AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0880043548.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880043548.01.T01 MTR_8g038170 81.538 130 24 0 15 144 172 301 8.07e-69 210
MsG0880043548.01.T01 MTR_8g038170 81.538 130 24 0 15 144 172 301 8.07e-69 210
MsG0880043548.01.T01 MTR_5g063670 42.105 95 44 3 33 119 186 277 7.09e-15 70.5
MsG0880043548.01.T02 MTR_8g038170 97.297 148 4 0 1 148 1 148 1.77e-107 309
MsG0880043548.01.T02 MTR_8g038170 97.297 148 4 0 1 148 1 148 1.77e-107 309
MsG0880043548.01.T02 MTR_8g038220 40.690 145 74 2 5 148 13 146 5.25e-27 103
MsG0880043548.01.T02 MTR_8g038220 41.549 142 71 2 8 148 17 147 4.66e-26 100
MsG0880043548.01.T02 MTR_2g031980 40.000 145 75 2 5 148 14 147 4.83e-26 100
MsG0880043548.01.T02 MTR_5g063670 39.041 146 77 2 4 148 14 148 3.11e-24 95.9
MsG0880043548.01.T02 MTR_8g038210 38.356 146 77 3 4 148 13 146 2.51e-23 93.6
MsG0880043548.01.T02 MTR_3g018780 37.931 145 78 2 5 148 14 147 6.28e-23 92.4
MsG0880043548.01.T02 MTR_8g038150 38.028 142 73 3 4 141 14 144 1.11e-22 92.0
MsG0880043548.01.T02 MTR_3g018790 33.793 145 84 2 5 148 14 147 6.99e-22 89.7
MsG0880043548.01.T02 MTR_8g038210 37.241 145 78 3 5 148 14 146 1.75e-21 88.6
MsG0880043548.01.T02 MTR_0276s0050 35.211 142 80 2 8 148 18 148 3.37e-18 79.7
MsG0880043548.01.T02 MTR_8g038180 31.034 145 88 2 5 148 14 147 1.12e-17 78.2
MsG0880043548.01.T02 MTR_3g018790 41.096 73 43 0 76 148 21 93 2.60e-14 68.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880043548.01.T01 AT2G38750 42.963 135 73 3 15 146 158 291 2.24e-25 98.6
MsG0880043548.01.T01 AT2G38750 42.963 135 73 3 15 146 175 308 2.68e-25 98.6
MsG0880043548.01.T01 AT1G35720 37.600 125 62 5 6 121 163 280 1.44e-15 72.4
MsG0880043548.01.T01 AT5G10230 37.600 125 63 5 6 122 163 280 2.14e-12 63.5
MsG0880043548.01.T01 AT5G10220 39.796 98 48 4 33 122 188 282 4.44e-12 62.8
MsG0880043548.01.T02 AT2G38750 59.603 151 58 1 1 148 1 151 1.82e-60 189
MsG0880043548.01.T02 AT2G38750 60.902 133 52 0 16 148 2 134 2.58e-56 178
MsG0880043548.01.T02 AT5G12380 37.931 145 78 2 5 148 14 147 3.29e-24 95.9
MsG0880043548.01.T02 AT1G35720 37.838 148 80 2 2 148 12 148 2.12e-23 94.0
MsG0880043548.01.T02 AT5G65020 38.732 142 75 2 8 148 18 148 2.14e-22 91.3
MsG0880043548.01.T02 AT5G10230 37.500 144 80 2 5 148 15 148 8.24e-22 89.4
MsG0880043548.01.T02 AT5G65020 37.037 135 73 2 15 148 10 133 1.01e-19 83.6
MsG0880043548.01.T02 AT5G10220 36.552 145 80 3 5 148 15 148 2.49e-19 82.8
MsG0880043548.01.T02 AT2G38760 39.161 143 70 4 5 141 15 146 2.65e-19 82.8
MsG0880043548.01.T02 AT1G68090 30.986 142 86 3 8 148 18 148 8.95e-13 64.3
MsG0880043548.01.T02 AT1G68090 30.986 142 86 3 8 148 18 148 1.75e-12 63.9

Find 50 sgRNAs with CRISPR-Local

Find 189 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATGAAATTGAGCCTTTCTTT+AGG 0.253678 8:-26440238 None:intergenic
TGAAATTGAGCCTTTCTTTA+GGG 0.301910 8:-26440237 None:intergenic
GCTGAGTATAACAACTTATA+TGG 0.309287 8:+26441796 MsG0880043548.01.T01:CDS
AAGCACTATAAAGAGATTTC+TGG 0.312193 8:+26441326 MsG0880043548.01.T01:CDS
GCTAGGATCTTAATGACTTA+AGG 0.319355 8:+26441529 MsG0880043548.01.T01:intron
CTTTGTTCCTTCATATCTAT+AGG 0.341085 8:-26441161 None:intergenic
