AlfalfaGEDB | MsG0880043971.01

M. sativa cultivar ZhongmuNo.1 / MsG0880043971.01

KEGG: -
GO: GO:0006952 GO:0009607
PFAM: PF00407

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880043971.01.T02	XP_013445246.1	93.617	94	6	0	1	94	63	156	3.98E-57	183
MsG0880043971.01.T01	XP_013445246.1	93.617	94	6	0	5	98	63	156	3.42E-57	183

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880043971.01.T01	P85524	41.758	91	51	2	3	92	61	150	1.41E-14	67.8
MsG0880043971.01.T02	P85524	41.573	89	50	2	1	88	63	150	3.86E-14	66.6

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880043971.01.T01	I3SJ62	93.617	94	6	0	5	98	63	156	1.63e-57	183
MsG0880043971.01.T02	I3SJ62	93.617	94	6	0	1	94	63	156	1.90e-57	183

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0880043971.01	MsG0880043975.01	0.921585	2.961824e-88	1.068469e-83
MsG0880043971.01	MsG0880043976.01	0.862855	3.770265e-64	1.120416e-60
MsG0880043971.01	MsG0880043985.01	0.819675	1.025198e-52	7.941505e-50
MsG0880043971.01	MsG0880043992.01	0.840595	7.908559e-58	1.131962e-54
MsG0880043971.01	MsG0880043994.01	0.810618	1.053851e-50	6.383154e-48
MsG0880043971.01	MsG0880044131.01	0.807764	4.310030e-50	2.421031e-47
MsG0480018985.01	MsG0880043971.01	0.809916	1.493299e-50	8.879356e-48
MsG0780039443.01	MsG0880043971.01	0.814094	1.835092e-51	1.219882e-48

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880043971.01.T02	MTR_8g045695	93.617	94	6	0	1	94	63	156	4.82e-61	183
MsG0880043971.01.T02	MTR_8g045735	94.565	92	5	0	1	92	63	154	1.47e-60	182
MsG0880043971.01.T02	MTR_8g045665	87.234	94	12	0	1	94	63	156	7.59e-57	172
MsG0880043971.01.T02	MTR_8g045560	86.170	94	13	0	1	94	63	156	5.88e-55	167
MsG0880043971.01.T02	MTR_8g045640	86.170	94	13	0	1	94	63	156	1.06e-54	167
MsG0880043971.01.T02	MTR_8g045520	72.632	95	25	1	1	94	63	157	1.09e-45	144
MsG0880043971.01.T02	MTR_8g045570	74.444	90	22	1	1	89	63	152	4.64e-45	142
MsG0880043971.01.T02	MTR_8g045555	71.111	90	25	1	1	89	63	152	1.28e-43	139
MsG0880043971.01.T02	MTR_8g045490	69.474	95	27	2	1	94	63	156	1.14e-42	136
MsG0880043971.01.T02	MTR_8g045400	67.368	95	29	2	1	94	63	156	3.04e-42	135
MsG0880043971.01.T02	MTR_8g045300	67.021	94	30	1	1	93	63	156	3.95e-42	135
MsG0880043971.01.T02	MTR_1g030810	40.659	91	51	2	1	88	62	152	1.17e-18	75.5
MsG0880043971.01.T02	MTR_8g012550	37.363	91	55	2	2	91	66	155	2.97e-17	72.0
MsG0880043971.01.T02	MTR_1g030820	38.462	91	53	2	1	88	62	152	3.57e-17	71.6
MsG0880043971.01.T02	MTR_1g031640	43.333	90	44	4	1	89	64	147	2.74e-12	58.9
MsG0880043971.01.T01	MTR_8g045695	93.617	94	6	0	5	98	63	156	4.14e-61	183
MsG0880043971.01.T01	MTR_8g045735	94.565	92	5	0	5	96	63	154	1.06e-60	182
MsG0880043971.01.T01	MTR_8g045665	87.234	94	12	0	5	98	63	156	1.03e-56	172
MsG0880043971.01.T01	MTR_8g045640	84.536	97	15	0	2	98	60	156	2.56e-55	169
MsG0880043971.01.T01	MTR_8g045560	83.505	97	16	0	2	98	60	156	4.93e-55	168
MsG0880043971.01.T01	MTR_8g045520	72.632	95	25	1	5	98	63	157	9.47e-46	144
MsG0880043971.01.T01	MTR_8g045570	74.444	90	22	1	5	93	63	152	4.34e-45	142
MsG0880043971.01.T01	MTR_8g045555	71.111	90	25	1	5	93	63	152	1.02e-43	139
MsG0880043971.01.T01	MTR_8g045490	69.474	95	27	2	5	98	63	156	7.33e-43	137
MsG0880043971.01.T01	MTR_8g045400	67.368	95	29	2	5	98	63	156	3.17e-42	135
MsG0880043971.01.T01	MTR_8g045300	67.021	94	30	1	5	97	63	156	3.68e-42	135
MsG0880043971.01.T01	MTR_1g030810	40.860	93	52	2	3	92	60	152	2.21e-19	77.4
MsG0880043971.01.T01	MTR_1g030820	38.298	94	55	2	2	92	59	152	6.67e-18	73.9
MsG0880043971.01.T01	MTR_8g012550	36.458	96	59	2	1	95	61	155	1.65e-17	72.8

