AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480021293.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480021293.01.T01 MTR_4g071000 90.805 174 2 1 1 174 1 160 1.29e-110 312
MsG0480021293.01.T01 MTR_3g020660 81.977 172 17 1 1 172 1 158 1.41e-97 279
MsG0480021293.01.T01 MTR_3g020520 84.034 119 19 0 54 172 407 525 5.66e-66 210
MsG0480021293.01.T01 MTR_3g020640 72.593 135 30 1 38 172 1003 1130 2.75e-58 196
MsG0480021293.01.T01 MTR_3g020650 76.471 85 20 0 52 136 1028 1112 6.13e-37 135
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480021293.01.T01 AT1G17880 79.394 165 18 3 1 164 1 150 1.30e-87 254
MsG0480021293.01.T01 AT1G73230 78.947 171 21 2 1 170 1 157 9.70e-83 242

Find 70 sgRNAs with CRISPR-Local

Find 156 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGAGGAGGTTAATATCTTTA+AGG 0.165061 4:-56386507 MsG0480021293.01.T01:CDS
CTGCTTCAGGAGCCTGCTTC+TGG 0.192683 4:+56385803 None:intergenic
GAAGATTCGGTTTAAGTTTA+AGG 0.198576 4:+56388044 None:intergenic
AAGTTTAAGGTCAGTGATTT+AGG 0.220905 4:+56388057 None:intergenic
TAATTTCTTACTCTTTGTTT+GGG 0.224790 4:+56386290 None:intergenic
GTAATTTCTTACTCTTTGTT+TGG 0.245420 4:+56386289 None:intergenic
CATTCACACCTATTCTCTTC+AGG 0.292092 4:+56386543 None:intergenic
ATTGTTCAGCTATCTTCTTC+AGG 0.297553 4:+56385827 None:intergenic
TTTCACTTCTACAGAGTTGC+AGG 0.310509 4:-56385985 MsG0480021293.01.T01:intron
ACAGATTTAATTCAAGTATT+CGG 0.319393 4:+56385570 None:intergenic
GTTCAAAGCACATACCTAAT+TGG 0.327309 4:+56385940 None:intergenic
GCTGAACCAGCACCTGCTTC+AGG 0.334925 4:+56385790 None:intergenic
GTCTCTCCTGGGACAAGCTC+TGG 0.335105 4:+56385736 None:intergenic
CCTAGCATTATCCACCAATT+AGG 0.352096 4:-56385954 MsG0480021293.01.T01:intron
TACCTTTGGGGTTTAAGAAC+TGG 0.359158 4:+56386471 None:intergenic
AAACAAAAGGCAGAAAATTA+CGG 0.379527 4:+56386630 None:intergenic
AAGGTACCTTTGTGAAGATT+CGG 0.407308 4:+56388031 None:intergenic
CGTCCATTGCTGCTAATACA+TGG 0.419485 4:-56386329 MsG0480021293.01.T01:CDS
GCTGTCTCAAAAGTCTCTCC+TGG 0.443808 4:+56385724 None:intergenic
AACAAAAGGCAGAAAATTAC+GGG 0.449258 4:+56386631 None:intergenic
ATTCACACCTATTCTCTTCA+GGG 0.451011 4:+56386544 None:intergenic
TATTAACCTCCTCAATTTGT+GGG 0.452604 4:+56386516 None:intergenic
ATGGGCTGATATTACCTTTG+GGG 0.456873 4:+56386459 None:intergenic
CAGAAGCAGGCTCCTGAAGC+AGG 0.458056 4:-56385802 MsG0480021293.01.T01:CDS
GGTCAGTGATTTAGGACAAA+GGG 0.460327 4:+56388065 None:intergenic
TCCTCCGGTTCGAACAGAAC+CGG 0.461978 4:+56387052 None:intergenic
CAGAGCACCCTGAAGAGAAT+AGG 0.468639 4:-56386551 MsG0480021293.01.T01:CDS
