AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0880045908.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880045908.01.T01 MTR_4g094912 96.698 424 14 0 1 424 1 424 0.0 840
MsG0880045908.01.T01 MTR_5g016600 67.972 434 114 8 1 422 1 421 0.0 557
MsG0880045908.01.T01 MTR_3g111060 29.434 265 145 8 156 413 188 417 1.61e-20 93.6
MsG0880045908.01.T01 MTR_3g110142 27.007 274 153 10 152 415 178 414 7.66e-18 85.5
MsG0880045908.01.T01 MTR_3g110142 27.197 239 130 8 152 383 178 379 6.36e-17 82.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880045908.01.T01 AT5G67230 60.641 437 147 9 1 423 1 426 0.0 524
MsG0880045908.01.T01 AT4G36890 61.905 441 145 6 1 423 1 436 0.0 523
MsG0880045908.01.T01 AT1G27600 29.278 263 152 6 156 415 141 372 1.05e-20 94.0
MsG0880045908.01.T01 AT1G27600 29.278 263 152 6 156 415 141 372 1.05e-20 94.0

Find 137 sgRNAs with CRISPR-Local

Find 171 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAATGCGACTTCTTGCTTTA+AGG 0.133453 8:+66275997 MsG0880045908.01.T01:CDS
GCTTCGATTGTTGCCAATTC+AGG 0.223125 8:+66275897 MsG0880045908.01.T01:CDS
AATCTCCGTTAAGCTCTTTC+TGG 0.228040 8:+66275379 MsG0880045908.01.T01:CDS
ACTCAAAGAGAGAGAGAAAT+TGG 0.271072 8:-66275250 None:intergenic
GTGGGAGGGTAGGCATAAAT+TGG 0.292654 8:+66275968 MsG0880045908.01.T01:CDS
TATTCTTCTGAGTCCTGAAT+TGG 0.355170 8:-66275910 None:intergenic
GATGCGGCCACGGCCGGATT+CGG 0.355380 8:-66275647 None:intergenic
CGATAGCAATATGCATAGTA+TGG 0.367152 8:+66276604 MsG0880045908.01.T01:CDS
AAGGATATTAATGCGTTAGA+TGG 0.379320 8:+66276941 MsG0880045908.01.T01:CDS
TGATAAACCAGACTGGATTA+AGG 0.381116 8:+66276922 MsG0880045908.01.T01:CDS
CTGGGTTTGTGTTGAACTCC+AGG 0.389189 8:+66276879 MsG0880045908.01.T01:CDS
TTCATCACCTCATTCGGATT+CGG 0.391988 8:-66275669 None:intergenic
CCTAGGAAGCTTGAATGGTC+TGG 0.401084 8:+66276860 MsG0880045908.01.T01:CDS
TGCCTACCCTCCCACGAAAT+CGG 0.407102 8:-66275960 None:intergenic
GCCGGCACGGGATCCGAATC+CGG 0.407178 8:+66275634 MsG0880045908.01.T01:CDS
GTTTGGATCGTGGTGGAAGC+TGG 0.409365 8:+66275855 MsG0880045908.01.T01:CDS
GATCGCCAACGTTGAGAATC+CGG 0.414086 8:+66275575 MsG0880045908.01.T01:CDS
TGAGCTTTCATCACCTCATT+CGG 0.420016 8:-66275675 None:intergenic
TGTTGAACTCCAGGTTGGTT+TGG 0.428744 8:+66276888 MsG0880045908.01.T01:CDS
CACCGATCAAGTGAAGCTTC+TGG 0.431961 8:-66275797 None:intergenic
GATTCGGATGCGGCCACGGC+CGG 0.435277 8:-66275653 None:intergenic
TTAATATCCTTAATCCAGTC+TGG 0.441071 8:-66276929 None:intergenic
ATGAAATTCAGAGTGTGAAA+TGG 0.442272 8:+66276636 MsG0880045908.01.T01:CDS
TCAGAAGAATAATTCACATT+GGG 0.446964 8:+66275922 MsG0880045908.01.T01:CDS
CCAGACCATTCAAGCTTCCT+AGG 0.449454 8:-66276860 None:intergenic
GAAATGGATAGGGGCAGTTT+CGG 0.452928 8:+66276652 MsG0880045908.01.T01:CDS
ACGGAGCAGAAAGCGTTGTT+CGG 0.455137 8:+66275720 MsG0880045908.01.T01:CDS
CTAGGAAGCTTGAATGGTCT+GGG 0.456470 8:+66276861 MsG0880045908.01.T01:CDS
GTAACTGCAATAACCGTCCT+AGG 0.457737 8:-66275753 None:intergenic
AGGGAAGAACGAACACGGAT+TGG 0.458240 8:-66275540 None:intergenic
TTCGGATTCGGATGCGGCCA+CGG 0.460671 8:-66275657 None:intergenic
TGCCTCATCAGTCTCGCCTT+AGG 0.469549 8:+66275423 MsG0880045908.01.T01:CDS
TTCAACTCCGGCGCCGGCGA+AGG 0.471771 8:+66275504 MsG0880045908.01.T01:CDS
GATTTCACCTTCGCCGGCGC+CGG 0.473327 8:-66275511 None:intergenic
TTGTAATGCGACAAATCATT+TGG 0.479268 8:+66276760 MsG0880045908.01.T01:CDS
