Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880044968.01.T01 | XP_013457654.1 | 95.556 | 270 | 12 | 0 | 1 | 270 | 1 | 270 | 6.57E-176 | 499 |
| MsG0880044968.01.T02 | XP_013457654.1 | 95.556 | 270 | 12 | 0 | 1 | 270 | 1 | 270 | 6.57E-176 | 499 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880044968.01.T01 | A0A072V0M0 | 95.556 | 270 | 12 | 0 | 1 | 270 | 1 | 270 | 3.14e-176 | 499 |
| MsG0880044968.01.T01 | A0A396IHQ4 | 95.556 | 270 | 12 | 0 | 1 | 270 | 51 | 320 | 1.65e-175 | 499 |
| MsG0880044968.01.T02 | A0A072V0M0 | 95.556 | 270 | 12 | 0 | 1 | 270 | 1 | 270 | 3.14e-176 | 499 |
| MsG0880044968.01.T02 | A0A396IHQ4 | 95.556 | 270 | 12 | 0 | 1 | 270 | 51 | 320 | 1.65e-175 | 499 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080047831.01 | MsG0880044968.01 | -0.845341 | 4.315128e-59 | 7.168882e-56 |
| MsG0080047967.01 | MsG0880044968.01 | 0.882241 | 1.228873e-70 | 7.429468e-67 |
| MsG0080048036.01 | MsG0880044968.01 | -0.818662 | 1.743827e-52 | 1.313349e-49 |
| MsG0080048059.01 | MsG0880044968.01 | 0.907736 | 3.670652e-81 | 6.597076e-77 |
| MsG0080048111.01 | MsG0880044968.01 | -0.820700 | 5.973262e-53 | 4.760579e-50 |
| MsG0080048202.01 | MsG0880044968.01 | -0.826646 | 2.420237e-54 | 2.283186e-51 |
| MsG0080048256.01 | MsG0880044968.01 | -0.815789 | 7.720440e-52 | 5.374683e-49 |
| MsG0080048274.01 | MsG0880044968.01 | 0.816349 | 5.790966e-52 | 4.093538e-49 |
| MsG0080048347.01 | MsG0880044968.01 | -0.806497 | 7.992831e-50 | 4.344832e-47 |
| MsG0080048486.01 | MsG0880044968.01 | -0.816893 | 4.372807e-52 | 3.137326e-49 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880044968.01.T01 | MTR_4g102440 | 95.556 | 270 | 12 | 0 | 1 | 270 | 1 | 270 | 7.96e-180 | 499 |
| MsG0880044968.01.T01 | MTR_5g011220 | 76.793 | 237 | 50 | 3 | 36 | 270 | 29 | 262 | 5.24e-129 | 370 |
| MsG0880044968.01.T02 | MTR_4g102440 | 95.556 | 270 | 12 | 0 | 1 | 270 | 1 | 270 | 7.96e-180 | 499 |
| MsG0880044968.01.T02 | MTR_5g011220 | 76.793 | 237 | 50 | 3 | 36 | 270 | 29 | 262 | 5.24e-129 | 370 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880044968.01.T01 | AT4G22890 | 79.741 | 232 | 42 | 3 | 39 | 270 | 45 | 271 | 9.81e-131 | 374 |
| MsG0880044968.01.T01 | AT4G22890 | 79.741 | 232 | 42 | 3 | 39 | 270 | 45 | 271 | 1.42e-130 | 374 |
| MsG0880044968.01.T01 | AT4G22890 | 79.741 | 232 | 42 | 3 | 39 | 270 | 45 | 271 | 1.42e-130 | 374 |
| MsG0880044968.01.T01 | AT4G22890 | 79.310 | 232 | 40 | 3 | 39 | 270 | 45 | 268 | 7.75e-130 | 372 |
| MsG0880044968.01.T01 | AT4G22890 | 78.879 | 232 | 42 | 3 | 39 | 270 | 45 | 269 | 1.10e-128 | 370 |
| MsG0880044968.01.T01 | AT4G11960 | 73.684 | 266 | 64 | 4 | 7 | 271 | 1 | 261 | 1.16e-128 | 369 |
