AlfalfaGEDB | MsG0880045646.01

M. sativa cultivar ZhongmuNo.1 / MsG0880045646.01

KEGG: -
GO: -
PFAM: PF02704

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880045646.01.T01	XP_003629326.1	97.368	76	2	0	1	76	28	103	8.63E-47	154

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880045646.01.T01	Q93X17	66.667	63	21	0	14	76	42	104	5.96E-23	87

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880045646.01.T01	G7LC52	97.368	76	2	0	1	76	28	103	4.12e-47	154

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0180006028.01	MsG0880045646.01	-0.818584	1.815987e-52	1.364790e-49
MsG0480019812.01	MsG0880045646.01	-0.827853	1.243544e-54	1.215158e-51

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880045646.01.T01	MTR_8g075920	97.368	76	2	0	1	76	28	103	1.04e-50	154
MsG0880045646.01.T01	MTR_3g113890	56.757	74	32	0	3	76	30	103	3.47e-23	85.1
MsG0880045646.01.T01	MTR_1g025220	60.563	71	28	0	6	76	31	101	2.00e-22	82.8
MsG0880045646.01.T01	MTR_4g106860	62.121	66	24	1	12	76	50	115	2.41e-21	80.5
MsG0880045646.01.T01	MTR_1g025250	60.563	71	28	0	6	76	30	100	3.34e-21	79.7
MsG0880045646.01.T01	MTR_8g062800	54.545	66	29	1	12	76	47	112	2.84e-17	70.1
MsG0880045646.01.T01	MTR_1g096370	55.738	61	27	0	16	76	35	95	3.30e-15	64.3

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880045646.01.T01	AT1G75750	63.077	65	24	0	12	76	34	98	1.05e-23	86.3
MsG0880045646.01.T01	AT1G75750	53.947	76	35	0	1	76	22	97	1.08e-23	86.3
MsG0880045646.01.T01	AT2G18420	65.574	61	21	0	16	76	10	70	1.65e-22	82.4
MsG0880045646.01.T01	AT2G18420	65.574	61	21	0	16	76	34	94	2.51e-22	82.4
MsG0880045646.01.T01	AT4G09600	68.852	61	19	0	16	76	39	99	1.13e-21	80.9
MsG0880045646.01.T01	AT1G22690	59.016	61	25	0	16	76	59	119	3.48e-18	72.8
MsG0880045646.01.T01	AT1G22690	59.016	61	25	0	16	76	59	119	3.48e-18	72.8
MsG0880045646.01.T01	AT1G22690	59.016	61	25	0	16	76	59	119	3.48e-18	72.8
MsG0880045646.01.T01	AT5G14920	54.839	62	27	1	16	76	214	275	7.68e-13	61.6
MsG0880045646.01.T01	AT2G14900	38.750	80	42	1	4	76	29	108	1.25e-11	55.8
MsG0880045646.01.T01	AT4G09610	60.656	61	24	0	16	76	39	99	1.44e-11	55.5

Find 20 sgRNAs with CRISPR-Local

Find 26 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CAAGCTCTCTTGCACAGATT+TGG	0.329058	8:-62143468	None:intergenic
AGATTGTGGGGATGCTTGTA+AGG	0.371258	8:+62143422	MsG0880045646.01.T01:intron
TGTGTCCCTCCTGGCACTGC+TGG	0.381275	8:+62143516	MsG0880045646.01.T01:CDS
AACTAACAGATGAGCAACTT+TGG	0.409679	8:+62143265	None:intergenic
GTGTGCAAGTGTGTCCCTCC+TGG	0.412750	8:+62143507	MsG0880045646.01.T01:CDS
TCCCCTCTTCCTCAAACTAT+AGG	0.429732	8:+62143301	MsG0880045646.01.T01:CDS
TTAATATTACCTATAGTTTG+AGG	0.443743	8:-62143310	None:intergenic
TTACCTATAGTTTGAGGAAG+AGG	0.471189	8:-62143304	None:intergenic
GTGTACAATGTATAGATTGT+GGG	0.511353	8:+62143409	MsG0880045646.01.T01:intron
TACCTATAGTTTGAGGAAGA+GGG	0.512116	8:-62143303	None:intergenic
TCAAAGTTTCCAGCAGTGCC+AGG	0.512846	8:-62143525	None:intergenic
GAAGGTGTCTATTATCATCA+AGG	0.572251	8:+62143445	MsG0880045646.01.T01:CDS
GATTGTGGGGATGCTTGTAA+GGG	0.586894	8:+62143423	MsG0880045646.01.T01:intron
GTGGGGATGCTTGTAAGGGA+AGG	0.591141	8:+62143427	MsG0880045646.01.T01:CDS
ACCTATAGTTTGAGGAAGAG+GGG	0.592903	8:-62143302	None:intergenic
AGTTTCCAGCAGTGCCAGGA+GGG	0.604783	8:-62143521	None:intergenic
ATAGTTTGAGGAAGAGGGGA+TGG	0.605727	8:-62143298	None:intergenic
AATCTGTGCAAGAGAGCTTG+TGG	0.624163	8:+62143471	MsG0880045646.01.T01:CDS
AAGTTTCCAGCAGTGCCAGG+AGG	0.683557	8:-62143522	None:intergenic
TGTACAATGTATAGATTGTG+GGG	0.696105	8:+62143410	MsG0880045646.01.T01:intron