GTGTGGTAGTCGGCCGGCAC+GGG 0.480165 8:+66275622 MsG0880045908.01.T01:CDS
GATGCTTGTGCCGTATGATC+TGG 0.482351 8:+66275833 MsG0880045908.01.T01:CDS
GATAATTGAAAGAGTTCAAA+CGG 0.482387 8:+66275701 MsG0880045908.01.T01:CDS
AATGCGACAAATCATTTGGT+TGG 0.484604 8:+66276764 MsG0880045908.01.T01:CDS
TGGATAGGGGCAGTTTCGGT+TGG 0.485363 8:+66276656 MsG0880045908.01.T01:CDS
AATTGTGAGAAAAGAGAAGC+TGG 0.486760 8:+66276559 MsG0880045908.01.T01:CDS
TGAGAAAAGAGAAGCTGGAT+GGG 0.487150 8:+66276564 MsG0880045908.01.T01:CDS
CTTTGATGCTGTTGCTCCGG+CGG 0.487489 8:-66275317 None:intergenic
GCCGGATTCGGATCCCGTGC+CGG 0.488927 8:-66275635 None:intergenic
AAGAGCCAGAAAGAGCTTAA+CGG 0.494365 8:-66275384 None:intergenic
TTCAGAGTGTGAAATGGATA+GGG 0.499356 8:+66276642 MsG0880045908.01.T01:CDS
GTGCAGTGTACATAGGTGAC+AGG 0.505484 8:+66276825 MsG0880045908.01.T01:CDS
ATGCGACAAATCATTTGGTT+GGG 0.508255 8:+66276765 MsG0880045908.01.T01:CDS
GGACCTTGTACTGGCATTGG+CGG 0.508552 8:-66276734 None:intergenic
TTCACATTGGGTTTAATCAA+CGG 0.508584 8:+66275934 MsG0880045908.01.T01:CDS
ACGGGATCCGAATCCGGCCG+TGG 0.508857 8:+66275640 MsG0880045908.01.T01:CDS
GCAGCCACCAAAGCAAAACT+TGG 0.510346 8:-66277042 None:intergenic
AACGGAGATTTCACCTTCGC+CGG 0.510863 8:-66275517 None:intergenic
CGTGTGGTAGTCGGCCGGCA+CGG 0.511288 8:+66275621 MsG0880045908.01.T01:CDS
TTCCAGAAGCTTCACTTGAT+CGG 0.512321 8:+66275795 MsG0880045908.01.T01:CDS
GTTCCGTTCAACTCCGGCGC+CGG 0.512508 8:+66275498 MsG0880045908.01.T01:CDS
GAACTCCAGGTTGGTTTGGA+AGG 0.512764 8:+66276892 MsG0880045908.01.T01:CDS
TCCACCGTTCCGTTCAACTC+CGG 0.515133 8:+66275492 MsG0880045908.01.T01:CDS
ATTCAGAGTGTGAAATGGAT+AGG 0.518842 8:+66276641 MsG0880045908.01.T01:CDS
CCGCCGCTGTTTGAATCCTC+CGG 0.522304 8:+66275342 MsG0880045908.01.T01:CDS
TGATACCTAGGAAGCTTGAA+TGG 0.523545 8:+66276855 MsG0880045908.01.T01:CDS
CCGGTGAGCCGTGTGGTAGT+CGG 0.524693 8:+66275612 MsG0880045908.01.T01:CDS
TTGTGCCGTATGATCTGGTT+TGG 0.525860 8:+66275838 MsG0880045908.01.T01:CDS
ATCAACGGATTCCGATTTCG+TGG 0.526508 8:+66275949 MsG0880045908.01.T01:CDS
GGAGTTGAACGGAACGGTGG+AGG 0.530558 8:-66275490 None:intergenic
ACGGATTCCGATTTCGTGGG+AGG 0.534517 8:+66275953 MsG0880045908.01.T01:CDS
ACCTCATTCGGATTCGGATG+CGG 0.535206 8:-66275663 None:intergenic
GCACCGCCAATGCCAGTACA+AGG 0.536252 8:+66276731 MsG0880045908.01.T01:CDS
GAATCCTCCGGCGACATCAC+CGG 0.538522 8:+66275354 MsG0880045908.01.T01:CDS
GTGAGAAAAGAGAAGCTGGA+TGG 0.544797 8:+66276563 MsG0880045908.01.T01:CDS
GTTTGTGTTGAACTCCAGGT+TGG 0.547756 8:+66276883 MsG0880045908.01.T01:CDS
TGATTTGTCGCATTACAAGC+AGG 0.549322 8:-66276755 None:intergenic
CTCAGAAGAATAATTCACAT+TGG 0.550439 8:+66275921 MsG0880045908.01.T01:CDS
GGATGGGATTGTGATGTTTG+CGG 0.551509 8:+66276580 MsG0880045908.01.T01:CDS
TCAAGCTTCCTAGGTATCAC+AGG 0.552925 8:-66276851 None:intergenic
ATCTGTGTGTTAGAACCACT+TGG 0.553300 8:+66277007 MsG0880045908.01.T01:CDS
CAGTGAGGTTGAAGATTCAT+CGG 0.554218 8:-66276699 None:intergenic
CAAGAGAATACTCACTGAGG+TGG 0.554864 8:-66277094 None:intergenic
GGATTCTCAACGTTGGCGAT+CGG 0.556565 8:-66275573 None:intergenic
GTTCTTTGATGCTGTTGCTC+CGG 0.560583 8:-66275320 None:intergenic
TTAGAACCACTTGGGAGTTG+TGG 0.565178 8:+66277016 MsG0880045908.01.T01:CDS
TTACAAGCAGGACCTTGTAC+TGG 0.565934 8:-66276743 None:intergenic