ATAACAAATCTAACTATCCT+TGG 0.472981 4:+56385690 None:intergenic
TGTTTACCTCAGAAAGAAGA+AGG 0.477565 4:-56386612 MsG0480021293.01.T01:CDS
TCTGTTCGAACCGGAGGAAA+GGG 0.482236 4:-56387047 MsG0480021293.01.T01:CDS
AAACAGTTTGAATTAGATGA+AGG 0.483183 4:+56385544 None:intergenic
ATATTAACCTCCTCAATTTG+TGG 0.484190 4:+56386515 None:intergenic
TTTCATATTTAAGTATTTCA+GGG 0.489459 4:-56385626 MsG0480021293.01.T01:three_prime_UTR
CAGGCTCCTGAAGCAGGTGC+TGG 0.494992 4:-56385796 MsG0480021293.01.T01:CDS
TAATTCAAACTGTTTCTGCT+TGG 0.495425 4:-56385536 MsG0480021293.01.T01:three_prime_UTR
AGCTGAACAATTCCAGAAGC+AGG 0.497000 4:-56385815 MsG0480021293.01.T01:CDS
CCTAATTGGTGGATAATGCT+AGG 0.497579 4:+56385954 None:intergenic
CAACAACTACAGATGACAAA+AGG 0.502653 4:-56386577 MsG0480021293.01.T01:CDS
GATGTCCCAGAGCTTGTCCC+AGG 0.514432 4:-56385742 MsG0480021293.01.T01:CDS
AACCTCCTCAATTTGTGGGA+TGG 0.523460 4:+56386520 None:intergenic
CATCTGTAGTTGTTGTCTTG+TGG 0.525038 4:+56386585 None:intergenic
TTCTGTTCGAACCGGAGGAA+AGG 0.525397 4:-56387048 MsG0480021293.01.T01:CDS
GCTAATACATGGGTTGTTAG+TGG 0.526432 4:-56386318 MsG0480021293.01.T01:CDS
TCTCTCCTGGGACAAGCTCT+GGG 0.528395 4:+56385737 None:intergenic
GAATGCCATCCCACAAATTG+AGG 0.534495 4:-56386525 MsG0480021293.01.T01:CDS
AACCCATGTATTAGCAGCAA+TGG 0.539996 4:+56386326 None:intergenic
GACAGCTGCTGAGGAGGCCA+AGG 0.540301 4:-56385707 MsG0480021293.01.T01:CDS
TTCTTTCTGAGGTAAACAAA+AGG 0.543476 4:+56386617 None:intergenic
TCTGACGGTACCCTTTCCTC+CGG 0.545452 4:+56387037 None:intergenic
TCTTCTTCACACTGAGTCTG+AGG 0.552196 4:-56388093 MsG0480021293.01.T01:five_prime_UTR
AATTTCTTACTCTTTGTTTG+GGG 0.553423 4:+56386291 None:intergenic
GGACGGCCTTCTTCTTTCTG+AGG 0.559380 4:+56386606 None:intergenic
TGTAGTTGTTGTCTTGTGGA+CGG 0.561453 4:+56386589 None:intergenic
CTAATTGGTGGATAATGCTA+GGG 0.563275 4:+56385955 None:intergenic
ATGGCCGGTTCTGTTCGAAC+CGG 0.568678 4:-56387056 MsG0480021293.01.T01:CDS
GCCGGTTCTGTTCGAACCGG+AGG 0.569814 4:-56387053 MsG0480021293.01.T01:CDS
CTGTCTCAAAAGTCTCTCCT+GGG 0.571093 4:+56385725 None:intergenic
GTCCATTGCTGCTAATACAT+GGG 0.579320 4:-56386328 MsG0480021293.01.T01:CDS
ACTACACGAGAGAGAAGATA+AGG 0.583989 4:+56388121 None:intergenic
ATCCAGTTCTTAAACCCCAA+AGG 0.585033 4:-56386473 MsG0480021293.01.T01:intron
GAAAAGTTGATGAAGATGGC+CGG 0.594138 4:-56387071 MsG0480021293.01.T01:CDS
TCGTGAAAAGTTGATGAAGA+TGG 0.598987 4:-56387075 MsG0480021293.01.T01:CDS
AGGTCAGTGATTTAGGACAA+AGG 0.599373 4:+56388064 None:intergenic
CAAAGCACATACCTAATTGG+TGG 0.599520 4:+56385943 None:intergenic
