Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880043976.01.T01 | XP_013445246.1 | 91.667 | 96 | 8 | 0 | 23 | 118 | 61 | 156 | 4.18E-56 | 181 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880043976.01.T01 | Q9SSK7 | 37.234 | 94 | 57 | 2 | 23 | 114 | 223 | 316 | 6.82E-13 | 66.6 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880043976.01.T01 | I3SJ62 | 91.667 | 96 | 8 | 0 | 23 | 118 | 61 | 156 | 2.00e-56 | 181 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0880043971.01 | MsG0880043976.01 | 0.862855 | 3.770265e-64 | 1.120416e-60 |
| MsG0880043975.01 | MsG0880043976.01 | 0.867139 | 1.704857e-65 | 5.888465e-62 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880043976.01.T01 | MTR_8g045695 | 91.667 | 96 | 8 | 0 | 23 | 118 | 61 | 156 | 5.06e-60 | 181 |
| MsG0880043976.01.T01 | MTR_8g045735 | 91.489 | 94 | 8 | 0 | 23 | 116 | 61 | 154 | 2.59e-59 | 179 |
| MsG0880043976.01.T01 | MTR_8g045665 | 84.375 | 96 | 15 | 0 | 23 | 118 | 61 | 156 | 2.10e-55 | 170 |
| MsG0880043976.01.T01 | MTR_8g045560 | 82.474 | 97 | 17 | 0 | 22 | 118 | 60 | 156 | 1.39e-53 | 165 |
| MsG0880043976.01.T01 | MTR_8g045640 | 82.474 | 97 | 17 | 0 | 22 | 118 | 60 | 156 | 1.89e-53 | 165 |
| MsG0880043976.01.T01 | MTR_8g045570 | 73.913 | 92 | 23 | 1 | 23 | 113 | 61 | 152 | 1.30e-44 | 142 |
| MsG0880043976.01.T01 | MTR_8g045520 | 70.103 | 97 | 28 | 1 | 23 | 118 | 61 | 157 | 4.99e-44 | 141 |
| MsG0880043976.01.T01 | MTR_8g045555 | 68.478 | 92 | 28 | 1 | 23 | 113 | 61 | 152 | 3.75e-42 | 136 |
| MsG0880043976.01.T01 | MTR_8g045490 | 67.010 | 97 | 30 | 2 | 23 | 118 | 61 | 156 | 5.15e-41 | 133 |
| MsG0880043976.01.T01 | MTR_8g045300 | 64.583 | 96 | 33 | 1 | 23 | 117 | 61 | 156 | 1.11e-40 | 132 |
| MsG0880043976.01.T01 | MTR_8g045400 | 64.948 | 97 | 32 | 2 | 23 | 118 | 61 | 156 | 1.26e-40 | 132 |
| MsG0880043976.01.T01 | MTR_1g030810 | 40.659 | 91 | 51 | 2 | 25 | 112 | 62 | 152 | 1.95e-18 | 75.9 |
| MsG0880043976.01.T01 | MTR_8g012550 | 38.462 | 91 | 54 | 2 | 26 | 115 | 66 | 155 | 2.15e-17 | 73.2 |
| MsG0880043976.01.T01 | MTR_1g030820 | 38.462 | 91 | 53 | 2 | 25 | 112 | 62 | 152 | 5.06e-17 | 72.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880043976.01.T01 | AT1G70870 | 42.105 | 95 | 52 | 3 | 20 | 112 | 45 | 138 | 7.86e-17 | 71.6 |
| MsG0880043976.01.T01 | AT5G28010 | 35.106 | 94 | 59 | 2 | 23 | 114 | 73 | 166 | 1.85e-14 | 65.9 |