ATCAGAATGCAGTGGTGCTT+TGG 0.364820 8:+26440171 MsG0880043548.01.T01:intron
TTGAGGATGATGAAGTAATA+AGG 0.375348 8:+26441261 MsG0880043548.01.T01:CDS
AAGATCTTCAGAGGAACTAT+TGG 0.377291 8:+26440289 MsG0880043548.01.T01:five_prime_UTR
TGCTTTGGAGCATGCACCCT+TGG 0.391368 8:+26440186 MsG0880043548.01.T01:five_prime_UTR
TGATGAAGTAATAAGGATAT+TGG 0.398287 8:+26441268 MsG0880043548.01.T01:CDS
TTAATAAAAGAACAAACCTT+TGG 0.402834 8:+26441913 MsG0880043548.01.T01:three_prime_UTR
ACTTCATCATCCTCAACAAT+TGG 0.409615 8:-26441254 None:intergenic
TTGAGCATTCTTAATGGCAT+TGG 0.429639 8:-26441225 None:intergenic
GTTTCGTTTATGAAATAAAG+AGG 0.459516 8:+26442104 MsG0880043548.01.T01:three_prime_UTR
CAGAGGAACTATTGGGAGCT+AGG 0.463248 8:+26440297 MsG0880043548.01.T01:five_prime_UTR
AAGAAAGGCTCAATTTCATA+TGG 0.467362 8:+26440242 MsG0880043548.01.T01:five_prime_UTR
TTACCTTTCTATCATTGCCA+TGG 0.471161 8:-26440376 None:intergenic
AAAATTGAAGAGACTGCTAA+AGG 0.486337 8:+26441835 MsG0880043548.01.T01:CDS
AGATGCAACATCTTCTTCAA+TGG 0.491454 8:-26440348 None:intergenic
AGATCTTCAGAGGAACTATT+GGG 0.492249 8:+26440290 MsG0880043548.01.T01:five_prime_UTR
CATCCATGGCAATGATAGAA+AGG 0.496277 8:+26440373 MsG0880043548.01.T01:five_prime_UTR
TTAGCCTCCTCTAGCAATCA+AGG 0.503479 8:-26441881 None:intergenic
TAGAGCTGACATTGATATGA+AGG 0.504202 8:+26441766 MsG0880043548.01.T01:CDS
AGATGCACGTTTAGCAAAAG+AGG 0.507874 8:+26440214 MsG0880043548.01.T01:five_prime_UTR
TGGAATGGTCAAAGAGAGAA+TGG 0.508195 8:-26440328 None:intergenic
GCAAAAGAGGCCCTAAAGAA+AGG 0.511238 8:+26440227 MsG0880043548.01.T01:five_prime_UTR
GCTTTGGAGCATGCACCCTT+GGG 0.524661 8:+26440187 MsG0880043548.01.T01:five_prime_UTR
ATCTTTCAAAACATTTCCAA+AGG 0.525406 8:-26441929 None:intergenic
AAATTGAAGAGACTGCTAAA+GGG 0.525725 8:+26441836 MsG0880043548.01.T01:CDS
GTGAGTGCCTATAGATATGA+AGG 0.526990 8:+26441154 MsG0880043548.01.T01:exon
CAACATCTTCTTCAATGGAA+TGG 0.529527 8:-26440343 None:intergenic
GGAACTATTGGGAGCTAGGA+AGG 0.530013 8:+26440301 MsG0880043548.01.T01:five_prime_UTR
TTGCTAACCTTGATTGCTAG+AGG 0.531913 8:+26441874 MsG0880043548.01.T01:exon
ACATTGATATGAAGGAGATA+AGG 0.560737 8:+26441774 MsG0880043548.01.T01:CDS
AAACGTGCATCTCTTTCCCA+AGG 0.568596 8:-26440203 None:intergenic
AATATACTTGTGGAGTACAA+AGG 0.574763 8:-26441572 None:intergenic
GATGTTGCATCTCACATCCA+TGG 0.585008 8:+26440359 MsG0880043548.01.T01:five_prime_UTR
ACCTTGCTAAAATATACTTG+TGG 0.592183 8:-26441582 None:intergenic
TTCTGGCAAGAATCTTGAAG+AGG 0.605202 8:+26441343 MsG0880043548.01.T01:CDS
GACTGCTAAAGGGAACTACA+AGG 0.615438 8:+26441846 MsG0880043548.01.T01:CDS
TCAAAAGAAGCCAATTGTTG+AGG 0.621583 8:+26441244 MsG0880043548.01.T01:CDS
TAAAATAAATCAGAATGCAG+TGG 0.621993 8:+26440163 MsG0880043548.01.T01:intron
AGATGAAACTGCAAAATCAG+AGG 0.624947 8:+26441189 MsG0880043548.01.T01:CDS
CTAACCTTGATTGCTAGAGG+AGG 0.643976 8:+26441877 MsG0880043548.01.T01:exon
TTAACAATAATAACAACACA+AGG 0.646645 8:-26442036 None:intergenic
TGCATGCACAAGATCTTCAG+AGG 0.650638 8:+26440280 MsG0880043548.01.T01:five_prime_UTR
CTATAGATATGAAGGAACAA+AGG 0.657119 8:+26441162 MsG0880043548.01.T01:exon
AACGTGCATCTCTTTCCCAA+GGG 0.668764 8:-26440202 None:intergenic