GAGGAAAGGGTACCGTCAGA+AGG 0.619370 4:-56387034 MsG0480021293.01.T01:intron
AGACTCAGTGTGAAGAAGAA+CGG 0.635251 4:+56388097 None:intergenic
CATATAAATATACCTTCTGA+CGG 0.639530 4:+56387022 None:intergenic
TGCCATCCCACAAATTGAGG+AGG 0.649748 4:-56386522 MsG0480021293.01.T01:CDS
GTCAGTGATTTAGGACAAAG+GGG 0.667143 4:+56388066 None:intergenic
CTTAAACCGAATCTTCACAA+AGG 0.688327 4:-56388037 MsG0480021293.01.T01:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ACAATTAAAAAAAATTGATT+TGG + Chr4:56385803-56385822 None:intergenic 10.0%
!! TTTGAATATTATTAAAAAAC+AGG - Chr4:56387797-56387816 MsG0480021293.01.T01:intron 10.0%
!!! ATTTATTCTTTTTATATGAT+AGG - Chr4:56387241-56387260 MsG0480021293.01.T01:intron 10.0%
!!! CAATTAAAAAAAATTGATTT+GGG + Chr4:56385802-56385821 None:intergenic 10.0%
!!! TATTATCAATTTAGTTTTAT+AGG - Chr4:56385766-56385785 MsG0480021293.01.T01:CDS 10.0%
!! AAAAACAAGTTATATTAGAA+TGG + Chr4:56386685-56386704 None:intergenic 15.0%
!! ATAAAAGATGCAAAAATAAT+TGG + Chr4:56386506-56386525 None:intergenic 15.0%
!! TTTCATATTTAAGTATTTCA+GGG - Chr4:56388039-56388058 MsG0480021293.01.T01:five_prime_UTR 15.0%
!!! AATATTTTAGATATATCTGT+CGG - Chr4:56386749-56386768 MsG0480021293.01.T01:intron 15.0%
!!! TCTATTATGTGTTATTATAT+AGG - Chr4:56386074-56386093 MsG0480021293.01.T01:intron 15.0%
!!! TGAAATAATATGTCAATTTT+AGG + Chr4:56387665-56387684 None:intergenic 15.0%
!!! TTATTTTTGCATCTTTTATT+AGG - Chr4:56386507-56386526 MsG0480021293.01.T01:CDS 15.0%
!!! TTGTAATATTTTATGGTATA+TGG - Chr4:56386104-56386123 MsG0480021293.01.T01:intron 15.0%
!!! TTTTCATATTTAAGTATTTC+AGG - Chr4:56388038-56388057 MsG0480021293.01.T01:five_prime_UTR 15.0%
!! AAGAAACATTCTTTAAACAA+TGG + Chr4:56386949-56386968 None:intergenic 20.0%
!! AATAAATGCTGGAAATATTA+TGG + Chr4:56387228-56387247 None:intergenic 20.0%
!! AATACTTTAAATGAAAACTC+TGG - Chr4:56387473-56387492 MsG0480021293.01.T01:intron 20.0%
!! ACAGATTTAATTCAAGTATT+CGG + Chr4:56388098-56388117 None:intergenic 20.0%
!! ATAAATGCTGGAAATATTAT+GGG + Chr4:56387227-56387246 None:intergenic 20.0%
!! CATATAAAAAGAATAAATGC+TGG + Chr4:56387239-56387258 None:intergenic 20.0%
!! TTTATATGTGAAACTCATTA+TGG - Chr4:56386658-56386677 MsG0480021293.01.T01:intron 20.0%
!!! TAATTTCTTACTCTTTGTTT+GGG + Chr4:56387378-56387397 None:intergenic 20.0%
!!! TATATCATTTTGTTCATCAT+CGG + Chr4:56385914-56385933 None:intergenic 20.0%
!!! TTTTGAAAAAAAACAGAAGA+TGG + Chr4:56388005-56388024 None:intergenic 20.0%
! AAACAAAAGGCAGAAAATTA+CGG + Chr4:56387038-56387057 None:intergenic 25.0%
! AAACAGTTTGAATTAGATGA+AGG + Chr4:56388124-56388143 None:intergenic 25.0%
