Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880044322.01.T01 | XP_003628500.1 | 86.207 | 174 | 24 | 0 | 14 | 187 | 30 | 203 | 1.09E-103 | 306 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880044322.01.T01 | G7LCV1 | 86.207 | 174 | 24 | 0 | 14 | 187 | 30 | 203 | 1.36E-106 | 306 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880044322.01.T01 | A0A2K3L4P4 | 67.797 | 177 | 54 | 3 | 14 | 187 | 11 | 187 | 8.93e-75 | 232 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0880044322.01 | MsG0880044644.01 | 0.822772 | 1.981259e-53 | 1.674196e-50 |
| MsG0480018664.01 | MsG0880044322.01 | 0.803023 | 4.248378e-49 | 2.112182e-46 |
| MsG0580026222.01 | MsG0880044322.01 | 0.824479 | 7.893905e-54 | 7.000127e-51 |
| MsG0580026631.01 | MsG0880044322.01 | 0.811710 | 6.111351e-51 | 3.810025e-48 |
| MsG0580027332.01 | MsG0880044322.01 | 0.831974 | 1.231336e-55 | 1.357685e-52 |
| MsG0580029381.01 | MsG0880044322.01 | 0.813008 | 3.181196e-51 | 2.053321e-48 |
| MsG0280009371.01 | MsG0880044322.01 | 0.801407 | 9.137383e-49 | 4.360208e-46 |
| MsG0780039282.01 | MsG0880044322.01 | 0.820951 | 5.229823e-53 | 4.197810e-50 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880044322.01.T01 | MTR_8g059790 | 86.207 | 174 | 24 | 0 | 14 | 187 | 30 | 203 | 1.32e-107 | 306 |
| MsG0880044322.01.T01 | MTR_8g461050 | 59.195 | 174 | 71 | 0 | 14 | 187 | 28 | 201 | 3.49e-75 | 224 |
| MsG0880044322.01.T01 | MTR_0100s0150 | 60.920 | 174 | 67 | 1 | 14 | 187 | 28 | 200 | 1.12e-70 | 213 |
| MsG0880044322.01.T01 | MTR_0302s0030 | 57.865 | 178 | 75 | 0 | 10 | 187 | 24 | 201 | 2.92e-70 | 212 |
| MsG0880044322.01.T01 | MTR_3g014870 | 57.778 | 180 | 73 | 2 | 10 | 187 | 24 | 202 | 1.40e-66 | 202 |
| MsG0880044322.01.T01 | MTR_0211s0080 | 59.195 | 174 | 67 | 1 | 14 | 187 | 28 | 197 | 3.40e-66 | 201 |
| MsG0880044322.01.T01 | MTR_3g014820 | 57.778 | 180 | 73 | 2 | 10 | 187 | 24 | 202 | 4.30e-66 | 201 |
| MsG0880044322.01.T01 | MTR_8g060550 | 55.172 | 174 | 76 | 2 | 14 | 187 | 28 | 199 | 5.09e-64 | 196 |
| MsG0880044322.01.T01 | MTR_8g461690 | 55.172 | 174 | 76 | 2 | 14 | 187 | 28 | 199 | 5.09e-64 | 196 |
| MsG0880044322.01.T01 | MTR_7g108815 | 59.091 | 154 | 61 | 2 | 10 | 163 | 24 | 175 | 7.04e-61 | 188 |
| MsG0880044322.01.T01 | MTR_0084s0030 | 54.598 | 174 | 79 | 0 | 14 | 187 | 28 | 201 | 1.02e-59 | 185 |
| MsG0880044322.01.T01 | MTR_2g073010 | 50.877 | 171 | 75 | 2 | 14 | 184 | 33 | 194 | 1.49e-53 | 169 |