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880043971.01.T02	AT1G70870	44.944	89	47	2	1	88	51	138	2.63e-18	74.3
MsG0880043971.01.T02	AT5G28010	33.696	92	59	2	1	90	75	166	1.15e-13	63.2
MsG0880043971.01.T02	AT5G28010	33.696	92	59	2	1	90	75	166	1.15e-13	63.2
MsG0880043971.01.T02	AT1G24020	37.209	86	52	2	4	88	67	151	5.16e-13	61.2
MsG0880043971.01.T02	AT1G24020	37.209	86	52	2	4	88	67	151	5.16e-13	61.2
MsG0880043971.01.T02	AT1G70850	35.870	92	57	2	1	90	225	316	6.53e-13	62.8
MsG0880043971.01.T02	AT1G70850	35.870	92	57	2	1	90	225	316	6.53e-13	62.8
MsG0880043971.01.T02	AT1G23120	34.091	88	56	2	1	87	59	145	1.56e-12	59.7
MsG0880043971.01.T02	AT5G28000	34.066	91	58	2	2	90	74	164	2.15e-12	59.7
MsG0880043971.01.T02	AT1G23120	34.091	88	56	2	1	87	109	195	3.19e-12	59.7
MsG0880043971.01.T02	AT1G70840	32.609	92	60	2	1	90	80	171	4.43e-12	58.9
MsG0880043971.01.T02	AT1G70890	33.696	92	59	2	1	90	67	158	9.25e-12	57.8
MsG0880043971.01.T02	AT1G70830	33.333	93	60	2	1	91	82	174	1.38e-11	59.3
MsG0880043971.01.T02	AT4G23670	34.444	90	57	2	1	89	63	151	4.21e-11	55.8
MsG0880043971.01.T01	AT1G70870	43.478	92	50	2	2	92	48	138	7.39e-19	75.9
MsG0880043971.01.T01	AT5G28010	33.333	96	62	2	1	94	71	166	4.13e-14	64.3
MsG0880043971.01.T01	AT5G28010	33.333	96	62	2	1	94	71	166	4.13e-14	64.3
MsG0880043971.01.T01	AT1G23120	33.696	92	59	2	1	91	55	145	2.76e-13	61.6
MsG0880043971.01.T01	AT1G24020	37.209	86	52	2	8	92	67	151	5.27e-13	61.2
MsG0880043971.01.T01	AT1G24020	37.209	86	52	2	8	92	67	151	5.27e-13	61.2
MsG0880043971.01.T01	AT1G23120	33.696	92	59	2	1	91	105	195	5.39e-13	62.0
MsG0880043971.01.T01	AT1G70850	35.417	96	60	2	1	94	221	316	8.47e-13	62.8
MsG0880043971.01.T01	AT1G70850	35.417	96	60	2	1	94	221	316	8.47e-13	62.8
MsG0880043971.01.T01	AT5G28000	33.333	96	62	2	1	94	69	164	1.07e-12	60.5
MsG0880043971.01.T01	AT1G70840	32.292	96	63	2	1	94	76	171	1.52e-12	60.5
MsG0880043971.01.T01	AT1G70830	32.990	97	63	2	1	95	78	174	5.47e-12	60.5
MsG0880043971.01.T01	AT1G70890	33.696	92	59	2	5	94	67	158	1.04e-11	57.8
MsG0880043971.01.T01	AT4G23670	34.783	92	58	2	3	93	61	151	1.45e-11	57.4
MsG0880043971.01.T01	AT1G70830	31.915	94	62	2	4	95	34	127	2.67e-11	58.5
MsG0880043971.01.T01	AT1G70830	32.292	96	63	2	1	94	193	288	5.99e-11	57.4
MsG0880043971.01.T01	AT2G01520	31.915	94	62	2	1	93	59	151	5.41e-11	55.8