TGCAGCATTGAAGATTGATG+TGG 0.671356 8:+26441703 MsG0880043548.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATTTCATTAAAATATATGA+TGG - Chr8:26439694-26439713 None:intergenic 10.0%
!! TATGTAGTAATAATTTAATA+TGG - Chr8:26438503-26438522 None:intergenic 10.0%
!!! AAAAATTCTAATTTGTTTTT+AGG + Chr8:26439846-26439865 MsG0880043548.01.T01:intron 10.0%
!!! AAAAATTCTAATTTTGTTTT+TGG - Chr8:26439751-26439770 None:intergenic 10.0%
!!! AATATGAATTTTTATGTTTT+TGG + Chr8:26439788-26439807 MsG0880043548.01.T01:intron 10.0%
!!! AATTTTCTTCTTAATTATAA+GGG - Chr8:26440063-26440082 None:intergenic 10.0%
!!! ATAATATATTCTTTTTGTTT+TGG - Chr8:26439044-26439063 None:intergenic 10.0%
!!! GATTATTTTTTTTAAGTTTA+AGG - Chr8:26439317-26439336 None:intergenic 10.0%
!!! TATATTTTCTTATTTGAAAA+TGG + Chr8:26438898-26438917 MsG0880043548.01.T01:intron 10.0%
!! AAAAGTTTAAAGAATATGTT+AGG + Chr8:26439987-26440006 MsG0880043548.01.T01:intron 15.0%
!! AAAGTTTAAAGAATATGTTA+GGG + Chr8:26439988-26440007 MsG0880043548.01.T01:intron 15.0%
!! AATCAAATGTTGATATATTT+AGG - Chr8:26439579-26439598 None:intergenic 15.0%
!! AATTTCATTAAAAACTATGA+AGG + Chr8:26439904-26439923 MsG0880043548.01.T01:intron 15.0%
!! ATCAAATATTGGTATATTTA+AGG + Chr8:26440019-26440038 MsG0880043548.01.T01:intron 15.0%
!! ATCAAATGTTGATATATTTA+GGG - Chr8:26439578-26439597 None:intergenic 15.0%
!! ATTAGAGTTTAATAAGTAAA+TGG + Chr8:26439549-26439568 MsG0880043548.01.T01:intron 15.0%
!! ATTGTTACATGAAAAAAAAA+TGG - Chr8:26440776-26440795 None:intergenic 15.0%
!! GTGAATTAGTATTATTTATA+TGG + Chr8:26441045-26441064 MsG0880043548.01.T01:intron 15.0%
!! TAATGAAATTTACTAGAAAT+GGG + Chr8:26439704-26439723 MsG0880043548.01.T01:intron 15.0%
!! TCAAATATTGGTATATTTAA+GGG + Chr8:26440020-26440039 MsG0880043548.01.T01:intron 15.0%
!! TTAATGAAATTTACTAGAAA+TGG + Chr8:26439703-26439722 MsG0880043548.01.T01:intron 15.0%
!! TTCATATATGAAAAATTTGA+AGG + Chr8:26439961-26439980 MsG0880043548.01.T01:intron 15.0%
!!! AAAAACCTTAGAAATATTTT+AGG - Chr8:26439610-26439629 None:intergenic 15.0%
!!! AATAAAAAGGATATGTTTTT+TGG + Chr8:26439075-26439094 MsG0880043548.01.T01:intron 15.0%
!!! AATTTTGATTAAACATATGA+AGG + Chr8:26438958-26438977 MsG0880043548.01.T01:intron 15.0%
!!! ATGACTTTTACAAAATAAAA+AGG + Chr8:26439062-26439081 MsG0880043548.01.T01:intron 15.0%
!!! GAATTTTCTTCTTAATTATA+AGG - Chr8:26440064-26440083 None:intergenic 15.0%
!!! TAAAATATTTCTAAGGTTTT+TGG + Chr8:26439609-26439628 MsG0880043548.01.T01:intron 15.0%
!!! TAACATATTCTTTAAACTTT+TGG - Chr8:26439988-26440007 None:intergenic 15.0%
!!! TATTTTCTATATACACTTAA+TGG - Chr8:26440949-26440968 None:intergenic 15.0%
!!! TGCATTTTTTTTTTGTATAT+AGG + Chr8:26441673-26441692 MsG0880043548.01.T01:intron 15.0%
!!! TGTTCAAAAAAATTTAAGAT+AGG - Chr8:26439120-26439139 None:intergenic 15.0%
!!! TTCTCAAAACATTTTTTTTT+TGG - Chr8:26440832-26440851 None:intergenic 15.0%
!!! TTTTAAGAATTCTTTTTGTT+TGG - Chr8:26439014-26439033 None:intergenic 15.0%
!!! TTTTTTTTCATGTAACAATA+TGG + Chr8:26440777-26440796 MsG0880043548.01.T01:intron 15.0%
!!! TTTTTTTTGTATATAGGTTT+TGG + Chr8:26441679-26441698 MsG0880043548.01.T01:intron 15.0%
!! AAGTTTAAAGAATATGTTAG+GGG + Chr8:26439989-26440008 MsG0880043548.01.T01:intron 20.0%
!! AATTTCATAAGTCATATGTT+TGG - Chr8:26441475-26441494 None:intergenic 20.0%
