Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780041226.01.T01 | XP_013450274.1 | 90.533 | 169 | 13 | 2 | 1 | 169 | 12 | 177 | 2.50E-101 | 305 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780041226.01.T01 | Q9FG13 | 58.235 | 170 | 67 | 2 | 1 | 167 | 15 | 183 | 1.65E-53 | 176 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780041226.01.T01 | A0A072U3J9 | 90.533 | 169 | 13 | 2 | 1 | 169 | 12 | 177 | 1.20e-101 | 305 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180000762.01 | MsG0780041226.01 | 0.801995 | 6.922097e-49 | 3.352628e-46 |
| MsG0180001513.01 | MsG0780041226.01 | 0.802867 | 4.576818e-49 | 2.266599e-46 |
| MsG0180001996.01 | MsG0780041226.01 | 0.803416 | 3.523170e-49 | 1.769462e-46 |
| MsG0180003375.01 | MsG0780041226.01 | 0.831567 | 1.551616e-55 | 1.690369e-52 |
| MsG0180003484.01 | MsG0780041226.01 | 0.800338 | 1.510144e-48 | 7.014080e-46 |
| MsG0180004121.01 | MsG0780041226.01 | 0.833678 | 4.646918e-56 | 5.389829e-53 |
| MsG0180005127.01 | MsG0780041226.01 | 0.879125 | 1.608529e-69 | 8.617605e-66 |
| MsG0180005146.01 | MsG0780041226.01 | 0.881883 | 1.657172e-70 | 9.877438e-67 |
| MsG0180005822.01 | MsG0780041226.01 | -0.824209 | 9.137132e-54 | 8.040502e-51 |
| MsG0180006010.01 | MsG0780041226.01 | 0.832037 | 1.188091e-55 | 1.312370e-52 |
| MsG0780040991.01 | MsG0780041226.01 | 0.816951 | 4.244430e-52 | 3.050297e-49 |
| MsG0780041225.01 | MsG0780041226.01 | 0.900501 | 6.876730e-78 | 8.920545e-74 |
| MsG0780041226.01 | MsG0780041447.01 | 0.820113 | 8.141742e-53 | 6.385489e-50 |
| MsG0780041226.01 | MsG0880041847.01 | -0.802225 | 6.206378e-49 | 3.023681e-46 |
| MsG0780041226.01 | MsG0880043317.01 | 0.802432 | 5.627357e-49 | 2.756193e-46 |
| MsG0780041226.01 | MsG0880046212.01 | 0.806913 | 6.530243e-50 | 3.587962e-47 |
| MsG0780041226.01 | MsG0880046267.01 | -0.805971 | 1.031822e-49 | 5.534265e-47 |
| MsG0780041226.01 | MsG0880046402.01 | 0.833349 | 5.613001e-56 | 6.447401e-53 |
| MsG0780041226.01 | MsG0880046838.01 | -0.801615 | 8.280789e-49 | 3.972213e-46 |
| MsG0780041226.01 | MsG0880047664.01 | 0.813048 | 3.117063e-51 | 2.014072e-48 |
| MsG0780041226.01 | MsG0880047714.01 | 0.819140 | 1.357695e-52 | 1.036110e-49 |
| MsG0280010623.01 | MsG0780041226.01 | 0.801163 | 1.025018e-48 | 4.861390e-46 |
| MsG0280010644.01 | MsG0780041226.01 | 0.824030 | 1.006471e-53 | 8.811642e-51 |
| MsG0280011271.01 | MsG0780041226.01 | 0.813598 | 2.360896e-51 | 1.548496e-48 |
| MsG0680032026.01 | MsG0780041226.01 | 0.822183 | 2.715120e-53 | 2.255927e-50 |
| MsG0680032461.01 | MsG0780041226.01 | 0.841820 | 3.767007e-58 | 5.601362e-55 |
| MsG0680035344.01 | MsG0780041226.01 | 0.807878 | 4.075957e-50 | 2.296300e-47 |
| MsG0480020938.01 | MsG0780041226.01 | -0.809612 | 1.736412e-50 | 1.024306e-47 |
