AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0880045407.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880045407.01.T01 MTR_1g096750 31.338 284 139 6 7 287 8 238 1.14e-33 125
MsG0880045407.01.T01 MTR_1g096670 32.090 268 126 4 22 287 16 229 1.23e-28 112
MsG0880045407.01.T01 MTR_1g097945 43.878 98 43 1 190 287 1 86 1.32e-13 72.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 113 sgRNAs with CRISPR-Local

Find 212 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAATTTCTGTACTTTATTTA+TGG 0.108080 8:-58844689 MsG0880045407.01.T01:three_prime_UTR
TAGTAGCCTCGAGGCGTTTC+TGG 0.185657 8:+58847115 None:intergenic
CATAAATAAAGTACAGAAAT+TGG 0.188699 8:+58844690 None:intergenic
ATAATATTATGATATGAAAA+TGG 0.227696 8:+58844793 None:intergenic
TAACATGTATGTTCGAAGTT+TGG 0.287346 8:-58844640 MsG0880045407.01.T01:three_prime_UTR
AATCGATTGCTGGAGTTTCT+AGG 0.295720 8:-58847285 MsG0880045407.01.T01:CDS
TTCAATTCAATTCAATTCAA+TGG 0.306877 8:-58847520 MsG0880045407.01.T01:exon
CGCTGAGATCCAACAAATAA+AGG 0.320692 8:-58847016 MsG0880045407.01.T01:CDS
TGTTGCCTTCCTTTATTTGT+TGG 0.321857 8:+58847007 None:intergenic
CATATAAAATACTGACAAAA+TGG 0.325192 8:+58844736 None:intergenic
TCAAATCAGAAAACAGATTC+AGG 0.327765 8:+58847229 None:intergenic
ATGGTTAATGACATTAGGTT+AGG 0.328897 8:+58847661 None:intergenic
CATCATGATGTTTCTGAAAC+AGG 0.355422 8:+58845761 None:intergenic
ATAACAAGATAGACACATTA+AGG 0.360441 8:+58845579 None:intergenic
TACACGTTGGAGGAGTTAAA+TGG 0.361640 8:+58847642 None:intergenic
CGCGAGAGCGCAATAATTAC+TGG 0.364578 8:+58847590 None:intergenic
AGGCCATTGTGTTGTTGGTC+AGG 0.366476 8:+58846100 None:intergenic
TGCAAATCTTAATCGATTGC+TGG 0.372258 8:-58847295 MsG0880045407.01.T01:CDS
GTTAAATGGTTAATGACATT+AGG 0.375151 8:+58847656 None:intergenic
AATGCTGCCATATGTCTTGC+AGG 0.377606 8:-58845717 MsG0880045407.01.T01:intron
TGATGATCAGCTTGATGAAT+TGG 0.379101 8:-58846007 MsG0880045407.01.T01:CDS
CTTTGAGGCCATTGTGTTGT+TGG 0.384634 8:+58846095 None:intergenic
GTGTTCATCGACCACAGAAA+TGG 0.393587 8:+58847251 None:intergenic
ACTATAGCATCCACCTGTTT+CGG 0.402786 8:+58847414 None:intergenic
CAAAAGATTGGCGTGGCTTA+CGG 0.408058 8:+58846028 None:intergenic
CAAACTCTAGAGAAGATACT+TGG 0.409551 8:+58845845 None:intergenic
TCAATTCAATTCAATTCAAT+GGG 0.410322 8:-58847519 MsG0880045407.01.T01:exon
GCGTTTCTGGACAACTAAAT+CGG 0.418455 8:+58847128 None:intergenic
AGCGGAGATATGGTTAGGAT+TGG 0.418871 8:+58847561 None:intergenic
CCGTCAAATCCATCGATTAC+GGG 0.419275 8:+58847447 None:intergenic
ATTGGCGTGGCTTACGGTTG+TGG 0.422804 8:+58846034 None:intergenic
GATATTCAACAACATGCTTA+AGG 0.426688 8:-58845929 MsG0880045407.01.T01:CDS
TGGTTAATGACATTAGGTTA+GGG 0.427272 8:+58847662 None:intergenic
CCCGTCAAATCCATCGATTA+CGG 0.429826 8:+58847446 None:intergenic
GAGTCCGTTACCAGTACAAT+TGG 0.432677 8:-58844848 MsG0880045407.01.T01:CDS
TTCTGATTTCTTTCGAACAA+TGG 0.436021 8:+58845807 None:intergenic
CGATTACGGGTCGGAGTTGG+TGG 0.436161 8:+58847460 None:intergenic
TAATATTATGATATGAAAAT+GGG 0.440340 8:+58844794 None:intergenic
AAAACAAAGGAGCGGAGATA+TGG 0.442850 8:+58847551 None:intergenic
ATGGTGTGTATGCACTTTGA+TGG 0.444321 8:-58844670 MsG0880045407.01.T01:three_prime_UTR
ATAAATAAAGTACAGAAATT+GGG 0.448091 8:+58844691 None:intergenic
AATGTGTCTATCTTGTTATG+TGG 0.451012 8:-58845575 MsG0880045407.01.T01:CDS
CAAGCTGATCATCAAAAGAT+TGG 0.455046 8:+58846016 None:intergenic
AAGCTCAAAGTTTACTTTGA+CGG 0.462271 8:-58847201 MsG0880045407.01.T01:CDS
ATTTATGATAAAGATATTGC+TGG 0.463117 8:-58845541 MsG0880045407.01.T01:CDS