GCAGGACCTTGTACTGGCAT+TGG 0.568016 8:-66276737 None:intergenic
TCGCCGGCGCCGGAGTTGAA+CGG 0.570510 8:-66275501 None:intergenic
AATATCCTTCCAAACCAACC+TGG 0.570735 8:-66276897 None:intergenic
AGGAGAGGTGCAGTGTACAT+AGG 0.570975 8:+66276818 MsG0880045908.01.T01:CDS
GAGGAGCAGAAAGAAGATGC+GGG 0.572453 8:-66275457 None:intergenic
TGAATCTTCAACCTCACTGA+AGG 0.574930 8:+66276703 MsG0880045908.01.T01:CDS
ACGATCCAAACCAGATCATA+CGG 0.577778 8:-66275843 None:intergenic
GCGGGAGATACGAAATCCTA+AGG 0.578276 8:-66275439 None:intergenic
TGAGCCGTGTGGTAGTCGGC+CGG 0.578753 8:+66275616 MsG0880045908.01.T01:CDS
TCAACGGATTCCGATTTCGT+GGG 0.578860 8:+66275950 MsG0880045908.01.T01:CDS
GGCGAGACTGATGAGGCAGA+AGG 0.582525 8:-66275418 None:intergenic
AGAACGAACACGGATTGGAA+CGG 0.583542 8:-66275535 None:intergenic
TGTTCGGAGTGAAGAACCCT+AGG 0.584133 8:+66275736 MsG0880045908.01.T01:CDS
CGGTGGAGGAAGTTGAGAAG+AGG 0.585791 8:-66275476 None:intergenic
CAACATTGTTACAGAACCGC+CGG 0.588448 8:+66275301 MsG0880045908.01.T01:CDS
CGGATTCCGATTTCGTGGGA+GGG 0.589469 8:+66275954 MsG0880045908.01.T01:CDS
TGATGAGGCAGAAGGCAGCA+TGG 0.589879 8:-66275410 None:intergenic
AGAGGAGCAGAAAGAAGATG+CGG 0.590320 8:-66275458 None:intergenic
CCGACTACCACACGGCTCAC+CGG 0.590866 8:-66275612 None:intergenic
AAGAGAATACTCACTGAGGT+GGG 0.593078 8:-66277093 None:intergenic
GGCGATCGGATTCACAGCGA+GGG 0.595287 8:-66275559 None:intergenic
GCCGCATCCGAATCCGAATG+AGG 0.596142 8:+66275662 MsG0880045908.01.T01:CDS
ATCTTCAACCTCACTGAAGG+AGG 0.596449 8:+66276706 MsG0880045908.01.T01:CDS
TTCCACCGGATTCTCAACGT+TGG 0.599194 8:-66275580 None:intergenic
ATGATTCCTTAAAATATGCA+AGG 0.603247 8:+66276798 MsG0880045908.01.T01:CDS
TTCAACCTCACTGAAGGAGG+AGG 0.604655 8:+66276709 MsG0880045908.01.T01:CDS
ACAGGGCACCTGTGATACCT+AGG 0.606839 8:+66276843 MsG0880045908.01.T01:CDS
TTCCGATTTCGTGGGAGGGT+AGG 0.613740 8:+66275958 MsG0880045908.01.T01:CDS
GTATGATCTGGTTTGGATCG+TGG 0.620534 8:+66275845 MsG0880045908.01.T01:CDS
CAGTTACGCCGACGTATGTG+AGG 0.628360 8:+66275769 MsG0880045908.01.T01:CDS
TCCTTAAAATATGCAAGGAG+AGG 0.630960 8:+66276803 MsG0880045908.01.T01:CDS
TGGATCGTGGTGGAAGCTGG+AGG 0.631658 8:+66275858 MsG0880045908.01.T01:CDS
CAGCGAGGGAAGAACGAACA+CGG 0.641810 8:-66275545 None:intergenic
ATGTCTTTCAGCAGAGACAG+TGG 0.642175 8:-66276986 None:intergenic
ATATTGATGATAAACCAGAC+TGG 0.644473 8:+66276915 MsG0880045908.01.T01:CDS
TCAGAGTGTGAAATGGATAG+GGG 0.644914 8:+66276643 MsG0880045908.01.T01:CDS
CGGTGCCTCCTCCTTCAGTG+AGG 0.650992 8:-66276714 None:intergenic
TAACTGCAATAACCGTCCTA+GGG 0.651011 8:-66275752 None:intergenic
ATCCTAAGGCGAGACTGATG+AGG 0.654027 8:-66275425 None:intergenic
CGTGCCGGCCGACTACCACA+CGG 0.661754 8:-66275620 None:intergenic
TGCAGTGTACATAGGTGACA+GGG 0.667397 8:+66276826 MsG0880045908.01.T01:CDS
CGGAGTGAAGAACCCTAGGA+CGG 0.669685 8:+66275740 MsG0880045908.01.T01:CDS
GGCGCCGGAGTTGAACGGAA+CGG 0.674192 8:-66275496 None:intergenic
TGGCGATCGGATTCACAGCG+AGG 0.675052 8:-66275560 None:intergenic
CGCCAACGTTGAGAATCCGG+TGG 0.680326 8:+66275578 MsG0880045908.01.T01:CDS
CCGGAGGATTCAAACAGCGG+CGG 0.682243 8:-66275342 None:intergenic
TCGCCGGAGGATTCAAACAG+CGG 0.693324 8:-66275345 None:intergenic
TCTGTGTGTTAGAACCACTT+GGG 0.693971 8:+66277008 MsG0880045908.01.T01:CDS
TGATCTGGTTTGGATCGTGG+TGG 0.700689 8:+66275848 MsG0880045908.01.T01:CDS
TGAGGCAGAAGGCAGCATGG+AGG 0.702139 8:-66275407 None:intergenic
