Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880044846.01.T01 | XP_013445813.1 | 92.079 | 101 | 7 | 1 | 17 | 116 | 31 | 131 | 8.77E-59 | 187 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880044846.01.T01 | A0A072TQR1 | 92.079 | 101 | 7 | 1 | 17 | 116 | 31 | 131 | 4.19e-59 | 187 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0480019247.01 | MsG0880044846.01 | 0.857152 | 1.982314e-62 | 4.846253e-59 |
| MsG0480023087.01 | MsG0880044846.01 | 0.806989 | 6.290689e-50 | 3.463129e-47 |
| MsG0380017935.01 | MsG0880044846.01 | 0.806595 | 7.620784e-50 | 4.152864e-47 |
| MsG0280010227.01 | MsG0880044846.01 | 0.829231 | 5.777494e-55 | 5.877757e-52 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880044846.01.T01 | MTR_8g469260 | 92.079 | 101 | 7 | 1 | 17 | 116 | 31 | 131 | 1.06e-62 | 187 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 20 sgRNAs with CRISPR-Local
Find 25 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AATGGACGGCGATGTCTTTA+AGG | 0.172459 | 8:+50153982 | MsG0880044846.01.T01:CDS |
| CGCTTCATGTTATTCCATAT+TGG | 0.183390 | 8:+50153704 | None:intergenic |
| CAAGAAACAGCTTAATAAAA+AGG | 0.299718 | 8:+50153838 | MsG0880044846.01.T01:CDS |
| GCTTCATGTTATTCCATATT+GGG | 0.305547 | 8:+50153705 | None:intergenic |
| AGGCATTCCTCGTCCCAATA+TGG | 0.331832 | 8:-50153718 | None:intergenic |
| TTGCGACTCTTCAACAAGTT+TGG | 0.357116 | 8:-50153945 | None:intergenic |
| GCAACAGCCTCCAAACAAAA+TGG | 0.363156 | 8:+50153964 | MsG0880044846.01.T01:CDS |
| ATCATTACTATGCTCATTGC+AGG | 0.377944 | 8:+50153746 | MsG0880044846.01.T01:CDS |
| ATGGGAGGAGAGTGTTTATT+CGG | 0.386938 | 8:+50154026 | MsG0880044846.01.T01:CDS |
| GGTATATTTATCAATCCTTC+AGG | 0.395994 | 8:-50153794 | None:intergenic |
| AGCTGTTTCTTGGTCTTCAA+TGG | 0.399678 | 8:-50153827 | None:intergenic |
| TTCTTGGTCTTCAATGGTGT+TGG | 0.407736 | 8:-50153821 | None:intergenic |
| GTCTTCAATGGTGTTGGCAT+CGG | 0.449431 | 8:-50153815 | None:intergenic |
| ATGGACGGCGATGTCTTTAA+GGG | 0.460589 | 8:+50153983 | MsG0880044846.01.T01:CDS |
| TAGAAAGCAGGTAGATAAGA+TGG | 0.505727 | 8:+50153769 | MsG0880044846.01.T01:CDS |
| GAGCATAGTAATGATATTTG+AGG | 0.515992 | 8:-50153738 | None:intergenic |
| TGTTATTCCATATTGGGACG+AGG | 0.586587 | 8:+50153711 | MsG0880044846.01.T01:CDS |
| GCTCATTGCAGGTAGAAAGC+AGG | 0.591706 | 8:+50153757 | MsG0880044846.01.T01:CDS |
| CAGCCTCCAAACAAAATGGA+CGG | 0.632337 | 8:+50153968 | MsG0880044846.01.T01:CDS |
| GTAGATAAGATGGTGCCTGA+AGG | 0.644164 | 8:+50153779 | MsG0880044846.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATTCAATTTTGAAACTAAAG+TGG | + | Chr8:50153871-50153890 | MsG0880044846.01.T01:CDS | 20.0% |
| ! | ATTAATCTTCTGCAGAAAAA+TGG | + | Chr8:50154007-50154026 | MsG0880044846.01.T01:CDS | 25.0% |
