AlfalfaGEDB | MsG0680033966.01

M. sativa cultivar ZhongmuNo.1 / MsG0680033966.01

KEGG: K02887
GO: GO:0003735 GO:0005622 GO:0005840 GO:0006412 GO:0019843
PFAM: PF00453

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0680033966.01.T01	YP_009141633.1	97.5	120	3	0	1	120	1	120	8.38E-77	233
MsG0680033966.01.T02	AWW91855.1	97.297	37	1	0	1	37	84	120	2.88E-15	73.6

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0680033966.01.T01	Q9BBR0	82.5	120	19	1	1	118	1	120	5.14E-64	193
MsG0680033966.01.T02	Q9BBR0	91.429	35	3	0	1	35	86	120	1.30E-15	67

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0680033966.01.T01	A0A0F6NLU4	97.500	120	3	0	1	120	1	120	4.00e-77	233
MsG0680033966.01.T02	A0A2Z4KH10	97.297	37	1	0	1	37	84	120	1.38e-15	73.6

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080049078.01	MsG0680033966.01	0.852998	3.194134e-61	6.799668e-58
MsG0080049079.01	MsG0680033966.01	0.845814	3.210717e-59	5.416696e-56
MsG0180000502.01	MsG0680033966.01	0.802030	6.808225e-49	3.300433e-46
MsG0180004937.01	MsG0680033966.01	0.840557	8.092331e-58	1.156972e-54
MsG0180004987.01	MsG0680033966.01	0.829048	6.402275e-55	6.477196e-52
MsG0180005568.01	MsG0680033966.01	0.821489	3.929827e-53	3.201647e-50
MsG0280010974.01	MsG0680033966.01	0.806295	8.816782e-50	4.768129e-47
MsG0380012443.01	MsG0680033966.01	0.802788	4.751635e-49	2.348511e-46
MsG0380013927.01	MsG0680033966.01	0.834358	3.139070e-56	3.716391e-53
MsG0380013933.01	MsG0680033966.01	0.830600	2.680195e-55	2.837223e-52
MsG0380013939.01	MsG0680033966.01	0.837801	4.193890e-57	5.509553e-54
MsG0380013955.01	MsG0680033966.01	0.859279	4.615327e-63	1.212248e-59
MsG0380013958.01	MsG0680033966.01	0.869644	2.653344e-66	1.003052e-62
MsG0380013965.01	MsG0680033966.01	0.852231	5.286556e-61	1.097388e-57
MsG0380013970.01	MsG0680033966.01	0.864240	1.401727e-64	4.370829e-61
MsG0380013980.01	MsG0680033966.01	0.862748	4.069311e-64	1.204797e-60
MsG0380013992.01	MsG0680033966.01	0.817220	3.691630e-52	2.672436e-49
MsG0380013996.01	MsG0680033966.01	0.849632	2.854591e-60	5.445959e-57
MsG0380014000.01	MsG0680033966.01	0.810867	9.309315e-51	5.676397e-48
MsG0380014007.01	MsG0680033966.01	0.852087	5.809326e-61	1.200440e-57
MsG0380014009.01	MsG0680033966.01	0.801871	7.339853e-49	3.543999e-46
MsG0380014012.01	MsG0680033966.01	0.838192	3.327520e-57	4.423261e-54
MsG0380014013.01	MsG0680033966.01	0.833547	5.007834e-56	5.786213e-53
MsG0380014022.01	MsG0680033966.01	0.860888	1.508753e-63	4.188173e-60
MsG0380014030.01	MsG0680033966.01	0.800570	1.354381e-48	6.327519e-46
MsG0380014037.01	MsG0680033966.01	0.873742	1.161676e-67	5.096965e-64
MsG0380014038.01	MsG0680033966.01	0.844055	9.582620e-59	1.528737e-55
MsG0380014042.01	MsG0680033966.01	0.804934	1.701738e-49	8.887885e-47
MsG0380014048.01	MsG0680033966.01	0.875645	2.616770e-68	1.231390e-64
MsG0380014049.01	MsG0680033966.01	0.805815	1.112479e-49	5.943040e-47
MsG0380014053.01	MsG0680033966.01	0.824220	9.080816e-54	7.993438e-51
MsG0380014055.01	MsG0680033966.01	0.802424	5.647382e-49	2.765527e-46
MsG0380014056.01	MsG0680033966.01	0.810422	1.161899e-50	7.000508e-48