| MsG0880044968.01.T01 | AT4G11960 | 84.729 | 203 | 30 | 1 | 68 | 270 | 42 | 243 | 4.55e-123 | 354 |
| MsG0880044968.01.T02 | AT4G22890 | 79.741 | 232 | 42 | 3 | 39 | 270 | 45 | 271 | 9.81e-131 | 374 |
| MsG0880044968.01.T02 | AT4G22890 | 79.741 | 232 | 42 | 3 | 39 | 270 | 45 | 271 | 1.42e-130 | 374 |
| MsG0880044968.01.T02 | AT4G22890 | 79.741 | 232 | 42 | 3 | 39 | 270 | 45 | 271 | 1.42e-130 | 374 |
Find 86 sgRNAs with CRISPR-Local
Find 301 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAACCAAATCGAATCCTGC+CGG | 0.604286 | 8:+52244090 | None:intergenic |
| AAAGCCACTGCAGATCAACA+AGG | 0.689088 | 8:-52243440 | MsG0880044968.01.T01:intron |
| AAATGAGGTGAGACAGTAAA+TGG | 0.264956 | 8:-52243853 | MsG0880044968.01.T01:five_prime_UTR |
| AAGACAGTTGGCAACCGGCT+AGG | 0.549811 | 8:+52243766 | None:intergenic |
| AAGGCAATGAGAACGTGTGG+AGG | 0.623604 | 8:+52243721 | None:intergenic |
| AATCTAGCGTTGATATCCAA+CGG | 0.645227 | 8:-52244057 | MsG0880044968.01.T01:five_prime_UTR |
| AATGAGGTGAGACAGTAAAT+GGG | 0.469355 | 8:-52243852 | MsG0880044968.01.T01:five_prime_UTR |
| AATGGAATAAAGGGAGTTTG+GGG | 0.668538 | 8:+52243542 | None:intergenic |
| ACAGGCGGCAACGGAGGAGA+AGG | 0.535875 | 8:-52244228 | MsG0880044968.01.T01:intron |
| ACGTGGCGGGAGTTGAAGTG+AGG | 0.680548 | 8:+52243497 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAAATGAAACTAAAAGGCTC+CGG | + | Chr8:52244134-52244153 | None:intergenic | 30.0% | |
| ACTATACTGTCAATTTCAAG+TGG | - | Chr8:52244355-52244374 | MsG0880044968.01.T01:five_prime_UTR | 30.0% | |
| ACTTCTGCCAAATATATGAT+TGG | - | Chr8:52243015-52243034 | MsG0880044968.01.T01:intron | 30.0% | |
| AGTAGAATGTTGACATCAAA+TGG | + | Chr8:52242968-52242987 | None:intergenic | 30.0% | |
| ATAGAGCTAGATATACAATG+AGG | + | Chr8:52244178-52244197 | None:intergenic | 30.0% | |
| ATCATATATTTGGCAGAAGT+AGG | + | Chr8:52243015-52243034 | None:intergenic | 30.0% | |
| ATCATCAAATGGTAATCCAT+TGG | + | Chr8:52243946-52243965 | None:intergenic | 30.0% | |
| CAAATCCAGTTATATCATCA+AGG | + | Chr8:52243677-52243696 | None:intergenic | 30.0% | |
| CAAGTAGTGACAAGAAATAT+AGG | - | Chr8:52242629-52242648 | MsG0880044968.01.T01:intron | 30.0% | |
| GAAACAGAAGAAAAAGAGAA+TGG | + | Chr8:52241045-52241064 | None:intergenic | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr8 | gene | 52240155 | 52244433 | 52240155 | ID=MsG0880044968.01;Name=MsG0880044968.01 |
| Chr8 | mRNA | 52240155 | 52244416 | 52240155 | ID=MsG0880044968.01.T01;Parent=MsG0880044968.01;Name=MsG0880044968.01.T01;_AED=0.49;_eAED=0.52;_QI=750|0.88|0.9|1|0.88|0.8|10|0|308 |
| Chr8 | exon | 52243441 | 52244246 | 52243441 | ID=MsG0880044968.01.T01:exon:22325;Parent=MsG0880044968.01.T01 |