TGTTCATCGACCACAGAAAT+GGG 0.465814 8:+58847252 None:intergenic
AAAGGAGCGGAGATATGGTT+AGG 0.478938 8:+58847556 None:intergenic
TGGTTGCGGCTGCTAGATAA+TGG 0.479100 8:+58847610 None:intergenic
ACTCAAATAGCGAGGAAACT+TGG 0.482837 8:-58844938 MsG0880045407.01.T01:intron
TATAATTCAGTTTAGTTCGA+TGG 0.485132 8:+58847064 None:intergenic
CAAGATACCTGCAAGACATA+TGG 0.485566 8:+58845710 None:intergenic
GCGGAGATATGGTTAGGATT+GGG 0.490106 8:+58847562 None:intergenic
CCCGTAATCGATGGATTTGA+CGG 0.491309 8:-58847447 MsG0880045407.01.T01:CDS
TGATGTTTCTGAAACAGGTC+AGG 0.492055 8:+58845766 None:intergenic
TTCTCCATCTCCAATTGTAC+TGG 0.495562 8:+58844838 None:intergenic
GGTAACGGACTCATTGTAGA+AGG 0.501723 8:+58844859 None:intergenic
CAGTTTAGTTCGATGGTCCT+CGG 0.508588 8:+58847071 None:intergenic
CATCGATTACGGGTCGGAGT+TGG 0.511400 8:+58847457 None:intergenic
GTATGTAGATAACTCAAACT+TGG 0.512951 8:+58844570 None:intergenic
CGTTTCTGGACAACTAAATC+GGG 0.512963 8:+58847129 None:intergenic
AAGTTTACTTTGACGGAGCA+TGG 0.514430 8:-58847194 MsG0880045407.01.T01:CDS
GGCCATTGTGTTGTTGGTCA+GGG 0.517088 8:+58846101 None:intergenic
TGAGGTTGGCGCTAGAATGT+TGG 0.518995 8:-58844888 MsG0880045407.01.T01:CDS
TACAGAGGCCAAGCTTATGC+AGG 0.522332 8:-58845905 MsG0880045407.01.T01:CDS
ACATATGGCAGCATTGCTGC+AGG 0.525649 8:+58845725 None:intergenic
GATTTGCATAAGCTGAGAGA+AGG 0.531916 8:+58847310 None:intergenic
CCGTAATCGATGGATTTGAC+GGG 0.535088 8:-58847446 MsG0880045407.01.T01:CDS
AAAAGTGAACGCATTGAGGT+TGG 0.536080 8:-58844902 MsG0880045407.01.T01:CDS
ATTCAATTCAATTCAATGGG+TGG 0.537321 8:-58847516 MsG0880045407.01.T01:exon
CGAGGCTACTAAGAATCACC+TGG 0.537322 8:-58847103 MsG0880045407.01.T01:CDS
GATTTCTTTCGAACAATGGA+GGG 0.537545 8:+58845811 None:intergenic
AGCGCAATAATTACTGGTTG+CGG 0.540851 8:+58847596 None:intergenic
CACAGAACAATGCGACTTTG+AGG 0.542941 8:+58846080 None:intergenic
TGATTTCTTTCGAACAATGG+AGG 0.549021 8:+58845810 None:intergenic
ATTTGCATAAGCTGAGAGAA+GGG 0.553924 8:+58847311 None:intergenic
ATCTCCAATTGTACTGGTAA+CGG 0.556871 8:+58844844 None:intergenic
GAGTGGCTTAGTGTCGTCAT+TGG 0.557628 8:+58846056 None:intergenic
TTGAATTGAATTCAAAACAA+AGG 0.563875 8:+58847538 None:intergenic
TGTGCGAACCTGCATAAGCT+TGG 0.565158 8:+58845897 None:intergenic
GGTCCTCGGATGTGTACTCC+AGG 0.565951 8:+58847085 None:intergenic
AAAGATATTGCTGGCACCTG+CGG 0.569472 8:-58845532 MsG0880045407.01.T01:intron
CTTCTTGCTCTCGTGAGAGA+AGG 0.572465 8:+58847155 None:intergenic
CAAATAGCGAGGAAACTTGG+AGG 0.580676 8:-58844935 MsG0880045407.01.T01:intron
CAACTCCGACCCGTAATCGA+TGG 0.585048 8:-58847456 MsG0880045407.01.T01:CDS
TATCATAATATTATGGCAAG+TGG 0.585551 8:-58844784 MsG0880045407.01.T01:exon
GAAATCAGAAGAAAGTTGCA+AGG 0.586986 8:-58845794 MsG0880045407.01.T01:CDS
AAATAGCGAGGAAACTTGGA+GGG 0.593670 8:-58844934 MsG0880045407.01.T01:intron
AGAGTTGACGACTGTTCTGA+AGG 0.603709 8:-58845875 MsG0880045407.01.T01:CDS
GTTACCAGTACAATTGGAGA+TGG 0.605407 8:-58844842 MsG0880045407.01.T01:CDS
GAGATCCAACAAATAAAGGA+AGG 0.606146 8:-58847012 MsG0880045407.01.T01:CDS
GAGATATGGTTAGGATTGGG+TGG 0.621294 8:+58847565 None:intergenic
AAACTCTAGAGAAGATACTT+GGG 0.625633 8:+58845846 None:intergenic
GTGACGACGATACACGTTGG+AGG 0.626080 8:+58847632 None:intergenic
CGTGGCTTACGGTTGTGGAG+TGG 0.628056 8:+58846039 None:intergenic
TTGCATAAGCTGAGAGAAGG+GGG 0.634466 8:+58847313 None:intergenic
CACCCTGACCAACAACACAA+TGG 0.634616 8:-58846103 MsG0880045407.01.T01:CDS
TTTGCATAAGCTGAGAGAAG+GGG 0.641548 8:+58847312 None:intergenic
CGTAATCGATGGATTTGACG+GGG 0.649344 8:-58847445 MsG0880045407.01.T01:CDS