| MsG0880044322.01.T01 | MTR_2g072980 | 43.195 | 169 | 77 | 5 | 13 | 176 | 32 | 186 | 3.67e-35 | 122 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 50 sgRNAs with CRISPR-Local
Find 54 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GATCATATGGTAACTTCTTT+AGG | 0.202488 | 8:-39955033 | MsG0880044322.01.T01:CDS |
| ATCACGACTTCGACGATGTT+AGG | 0.298935 | 8:-39955189 | MsG0880044322.01.T01:CDS |
| CCGACTGTGTCGAGACAAAC+TGG | 0.320641 | 8:+39955379 | None:intergenic |
| TATTCCTCGTCGCCTTCAAC+TGG | 0.432772 | 8:+39955352 | None:intergenic |
| AGTGCTACTAATGGATCATA+TGG | 0.432987 | 8:-39955046 | MsG0880044322.01.T01:CDS |
| GGAAGAGATAGTGCTACTAA+TGG | 0.447916 | 8:-39955055 | MsG0880044322.01.T01:CDS |
| GGTCTTGAGAACACTGGCTT+AGG | 0.457139 | 8:-39955250 | MsG0880044322.01.T01:CDS |
| TTCTCAAGACCAACATGTAA+AGG | 0.464201 | 8:+39955262 | None:intergenic |
| TCACGACTTCGACGATGTTA+GGG | 0.467925 | 8:-39955188 | MsG0880044322.01.T01:CDS |
| TTCATTTCTGCTGATCATAG+TGG | 0.471060 | 8:+39955291 | None:intergenic |
| CAATCAACAGAATGGAGATT+GGG | 0.482818 | 8:-39955118 | MsG0880044322.01.T01:CDS |
| GGAAGAAGGTTGATTATCAC+TGG | 0.495698 | 8:-39955076 | MsG0880044322.01.T01:CDS |
| GTACACAACTTGATGAAGCT+TGG | 0.498312 | 8:+39955140 | None:intergenic |
| AAGTCGTGATGGGGAGCAAA+AGG | 0.500088 | 8:+39955202 | None:intergenic |
| TCTCAAGACCAACATGTAAA+GGG | 0.503765 | 8:+39955263 | None:intergenic |
| GGGAGGGGAACCACCATCAA+GGG | 0.504188 | 8:+39954887 | None:intergenic |
| ACAATCAACAGAATGGAGAT+TGG | 0.508137 | 8:-39955119 | MsG0880044322.01.T01:CDS |
| AACATCGTCGAAGTCGTGAT+GGG | 0.515072 | 8:+39955192 | None:intergenic |
| ATTGTAGAGACCCCACTTGA+AGG | 0.515965 | 8:-39955010 | MsG0880044322.01.T01:CDS |
| AGGGAGGGGAACCACCATCA+AGG | 0.521691 | 8:+39954886 | None:intergenic |
| CCTTTGTTTGTGATAACTGA+CGG | 0.524254 | 8:+39955322 | None:intergenic |
| CACTGGCTTAGGACACGGTC+TGG | 0.532480 | 8:-39955239 | MsG0880044322.01.T01:CDS |
| CAACTAGGACATACCTCTGT+AGG | 0.534967 | 8:+39954965 | None:intergenic |
| CTTTCTGGAAACAAGAGGGA+GGG | 0.539553 | 8:+39954871 | None:intergenic |
| GAATCATGCCCTTTACATGT+TGG | 0.540282 | 8:-39955271 | MsG0880044322.01.T01:CDS |
| GTTGTGTACAATCAACAGAA+TGG | 0.542722 | 8:-39955126 | MsG0880044322.01.T01:CDS |
| TTTCTGGAAACAAGAGGGAG+GGG | 0.544355 | 8:+39954872 | None:intergenic |
| TAACATCGTCGAAGTCGTGA+TGG | 0.545399 | 8:+39955191 | None:intergenic |