| MsG0880043976.01.T01 | AT5G28010 | 35.106 | 94 | 59 | 2 | 23 | 114 | 73 | 166 | 1.85e-14 | 65.9 |
| MsG0880043976.01.T01 | AT1G70850 | 37.234 | 94 | 57 | 2 | 23 | 114 | 223 | 316 | 6.96e-14 | 66.6 |
| MsG0880043976.01.T01 | AT1G70850 | 37.234 | 94 | 57 | 2 | 23 | 114 | 223 | 316 | 6.96e-14 | 66.6 |
| MsG0880043976.01.T01 | AT5G28000 | 35.714 | 98 | 60 | 3 | 20 | 114 | 67 | 164 | 4.80e-13 | 62.4 |
| MsG0880043976.01.T01 | AT1G70840 | 34.043 | 94 | 60 | 2 | 23 | 114 | 78 | 171 | 4.84e-13 | 62.4 |
| MsG0880043976.01.T01 | AT1G70830 | 35.106 | 94 | 59 | 2 | 23 | 114 | 80 | 173 | 7.56e-13 | 62.0 |
| MsG0880043976.01.T01 | AT1G70830 | 34.737 | 95 | 60 | 2 | 23 | 115 | 80 | 174 | 7.94e-13 | 62.4 |
| MsG0880043976.01.T01 | AT1G24020 | 38.272 | 81 | 48 | 2 | 33 | 112 | 72 | 151 | 1.75e-12 | 60.5 |
| MsG0880043976.01.T01 | AT1G24020 | 38.272 | 81 | 48 | 2 | 33 | 112 | 72 | 151 | 1.75e-12 | 60.5 |
| MsG0880043976.01.T01 | AT1G70830 | 34.737 | 95 | 60 | 2 | 23 | 115 | 80 | 174 | 3.10e-12 | 61.2 |
| MsG0880043976.01.T01 | AT1G70890 | 34.043 | 94 | 60 | 2 | 23 | 114 | 65 | 158 | 1.37e-11 | 58.2 |
| MsG0880043976.01.T01 | AT1G23120 | 32.979 | 94 | 60 | 3 | 19 | 111 | 54 | 145 | 2.70e-11 | 57.4 |
| MsG0880043976.01.T01 | AT1G70880 | 29.787 | 94 | 64 | 2 | 23 | 114 | 66 | 159 | 3.35e-11 | 57.4 |
| MsG0880043976.01.T01 | AT1G23120 | 33.333 | 93 | 59 | 3 | 20 | 111 | 105 | 195 | 4.54e-11 | 57.8 |
Find 30 sgRNAs with CRISPR-Local
Find 76 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAGATAACTTTCAAGTTGTT+TGG | 0.327904 | 8:-33669333 | MsG0880043976.01.T01:CDS |
| ATGGAAGTTCTGCTGTTAGA+TGG | 0.407851 | 8:-33669251 | MsG0880043976.01.T01:CDS |
| AATTCAAGTTCGAGTTCAAC+TGG | 0.415188 | 8:-33670337 | MsG0880043976.01.T01:CDS |
| TGGAGATATTGAAAACTATA+AGG | 0.439418 | 8:-33669310 | MsG0880043976.01.T01:CDS |
| CATGAGTTGCTTGGTATAGC+AGG | 0.455659 | 8:+33670365 | None:intergenic |
| AGGTAGTCCATCCAGCCATT+TGG | 0.456227 | 8:+33669189 | None:intergenic |
| AATTCCCCACATGAGTTGCT+TGG | 0.459855 | 8:+33670356 | None:intergenic |
| AAAAGCACTAGAGATATTGA+TGG | 0.477335 | 8:-33669162 | MsG0880043976.01.T01:CDS |
| ATAACTTTCAAGTTGTTTGG+TGG | 0.479625 | 8:-33669330 | MsG0880043976.01.T01:CDS |
| CGAGTTCAACTGGATGGTTG+AGG | 0.483822 | 8:-33670327 | MsG0880043976.01.T01:CDS |
| GAGTTCAACTGGATGGTTGA+GGG | 0.500056 | 8:-33670326 | MsG0880043976.01.T01:intron |