GCCGGAGTTGAACGGAACGG+TGG 0.702755 8:-66275493 None:intergenic
AGTAGCTCCGGTGAGCCGTG+TGG 0.704787 8:+66275605 MsG0880045908.01.T01:CDS
TGGCGTCCACAACTCCCAAG+TGG 0.709260 8:-66277022 None:intergenic
AATCAAGAGAATACTCACTG+AGG 0.750028 8:-66277097 None:intergenic
TGGAACGTCCTCACATACGT+CGG 0.783371 8:-66275777 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATATCATTTTCTCAAATTAT+TGG + Chr8:66276226-66276245 MsG0880045908.01.T01:intron 15.0%
!! AAACTAAAAAATCATATGAG+TGG - Chr8:66276461-66276480 None:intergenic 20.0%
!! TAAGCAAAAAACAAGTTTAT+AGG - Chr8:66276356-66276375 None:intergenic 20.0%
!! TGAAAATTTAGTTGATTGAT+AGG + Chr8:66276501-66276520 MsG0880045908.01.T01:intron 20.0%
!!! AAAACAACATTTTAAGTATC+TGG + Chr8:66276292-66276311 MsG0880045908.01.T01:intron 20.0%
!!! TTTAAGGTATATTCCAATTT+TGG + Chr8:66276013-66276032 MsG0880045908.01.T01:intron 20.0%
! ATGATTCCTTAAAATATGCA+AGG + Chr8:66276798-66276817 MsG0880045908.01.T01:CDS 25.0%
! CATTGAATAACTAATGTATC+TGG - Chr8:66276331-66276350 None:intergenic 25.0%
! GATAATTGAAAGAGTTCAAA+CGG + Chr8:66275701-66275720 MsG0880045908.01.T01:CDS 25.0%
! TCAGAAGAATAATTCACATT+GGG + Chr8:66275922-66275941 MsG0880045908.01.T01:CDS 25.0%
!! TTTTTGCTTATAAATGTGAC+AGG + Chr8:66276366-66276385 MsG0880045908.01.T01:intron 25.0%
!!! TATTCCAATTTTGGAGATAT+TGG + Chr8:66276022-66276041 MsG0880045908.01.T01:intron 25.0%
!!! TCAAAAAAAGTATTGTGTCA+CGG - Chr8:66276161-66276180 None:intergenic 25.0%
AAGGATATTAATGCGTTAGA+TGG + Chr8:66276941-66276960 MsG0880045908.01.T01:CDS 30.0%
ACAATTGATTGTTTGCAATG+TGG + Chr8:66276528-66276547 MsG0880045908.01.T01:intron 30.0%
AGAATATAATAGTGACCAGA+GGG + Chr8:66276398-66276417 MsG0880045908.01.T01:intron 30.0%
ATGAAATTCAGAGTGTGAAA+TGG + Chr8:66276636-66276655 MsG0880045908.01.T01:CDS 30.0%
CTCAGAAGAATAATTCACAT+TGG + Chr8:66275921-66275940 MsG0880045908.01.T01:CDS 30.0%
TCAACCAATATCTCCAAAAT+TGG - Chr8:66276029-66276048 None:intergenic 30.0%
TTAATATCCTTAATCCAGTC+TGG - Chr8:66276932-66276951 None:intergenic 30.0%
TTCACATTGGGTTTAATCAA+CGG + Chr8:66275934-66275953 MsG0880045908.01.T01:CDS 30.0%
TTGTAATGCGACAAATCATT+TGG + Chr8:66276760-66276779 MsG0880045908.01.T01:CDS 30.0%
! ATATTGATGATAAACCAGAC+TGG + Chr8:66276915-66276934 MsG0880045908.01.T01:CDS 30.0%
!!! TGACTTTTTTCACTAGTTTG+CGG + Chr8:66276046-66276065 MsG0880045908.01.T01:intron 30.0%
AATGCGACAAATCATTTGGT+TGG + Chr8:66276764-66276783 MsG0880045908.01.T01:CDS 35.0%
AATTGTGAGAAAAGAGAAGC+TGG + Chr8:66276559-66276578 MsG0880045908.01.T01:CDS 35.0%
ACTCAAAGAGAGAGAGAAAT+TGG - Chr8:66275253-66275272 None:intergenic 35.0%
ATGCGACAAATCATTTGGTT+GGG + Chr8:66276765-66276784 MsG0880045908.01.T01:CDS 35.0%
ATTCAGAGTGTGAAATGGAT+AGG + Chr8:66276641-66276660 MsG0880045908.01.T01:CDS 35.0%
ATTGATTGTTTGCAATGTGG+TGG + Chr8:66276531-66276550 MsG0880045908.01.T01:intron 35.0%
CATGTGTTAGTATTGTGTCA+CGG - Chr8:66276191-66276210 None:intergenic 35.0%
CGATAGCAATATGCATAGTA+TGG + Chr8:66276604-66276623 MsG0880045908.01.T01:CDS 35.0%
GAGAATATAATAGTGACCAG+AGG + Chr8:66276397-66276416 MsG0880045908.01.T01:intron 35.0%
TATTCTTCTGAGTCCTGAAT+TGG - Chr8:66275913-66275932 None:intergenic 35.0%
TCCTTAAAATATGCAAGGAG+AGG + Chr8:66276803-66276822 MsG0880045908.01.T01:CDS 35.0%
TGATAAACCAGACTGGATTA+AGG + Chr8:66276922-66276941 MsG0880045908.01.T01:CDS 35.0%