| MsG0480021226.01 | MsG0780041226.01 | 0.801088 | 1.062025e-48 | 5.027539e-46 |
| MsG0480022142.01 | MsG0780041226.01 | 0.813846 | 2.081038e-51 | 1.374112e-48 |
| MsG0480022244.01 | MsG0780041226.01 | -0.806195 | 9.256088e-50 | 4.993044e-47 |
| MsG0480022833.01 | MsG0780041226.01 | 0.810674 | 1.024722e-50 | 6.216111e-48 |
| MsG0480023067.01 | MsG0780041226.01 | 0.828853 | 7.137716e-55 | 7.179999e-52 |
| MsG0580024994.01 | MsG0780041226.01 | 0.831622 | 1.503730e-55 | 1.640796e-52 |
| MsG0580025007.01 | MsG0780041226.01 | 0.828116 | 1.075157e-54 | 1.058744e-51 |
| MsG0580025024.01 | MsG0780041226.01 | 0.836740 | 7.836781e-57 | 9.966365e-54 |
| MsG0580025193.01 | MsG0780041226.01 | 0.803824 | 2.898701e-49 | 1.471206e-46 |
| MsG0380015660.01 | MsG0780041226.01 | 0.806992 | 6.283184e-50 | 3.459202e-47 |
| MsG0380016458.01 | MsG0780041226.01 | 0.830346 | 3.092280e-55 | 3.249438e-52 |
| MsG0380016659.01 | MsG0780041226.01 | -0.807590 | 4.692127e-50 | 2.623655e-47 |
| MsG0380017200.01 | MsG0780041226.01 | 0.825559 | 4.387948e-54 | 4.013221e-51 |
| MsG0380017307.01 | MsG0780041226.01 | 0.807809 | 4.215665e-50 | 2.370833e-47 |
| MsG0580028302.01 | MsG0780041226.01 | 0.801919 | 7.174115e-49 | 3.468045e-46 |
| MsG0680030379.01 | MsG0780041226.01 | 0.808331 | 3.264487e-50 | 1.861415e-47 |
| MsG0280007677.01 | MsG0780041226.01 | 0.810532 | 1.100247e-50 | 6.648448e-48 |
| MsG0280008452.01 | MsG0780041226.01 | 0.812029 | 5.207711e-51 | 3.274522e-48 |
| MsG0280009526.01 | MsG0780041226.01 | 0.805518 | 1.284006e-49 | 6.806919e-47 |
| MsG0780038399.01 | MsG0780041226.01 | 0.824840 | 6.490473e-54 | 5.816239e-51 |
| MsG0780039431.01 | MsG0780041226.01 | 0.805994 | 1.019958e-49 | 5.474027e-47 |
| MsG0780039883.01 | MsG0780041226.01 | 0.800156 | 1.644695e-48 | 7.603278e-46 |
| MsG0780039912.01 | MsG0780041226.01 | 0.820076 | 8.301476e-53 | 6.503662e-50 |
| MsG0780039923.01 | MsG0780041226.01 | 0.829672 | 4.515905e-55 | 4.653819e-52 |
| MsG0780039925.01 | MsG0780041226.01 | 0.804811 | 1.805601e-49 | 9.400502e-47 |
| MsG0780040035.01 | MsG0780041226.01 | 0.810372 | 1.190950e-50 | 7.166279e-48 |
PPI
| Gene1 | Gene2 | Type |
|---|---|---|
| MsG0880043428.01 | MsG0780041226.01 | PPI |
| MsG0780041226.01 | MsG0880043429.01 | PPI |
| MsG0780041226.01 | MsG0180005648.01 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780041226.01.T01 | MTR_7g107040 | 90.533 | 169 | 13 | 2 | 1 | 169 | 12 | 177 | 3.03e-105 | 305 |
| MsG0780041226.01.T01 | MTR_4g050140 | 55.090 | 167 | 72 | 3 | 2 | 168 | 11 | 174 | 1.91e-57 | 182 |
| MsG0780041226.01.T01 | MTR_8g035540 | 39.535 | 172 | 89 | 4 | 4 | 165 | 9 | 175 | 3.59e-33 | 120 |
| MsG0780041226.01.T01 | MTR_3g101420 | 37.853 | 177 | 100 | 5 | 3 | 172 | 14 | 187 | 1.87e-32 | 118 |