| TCATCTTGTCAAAGGGGAAA+AGG | 0.504175 | 8:-33669139 | MsG0880043976.01.T01:CDS |
| TTGATGGTCATCTTGTCAAA+GGG | 0.506740 | 8:-33669146 | MsG0880043976.01.T01:CDS |
| GAGGATATTGATACTCCAAA+TGG | 0.508694 | 8:-33669204 | MsG0880043976.01.T01:CDS |
| CAAGTTCGAGTTCAACTGGA+TGG | 0.509876 | 8:-33670333 | MsG0880043976.01.T01:CDS |
| TAACATAACAACAGATGGTA+AGG | 0.513916 | 8:-33669403 | MsG0880043976.01.T01:intron |
| ATTGATGGTCATCTTGTCAA+AGG | 0.543582 | 8:-33669147 | MsG0880043976.01.T01:CDS |
| ATATTGATACTCCAAATGGC+TGG | 0.546067 | 8:-33669200 | MsG0880043976.01.T01:CDS |
| TGATACTCCAAATGGCTGGA+TGG | 0.568648 | 8:-33669196 | MsG0880043976.01.T01:CDS |
| GAAGTGCTTAACAAGGCTGA+TGG | 0.580204 | 8:-33669270 | MsG0880043976.01.T01:CDS |
| CTATACCAAGCAACTCATGT+GGG | 0.591208 | 8:-33670361 | MsG0880043976.01.T01:CDS |
| AGGCACACACATGCAATGAG+AGG | 0.626711 | 8:-33669383 | MsG0880043976.01.T01:CDS |
| TTGCTTGGTATAGCAGGTGT+GGG | 0.632870 | 8:+33670371 | None:intergenic |
| GCTATACCAAGCAACTCATG+TGG | 0.632973 | 8:-33670362 | MsG0880043976.01.T01:CDS |
| CATCTTAGAAGTGCTTAACA+AGG | 0.644958 | 8:-33669277 | MsG0880043976.01.T01:CDS |
| TGATGGTCATCTTGTCAAAG+GGG | 0.652198 | 8:-33669145 | MsG0880043976.01.T01:CDS |
| GGCACACACATGCAATGAGA+GGG | 0.653417 | 8:-33669382 | MsG0880043976.01.T01:CDS |
| GTTGCTTGGTATAGCAGGTG+TGG | 0.657481 | 8:+33670370 | None:intergenic |
| TATACCAAGCAACTCATGTG+GGG | 0.680077 | 8:-33670360 | MsG0880043976.01.T01:CDS |
| TGATTATGAGAAGAACAATG+AGG | 0.681814 | 8:-33669223 | MsG0880043976.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AAAATGTTTTTTATTGTTTT+TGG | - | Chr8:33670066-33670085 | MsG0880043976.01.T01:intron | 10.0% |
| !!! | TAAATTTTCACTTTTTGTTT+TGG | + | Chr8:33669595-33669614 | None:intergenic | 15.0% |
| !!! | TAAGATAAAGTTTTTACTTT+TGG | - | Chr8:33669981-33670000 | MsG0880043976.01.T01:intron | 15.0% |
| !!! | TATTAAAGTCAAACTTTTAT+CGG | - | Chr8:33669720-33669739 | MsG0880043976.01.T01:intron | 15.0% |
| !! | ATCAAAAATCAAATATCACA+AGG | + | Chr8:33669691-33669710 | None:intergenic | 20.0% |
| !! | ATCATAAAAACCTAATGATA+AGG | + | Chr8:33669817-33669836 | None:intergenic | 20.0% |
| !!! | ATTATTGTATTTTCTCTCTT+TGG | + | Chr8:33669484-33669503 | None:intergenic | 20.0% |
| !!! | TGGTGAAACTAATATTTTTA+AGG | - | Chr8:33669842-33669861 | MsG0880043976.01.T01:intron | 20.0% |
| !!! | TTAGGTTTTTATGATTTCTT+TGG | - | Chr8:33669822-33669841 | MsG0880043976.01.T01:intron | 20.0% |