| AAGATACTAAGAGTGGAAGA+AGG | 0.571477 | 8:-39955090 | MsG0880044322.01.T01:CDS |
| CCACATTCAAATCTACAACT+AGG | 0.573570 | 8:+39954950 | None:intergenic |
| TATTCTTTCTGGAAACAAGA+GGG | 0.578330 | 8:+39954867 | None:intergenic |
| TATTATACATACCTTCAAGT+GGG | 0.589842 | 8:+39954999 | None:intergenic |
| AAACTGGGCTATTGTTGTTG+TGG | 0.592731 | 8:+39955395 | None:intergenic |
| CCGTCAGTTATCACAAACAA+AGG | 0.604757 | 8:-39955322 | MsG0880044322.01.T01:CDS |
| ACAGTCGGAGAACCAGTTGA+AGG | 0.605474 | 8:-39955364 | MsG0880044322.01.T01:CDS |
| CATGTTGGTCTTGAGAACAC+TGG | 0.606261 | 8:-39955256 | MsG0880044322.01.T01:CDS |
| TTATTCTTTCTGGAAACAAG+AGG | 0.610786 | 8:+39954866 | None:intergenic |
| TGTTGATAATGCTGATGAGA+TGG | 0.611441 | 8:-39954924 | MsG0880044322.01.T01:CDS |
| ACATCGTCGAAGTCGTGATG+GGG | 0.622395 | 8:+39955193 | None:intergenic |
| GAGAACACTGGCTTAGGACA+CGG | 0.624273 | 8:-39955244 | MsG0880044322.01.T01:CDS |
| ATATTATACATACCTTCAAG+TGG | 0.635907 | 8:+39954998 | None:intergenic |
| CCTAGTTGTAGATTTGAATG+TGG | 0.636625 | 8:-39954950 | MsG0880044322.01.T01:CDS |
| TCTTTCTGGAAACAAGAGGG+AGG | 0.657842 | 8:+39954870 | None:intergenic |
| CGACTGTGTCGAGACAAACT+GGG | 0.662219 | 8:+39955380 | None:intergenic |
| CCAGTTTGTCTCGACACAGT+CGG | 0.662561 | 8:-39955379 | MsG0880044322.01.T01:CDS |
| TATACAGTGGTGTCCTACAG+AGG | 0.674639 | 8:-39954978 | MsG0880044322.01.T01:CDS |
| ATTATACATACCTTCAAGTG+GGG | 0.680213 | 8:+39955000 | None:intergenic |
| AGAACCAGTTGAAGGCGACG+AGG | 0.683558 | 8:-39955356 | MsG0880044322.01.T01:CDS |
| AAGGTATGTATAATATACAG+TGG | 0.689864 | 8:-39954991 | MsG0880044322.01.T01:CDS |
| TCAGTTATCACAAACAAAGG+AGG | 0.696848 | 8:-39955319 | MsG0880044322.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AAGGTATGTATAATATACAG+TGG | - | Chr8:39955277-39955296 | MsG0880044322.01.T01:CDS | 25.0% |
| ! | ATATTATACATACCTTCAAG+TGG | + | Chr8:39955273-39955292 | None:intergenic | 25.0% |
| ! | TATTATACATACCTTCAAGT+GGG | + | Chr8:39955272-39955291 | None:intergenic | 25.0% |
| ATTATACATACCTTCAAGTG+GGG | + | Chr8:39955271-39955290 | None:intergenic | 30.0% | |
| GATCATATGGTAACTTCTTT+AGG | - | Chr8:39955235-39955254 | MsG0880044322.01.T01:CDS | 30.0% | |
| ! | TATTCTTTCTGGAAACAAGA+GGG | + | Chr8:39955404-39955423 | None:intergenic | 30.0% |
| ! | TTATTCTTTCTGGAAACAAG+AGG | + | Chr8:39955405-39955424 | None:intergenic | 30.0% |
| AAGATACTAAGAGTGGAAGA+AGG | - | Chr8:39955178-39955197 | MsG0880044322.01.T01:CDS | 35.0% | |