| MsG0780041226.01.T01 | MTR_8g035520 | 37.195 | 164 | 100 | 3 | 4 | 165 | 21 | 183 | 3.55e-31 | 115 |
| MsG0780041226.01.T01 | MTR_1g102160 | 38.462 | 169 | 94 | 3 | 4 | 169 | 19 | 180 | 4.27e-31 | 115 |
| MsG0780041226.01.T01 | MTR_1g012430 | 38.415 | 164 | 91 | 5 | 3 | 165 | 10 | 164 | 8.13e-31 | 114 |
| MsG0780041226.01.T01 | MTR_7g406880 | 36.571 | 175 | 96 | 5 | 2 | 167 | 27 | 195 | 1.53e-30 | 114 |
| MsG0780041226.01.T01 | MTR_1g111280 | 37.356 | 174 | 92 | 5 | 2 | 167 | 32 | 196 | 4.01e-30 | 112 |
| MsG0780041226.01.T01 | MTR_6g464120 | 38.554 | 166 | 94 | 4 | 4 | 167 | 24 | 183 | 4.28e-30 | 115 |
| MsG0780041226.01.T01 | MTR_1g102170 | 39.375 | 160 | 85 | 4 | 9 | 165 | 43 | 193 | 1.07e-29 | 111 |
| MsG0780041226.01.T01 | MTR_7g056207 | 36.310 | 168 | 102 | 3 | 3 | 167 | 47 | 212 | 2.30e-29 | 110 |
| MsG0780041226.01.T01 | MTR_5g005900 | 35.673 | 171 | 102 | 4 | 3 | 167 | 16 | 184 | 5.12e-29 | 109 |
| MsG0780041226.01.T01 | MTR_1g105020 | 37.278 | 169 | 99 | 6 | 3 | 167 | 15 | 180 | 7.79e-28 | 106 |
| MsG0780041226.01.T01 | MTR_3g012440 | 34.239 | 184 | 100 | 6 | 2 | 167 | 13 | 193 | 1.50e-27 | 105 |
| MsG0780041226.01.T01 | MTR_6g464280 | 43.972 | 141 | 68 | 4 | 29 | 165 | 45 | 178 | 1.54e-27 | 105 |
| MsG0780041226.01.T01 | MTR_4g086480 | 34.545 | 165 | 105 | 3 | 4 | 167 | 20 | 182 | 6.50e-27 | 103 |
| MsG0780041226.01.T01 | MTR_4g086510 | 35.119 | 168 | 104 | 3 | 3 | 167 | 15 | 180 | 6.77e-27 | 103 |
| MsG0780041226.01.T01 | MTR_0007s0520 | 38.272 | 162 | 84 | 4 | 9 | 165 | 26 | 176 | 1.02e-26 | 103 |
| MsG0780041226.01.T01 | MTR_5g005920 | 35.673 | 171 | 104 | 4 | 4 | 169 | 21 | 190 | 1.09e-25 | 100 |
| MsG0780041226.01.T01 | MTR_6g464320 | 40.714 | 140 | 72 | 4 | 30 | 165 | 47 | 179 | 1.09e-25 | 100 |
| MsG0780041226.01.T01 | MTR_3g450680 | 34.483 | 174 | 104 | 5 | 3 | 169 | 23 | 193 | 2.96e-25 | 99.8 |
| MsG0780041226.01.T01 | MTR_4g086470 | 34.337 | 166 | 106 | 3 | 3 | 167 | 18 | 181 | 5.64e-25 | 98.6 |
| MsG0780041226.01.T01 | MTR_6g464510 | 35.185 | 162 | 94 | 4 | 8 | 165 | 27 | 181 | 5.68e-25 | 99.0 |
| MsG0780041226.01.T01 | MTR_8g040860 | 33.728 | 169 | 107 | 4 | 4 | 169 | 24 | 190 | 1.10e-24 | 98.2 |
| MsG0780041226.01.T01 | MTR_4g086540 | 36.646 | 161 | 91 | 5 | 7 | 165 | 29 | 180 | 1.34e-24 | 97.8 |
| MsG0780041226.01.T01 | MTR_7g051020 | 35.714 | 168 | 106 | 2 | 3 | 169 | 19 | 185 | 3.96e-21 | 88.6 |
| MsG0780041226.01.T01 | MTR_6g065400 | 33.562 | 146 | 81 | 2 | 28 | 165 | 55 | 192 | 1.19e-20 | 87.4 |
| MsG0780041226.01.T01 | MTR_5g005850 | 31.111 | 180 | 106 | 6 | 1 | 169 | 18 | 190 | 7.70e-20 | 85.1 |
| MsG0780041226.01.T01 | MTR_1g104930 | 35.714 | 168 | 103 | 3 | 3 | 169 | 19 | 182 | 1.22e-19 | 84.3 |
| MsG0780041226.01.T01 | MTR_6g065110 | 32.192 | 146 | 83 | 2 | 28 | 165 | 56 | 193 | 1.59e-19 | 84.3 |