| !!! | TTATTGTATTTTCTCTCTTT+GGG | + | Chr8:33669483-33669502 | None:intergenic | 20.0% |
| ! | AATGCTAACAAATGTTCTTA+AGG | + | Chr8:33670021-33670040 | None:intergenic | 25.0% |
| ! | ACAATATCTCTTGGAATTTA+CGG | + | Chr8:33669643-33669662 | None:intergenic | 25.0% |
| ! | ATAAAATTGCAGAATGATAG+AGG | - | Chr8:33669347-33669366 | MsG0880043976.01.T01:CDS | 25.0% |
| ! | ATAGAGGGAAATTATTGTTA+AGG | - | Chr8:33669363-33669382 | MsG0880043976.01.T01:CDS | 25.0% |
| ! | CAAATCAAATTGTGTTCAAA+TGG | + | Chr8:33669536-33669555 | None:intergenic | 25.0% |
| ! | TAAAATTGCAGAATGATAGA+GGG | - | Chr8:33669348-33669367 | MsG0880043976.01.T01:CDS | 25.0% |
| ! | TGGAGATATTGAAAACTATA+AGG | - | Chr8:33670195-33670214 | MsG0880043976.01.T01:intron | 25.0% |
| !!! | AAGATAACTTTCAAGTTGTT+TGG | - | Chr8:33670172-33670191 | MsG0880043976.01.T01:intron | 25.0% |
| !!! | AATTTTTCGATCTAGTGTAA+TGG | - | Chr8:33669217-33669236 | MsG0880043976.01.T01:CDS | 25.0% |
| !!! | ATTTTACTAATGAAGAGTAC+AGG | - | Chr8:33669444-33669463 | MsG0880043976.01.T01:intron | 25.0% |
| !!! | ATTTTCACTTTTTGTTTTGG+TGG | + | Chr8:33669592-33669611 | None:intergenic | 25.0% |
| !!! | CTGTTTAGGTTATTTTGATT+AGG | + | Chr8:33669566-33669585 | None:intergenic | 25.0% |
| !!! | GTAATGGATAAGTAATTACA+TGG | - | Chr8:33669233-33669252 | MsG0880043976.01.T01:CDS | 25.0% |
| !!! | TATTTGATTTTTGATCCTTG+TGG | - | Chr8:33669696-33669715 | MsG0880043976.01.T01:intron | 25.0% |
| CTTAGGGTTAATAAAACGTA+TGG | - | Chr8:33669401-33669420 | MsG0880043976.01.T01:intron | 30.0% | |
| GCTACAAAGTTAATCTCTTA+GGG | - | Chr8:33669385-33669404 | MsG0880043976.01.T01:CDS | 30.0% | |
| GTTTGATACGATAGCAAAAA+GGG | + | Chr8:33669621-33669640 | None:intergenic | 30.0% | |
| TAACATAACAACAGATGGTA+AGG | - | Chr8:33670102-33670121 | MsG0880043976.01.T01:intron | 30.0% | |
| TAATTACATGGCACATATCT+AGG | - | Chr8:33669245-33669264 | MsG0880043976.01.T01:CDS | 30.0% | |
| TGATTATGAGAAGAACAATG+AGG | - | Chr8:33670282-33670301 | MsG0880043976.01.T01:intron | 30.0% | |
| ! | GTTTGACTTTAATAACCACA+AGG | + | Chr8:33669714-33669733 | None:intergenic | 30.0% |
| ! | TGATTTTAAACTATCCTGTG+AGG | - | Chr8:33669297-33669316 | MsG0880043976.01.T01:CDS | 30.0% |
| !! | AAAAGCACTAGAGATATTGA+TGG | - | Chr8:33670343-33670362 | MsG0880043976.01.T01:CDS | 30.0% |