MsG0680033224.01	MsG0680033966.01	0.800521	1.386030e-48	6.467372e-46
MsG0680033966.01	MsG0680035308.01	0.802080	6.646226e-49	3.226111e-46
MsG0680033966.01	MsG0780036387.01	0.807096	5.971635e-50	3.296588e-47
MsG0680033966.01	MsG0780037705.01	0.828306	9.673827e-55	9.578113e-52
MsG0680033966.01	MsG0780037915.01	0.818760	1.656887e-52	1.251347e-49
MsG0680033966.01	MsG0780037946.01	0.823048	1.708471e-53	1.455024e-50
MsG0680033966.01	MsG0780039707.01	0.800170	1.634341e-48	7.558142e-46
MsG0680033966.01	MsG0780039975.01	0.807841	4.151260e-50	2.336501e-47
MsG0680033966.01	MsG0780040314.01	0.825391	4.807939e-54	4.376468e-51
MsG0680033966.01	MsG0780040315.01	0.808577	2.892735e-50	1.659987e-47
MsG0680033966.01	MsG0780040350.01	0.812777	3.574745e-51	2.293120e-48
MsG0680033966.01	MsG0780041004.01	0.807901	4.030946e-50	2.272268e-47
MsG0680033966.01	MsG0780041022.01	0.812348	4.435814e-51	2.813652e-48
MsG0680033966.01	MsG0880042006.01	0.805800	1.120690e-49	5.984534e-47
MsG0680033966.01	MsG0880043541.01	0.817494	3.202397e-52	2.335827e-49
MsG0680033966.01	MsG0880044443.01	0.856919	2.323418e-62	5.634614e-59
MsG0680033966.01	MsG0880044884.01	0.807774	4.288985e-50	2.409851e-47
MsG0680033966.01	MsG0880045283.01	0.840039	1.105034e-57	1.554973e-54
MsG0680033966.01	MsG0880045412.01	0.806848	6.739129e-50	3.696640e-47
MsG0680033966.01	MsG0880046288.01	0.807302	5.400742e-50	2.997641e-47
MsG0680033966.01	MsG0880047605.01	0.813579	2.382984e-51	1.562189e-48
MsG0480018983.01	MsG0680033966.01	0.809636	1.715618e-50	1.012690e-47
MsG0480021420.01	MsG0680033966.01	0.802623	5.138726e-49	2.529083e-46
MsG0480022804.01	MsG0680033966.01	0.806021	1.007158e-49	5.408858e-47
MsG0480023505.01	MsG0680033966.01	0.812239	4.686757e-51	2.964061e-48
MsG0380015898.01	MsG0680033966.01	0.802680	5.001370e-49	2.465038e-46
MsG0380016751.01	MsG0680033966.01	0.821375	4.174742e-53	3.390369e-50
MsG0380016762.01	MsG0680033966.01	0.848287	6.747567e-60	1.232308e-56
MsG0480018089.01	MsG0680033966.01	0.829211	5.843789e-55	5.941779e-52
MsG0480018324.01	MsG0680033966.01	0.803994	2.672483e-49	1.362371e-46
MsG0580026430.01	MsG0680033966.01	0.814562	1.446111e-51	9.736173e-49
MsG0580027098.01	MsG0680033966.01	0.802024	6.825819e-49	3.308514e-46
MsG0580027254.01	MsG0680033966.01	0.814886	1.225825e-51	8.324737e-49
MsG0580028101.01	MsG0680033966.01	0.816683	4.874138e-52	3.476795e-49
MsG0580028231.01	MsG0680033966.01	0.833100	6.474573e-56	7.381718e-53
MsG0580028853.01	MsG0680033966.01	0.839953	1.164012e-57	1.633431e-54
MsG0280007041.01	MsG0680033966.01	0.841657	4.161516e-58	6.156201e-55
MsG0280007042.01	MsG0680033966.01	0.806282	8.871205e-50	4.795993e-47
MsG0280010198.01	MsG0680033966.01	0.806831	6.794238e-50	3.725150e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MsG0680033966.01.T01	MTR_4g034720	93.220	118	8	1	118	1	118	5.27e-75	218
MsG0680033966.01.T01	MTR_7g085940	93.258	89	6	1	89	1	89	6.38e-55	166
MsG0680033966.01.T02	MTR_4g034720	91.429	35	3	1	35	84	118	2.65e-16	66.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0680033966.01.T01	ATCG00660	64.706	119	40	1	1	119	1	117	8.14e-51	157