TTGAATTCAAAACAAAGGAG+CGG 0.649387 8:+58847543 None:intergenic
TATAGTCAAGTTTATCGCCA+CGG 0.649797 8:-58847397 MsG0880045407.01.T01:CDS
TCAGTGTACTCCCATTTCTG+TGG 0.652198 8:-58847262 MsG0880045407.01.T01:CDS
ACGCCTATCGTCATAGTCCG+TGG 0.662689 8:+58847380 None:intergenic
CAAATCCATCGATTACGGGT+CGG 0.665025 8:+58847451 None:intergenic
TCGCCACGGACTATGACGAT+AGG 0.666817 8:-58847383 MsG0880045407.01.T01:CDS
CAACATGCTTAAGGATACAG+AGG 0.668373 8:-58845920 MsG0880045407.01.T01:CDS
TGATCATCAAAAGATTGGCG+TGG 0.674556 8:+58846021 None:intergenic
AATAGCGAGGAAACTTGGAG+GGG 0.679610 8:-58844933 MsG0880045407.01.T01:intron
ATGGTGACGACGATACACGT+TGG 0.708448 8:+58847629 None:intergenic
TCACCTGGAGTACACATCCG+AGG 0.710730 8:-58847088 MsG0880045407.01.T01:CDS
GGTGATTCTTAGTAGCCTCG+AGG 0.716187 8:+58847106 None:intergenic
ATATTATGGCAAGTGGTGTG+TGG 0.720183 8:-58844777 MsG0880045407.01.T01:three_prime_UTR
AGTTGTCCAGAAACGCCTCG+AGG 0.733118 8:-58847121 MsG0880045407.01.T01:CDS
AATATCACGTCAATATCACG+CGG 0.748291 8:+58847348 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATATTATAATTAAATAAA+GGG + Chr8:58845912-58845931 None:intergenic 0.0%
!! TATATATTATAATTAAATAA+AGG + Chr8:58845913-58845932 None:intergenic 0.0%
!! ATAATATTATGATATGAAAA+TGG + Chr8:58847493-58847512 None:intergenic 10.0%
!! TAATATTATGATATGAAAAT+GGG + Chr8:58847492-58847511 None:intergenic 10.0%
!! TAGAAAATCAAAAATATATA+CGG + Chr8:58847314-58847333 None:intergenic 10.0%
!!! ATTTTCATATCATAATATTA+TGG - Chr8:58847492-58847511 MsG0880045407.01.T01:CDS 10.0%
!! ATAAATAAAGTACAGAAATT+GGG + Chr8:58847595-58847614 None:intergenic 15.0%
!! CTATTTACAACTAATTAAAA+AGG + Chr8:58846799-58846818 None:intergenic 15.0%
!! TATTTAGTGTTATATTGTAT+TGG - Chr8:58845543-58845562 MsG0880045407.01.T01:CDS 15.0%
!!! AAGAAAAGTGTATAAATAAA+GGG - Chr8:58845479-58845498 MsG0880045407.01.T01:intron 15.0%
!!! ATTAAACCGAAAATTTTATA+GGG - Chr8:58845838-58845857 MsG0880045407.01.T01:CDS 15.0%
!!! CATATGTTGATTATTTTATT+AGG + Chr8:58846600-58846619 None:intergenic 15.0%
!!! CATTATAATTTTTATGACAA+TGG - Chr8:58846982-58847001 MsG0880045407.01.T01:intron 15.0%
!!! TATTAAACCGAAAATTTTAT+AGG - Chr8:58845837-58845856 MsG0880045407.01.T01:CDS 15.0%
!! AAAGTGGAATATATTGTATT+GGG - Chr8:58845503-58845522 MsG0880045407.01.T01:intron 20.0%
!! AAATCAAAAATATATACGGA+AGG + Chr8:58847310-58847329 None:intergenic 20.0%
!! ATTTATGATAAAGATATTGC+TGG - Chr8:58846742-58846761 MsG0880045407.01.T01:intron 20.0%
!! CATAAATAAAGTACAGAAAT+TGG + Chr8:58847596-58847615 None:intergenic 20.0%
!! CATATAAAATACTGACAAAA+TGG + Chr8:58847550-58847569 None:intergenic 20.0%
!! TCAATTCAATTCAATTCAAT+GGG - Chr8:58844759-58844778 MsG0880045407.01.T01:three_prime_UTR 20.0%
!! TCATTATTAGAAATAAACGT+TGG + Chr8:58845348-58845367 None:intergenic 20.0%
!! TGAGTATTTATTAAGTACTA+AGG - Chr8:58845742-58845761 MsG0880045407.01.T01:CDS 20.0%
!! TTCAATTCAATTCAATTCAA+TGG - Chr8:58844758-58844777 MsG0880045407.01.T01:three_prime_UTR 20.0%
!! TTGAATTGAATTCAAAACAA+AGG + Chr8:58844748-58844767 None:intergenic 20.0%
!!! AGAAAAGTGTATAAATAAAG+GGG - Chr8:58845480-58845499 MsG0880045407.01.T01:intron 20.0%
!!! ATGATACAACTAGTTTTTAT+AGG + Chr8:58847148-58847167 None:intergenic 20.0%
!!! CAAAAGTACAAGATAATAAT+AGG + Chr8:58847255-58847274 None:intergenic 20.0%
!!! CAAGAAAAGTGTATAAATAA+AGG - Chr8:58845478-58845497 MsG0880045407.01.T01:intron 20.0%
!!! CAATTTCTGTACTTTATTTA+TGG - Chr8:58847594-58847613 MsG0880045407.01.T01:five_prime_UTR 20.0%