| !! | ATAACTTTCAAGTTGTTTGG+TGG | - | Chr8:33670175-33670194 | MsG0880043976.01.T01:intron | 30.0% |
| AATCCTTAACTAGTGCTCTA+AGG | + | Chr8:33669884-33669903 | None:intergenic | 35.0% | |
| ACCATCTGTTGTTATGTTAG+AGG | + | Chr8:33670101-33670120 | None:intergenic | 35.0% | |
| ATATTGATACTCCAAATGGC+TGG | - | Chr8:33670305-33670324 | MsG0880043976.01.T01:intron | 35.0% | |
| ATCCTTAACTAGTGCTCTAA+GGG | + | Chr8:33669883-33669902 | None:intergenic | 35.0% | |
| CCAAGAGATATTGTGCTTTA+TGG | - | Chr8:33669649-33669668 | MsG0880043976.01.T01:intron | 35.0% | |
| CCATAAAGCACAATATCTCT+TGG | + | Chr8:33669652-33669671 | None:intergenic | 35.0% | |
| GAGGATATTGATACTCCAAA+TGG | - | Chr8:33670301-33670320 | MsG0880043976.01.T01:intron | 35.0% | |
| GATTAACTTTGTAACCTCAC+AGG | + | Chr8:33669314-33669333 | None:intergenic | 35.0% | |
| GGCTACAAAGTTAATCTCTT+AGG | - | Chr8:33669384-33669403 | MsG0880043976.01.T01:CDS | 35.0% | |
| GGTTTGATACGATAGCAAAA+AGG | + | Chr8:33669622-33669641 | None:intergenic | 35.0% | |
| TGGCACATATCTAGGTAAAA+CGG | - | Chr8:33669253-33669272 | MsG0880043976.01.T01:CDS | 35.0% | |
| TGTGTTCAAATGGAACTTTG+TGG | + | Chr8:33669526-33669545 | None:intergenic | 35.0% | |
| ! | AATTCAAGTTCGAGTTCAAC+TGG | - | Chr8:33669168-33669187 | MsG0880043976.01.T01:CDS | 35.0% |
| ! | ATTGATGGTCATCTTGTCAA+AGG | - | Chr8:33670358-33670377 | MsG0880043976.01.T01:CDS | 35.0% |
| ! | GCATCGATATCCTTATCATT+AGG | - | Chr8:33669804-33669823 | MsG0880043976.01.T01:intron | 35.0% |
| ! | TATCTCTAGTGCTTTTGCTA+AGG | + | Chr8:33670339-33670358 | None:intergenic | 35.0% |
| ! | TTGATGGTCATCTTGTCAAA+GGG | - | Chr8:33670359-33670378 | MsG0880043976.01.T01:CDS | 35.0% |
| !! | CATCTTAGAAGTGCTTAACA+AGG | - | Chr8:33670228-33670247 | MsG0880043976.01.T01:intron | 35.0% |
| ATGGAACTTTGTGGTCGTAA+TGG | + | Chr8:33669517-33669536 | None:intergenic | 40.0% | |
| ATGGAAGTTCTGCTGTTAGA+TGG | - | Chr8:33670254-33670273 | MsG0880043976.01.T01:intron | 40.0% | |
| CTATACCAAGCAACTCATGT+GGG | - | Chr8:33669144-33669163 | MsG0880043976.01.T01:CDS | 40.0% | |
| GCCTCTAACATAACAACAGA+TGG | - | Chr8:33670097-33670116 | MsG0880043976.01.T01:intron | 40.0% | |
| TATACCAAGCAACTCATGTG+GGG | - | Chr8:33669145-33669164 | MsG0880043976.01.T01:CDS | 40.0% | |
| TCATCTTGTCAAAGGGGAAA+AGG | - | Chr8:33670366-33670385 | MsG0880043976.01.T01:CDS | 40.0% | |
| TGATGGTCATCTTGTCAAAG+GGG | - | Chr8:33670360-33670379 | MsG0880043976.01.T01:CDS | 40.0% | |