Find 12 sgRNAs with CRISPR-Local

Find 17 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GCACAAATAGCTATATTAAA+AGG	0.182382	6:+77576362	MsG0680033966.01.T01:CDS
GCAACAGAAAATAAAAGCTT+TGG	0.365321	6:+77576172	MsG0680033966.01.T01:CDS
AGCTTTGGTTTCGGCTCATC+GGG	0.366647	6:+77576187	MsG0680033966.01.T01:CDS
GAAAATAAAAGCTTTGGTTT+CGG	0.379754	6:+77576178	MsG0680033966.01.T01:CDS
CACAAATAGCTATATTAAAA+GGG	0.466532	6:+77576363	MsG0680033966.01.T01:CDS
CGGCTCATCGGGATAGAGAT+AGG	0.471858	6:+77576198	MsG0680033966.01.T01:CDS
GAGGATCTATCGCTCGAAAA+CGG	0.476329	6:+77576081	MsG0680033966.01.T01:CDS
AAGCTTTGGTTTCGGCTCAT+CGG	0.491966	6:+77576186	MsG0680033966.01.T01:CDS
TTATTTACATCAAGCTTTCG+CGG	0.568648	6:+77576119	MsG0680033966.01.T01:CDS
GATAGATCCTCGTTTAACTC+TGG	0.576409	6:-77576069	None:intergenic
GATCTATCGCTCGAAAACGG+AGG	0.661022	6:+77576084	MsG0680033966.01.T01:CDS
TCAATGACCAGAGTTAAACG+AGG	0.757581	6:+77576062	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	CACAAATAGCTATATTAAAA+GGG	+	Chr6:77576363-77576382	MsG0680033966.01.T01:CDS	20.0%
!!!	AGTATACGTTGTTTTTATTT+TGG	-	Chr6:77576277-77576296	None:intergenic	20.0%
!!!	GTATACGTTGTTTTTATTTT+GGG	-	Chr6:77576276-77576295	None:intergenic	20.0%
!!!	TTATTTTTTTATGATCTCGT+TGG	-	Chr6:77576408-77576427	None:intergenic	20.0%
!	GCACAAATAGCTATATTAAA+AGG	+	Chr6:77576362-77576381	MsG0680033966.01.T01:CDS	25.0%
!!	GAAAATAAAAGCTTTGGTTT+CGG	+	Chr6:77576178-77576197	MsG0680033966.01.T01:CDS	25.0%
	GCAACAGAAAATAAAAGCTT+TGG	+	Chr6:77576172-77576191	MsG0680033966.01.T01:CDS	30.0%
	GGATAGAGATAGGAAAAAAA+GGG	+	Chr6:77576208-77576227	MsG0680033966.01.T01:CDS	30.0%
	TTATTTACATCAAGCTTTCG+CGG	+	Chr6:77576119-77576138	MsG0680033966.01.T01:CDS	30.0%
	GGGATAGAGATAGGAAAAAA+AGG	+	Chr6:77576207-77576226	MsG0680033966.01.T01:CDS	35.0%
	GATAGATCCTCGTTTAACTC+TGG	-	Chr6:77576072-77576091	None:intergenic	40.0%
!	AAAGGGATTTTCGCAGTTTG+TGG	+	Chr6:77576225-77576244	MsG0680033966.01.T01:CDS	40.0%
	GAGGATCTATCGCTCGAAAA+CGG	+	Chr6:77576081-77576100	MsG0680033966.01.T01:CDS	45.0%
!	AAGCTTTGGTTTCGGCTCAT+CGG	+	Chr6:77576186-77576205	MsG0680033966.01.T01:CDS	45.0%
	GATCTATCGCTCGAAAACGG+AGG	+	Chr6:77576084-77576103	MsG0680033966.01.T01:CDS	50.0%
!	AGCTTTGGTTTCGGCTCATC+GGG	+	Chr6:77576187-77576206	MsG0680033966.01.T01:CDS	50.0%
	CGGCTCATCGGGATAGAGAT+AGG	+	Chr6:77576198-77576217	MsG0680033966.01.T01:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
Chr6	gene	77576065	77576427	77576065	ID=MsG0680033966.01;Name=MsG0680033966.01
Chr6	mRNA	77576065	77576427	77576065	ID=MsG0680033966.01.T01;Parent=MsG0680033966.01;Name=MsG0680033966.01.T01;_AED=0.50;_eAED=0.50;_QI=0\|-1\|0\|1\|-1\|0\|1\|0\|120
Chr6	exon	77576065	77576427	77576065	ID=MsG0680033966.01.T01:exon:619;Parent=MsG0680033966.01.T01
Chr6	CDS	77576065	77576427	77576065	ID=MsG0680033966.01.T01:cds;Parent=MsG0680033966.01.T01
Chr6	mRNA	77576314	77576427	77576314	ID=MsG0680033966.01.T02;Parent=MsG0680033966.01;Name=MsG0680033966.01.T02;_AED=0.50;_eAED=0.50;_QI=0\|-1\|0\|1\|-1\|0\|1\|0\|37
Chr6	exon	77576314	77576427	77576314	ID=MsG0680033966.01.T02:exon:620;Parent=MsG0680033966.01.T02
Chr6	CDS	77576314	77576427	77576314	ID=MsG0680033966.01.T02:cds;Parent=MsG0680033966.01.T02