!!! GACACTTTTTTTTAGAAAAT+GGG - Chr8:58845613-58845632 MsG0880045407.01.T01:intron 20.0%
!!! TAAAAAGTTGAACCTTTTTT+GGG + Chr8:58845304-58845323 None:intergenic 20.0%
!!! TTAAACCGAAAATTTTATAG+GGG - Chr8:58845839-58845858 MsG0880045407.01.T01:CDS 20.0%
! ACCACTTTGTAATATTGTTT+AGG - Chr8:58845702-58845721 MsG0880045407.01.T01:intron 25.0%
! ACCTAAACAATATTACAAAG+TGG + Chr8:58845706-58845725 None:intergenic 25.0%
! ACTAAGGTTAAACTAGTTAT+AGG - Chr8:58845758-58845777 MsG0880045407.01.T01:CDS 25.0%
! ATAACAAGATAGACACATTA+AGG + Chr8:58846707-58846726 None:intergenic 25.0%
! ATATTTGGACATTTATTCTG+AGG - Chr8:58846132-58846151 MsG0880045407.01.T01:intron 25.0%
! GAAAGTGGAATATATTGTAT+TGG - Chr8:58845502-58845521 MsG0880045407.01.T01:intron 25.0%
! GTTAAATGGTTAATGACATT+AGG + Chr8:58844630-58844649 None:intergenic 25.0%
! GTTGTCAAAATCATTATGTA+TGG - Chr8:58847084-58847103 MsG0880045407.01.T01:CDS 25.0%
! TATCATAATATTATGGCAAG+TGG - Chr8:58847499-58847518 MsG0880045407.01.T01:CDS 25.0%
! TCACAACATTTGCAAATAAT+TGG - Chr8:58847114-58847133 MsG0880045407.01.T01:CDS 25.0%
! TCCAAATATAAGAGTTAATG+AGG + Chr8:58846121-58846140 None:intergenic 25.0%
! TCCTCATTAACTCTTATATT+TGG - Chr8:58846117-58846136 MsG0880045407.01.T01:CDS 25.0%
!! TATAATTCAGTTTAGTTCGA+TGG + Chr8:58845222-58845241 None:intergenic 25.0%
!! TTGAAATGAGTCAGTTATTT+TGG - Chr8:58845590-58845609 MsG0880045407.01.T01:CDS 25.0%
!!! AATCTGTTTTCTGATTTGAT+TGG - Chr8:58845058-58845077 MsG0880045407.01.T01:intron 25.0%
!!! GGACACTTTTTTTTAGAAAA+TGG - Chr8:58845612-58845631 MsG0880045407.01.T01:intron 25.0%
!!! GTAAAAAGTTGAACCTTTTT+TGG + Chr8:58845305-58845324 None:intergenic 25.0%
!!! TGAAATGAGTCAGTTATTTT+GGG - Chr8:58845591-58845610 MsG0880045407.01.T01:CDS 25.0%
AACATTTGCAAATAATTGGC+AGG - Chr8:58847118-58847137 MsG0880045407.01.T01:CDS 30.0%
AAGCTCAAAGTTTACTTTGA+CGG - Chr8:58845082-58845101 MsG0880045407.01.T01:intron 30.0%
ACCCATTTAATCACTCTAAA+TGG - Chr8:58845788-58845807 MsG0880045407.01.T01:CDS 30.0%
AGAATACTTAAAATACAGCC+CGG + Chr8:58846676-58846695 None:intergenic 30.0%
ATATTGTTTAGGTGACTAGT+TGG - Chr8:58845713-58845732 MsG0880045407.01.T01:intron 30.0%
ATGGTTAATGACATTAGGTT+AGG + Chr8:58844625-58844644 None:intergenic 30.0%
ATTCAATTCAATTCAATGGG+TGG - Chr8:58844767-58844786 MsG0880045407.01.T01:three_prime_UTR 30.0%
ATTGTATTCTTGGTTATGCT+GGG - Chr8:58847044-58847063 MsG0880045407.01.T01:CDS 30.0%
CCATTTAATCACTCTAAATG+GGG - Chr8:58845790-58845809 MsG0880045407.01.T01:CDS 30.0%
CCCATTTAATCACTCTAAAT+GGG - Chr8:58845789-58845808 MsG0880045407.01.T01:CDS 30.0%
CGGAAATGACATATATTACA+GGG - Chr8:58845869-58845888 MsG0880045407.01.T01:CDS 30.0%
GACATCAACAATCATCATAA+AGG + Chr8:58847282-58847301 None:intergenic 30.0%
GATATTCAACAACATGCTTA+AGG - Chr8:58846354-58846373 MsG0880045407.01.T01:intron 30.0%
GGAAATGACATATATTACAG+GGG - Chr8:58845870-58845889 MsG0880045407.01.T01:CDS 30.0%
GTTTCATGAGCATTTCATAA+TGG - Chr8:58847015-58847034 MsG0880045407.01.T01:CDS 30.0%
TAACATGTATGTTCGAAGTT+TGG - Chr8:58847643-58847662 MsG0880045407.01.T01:five_prime_UTR 30.0%
TATTGTATTCTTGGTTATGC+TGG - Chr8:58847043-58847062 MsG0880045407.01.T01:CDS 30.0%
TCAAATCAGAAAACAGATTC+AGG + Chr8:58845057-58845076 None:intergenic 30.0%
TGGTTAATGACATTAGGTTA+GGG + Chr8:58844624-58844643 None:intergenic 30.0%
TGTATAAATAAAGGGGAAAG+TGG - Chr8:58845487-58845506 MsG0880045407.01.T01:intron 30.0%
TTCTGATTTCTTTCGAACAA+TGG + Chr8:58846479-58846498 None:intergenic 30.0%
TTGAATTCAAAACAAAGGAG+CGG + Chr8:58844743-58844762 None:intergenic 30.0%