| ACAATCAACAGAATGGAGAT+TGG | - | Chr8:39955149-39955168 | MsG0880044322.01.T01:CDS | 35.0% | |
| CAATCAACAGAATGGAGATT+GGG | - | Chr8:39955150-39955169 | MsG0880044322.01.T01:CDS | 35.0% | |
| CCACATTCAAATCTACAACT+AGG | + | Chr8:39955321-39955340 | None:intergenic | 35.0% | |
| CCTAGTTGTAGATTTGAATG+TGG | - | Chr8:39955318-39955337 | MsG0880044322.01.T01:CDS | 35.0% | |
| GGTGAAAAAGATACTAAGAG+TGG | - | Chr8:39955171-39955190 | MsG0880044322.01.T01:CDS | 35.0% | |
| GTTGTGTACAATCAACAGAA+TGG | - | Chr8:39955142-39955161 | MsG0880044322.01.T01:CDS | 35.0% | |
| TCAGTTATCACAAACAAAGG+AGG | - | Chr8:39954949-39954968 | MsG0880044322.01.T01:CDS | 35.0% | |
| TCTCAAGACCAACATGTAAA+GGG | + | Chr8:39955008-39955027 | None:intergenic | 35.0% | |
| TTCATTTCTGCTGATCATAG+TGG | + | Chr8:39954980-39954999 | None:intergenic | 35.0% | |
| TTCTCAAGACCAACATGTAA+AGG | + | Chr8:39955009-39955028 | None:intergenic | 35.0% | |
| ! | AGTGCTACTAATGGATCATA+TGG | - | Chr8:39955222-39955241 | MsG0880044322.01.T01:CDS | 35.0% |
| ! | ATGAGATGGTAAGATTTTGC+TGG | - | Chr8:39955358-39955377 | MsG0880044322.01.T01:CDS | 35.0% |
| ! | CCTTTGTTTGTGATAACTGA+CGG | + | Chr8:39954949-39954968 | None:intergenic | 35.0% |
| !! | TGTTGATAATGCTGATGAGA+TGG | - | Chr8:39955344-39955363 | MsG0880044322.01.T01:CDS | 35.0% |
| CCGTCAGTTATCACAAACAA+AGG | - | Chr8:39954946-39954965 | MsG0880044322.01.T01:CDS | 40.0% | |
| GAATCATGCCCTTTACATGT+TGG | - | Chr8:39954997-39955016 | MsG0880044322.01.T01:CDS | 40.0% | |
| GTACACAACTTGATGAAGCT+TGG | + | Chr8:39955131-39955150 | None:intergenic | 40.0% | |
| ! | AAACTGGGCTATTGTTGTTG+TGG | + | Chr8:39954876-39954895 | None:intergenic | 40.0% |
| ! | GGAAGAAGGTTGATTATCAC+TGG | - | Chr8:39955192-39955211 | MsG0880044322.01.T01:CDS | 40.0% |
| ! | GGAAGAGATAGTGCTACTAA+TGG | - | Chr8:39955213-39955232 | MsG0880044322.01.T01:CDS | 40.0% |
| AACATCGTCGAAGTCGTGAT+GGG | + | Chr8:39955079-39955098 | None:intergenic | 45.0% | |
| ATCACGACTTCGACGATGTT+AGG | - | Chr8:39955079-39955098 | MsG0880044322.01.T01:CDS | 45.0% | |
| ATTGTAGAGACCCCACTTGA+AGG | - | Chr8:39955258-39955277 | MsG0880044322.01.T01:CDS | 45.0% | |
| CAACTAGGACATACCTCTGT+AGG | + | Chr8:39955306-39955325 | None:intergenic | 45.0% | |
| TAACATCGTCGAAGTCGTGA+TGG | + | Chr8:39955080-39955099 | None:intergenic | 45.0% | |
| TATACAGTGGTGTCCTACAG+AGG | - | Chr8:39955290-39955309 | MsG0880044322.01.T01:CDS | 45.0% | |
