Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880045286.01.T01 | AET03424.1 | 99.099 | 111 | 1 | 0 | 1 | 111 | 38 | 148 | 2.96E-73 | 224 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880045286.01.T01 | Q9LR33 | 79.279 | 111 | 21 | 1 | 1 | 111 | 38 | 146 | 1.73E-58 | 179 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880045286.01.T01 | G7LJC8 | 99.099 | 111 | 1 | 0 | 1 | 111 | 38 | 148 | 1.42e-73 | 224 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0480021293.01 | MsG0880045286.01 | 0.819806 | 9.572871e-53 | 7.442943e-50 |
| MsG0280006760.01 | MsG0880045286.01 | 0.804032 | 2.623952e-49 | 1.338918e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880045286.01.T01 | MTR_8g069460 | 99.099 | 111 | 1 | 0 | 1 | 111 | 38 | 148 | 3.59e-77 | 224 |
| MsG0880045286.01.T01 | MTR_5g033090 | 81.081 | 111 | 20 | 1 | 1 | 111 | 38 | 147 | 1.10e-60 | 182 |
| MsG0880045286.01.T01 | MTR_3g077050 | 80.180 | 111 | 21 | 1 | 1 | 111 | 38 | 147 | 2.89e-60 | 181 |
| MsG0880045286.01.T01 | MTR_4g119380 | 43.243 | 111 | 23 | 3 | 1 | 111 | 122 | 192 | 4.24e-16 | 70.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880045286.01.T01 | AT1G23290 | 79.279 | 111 | 21 | 1 | 1 | 111 | 38 | 146 | 1.77e-59 | 179 |
| MsG0880045286.01.T01 | AT1G70600 | 78.378 | 111 | 22 | 1 | 1 | 111 | 38 | 146 | 4.85e-59 | 178 |
| MsG0880045286.01.T01 | AT1G12960 | 52.000 | 100 | 18 | 2 | 13 | 110 | 29 | 100 | 1.11e-23 | 87.8 |
Find 32 sgRNAs with CRISPR-Local
Find 32 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TACGCAGTTTGGGTTCTTTA+AGG | 0.253757 | 8:+56919485 | MsG0880045286.01.T01:CDS |
| TTTGGGTTCTTTAAGGTTCT+TGG | 0.257988 | 8:+56919492 | MsG0880045286.01.T01:CDS |
| GATTGCTGAGAAGAAGATTA+AGG | 0.341259 | 8:+56919572 | MsG0880045286.01.T01:CDS |
| ACAACAACACTCTGATTCTT+CGG | 0.371380 | 8:-56919528 | None:intergenic |
| GTGATTGATGTTACGCAGTT+TGG | 0.378054 | 8:+56919474 | MsG0880045286.01.T01:CDS |
| TTGGGTTCTTTAAGGTTCTT+GGG | 0.390951 | 8:+56919493 | MsG0880045286.01.T01:CDS |
| TTCTTTAAGGTTCTTGGGAA+AGG | 0.411341 | 8:+56919498 | MsG0880045286.01.T01:CDS |
| TGATTGATGTTACGCAGTTT+GGG | 0.440404 | 8:+56919475 | MsG0880045286.01.T01:CDS |
| CCCACTTTGCCGAAGTAACC+AGG | 0.472874 | 8:-56919327 | None:intergenic |
| TGTTGTTGTTAAAGCGAAGC+TGG | 0.489814 | 8:+56919542 | MsG0880045286.01.T01:CDS |
| GCCTTGTCCTTCACTTCTTG+AGG | 0.494689 | 8:-56919426 | None:intergenic |
| CCGTGAACATCGATACTCTC+TGG | 0.537090 | 8:+56919394 | MsG0880045286.01.T01:CDS |