!! ATAAGGGTTAAATAAGTAAA+TGG - Chr8:26440047-26440066 None:intergenic 20.0%
!! ATATTTACATATCATTGTCT+AGG - Chr8:26440978-26440997 None:intergenic 20.0%
!! ATTAACCCAAAAATTAATGT+GGG - Chr8:26440650-26440669 None:intergenic 20.0%
!! ATTCATCAAATATCATCAAT+TGG - Chr8:26438586-26438605 None:intergenic 20.0%
!! TCAAATGTTGATATATTTAG+GGG - Chr8:26439577-26439596 None:intergenic 20.0%
!! TGTTATTATACAACAAAATG+AGG - Chr8:26441630-26441649 None:intergenic 20.0%
!! TTAACAATAATAACAACACA+AGG - Chr8:26442039-26442058 None:intergenic 20.0%
!! TTAATAAAAGAACAAACCTT+TGG + Chr8:26441913-26441932 MsG0880043548.01.T01:three_prime_UTR 20.0%
!! TTATGAATGTTAGATTTCAA+AGG + Chr8:26441016-26441035 MsG0880043548.01.T01:intron 20.0%
!! TTCATCAAATATCATCAATT+GGG - Chr8:26438585-26438604 None:intergenic 20.0%
!!! AAATCATTGATAACAGTATT+GGG + Chr8:26438641-26438660 MsG0880043548.01.T01:five_prime_UTR 20.0%
!!! AATTCCATGGTTTTAAATTA+TGG + Chr8:26440464-26440483 MsG0880043548.01.T01:intron 20.0%
!!! ACATTAATTTTTGGGTTAAT+CGG + Chr8:26440650-26440669 MsG0880043548.01.T01:intron 20.0%
!!! ACATTTTTTTTAGGGAATAA+TGG - Chr8:26439403-26439422 None:intergenic 20.0%
!!! AGCTAAGAACATTTTTTTTA+GGG - Chr8:26439411-26439430 None:intergenic 20.0%
!!! ATTTCATAGATGGAAAATTT+TGG - Chr8:26439639-26439658 None:intergenic 20.0%
!!! CTAGACTAAAAATTGATTTT+TGG + Chr8:26440674-26440693 MsG0880043548.01.T01:intron 20.0%
!!! GAAAATTATATTTTGTTGTG+TGG + Chr8:26440115-26440134 MsG0880043548.01.T01:intron 20.0%
!!! GAATTAATGATGTAATTTTC+AGG + Chr8:26438602-26438621 MsG0880043548.01.T01:intron 20.0%
!!! TAGCTAAGAACATTTTTTTT+AGG - Chr8:26439412-26439431 None:intergenic 20.0%
!!! TCTTTTAATAGAAATGTTGA+AGG + Chr8:26438875-26438894 MsG0880043548.01.T01:intron 20.0%
!!! TGTTTTTTGGTAGAAAAAAA+AGG + Chr8:26439088-26439107 MsG0880043548.01.T01:intron 20.0%
!!! TTTATTTATTACCTTTTGCT+AGG + Chr8:26441512-26441531 MsG0880043548.01.T01:intron 20.0%
!!! TTTCATAGATGGAAAATTTT+GGG - Chr8:26439638-26439657 None:intergenic 20.0%
!!! TTTTAATCTATCTAATTCCA+TGG + Chr8:26440451-26440470 MsG0880043548.01.T01:intron 20.0%
! AAAAGAAAAACATCATCATC+TGG - Chr8:26438861-26438880 None:intergenic 25.0%
! AAAATGTATTGCAATATGAC+AGG + Chr8:26439498-26439517 MsG0880043548.01.T01:intron 25.0%
! AGCAATTAAATATGATGAGT+AGG - Chr8:26438373-26438392 None:intergenic 25.0%
! AGTTTAAAGAATATGTTAGG+GGG + Chr8:26439990-26440009 MsG0880043548.01.T01:intron 25.0%
! ATCTTTCAAAACATTTCCAA+AGG - Chr8:26441932-26441951 None:intergenic 25.0%
! ATTATACAACAAAATGAGGT+TGG - Chr8:26441626-26441645 None:intergenic 25.0%
! CAAATGTTGATATATTTAGG+GGG - Chr8:26439576-26439595 None:intergenic 25.0%
! CAATTAAATATGATGAGTAG+GGG - Chr8:26438371-26438390 None:intergenic 25.0%
! CATTAAAAACTATGAAGGTT+AGG + Chr8:26439909-26439928 MsG0880043548.01.T01:intron 25.0%
! GATTAACCCAAAAATTAATG+TGG - Chr8:26440651-26440670 None:intergenic 25.0%
! GCAATTAAATATGATGAGTA+GGG - Chr8:26438372-26438391 None:intergenic 25.0%
! GTTTCGTTTATGAAATAAAG+AGG + Chr8:26442104-26442123 MsG0880043548.01.T01:three_prime_UTR 25.0%
! TAAAATAAATCAGAATGCAG+TGG + Chr8:26440163-26440182 MsG0880043548.01.T01:intron 25.0%
! TCAAAAACTTTCCAAGAATA+TGG + Chr8:26438413-26438432 MsG0880043548.01.T01:five_prime_UTR 25.0%