| Chr8 | exon | 52244292 | 52244416 | 52244292 | ID=MsG0880044968.01.T01:exon:22324;Parent=MsG0880044968.01.T01 |
| Chr8 | five_prime_UTR | 52244292 | 52244416 | 52244292 | ID=MsG0880044968.01.T01:five_prime_utr;Parent=MsG0880044968.01.T01 |
| Chr8 | five_prime_UTR | 52243622 | 52244246 | 52243622 | ID=MsG0880044968.01.T01:five_prime_utr;Parent=MsG0880044968.01.T01 |
| Chr8 | CDS | 52243441 | 52243621 | 52243441 | ID=MsG0880044968.01.T01:cds;Parent=MsG0880044968.01.T01 |
| Chr8 | CDS | 52243064 | 52243191 | 52243064 | ID=MsG0880044968.01.T01:cds;Parent=MsG0880044968.01.T01 |
| Chr8 | CDS | 52242732 | 52242828 | 52242732 | ID=MsG0880044968.01.T01:cds;Parent=MsG0880044968.01.T01 |
| Chr8 | CDS | 52242525 | 52242625 | 52242525 | ID=MsG0880044968.01.T01:cds;Parent=MsG0880044968.01.T01 |
| Chr8 | CDS | 52242177 | 52242233 | 52242177 | ID=MsG0880044968.01.T01:cds;Parent=MsG0880044968.01.T01 |
| Chr8 | CDS | 52241710 | 52241786 | 52241710 | ID=MsG0880044968.01.T01:cds;Parent=MsG0880044968.01.T01 |
| Chr8 | CDS | 52240872 | 52240923 | 52240872 | ID=MsG0880044968.01.T01:cds;Parent=MsG0880044968.01.T01 |
| Chr8 | CDS | 52240350 | 52240460 | 52240350 | ID=MsG0880044968.01.T01:cds;Parent=MsG0880044968.01.T01 |
| Chr8 | CDS | 52240155 | 52240277 | 52240155 | ID=MsG0880044968.01.T01:cds;Parent=MsG0880044968.01.T01 |
| Chr8 | mRNA | 52240155 | 52244433 | 52240155 | ID=MsG0880044968.01.T02;Parent=MsG0880044968.01;Name=MsG0880044968.01.T02;_AED=0.49;_eAED=0.52;_QI=812|0.87|0.88|1|1|1|9|0|308 |
| Chr8 | exon | 52243441 | 52244433 | 52243441 | ID=MsG0880044968.01.T02:exon:22334;Parent=MsG0880044968.01.T02 |
| Chr8 | five_prime_UTR | 52243622 | 52244433 | 52243622 | ID=MsG0880044968.01.T02:five_prime_utr;Parent=MsG0880044968.01.T02 |
| Chr8 | CDS | 52243441 | 52243621 | 52243441 | ID=MsG0880044968.01.T02:cds;Parent=MsG0880044968.01.T02 |
| Chr8 | CDS | 52243064 | 52243191 | 52243064 | ID=MsG0880044968.01.T02:cds;Parent=MsG0880044968.01.T02 |
| Chr8 | CDS | 52242732 | 52242828 | 52242732 | ID=MsG0880044968.01.T02:cds;Parent=MsG0880044968.01.T02 |
| Chr8 | CDS | 52242525 | 52242625 | 52242525 | ID=MsG0880044968.01.T02:cds;Parent=MsG0880044968.01.T02 |
| Chr8 | CDS | 52242177 | 52242233 | 52242177 | ID=MsG0880044968.01.T02:cds;Parent=MsG0880044968.01.T02 |
| Chr8 | CDS | 52241710 | 52241786 | 52241710 | ID=MsG0880044968.01.T02:cds;Parent=MsG0880044968.01.T02 |
| Chr8 | CDS | 52240872 | 52240923 | 52240872 | ID=MsG0880044968.01.T02:cds;Parent=MsG0880044968.01.T02 |
| Chr8 | CDS | 52240350 | 52240460 | 52240350 | ID=MsG0880044968.01.T02:cds;Parent=MsG0880044968.01.T02 |
| Chr8 | CDS | 52240155 | 52240277 | 52240155 | ID=MsG0880044968.01.T02:cds;Parent=MsG0880044968.01.T02 |