| TGTCGAAGAGGATGCGGTGG+TGG | 0.540799 | 8:-56919296 | None:intergenic |
| CCAGAGAGTATCGATGTTCA+CGG | 0.544302 | 8:-56919394 | None:intergenic |
| CCCTGGTTACTTCGGCAAAG+TGG | 0.546405 | 8:+56919326 | MsG0880045286.01.T01:CDS |
| CCTGGTTACTTCGGCAAAGT+GGG | 0.551584 | 8:+56919327 | MsG0880045286.01.T01:CDS |
| AAGTATCACCCTGGTTACTT+CGG | 0.566184 | 8:+56919318 | MsG0880045286.01.T01:CDS |
| CGATGTTCACGGTGGGACAG+TGG | 0.575870 | 8:-56919383 | None:intergenic |
| GAGAAGAAGATTAAGGAAGC+TGG | 0.577905 | 8:+56919579 | MsG0880045286.01.T01:CDS |
| GATACTTGTCGAAGAGGATG+CGG | 0.579398 | 8:-56919302 | None:intergenic |
| TGGGACAGTGGAATTTGTTG+CGG | 0.598138 | 8:-56919371 | None:intergenic |
| TCTTCTACCTCAAGAAGTGA+AGG | 0.601537 | 8:+56919419 | MsG0880045286.01.T01:CDS |
| CAGGGTGATACTTGTCGAAG+AGG | 0.610306 | 8:-56919308 | None:intergenic |
| GGACAAGGCATCAAAGTCGA+AGG | 0.615129 | 8:+56919440 | MsG0880045286.01.T01:CDS |
| AACTGCGTAACATCAATCAC+AGG | 0.626930 | 8:-56919471 | None:intergenic |
| AGAGTATCGATGTTCACGGT+GGG | 0.631980 | 8:-56919390 | None:intergenic |
| CTCTTCGACAAGTATCACCC+TGG | 0.650081 | 8:+56919309 | MsG0880045286.01.T01:CDS |
| CCACTTTGCCGAAGTAACCA+GGG | 0.680564 | 8:-56919326 | None:intergenic |
| AAGAAGATTAAGGAAGCTGG+TGG | 0.688253 | 8:+56919582 | MsG0880045286.01.T01:CDS |
| ACTTGTCGAAGAGGATGCGG+TGG | 0.693301 | 8:-56919299 | None:intergenic |
| ACCTCAAGAAGTGAAGGACA+AGG | 0.699258 | 8:+56919425 | MsG0880045286.01.T01:CDS |
| GAGAGTATCGATGTTCACGG+TGG | 0.722248 | 8:-56919391 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ACAACAACACTCTGATTCTT+CGG | - | Chr8:56919531-56919550 | None:intergenic | 35.0% | |
| GATTGCTGAGAAGAAGATTA+AGG | + | Chr8:56919572-56919591 | MsG0880045286.01.T01:CDS | 35.0% | |
| TGATTGATGTTACGCAGTTT+GGG | + | Chr8:56919475-56919494 | MsG0880045286.01.T01:CDS | 35.0% | |
| !! | TTCTTTAAGGTTCTTGGGAA+AGG | + | Chr8:56919498-56919517 | MsG0880045286.01.T01:CDS | 35.0% |
| !! | TTGGGTTCTTTAAGGTTCTT+GGG | + | Chr8:56919493-56919512 | MsG0880045286.01.T01:CDS | 35.0% |
| !! | TTTGGGTTCTTTAAGGTTCT+TGG | + | Chr8:56919492-56919511 | MsG0880045286.01.T01:CDS | 35.0% |
| AACTGCGTAACATCAATCAC+AGG | - | Chr8:56919474-56919493 | None:intergenic | 40.0% | |
| AAGAAGATTAAGGAAGCTGG+TGG | + | Chr8:56919582-56919601 | MsG0880045286.01.T01:CDS | 40.0% | |
| AAGTATCACCCTGGTTACTT+CGG | + | Chr8:56919318-56919337 | MsG0880045286.01.T01:CDS | 40.0% | |
| GAGAAGAAGATTAAGGAAGC+TGG | + | Chr8:56919579-56919598 | MsG0880045286.01.T01:CDS | 40.0% | |