! TCTATATACACTTAATGGTA+GGG - Chr8:26440944-26440963 None:intergenic 25.0%
! TGATGAAGTAATAAGGATAT+TGG + Chr8:26441268-26441287 MsG0880043548.01.T01:CDS 25.0%
! TTCTATATACACTTAATGGT+AGG - Chr8:26440945-26440964 None:intergenic 25.0%
!! ACATGAGTTGACTAAAAATT+AGG - Chr8:26439667-26439686 None:intergenic 25.0%
!! ATAATTTTCCAACTTGTGAT+TGG - Chr8:26440104-26440123 None:intergenic 25.0%
!! CATGAGTTGACTAAAAATTA+GGG - Chr8:26439666-26439685 None:intergenic 25.0%
!! GAAATCATTGATAACAGTAT+TGG + Chr8:26438640-26438659 MsG0880043548.01.T01:five_prime_UTR 25.0%
!! TGTTAGATTTCAAAGGTTTT+AGG + Chr8:26441023-26441042 MsG0880043548.01.T01:intron 25.0%
!! TTCATAGATGGAAAATTTTG+GGG - Chr8:26439637-26439656 None:intergenic 25.0%
!!! AAAAATGTTTTGAGAAACGT+TGG + Chr8:26440836-26440855 MsG0880043548.01.T01:intron 25.0%
!!! AACCTTAGAAATATTTTAGG+AGG - Chr8:26439607-26439626 None:intergenic 25.0%
!!! TGTAAAATTTGCTTTTGCAT+AGG - Chr8:26439238-26439257 None:intergenic 25.0%
AAAATTGAAGAGACTGCTAA+AGG + Chr8:26441835-26441854 MsG0880043548.01.T01:CDS 30.0%
AAATTGAAGAGACTGCTAAA+GGG + Chr8:26441836-26441855 MsG0880043548.01.T01:CDS 30.0%
AAGAAAGGCTCAATTTCATA+TGG + Chr8:26440242-26440261 MsG0880043548.01.T01:five_prime_UTR 30.0%
AAGCACTATAAAGAGATTTC+TGG + Chr8:26441326-26441345 MsG0880043548.01.T01:CDS 30.0%
AATATACTTGTGGAGTACAA+AGG - Chr8:26441575-26441594 None:intergenic 30.0%
ACATTGATATGAAGGAGATA+AGG + Chr8:26441774-26441793 MsG0880043548.01.T01:CDS 30.0%
ACCTTGCTAAAATATACTTG+TGG - Chr8:26441585-26441604 None:intergenic 30.0%
AGAATAAACCAATCACAAGT+TGG + Chr8:26440093-26440112 MsG0880043548.01.T01:intron 30.0%
ATGAAAAAGTCAACACACAA+GGG - Chr8:26440142-26440161 None:intergenic 30.0%
ATGAAATTGAGCCTTTCTTT+AGG - Chr8:26440241-26440260 None:intergenic 30.0%
ATGCTAAAATAAAGGCCAAA+TGG + Chr8:26440886-26440905 MsG0880043548.01.T01:intron 30.0%
CTATAGATATGAAGGAACAA+AGG + Chr8:26441162-26441181 MsG0880043548.01.T01:exon 30.0%
CTCAATCCATGCTAAAATAA+AGG + Chr8:26440878-26440897 MsG0880043548.01.T01:intron 30.0%
CTTTGTTCCTTCATATCTAT+AGG - Chr8:26441164-26441183 None:intergenic 30.0%
GCTGAGTATAACAACTTATA+TGG + Chr8:26441796-26441815 MsG0880043548.01.T01:CDS 30.0%
TACATATCATTGTCTAGGTT+TGG - Chr8:26440973-26440992 None:intergenic 30.0%
TGAAATTGAGCCTTTCTTTA+GGG - Chr8:26440240-26440259 None:intergenic 30.0%
TGCTAAAATAAAGGCCAAAT+GGG + Chr8:26440887-26440906 MsG0880043548.01.T01:intron 30.0%
TTAGGGACTAATTTCATAGA+TGG - Chr8:26439649-26439668 None:intergenic 30.0%
TTGAGGATGATGAAGTAATA+AGG + Chr8:26441261-26441280 MsG0880043548.01.T01:CDS 30.0%
TTGATTGAATGCCATATTCT+TGG - Chr8:26438427-26438446 None:intergenic 30.0%
! ACGACCATAATTTAAAACCA+TGG - Chr8:26440471-26440490 None:intergenic 30.0%
! CATGAGTTGACTAAAAAGTA+GGG + Chr8:26439932-26439951 MsG0880043548.01.T01:intron 30.0%
! CTTCTTTTGAGCATTCTTAA+TGG - Chr8:26441234-26441253 None:intergenic 30.0%
! GATAACAGTATTGGGAAAAT+GGG + Chr8:26438649-26438668 MsG0880043548.01.T01:five_prime_UTR 30.0%
! GTCCTCCTAAAATATTTCTA+AGG + Chr8:26439602-26439621 MsG0880043548.01.T01:intron 30.0%
! TCATAGATGGAAAATTTTGG+GGG - Chr8:26439636-26439655 None:intergenic 30.0%
! TGATAACAGTATTGGGAAAA+TGG + Chr8:26438648-26438667 MsG0880043548.01.T01:five_prime_UTR 30.0%