! AAATAGAGTTGAAGTAAAGA+AGG + Chr4:56385656-56385675 None:intergenic 25.0%
! AATATTCAAAACGTTCAACT+TGG + Chr4:56387787-56387806 None:intergenic 25.0%
! AGAAAACAATCAAAGCTTAA+TGG + Chr4:56387304-56387323 None:intergenic 25.0%
! ATAACAAATCTAACTATCCT+TGG + Chr4:56387978-56387997 None:intergenic 25.0%
! ATATTCAAAACGTTCAACTT+GGG + Chr4:56387786-56387805 None:intergenic 25.0%
! CAATGCTATACAACTAATAT+TGG + Chr4:56386717-56386736 None:intergenic 25.0%
! CATATAAATATACCTTCTGA+CGG + Chr4:56386646-56386665 None:intergenic 25.0%
! GCAATAGATATATGAAGAAA+AGG + Chr4:56387404-56387423 None:intergenic 25.0%
! GTAATTTCTTACTCTTTGTT+TGG + Chr4:56387379-56387398 None:intergenic 25.0%
!! AATTTCTTACTCTTTGTTTG+GGG + Chr4:56387377-56387396 None:intergenic 25.0%
!! ATTTTCCCACAATTTATGAA+CGG - Chr4:56387585-56387604 MsG0480021293.01.T01:intron 25.0%
!! GTGGTTTGATAATATGAATA+AGG - Chr4:56386416-56386435 MsG0480021293.01.T01:intron 25.0%
!!! AACTTTTTCAAAGGTTTGTT+TGG - Chr4:56387745-56387764 MsG0480021293.01.T01:intron 25.0%
!!! GTGAGTGTTTTTATAGTTAA+GGG - Chr4:56385863-56385882 MsG0480021293.01.T01:intron 25.0%
!!! TGAGTGTTTTTATAGTTAAG+GGG - Chr4:56385864-56385883 MsG0480021293.01.T01:intron 25.0%
!!! TGTGAGTGTTTTTATAGTTA+AGG - Chr4:56385862-56385881 MsG0480021293.01.T01:intron 25.0%
!!! TTATATTTTAGAGTACACCA+GGG - Chr4:56387559-56387578 MsG0480021293.01.T01:intron 25.0%
AAAAACAGGACCAGATAATT+TGG - Chr4:56387811-56387830 MsG0480021293.01.T01:intron 30.0%
AAAATGAACAGATTCAGCAT+AGG + Chr4:56387521-56387540 None:intergenic 30.0%
AACAAAAGGCAGAAAATTAC+GGG + Chr4:56387037-56387056 None:intergenic 30.0%
AGAGTTGTAGTCATTGAAAT+AGG - Chr4:56386222-56386241 MsG0480021293.01.T01:intron 30.0%
ATATTAACCTCCTCAATTTG+TGG + Chr4:56387153-56387172 None:intergenic 30.0%
TATTAACCTCCTCAATTTGT+GGG + Chr4:56387152-56387171 None:intergenic 30.0%
TGAGGAGGTTAATATCTTTA+AGG - Chr4:56387158-56387177 MsG0480021293.01.T01:intron 30.0%
TTTGATGCACATAAGGAAAT+GGG - Chr4:56386290-56386309 MsG0480021293.01.T01:intron 30.0%
! AAGTTTAAGGTCAGTGATTT+AGG + Chr4:56385611-56385630 None:intergenic 30.0%
! ATATCTGTCGGTTGTTATTA+GGG - Chr4:56386761-56386780 MsG0480021293.01.T01:intron 30.0%
! ATCATTTTCTTCTTGTACAG+TGG + Chr4:56387900-56387919 None:intergenic 30.0%
! GAAGATTCGGTTTAAGTTTA+AGG + Chr4:56385624-56385643 None:intergenic 30.0%
! TATATCTGTCGGTTGTTATT+AGG - Chr4:56386760-56386779 MsG0480021293.01.T01:intron 30.0%
! TCTGTTCATTTTGCAATATC+TGG - Chr4:56387529-56387548 MsG0480021293.01.T01:intron 30.0%
! TTTTCCCACAATTTATGAAC+GGG - Chr4:56387586-56387605 MsG0480021293.01.T01:intron 30.0%
