Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880043975.01.T01 | XP_013445246.1 | 93.617 | 94 | 6 | 0 | 16 | 109 | 63 | 156 | 1.26E-57 | 185 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880043975.01.T01 | P85524 | 40.426 | 94 | 54 | 2 | 11 | 103 | 58 | 150 | 1.58E-14 | 68.2 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880043975.01.T01 | I3SJ62 | 93.617 | 94 | 6 | 0 | 16 | 109 | 63 | 156 | 6.04e-58 | 185 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048461.01 | MsG0880043975.01 | 0.802703 | 4.947045e-49 | 2.439770e-46 |
| MsG0880043971.01 | MsG0880043975.01 | 0.921585 | 2.961824e-88 | 1.068469e-83 |
| MsG0880043975.01 | MsG0880043976.01 | 0.867139 | 1.704857e-65 | 5.888465e-62 |
| MsG0880043975.01 | MsG0880043992.01 | 0.871697 | 5.610743e-67 | 2.284609e-63 |
| MsG0880043975.01 | MsG0880043994.01 | 0.844944 | 5.524320e-59 | 9.062005e-56 |
| MsG0880043975.01 | MsG0880046027.01 | 0.813934 | 1.990293e-51 | 1.317310e-48 |
| MsG0880043975.01 | MsG0880046901.01 | 0.806450 | 8.177906e-50 | 4.440143e-47 |
| MsG0180005445.01 | MsG0880043975.01 | 0.827957 | 1.174365e-54 | 1.151041e-51 |
| MsG0880042520.01 | MsG0880043975.01 | 0.806049 | 9.934731e-50 | 5.339177e-47 |
| MsG0280010907.01 | MsG0880043975.01 | 0.801915 | 7.185930e-49 | 3.473485e-46 |
| MsG0680030447.01 | MsG0880043975.01 | 0.809215 | 2.112047e-50 | 1.232751e-47 |
| MsG0280008909.01 | MsG0880043975.01 | 0.800705 | 1.271341e-48 | 5.959743e-46 |
| MsG0780039443.01 | MsG0880043975.01 | 0.811344 | 7.337509e-51 | 4.530446e-48 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880043975.01.T01 | MTR_8g045695 | 93.617 | 94 | 6 | 0 | 16 | 109 | 63 | 156 | 1.53e-61 | 185 |
| MsG0880043975.01.T01 | MTR_8g045735 | 94.565 | 92 | 5 | 0 | 16 | 107 | 63 | 154 | 5.16e-61 | 184 |
| MsG0880043975.01.T01 | MTR_8g045665 | 87.234 | 94 | 12 | 0 | 16 | 109 | 63 | 156 | 4.80e-57 | 173 |
| MsG0880043975.01.T01 | MTR_8g045640 | 84.536 | 97 | 15 | 0 | 13 | 109 | 60 | 156 | 9.86e-56 | 170 |
| MsG0880043975.01.T01 | MTR_8g045560 | 86.170 | 94 | 13 | 0 | 16 | 109 | 63 | 156 | 2.61e-55 | 169 |
| MsG0880043975.01.T01 | MTR_8g045520 | 72.632 | 95 | 25 | 1 | 16 | 109 | 63 | 157 | 3.96e-46 | 146 |
| MsG0880043975.01.T01 | MTR_8g045570 | 74.444 | 90 | 22 | 1 | 16 | 104 | 63 | 152 | 3.58e-45 | 143 |
| MsG0880043975.01.T01 | MTR_8g045555 | 71.111 | 90 | 25 | 1 | 16 | 104 | 63 | 152 | 6.63e-44 | 140 |
| MsG0880043975.01.T01 | MTR_8g045490 | 69.474 | 95 | 27 | 2 | 16 | 109 | 63 | 156 | 4.64e-43 | 138 |
| MsG0880043975.01.T01 | MTR_8g045400 | 67.368 | 95 | 29 | 2 | 16 | 109 | 63 | 156 | 2.26e-42 | 136 |
| MsG0880043975.01.T01 | MTR_8g045300 | 67.021 | 94 | 30 | 1 | 16 | 108 | 63 | 156 | 3.20e-42 | 136 |
| MsG0880043975.01.T01 | MTR_1g030810 | 40.000 | 95 | 54 | 2 | 12 | 103 | 58 | 152 | 2.97e-19 | 77.8 |
| MsG0880043975.01.T01 | MTR_1g030820 | 37.895 | 95 | 56 | 2 | 12 | 103 | 58 | 152 | 5.33e-18 | 74.3 |
| MsG0880043975.01.T01 | MTR_8g012550 | 37.363 | 91 | 55 | 2 | 17 | 106 | 66 | 155 | 4.08e-17 | 72.4 |