!! ATGATGTAATTTTCAGGACA+TGG + Chr8:26438608-26438627 MsG0880043548.01.T01:intron 30.0%
!! CTTAAAACACTTGATTTGTC+CGG + Chr8:26440721-26440740 MsG0880043548.01.T01:intron 30.0%
!! TGATGTAATTTTCAGGACAT+GGG + Chr8:26438609-26438628 MsG0880043548.01.T01:intron 30.0%
!!! ATGGTTTTAAATTATGGTCG+TGG + Chr8:26440470-26440489 MsG0880043548.01.T01:intron 30.0%
!!! ATTTGGCCTTTATTTTAGCA+TGG - Chr8:26440887-26440906 None:intergenic 30.0%
!!! CAAGTGTTTTAAGAAATGTG+AGG - Chr8:26440714-26440733 None:intergenic 30.0%
!!! TCCACAAGTATATTTTAGCA+AGG + Chr8:26441581-26441600 MsG0880043548.01.T01:CDS 30.0%
!!! TGTTTTAAGAAATGTGAGGT+CGG - Chr8:26440710-26440729 None:intergenic 30.0%
AAGATCTTCAGAGGAACTAT+TGG + Chr8:26440289-26440308 MsG0880043548.01.T01:five_prime_UTR 35.0%
AATGGGATGTCATATTAGGT+TGG + Chr8:26440904-26440923 MsG0880043548.01.T01:intron 35.0%
ACCCAAAAATTAATGTGGGT+CGG - Chr8:26440646-26440665 None:intergenic 35.0%
ACCTAATATGACATCCCATT+TGG - Chr8:26440904-26440923 None:intergenic 35.0%
ACTTCATCATCCTCAACAAT+TGG - Chr8:26441257-26441276 None:intergenic 35.0%
AGATCTTCAGAGGAACTATT+GGG + Chr8:26440290-26440309 MsG0880043548.01.T01:five_prime_UTR 35.0%
AGATGAAACTGCAAAATCAG+AGG + Chr8:26441189-26441208 MsG0880043548.01.T01:CDS 35.0%
AGATGCAACATCTTCTTCAA+TGG - Chr8:26440351-26440370 None:intergenic 35.0%
ATCATGAACGCCAATTTCAA+AGG + Chr8:26438726-26438745 MsG0880043548.01.T01:five_prime_UTR 35.0%
CAACATCTTCTTCAATGGAA+TGG - Chr8:26440346-26440365 None:intergenic 35.0%
CACATCTAGATTGTGTAATC+CGG - Chr8:26440743-26440762 None:intergenic 35.0%
CATGAAAAAGTCAACACACA+AGG - Chr8:26440143-26440162 None:intergenic 35.0%
CTATAAGATTCTCTCTCCAA+GGG - Chr8:26438677-26438696 None:intergenic 35.0%
GCTAGGATCTTAATGACTTA+AGG + Chr8:26441529-26441548 MsG0880043548.01.T01:intron 35.0%
GGGGGACTAAAATCAAATAT+TGG + Chr8:26440008-26440027 MsG0880043548.01.T01:intron 35.0%
GTCATTAAGATCCTAGCAAA+AGG - Chr8:26441526-26441545 None:intergenic 35.0%
TAGAGCTGACATTGATATGA+AGG + Chr8:26441766-26441785 MsG0880043548.01.T01:CDS 35.0%
TCAAAAGAAGCCAATTGTTG+AGG + Chr8:26441244-26441263 MsG0880043548.01.T01:CDS 35.0%
TTACCTTTCTATCATTGCCA+TGG - Chr8:26440379-26440398 None:intergenic 35.0%
TTGAGCATTCTTAATGGCAT+TGG - Chr8:26441228-26441247 None:intergenic 35.0%
! ACATGAGTTTGTGCGTTTTA+AGG + Chr8:26438778-26438797 MsG0880043548.01.T01:five_prime_UTR 35.0%
! GAGCTGTTTCAAAAAGCAAT+AGG - Chr8:26441122-26441141 None:intergenic 35.0%
! GCATGAGTTGACTAAAAAGT+AGG + Chr8:26439931-26439950 MsG0880043548.01.T01:intron 35.0%
! TGAATTTAGCTCAGTTGGTA+CGG - Chr8:26441438-26441457 None:intergenic 35.0%
!! GATCATTCCACCTTTGAAAT+TGG - Chr8:26438739-26438758 None:intergenic 35.0%
!! GATGTAATTTTCAGGACATG+GGG + Chr8:26438610-26438629 MsG0880043548.01.T01:intron 35.0%
!!! ATGGCATTGGAAAGTGTTTT+TGG - Chr8:26441215-26441234 None:intergenic 35.0%
!!! CTGTAGTTTTTGATTTTGCG+CGG + Chr8:26440498-26440517 MsG0880043548.01.T01:intron 35.0%
!!! TTGCTTTTTGAAACAGCTCT+TGG + Chr8:26441123-26441142 MsG0880043548.01.T01:intron 35.0%
AGATGCACGTTTAGCAAAAG+AGG + Chr8:26440214-26440233 MsG0880043548.01.T01:five_prime_UTR 40.0%
ATGAACGCCAATTTCAAAGG+TGG + Chr8:26438729-26438748 MsG0880043548.01.T01:five_prime_UTR 40.0%