! AAACTCTAGAGAAGATACTT+GGG + Chr8:58846440-58846459 None:intergenic 30.0%
! AATGTGTCTATCTTGTTATG+TGG - Chr8:58846708-58846727 MsG0880045407.01.T01:intron 30.0%
! CCCATTTAGAGTGATTAAAT+GGG + Chr8:58845792-58845811 None:intergenic 30.0%
! CCTGTAATATATGTCATTTC+CGG + Chr8:58845871-58845890 None:intergenic 30.0%
! GTACAATTATGTTTTGTCTC+CGG - Chr8:58846654-58846673 MsG0880045407.01.T01:intron 30.0%
! TACAAACCAAGTTTGAGTTA+TGG - Chr8:58847707-58847726 MsG0880045407.01.T01:five_prime_UTR 30.0%
! TACAATTATGTTTTGTCTCC+GGG - Chr8:58846655-58846674 MsG0880045407.01.T01:intron 30.0%
! TTTCGCCCCTATAAAATTTT+CGG + Chr8:58845847-58845866 None:intergenic 30.0%
! TTTTTGTTACTCAAATAGCG+AGG - Chr8:58847337-58847356 MsG0880045407.01.T01:CDS 30.0%
AATAGTATGTCACGATACCA+TGG - Chr8:58847177-58847196 MsG0880045407.01.T01:CDS 35.0%
AATATCACGTCAATATCACG+CGG + Chr8:58844938-58844957 None:intergenic 35.0%
AATTGATGAGCTTGAGACTT+AGG - Chr8:58846939-58846958 MsG0880045407.01.T01:intron 35.0%
ATTTGCATAAGCTGAGAGAA+GGG + Chr8:58844975-58844994 None:intergenic 35.0%
CAAAGTAGGTCAATTATACC+GGG + Chr8:58845443-58845462 None:intergenic 35.0%
CAAGCTGATCATCAAAAGAT+TGG + Chr8:58846270-58846289 None:intergenic 35.0%
CCAAAGTAGGTCAATTATAC+CGG + Chr8:58845444-58845463 None:intergenic 35.0%
CCGGAAATGACATATATTAC+AGG - Chr8:58845868-58845887 MsG0880045407.01.T01:CDS 35.0%
GAAATCAGAAGAAAGTTGCA+AGG - Chr8:58846489-58846508 MsG0880045407.01.T01:intron 35.0%
GAGATCCAACAAATAAAGGA+AGG - Chr8:58845271-58845290 MsG0880045407.01.T01:intron 35.0%
GATTTCTTTCGAACAATGGA+GGG + Chr8:58846475-58846494 None:intergenic 35.0%
GTATGTGTGTATGTGTGTAT+GGG - Chr8:58845942-58845961 MsG0880045407.01.T01:CDS 35.0%
TATAGTCAAGTTTATCGCCA+CGG - Chr8:58844886-58844905 MsG0880045407.01.T01:CDS 35.0%
TCATGAGCATTTCATAATGG+TGG - Chr8:58847018-58847037 MsG0880045407.01.T01:CDS 35.0%
TCTGTGTGAAAAACCAAAGT+AGG + Chr8:58845457-58845476 None:intergenic 35.0%
TGATGATCAGCTTGATGAAT+TGG - Chr8:58846276-58846295 MsG0880045407.01.T01:intron 35.0%
TGATTTCTTTCGAACAATGG+AGG + Chr8:58846476-58846495 None:intergenic 35.0%
TGCAAATCTTAATCGATTGC+TGG - Chr8:58844988-58845007 MsG0880045407.01.T01:intron 35.0%
TGTATGTGTGTATGTGTGTA+TGG - Chr8:58845941-58845960 MsG0880045407.01.T01:CDS 35.0%
! AAAATTAATGCGAGAGTGTG+AGG + Chr8:58846855-58846874 None:intergenic 35.0%
! AATTTTATAGGGGCGAAAAC+CGG - Chr8:58845849-58845868 MsG0880045407.01.T01:CDS 35.0%
! AGAGAGAGTTTTCGAAACTT+GGG - Chr8:58846319-58846338 MsG0880045407.01.T01:intron 35.0%
! ATCTCCAATTGTACTGGTAA+CGG + Chr8:58847442-58847461 None:intergenic 35.0%
! ATGGTGGTGTATTGTATTCT+TGG - Chr8:58847034-58847053 MsG0880045407.01.T01:CDS 35.0%
! CAAACTCTAGAGAAGATACT+TGG + Chr8:58846441-58846460 None:intergenic 35.0%
! CCCCATTTAGAGTGATTAAA+TGG + Chr8:58845793-58845812 None:intergenic 35.0%
! GGATTTTCTTGGCATTTTGT+AGG - Chr8:58847664-58847683 MsG0880045407.01.T01:five_prime_UTR 35.0%
! GTTCGAAGTTTGGATTTTCT+TGG - Chr8:58847653-58847672 MsG0880045407.01.T01:five_prime_UTR 35.0%
! TGTTGCCTTCCTTTATTTGT+TGG + Chr8:58845279-58845298 None:intergenic 35.0%
!! CATCATGATGTTTCTGAAAC+AGG + Chr8:58846525-58846544 None:intergenic 35.0%
!!! AATGCGTTCACTTTTTGGTA+AGG + Chr8:58847376-58847395 None:intergenic 35.0%
AAAACAAAGGAGCGGAGATA+TGG + Chr8:58844735-58844754 None:intergenic 40.0%
AAAAGTGAACGCATTGAGGT+TGG - Chr8:58847381-58847400 MsG0880045407.01.T01:CDS 40.0%
AAATAGCGAGGAAACTTGGA+GGG - Chr8:58847349-58847368 MsG0880045407.01.T01:CDS 40.0%
AAGTTTACTTTGACGGAGCA+TGG - Chr8:58845089-58845108 MsG0880045407.01.T01:intron 40.0%