| MsG0880043975.01.T01 | MTR_1g030840 | 38.235 | 102 | 56 | 3 | 7 | 101 | 54 | 155 | 3.71e-14 | 64.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880043975.01.T01 | AT1G70870 | 43.478 | 92 | 50 | 2 | 13 | 103 | 48 | 138 | 8.90e-19 | 76.3 |
| MsG0880043975.01.T01 | AT5G28010 | 33.696 | 92 | 59 | 2 | 16 | 105 | 75 | 166 | 2.60e-13 | 62.8 |
| MsG0880043975.01.T01 | AT5G28010 | 33.696 | 92 | 59 | 2 | 16 | 105 | 75 | 166 | 2.60e-13 | 62.8 |
| MsG0880043975.01.T01 | AT1G24020 | 37.209 | 86 | 52 | 2 | 19 | 103 | 67 | 151 | 6.90e-13 | 61.2 |
| MsG0880043975.01.T01 | AT1G24020 | 37.209 | 86 | 52 | 2 | 19 | 103 | 67 | 151 | 6.90e-13 | 61.2 |
| MsG0880043975.01.T01 | AT1G70850 | 35.870 | 92 | 57 | 2 | 16 | 105 | 225 | 316 | 1.43e-12 | 62.4 |
| MsG0880043975.01.T01 | AT1G70850 | 35.870 | 92 | 57 | 2 | 16 | 105 | 225 | 316 | 1.43e-12 | 62.4 |
| MsG0880043975.01.T01 | AT1G23120 | 34.091 | 88 | 56 | 2 | 16 | 102 | 59 | 145 | 1.89e-12 | 60.1 |
| MsG0880043975.01.T01 | AT1G23120 | 34.091 | 88 | 56 | 2 | 16 | 102 | 109 | 195 | 2.85e-12 | 60.5 |
| MsG0880043975.01.T01 | AT5G28000 | 34.066 | 91 | 58 | 2 | 17 | 105 | 74 | 164 | 3.99e-12 | 59.7 |
| MsG0880043975.01.T01 | AT1G70840 | 32.609 | 92 | 60 | 2 | 16 | 105 | 80 | 171 | 7.69e-12 | 58.9 |
| MsG0880043975.01.T01 | AT4G23670 | 34.783 | 92 | 58 | 2 | 14 | 104 | 61 | 151 | 2.45e-11 | 57.4 |
| MsG0880043975.01.T01 | AT1G70890 | 33.696 | 92 | 59 | 2 | 16 | 105 | 67 | 158 | 3.08e-11 | 57.0 |
| MsG0880043975.01.T01 | AT1G70830 | 33.333 | 93 | 60 | 2 | 16 | 106 | 82 | 174 | 4.40e-11 | 58.5 |
| MsG0880043975.01.T01 | AT1G70830 | 30.097 | 103 | 70 | 2 | 6 | 106 | 25 | 127 | 5.45e-11 | 57.8 |
Find 20 sgRNAs with CRISPR-Local
Find 21 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAGATAACTTTCAAGTTGTT+TGG | 0.327904 | 8:-33666056 | MsG0880043975.01.T01:CDS |
| ATGGAAGTTCTGCTGTTAGA+TGG | 0.407851 | 8:-33665974 | MsG0880043975.01.T01:CDS |
| TGGAGATATTGAAAACTATA+AGG | 0.439418 | 8:-33666033 | MsG0880043975.01.T01:CDS |
| AGGTAGTCCATCCAGCCATT+TGG | 0.456227 | 8:+33665912 | None:intergenic |
| AAAAGCACTAGAGATATTGA+TGG | 0.477335 | 8:-33665885 | MsG0880043975.01.T01:CDS |
| ATAACTTTCAAGTTGTTTGG+TGG | 0.479625 | 8:-33666053 | MsG0880043975.01.T01:CDS |
| ACAAATAACAACAGATGGTA+AGG | 0.489687 | 8:-33666126 | MsG0880043975.01.T01:CDS |
| TCATCTTGTCAAAGGGGAAA+AGG | 0.504175 | 8:-33665862 | MsG0880043975.01.T01:CDS |
| TTGATGGTCATCTTGTCAAA+GGG | 0.506740 | 8:-33665869 | MsG0880043975.01.T01:CDS |
| GAGGATATTGATACTCCAAA+TGG | 0.508694 | 8:-33665927 | MsG0880043975.01.T01:CDS |
| ATTGATGGTCATCTTGTCAA+AGG | 0.543582 | 8:-33665870 | MsG0880043975.01.T01:CDS |
| ATATTGATACTCCAAATGGC+TGG | 0.546067 | 8:-33665923 | MsG0880043975.01.T01:CDS |
| GAGCTACAAATAACAACAGA+TGG | 0.568082 | 8:-33666131 | MsG0880043975.01.T01:CDS |
| TGATACTCCAAATGGCTGGA+TGG | 0.568648 | 8:-33665919 | MsG0880043975.01.T01:CDS |
| GAAGTGCTTAACAAGGCTGA+TGG | 0.580204 | 8:-33665993 | MsG0880043975.01.T01:CDS |