CATCCATGGCAATGATAGAA+AGG + Chr8:26440373-26440392 MsG0880043548.01.T01:five_prime_UTR 40.0%
CCCAAAAATTAATGTGGGTC+GGG - Chr8:26440645-26440664 None:intergenic 40.0%
CCTATAAGATTCTCTCTCCA+AGG - Chr8:26438678-26438697 None:intergenic 40.0%
CCTTGGAGAGAGAATCTTAT+AGG + Chr8:26438675-26438694 MsG0880043548.01.T01:five_prime_UTR 40.0%
GCCAAATGGGATGTCATATT+AGG + Chr8:26440900-26440919 MsG0880043548.01.T01:intron 40.0%
GCTTTAACTCAAGCTTTCTC+AGG + Chr8:26438456-26438475 MsG0880043548.01.T01:five_prime_UTR 40.0%
GTGAGTGCCTATAGATATGA+AGG + Chr8:26441154-26441173 MsG0880043548.01.T01:exon 40.0%
TGGAATGGTCAAAGAGAGAA+TGG - Chr8:26440331-26440350 None:intergenic 40.0%
TTCTGGCAAGAATCTTGAAG+AGG + Chr8:26441343-26441362 MsG0880043548.01.T01:CDS 40.0%
TTGCTAACCTTGATTGCTAG+AGG + Chr8:26441874-26441893 MsG0880043548.01.T01:exon 40.0%
! CCCGACCCACATTAATTTTT+GGG + Chr8:26440642-26440661 MsG0880043548.01.T01:intron 40.0%
! GCAGTCTCTTCAATTTTCTG+AGG - Chr8:26441832-26441851 None:intergenic 40.0%
! TGCAGCATTGAAGATTGATG+TGG + Chr8:26441703-26441722 MsG0880043548.01.T01:CDS 40.0%
AAACGTGCATCTCTTTCCCA+AGG - Chr8:26440206-26440225 None:intergenic 45.0%
AACGTGCATCTCTTTCCCAA+GGG - Chr8:26440205-26440224 None:intergenic 45.0%
ATCAGAATGCAGTGGTGCTT+TGG + Chr8:26440171-26440190 MsG0880043548.01.T01:intron 45.0%
ATTGGGAAAATGGGATCCCT+TGG + Chr8:26438658-26438677 MsG0880043548.01.T01:five_prime_UTR 45.0%
CCAACTGAGCTAAATTCACG+AGG + Chr8:26441440-26441459 MsG0880043548.01.T01:intron 45.0%
CTAACCTTGATTGCTAGAGG+AGG + Chr8:26441877-26441896 MsG0880043548.01.T01:exon 45.0%
GACTGCTAAAGGGAACTACA+AGG + Chr8:26441846-26441865 MsG0880043548.01.T01:CDS 45.0%
GATGTTGCATCTCACATCCA+TGG + Chr8:26440359-26440378 MsG0880043548.01.T01:five_prime_UTR 45.0%
GCAAAAGAGGCCCTAAAGAA+AGG + Chr8:26440227-26440246 MsG0880043548.01.T01:five_prime_UTR 45.0%
TGCATGCACAAGATCTTCAG+AGG + Chr8:26440280-26440299 MsG0880043548.01.T01:five_prime_UTR 45.0%
TTAGCCTCCTCTAGCAATCA+AGG - Chr8:26441884-26441903 None:intergenic 45.0%
! CCTCGTGAATTTAGCTCAGT+TGG - Chr8:26441443-26441462 None:intergenic 45.0%
! GCCCGACCCACATTAATTTT+TGG + Chr8:26440641-26440660 MsG0880043548.01.T01:intron 45.0%
!! GTAATTTTCAGGACATGGGG+TGG + Chr8:26438613-26438632 MsG0880043548.01.T01:intron 45.0%
CAGAGGAACTATTGGGAGCT+AGG + Chr8:26440297-26440316 MsG0880043548.01.T01:five_prime_UTR 50.0%
CTAGACCAGCTCGAAGCAAA+CGG + Chr8:26440614-26440633 MsG0880043548.01.T01:intron 50.0%
GGAACTATTGGGAGCTAGGA+AGG + Chr8:26440301-26440320 MsG0880043548.01.T01:five_prime_UTR 50.0%
CAGTTCCGTTTGCTTCGAGC+TGG - Chr8:26440622-26440641 None:intergenic 55.0%
!! GCTTTGGAGCATGCACCCTT+GGG + Chr8:26440187-26440206 MsG0880043548.01.T01:five_prime_UTR 55.0%
!! TGCTTTGGAGCATGCACCCT+TGG + Chr8:26440186-26440205 MsG0880043548.01.T01:five_prime_UTR 55.0%
Chromosome Type Strat End Strand Name
Chr8 gene 26438358 26442136 26438358 ID=MsG0880043548.01;Name=MsG0880043548.01
Chr8 mRNA 26438358 26442136 26438358 ID=MsG0880043548.01.T01;Parent=MsG0880043548.01;Name=MsG0880043548.01.T01;_AED=0.34;_eAED=0.34;_QI=546|1|1|1|0.8|0.83|6|256|149
Chr8 exon 26440176 26440394 26440176 ID=MsG0880043548.01.T01:exon:5249;Parent=MsG0880043548.01.T01