AATCGATTGCTGGAGTTTCT+AGG - Chr8:58844998-58845017 MsG0880045407.01.T01:intron 40.0%
ACCAAAAAGTGAACGCATTG+AGG - Chr8:58847377-58847396 MsG0880045407.01.T01:CDS 40.0%
ACTATAGCATCCACCTGTTT+CGG + Chr8:58844872-58844891 None:intergenic 40.0%
ACTCAAATAGCGAGGAAACT+TGG - Chr8:58847345-58847364 MsG0880045407.01.T01:CDS 40.0%
AGTGTCAACAAACATCTCCA+TGG + Chr8:58847197-58847216 None:intergenic 40.0%
ATATTATGGCAAGTGGTGTG+TGG - Chr8:58847506-58847525 MsG0880045407.01.T01:exon 40.0%
ATGGTGTGTATGCACTTTGA+TGG - Chr8:58847613-58847632 MsG0880045407.01.T01:five_prime_UTR 40.0%
ATTAATTACTCCCTCCGTCT+CGG + Chr8:58845648-58845667 None:intergenic 40.0%
CAACATGCTTAAGGATACAG+AGG - Chr8:58846363-58846382 MsG0880045407.01.T01:intron 40.0%
CAAGATACCTGCAAGACATA+TGG + Chr8:58846576-58846595 None:intergenic 40.0%
CCGGTATAATTGACCTACTT+TGG - Chr8:58845441-58845460 MsG0880045407.01.T01:intron 40.0%
CGCTGAGATCCAACAAATAA+AGG - Chr8:58845267-58845286 MsG0880045407.01.T01:intron 40.0%
GAAGGCAACAAACCCAAAAA+AGG - Chr8:58845289-58845308 MsG0880045407.01.T01:intron 40.0%
GATTTGCATAAGCTGAGAGA+AGG + Chr8:58844976-58844995 None:intergenic 40.0%
GTATGGGTGTATGTGTGTAT+GGG - Chr8:58845958-58845977 MsG0880045407.01.T01:CDS 40.0%
GTTACCAGTACAATTGGAGA+TGG - Chr8:58847441-58847460 MsG0880045407.01.T01:CDS 40.0%
TACACGTTGGAGGAGTTAAA+TGG + Chr8:58844644-58844663 None:intergenic 40.0%
TGATCATCAAAAGATTGGCG+TGG + Chr8:58846265-58846284 None:intergenic 40.0%
TGTATGGGTGTATGTGTGTA+TGG - Chr8:58845957-58845976 MsG0880045407.01.T01:CDS 40.0%
TGTTCATCGACCACAGAAAT+GGG + Chr8:58845034-58845053 None:intergenic 40.0%
TTCTCCATCTCCAATTGTAC+TGG + Chr8:58847448-58847467 None:intergenic 40.0%
TTTGCATAAGCTGAGAGAAG+GGG + Chr8:58844974-58844993 None:intergenic 40.0%
! AGCGCAATAATTACTGGTTG+CGG + Chr8:58844690-58844709 None:intergenic 40.0%
! ATGGAGATGTTTGTTGACAC+TGG - Chr8:58847196-58847215 MsG0880045407.01.T01:CDS 40.0%
! CGTTTCTGGACAACTAAATC+GGG + Chr8:58845157-58845176 None:intergenic 40.0%
! GAGAGAGAGTTTTCGAAACT+TGG - Chr8:58846318-58846337 MsG0880045407.01.T01:intron 40.0%
! GCGTTTCTGGACAACTAAAT+CGG + Chr8:58845158-58845177 None:intergenic 40.0%
!! ACCTCAATGCGTTCACTTTT+TGG + Chr8:58847381-58847400 None:intergenic 40.0%
!! TGATGTTTCTGAAACAGGTC+AGG + Chr8:58846520-58846539 None:intergenic 40.0%
AAAGATATTGCTGGCACCTG+CGG - Chr8:58846751-58846770 MsG0880045407.01.T01:intron 45.0%
AAAGGAGCGGAGATATGGTT+AGG + Chr8:58844730-58844749 None:intergenic 45.0%
AATAGCGAGGAAACTTGGAG+GGG - Chr8:58847350-58847369 MsG0880045407.01.T01:CDS 45.0%
AATGCTGCCATATGTCTTGC+AGG - Chr8:58846566-58846585 MsG0880045407.01.T01:intron 45.0%
AGAGTTGACGACTGTTCTGA+AGG - Chr8:58846408-58846427 MsG0880045407.01.T01:intron 45.0%
AGCGGAGATATGGTTAGGAT+TGG + Chr8:58844725-58844744 None:intergenic 45.0%
AGCTTGAGACTTAGGATTGC+AGG - Chr8:58846947-58846966 MsG0880045407.01.T01:intron 45.0%
ATCCATGCAATATAGACCGC+AGG + Chr8:58846770-58846789 None:intergenic 45.0%
ATGAAGCATAGACTCCGACA+CGG + Chr8:58846064-58846083 None:intergenic 45.0%
ATGGGTCAGTTATTCCGAGA+CGG - Chr8:58845631-58845650 MsG0880045407.01.T01:intron 45.0%
CAAAAGATTGGCGTGGCTTA+CGG + Chr8:58846258-58846277 None:intergenic 45.0%
CAAATAGCGAGGAAACTTGG+AGG - Chr8:58847348-58847367 MsG0880045407.01.T01:CDS 45.0%
CACAGAACAATGCGACTTTG+AGG + Chr8:58846206-58846225 None:intergenic 45.0%
GAGATATGGTTAGGATTGGG+TGG + Chr8:58844721-58844740 None:intergenic 45.0%
GAGTCCGTTACCAGTACAAT+TGG - Chr8:58847435-58847454 MsG0880045407.01.T01:CDS 45.0%