Find 20 sgRNAs with CRISPR-Local

Find 19 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAGATAACTTTCAAGTTGTT+TGG	0.327904	8:-33609160	MsG0880043971.01.T01:CDS
ATGGAAGTTCTGCTGTTAGA+TGG	0.407851	8:-33609078	MsG0880043971.01.T01:CDS
TGGAGATATTGAAAACTATA+AGG	0.439418	8:-33609137	MsG0880043971.01.T01:CDS
AGGTAGTCCATCCAGCCATT+TGG	0.456227	8:+33609016	None:intergenic
AAAAGCACTAGAGATATTGA+TGG	0.477335	8:-33608989	MsG0880043971.01.T01:CDS
ATAACTTTCAAGTTGTTTGG+TGG	0.479625	8:-33609157	MsG0880043971.01.T01:CDS
TCATCTTGTCAAAGGGGAAA+AGG	0.504175	8:-33608966	MsG0880043971.01.T01:CDS
TTGATGGTCATCTTGTCAAA+GGG	0.506740	8:-33608973	MsG0880043971.01.T01:CDS
GAGGATATTGATACTCCAAA+TGG	0.508694	8:-33609031	MsG0880043971.01.T01:CDS
TAACATAACAACAGATGGTA+AGG	0.518649	8:-33609230	MsG0880043971.01.T01:CDS
ATTGATGGTCATCTTGTCAA+AGG	0.543582	8:-33608974	MsG0880043971.01.T01:CDS
ATATTGATACTCCAAATGGC+TGG	0.546067	8:-33609027	MsG0880043971.01.T01:CDS
GCCTCTAACATAACAACAGA+TGG	0.552849	8:-33609235	None:intergenic
TGATACTCCAAATGGCTGGA+TGG	0.568648	8:-33609023	MsG0880043971.01.T01:CDS
GAAGTGCTTAACAAGGCTGA+TGG	0.580204	8:-33609097	MsG0880043971.01.T01:CDS
AGGTACACACATGCAATGAG+AGG	0.626711	8:-33609210	MsG0880043971.01.T01:CDS
CATCTTAGAAGTGCTTAACA+AGG	0.644958	8:-33609104	MsG0880043971.01.T01:CDS
TGATGGTCATCTTGTCAAAG+GGG	0.652198	8:-33608972	MsG0880043971.01.T01:CDS
TGATTATGAGAAGAACAATG+AGG	0.681814	8:-33609050	MsG0880043971.01.T01:CDS
GGTACACACATGCAATGAGA+GGG	0.691358	8:-33609209	MsG0880043971.01.T01:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	TGGAGATATTGAAAACTATA+AGG	-	Chr8:33609047-33609066	MsG0880043971.01.T01:CDS	25.0%
!!!	AAGATAACTTTCAAGTTGTT+TGG	-	Chr8:33609024-33609043	MsG0880043971.01.T01:CDS	25.0%
	TGATTATGAGAAGAACAATG+AGG	-	Chr8:33609134-33609153	MsG0880043971.01.T01:CDS	30.0%
!!	AAAAGCACTAGAGATATTGA+TGG	-	Chr8:33609195-33609214	MsG0880043971.01.T01:CDS	30.0%
!!	ATAACTTTCAAGTTGTTTGG+TGG	-	Chr8:33609027-33609046	MsG0880043971.01.T01:CDS	30.0%
	ATATTGATACTCCAAATGGC+TGG	-	Chr8:33609157-33609176	MsG0880043971.01.T01:CDS	35.0%
	GAGGATATTGATACTCCAAA+TGG	-	Chr8:33609153-33609172	MsG0880043971.01.T01:CDS	35.0%
!	ATTGATGGTCATCTTGTCAA+AGG	-	Chr8:33609210-33609229	MsG0880043971.01.T01:CDS	35.0%
!	TATCTCTAGTGCTTTTGCTA+AGG	+	Chr8:33609191-33609210	None:intergenic	35.0%
!	TTGATGGTCATCTTGTCAAA+GGG	-	Chr8:33609211-33609230	MsG0880043971.01.T01:CDS	35.0%
!!	CATCTTAGAAGTGCTTAACA+AGG	-	Chr8:33609080-33609099	MsG0880043971.01.T01:CDS	35.0%
	ATGGAAGTTCTGCTGTTAGA+TGG	-	Chr8:33609106-33609125	MsG0880043971.01.T01:CDS	40.0%
	TCATCTTGTCAAAGGGGAAA+AGG	-	Chr8:33609218-33609237	MsG0880043971.01.T01:CDS	40.0%
	TGATGGTCATCTTGTCAAAG+GGG	-	Chr8:33609212-33609231	MsG0880043971.01.T01:CDS	40.0%
	AGGTACACACATGCAATGAG+AGG	-	Chr8:33608974-33608993	MsG0880043971.01.T01:CDS	45.0%
	GGTACACACATGCAATGAGA+GGG	-	Chr8:33608975-33608994	MsG0880043971.01.T01:CDS	45.0%
	TGATACTCCAAATGGCTGGA+TGG	-	Chr8:33609161-33609180	MsG0880043971.01.T01:CDS	45.0%
!	GAAGTGCTTAACAAGGCTGA+TGG	-	Chr8:33609087-33609106	MsG0880043971.01.T01:CDS	45.0%
	AGGTAGTCCATCCAGCCATT+TGG	+	Chr8:33609171-33609190	None:intergenic	50.0%