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TTAATATTACCTATAGTTTG+AGG	-	Chr8:62143313-62143332	None:intergenic	20.0%
!!!	AAAATAGCTAGTTGATTTAA+TGG	+	Chr8:62143358-62143377	MsG0880045646.01.T01:intron	20.0%
!!!	AAATAGCTAGTTGATTTAAT+GGG	+	Chr8:62143359-62143378	MsG0880045646.01.T01:intron	20.0%
!!!	TTAAATCAACTAGCTATTTT+TGG	-	Chr8:62143358-62143377	None:intergenic	20.0%
!!!	TTATTTAAGAACGTTTGTTA+TGG	+	Chr8:62143387-62143406	MsG0880045646.01.T01:intron	20.0%
	GTGTACAATGTATAGATTGT+GGG	+	Chr8:62143409-62143428	MsG0880045646.01.T01:intron	30.0%
	TGTACAATGTATAGATTGTG+GGG	+	Chr8:62143410-62143429	MsG0880045646.01.T01:intron	30.0%
!	ACACTTTCGAATTTTATTGC+GGG	-	Chr8:62143580-62143599	None:intergenic	30.0%
!	GACACTTTCGAATTTTATTG+CGG	-	Chr8:62143581-62143600	None:intergenic	30.0%
	GGTGTACAATGTATAGATTG+TGG	+	Chr8:62143408-62143427	MsG0880045646.01.T01:intron	35.0%
	TACCTATAGTTTGAGGAAGA+GGG	-	Chr8:62143306-62143325	None:intergenic	35.0%
	TTACCTATAGTTTGAGGAAG+AGG	-	Chr8:62143307-62143326	None:intergenic	35.0%
!!	GAAGGTGTCTATTATCATCA+AGG	+	Chr8:62143445-62143464	MsG0880045646.01.T01:CDS	35.0%
	ACCTATAGTTTGAGGAAGAG+GGG	-	Chr8:62143305-62143324	None:intergenic	40.0%
	AATCTGTGCAAGAGAGCTTG+TGG	+	Chr8:62143471-62143490	MsG0880045646.01.T01:CDS	45.0%
	AGATTGTGGGGATGCTTGTA+AGG	+	Chr8:62143422-62143441	MsG0880045646.01.T01:intron	45.0%
	ATAGTTTGAGGAAGAGGGGA+TGG	-	Chr8:62143301-62143320	None:intergenic	45.0%
	CAAGCTCTCTTGCACAGATT+TGG	-	Chr8:62143471-62143490	None:intergenic	45.0%
	GATTGTGGGGATGCTTGTAA+GGG	+	Chr8:62143423-62143442	MsG0880045646.01.T01:intron	45.0%
	TCCCCTCTTCCTCAAACTAT+AGG	+	Chr8:62143301-62143320	MsG0880045646.01.T01:CDS	45.0%
	TCAAAGTTTCCAGCAGTGCC+AGG	-	Chr8:62143528-62143547	None:intergenic	50.0%
	AAGTTTCCAGCAGTGCCAGG+AGG	-	Chr8:62143525-62143544	None:intergenic	55.0%
	AGTTTCCAGCAGTGCCAGGA+GGG	-	Chr8:62143524-62143543	None:intergenic	55.0%
	GTGGGGATGCTTGTAAGGGA+AGG	+	Chr8:62143427-62143446	MsG0880045646.01.T01:CDS	55.0%
	GTGTGCAAGTGTGTCCCTCC+TGG	+	Chr8:62143507-62143526	MsG0880045646.01.T01:CDS	60.0%
	TGTGTCCCTCCTGGCACTGC+TGG	+	Chr8:62143516-62143535	MsG0880045646.01.T01:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
Chr8	gene	62143274	62143605	62143274	ID=MsG0880045646.01;Name=MsG0880045646.01
Chr8	mRNA	62143274	62143605	62143274	ID=MsG0880045646.01.T01;Parent=MsG0880045646.01;Name=MsG0880045646.01.T01;_AED=0.48;_eAED=0.49;_QI=0\|0\|0\|1\|0\|0\|2\|0\|76
Chr8	exon	62143274	62143322	62143274	ID=MsG0880045646.01.T01:exon:23763;Parent=MsG0880045646.01.T01
Chr8	exon	62143424	62143605	62143424	ID=MsG0880045646.01.T01:exon:23764;Parent=MsG0880045646.01.T01
Chr8	CDS	62143274	62143322	62143274	ID=MsG0880045646.01.T01:cds;Parent=MsG0880045646.01.T01
Chr8	CDS	62143424	62143605	62143424	ID=MsG0880045646.01.T01:cds;Parent=MsG0880045646.01.T01

Gene Sequence	>MsG0880045646.01.T01 ATGAGCAACTTTGGAGCTACATCGCCATCCCCTCTTCCTCAAACTATAGATTGTGGGGATGCTTGTAAGGGAAGGTGTCTATTATCATCAAGGCCAAATCTGTGCAAGAGAGCTTGTGGTAGTTGTTGTGCTGTGTGCAAGTGTGTCCCTCCTGGCACTGCTGGAAACTTTGATTCATGTGAATGTTATGCTAAACTCACAACCCGCAATAAAATTCGAAAGTGTCCTTGA
Protein sequence	>MsG0880045646.01.T01 MSNFGATSPSPLPQTIDCGDACKGRCLLSSRPNLCKRACGSCCAVCKCVPPGTAGNFDSCECYAKLTTRNKIRKCP*