AlfalfaGEDB | MsG0880044443.01

M. sativa cultivar ZhongmuNo.1 / MsG0880044443.01

KEGG: K02887
GO: GO:0003735 GO:0005622 GO:0005840 GO:0006412 GO:0019843
PFAM: PF00453

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880044443.01.T01	YP_009656614.1	95.833	120	5	0	1	120	1	120	6.89E-76	230
MsG0880044443.01.T02	AWW91855.1	97.297	37	1	0	1	37	84	120	1.60E-15	73.9

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880044443.01.T01	Q9BBR0	81.667	120	20	1	1	118	1	120	1.24E-63	192
MsG0880044443.01.T02	Q9BBR0	91.429	35	3	0	1	35	86	120	6.78E-16	67.8

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880044443.01.T01	A0A0F6NLU4	95.833	120	5	1	120	1	120	3.29e-76	230
MsG0880044443.01.T01	A0A0X8GJX4	95.833	120	5	1	120	1	120	3.29e-76	230
MsG0880044443.01.T02	A0A4P8F1L6	97.297	37	1	1	37	84	120	7.64e-16	73.9

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0380014037.01	MsG0880044443.01	0.803537	3.324898e-49	1.675165e-46
MsG0680033966.01	MsG0880044443.01	0.856919	2.323418e-62	5.634614e-59

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880044443.01.T01	MTR_4g034720	91.525	118	10	1	118	1	118	4.73e-74	216
MsG0880044443.01.T01	MTR_7g085940	91.011	89	8	1	89	1	89	1.06e-53	163
MsG0880044443.01.T02	MTR_4g034720	91.429	35	3	1	35	84	118	1.69e-16	66.6

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880044443.01.T01	ATCG00660	63.866	119	41	1	1	119	1	117	1.41e-50	156

Find 11 sgRNAs with CRISPR-Local

Find 16 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GCACAAATAGCTATATTAAA+AGG	0.182382	8:+42183957	MsG0880044443.01.T01:CDS
AGCTTTGGTTTCGGCTCATC+AGG	0.349867	8:+42183782	MsG0880044443.01.T01:CDS
GCAACAGAAAATAAAAGCTT+TGG	0.373336	8:+42183767	MsG0880044443.01.T01:CDS
GAAAATAAAAGCTTTGGTTT+CGG	0.379754	8:+42183773	MsG0880044443.01.T01:CDS
CACAAATAGCTATATTAAAA+GGG	0.466532	8:+42183958	MsG0880044443.01.T01:CDS
GAGGATCTATCGCTCGAAAA+CGG	0.476329	8:+42183676	MsG0880044443.01.T01:CDS
CGGCTCATCAGGATAGAGAT+AGG	0.510072	8:+42183793	MsG0880044443.01.T01:CDS
TTATTTACATCAAGCTTTCG+CGG	0.568648	8:+42183714	MsG0880044443.01.T01:CDS
GATAGATCCTCGTTTAACTC+TGG	0.576409	8:-42183664	None:intergenic
GATCTATCGCTCGAAAACGG+AGG	0.661022	8:+42183679	MsG0880044443.01.T01:CDS
TCAATGACCAGAGTTAAACG+AGG	0.757581	8:+42183657	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	CACAAATAGCTATATTAAAA+GGG	+	Chr8:42183958-42183977	MsG0880044443.01.T01:CDS	20.0%
!!!	AGTATACGTTGTTTTTATTT+TGG	-	Chr8:42183872-42183891	None:intergenic	20.0%
!!!	GTATACGTTGTTTTTATTTT+GGG	-	Chr8:42183871-42183890	None:intergenic	20.0%
!!!	TTATTTTTTTATGATCTCGT+TGG	-	Chr8:42184003-42184022	None:intergenic	20.0%
!	GCACAAATAGCTATATTAAA+AGG	+	Chr8:42183957-42183976	MsG0880044443.01.T01:CDS	25.0%
!!	GAAAATAAAAGCTTTGGTTT+CGG	+	Chr8:42183773-42183792	MsG0880044443.01.T01:CDS	25.0%
	AGGATAGAGATAGGAAAAAA+AGG	+	Chr8:42183802-42183821	MsG0880044443.01.T01:CDS	30.0%
	GCAACAGAAAATAAAAGCTT+TGG	+	Chr8:42183767-42183786	MsG0880044443.01.T01:CDS	30.0%
	GGATAGAGATAGGAAAAAAA+GGG	+	Chr8:42183803-42183822	MsG0880044443.01.T01:CDS	30.0%
	TTATTTACATCAAGCTTTCG+CGG	+	Chr8:42183714-42183733	MsG0880044443.01.T01:CDS	30.0%
	GATAGATCCTCGTTTAACTC+TGG	-	Chr8:42183667-42183686	None:intergenic	40.0%
!	AAAGGGATTTTCGCAGTTTG+TGG	+	Chr8:42183820-42183839	MsG0880044443.01.T01:CDS	40.0%
	GAGGATCTATCGCTCGAAAA+CGG	+	Chr8:42183676-42183695	MsG0880044443.01.T01:CDS	45.0%
	CGGCTCATCAGGATAGAGAT+AGG	+	Chr8:42183793-42183812	MsG0880044443.01.T01:CDS	50.0%
	GATCTATCGCTCGAAAACGG+AGG	+	Chr8:42183679-42183698	MsG0880044443.01.T01:CDS	50.0%
!	AGCTTTGGTTTCGGCTCATC+AGG	+	Chr8:42183782-42183801	MsG0880044443.01.T01:CDS	50.0%