| ! | TTCCCTTAGAGCACTAGTTA+AGG | - | Chr8:33669878-33669897 | MsG0880043976.01.T01:intron | 40.0% |
| !!! | TGTTTTGGTGGCAACTGTTT+AGG | + | Chr8:33669580-33669599 | None:intergenic | 40.0% |
| AATTCCCCACATGAGTTGCT+TGG | + | Chr8:33669152-33669171 | None:intergenic | 45.0% | |
| CATGAGTTGCTTGGTATAGC+AGG | + | Chr8:33669143-33669162 | None:intergenic | 45.0% | |
| GAGTTCAACTGGATGGTTGA+GGG | - | Chr8:33669179-33669198 | MsG0880043976.01.T01:CDS | 45.0% | |
| GCTATACCAAGCAACTCATG+TGG | - | Chr8:33669143-33669162 | MsG0880043976.01.T01:CDS | 45.0% | |
| TGATACTCCAAATGGCTGGA+TGG | - | Chr8:33670309-33670328 | MsG0880043976.01.T01:intron | 45.0% | |
| TTGCTTGGTATAGCAGGTGT+GGG | + | Chr8:33669137-33669156 | None:intergenic | 45.0% | |
| ! | CAAGTTCGAGTTCAACTGGA+TGG | - | Chr8:33669172-33669191 | MsG0880043976.01.T01:CDS | 45.0% |
| ! | GAAGTGCTTAACAAGGCTGA+TGG | - | Chr8:33670235-33670254 | MsG0880043976.01.T01:intron | 45.0% |
| !! | GTTGAGGGTTAGTCTTGACT+TGG | - | Chr8:33669194-33669213 | MsG0880043976.01.T01:CDS | 45.0% |
| !! | TTAAAGTAATATTAAAAATA+AGG | + | Chr8:33670045-33670064 | None:intergenic | 5.0% |
| AGGCACACACATGCAATGAG+AGG | - | Chr8:33670122-33670141 | MsG0880043976.01.T01:intron | 50.0% | |
| AGGTAGTCCATCCAGCCATT+TGG | + | Chr8:33670319-33670338 | None:intergenic | 50.0% | |
| CGAGTTCAACTGGATGGTTG+AGG | - | Chr8:33669178-33669197 | MsG0880043976.01.T01:CDS | 50.0% | |
| GGCACACACATGCAATGAGA+GGG | - | Chr8:33670123-33670142 | MsG0880043976.01.T01:intron | 50.0% | |
| GTTGCTTGGTATAGCAGGTG+TGG | + | Chr8:33669138-33669157 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr8 | gene | 33669128 | 33670399 | 33669128 | ID=MsG0880043976.01;Name=MsG0880043976.01 |
| Chr8 | mRNA | 33669128 | 33670399 | 33669128 | ID=MsG0880043976.01.T01;Parent=MsG0880043976.01;Name=MsG0880043976.01.T01;_AED=0.50;_eAED=0.61;_QI=0|0|0|1|1|1|2|0|118 |
| Chr8 | exon | 33670327 | 33670399 | 33670327 | ID=MsG0880043976.01.T01:exon:10235;Parent=MsG0880043976.01.T01 |
| Chr8 | exon | 33669128 | 33669411 | 33669128 | ID=MsG0880043976.01.T01:exon:10234;Parent=MsG0880043976.01.T01 |
| Chr8 | CDS | 33670327 | 33670399 | 33670327 | ID=MsG0880043976.01.T01:cds;Parent=MsG0880043976.01.T01 |
| Chr8 | CDS | 33669128 | 33669411 | 33669128 | ID=MsG0880043976.01.T01:cds;Parent=MsG0880043976.01.T01 |
| Gene Sequence |
| Protein sequence |
