG | - | Chr8:24644332-24644351 | MsG0880043428.01.T01:CDS | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr8 | gene | 24643778 | 24644464 | 24643778 | ID=MsG0880043428.01;Name=MsG0880043428.01 |
| Chr8 | mRNA | 24643778 | 24644464 | 24643778 | ID=MsG0880043428.01.T01;Parent=MsG0880043428.01;Name=MsG0880043428.01.T01;_AED=0.49;_eAED=0.49;_QI=0|-1|0|1|-1|1|1|0|228 |
| Chr8 | exon | 24643778 | 24644464 | 24643778 | ID=MsG0880043428.01.T01:exon:9146;Parent=MsG0880043428.01.T01 |
| Chr8 | CDS | 24643778 | 24644464 | 24643778 | ID=MsG0880043428.01.T01:cds;Parent=MsG0880043428.01.T01 |
| Chr8 | mRNA | 24643778 | 24644266 | 24643778 | ID=MsG0880043428.01.T02;Parent=MsG0880043428.01;Name=MsG0880043428.01.T02;_AED=0.49;_eAED=0.49;_QI=0|-1|0|1|-1|0|1|0|162 |
| Chr8 | exon | 24643778 | 24644266 | 24643778 | ID=MsG0880043428.01.T02:exon:9147;Parent=MsG0880043428.01.T02 |
| Chr8 | CDS | 24643778 | 24644266 | 24643778 | ID=MsG0880043428.01.T02:cds;Parent=MsG0880043428.01.T02 |
| Gene Sequence |
| Protein sequence |