TGCTTATAAATGTGACAGGA+GGG + Chr8:66276370-66276389 MsG0880045908.01.T01:intron 35.0%
TTCAGAGTGTGAAATGGATA+GGG + Chr8:66276642-66276661 MsG0880045908.01.T01:CDS 35.0%
TTGCTTATAAATGTGACAGG+AGG + Chr8:66276369-66276388 MsG0880045908.01.T01:intron 35.0%
! ACCTCTCCTTGCATATTTTA+AGG - Chr8:66276807-66276826 None:intergenic 35.0%
!! TTTTCACTAGTTTGCGGATA+AGG + Chr8:66276052-66276071 MsG0880045908.01.T01:intron 35.0%
AAGAGCCAGAAAGAGCTTAA+CGG - Chr8:66275387-66275406 None:intergenic 40.0%
AATATCCTTCCAAACCAACC+TGG - Chr8:66276900-66276919 None:intergenic 40.0%
AATCTCCGTTAAGCTCTTTC+TGG + Chr8:66275379-66275398 MsG0880045908.01.T01:CDS 40.0%
ACGATCCAAACCAGATCATA+CGG - Chr8:66275846-66275865 None:intergenic 40.0%
ATCTGTGTGTTAGAACCACT+TGG + Chr8:66277007-66277026 MsG0880045908.01.T01:CDS 40.0%
CACTTACATGTATCTGACAC+TGG - Chr8:66276131-66276150 None:intergenic 40.0%
CAGTGAGGTTGAAGATTCAT+CGG - Chr8:66276702-66276721 None:intergenic 40.0%
CTATAATGAACTACTCCCTC+TGG - Chr8:66276416-66276435 None:intergenic 40.0%
GAATGCGACTTCTTGCTTTA+AGG + Chr8:66275997-66276016 MsG0880045908.01.T01:CDS 40.0%
TAACTGCAATAACCGTCCTA+GGG - Chr8:66275755-66275774 None:intergenic 40.0%
TCAGAGTGTGAAATGGATAG+GGG + Chr8:66276643-66276662 MsG0880045908.01.T01:CDS 40.0%
TCATGAACAATACTTCCCCT+GGG - Chr8:66276268-66276287 None:intergenic 40.0%
TCTGTGTGTTAGAACCACTT+GGG + Chr8:66277008-66277027 MsG0880045908.01.T01:CDS 40.0%
TGAATCTTCAACCTCACTGA+AGG + Chr8:66276703-66276722 MsG0880045908.01.T01:CDS 40.0%
TGAGAAAAGAGAAGCTGGAT+GGG + Chr8:66276564-66276583 MsG0880045908.01.T01:CDS 40.0%
TGAGCTTTCATCACCTCATT+CGG - Chr8:66275678-66275697 None:intergenic 40.0%
TGATACCTAGGAAGCTTGAA+TGG + Chr8:66276855-66276874 MsG0880045908.01.T01:CDS 40.0%
TTCATCACCTCATTCGGATT+CGG - Chr8:66275672-66275691 None:intergenic 40.0%
TTCCAGAAGCTTCACTTGAT+CGG + Chr8:66275795-66275814 MsG0880045908.01.T01:CDS 40.0%
! AGAGCTTAACGGAGATTTTC+CGG - Chr8:66275376-66275395 None:intergenic 40.0%
! TGATTTGTCGCATTACAAGC+AGG - Chr8:66276758-66276777 None:intergenic 40.0%
AGAGGAGCAGAAAGAAGATG+CGG - Chr8:66275461-66275480 None:intergenic 45.0%
ATCTTCAACCTCACTGAAGG+AGG + Chr8:66276706-66276725 MsG0880045908.01.T01:CDS 45.0%
ATGTCTTTCAGCAGAGACAG+TGG - Chr8:66276989-66277008 None:intergenic 45.0%
CAACATTGTTACAGAACCGC+CGG + Chr8:66275301-66275320 MsG0880045908.01.T01:CDS 45.0%
CTAGGAAGCTTGAATGGTCT+GGG + Chr8:66276861-66276880 MsG0880045908.01.T01:CDS 45.0%
CTCATGAACAATACTTCCCC+TGG - Chr8:66276269-66276288 None:intergenic 45.0%
GAAATGGATAGGGGCAGTTT+CGG + Chr8:66276652-66276671 MsG0880045908.01.T01:CDS 45.0%
GCTTCGATTGTTGCCAATTC+AGG + Chr8:66275897-66275916 MsG0880045908.01.T01:CDS 45.0%
GGATGGGATTGTGATGTTTG+CGG + Chr8:66276580-66276599 MsG0880045908.01.T01:CDS 45.0%
GTAACTGCAATAACCGTCCT+AGG - Chr8:66275756-66275775 None:intergenic 45.0%
GTGAGAAAAGAGAAGCTGGA+TGG + Chr8:66276563-66276582 MsG0880045908.01.T01:CDS 45.0%
GTTCTTTGATGCTGTTGCTC+CGG - Chr8:66275323-66275342 None:intergenic 45.0%
GTTTGTGTTGAACTCCAGGT+TGG + Chr8:66276883-66276902 MsG0880045908.01.T01:CDS 45.0%
TCAAGCTTCCTAGGTATCAC+AGG - Chr8:66276854-66276873 None:intergenic 45.0%
TGCAGTGTACATAGGTGACA+GGG + Chr8:66276826-66276845 MsG0880045908.01.T01:CDS 45.0%
TGTCAATATGTTAGCGCCCA+GGG + Chr8:66276249-66276268 MsG0880045908.01.T01:intron 45.0%