| GTGATTGATGTTACGCAGTT+TGG | + | Chr8:56919474-56919493 | MsG0880045286.01.T01:CDS | 40.0% | |
| TCTTCTACCTCAAGAAGTGA+AGG | + | Chr8:56919419-56919438 | MsG0880045286.01.T01:CDS | 40.0% | |
| ! | TACGCAGTTTGGGTTCTTTA+AGG | + | Chr8:56919485-56919504 | MsG0880045286.01.T01:CDS | 40.0% |
| ! | TGTTGTTGTTAAAGCGAAGC+TGG | + | Chr8:56919542-56919561 | MsG0880045286.01.T01:CDS | 40.0% |
| ACCTCAAGAAGTGAAGGACA+AGG | + | Chr8:56919425-56919444 | MsG0880045286.01.T01:CDS | 45.0% | |
| GATACTTGTCGAAGAGGATG+CGG | - | Chr8:56919305-56919324 | None:intergenic | 45.0% | |
| !! | AGAGTATCGATGTTCACGGT+GGG | - | Chr8:56919393-56919412 | None:intergenic | 45.0% |
| !! | CCAGAGAGTATCGATGTTCA+CGG | - | Chr8:56919397-56919416 | None:intergenic | 45.0% |
| !! | TGGGACAGTGGAATTTGTTG+CGG | - | Chr8:56919374-56919393 | None:intergenic | 45.0% |
| CAGGGTGATACTTGTCGAAG+AGG | - | Chr8:56919311-56919330 | None:intergenic | 50.0% | |
| CCACTTTGCCGAAGTAACCA+GGG | - | Chr8:56919329-56919348 | None:intergenic | 50.0% | |
| CCGTGAACATCGATACTCTC+TGG | + | Chr8:56919394-56919413 | MsG0880045286.01.T01:CDS | 50.0% | |
| CCTGGTTACTTCGGCAAAGT+GGG | + | Chr8:56919327-56919346 | MsG0880045286.01.T01:CDS | 50.0% | |
| CTCTTCGACAAGTATCACCC+TGG | + | Chr8:56919309-56919328 | MsG0880045286.01.T01:CDS | 50.0% | |
| GCCTTGTCCTTCACTTCTTG+AGG | - | Chr8:56919429-56919448 | None:intergenic | 50.0% | |
| ! | GGACAAGGCATCAAAGTCGA+AGG | + | Chr8:56919440-56919459 | MsG0880045286.01.T01:CDS | 50.0% |
| !! | GAGAGTATCGATGTTCACGG+TGG | - | Chr8:56919394-56919413 | None:intergenic | 50.0% |
| ACTTGTCGAAGAGGATGCGG+TGG | - | Chr8:56919302-56919321 | None:intergenic | 55.0% | |
| CCCACTTTGCCGAAGTAACC+AGG | - | Chr8:56919330-56919349 | None:intergenic | 55.0% | |
| CCCTGGTTACTTCGGCAAAG+TGG | + | Chr8:56919326-56919345 | MsG0880045286.01.T01:CDS | 55.0% | |
| CGATGTTCACGGTGGGACAG+TGG | - | Chr8:56919386-56919405 | None:intergenic | 60.0% | |
| TGTCGAAGAGGATGCGGTGG+TGG | - | Chr8:56919299-56919318 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr8 | gene | 56919291 | 56919626 | 56919291 | ID=MsG0880045286.01;Name=MsG0880045286.01 |
| Chr8 | mRNA | 56919291 | 56919626 | 56919291 | ID=MsG0880045286.01.T01;Parent=MsG0880045286.01;Name=MsG0880045286.01.T01;_AED=0.48;_eAED=0.48;_QI=0|-1|0|1|-1|1|1|0|111 |
| Chr8 | exon | 56919291 | 56919626 | 56919291 | ID=MsG0880045286.01.T01:exon:1204;Parent=MsG0880045286.01.T01 |
| Chr8 | CDS | 56919291 | 56919626 | 56919291 | ID=MsG0880045286.01.T01:cds;Parent=MsG0880045286.01.T01 |
| Gene Sequence |
| Protein sequence |