| Gene Sequence |
>MsG0880044968.01.T02 ATGTTCACTGCTACCAAAGTAGCCTTCACTTTAACACCCCCTCCTCCATGTTCTGCACCCCAAACTCCCTTTATTCCATTCTCTTTTTCTTCTGTTTCTCGTCCTCACTTCAACTCCCGCCACGTATGCCTTCGCCGCAGATTACTCCTACTTTCGCCTAAAGCCACTGCAGATCAACAAGGGAATGCTGGAGATTTTGACGGAGATGCTGTTATTGATACTAATGTCCTCCCATATTGTAGCATAGATCAAAAAAAGGAGAAGAAATCCGTTGGTGAACTTGAGCAAGAATTTCTTCAAGCACTCCAATCATTCTATTATGAGGGAAAGGCTATTATGTCAAATGAAGAATTTGATAATCTCAAGGAGGAACTTATGTGGGAAGGAAGCAGTGTTGTCATGCTAAGTTCGGATGAACAAAAATTTCTTGAAGCTTCTATGGCTTATGTATCTGGAAATCCAATTTTGAGCGACAAAGAGTATGATAAGTTGAAACTGAATCTAAAGAGAGAAGGGAGTGAAATTGTTGTTGAGGGTCCAAGGTGCAGCCTTCGAAGTAAAAAGGTTTACAGTGACCTGTCTGTTGACTATTTAAAGATGTTCCTGCTGAACGTCCCAGCCACTGTGGTTGCATTAGGATTATTCTTCTTCCTTGATGATATAACTGGATTTGAGATCACTTATTTGCTAGAGCTGCCGGAGCCTTTTAGTTTCATTTTCACTTGGTTTGCGGCTGTTCCCCTCATTGTATATCTAGCTCTATCACTAACAAGGGCCATTATCAAAGATTTTGTGATTTTAAAGGTCCCTGTCCAAACTGCGGAACTGAAAATACCTCCTTCTTTGGGACTATACTGTCAATTTCAAGTGGTGGTTCCAACAACAAAGTCAAATGCTCTGAGTAAGTACAAAAATACTGCTGACTAA >MsG0880044968.01.T01 ATGTTCACTGCTACCAAAGTAGCCTTCACTTTAACACCCCCTCCTCCATGTTCTGCACCCCAAACTCCCTTTATTCCATTCTCTTTTTCTTCTGTTTCTCGTCCTCACTTCAACTCCCGCCACGTATGCCTTCGCCGCAGATTACTCCTACTTTCGCCTAAAGCCACTGCAGATCAACAAGGGAATGCTGGAGATTTTGACGGAGATGCTGTTATTGATACTAATGTCCTCCCATATTGTAGCATAGATCAAAAAAAGGAGAAGAAATCCGTTGGTGAACTTGAGCAAGAATTTCTTCAAGCACTCCAATCATTCTATTATGAGGGAAAGGCTATTATGTCAAATGAAGAATTTGATAATCTCAAGGAGGAACTTATGTGGGAAGGAAGCAGTGTTGTCATGCTAAGTTCGGATGAACAAAAATTTCTTGAAGCTTCTATGGCTTATGTATCTGGAAATCCAATTTTGAGCGACAAAGAGTATGATAAGTTGAAACTGAATCTAAAGAGAGAAGGGAGTGAAATTGTTGTTGAGGGTCCAAGGTGCAGCCTTCGAAGTAAAAAGGTTTACAGTGACCTGTCTGTTGACTATTTAAAGATGTTCCTGCTGAACGTCCCAGCCACTGTGGTTGCATTAGGATTATTCTTCTTCCTTGATGATATAACTGGATTTGAGATCACTTATTTGCTAGAGCTGCCGGAGCCTTTTAGTTTCATTTTCACTTGGTTTGCGGCTGTTCCCCTCATTGTATATCTAGCTCTATCACTAACAAGGGCCATTATCAAAGATTTTGTGATTTTAAAGGTCCCTGTCCAAACTGCGGAACTGAAAATACCTCCTTCTTTGGGACTATACTGTCAATTTCAAGTGGTGGTTCCAACAACAAAGTCAAATGCTCTGAGTAAGTACAAAAATACTGCTGACTAA |
| Protein sequence |
>MsG0880044968.01.T02 MFTATKVAFTLTPPPPCSAPQTPFIPFSFSSVSRPHFNSRHVCLRRRLLLLSPKATADQQGNAGDFDGDAVIDTNVLPYCSIDQKKEKKSVGELEQEFLQALQSFYYEGKAIMSNEEFDNLKEELMWEGSSVVMLSSDEQKFLEASMAYVSGNPILSDKEYDKLKLNLKREGSEIVVEGPRCSLRSKKVYSDLSVDYLKMFLLNVPATVVALGLFFFLDDITGFEITYLLELPEPFSFIFTWFAAVPLIVYLALSLTRAIIKDFVILKVPVQTAELKIPPSLGLYCQFQVVVPTTKSNALSKYKNTAD* >MsG0880044968.01.T01 MFTATKVAFTLTPPPPCSAPQTPFIPFSFSSVSRPHFNSRHVCLRRRLLLLSPKATADQQGNAGDFDGDAVIDTNVLPYCSIDQKKEKKSVGELEQEFLQALQSFYYEGKAIMSNEEFDNLKEELMWEGSSVVMLSSDEQKFLEASMAYVSGNPILSDKEYDKLKLNLKREGSEIVVEGPRCSLRSKKVYSDLSVDYLKMFLLNVPATVVALGLFFFLDDITGFEITYLLELPEPFSFIFTWFAAVPLIVYLALSLTRAIIKDFVILKVPVQTAELKIPPSLGLYCQFQVVVPTTKSNALSKYKNTAD* |