TTACAAGCAGGACCTTGTAC+TGG - Chr8:66276746-66276765 None:intergenic 45.0%
TTAGAACCACTTGGGAGTTG+TGG + Chr8:66277016-66277035 MsG0880045908.01.T01:CDS 45.0%
TTGTGCCGTATGATCTGGTT+TGG + Chr8:66275838-66275857 MsG0880045908.01.T01:CDS 45.0%
! ATCAACGGATTCCGATTTCG+TGG + Chr8:66275949-66275968 MsG0880045908.01.T01:CDS 45.0%
! TCAACGGATTCCGATTTCGT+GGG + Chr8:66275950-66275969 MsG0880045908.01.T01:CDS 45.0%
!! AAGTTTTGCTTTGGTGGCTG+CGG + Chr8:66277044-66277063 MsG0880045908.01.T01:CDS 45.0%
!! AGAACGAACACGGATTGGAA+CGG - Chr8:66275538-66275557 None:intergenic 45.0%
!! GTATGATCTGGTTTGGATCG+TGG + Chr8:66275845-66275864 MsG0880045908.01.T01:CDS 45.0%
!! TGTTGAACTCCAGGTTGGTT+TGG + Chr8:66276888-66276907 MsG0880045908.01.T01:CDS 45.0%
AACGGAGATTTCACCTTCGC+CGG - Chr8:66275520-66275539 None:intergenic 50.0%
ACCTCATTCGGATTCGGATG+CGG - Chr8:66275666-66275685 None:intergenic 50.0%
AGGGAAGAACGAACACGGAT+TGG - Chr8:66275543-66275562 None:intergenic 50.0%
ATCCTAAGGCGAGACTGATG+AGG - Chr8:66275428-66275447 None:intergenic 50.0%
CACCGATCAAGTGAAGCTTC+TGG - Chr8:66275800-66275819 None:intergenic 50.0%
CCAGACCATTCAAGCTTCCT+AGG - Chr8:66276863-66276882 None:intergenic 50.0%
CCTAGGAAGCTTGAATGGTC+TGG + Chr8:66276860-66276879 MsG0880045908.01.T01:CDS 50.0%
CTGGGTTTGTGTTGAACTCC+AGG + Chr8:66276879-66276898 MsG0880045908.01.T01:CDS 50.0%
GAGGAGCAGAAAGAAGATGC+GGG - Chr8:66275460-66275479 None:intergenic 50.0%
GATCGCCAACGTTGAGAATC+CGG + Chr8:66275575-66275594 MsG0880045908.01.T01:CDS 50.0%
GATGCTTGTGCCGTATGATC+TGG + Chr8:66275833-66275852 MsG0880045908.01.T01:CDS 50.0%
GCAGCCACCAAAGCAAAACT+TGG - Chr8:66277045-66277064 None:intergenic 50.0%
GCGGGAGATACGAAATCCTA+AGG - Chr8:66275442-66275461 None:intergenic 50.0%
GGATTCTCAACGTTGGCGAT+CGG - Chr8:66275576-66275595 None:intergenic 50.0%
GTCAATATGTTAGCGCCCAG+GGG + Chr8:66276250-66276269 MsG0880045908.01.T01:intron 50.0%
GTGCAGTGTACATAGGTGAC+AGG + Chr8:66276825-66276844 MsG0880045908.01.T01:CDS 50.0%
GTGGGAGGGTAGGCATAAAT+TGG + Chr8:66275968-66275987 MsG0880045908.01.T01:CDS 50.0%
GTGTCAATATGTTAGCGCCC+AGG + Chr8:66276248-66276267 MsG0880045908.01.T01:intron 50.0%
TCCGGTGGAAAAAGTAGCTC+CGG + Chr8:66275593-66275612 MsG0880045908.01.T01:CDS 50.0%
TGGAACGTCCTCACATACGT+CGG - Chr8:66275780-66275799 None:intergenic 50.0%
TGTTCGGAGTGAAGAACCCT+AGG + Chr8:66275736-66275755 MsG0880045908.01.T01:CDS 50.0%
TTCAACCTCACTGAAGGAGG+AGG + Chr8:66276709-66276728 MsG0880045908.01.T01:CDS 50.0%
TTCCACCGGATTCTCAACGT+TGG - Chr8:66275583-66275602 None:intergenic 50.0%
! AGATTTTCCGGTGATGTCGC+CGG - Chr8:66275364-66275383 None:intergenic 50.0%
! AGGAGAGGTGCAGTGTACAT+AGG + Chr8:66276818-66276837 MsG0880045908.01.T01:CDS 50.0%
! GACGCCAAGTTTTGCTTTGG+TGG + Chr8:66277038-66277057 MsG0880045908.01.T01:CDS 50.0%
! GTGGACGCCAAGTTTTGCTT+TGG + Chr8:66277035-66277054 MsG0880045908.01.T01:CDS 50.0%
!! ACGGAGCAGAAAGCGTTGTT+CGG + Chr8:66275720-66275739 MsG0880045908.01.T01:CDS 50.0%
!! AGTTTTGCTTTGGTGGCTGC+GGG + Chr8:66277045-66277064 MsG0880045908.01.T01:CDS 50.0%
!! GAACTCCAGGTTGGTTTGGA+AGG + Chr8:66276892-66276911 MsG0880045908.01.T01:CDS 50.0%
!! TGATCTGGTTTGGATCGTGG+TGG + Chr8:66275848-66275867 MsG0880045908.01.T01:CDS 50.0%
!!! ACCGGAGCTACTTTTTCCAC+CGG - Chr8:66275597-66275616 None:intergenic 50.0%