Chromosome	Type	Strat	End	Strand	Name
Chr8	gene	42183660	42184022	42183660	ID=MsG0880044443.01;Name=MsG0880044443.01
Chr8	mRNA	42183660	42184022	42183660	ID=MsG0880044443.01.T01;Parent=MsG0880044443.01;Name=MsG0880044443.01.T01;_AED=0.47;_eAED=0.47;_QI=0\|-1\|0\|1\|-1\|0\|1\|0\|120
Chr8	exon	42183660	42184022	42183660	ID=MsG0880044443.01.T01:exon:39614;Parent=MsG0880044443.01.T01
Chr8	CDS	42183660	42184022	42183660	ID=MsG0880044443.01.T01:cds;Parent=MsG0880044443.01.T01
Chr8	mRNA	42183909	42184022	42183909	ID=MsG0880044443.01.T02;Parent=MsG0880044443.01;Name=MsG0880044443.01.T02;_AED=0.49;_eAED=0.49;_QI=0\|-1\|0\|1\|-1\|0\|1\|0\|37
Chr8	exon	42183909	42184022	42183909	ID=MsG0880044443.01.T02:exon:39615;Parent=MsG0880044443.01.T02
Chr8	CDS	42183909	42184022	42183909	ID=MsG0880044443.01.T02:cds;Parent=MsG0880044443.01.T02

Gene Sequence

>MsG0880044443.01.T01

ATGACCAGAGTTAAACGAGGATCTATCGCTCGAAAACGGAGGACAAAAATTCGTTTATTTACATCAAGCTTTCGCGGAGCTCATTCAAAACTTACTCGAACTATTTCGCAACAGAAAATAAAAGCTTTGGTTTCGGCTCATCAGGATAGAGATAGGAAAAAAAGGGATTTTCGCAGTTTGTGGATCAGTCGAATAAACGCAATAATTTCCCAAAATAAAAACAACGTATACTGTATTTATATTAAATTAATGTATAATCTGTACAAGAGTCAATTGCTTCTTAATCGTAAAATAGTTGCACAAATAGCTATATTAAAAGGGAATTGCATTTTTATGATTGCCAACGAGATCATAAAAAAATAA

>MsG0880044443.01.T02

ATGTATAATCTGTACAAGAGTCAATTGCTTCTTAATCGTAAAATAGTTGCACAAATAGCTATATTAAAAGGGAATTGCATTTTTATGATTGCCAACGAGATCATAAAAAAATAA

Protein sequence

>MsG0880044443.01.T01

MTRVKRGSIARKRRTKIRLFTSSFRGAHSKLTRTISQQKIKALVSAHQDRDRKKRDFRSLWISRINAIISQNKNNVYCIYIKLMYNLYKSQLLLNRKIVAQIAILKGNCIFMIANEIIKK*

>MsG0880044443.01.T02

MYNLYKSQLLLNRKIVAQIAILKGNCIFMIANEIIKK*