! TTTTGATGCACATAAGGAAA+TGG - Chr4:56386289-56386308 MsG0480021293.01.T01:intron 30.0%
!! AGAAGGTACTGATTTAATTG+AGG - Chr4:56385834-56385853 MsG0480021293.01.T01:CDS 30.0%
!! GAAGGTACTGATTTAATTGA+GGG - Chr4:56385835-56385854 MsG0480021293.01.T01:CDS 30.0%
!! TAATTCAAACTGTTTCTGCT+TGG - Chr4:56388129-56388148 MsG0480021293.01.T01:five_prime_UTR 30.0%
!! TTCAGGTTTTCCAAATTATC+TGG + Chr4:56387824-56387843 None:intergenic 30.0%
!! TTCTTTCTGAGGTAAACAAA+AGG + Chr4:56387051-56387070 None:intergenic 30.0%
!! TTTATTAGGCGTTAGTTTGT+TGG - Chr4:56386521-56386540 MsG0480021293.01.T01:CDS 30.0%
!!! ATCATGTGCTCTTTGTTTTT+GGG - Chr4:56387619-56387638 MsG0480021293.01.T01:intron 30.0%
!!! CTGATTTTTTGATGCACATA+AGG - Chr4:56386283-56386302 MsG0480021293.01.T01:intron 30.0%
!!! GTTATATTTTAGAGTACACC+AGG - Chr4:56387558-56387577 MsG0480021293.01.T01:intron 30.0%
!!! TATCATGTGCTCTTTGTTTT+TGG - Chr4:56387618-56387637 MsG0480021293.01.T01:intron 30.0%
!!! TGTGCTTTGAACTTTTTCAA+AGG - Chr4:56387736-56387755 MsG0480021293.01.T01:intron 30.0%
AATTGTTTCATGAGCTCAGA+AGG - Chr4:56385817-56385836 MsG0480021293.01.T01:CDS 35.0%
ACACACTCGAATATGCTTAA+GGG + Chr4:56386855-56386874 None:intergenic 35.0%
ATTCACACCTATTCTCTTCA+GGG + Chr4:56387124-56387143 None:intergenic 35.0%
ATTGTTCAGCTATCTTCTTC+AGG + Chr4:56387841-56387860 None:intergenic 35.0%
CAACAACTACAGATGACAAA+AGG - Chr4:56387088-56387107 MsG0480021293.01.T01:intron 35.0%
CAAGAATTCATGTCAGCATA+TGG + Chr4:56386801-56386820 None:intergenic 35.0%
CACATGAACATGTCATGAAA+AGG + Chr4:56386399-56386418 None:intergenic 35.0%
CTTAAACCGAATCTTCACAA+AGG - Chr4:56385628-56385647 MsG0480021293.01.T01:three_prime_UTR 35.0%
GTTCAAAGCACATACCTAAT+TGG + Chr4:56387728-56387747 None:intergenic 35.0%
TCAGAGTAAAAGAAAGAACG+AGG + Chr4:56386898-56386917 None:intergenic 35.0%
TCTGTGCGGAAGAAAATTAT+TGG - Chr4:56385956-56385975 MsG0480021293.01.T01:CDS 35.0%
TGTGTGAGCTGAATTTAACT+TGG - Chr4:56386144-56386163 MsG0480021293.01.T01:intron 35.0%
TGTTTACCTCAGAAAGAAGA+AGG - Chr4:56387053-56387072 MsG0480021293.01.T01:CDS 35.0%
TTTCACGATTCATCTGAGAA+AGG + Chr4:56386578-56386597 None:intergenic 35.0%
! AAGGTACCTTTGTGAAGATT+CGG + Chr4:56385637-56385656 None:intergenic 35.0%
! AGGAATTGTGAAGTTTTGCT+TGG + Chr4:56386878-56386897 None:intergenic 35.0%
! CTAATTGGTGGATAATGCTA+GGG + Chr4:56387713-56387732 None:intergenic 35.0%
! CTTTTCATGACATGTTCATG+TGG - Chr4:56386397-56386416 MsG0480021293.01.T01:intron 35.0%
! GACTTTCTTCTCTGTAGTTT+TGG - Chr4:56386478-56386497 MsG0480021293.01.T01:CDS 35.0%