ACGGATTCCGATTTCGTGGG+AGG + Chr8:66275953-66275972 MsG0880045908.01.T01:CDS 55.0%
CAGCGAGGGAAGAACGAACA+CGG - Chr8:66275548-66275567 None:intergenic 55.0%
CAGTTACGCCGACGTATGTG+AGG + Chr8:66275769-66275788 MsG0880045908.01.T01:CDS 55.0%
CGGAGTGAAGAACCCTAGGA+CGG + Chr8:66275740-66275759 MsG0880045908.01.T01:CDS 55.0%
CGGATTCCGATTTCGTGGGA+GGG + Chr8:66275954-66275973 MsG0880045908.01.T01:CDS 55.0%
CGGTGGAGGAAGTTGAGAAG+AGG - Chr8:66275479-66275498 None:intergenic 55.0%
CTTTGATGCTGTTGCTCCGG+CGG - Chr8:66275320-66275339 None:intergenic 55.0%
TCCACCGTTCCGTTCAACTC+CGG + Chr8:66275492-66275511 MsG0880045908.01.T01:CDS 55.0%
TCGCCGGAGGATTCAAACAG+CGG - Chr8:66275348-66275367 None:intergenic 55.0%
TGATGAGGCAGAAGGCAGCA+TGG - Chr8:66275413-66275432 None:intergenic 55.0%
TGCCTACCCTCCCACGAAAT+CGG - Chr8:66275963-66275982 None:intergenic 55.0%
TGCCTCATCAGTCTCGCCTT+AGG + Chr8:66275423-66275442 MsG0880045908.01.T01:CDS 55.0%
TGGATAGGGGCAGTTTCGGT+TGG + Chr8:66276656-66276675 MsG0880045908.01.T01:CDS 55.0%
TTCCGATTTCGTGGGAGGGT+AGG + Chr8:66275958-66275977 MsG0880045908.01.T01:CDS 55.0%
! ACAGGGCACCTGTGATACCT+AGG + Chr8:66276843-66276862 MsG0880045908.01.T01:CDS 55.0%
! GCAGGACCTTGTACTGGCAT+TGG - Chr8:66276740-66276759 None:intergenic 55.0%
! GGACCTTGTACTGGCATTGG+CGG - Chr8:66276737-66276756 None:intergenic 55.0%
! GTTTGGATCGTGGTGGAAGC+TGG + Chr8:66275855-66275874 MsG0880045908.01.T01:CDS 55.0%
CCGCCGCTGTTTGAATCCTC+CGG + Chr8:66275342-66275361 MsG0880045908.01.T01:CDS 60.0%
CCGGAGGATTCAAACAGCGG+CGG - Chr8:66275345-66275364 None:intergenic 60.0%
CGCCAACGTTGAGAATCCGG+TGG + Chr8:66275578-66275597 MsG0880045908.01.T01:CDS 60.0%
GAATCCTCCGGCGACATCAC+CGG + Chr8:66275354-66275373 MsG0880045908.01.T01:CDS 60.0%
GCACCGCCAATGCCAGTACA+AGG + Chr8:66276731-66276750 MsG0880045908.01.T01:CDS 60.0%
GCCGCATCCGAATCCGAATG+AGG + Chr8:66275662-66275681 MsG0880045908.01.T01:CDS 60.0%
GGAGTTGAACGGAACGGTGG+AGG - Chr8:66275493-66275512 None:intergenic 60.0%
GGCGAGACTGATGAGGCAGA+AGG - Chr8:66275421-66275440 None:intergenic 60.0%
GGCGATCGGATTCACAGCGA+GGG - Chr8:66275562-66275581 None:intergenic 60.0%
TGAGGCAGAAGGCAGCATGG+AGG - Chr8:66275410-66275429 None:intergenic 60.0%
TGGCGATCGGATTCACAGCG+AGG - Chr8:66275563-66275582 None:intergenic 60.0%
TGGCGTCCACAACTCCCAAG+TGG - Chr8:66277025-66277044 None:intergenic 60.0%
TTCGGATTCGGATGCGGCCA+CGG - Chr8:66275660-66275679 None:intergenic 60.0%
! TGGATCGTGGTGGAAGCTGG+AGG + Chr8:66275858-66275877 MsG0880045908.01.T01:CDS 60.0%
! TTTTCCGGTGATGTCGCCGG+AGG - Chr8:66275361-66275380 None:intergenic 60.0%
AGTAGCTCCGGTGAGCCGTG+TGG + Chr8:66275605-66275624 MsG0880045908.01.T01:CDS 65.0%
CCGACTACCACACGGCTCAC+CGG - Chr8:66275615-66275634 None:intergenic 65.0%
CCGGTGAGCCGTGTGGTAGT+CGG + Chr8:66275612-66275631 MsG0880045908.01.T01:CDS 65.0%
CGGTGCCTCCTCCTTCAGTG+AGG - Chr8:66276717-66276736 None:intergenic 65.0%
GATTTCACCTTCGCCGGCGC+CGG - Chr8:66275514-66275533 None:intergenic 65.0%
GCCGGAGTTGAACGGAACGG+TGG - Chr8:66275496-66275515 None:intergenic 65.0%
GTTCCGTTCAACTCCGGCGC+CGG + Chr8:66275498-66275517 MsG0880045908.01.T01:CDS 65.0%
TGAGCCGTGTGGTAGTCGGC+CGG + Chr8:66275616-66275635 MsG0880045908.01.T01:CDS 65.0%
! GGCGCCGGAGTTGAACGGAA+CGG - Chr8:66275499-66275518 None:intergenic 65.0%
ACGGGATCCGAATCCGGCCG+TGG + Chr8:66275640-66275659 MsG0880045908.01.T01:CDS 70.0%