| ! | CAAGAAACAGCTTAATAAAA+AGG | + | Chr8:50153838-50153857 | MsG0880044846.01.T01:CDS | 25.0% |
| ! | TTAATCTTCTGCAGAAAAAT+GGG | + | Chr8:50154008-50154027 | MsG0880044846.01.T01:CDS | 25.0% |
| !!! | CTTTTTATTAAGCTGTTTCT+TGG | - | Chr8:50153840-50153859 | None:intergenic | 25.0% |
| GAGCATAGTAATGATATTTG+AGG | - | Chr8:50153741-50153760 | None:intergenic | 30.0% | |
| GGTATATTTATCAATCCTTC+AGG | - | Chr8:50153797-50153816 | None:intergenic | 30.0% | |
| ATCATTACTATGCTCATTGC+AGG | + | Chr8:50153746-50153765 | MsG0880044846.01.T01:CDS | 35.0% | |
| TAGAAAGCAGGTAGATAAGA+TGG | + | Chr8:50153769-50153788 | MsG0880044846.01.T01:CDS | 35.0% | |
| ATCTTCTGCAGAAAAATGGG+AGG | + | Chr8:50154011-50154030 | MsG0880044846.01.T01:CDS | 40.0% | |
| TGTTATTCCATATTGGGACG+AGG | + | Chr8:50153711-50153730 | MsG0880044846.01.T01:CDS | 40.0% | |
| TTGCGACTCTTCAACAAGTT+TGG | - | Chr8:50153948-50153967 | None:intergenic | 40.0% | |
| ! | TTCTTGGTCTTCAATGGTGT+TGG | - | Chr8:50153824-50153843 | None:intergenic | 40.0% |
| !! | AGCTGTTTCTTGGTCTTCAA+TGG | - | Chr8:50153830-50153849 | None:intergenic | 40.0% |
| !! | ATGGGAGGAGAGTGTTTATT+CGG | + | Chr8:50154026-50154045 | MsG0880044846.01.T01:CDS | 40.0% |
| AATGGACGGCGATGTCTTTA+AGG | + | Chr8:50153982-50154001 | MsG0880044846.01.T01:CDS | 45.0% | |
| ATGGACGGCGATGTCTTTAA+GGG | + | Chr8:50153983-50154002 | MsG0880044846.01.T01:CDS | 45.0% | |
| CAGCCTCCAAACAAAATGGA+CGG | + | Chr8:50153968-50153987 | MsG0880044846.01.T01:CDS | 45.0% | |
| GCAACAGCCTCCAAACAAAA+TGG | + | Chr8:50153964-50153983 | MsG0880044846.01.T01:CDS | 45.0% | |
| GTAGATAAGATGGTGCCTGA+AGG | + | Chr8:50153779-50153798 | MsG0880044846.01.T01:CDS | 45.0% | |
| ! | ACATCGCCGTCCATTTTGTT+TGG | - | Chr8:50153977-50153996 | None:intergenic | 45.0% |
| !! | GTCTTCAATGGTGTTGGCAT+CGG | - | Chr8:50153818-50153837 | None:intergenic | 45.0% |
| AGGCATTCCTCGTCCCAATA+TGG | - | Chr8:50153721-50153740 | None:intergenic | 50.0% | |
| GCTCATTGCAGGTAGAAAGC+AGG | + | Chr8:50153757-50153776 | MsG0880044846.01.T01:CDS | 50.0% | |
| !! | TCGCCGTCCATTTTGTTTGG+AGG | - | Chr8:50153974-50153993 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr8 | gene | 50153710 | 50154060 | 50153710 | ID=MsG0880044846.01;Name=MsG0880044846.01 |
| Chr8 | mRNA | 50153710 | 50154060 | 50153710 | ID=MsG0880044846.01.T01;Parent=MsG0880044846.01;Name=MsG0880044846.01.T01;_AED=0.46;_eAED=0.46;_QI=0|-1|0|1|-1|1|1|0|116 |
| Chr8 | exon | 50153710 | 50154060 | 50153710 | ID=MsG0880044846.01.T01:exon:21067;Parent=MsG0880044846.01.T01 |
| Chr8 | CDS | 50153710 | 50154060 | 50153710 | ID=MsG0880044846.01.T01:cds;Parent=MsG0880044846.01.T01 |
| Gene Sequence |
| Protein sequence |