! TATGGGCTGATATTACCTTT+GGG + Chr4:56387210-56387229 None:intergenic 35.0%
! TTATGGGCTGATATTACCTT+TGG + Chr4:56387211-56387230 None:intergenic 35.0%
!! AGGGTTTTGTAGGTATAAGA+AGG - Chr4:56385519-56385538 MsG0480021293.01.T01:three_prime_UTR 35.0%
!! GGGTTTTGTAGGTATAAGAA+GGG - Chr4:56385520-56385539 MsG0480021293.01.T01:three_prime_UTR 35.0%
!! TCGTGAAAAGTTGATGAAGA+TGG - Chr4:56386590-56386609 MsG0480021293.01.T01:CDS 35.0%
!!! TCTTCTGAGATTTTTCTGTG+CGG - Chr4:56385942-56385961 MsG0480021293.01.T01:intron 35.0%
AACCCATGTATTAGCAGCAA+TGG + Chr4:56387342-56387361 None:intergenic 40.0%
AATTGTGGGAAAATATGCCC+TGG + Chr4:56387579-56387598 None:intergenic 40.0%
ACTACACGAGAGAGAAGATA+AGG + Chr4:56385547-56385566 None:intergenic 40.0%
AGACTCAGTGTGAAGAAGAA+CGG + Chr4:56385571-56385590 None:intergenic 40.0%
ATCCAGTTCTTAAACCCCAA+AGG - Chr4:56387192-56387211 MsG0480021293.01.T01:intron 40.0%
ATTATTGGAGACCTGAACTG+AGG - Chr4:56385971-56385990 MsG0480021293.01.T01:CDS 40.0%
CAAAGCACATACCTAATTGG+TGG + Chr4:56387725-56387744 None:intergenic 40.0%
CACACTCGAATATGCTTAAG+GGG + Chr4:56386854-56386873 None:intergenic 40.0%
CATCTGTAGTTGTTGTCTTG+TGG + Chr4:56387083-56387102 None:intergenic 40.0%
CATTCACACCTATTCTCTTC+AGG + Chr4:56387125-56387144 None:intergenic 40.0%
CCTAGCATTATCCACCAATT+AGG - Chr4:56387711-56387730 MsG0480021293.01.T01:intron 40.0%
CGAATATGCTTAAGGGGAAT+GGG + Chr4:56386848-56386867 None:intergenic 40.0%
CTAGCCCGTTCATAAATTGT+GGG + Chr4:56387593-56387612 None:intergenic 40.0%
GAATATGCTTAAGGGGAATG+GGG + Chr4:56386847-56386866 None:intergenic 40.0%
GACACACTCGAATATGCTTA+AGG + Chr4:56386856-56386875 None:intergenic 40.0%
GTCCATTGCTGCTAATACAT+GGG - Chr4:56387337-56387356 MsG0480021293.01.T01:intron 40.0%
TCAAAACGTTCAACTTGGGT+AGG + Chr4:56387782-56387801 None:intergenic 40.0%
TCGAATATGCTTAAGGGGAA+TGG + Chr4:56386849-56386868 None:intergenic 40.0%
TCTAGCCCGTTCATAAATTG+TGG + Chr4:56387594-56387613 None:intergenic 40.0%
TGGTTGCACTTGATTTCATC+AGG - Chr4:56388149-56388168 MsG0480021293.01.T01:five_prime_UTR 40.0%
TGTAGTTGTTGTCTTGTGGA+CGG + Chr4:56387079-56387098 None:intergenic 40.0%
TTTCACTTCTACAGAGTTGC+AGG - Chr4:56387680-56387699 MsG0480021293.01.T01:intron 40.0%
! AGGTCAGTGATTTAGGACAA+AGG + Chr4:56385604-56385623 None:intergenic 40.0%
! ATGGGCTGATATTACCTTTG+GGG + Chr4:56387209-56387228 None:intergenic 40.0%
! CCTAATTGGTGGATAATGCT+AGG + Chr4:56387714-56387733 None:intergenic 40.0%
! GCTAATACATGGGTTGTTAG+TGG - Chr4:56387347-56387366 MsG0480021293.01.T01:intron 40.0%
! GGTCAGTGATTTAGGACAAA+GGG + Chr4:56385603-56385622 None:intergenic 40.0%