CGTGTGGTAGTCGGCCGGCA+CGG + Chr8:66275621-66275640 MsG0880045908.01.T01:CDS 70.0%
GATGCGGCCACGGCCGGATT+CGG - Chr8:66275650-66275669 None:intergenic 70.0%
GATTCGGATGCGGCCACGGC+CGG - Chr8:66275656-66275675 None:intergenic 70.0%
GCCGGATTCGGATCCCGTGC+CGG - Chr8:66275638-66275657 None:intergenic 70.0%
GCCGGCACGGGATCCGAATC+CGG + Chr8:66275634-66275653 MsG0880045908.01.T01:CDS 70.0%
GTGTGGTAGTCGGCCGGCAC+GGG + Chr8:66275622-66275641 MsG0880045908.01.T01:CDS 70.0%
! CGTGCCGGCCGACTACCACA+CGG - Chr8:66275623-66275642 None:intergenic 70.0%
! TCGCCGGCGCCGGAGTTGAA+CGG - Chr8:66275504-66275523 None:intergenic 70.0%
!! TTCAACTCCGGCGCCGGCGA+AGG + Chr8:66275504-66275523 MsG0880045908.01.T01:CDS 70.0%
Chromosome Type Strat End Strand Name
Chr8 gene 66275224 66277105 66275224 ID=MsG0880045908.01;Name=MsG0880045908.01
Chr8 mRNA 66275224 66277105 66275224 ID=MsG0880045908.01.T01;Parent=MsG0880045908.01;Name=MsG0880045908.01.T01;_AED=0.49;_eAED=0.49;_QI=67|1|0.5|1|1|1|2|0|424
Chr8 exon 66275224 66276018 66275224 ID=MsG0880045908.01.T01:exon:25237;Parent=MsG0880045908.01.T01
Chr8 exon 66276559 66277105 66276559 ID=MsG0880045908.01.T01:exon:25238;Parent=MsG0880045908.01.T01
Chr8 five_prime_UTR 66275224 66275290 66275224 ID=MsG0880045908.01.T01:five_prime_utr;Parent=MsG0880045908.01.T01
Chr8 CDS 66275291 66276018 66275291 ID=MsG0880045908.01.T01:cds;Parent=MsG0880045908.01.T01
Chr8 CDS 66276559 66277105 66276559 ID=MsG0880045908.01.T01:cds;Parent=MsG0880045908.01.T01
Gene Sequence

>MsG0880045908.01.T01

ATGAAACTCTCAACATTGTTACAGAACCGCCGGAGCAACAGCATCAAAGAACCGCCGCTGTTTGAATCCTCCGGCGACATCACCGGAAAATCTCCGTTAAGCTCTTTCTGGCTCTTCCTCCATGCTGCCTTCTGCCTCATCAGTCTCGCCTTAGGATTTCGTATCTCCCGCATCTTCTTTCTGCTCCTCTTCTCAACTTCCTCCACCGTTCCGTTCAACTCCGGCGCCGGCGAAGGTGAAATCTCCGTTCCAATCCGTGTTCGTTCTTCCCTCGCTGTGAATCCGATCGCCAACGTTGAGAATCCGGTGGAAAAAGTAGCTCCGGTGAGCCGTGTGGTAGTCGGCCGGCACGGGATCCGAATCCGGCCGTGGCCGCATCCGAATCCGAATGAGGTGATGAAAGCTCATAAGATAATTGAAAGAGTTCAAACGGAGCAGAAAGCGTTGTTCGGAGTGAAGAACCCTAGGACGGTTATTGCAGTTACGCCGACGTATGTGAGGACGTTCCAGAAGCTTCACTTGATCGGTGTGATGCATTCGTTGATGCTTGTGCCGTATGATCTGGTTTGGATCGTGGTGGAAGCTGGAGGAGTGACGAATGAGACTGCTTCGATTGTTGCCAATTCAGGACTCAGAAGAATAATTCACATTGGGTTTAATCAACGGATTCCGATTTCGTGGGAGGGTAGGCATAAATTGGAAGCTCGAATGCGACTTCTTGCTTTAAGAATTGTGAGAAAAGAGAAGCTGGATGGGATTGTGATGTTTGCGGACGATAGCAATATGCATAGTATGGAGTTATTTGATGAAATTCAGAGTGTGAAATGGATAGGGGCAGTTTCGGTTGGAATACTTGTTCAGTCTGATACCGATGAATCTTCAACCTCACTGAAGGAGGAGGCACCGCCAATGCCAGTACAAGGTCCTGCTTGTAATGCGACAAATCATTTGGTTGGGTTGCATACATATGATTCCTTAAAATATGCAAGGAGAGGTGCAGTGTACATAGGTGACAGGGCACCTGTGATACCTAGGAAGCTTGAATGGTCTGGGTTTGTGTTGAACTCCAGGTTGGTTTGGAAGGATATTGATGATAAACCAGACTGGATTAAGGATATTAATGCGTTAGATGGAGTTGATGAAGATATCGATAGTCCACTGTCTCTGCTGAAAGACATCTGTGTGTTAGAACCACTTGGGAGTTGTGGACGCCAAGTTTTGCTTTGGTGGCTGCGGGTTGAAGCTCGCACAGACAGCAAATTCCCACCTCAGTGA
Protein sequence

>MsG0880045908.01.T01

MKLSTLLQNRRSNSIKEPPLFESSGDITGKSPLSSFWLFLHAAFCLISLALGFRISRIFFLLLFSTSSTVPFNSGAGEGEISVPIRVRSSLAVNPIANVENPVEKVAPVSRVVVGRHGIRIRPWPHPNPNEVMKAHKIIERVQTEQKALFGVKNPRTVIAVTPTYVRTFQKLHLIGVMHSLMLVPYDLVWIVVEAGGVTNETASIVANSGLRRIIHIGFNQRIPISWEGRHKLEARMRLLALRIVRKEKLDGIVMFADDSNMHSMELFDEIQSVKWIGAVSVGILVQSDTDESSTSLKEEAPPMPVQGPACNATNHLVGLHTYDSLKYARRGAVYIGDRAPVIPRKLEWSGFVLNSRLVWKDIDDKPDWIKDINALDGVDEDIDSPLSLLKDICVLEPLGSCGRQVLLWWLRVEARTDSKFPPQ*