| AGGTACACACATGCAATGAG+AGG | 0.626711 | 8:-33666106 | MsG0880043975.01.T01:CDS |
| CATCTTAGAAGTGCTTAACA+AGG | 0.644958 | 8:-33666000 | MsG0880043975.01.T01:CDS |
| TGATGGTCATCTTGTCAAAG+GGG | 0.652198 | 8:-33665868 | MsG0880043975.01.T01:CDS |
| TGATTATGAGAAGAACAATG+AGG | 0.681814 | 8:-33665946 | MsG0880043975.01.T01:CDS |
| GGTACACACATGCAATGAGA+GGG | 0.691358 | 8:-33666105 | MsG0880043975.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | TGGAGATATTGAAAACTATA+AGG | - | Chr8:33665976-33665995 | MsG0880043975.01.T01:CDS | 25.0% |
| !!! | AAGATAACTTTCAAGTTGTT+TGG | - | Chr8:33665953-33665972 | MsG0880043975.01.T01:CDS | 25.0% |
| ACAAATAACAACAGATGGTA+AGG | - | Chr8:33665883-33665902 | MsG0880043975.01.T01:CDS | 30.0% | |
| TGATTATGAGAAGAACAATG+AGG | - | Chr8:33666063-33666082 | MsG0880043975.01.T01:CDS | 30.0% | |
| !! | AAAAGCACTAGAGATATTGA+TGG | - | Chr8:33666124-33666143 | MsG0880043975.01.T01:CDS | 30.0% |
| !! | ATAACTTTCAAGTTGTTTGG+TGG | - | Chr8:33665956-33665975 | MsG0880043975.01.T01:CDS | 30.0% |
| ATATTGATACTCCAAATGGC+TGG | - | Chr8:33666086-33666105 | MsG0880043975.01.T01:CDS | 35.0% | |
| GAGCTACAAATAACAACAGA+TGG | - | Chr8:33665878-33665897 | MsG0880043975.01.T01:CDS | 35.0% | |
| GAGGATATTGATACTCCAAA+TGG | - | Chr8:33666082-33666101 | MsG0880043975.01.T01:CDS | 35.0% | |
| ! | ATTGATGGTCATCTTGTCAA+AGG | - | Chr8:33666139-33666158 | MsG0880043975.01.T01:CDS | 35.0% |
| ! | TATCTCTAGTGCTTTTGCTA+AGG | + | Chr8:33666120-33666139 | None:intergenic | 35.0% |
| ! | TTGATGGTCATCTTGTCAAA+GGG | - | Chr8:33666140-33666159 | MsG0880043975.01.T01:CDS | 35.0% |
| !! | CATCTTAGAAGTGCTTAACA+AGG | - | Chr8:33666009-33666028 | MsG0880043975.01.T01:CDS | 35.0% |
| ATGGAAGTTCTGCTGTTAGA+TGG | - | Chr8:33666035-33666054 | MsG0880043975.01.T01:CDS | 40.0% | |
| TCATCTTGTCAAAGGGGAAA+AGG | - | Chr8:33666147-33666166 | MsG0880043975.01.T01:CDS | 40.0% | |
| TGATGGTCATCTTGTCAAAG+GGG | - | Chr8:33666141-33666160 | MsG0880043975.01.T01:CDS | 40.0% | |
| AGGTACACACATGCAATGAG+AGG | - | Chr8:33665903-33665922 | MsG0880043975.01.T01:CDS | 45.0% | |
| GGTACACACATGCAATGAGA+GGG | - | Chr8:33665904-33665923 | MsG0880043975.01.T01:CDS | 45.0% | |
| TGATACTCCAAATGGCTGGA+TGG | - | Chr8:33666090-33666109 | MsG0880043975.01.T01:CDS | 45.0% | |
| ! | GAAGTGCTTAACAAGGCTGA+TGG | - | Chr8:33666016-33666035 | MsG0880043975.01.T01:CDS | 45.0% |
| AGGTAGTCCATCCAGCCATT+TGG | + | Chr8:33666100-33666119 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr8 | gene | 33665851 | 33666180 | 33665851 | ID=MsG0880043975.01;Name=MsG0880043975.01 |
| Chr8 | mRNA | 33665851 | 33666180 | 33665851 | ID=MsG0880043975.01.T01;Parent=MsG0880043975.01;Name=MsG0880043975.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|109 |
| Chr8 | exon | 33665851 | 33666180 | 33665851 | ID=MsG0880043975.01.T01:exon:10233;Parent=MsG0880043975.01.T01 |
| Chr8 | CDS | 33665851 | 33666180 | 33665851 | ID=MsG0880043975.01.T01:cds;Parent=MsG0880043975.01.T01 |
| Gene Sequence |
| Protein sequence |