| MsG0780041226.01.T01 | MTR_6g065270 | 32.667 | 150 | 81 | 2 | 28 | 165 | 53 | 194 | 5.60e-19 | 82.8 |
| MsG0780041226.01.T01 | MTR_6g065370 | 31.507 | 146 | 84 | 2 | 28 | 165 | 55 | 192 | 5.80e-19 | 82.8 |
| MsG0780041226.01.T01 | MTR_6g065120 | 31.126 | 151 | 87 | 2 | 24 | 165 | 52 | 194 | 1.39e-18 | 81.6 |
| MsG0780041226.01.T01 | MTR_6g065340 | 30.137 | 146 | 86 | 2 | 28 | 165 | 66 | 203 | 1.96e-18 | 81.3 |
| MsG0780041226.01.T01 | MTR_1g104933 | 36.095 | 169 | 101 | 5 | 3 | 169 | 17 | 180 | 2.07e-18 | 80.9 |
| MsG0780041226.01.T01 | MTR_1g082210 | 32.639 | 144 | 77 | 3 | 34 | 165 | 67 | 202 | 2.31e-18 | 81.3 |
| MsG0780041226.01.T01 | MTR_6g065430 | 32.867 | 143 | 87 | 2 | 28 | 169 | 56 | 190 | 3.48e-18 | 80.5 |
| MsG0780041226.01.T01 | MTR_8g035500 | 28.161 | 174 | 109 | 5 | 4 | 167 | 12 | 179 | 1.19e-17 | 79.0 |
| MsG0780041226.01.T01 | MTR_1g089310 | 37.736 | 106 | 58 | 1 | 60 | 165 | 121 | 218 | 6.35e-17 | 77.4 |
| MsG0780041226.01.T01 | MTR_1g089310 | 37.736 | 106 | 58 | 1 | 60 | 165 | 105 | 202 | 6.67e-17 | 77.0 |
| MsG0780041226.01.T01 | MTR_7g093950 | 36.792 | 106 | 59 | 1 | 60 | 165 | 106 | 203 | 2.35e-16 | 75.5 |
| MsG0780041226.01.T01 | MTR_6g022710 | 32.414 | 145 | 68 | 5 | 48 | 163 | 146 | 289 | 1.64e-11 | 62.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780041226.01.T01 | AT5G06570 | 58.235 | 170 | 67 | 2 | 1 | 167 | 30 | 198 | 1.42e-54 | 176 |
| MsG0780041226.01.T01 | AT5G06570 | 58.235 | 170 | 67 | 2 | 1 | 167 | 30 | 198 | 1.42e-54 | 176 |
| MsG0780041226.01.T01 | AT5G06570 | 58.235 | 170 | 67 | 2 | 1 | 167 | 15 | 183 | 1.68e-54 | 176 |
| MsG0780041226.01.T01 | AT5G06570 | 58.235 | 170 | 67 | 2 | 1 | 167 | 15 | 183 | 1.68e-54 | 176 |
| MsG0780041226.01.T01 | AT2G45600 | 41.212 | 165 | 87 | 5 | 4 | 165 | 16 | 173 | 1.13e-34 | 124 |
| MsG0780041226.01.T01 | AT5G16080 | 42.012 | 169 | 89 | 5 | 2 | 166 | 34 | 197 | 1.74e-34 | 124 |
| MsG0780041226.01.T01 | AT1G68620 | 39.286 | 168 | 94 | 3 | 2 | 167 | 29 | 190 | 2.05e-32 | 118 |
| MsG0780041226.01.T01 | AT5G62180 | 41.212 | 165 | 82 | 5 | 9 | 165 | 21 | 178 | 3.55e-30 | 112 |
| MsG0780041226.01.T01 | AT2G45610 | 39.706 | 136 | 78 | 2 | 33 | 165 | 49 | 183 | 4.81e-28 | 107 |
| MsG0780041226.01.T01 | AT1G49640 | 33.333 | 171 | 103 | 5 | 1 | 167 | 11 | 174 | 5.11e-28 | 106 |
| MsG0780041226.01.T01 | AT1G19190 | 33.939 | 165 | 104 | 4 | 4 | 165 | 13 | 175 | 3.06e-27 | 104 |
| MsG0780041226.01.T01 | AT1G47480 | 33.133 | 166 | 102 | 4 | 1 | 165 | 13 | 170 | 7.08e-26 | 100 |
| MsG0780041226.01.T01 | AT5G23530 | 39.130 | 161 | 84 | 5 | 9 | 165 | 35 | 185 | 1.26e-25 | 100 |
| MsG0780041226.01.T01 | AT1G49650 | 35.294 | 170 | 101 | 4 | 3 | 167 | 68 | 233 | 5.74e-25 | 99.8 |