! GTCAGTGATTTAGGACAAAG+GGG + Chr4:56385602-56385621 None:intergenic 40.0%
!! GAAAAGTTGATGAAGATGGC+CGG - Chr4:56386594-56386613 MsG0480021293.01.T01:CDS 40.0%
!! TACCTTTGGGGTTTAAGAAC+TGG + Chr4:56387197-56387216 None:intergenic 40.0%
AACCTCCTCAATTTGTGGGA+TGG + Chr4:56387148-56387167 None:intergenic 45.0%
AGCTGAACAATTCCAGAAGC+AGG - Chr4:56387850-56387869 MsG0480021293.01.T01:intron 45.0%
CGTCCATTGCTGCTAATACA+TGG - Chr4:56387336-56387355 MsG0480021293.01.T01:intron 45.0%
CTGTCTCAAAAGTCTCTCCT+GGG + Chr4:56387943-56387962 None:intergenic 45.0%
GAAGAAAGTCAAGACACAGC+AGG + Chr4:56386468-56386487 None:intergenic 45.0%
GAATGCCATCCCACAAATTG+AGG - Chr4:56387140-56387159 MsG0480021293.01.T01:intron 45.0%
TCTTCTTCACACTGAGTCTG+AGG - Chr4:56385572-56385591 MsG0480021293.01.T01:three_prime_UTR 45.0%
TGAAACAGTCTCCTCAGTTC+AGG + Chr4:56385985-56386004 None:intergenic 45.0%
TTACGGGACAACTCAACTCT+TGG + Chr4:56387021-56387040 None:intergenic 45.0%
!!! ATTTTTTTTGTAATATTTTA+TGG - Chr4:56386097-56386116 MsG0480021293.01.T01:intron 5.0%
GCTGTCTCAAAAGTCTCTCC+TGG + Chr4:56387944-56387963 None:intergenic 50.0%
TCTGTTCGAACCGGAGGAAA+GGG - Chr4:56386618-56386637 MsG0480021293.01.T01:CDS 50.0%
TGCCATCCCACAAATTGAGG+AGG - Chr4:56387143-56387162 MsG0480021293.01.T01:intron 50.0%
TTCTGTTCGAACCGGAGGAA+AGG - Chr4:56386617-56386636 MsG0480021293.01.T01:CDS 50.0%
! CAGAGCACCCTGAAGAGAAT+AGG - Chr4:56387114-56387133 MsG0480021293.01.T01:intron 50.0%
!!! GACTTTTGAGACAGCTGCTG+AGG - Chr4:56387949-56387968 MsG0480021293.01.T01:intron 50.0%
!!! TTTTGAGACAGCTGCTGAGG+AGG - Chr4:56387952-56387971 MsG0480021293.01.T01:intron 50.0%
GGACGGCCTTCTTCTTTCTG+AGG + Chr4:56387062-56387081 None:intergenic 55.0%
TCTCTCCTGGGACAAGCTCT+GGG + Chr4:56387931-56387950 None:intergenic 55.0%
TCTGACGGTACCCTTTCCTC+CGG + Chr4:56386631-56386650 None:intergenic 55.0%
! ATGGCCGGTTCTGTTCGAAC+CGG - Chr4:56386609-56386628 MsG0480021293.01.T01:CDS 55.0%
! TCCTCCGGTTCGAACAGAAC+CGG + Chr4:56386616-56386635 None:intergenic 55.0%
!! GAGGAAAGGGTACCGTCAGA+AGG - Chr4:56386631-56386650 MsG0480021293.01.T01:CDS 55.0%
CAGAAGCAGGCTCCTGAAGC+AGG - Chr4:56387863-56387882 MsG0480021293.01.T01:intron 60.0%
CTGCTTCAGGAGCCTGCTTC+TGG + Chr4:56387865-56387884 None:intergenic 60.0%
GATGTCCCAGAGCTTGTCCC+AGG - Chr4:56387923-56387942 MsG0480021293.01.T01:intron 60.0%
GCTGAACCAGCACCTGCTTC+AGG + Chr4:56387878-56387897 None:intergenic 60.0%
GTCTCTCCTGGGACAAGCTC+TGG + Chr4:56387932-56387951 None:intergenic 60.0%
CAGGCTCCTGAAGCAGGTGC+TGG - Chr4:56387869-56387888 MsG0480021293.01.T01:intron 65.0%