GCGGAGATATGGTTAGGATT+GGG + Chr8:58844724-58844743 None:intergenic 45.0%
GTAGGTCAATTATACCGGGA+CGG + Chr8:58845439-58845458 None:intergenic 45.0%
GTGTTCATCGACCACAGAAA+TGG + Chr8:58845035-58845054 None:intergenic 45.0%
GTTGCTAACGTGCTATCACA+TGG - Chr8:58846818-58846837 MsG0880045407.01.T01:intron 45.0%
TCAGTGTACTCCCATTTCTG+TGG - Chr8:58845021-58845040 MsG0880045407.01.T01:intron 45.0%
TTGCATAAGCTGAGAGAAGG+GGG + Chr8:58844973-58844992 None:intergenic 45.0%
! CAAATCCATCGATTACGGGT+CGG + Chr8:58844835-58844854 None:intergenic 45.0%
! CAGTTTAGTTCGATGGTCCT+CGG + Chr8:58845215-58845234 None:intergenic 45.0%
! CCCGTAATCGATGGATTTGA+CGG - Chr8:58844836-58844855 MsG0880045407.01.T01:CDS 45.0%
! CCCGTCAAATCCATCGATTA+CGG + Chr8:58844840-58844859 None:intergenic 45.0%
! CCGTAATCGATGGATTTGAC+GGG - Chr8:58844837-58844856 MsG0880045407.01.T01:CDS 45.0%
! CCGTCAAATCCATCGATTAC+GGG + Chr8:58844839-58844858 None:intergenic 45.0%
! CGTAATCGATGGATTTGACG+GGG - Chr8:58844838-58844857 MsG0880045407.01.T01:CDS 45.0%
!! CTTTGAGGCCATTGTGTTGT+TGG + Chr8:58846191-58846210 None:intergenic 45.0%
!! GGTAACGGACTCATTGTAGA+AGG + Chr8:58847427-58847446 None:intergenic 45.0%
ACATATGGCAGCATTGCTGC+AGG + Chr8:58846561-58846580 None:intergenic 50.0%
CACCCTGACCAACAACACAA+TGG - Chr8:58846180-58846199 MsG0880045407.01.T01:intron 50.0%
CACCTGCGGTCTATATTGCA+TGG - Chr8:58846765-58846784 MsG0880045407.01.T01:intron 50.0%
CGAGGCTACTAAGAATCACC+TGG - Chr8:58845180-58845199 MsG0880045407.01.T01:intron 50.0%
CTTCTTGCTCTCGTGAGAGA+AGG + Chr8:58845131-58845150 None:intergenic 50.0%
GAGTGGCTTAGTGTCGTCAT+TGG + Chr8:58846230-58846249 None:intergenic 50.0%
GCTTAATACTACCTCCGTCC+CGG - Chr8:58845422-58845441 MsG0880045407.01.T01:intron 50.0%
GGGACGGAGGTAGTATTAAG+CGG + Chr8:58845423-58845442 None:intergenic 50.0%
GGTGATTCTTAGTAGCCTCG+AGG + Chr8:58845180-58845199 None:intergenic 50.0%
GTCAGTTATTCCGAGACGGA+GGG - Chr8:58845635-58845654 MsG0880045407.01.T01:intron 50.0%
TACAGAGGCCAAGCTTATGC+AGG - Chr8:58846378-58846397 MsG0880045407.01.T01:intron 50.0%
TGAGGTTGGCGCTAGAATGT+TGG - Chr8:58847395-58847414 MsG0880045407.01.T01:CDS 50.0%
TGGTTGCGGCTGCTAGATAA+TGG + Chr8:58844676-58844695 None:intergenic 50.0%
TGTGCGAACCTGCATAAGCT+TGG + Chr8:58846389-58846408 None:intergenic 50.0%
! AGCGGAGTGTCAATGTAGCT+AGG + Chr8:58845405-58845424 None:intergenic 50.0%
! AGGCCATTGTGTTGTTGGTC+AGG + Chr8:58846186-58846205 None:intergenic 50.0%
! ATGGTGACGACGATACACGT+TGG + Chr8:58844657-58844676 None:intergenic 50.0%
! CGCGAGAGCGCAATAATTAC+TGG + Chr8:58844696-58844715 None:intergenic 50.0%
! GGCCATTGTGTTGTTGGTCA+GGG + Chr8:58846185-58846204 None:intergenic 50.0%
! GGTTTTCACTCCGAAACAGG+TGG - Chr8:58844859-58844878 MsG0880045407.01.T01:CDS 50.0%
ACGCCTATCGTCATAGTCCG+TGG + Chr8:58844906-58844925 None:intergenic 55.0%
AGTTGTCCAGAAACGCCTCG+AGG - Chr8:58845162-58845181 MsG0880045407.01.T01:intron 55.0%
ATTGGCGTGGCTTACGGTTG+TGG + Chr8:58846252-58846271 None:intergenic 55.0%
CAACTCCGACCCGTAATCGA+TGG - Chr8:58844827-58844846 MsG0880045407.01.T01:CDS 55.0%
GGTCAATTATACCGGGACGG+AGG + Chr8:58845436-58845455 None:intergenic 55.0%
GGTCAGTTATTCCGAGACGG+AGG - Chr8:58845634-58845653 MsG0880045407.01.T01:intron 55.0%
GTGACGACGATACACGTTGG+AGG + Chr8:58844654-58844673 None:intergenic 55.0%
TCACCTGGAGTACACATCCG+AGG - Chr8:58845195-58845214 MsG0880045407.01.T01:intron 55.0%
! CATCGATTACGGGTCGGAGT+TGG + Chr8:58844829-58844848 None:intergenic 55.0%
! CGGGGTTTTCACTCCGAAAC+AGG - Chr8:58844856-58844875 MsG0880045407.01.T01:CDS 55.0%