| MsG0780041226.01.T01 | AT3G48690 | 34.545 | 165 | 104 | 4 | 3 | 165 | 12 | 174 | 4.25e-24 | 96.7 |
| MsG0780041226.01.T01 | AT2G03550 | 34.132 | 167 | 99 | 5 | 3 | 165 | 12 | 171 | 5.05e-24 | 96.3 |
| MsG0780041226.01.T01 | AT1G49660 | 33.918 | 171 | 106 | 4 | 1 | 167 | 10 | 177 | 1.40e-23 | 95.1 |
| MsG0780041226.01.T01 | AT3G48700 | 33.140 | 172 | 108 | 4 | 3 | 169 | 12 | 181 | 1.51e-23 | 95.1 |
| MsG0780041226.01.T01 | AT5G27320 | 34.899 | 149 | 78 | 3 | 28 | 165 | 61 | 201 | 5.09e-19 | 83.2 |
| MsG0780041226.01.T01 | AT3G05120 | 32.653 | 147 | 78 | 2 | 34 | 167 | 67 | 205 | 3.26e-18 | 80.9 |
| MsG0780041226.01.T01 | AT3G63010 | 33.099 | 142 | 73 | 2 | 38 | 165 | 70 | 203 | 3.85e-17 | 77.8 |
| MsG0780041226.01.T01 | AT5G14310 | 30.872 | 149 | 70 | 4 | 48 | 165 | 140 | 286 | 1.14e-11 | 62.8 |
| MsG0780041226.01.T01 | AT3G27320 | 29.139 | 151 | 75 | 3 | 46 | 165 | 120 | 269 | 2.92e-11 | 61.2 |
| MsG0780041226.01.T01 | AT3G27320 | 29.139 | 151 | 75 | 3 | 46 | 165 | 152 | 301 | 3.26e-11 | 61.2 |
Find 55 sgRNAs with CRISPR-Local
Find 56 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCATACAACAATTGTGAATA+TGG | 0.214269 | 7:+88458166 | None:intergenic |
| CTTTGACAGTGTGTTTATTA+TGG | 0.244190 | 7:-88457974 | MsG0780041226.01.T01:CDS |
| GAAGTTGTTAGAACGGTAAA+TGG | 0.252525 | 7:+88458371 | None:intergenic |
| CCAGTGATTGTGTTCCTTCA+TGG | 0.273519 | 7:-88458222 | MsG0780041226.01.T01:CDS |
| TTTATTATGGGTGACTCTTC+TGG | 0.275927 | 7:-88457961 | MsG0780041226.01.T01:CDS |
| CACCAGCTCGCGGTTCGGTT+TGG | 0.326565 | 7:-88457925 | MsG0780041226.01.T01:CDS |
| TTCTTGTTGTTGATGTTGTT+TGG | 0.341111 | 7:+88458255 | None:intergenic |
| GGTGAGGTGGCTTCAAAGGC+AGG | 0.367234 | 7:-88458046 | MsG0780041226.01.T01:CDS |
| TTTGACAGTGTGTTTATTAT+GGG | 0.368917 | 7:-88457973 | MsG0780041226.01.T01:CDS |
| ACTGAGTTGTCTTCTATTGT+GGG | 0.382901 | 7:+88458336 | None:intergenic |
| TTTGTTTCGGCTCCCGTACA+TGG | 0.389708 | 7:-88458191 | MsG0780041226.01.T01:CDS |
| CAGGGGTTGAGCCATGGTTA+TGG | 0.420795 | 7:-88458027 | MsG0780041226.01.T01:CDS |
| CATACAACAATTGTGAATAT+GGG | 0.426547 | 7:+88458167 | None:intergenic |
| TACTGAGTTGTCTTCTATTG+TGG | 0.438893 | 7:+88458335 | None:intergenic |
| GTGAATATGGGGCCATGTAC+GGG | 0.442379 | 7:+88458179 | None:intergenic |
| GTCGAAAAGACAGTCTTTGA+AGG | 0.443728 | 7:+88458311 | None:intergenic |
| TGTGAATATGGGGCCATGTA+CGG | 0.460692 | 7:+88458178 | None:intergenic |
| TTGCTCACCAGCTCGCGGTT+CGG | 0.462960 | 7:-88457930 | MsG0780041226.01.T01:CDS |
| GAAATTTGAAGTTGTTAGAA+CGG | 0.477889 | 7:+88458364 | None:intergenic |
| TCTGGTGCAAGCCGGTAGTC+CGG | 0.481494 | 7:+88458108 | None:intergenic |
| CGGTTTGGATTTGATATCAG+TGG | 0.491483 | 7:-88457910 | MsG0780041226.01.T01:CDS |
| AACCGTCGCTATAGAGTTGA+AGG | 0.495505 | 7:+88458394 | None:intergenic |
| GCAGCTGGAAGACGGTGCTC+TGG | 0.503385 | 7:+88458090 | None:intergenic |
| AAGCGGTGAGGTGGCTTCAA+AGG | 0.511531 | 7:-88458050 | MsG0780041226.01.T01:CDS |
| TGGTGGTTGCACCGGACTAC+CGG | 0.511979 | 7:-88458119 | MsG0780041226.01.T01:CDS |
| TCAAGCACTGGTGGTTGCAC+CGG | 0.513886 | 7:-88458127 | MsG0780041226.01.T01:CDS |
| CCATGAAGGAACACAATCAC+TGG | 0.524194 | 7:+88458222 | None:intergenic |
| GTGAGGTGGCTTCAAAGGCA+GGG | 0.524997 | 7:-88458045 | MsG0780041226.01.T01:CDS |
| ACAGCATCATCAATTGCAGC+TGG | 0.532717 | 7:+88458075 | None:intergenic |
| ATTGTGTTCCTTCATGGTGG+AGG | 0.533059 | 7:-88458216 | MsG0780041226.01.T01:CDS |
| GACGGTGCTCTGGTGCAAGC+CGG | 0.547439 | 7:+88458100 | None:intergenic |
| TTCCTTCAACTCTATAGCGA+CGG | 0.551743 | 7:-88458396 | MsG0780041226.01.T01:CDS |
| GGTTTGGATTTGATATCAGT+GGG | 0.552497 | 7:-88457909 | MsG0780041226.01.T01:CDS |
| CGCTGGACTTCAAGCACTGG+TGG | 0.553404 | 7:-88458136 | MsG0780041226.01.T01:CDS |
| ATCCAAACCGAACCGCGAGC+TGG | 0.554512 | 7:+88457923 | None:intergenic |
| CAAAGGCAGGGGTTGAGCCA+TGG | 0.559944 | 7:-88458033 | MsG0780041226.01.T01:CDS |
| TGAGGTGGCTTCAAAGGCAG+GGG | 0.564606 | 7:-88458044 | MsG0780041226.01.T01:CDS |
| CATCAATTGCAGCTGGAAGA+CGG | 0.570423 | 7:+88458082 | None:intergenic |
| AACAAAAGCCTCCACCATGA+AGG | 0.573822 | 7:+88458208 | None:intergenic |
| TTATGGGTGACTCTTCTGGC+GGG | 0.581327 | 7:-88457957 | MsG0780041226.01.T01:CDS |
| TGATGTTGTTTGGTTTGTAG+AGG | 0.582952 | 7:+88458265 | None:intergenic |
| AGCCGCTGGACTTCAAGCAC+TGG | 0.585205 | 7:-88458139 | MsG0780041226.01.T01:CDS |
| ACTGTCAAAGTCAACATCAC+TGG | 0.588550 | 7:+88457987 | None:intergenic |
| TGTTGTATGAGTCTAGCCGC+TGG | 0.594495 | 7:-88458153 | MsG0780041226.01.T01:CDS |
| ATTATGGGTGACTCTTCTGG+CGG | 0.606698 | 7:-88457958 | MsG0780041226.01.T01:CDS |
| ATACAACAATTGTGAATATG+GGG | 0.617658 | 7:+88458168 | None:intergenic |
| ATGCTGTCGAAGCGGTGAGG+TGG | 0.618657 | 7:-88458059 | MsG0780041226.01.T01:CDS |
| CACCAGTGCTTGAAGTCCAG+CGG | 0.635664 | 7:+88458137 | None:intergenic |
| ACATCACTGGTCGTCAACCA+TGG | 0.636649 | 7:+88458000 | None:intergenic |
| GCCATGGTTATGGAGAGCCA+TGG | 0.645828 | 7:-88458017 | MsG0780041226.01.T01:CDS |
| GTGATTGTGTTCCTTCATGG+TGG | 0.647455 | 7:-88458219 | MsG0780041226.01.T01:CDS |
| GAATATTGCTCACCAGCTCG+CGG | 0.656723 | 7:-88457935 | MsG0780041226.01.T01:CDS |
| ACCATGGCTCTCCATAACCA+TGG | 0.673171 | 7:+88458016 | None:intergenic |
| AATTGATGATGCTGTCGAAG+CGG | 0.713811 | 7:-88458067 | MsG0780041226.01.T01:CDS |
| ATGATGCTGTCGAAGCGGTG+AGG | 0.739300 | 7:-88458062 | MsG0780041226.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | ATACAACAATTGTGAATATG+GGG | + | Chr7:88458131-88458150 | None:intergenic | 25.0% |
| ! | CATACAACAATTGTGAATAT+GGG | + | Chr7:88458132-88458151 | None:intergenic | 25.0% |
| ! | TCATACAACAATTGTGAATA+TGG | + | Chr7:88458133-88458152 | None:intergenic | 25.0% |
| ! | TTTGACAGTGTGTTTATTAT+GGG | - | Chr7:88458323-88458342 | MsG0780041226.01.T01:CDS | 25.0% |
| !! | GAAATTTGAAGTTGTTAGAA+CGG | + | Chr7:88457935-88457954 | None:intergenic | 25.0% |
| CTTTGACAGTGTGTTTATTA+TGG | - | Chr7:88458322-88458341 | MsG0780041226.01.T01:CDS | 30.0% | |
| !! | TTCTTGTTGTTGATGTTGTT+TGG | + | Chr7:88458044-88458063 | None:intergenic | 30.0% |
| ACTGAGTTGTCTTCTATTGT+GGG | + | Chr7:88457963-88457982 | None:intergenic | 35.0% | |
| GAAGTTGTTAGAACGGTAAA+TGG | + | Chr7:88457928-88457947 | None:intergenic | 35.0% | |
| GGTTTGGATTTGATATCAGT+GGG | - | Chr7:88458387-88458406 | MsG0780041226.01.T01:CDS | 35.0% | |
| TACTGAGTTGTCTTCTATTG+TGG | + | Chr7:88457964-88457983 | None:intergenic | 35.0% | |
| TTTATTATGGGTGACTCTTC+TGG | - | Chr7:88458335-88458354 | MsG0780041226.01.T01:CDS | 35.0% | |
| !! | TGATGTTGTTTGGTTTGTAG+AGG | + | Chr7:88458034-88458053 | None:intergenic | 35.0% |
| ACTGTCAAAGTCAACATCAC+TGG | + | Chr7:88458312-88458331 | None:intergenic | 40.0% | |
| GTCGAAAAGACAGTCTTTGA+AGG | + | Chr7:88457988-88458007 | None:intergenic | 40.0% | |
| TTCCTTCAACTCTATAGCGA+CGG | - | Chr7:88457900-88457919 | MsG0780041226.01.T01:CDS | 40.0% | |
| ! | CGGTTTGGATTTGATATCAG+TGG | - | Chr7:88458386-88458405 | MsG0780041226.01.T01:CDS | 40.0% |
| !! | AATTGATGATGCTGTCGAAG+CGG | - | Chr7:88458229-88458248 | MsG0780041226.01.T01:CDS | 40.0% |
| AACAAAAGCCTCCACCATGA+AGG | + | Chr7:88458091-88458110 | None:intergenic | 45.0% | |
| AACCGTCGCTATAGAGTTGA+AGG | + | Chr7:88457905-88457924 | None:intergenic | 45.0% | |
| ACAGCATCATCAATTGCAGC+TGG | + | Chr7:88458224-88458243 | None:intergenic | 45.0% | |
| ATTATGGGTGACTCTTCTGG+CGG | - | Chr7:88458338-88458357 | MsG0780041226.01.T01:CDS | 45.0% | |
| ATTGTGTTCCTTCATGGTGG+AGG | - | Chr7:88458080-88458099 | MsG0780041226.01.T01:CDS | 45.0% | |
| CATCAATTGCAGCTGGAAGA+CGG | + | Chr7:88458217-88458236 | None:intergenic | 45.0% | |
| CCAGTGATTGTGTTCCTTCA+TGG | - | Chr7:88458074-88458093 | MsG0780041226.01.T01:CDS | 45.0% | |
| CCATGAAGGAACACAATCAC+TGG | + | Chr7:88458077-88458096 | None:intergenic | 45.0% | |
| GTGATTGTGTTCCTTCATGG+TGG | - | Chr7:88458077-88458096 | MsG0780041226.01.T01:CDS | 45.0% | |
| TGTGAATATGGGGCCATGTA+CGG | + | Chr7:88458121-88458140 | None:intergenic | 45.0% | |
| !! | CATGGTGGAGGCTTTTGTTT+CGG | - | Chr7:88458092-88458111 | MsG0780041226.01.T01:CDS | 45.0% |
| ACATCACTGGTCGTCAACCA+TGG | + | Chr7:88458299-88458318 | None:intergenic | 50.0% | |
| ACCATGGCTCTCCATAACCA+TGG | + | Chr7:88458283-88458302 | None:intergenic | 50.0% | |
| GAATATTGCTCACCAGCTCG+CGG | - | Chr7:88458361-88458380 | MsG0780041226.01.T01:CDS | 50.0% | |
| GTGAATATGGGGCCATGTAC+GGG | + | Chr7:88458120-88458139 | None:intergenic | 50.0% | |
| TGTTGTATGAGTCTAGCCGC+TGG | - | Chr7:88458143-88458162 | MsG0780041226.01.T01:CDS | 50.0% | |
| TTATGGGTGACTCTTCTGGC+GGG | - | Chr7:88458339-88458358 | MsG0780041226.01.T01:CDS | 50.0% | |
| TTTGTTTCGGCTCCCGTACA+TGG | - | Chr7:88458105-88458124 | MsG0780041226.01.T01:CDS | 50.0% | |
| AAGCGGTGAGGTGGCTTCAA+AGG | - | Chr7:88458246-88458265 | MsG0780041226.01.T01:CDS | 55.0% | |
| ATGATGCTGTCGAAGCGGTG+AGG | - | Chr7:88458234-88458253 | MsG0780041226.01.T01:CDS | 55.0% | |
| CACCAGTGCTTGAAGTCCAG+CGG | + | Chr7:88458162-88458181 | None:intergenic | 55.0% | |
| CAGGGGTTGAGCCATGGTTA+TGG | - | Chr7:88458269-88458288 | MsG0780041226.01.T01:CDS | 55.0% | |
| GCCATGGTTATGGAGAGCCA+TGG | - | Chr7:88458279-88458298 | MsG0780041226.01.T01:CDS | 55.0% | |
| GTGAGGTGGCTTCAAAGGCA+GGG | - | Chr7:88458251-88458270 | MsG0780041226.01.T01:CDS | 55.0% | |
| TGAGGTGGCTTCAAAGGCAG+GGG | - | Chr7:88458252-88458271 | MsG0780041226.01.T01:CDS | 55.0% | |
| !! | TCAAGCACTGGTGGTTGCAC+CGG | - | Chr7:88458169-88458188 | MsG0780041226.01.T01:CDS | 55.0% |
| AGCCGCTGGACTTCAAGCAC+TGG | - | Chr7:88458157-88458176 | MsG0780041226.01.T01:CDS | 60.0% | |
| ATCCAAACCGAACCGCGAGC+TGG | + | Chr7:88458376-88458395 | None:intergenic | 60.0% | |
| ATGCTGTCGAAGCGGTGAGG+TGG | - | Chr7:88458237-88458256 | MsG0780041226.01.T01:CDS | 60.0% | |
| CAAAGGCAGGGGTTGAGCCA+TGG | - | Chr7:88458263-88458282 | MsG0780041226.01.T01:CDS | 60.0% | |
| GGTGAGGTGGCTTCAAAGGC+AGG | - | Chr7:88458250-88458269 | MsG0780041226.01.T01:CDS | 60.0% | |
| TCTGGTGCAAGCCGGTAGTC+CGG | + | Chr7:88458191-88458210 | None:intergenic | 60.0% | |
| TGGTGGTTGCACCGGACTAC+CGG | - | Chr7:88458177-88458196 | MsG0780041226.01.T01:CDS | 60.0% | |
| TTGCTCACCAGCTCGCGGTT+CGG | - | Chr7:88458366-88458385 | MsG0780041226.01.T01:CDS | 60.0% | |
| !! | CGCTGGACTTCAAGCACTGG+TGG | - | Chr7:88458160-88458179 | MsG0780041226.01.T01:CDS | 60.0% |
| GACGGTGCTCTGGTGCAAGC+CGG | + | Chr7:88458199-88458218 | None:intergenic | 65.0% | |
| GCAGCTGGAAGACGGTGCTC+TGG | + | Chr7:88458209-88458228 | None:intergenic | 65.0% | |
| !! | CACCAGCTCGCGGTTCGGTT+TGG | - | Chr7:88458371-88458390 | MsG0780041226.01.T01:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr7 | gene | 88457894 | 88458424 | 88457894 | ID=MsG0780041226.01;Name=MsG0780041226.01 |
| Chr7 | mRNA | 88457894 | 88458424 | 88457894 | ID=MsG0780041226.01.T01;Parent=MsG0780041226.01;Name=MsG0780041226.01.T01;_AED=0.45;_eAED=0.45;_QI=0|-1|0|1|-1|1|1|0|176 |
| Chr7 | exon | 88457894 | 88458424 | 88457894 | ID=MsG0780041226.01.T01:exon:41521;Parent=MsG0780041226.01.T01 |
| Chr7 | CDS | 88457894 | 88458424 | 88457894 | ID=MsG0780041226.01.T01:cds;Parent=MsG0780041226.01.T01 |
| Gene Sequence |
| Protein sequence |