| TCACGACTTCGACGATGTTA+GGG | - | Chr8:39955080-39955099 | MsG0880044322.01.T01:CDS | 45.0% | |
| ! | AAGATTTTGCTGGCCCTTGA+TGG | - | Chr8:39955368-39955387 | MsG0880044322.01.T01:CDS | 45.0% |
| ! | CTTTCTGGAAACAAGAGGGA+GGG | + | Chr8:39955400-39955419 | None:intergenic | 45.0% |
| ! | TCTTTCTGGAAACAAGAGGG+AGG | + | Chr8:39955401-39955420 | None:intergenic | 45.0% |
| ! | TTTCTGGAAACAAGAGGGAG+GGG | + | Chr8:39955399-39955418 | None:intergenic | 45.0% |
| !! | CATGTTGGTCTTGAGAACAC+TGG | - | Chr8:39955012-39955031 | MsG0880044322.01.T01:CDS | 45.0% |
| ACAGTCGGAGAACCAGTTGA+AGG | - | Chr8:39954904-39954923 | MsG0880044322.01.T01:CDS | 50.0% | |
| ACATCGTCGAAGTCGTGATG+GGG | + | Chr8:39955078-39955097 | None:intergenic | 50.0% | |
| CCAGTTTGTCTCGACACAGT+CGG | - | Chr8:39954889-39954908 | MsG0880044322.01.T01:CDS | 50.0% | |
| CGACTGTGTCGAGACAAACT+GGG | + | Chr8:39954891-39954910 | None:intergenic | 50.0% | |
| TATTCCTCGTCGCCTTCAAC+TGG | + | Chr8:39954919-39954938 | None:intergenic | 50.0% | |
| ! | AAGTCGTGATGGGGAGCAAA+AGG | + | Chr8:39955069-39955088 | None:intergenic | 50.0% |
| ! | ATTTTGCTGGCCCTTGATGG+TGG | - | Chr8:39955371-39955390 | MsG0880044322.01.T01:CDS | 50.0% |
| !! | GAGAACACTGGCTTAGGACA+CGG | - | Chr8:39955024-39955043 | MsG0880044322.01.T01:CDS | 50.0% |
| !! | GGTCTTGAGAACACTGGCTT+AGG | - | Chr8:39955018-39955037 | MsG0880044322.01.T01:CDS | 50.0% |
| AGAACCAGTTGAAGGCGACG+AGG | - | Chr8:39954912-39954931 | MsG0880044322.01.T01:CDS | 55.0% | |
| CCGACTGTGTCGAGACAAAC+TGG | + | Chr8:39954892-39954911 | None:intergenic | 55.0% | |
| AGGGAGGGGAACCACCATCA+AGG | + | Chr8:39955385-39955404 | None:intergenic | 60.0% | |
| GGGAGGGGAACCACCATCAA+GGG | + | Chr8:39955384-39955403 | None:intergenic | 60.0% | |
| !! | CACTGGCTTAGGACACGGTC+TGG | - | Chr8:39955029-39955048 | MsG0880044322.01.T01:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr8 | gene | 39954859 | 39955431 | 39954859 | ID=MsG0880044322.01;Name=MsG0880044322.01 |
| Chr8 | mRNA | 39954859 | 39955431 | 39954859 | ID=MsG0880044322.01.T01;Parent=MsG0880044322.01;Name=MsG0880044322.01.T01;_AED=0.40;_eAED=0.40;_QI=0|-1|0|1|-1|1|1|0|190 |
| Chr8 | exon | 39954859 | 39955431 | 39954859 | ID=MsG0880044322.01.T01:exon:3192;Parent=MsG0880044322.01.T01 |
| Chr8 | CDS | 39954859 | 39955431 | 39954859 | ID=MsG0880044322.01.T01:cds;Parent=MsG0880044322.01.T01 |
| Gene Sequence |
| Protein sequence |
