Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880046147.01.T01 | XP_003608762.1 | 95.364 | 151 | 2 | 1 | 1 | 146 | 1 | 151 | 9.29E-101 | 303 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0580024979.01 | MsG0880046147.01 | 0.801708 | 7.927381e-49 | 3.811622e-46 |
| MsG0880041912.01 | MsG0880046147.01 | 0.804986 | 1.659555e-49 | 8.678738e-47 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880046147.01.T01 | MTR_4g101680 | 95.364 | 151 | 2 | 1 | 1 | 146 | 1 | 151 | 1.12e-104 | 303 |
| MsG0880046147.01.T01 | MTR_4g036030 | 82.051 | 156 | 23 | 1 | 1 | 151 | 1 | 156 | 1.45e-94 | 277 |
| MsG0880046147.01.T01 | MTR_5g012080 | 81.013 | 158 | 23 | 3 | 1 | 151 | 1 | 158 | 3.33e-92 | 271 |
| MsG0880046147.01.T01 | MTR_5g021710 | 79.592 | 147 | 25 | 1 | 5 | 146 | 6 | 152 | 3.43e-86 | 256 |
| MsG0880046147.01.T01 | MTR_8g076110 | 75.658 | 152 | 32 | 1 | 5 | 151 | 4 | 155 | 4.80e-83 | 246 |
| MsG0880046147.01.T01 | MTR_1g096430 | 71.523 | 151 | 38 | 1 | 1 | 146 | 1 | 151 | 2.44e-77 | 233 |
| MsG0880046147.01.T01 | MTR_8g024480 | 70.988 | 162 | 31 | 3 | 1 | 146 | 9 | 170 | 3.14e-75 | 231 |
| MsG0880046147.01.T01 | MTR_2g062730 | 71.918 | 146 | 35 | 2 | 7 | 146 | 10 | 155 | 2.20e-72 | 221 |
| MsG0880046147.01.T01 | MTR_4g035590 | 71.034 | 145 | 36 | 2 | 7 | 146 | 8 | 151 | 1.16e-70 | 216 |
| MsG0880046147.01.T01 | MTR_2g064090 | 58.088 | 136 | 53 | 1 | 7 | 138 | 6 | 141 | 4.95e-57 | 181 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880046147.01.T01 | AT1G12260 | 82.781 | 151 | 21 | 1 | 1 | 146 | 1 | 151 | 9.21e-93 | 272 |
| MsG0880046147.01.T01 | AT1G12260 | 82.781 | 151 | 21 | 1 | 1 | 146 | 1 | 151 | 1.41e-92 | 274 |
| MsG0880046147.01.T01 | AT1G62700 | 81.457 | 151 | 23 | 1 | 1 | 146 | 1 | 151 | 5.77e-91 | 270 |
| MsG0880046147.01.T01 | AT1G62700 | 81.457 | 151 | 23 | 1 | 1 | 146 | 1 | 151 | 5.77e-91 | 270 |
| MsG0880046147.01.T01 | AT2G18060 | 78.231 | 147 | 27 | 1 | 5 | 146 | 7 | 153 | 4.04e-84 | 251 |
| MsG0880046147.01.T01 | AT2G18060 | 78.231 | 147 | 27 | 1 | 5 | 146 | 7 | 153 | 4.04e-84 | 251 |
| MsG0880046147.01.T01 | AT4G36160 | 79.310 | 145 | 25 | 1 | 7 | 146 | 10 | 154 | 5.96e-84 | 251 |
| MsG0880046147.01.T01 | AT4G36160 | 79.310 | 145 | 25 | 1 | 7 | 146 | 10 | 154 | 5.96e-84 | 251 |
| MsG0880046147.01.T01 | AT4G36160 | 79.310 | 145 | 25 | 1 | 7 | 146 | 14 | 158 | 7.39e-84 | 251 |
| MsG0880046147.01.T01 | AT5G62380 | 76.159 | 151 | 31 | 1 | 1 | 146 | 1 | 151 | 1.86e-83 | 249 |
Find 32 sgRNAs with CRISPR-Local
Find 146 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAGATAAGAAGTATCCAAC+AGG | 0.484211 | 8:-69831246 | MsG0880046147.01.T01:CDS |
| AACTTGTTGATTACTACCTC+AGG | 0.500136 | 8:-69831655 | MsG0880046147.01.T01:CDS |
| AAGAAGGATGGGTGTATGCA+GGG | 0.571504 | 8:-69828334 | MsG0880046147.01.T01:CDS |
| AATATACTCAAAACAATTTC+TGG | 0.230974 | 8:-69831163 | MsG0880046147.01.T01:CDS |
| AATCAGATTTCTGTCCATTT+GGG | 0.310097 | 8:+69831100 | None:intergenic |
| ACGAATAGAGCAACAAAAGC+AGG | 0.489114 | 8:-69831216 | MsG0880046147.01.T01:CDS |
| ACTCAAAACAATTTCTGGTT+GGG | 0.418569 | 8:-69831158 | MsG0880046147.01.T01:CDS |
| ATACTTGTACTGCAGGAAGA+AGG | 0.571751 | 8:-69828350 | MsG0880046147.01.T01:intron |
| ATCAGATTTCTGTCCATTTG+GGG | 0.575645 | 8:+69831101 | None:intergenic |
| ATCTCTATAAAATTGAACCA+TGG | 0.552588 | 8:-69831583 | MsG0880046147.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAAATCTTATGTGATCGGTT+TGG | - | Chr8:69830524-69830543 | MsG0880046147.01.T01:intron | 30.0% | |
| AAAATGCACACATATAACTC+GGG | + | Chr8:69830485-69830504 | None:intergenic | 30.0% | |
| AACATTATCAGTTGAGTTTG+CGG | + | Chr8:69830424-69830443 | None:intergenic | 30.0% | |
| AACTATTATACTTGTACTGC+AGG | - | