AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0880043429.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880043429.01.T01 MTR_8g035540 95.732 328 14 0 1 328 1 328 0.0 641
MsG0880043429.01.T01 MTR_8g035520 76.415 318 71 1 1 318 13 326 3.43e-176 491
MsG0880043429.01.T01 MTR_6g464510 48.447 322 150 5 1 316 15 326 1.57e-105 311
MsG0880043429.01.T01 MTR_6g464280 48.148 324 148 6 2 316 12 324 3.82e-102 303
MsG0880043429.01.T01 MTR_1g102160 47.546 326 154 7 1 318 11 327 2.05e-94 283
MsG0880043429.01.T01 MTR_6g464320 46.296 324 154 6 2 316 13 325 8.77e-94 282
MsG0880043429.01.T01 MTR_1g102170 46.875 320 149 6 1 313 30 335 2.15e-93 281
MsG0880043429.01.T01 MTR_8g035500 41.693 319 180 4 1 317 4 318 4.42e-92 277
MsG0880043429.01.T01 MTR_6g464120 46.302 311 155 5 2 309 17 318 9.87e-89 275
MsG0880043429.01.T01 MTR_0007s0520 44.937 316 154 6 9 316 21 324 5.57e-87 265
MsG0880043429.01.T01 MTR_1g111280 35.404 322 181 9 9 316 34 342 1.75e-53 179
MsG0880043429.01.T01 MTR_7g406880 33.028 327 194 8 9 316 29 349 1.25e-52 177
MsG0880043429.01.T01 MTR_6g065370 34.122 296 173 6 32 316 49 333 2.13e-48 166
MsG0880043429.01.T01 MTR_6g065400 34.459 296 172 6 32 316 49 333 3.15e-48 165
MsG0880043429.01.T01 MTR_6g065430 33.439 314 189 6 14 320 31 331 2.14e-47 163
MsG0880043429.01.T01 MTR_4g050140 34.528 307 188 6 14 316 18 315 5.63e-47 161
MsG0880043429.01.T01 MTR_6g065110 32.441 299 178 6 35 321 53 339 7.35e-46 159
MsG0880043429.01.T01 MTR_6g065120 34.007 297 173 6 32 316 50 335 2.10e-45 158
MsG0880043429.01.T01 MTR_6g065270 33.663 303 169 7 32 316 47 335 3.41e-45 157
MsG0880043429.01.T01 MTR_7g107040 33.540 322 186 9 9 319 15 319 9.92e-45 155
MsG0880043429.01.T01 MTR_4g086510 34.494 316 185 9 9 316 16 317 5.71e-44 153
MsG0880043429.01.T01 MTR_1g012430 36.515 241 136 8 5 244 9 233 2.34e-43 152
MsG0880043429.01.T01 MTR_3g101420 32.381 315 191 10 9 316 15 314 1.33e-42 150
MsG0880043429.01.T01 MTR_0007s0480 44.970 169 84 3 156 316 3 170 4.93e-42 144
MsG0880043429.01.T01 MTR_7g056207 32.628 331 185 9 9 323 48 356 5.11e-42 149
MsG0880043429.01.T01 MTR_3g012440 40.957 188 109 2 56 243 74 259 6.19e-40 143
MsG0880043429.01.T01 MTR_1g082210 29.630 324 198 8 13 321 37 345 1.11e-37 137
MsG0880043429.01.T01 MTR_4g086480 32.812 320 188 10 9 319 20 321 2.70e-37 136
MsG0880043429.01.T01 MTR_5g005920 31.190 311 193 10 14 316 26 323 2.51e-36 134
MsG0880043429.01.T01 MTR_1g089310 39.888 178 95 3 64 240 98 264 5.46e-36 133
MsG0880043429.01.T01 MTR_1g089310 39.888 178 95 3 64 240 114 280 5.60e-36 133
MsG0880043429.01.T01 MTR_6g065340 29.874 318 176 9 14 316 43 328 7.04e-36 132
MsG0880043429.01.T01 MTR_7g093950 32.061 262 155 6 64 316 99 346 4.96e-35 130
MsG0880043429.01.T01 MTR_4g086540 35.263 190 111 3 14 203 31 208 6.34e-35 129
MsG0880043429.01.T01 MTR_3g450680 31.385 325 189 12 9 316 24 331 6.19e-33 124
MsG0880043429.01.T01 MTR_4g086470 29.712 313 173 9 26 319 36 320 1.17e-32 124
MsG0880043429.01.T01 MTR_1g105020 30.915 317 195 12 9 315 16 318 1.71e-32 123
MsG0880043429.01.T01 MTR_5g005850 34.498 229 134 6 9 231 21 239 7.71e-31 119
MsG0880043429.01.T01 MTR_1g104930 41.579 190 100 4 9 196 20 200 2.88e-30 117
MsG0880043429.01.T01 MTR_5g005900 36.410 195 114 5 9 198 17 206 2.99e-29 114
MsG0880043429.01.T01 MTR_7g051020 40.526 190 105 4 9 196 20 203 2.01e-28 112
MsG0880043429.01.T01 MTR_8g040860 35.678 199 121 4 9 205 24 217 6.61e-27 108
MsG0880043429.01.T01 MTR_6g022710 27.526 287 172 5 70 322 159 443 2.39e-26 108
MsG0880043429.01.T01 MTR_1g104933 41.401 157 86 3 42 196 46 198 6.78e-24 100
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880043429.01.T01 AT2G45600 52.870 331 138 5 2 327 9 326 4.95e-122 354
MsG0880043429.01.T01 AT2G45610 46.226 318 166 3 1 316 9 323 5.39e-101 300
MsG0880043429.01.T01 AT5G62180 48.012 327 154 7 2 320 9 327 1.01e-96 289
MsG0880043429.01.T01 AT5G16080 36.120 299 176 6 9 303 36 323 5.71e-52 175
MsG0880043429.01.T01 AT1G68620 39.918 243 132 5 1 240 24 255 3.38e-51 173
MsG0880043429.01.T01 AT5G06570 35.065 308 185 8 9 308 18 318 2.76e-38 139
MsG0880043429.01.T01 AT5G06570 35.065 308 185 8 9 308 18 318 2.76e-38 139
MsG0880043429.01.T01 AT5G06570 35.065 308 185 8 9 308 33 333 3.39e-38 139
MsG0880043429.01.T01 AT5G06570 35.065 308 185 8 9 308 33 333 3.39e-38 139
MsG0880043429.01.T01 AT5G23530 30.696 316 194 8 13 321 34 331 1.86e-37 137
MsG0880043429.01.T01 AT1G49640 36.979 192 108 4 9 198 14 194 4.36e-37 135
MsG0880043429.01.T01 AT3G63010 29.674 337 204 8 8 327 32 352 3.06e-35 131
MsG0880043429.01.T01 AT3G48690 32.895 228 141 5 9 232 13 232 4.57e-35 130
MsG0880043429.01.T01 AT5G27320 28.840 319 196 8 13 316 37 339 4.62e-35 130
MsG0880043429.01.T01 AT1G47480 34.348 230 134 6 11 240 18 230 1.36e-33 126
MsG0880043429.01.T01 AT1G49650 36.126 191 115 4 9 198 69 253 1.97e-32 124
MsG0880043429.01.T01 AT3G48700 33.471 242 142 7 15 246 19 251 3.30e-32 122
MsG0880043429.01.T01 AT1G19190 32.420 219 137 5 8 220 12 225 6.41e-32 122
MsG0880043429.01.T01 AT1G49660 35.533 197 120 4 5 199 8 199 1.19e-31 121
MsG0880043429.01.T01 AT2G03550 37.968 187 108 3 10 196 14 192 2.10e-31 120
MsG0880043429.01.T01 AT3G05120 30.769 286 174 7 13 286 37 310 5.00e-31 120
MsG0880043429.01.T01 AT5G14310 32.031 256 133 6 29 245 106 359 1.56e-30 120
MsG0880043429.01.T01 AT3G27320 33.178 214 106 4 64 240 124 337 5.15e-28 113
MsG0880043429.01.T01 AT3G27320 33.178 214 106 4 64 240 156 369 7.21e-28 113

Find 109 sgRNAs with CRISPR-Local

Find 129 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGGCAACAATGGCAGGAATT+TGG 0.210540 8:+24671643 None:intergenic
AAAGGAGCTGGCGAAGATTT+TGG 0.238342 8:-24671133 MsG0880043429.01.T01:CDS
GAGGAAGACGGTGGAGGGTT+CGG 0.288881 8:+24671768 None:intergenic
TGAAATCTTTGATCATTCTA+AGG 0.290499 8:-24671055 MsG0880043429.01.T01:CDS
CACAAATTCGCCACCGCAAC+CGG 0.311175 8:-24671850 MsG0880043429.01.T01:CDS
AGCACGTAGACCCGTGAAAT+AGG 0.319272 8:+24671440 None:intergenic
GTAGAGAATGATGGGGAGTT+TGG 0.319640 8:+24671740 None:intergenic
AGGAAGACGGTGGAGGGTTC+GGG 0.325103 8:+24671769 None:intergenic
ACAACTTCTGATCAAGTATA+TGG 0.342181 8:-24671225 MsG0880043429.01.T01:CDS
GGCAACAATGGCAGGAATTT+GGG 0.345399 8:+24671644 None:intergenic
GCAGGGATGGTGGAAGATAA+GGG 0.378351 8:+24671680 None:intergenic
AGCAGGGATGGTGGAAGATA+AGG 0.379203 8:+24671679 None:intergenic
TAAGGGAGGAAGGGTGGTAG+AGG 0.393535 8:+24671697 None:intergenic
CGGTTGCGGTGGCGAATTTG+TGG 0.395908 8:+24671851 None:intergenic
GACTGAATCCGAGCTTCGTT+TGG 0.401433 8:-24671343 MsG0880043429.01.T01:CDS
ATGCAGTTGATTCTCTTCTC+TGG 0.401616 8:-24671569 MsG0880043429.01.T01:CDS
ATGATCCCTCGAATCTTAAC+GGG 0.416694 8:+24671399 None:intergenic
TCGAAGGATGGTGGCACGGT+GGG 0.424218 8:+24671876 None:intergenic
TATGATCCCTCGAATCTTAA+CGG 0.433679 8:+24671398 None:intergenic
CTTTGATAAATGTTTCTTGA+TGG 0.434816 8:-24671487 MsG0880043429.01.T01:CDS
ATACCCATCTTCATCAAAAC+AGG 0.438618 8:+24671086 None:intergenic
TTTCTTGTTAGTGAACCATC+AGG 0.441242 8:+24671909 None:intergenic
TATACTTGATCAGAAGTTGT+TGG 0.446383 8:+24671228 None:intergenic
TGGTCCCGGTCTTTGCCCTC+AGG 0.451278 8:+24671264 None:intergenic
GCAGCGGAGGAAGACGGTGG+AGG 0.452270 8:+24671762 None:intergenic
ATTCTCTACTTCCATGGTGG+TGG 0.458108 8:-24671726 MsG0880043429.01.T01:CDS
GGAAGATAAGGGAGGAAGGG+TGG 0.467019 8:+24671691 None:intergenic
TTTGATAAATGTTTCTTGAT+GGG 0.469837 8:-24671486 MsG0880043429.01.T01:CDS
TTGGGCAGCTAATGTGGAGC+AGG 0.470169 8:+24671662 None:intergenic
ATCGAAGGATGGTGGCACGG+TGG 0.483039 8:+24671875 None:intergenic
GCGGCTGGGAGGCGATGCTC+AGG 0.484399 8:+24671600 None:intergenic
CCATGGAAGTAGAGAATGAT+GGG 0.492333 8:+24671732 None:intergenic
ATGAGATCACCTGCTGGTAA+TGG 0.493022 8:+24671303 None:intergenic
ATCGTTAACCAAACGAAGCT+CGG 0.493769 8:+24671335 None:intergenic
CCCATCATTCTCTACTTCCA+TGG 0.494966 8:-24671732 MsG0880043429.01.T01:CDS
TCCATTGGTGGATAAGCAAA+AGG 0.501412 8:-24671151 MsG0880043429.01.T01:CDS
ACTGCATCATCATACGCGGC+TGG 0.502470 8:+24671585 None:intergenic
GATTGCAATACTCGTGGTCC+CGG 0.503398 8:+24671250 None:intergenic
TCAACGGAGGCAACAATGGC+AGG 0.505210 8:+24671636 None:intergenic
CAGCTAATGTGGAGCAGGGA+TGG 0.506859 8:+24671667 None:intergenic
ACCATGGAAGTAGAGAATGA+TGG 0.507797 8:+24671731 None:intergenic
GGTTGCGGTGGCGAATTTGT+GGG 0.510694 8:+24671852 None:intergenic
TGGTGGAAGATAAGGGAGGA+AGG 0.516872 8:+24671687 None:intergenic
AGGAATTTGGGCAGCTAATG+TGG 0.521371 8:+24671656 None:intergenic
CGTATATTGCCATTACCAGC+AGG 0.526449 8:-24671312 MsG0880043429.01.T01:CDS
CAAGTATATGGCGAAAAGAT+CGG 0.527967 8:-24671213 MsG0880043429.01.T01:CDS
ATCCAACGGAATCTGATCCA+TGG 0.529983 8:-24671527 MsG0880043429.01.T01:CDS
TGGCTACGCTGGAGATCCAT+TGG 0.537244 8:-24671166 MsG0880043429.01.T01:CDS
TCCACATGGTCTCTGATCCA+TGG 0.542444 8:+24671510 None:intergenic
ATCCATGGATCAGATTCCGT+TGG 0.545679 8:+24671525 None:intergenic
GTTTGCCTGAGGGCAAAGAC+CGG 0.546161 8:-24671269 MsG0880043429.01.T01:CDS
TTTGCCTGAGGGCAAAGACC+GGG 0.548229 8:-24671268 MsG0880043429.01.T01:CDS
TTCTTGATGGGAGACAGTGC+CGG 0.548245 8:-24671474 MsG0880043429.01.T01:CDS
GGCCGCACGCGGGGTGCACG+TGG 0.551288 8:-24671112 MsG0880043429.01.T01:CDS
CTGCATCATCATACGCGGCT+GGG 0.551693 8:+24671586 None:intergenic
GAAATAGGCTATATTACCTC+CGG 0.551917 8:+24671455 None:intergenic
AGGTAATATAGCCTATTTCA+CGG 0.552620 8:-24671451 MsG0880043429.01.T01:CDS
CAGCGGAGGAAGACGGTGGA+GGG 0.554188 8:+24671763 None:intergenic
CGAAGGATGGTGGCACGGTG+GGG 0.556375 8:+24671877 None:intergenic
TGGTCCCTGTGTTTGCCTGA+GGG 0.566573 8:-24671279 MsG0880043429.01.T01:CDS
GTGGTCCCTGTGTTTGCCTG+AGG 0.571411 8:-24671280 MsG0880043429.01.T01:CDS
GGTGGATAAGCAAAAGGAGC+TGG 0.572280 8:-24671145 MsG0880043429.01.T01:CDS
TGGCGAATTTGTGGGATCGA+AGG 0.576164 8:+24671860 None:intergenic
AGTTTGGCAGCGGAGGAAGA+CGG 0.578445 8:+24671756 None:intergenic
GAATTTGTGGGATCGAAGGA+TGG 0.578551 8:+24671864 None:intergenic
AAAATCAAACTAAACCCTGA+TGG 0.582070 8:-24671924 MsG0880043429.01.T01:CDS
GGTAATATAGCCTATTTCAC+GGG 0.582524 8:-24671450 MsG0880043429.01.T01:CDS
TTCTTGTTAGTGAACCATCA+GGG 0.584038 8:+24671910 None:intergenic
GGGATCGAAGGATGGTGGCA+CGG 0.586269 8:+24671872 None:intergenic
GGTGGAAGATAAGGGAGGAA+GGG 0.587951 8:+24671688 None:intergenic
GTTCGGGAGGAAGAGACGAA+TGG 0.588388 8:+24671785 None:intergenic
CTCCACGTGCACCCCGCGTG+CGG 0.589197 8:+24671110 None:intergenic
CTTTGCCCTCAGGCAAACAC+AGG 0.589918 8:+24671274 None:intergenic
TCTTTAGAGAGAGCCGGTTG+CGG 0.590395 8:+24671837 None:intergenic
TTAGAGAGAGCCGGTTGCGG+TGG 0.595065 8:+24671840 None:intergenic
CTAATGTGGAGCAGGGATGG+TGG 0.596208 8:+24671670 None:intergenic
AAAAGATCGGACGGTTGCCA+AGG 0.597107 8:-24671200 MsG0880043429.01.T01:CDS
AAGACGGTGGAGGGTTCGGG+AGG 0.598115 8:+24671772 None:intergenic
GATGGGGAGTTTGGCAGCGG+AGG 0.598370 8:+24671749 None:intergenic
CTACGCTGGAGATCCATTGG+TGG 0.598980 8:-24671163 MsG0880043429.01.T01:CDS
GGAATGTCTTTAGAGAGAGC+CGG 0.604854 8:+24671831 None:intergenic
ATAATCAACGGAGGCAACAA+TGG 0.605583 8:+24671632 None:intergenic
AATGATGGGGAGTTTGGCAG+CGG 0.606209 8:+24671746 None:intergenic
AAGTGTTACATAGAGGATTG+AGG 0.616571 8:+24670976 None:intergenic
ATCCCAAGCTCAAAATCCAA+CGG 0.621724 8:-24671541 MsG0880043429.01.T01:CDS
TACCAGCAGGTGATCTCATG+TGG 0.621833 8:-24671299 MsG0880043429.01.T01:CDS
CGGGAGGAAGAGACGAATGG+AGG 0.622270 8:+24671788 None:intergenic
ATCATTCTCTACTTCCATGG+TGG 0.627271 8:-24671729 MsG0880043429.01.T01:CDS
CATCATCATACGCGGCTGGG+AGG 0.634621 8:+24671589 None:intergenic
TCCCACGAAGTGTTACATAG+AGG 0.639443 8:+24670969 None:intergenic
TGGGCAGCTAATGTGGAGCA+GGG 0.644565 8:+24671663 None:intergenic
GACCACATGAGATCACCTGC+TGG 0.646204 8:+24671297 None:intergenic
TTGTTGGATTGCAATACTCG+TGG 0.647099 8:+24671244 None:intergenic
TTTGTGGGATCGAAGGATGG+TGG 0.649200 8:+24671867 None:intergenic
AACATTTATCAAAGTCCACA+TGG 0.649642 8:+24671496 None:intergenic
TTGGCAGCGGAGGAAGACGG+TGG 0.649761 8:+24671759 None:intergenic
TTGATGGGAGACAGTGCCGG+AGG 0.655276 8:-24671471 MsG0880043429.01.T01:CDS
GGGATGGTGGAAGATAAGGG+AGG 0.667136 8:+24671683 None:intergenic
AGAGGATGAAACCACCACCA+TGG 0.674528 8:+24671715 None:intergenic
TAGCCATTAACAAAACACCT+TGG 0.678425 8:+24671183 None:intergenic
TCCATGGATCAGAGACCATG+TGG 0.678870 8:-24671511 MsG0880043429.01.T01:CDS
AGGCGAGAGACGATAATCAA+CGG 0.682315 8:+24671620 None:intergenic
TATATGGCGAAAAGATCGGA+CGG 0.695072 8:-24671209 MsG0880043429.01.T01:CDS
TTTGCCCTCAGGCAAACACA+GGG 0.698468 8:+24671275 None:intergenic
ATCAACTGCATCATCATACG+CGG 0.698641 8:+24671581 None:intergenic
TGATCCCTCGAATCTTAACG+GGG 0.731170 8:+24671400 None:intergenic
GATCCCTCGAATCTTAACGG+GGG 0.742224 8:+24671401 None:intergenic
CATGGAAGTAGAGAATGATG+GGG 0.743084 8:+24671733 None:intergenic
CGAGAGACGATAATCAACGG+AGG 0.745311 8:+24671623 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTTGATAAATGTTTCTTGAT+GGG - Chr8:24671440-24671459 MsG0880043429.01.T01:CDS 20.0%
!!! ATGAAATATCCATTTTTTAG+TGG - Chr8:24671551-24671570 MsG0880043429.01.T01:CDS 20.0%
! CTTTGATAAATGTTTCTTGA+TGG - Chr8:24671439-24671458 MsG0880043429.01.T01:CDS 25.0%
! TGAAATCTTTGATCATTCTA+AGG - Chr8:24671871-24671890 MsG0880043429.01.T01:CDS 25.0%
!!! TTGATTTTGAGGAATTCATA+GGG + Chr8:24670990-24671009 None:intergenic 25.0%
!!! TTTGATTTTGAGGAATTCAT+AGG + Chr8:24670991-24671010 None:intergenic 25.0%
AAAATCAAACTAAACCCTGA+TGG - Chr8:24671002-24671021 MsG0880043429.01.T01:CDS 30.0%
AACATTTATCAAAGTCCACA+TGG + Chr8:24671433-24671452 None:intergenic 30.0%
ACAACTTCTGATCAAGTATA+TGG - Chr8:24671701-24671720 MsG0880043429.01.T01:CDS 30.0%
AGGTAATATAGCCTATTTCA+CGG - Chr8:24671475-24671494 MsG0880043429.01.T01:CDS 30.0%
TATACTTGATCAGAAGTTGT+TGG + Chr8:24671701-24671720 None:intergenic 30.0%
ATACCCATCTTCATCAAAAC+AGG + Chr8:24671843-24671862 None:intergenic 35.0%
CAAGTATATGGCGAAAAGAT+CGG - Chr8:24671713-24671732 MsG0880043429.01.T01:CDS 35.0%
CTCTGAACTCCACTAAAAAA+TGG + Chr8:24671563-24671582 None:intergenic 35.0%
GAAATAGGCTATATTACCTC+CGG + Chr8:24671474-24671493 None:intergenic 35.0%
GGTAATATAGCCTATTTCAC+GGG - Chr8:24671476-24671495 MsG0880043429.01.T01:CDS 35.0%
TAGCCATTAACAAAACACCT+TGG + Chr8:24671746-24671765 None:intergenic 35.0%
TATGATCCCTCGAATCTTAA+CGG + Chr8:24671531-24671550 None:intergenic 35.0%
TTCTTGTTAGTGAACCATCA+GGG + Chr8:24671019-24671038 None:intergenic 35.0%
TTTCTTGTTAGTGAACCATC+AGG + Chr8:24671020-24671039 None:intergenic 35.0%
!! TTGCCAAGGTGTTTTGTTAA+TGG - Chr8:24671740-24671759 MsG0880043429.01.T01:CDS 35.0%
!!! AGTCCTGTTTTGATGAAGAT+GGG - Chr8:24671837-24671856 MsG0880043429.01.T01:CDS 35.0%
!!! CAGGGTTTAGTTTGATTTTG+AGG + Chr8:24671001-24671020 None:intergenic 35.0%
!!! CATTTTTTAGTGGAGTTCAG+AGG - Chr8:24671561-24671580 MsG0880043429.01.T01:CDS 35.0%
ACCATGGAAGTAGAGAATGA+TGG + Chr8:24671198-24671217 None:intergenic 40.0%
ATAATCAACGGAGGCAACAA+TGG + Chr8:24671297-24671316 None:intergenic 40.0%
ATCAACTGCATCATCATACG+CGG + Chr8:24671348-24671367 None:intergenic 40.0%
ATCATTCTCTACTTCCATGG+TGG - Chr8:24671197-24671216 MsG0880043429.01.T01:CDS 40.0%
ATCCCAAGCTCAAAATCCAA+CGG - Chr8:24671385-24671404 MsG0880043429.01.T01:CDS 40.0%
ATCGTTAACCAAACGAAGCT+CGG + Chr8:24671594-24671613 None:intergenic 40.0%
ATGATCCCTCGAATCTTAAC+GGG + Chr8:24671530-24671549 None:intergenic 40.0%
CATGGAAGTAGAGAATGATG+GGG + Chr8:24671196-24671215 None:intergenic 40.0%
CCATGGAAGTAGAGAATGAT+GGG + Chr8:24671197-24671216 None:intergenic 40.0%
TATATGGCGAAAAGATCGGA+CGG - Chr8:24671717-24671736 MsG0880043429.01.T01:CDS 40.0%
TTGTTGGATTGCAATACTCG+TGG + Chr8:24671685-24671704 None:intergenic 40.0%
! ATGCAGTTGATTCTCTTCTC+TGG - Chr8:24671357-24671376 MsG0880043429.01.T01:CDS 40.0%
! ATTCCGTTGGATTTTGAGCT+TGG + Chr8:24671391-24671410 None:intergenic 40.0%
! TCCATTGGTGGATAAGCAAA+AGG - Chr8:24671775-24671794 MsG0880043429.01.T01:CDS 40.0%
! TCCTTTTGCTTATCCACCAA+TGG + Chr8:24671779-24671798 None:intergenic 40.0%
! TTCCGTTGGATTTTGAGCTT+GGG + Chr8:24671390-24671409 None:intergenic 40.0%
!! TGTTTTGTTAATGGCTACGC+TGG - Chr8:24671749-24671768 MsG0880043429.01.T01:CDS 40.0%
!!! GAGTCCTGTTTTGATGAAGA+TGG - Chr8:24671836-24671855 MsG0880043429.01.T01:CDS 40.0%
AGGAATTTGGGCAGCTAATG+TGG + Chr8:24671273-24671292 None:intergenic 45.0%
AGGCAACAATGGCAGGAATT+TGG + Chr8:24671286-24671305 None:intergenic 45.0%
AGGCGAGAGACGATAATCAA+CGG + Chr8:24671309-24671328 None:intergenic 45.0%
ATCCAACGGAATCTGATCCA+TGG - Chr8:24671399-24671418 MsG0880043429.01.T01:CDS 45.0%
ATCCATGGATCAGATTCCGT+TGG + Chr8:24671404-24671423 None:intergenic 45.0%
ATGAGATCACCTGCTGGTAA+TGG + Chr8:24671626-24671645 None:intergenic 45.0%
ATTCTCTACTTCCATGGTGG+TGG - Chr8:24671200-24671219 MsG0880043429.01.T01:CDS 45.0%
CCCATCATTCTCTACTTCCA+TGG - Chr8:24671194-24671213 MsG0880043429.01.T01:CDS 45.0%
CGTATATTGCCATTACCAGC+AGG - Chr8:24671614-24671633 MsG0880043429.01.T01:CDS 45.0%
GAATTTGTGGGATCGAAGGA+TGG + Chr8:24671065-24671084 None:intergenic 45.0%
GGCAACAATGGCAGGAATTT+GGG + Chr8:24671285-24671304 None:intergenic 45.0%
TGATCCCTCGAATCTTAACG+GGG + Chr8:24671529-24671548 None:intergenic 45.0%
! AAAGGAGCTGGCGAAGATTT+TGG - Chr8:24671793-24671812 MsG0880043429.01.T01:CDS 45.0%
! GGAATGTCTTTAGAGAGAGC+CGG + Chr8:24671098-24671117 None:intergenic 45.0%
! GTAGAGAATGATGGGGAGTT+TGG + Chr8:24671189-24671208 None:intergenic 45.0%
! GTTTTCGCAGCGGTGTTTAA+GGG + Chr8:24671119-24671138 None:intergenic 45.0%
AAAAGATCGGACGGTTGCCA+AGG - Chr8:24671726-24671745 MsG0880043429.01.T01:CDS 50.0%
AGAGGATGAAACCACCACCA+TGG + Chr8:24671214-24671233 None:intergenic 50.0%
AGCACGTAGACCCGTGAAAT+AGG + Chr8:24671489-24671508 None:intergenic 50.0%
CGAGAGACGATAATCAACGG+AGG + Chr8:24671306-24671325 None:intergenic 50.0%
GACTGAATCCGAGCTTCGTT+TGG - Chr8:24671583-24671602 MsG0880043429.01.T01:CDS 50.0%
GATCCCTCGAATCTTAACGG+GGG + Chr8:24671528-24671547 None:intergenic 50.0%
GATTGCAATACTCGTGGTCC+CGG + Chr8:24671679-24671698 None:intergenic 50.0%
GGTGGAAGATAAGGGAGGAA+GGG + Chr8:24671241-24671260 None:intergenic 50.0%
GGTGGATAAGCAAAAGGAGC+TGG - Chr8:24671781-24671800 MsG0880043429.01.T01:CDS 50.0%
TACCAGCAGGTGATCTCATG+TGG - Chr8:24671627-24671646 MsG0880043429.01.T01:CDS 50.0%
TCCACATGGTCTCTGATCCA+TGG + Chr8:24671419-24671438 None:intergenic 50.0%
TCCATGGATCAGAGACCATG+TGG - Chr8:24671415-24671434 MsG0880043429.01.T01:CDS 50.0%
TCTCCCCCGTTAAGATTCGA+GGG - Chr8:24671522-24671541 MsG0880043429.01.T01:CDS 50.0%
TCTTAACGGGGGAGAGATCA+AGG + Chr8:24671517-24671536 None:intergenic 50.0%
TGGCGAATTTGTGGGATCGA+AGG + Chr8:24671069-24671088 None:intergenic 50.0%
TGGTGGAAGATAAGGGAGGA+AGG + Chr8:24671242-24671261 None:intergenic 50.0%
TTCTTGATGGGAGACAGTGC+CGG - Chr8:24671452-24671471 MsG0880043429.01.T01:CDS 50.0%
TTTGCCCTCAGGCAAACACA+GGG + Chr8:24671654-24671673 None:intergenic 50.0%
TTTGTGGGATCGAAGGATGG+TGG + Chr8:24671062-24671081 None:intergenic 50.0%
! AATGATGGGGAGTTTGGCAG+CGG + Chr8:24671183-24671202 None:intergenic 50.0%
! ACGAATGGAGGTTTTCGCAG+CGG + Chr8:24671129-24671148 None:intergenic 50.0%
! GGTTTTCGCAGCGGTGTTTA+AGG + Chr8:24671120-24671139 None:intergenic 50.0%
! TCTTTAGAGAGAGCCGGTTG+CGG + Chr8:24671092-24671111 None:intergenic 50.0%
!! AGCAGGGATGGTGGAAGATA+AGG + Chr8:24671250-24671269 None:intergenic 50.0%
!! GCAGGGATGGTGGAAGATAA+GGG + Chr8:24671249-24671268 None:intergenic 50.0%
ACTGCATCATCATACGCGGC+TGG + Chr8:24671344-24671363 None:intergenic 55.0%
AGTTTGGCAGCGGAGGAAGA+CGG + Chr8:24671173-24671192 None:intergenic 55.0%
CACAAATTCGCCACCGCAAC+CGG - Chr8:24671076-24671095 MsG0880043429.01.T01:CDS 55.0%
CAGCTAATGTGGAGCAGGGA+TGG + Chr8:24671262-24671281 None:intergenic 55.0%
CTAATGTGGAGCAGGGATGG+TGG + Chr8:24671259-24671278 None:intergenic 55.0%
CTACGCTGGAGATCCATTGG+TGG - Chr8:24671763-24671782 MsG0880043429.01.T01:CDS 55.0%
CTCTCCCCCGTTAAGATTCG+AGG - Chr8:24671521-24671540 MsG0880043429.01.T01:CDS 55.0%
CTGCATCATCATACGCGGCT+GGG + Chr8:24671343-24671362 None:intergenic 55.0%
CTTTGCCCTCAGGCAAACAC+AGG + Chr8:24671655-24671674 None:intergenic 55.0%
GACCACATGAGATCACCTGC+TGG + Chr8:24671632-24671651 None:intergenic 55.0%
GGAAGATAAGGGAGGAAGGG+TGG + Chr8:24671238-24671257 None:intergenic 55.0%
GGTTGCGGTGGCGAATTTGT+GGG + Chr8:24671077-24671096 None:intergenic 55.0%
GTTCGGGAGGAAGAGACGAA+TGG + Chr8:24671144-24671163 None:intergenic 55.0%
GTTTGCCTGAGGGCAAAGAC+CGG - Chr8:24671657-24671676 MsG0880043429.01.T01:CDS 55.0%
TAAGGGAGGAAGGGTGGTAG+AGG + Chr8:24671232-24671251 None:intergenic 55.0%
TCAACGGAGGCAACAATGGC+AGG + Chr8:24671293-24671312 None:intergenic 55.0%
TGGCTACGCTGGAGATCCAT+TGG - Chr8:24671760-24671779 MsG0880043429.01.T01:CDS 55.0%
TGGGCAGCTAATGTGGAGCA+GGG + Chr8:24671266-24671285 None:intergenic 55.0%
TGGTCCCTGTGTTTGCCTGA+GGG - Chr8:24671647-24671666 MsG0880043429.01.T01:CDS 55.0%
TTGGGCAGCTAATGTGGAGC+AGG + Chr8:24671267-24671286 None:intergenic 55.0%
TTTGCCTGAGGGCAAAGACC+GGG - Chr8:24671658-24671677 MsG0880043429.01.T01:CDS 55.0%
!! GGGATGGTGGAAGATAAGGG+AGG + Chr8:24671246-24671265 None:intergenic 55.0%
AGGAAGACGGTGGAGGGTTC+GGG + Chr8:24671160-24671179 None:intergenic 60.0%
CATCATCATACGCGGCTGGG+AGG + Chr8:24671340-24671359 None:intergenic 60.0%
CGGGAGGAAGAGACGAATGG+AGG + Chr8:24671141-24671160 None:intergenic 60.0%
CGGTTGCGGTGGCGAATTTG+TGG + Chr8:24671078-24671097 None:intergenic 60.0%
GAGGAAGACGGTGGAGGGTT+CGG + Chr8:24671161-24671180 None:intergenic 60.0%
GTGGTCCCTGTGTTTGCCTG+AGG - Chr8:24671646-24671665 MsG0880043429.01.T01:CDS 60.0%
TTAGAGAGAGCCGGTTGCGG+TGG + Chr8:24671089-24671108 None:intergenic 60.0%
! ATCGAAGGATGGTGGCACGG+TGG + Chr8:24671054-24671073 None:intergenic 60.0%
! GGGATCGAAGGATGGTGGCA+CGG + Chr8:24671057-24671076 None:intergenic 60.0%