Chromosome	Type	Strat	End	Strand	Name
Chr8	gene	33608955	33609251	33608955	ID=MsG0880043971.01;Name=MsG0880043971.01
Chr8	mRNA	33608955	33609251	33608955	ID=MsG0880043971.01.T01;Parent=MsG0880043971.01;Name=MsG0880043971.01.T01;_AED=0.50;_eAED=0.50;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|98
Chr8	exon	33608955	33609251	33608955	ID=MsG0880043971.01.T01:exon:10223;Parent=MsG0880043971.01.T01
Chr8	CDS	33608955	33609251	33608955	ID=MsG0880043971.01.T01:cds;Parent=MsG0880043971.01.T01
Chr8	mRNA	33608955	33609239	33608955	ID=MsG0880043971.01.T02;Parent=MsG0880043971.01;Name=MsG0880043971.01.T02;_AED=0.50;_eAED=0.50;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|94
Chr8	exon	33608955	33609239	33608955	ID=MsG0880043971.01.T02:exon:10224;Parent=MsG0880043971.01.T02
Chr8	CDS	33608955	33609239	33608955	ID=MsG0880043971.01.T02:cds;Parent=MsG0880043971.01.T02

Gene Sequence

>MsG0880043971.01.T02

GATGGTAAGGTACACACATGCAATGAGAGGGTTGAAGAAGTTGATGAGCAAAACAAAAAGATAACTTTCAAGTTGTTTGGTGGAGATATTGAAAACTATAAGGTCTTTAAGCTCATCTTAGAAGTGCTTAACAAGGCTGATGGAAGTTCTGCTGTTAGATGGACTATTGATTATGAGAAGAACAATGAGGATATTGATACTCCAAATGGCTGGATGGACTACCTTAGCAAAAGCACTAGAGATATTGATGGTCATCTTGTCAAAGGGGAAAAGGTAGCTCTATAA

>MsG0880043971.01.T01

AACATAACAACAGATGGTAAGGTACACACATGCAATGAGAGGGTTGAAGAAGTTGATGAGCAAAACAAAAAGATAACTTTCAAGTTGTTTGGTGGAGATATTGAAAACTATAAGGTCTTTAAGCTCATCTTAGAAGTGCTTAACAAGGCTGATGGAAGTTCTGCTGTTAGATGGACTATTGATTATGAGAAGAACAATGAGGATATTGATACTCCAAATGGCTGGATGGACTACCTTAGCAAAAGCACTAGAGATATTGATGGTCATCTTGTCAAAGGGGAAAAGGTAGCTCTATAA

Protein sequence

>MsG0880043971.01.T02

DGKVHTCNERVEEVDEQNKKITFKLFGGDIENYKVFKLILEVLNKADGSSAVRWTIDYEKNNEDIDTPNGWMDYLSKSTRDIDGHLVKGEKVAL*

>MsG0880043971.01.T01

NITTDGKVHTCNERVEEVDEQNKKITFKLFGGDIENYKVFKLILEVLNKADGSSAVRWTIDYEKNNEDIDTPNGWMDYLSKSTRDIDGHLVKGEKVAL*