Chr8 exon 26441139 26441364 26441139 ID=MsG0880043548.01.T01:exon:5250;Parent=MsG0880043548.01.T01
Chr8 exon 26441534 26441602 26441534 ID=MsG0880043548.01.T01:exon:5251;Parent=MsG0880043548.01.T01
Chr8 exon 26441695 26442136 26441695 ID=MsG0880043548.01.T01:exon:5252;Parent=MsG0880043548.01.T01
Chr8 five_prime_UTR 26438358 26438477 26438358 ID=MsG0880043548.01.T01:five_prime_utr;Parent=MsG0880043548.01.T01
Chr8 five_prime_UTR 26438624 26438799 26438624 ID=MsG0880043548.01.T01:five_prime_utr;Parent=MsG0880043548.01.T01
Chr8 five_prime_UTR 26440176 26440394 26440176 ID=MsG0880043548.01.T01:five_prime_utr;Parent=MsG0880043548.01.T01
Chr8 five_prime_UTR 26441139 26441169 26441139 ID=MsG0880043548.01.T01:five_prime_utr;Parent=MsG0880043548.01.T01
Chr8 CDS 26441170 26441364 26441170 ID=MsG0880043548.01.T01:cds;Parent=MsG0880043548.01.T01
Chr8 CDS 26441534 26441602 26441534 ID=MsG0880043548.01.T01:cds;Parent=MsG0880043548.01.T01
Chr8 CDS 26441695 26441880 26441695 ID=MsG0880043548.01.T01:cds;Parent=MsG0880043548.01.T01
Chr8 three_prime_UTR 26441881 26442136 26441881 ID=MsG0880043548.01.T01:three_prime_utr;Parent=MsG0880043548.01.T01
Chr8 mRNA 26438358 26440424 26438358 ID=MsG0880043548.01.T02;Parent=MsG0880043548.01;Name=MsG0880043548.01.T02;_AED=0.43;_eAED=0.46;_QI=74|1|0.66|1|1|1|3|0|156
Chr8 exon 26440176 26440424 26440176 ID=MsG0880043548.01.T02:exon:5253;Parent=MsG0880043548.01.T02
Chr8 five_prime_UTR 26438358 26438431 26438358 ID=MsG0880043548.01.T02:five_prime_utr;Parent=MsG0880043548.01.T02
Chr8 CDS 26438432 26438477 26438432 ID=MsG0880043548.01.T02:cds;Parent=MsG0880043548.01.T02
Chr8 CDS 26438624 26438799 26438624 ID=MsG0880043548.01.T02:cds;Parent=MsG0880043548.01.T02
Chr8 CDS 26440176 26440424 26440176 ID=MsG0880043548.01.T02:cds;Parent=MsG0880043548.01.T02
Gene Sequence

>MsG0880043548.01.T01

ATGAAGGAACAAAGGTTAAAGATGAAACTGCAAAATCAGAGGCCAAAAACACTTTCCAATGCCATTAAGAATGCTCAAAAGAAGCCAATTGTTGAGGATGATGAAGTAATAAGGATATTGGCAACAAGAAGCAAGCTACATCTTCAAGCAGTTTACAAGCACTATAAAGAGATTTCTGGCAAGAATCTTGAAGAGGATCTTAATGACTTAAGGTTTAAAGAGACAGTGCAATGCCTTTGTACTCCACAAGTATATTTTAGCAAGGTTTTGGATGCAGCATTGAAGATTGATGTGGATAAGAATATTAAGAAATCACTTACTCGTGTGATTGTTACTAGAGCTGACATTGATATGAAGGAGATAAGGCTGAGTATAACAACTTATATGGAGTTTCTTTACCTCAGAAAATTGAAGAGACTGCTAAAGGGAACTACAAGGATTTCTTGCTAA

>MsG0880043548.01.T02

ATGGCATTCAATCAAGAGTTAGAAGCTTTAACTCAAGCTTTCTCAGGACATGGGGTGGATGAGAAATCATTGATAACAGTATTGGGAAAATGGGATCCCTTGGAGAGAGAATCTTATAGGAAGAAGACATCACATTTCTTCATTGAAGATCATGAACGCCAATTTCAAAGGTGGAATGATCATTGTGTTCGTCTTCTCAAACATGAGTTTGTGCGTTTTAAGAATGCAGTGGTGCTTTGGAGCATGCACCCTTGGGAAAGAGATGCACGTTTAGCAAAAGAGGCCCTAAAGAAAGGCTCAATTTCATATGGTGTATTGATTGAGATTGCATGCACAAGATCTTCAGAGGAACTATTGGGAGCTAGGAAGGCATACCATTCTCTCTTTGACCATTCCATTGAAGAAGATGTTGCATCTCACATCCATGGCAATGATAGAAAGGTAAACAAGACATCAAGTTGTACTATATAG
Protein sequence

>MsG0880043548.01.T01

MKEQRLKMKLQNQRPKTLSNAIKNAQKKPIVEDDEVIRILATRSKLHLQAVYKHYKEISGKNLEEDLNDLRFKETVQCLCTPQVYFSKVLDAALKIDVDKNIKKSLTRVIVTRADIDMKEIRLSITTYMEFLYLRKLKRLLKGTTRISC*

>MsG0880043548.01.T02

MAFNQELEALTQAFSGHGVDEKSLITVLGKWDPLERESYRKKTSHFFIEDHERQFQRWNDHCVRLLKHEFVRFKNAVVLWSMHPWERDARLAKEALKKGSISYGVLIEIACTRSSEELLGARKAYHSLFDHSIEEDVASHIHGNDRKVNKTSSCTI*