Gene Sequence

>MsG0680033966.01.T01

ATGACCAGAGTTAAACGAGGATCTATCGCTCGAAAACGGAGGACAAAAATTCGTTTATTTACATCAAGCTTTCGCGGAGCTCATTCAAAACTTACTCGAACTATTTTGCAACAGAAAATAAAAGCTTTGGTTTCGGCTCATCGGGATAGAGATAGGAAAAAAAGGGATTTTCGCAGTTTGTGGATCAGTCGAATAAACGCAATAATTGCCCAAAATAAAAACAACGTATACTGTATTTATAGTAAATTAATGTATAATCTGTACAAGAGTCAATTGCTTCTTAATCGTAAAACAGTTGCACAAATAGCTATATTAAAAGGGAATTGTCTTTTTATGATTGCCAACGAGATCATAAAAAAATAA

>MsG0680033966.01.T02

ATGTATAATCTGTACAAGAGTCAATTGCTTCTTAATCGTAAAACAGTTGCACAAATAGCTATATTAAAAGGGAATTGTCTTTTTATGATTGCCAACGAGATCATAAAAAAATAA

Protein sequence

>MsG0680033966.01.T01

MTRVKRGSIARKRRTKIRLFTSSFRGAHSKLTRTILQQKIKALVSAHRDRDRKKRDFRSLWISRINAIIAQNKNNVYCIYSKLMYNLYKSQLLLNRKTVAQIAILKGNCLFMIANEIIKK*

>MsG0680033966.01.T02

MYNLYKSQLLLNRKTVAQIAILKGNCLFMIANEIIKK*