! TAGTAGCCTCGAGGCGTTTC+TGG + Chr8:58845171-58845190 None:intergenic 55.0%
! TCGCCACGGACTATGACGAT+AGG - Chr8:58844900-58844919 MsG0880045407.01.T01:CDS 55.0%
CACTGTCAGCCAGTCCGTGT+CGG - Chr8:58846047-58846066 MsG0880045407.01.T01:CDS 60.0%
CGATTACGGGTCGGAGTTGG+TGG + Chr8:58844826-58844845 None:intergenic 60.0%
CGTGGCTTACGGTTGTGGAG+TGG + Chr8:58846247-58846266 None:intergenic 60.0%
GCATAGACTCCGACACGGAC+TGG + Chr8:58846059-58846078 None:intergenic 60.0%
GGTCCTCGGATGTGTACTCC+AGG + Chr8:58845201-58845220 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
Chr8 gene 58844578 58847727 58844578 ID=MsG0880045407.01;Name=MsG0880045407.01
Chr8 mRNA 58844578 58847727 58844578 ID=MsG0880045407.01.T01;Parent=MsG0880045407.01;Name=MsG0880045407.01.T01;_AED=0.49;_eAED=0.52;_QI=204|0.33|0.75|1|1|1|4|221|396
Chr8 exon 58844578 58844950 58844578 ID=MsG0880045407.01.T01:exon:22652;Parent=MsG0880045407.01.T01
Chr8 exon 58845533 58845615 58845533 ID=MsG0880045407.01.T01:exon:22651;Parent=MsG0880045407.01.T01
Chr8 exon 58845718 58846144 58845718 ID=MsG0880045407.01.T01:exon:22650;Parent=MsG0880045407.01.T01
Chr8 exon 58846995 58847727 58846995 ID=MsG0880045407.01.T01:exon:22649;Parent=MsG0880045407.01.T01
Chr8 five_prime_UTR 58847524 58847727 58847524 ID=MsG0880045407.01.T01:five_prime_utr;Parent=MsG0880045407.01.T01
Chr8 CDS 58846995 58847523 58846995 ID=MsG0880045407.01.T01:cds;Parent=MsG0880045407.01.T01
Chr8 CDS 58845718 58846144 58845718 ID=MsG0880045407.01.T01:cds;Parent=MsG0880045407.01.T01
Chr8 CDS 58845533 58845615 58845533 ID=MsG0880045407.01.T01:cds;Parent=MsG0880045407.01.T01
Chr8 CDS 58844799 58844950 58844799 ID=MsG0880045407.01.T01:cds;Parent=MsG0880045407.01.T01
Chr8 three_prime_UTR 58844578 58844798 58844578 ID=MsG0880045407.01.T01:three_prime_utr;Parent=MsG0880045407.01.T01
Gene Sequence

>MsG0880045407.01.T01

ATGGGTGGTCGCAACTGTAATGAAGTTGAGATGAACATCTACCACCAACTCCGACCCGTAATCGATGGATTTGACGGGGTTTTCACTCCGAAACAGGTGGATGCTATAGTCAAGTTTATCGCCACGGACTATGACGATAGGCGTGTTCGTATTCCGCGTGATATTGACGTGATATTTTGTGACGATTTCCCCCTTCTCTCAGCTTATGCAAATCTTAATCGATTGCTGGAGTTTCTAGGTCAGTGTACTCCCATTTCTGTGGTCGATGAACACCTGAATCTGTTTTCTGATTTGATTGGAAAGCTCAAAGTTTACTTTGACGGAGCATGGTTAGATGATTTGTTGACCTTCTCTCACGAGAGCAAGAAGATTCCCGATTTAGTTGTCCAGAAACGCCTCGAGGCTACTAAGAATCACCTGGAGTACACATCCGAGGACCATCGAACTAAACTGAATTATATCAGAGAGACAAGAAAACAGATTCTCGCTGAGATCCAACAAATAAAGGAAGGCAACAAACCCAAAAAAGAGTTCACTATTCTTCTCTTCACCCTGACCAACAACACAATGGCCTCAAAGTCGCATTGTTCTGTGACCAATGACGACACTAAGCCACTCCACAACCGTAAGCCACGCCAATCTTTTGATGATCAGCTTGATGAATTGGAGAGATCTGAACAAGTATTGAGAGAGAGTTTTCGAAACTTGGGCGAGCTTAAAGAGATATTCAACAACATGCTTAAGGATACAGAGGCCAAGCTTATGCAGGTTCGCACAGAGTTGACGACTGTTCTGAAGGCAAGAACCCAAGTATCTTCTCTAGAGTTTGACATTTTTTCTCCCTCCATTGTTCGAAAGAAATCAGAAGAAAGTTGCAAGGCAGAACCTGACCTGTTTCAGAAACATCATGATGATGACTGCTGTAGCCTGCAGCAATGCTGCCATATGTCTTGCAGTATTCTATTTGTTACCTTAATGTGTCTATCTTGTTATGTGGCTTTTCTTGTCATTTATGATAAAGATATTGCTGGCACCTGCGCGAGGAAACTTGGAGGGGCCTTACCAAAAAGTGAACGCATTGAGGTTGGCGCTAGAATGTTGGAAGTAACCTTCTACAATGAGTCCGTTACCAGTACAATTGGAGATGGAGAATCTCAAGAGACATATTTGATTCCCATTTTCATATCATAA
Protein sequence

>MsG0880045407.01.T01

MGGRNCNEVEMNIYHQLRPVIDGFDGVFTPKQVDAIVKFIATDYDDRRVRIPRDIDVIFCDDFPLLSAYANLNRLLEFLGQCTPISVVDEHLNLFSDLIGKLKVYFDGAWLDDLLTFSHESKKIPDLVVQKRLEATKNHLEYTSEDHRTKLNYIRETRKQILAEIQQIKEGNKPKKEFTILLFTLTNNTMASKSHCSVTNDDTKPLHNRKPRQSFDDQLDELERSEQVLRESFRNLGELKEIFNNMLKDTEAKLMQVRTELTTVLKARTQVSSLEFDIFSPSIVRKKSEESCKAEPDLFQKHHDDDCCSLQQCCHMSCSILFVTLMCLSCYVAFLVIYDKDIAGTCARKLGGALPKSERIEVGARMLEVTFYNESVTSTIGDGESQETYLIPIFIS*