! TCGAAGGATGGTGGCACGGT+GGG + Chr8:24671053-24671072 None:intergenic 60.0%
! TTGATGGGAGACAGTGCCGG+AGG - Chr8:24671455-24671474 MsG0880043429.01.T01:CDS 60.0%
!! CGAAGATTTTGGCCGCACGC+GGG - Chr8:24671804-24671823 MsG0880043429.01.T01:CDS 60.0%
!! GAAGATTTTGGCCGCACGCG+GGG - Chr8:24671805-24671824 MsG0880043429.01.T01:CDS 60.0%
!! GCGAAGATTTTGGCCGCACG+CGG - Chr8:24671803-24671822 MsG0880043429.01.T01:CDS 60.0%
AAGACGGTGGAGGGTTCGGG+AGG + Chr8:24671157-24671176 None:intergenic 65.0%
CAGCGGAGGAAGACGGTGGA+GGG + Chr8:24671166-24671185 None:intergenic 65.0%
GATGGGGAGTTTGGCAGCGG+AGG + Chr8:24671180-24671199 None:intergenic 65.0%
TGGTCCCGGTCTTTGCCCTC+AGG + Chr8:24671665-24671684 None:intergenic 65.0%
TTGGCAGCGGAGGAAGACGG+TGG + Chr8:24671170-24671189 None:intergenic 65.0%
! CGAAGGATGGTGGCACGGTG+GGG + Chr8:24671052-24671071 None:intergenic 65.0%
GCAGCGGAGGAAGACGGTGG+AGG + Chr8:24671167-24671186 None:intergenic 70.0%
CTCCACGTGCACCCCGCGTG+CGG + Chr8:24671819-24671838 None:intergenic 75.0%
GCGGCTGGGAGGCGATGCTC+AGG + Chr8:24671329-24671348 None:intergenic 75.0%
! GGCCGCACGCGGGGTGCACG+TGG - Chr8:24671814-24671833 MsG0880043429.01.T01:CDS 85.0%
Chromosome Type Strat End Strand Name
Chr8 gene 24670981 24671967 24670981 ID=MsG0880043429.01;Name=MsG0880043429.01
Chr8 mRNA 24670981 24671967 24670981 ID=MsG0880043429.01.T01;Parent=MsG0880043429.01;Name=MsG0880043429.01.T01;_AED=0.48;_eAED=0.48;_QI=0|-1|0|1|-1|1|1|0|328
Chr8 exon 24670981 24671967 24670981 ID=MsG0880043429.01.T01:exon:9148;Parent=MsG0880043429.01.T01
Chr8 CDS 24670981 24671967 24670981 ID=MsG0880043429.01.T01:cds;Parent=MsG0880043429.01.T01
Gene Sequence

>MsG0880043429.01.T01

ATGGATCCCTATGAATTCCTCAAAATCAAACTAAACCCTGATGGTTCACTAACAAGAAACTACATTGTCCCCACCGTGCCACCATCCTTCGATCCCACAAATTCGCCACCGCAACCGGCTCTCTCTAAAGACATTCCCTTAAACACCGCTGCGAAAACCTCCATTCGTCTCTTCCTCCCGAACCCTCCACCGTCTTCCTCCGCTGCCAAACTCCCCATCATTCTCTACTTCCATGGTGGTGGTTTCATCCTCTACCACCCTTCCTCCCTTATCTTCCACCATCCCTGCTCCACATTAGCTGCCCAAATTCCTGCCATTGTTGCCTCCGTTGATTATCGTCTCTCGCCTGAGCATCGCCTCCCAGCCGCGTATGATGATGCAGTTGATTCTCTTCTCTGGCTCAAATCCCAAGCTCAAAATCCAACGGAATCTGATCCATGGATCAGAGACCATGTGGACTTTGATAAATGTTTCTTGATGGGAGACAGTGCCGGAGGTAATATAGCCTATTTCACGGGTCTACGTGCTCTCGACCTTGATCTCTCCCCCGTTAAGATTCGAGGGATCATAATGAAATATCCATTTTTTAGTGGAGTTCAGAGGACTGAATCCGAGCTTCGTTTGGTTAACGATCGTATATTGCCATTACCAGCAGGTGATCTCATGTGGTCCCTGTGTTTGCCTGAGGGCAAAGACCGGGACCACGAGTATTGCAATCCAACAACTTCTGATCAAGTATATGGCGAAAAGATCGGACGGTTGCCAAGGTGTTTTGTTAATGGCTACGCTGGAGATCCATTGGTGGATAAGCAAAAGGAGCTGGCGAAGATTTTGGCCGCACGCGGGGTGCACGTGGAGTCCTGTTTTGATGAAGATGGGTATCATGCTGTTGAAATCTTTGATCATTCTAAGGCACAAGTTTTGCTTGATAATGTTAAGAAATTTATACTCTCTGCTGTTTCTGTTGCTCCTCAATCCTCTATGTAA
Protein sequence

>MsG0880043429.01.T01

MDPYEFLKIKLNPDGSLTRNYIVPTVPPSFDPTNSPPQPALSKDIPLNTAAKTSIRLFLPNPPPSSSAAKLPIILYFHGGGFILYHPSSLIFHHPCSTLAAQIPAIVASVDYRLSPEHRLPAAYDDAVDSLLWLKSQAQNPTESDPWIRDHVDFDKCFLMGDSAGGNIAYFTGLRALDLDLSPVKIRGIIMKYPFFSGVQRTESELRLVNDRILPLPAGDLMWSLCLPEGKDRDHEYCNPTTSDQVYGEKIGRLPRCFVNGYAGDPLVDKQKELAKILAARGVHVESCFDEDGYHAVEIFDHSKAQVLLDNVKKFILSAVSVAPQSSM*