GACAGCTGCTGAGGAGGCCA+AGG - Chr4:56387958-56387977 MsG0480021293.01.T01:intron 65.0%
! GCCGGTTCTGTTCGAACCGG+AGG - Chr4:56386612-56386631 MsG0480021293.01.T01:CDS 65.0%
Chromosome Type Strat End Strand Name
Chr4 gene 56385519 56388168 56385519 ID=MsG0480021293.01;Name=MsG0480021293.01
Chr4 mRNA 56385519 56388168 56385519 ID=MsG0480021293.01.T01;Parent=MsG0480021293.01;Name=MsG0480021293.01.T01;_AED=0.50;_eAED=0.52;_QI=131|0.8|0.83|1|0.8|0.83|6|180|174
Chr4 exon 56385519 56385868 56385519 ID=MsG0480021293.01.T01:exon:19075;Parent=MsG0480021293.01.T01
Chr4 exon 56385955 56385993 56385955 ID=MsG0480021293.01.T01:exon:19074;Parent=MsG0480021293.01.T01
Chr4 exon 56386301 56386357 56386301 ID=MsG0480021293.01.T01:exon:19073;Parent=MsG0480021293.01.T01
Chr4 exon 56386474 56386664 56386474 ID=MsG0480021293.01.T01:exon:19072;Parent=MsG0480021293.01.T01
Chr4 exon 56387035 56387102 56387035 ID=MsG0480021293.01.T01:exon:19071;Parent=MsG0480021293.01.T01
Chr4 exon 56388038 56388168 56388038 ID=MsG0480021293.01.T01:exon:19070;Parent=MsG0480021293.01.T01
Chr4 five_prime_UTR 56388038 56388168 56388038 ID=MsG0480021293.01.T01:five_prime_utr;Parent=MsG0480021293.01.T01
Chr4 CDS 56387035 56387102 56387035 ID=MsG0480021293.01.T01:cds;Parent=MsG0480021293.01.T01
Chr4 CDS 56386474 56386664 56386474 ID=MsG0480021293.01.T01:cds;Parent=MsG0480021293.01.T01
Chr4 CDS 56386301 56386357 56386301 ID=MsG0480021293.01.T01:cds;Parent=MsG0480021293.01.T01
Chr4 CDS 56385955 56385993 56385955 ID=MsG0480021293.01.T01:cds;Parent=MsG0480021293.01.T01
Chr4 CDS 56385699 56385868 56385699 ID=MsG0480021293.01.T01:cds;Parent=MsG0480021293.01.T01
Chr4 three_prime_UTR 56385519 56385698 56385519 ID=MsG0480021293.01.T01:three_prime_utr;Parent=MsG0480021293.01.T01
Gene Sequence

>MsG0480021293.01.T01

ATGAATCGTGAAAAGTTGATGAAGATGGCCGGTTCTGTTCGAACCGGAGGAAAGGGTACCGTCAGAAGAGTTGAGTTGTCCCGTAATTTTCTGCCTTTTGTTTACCTCAGAAAGAAGAAGGCCGTCCACAAGACAACAACTACAGATGACAAAAGGCTTCAGAGCACCCTGAAGAGAATAGGTGTGAATGCCATCCCACAAATTGAGGAGGTTAATATCTTTAAGGATGATGTAGTTATCCAGTTCTTAAACCCCAAAGTTCAAGCGTCCATTGCTGCTAATACATGGGTTGTTAGTGGTGCTCCCCAAACAAAGAAGTTGCAGGATATACTCCCTAGCATTATCCACCAATTAGGACCAGATAATTTGGAAAACCTGAAGAAGATAGCTGAACAATTCCAGAAGCAGGCTCCTGAAGCAGGTGCTGGTTCAGCCACTGTACAAGAAGAAAATGATGATGATGTCCCAGAGCTTGTCCCAGGAGAGACTTTTGAGACAGCTGCTGAGGAGGCCAAGGATAGTTAG
Protein sequence

>MsG0480021293.01.T01

MNREKLMKMAGSVRTGGKGTVRRVELSRNFLPFVYLRKKKAVHKTTTTDDKRLQSTLKRIGVNAIPQIEEVNIFKDDVVIQFLNPKVQASIAANTWVVSGAPQTKKLQDILPSIIHQLGPDNLENLKKIAEQFQKQAPEAGAGSATVQEENDDDVPELVPGETFETAAEEAKDS*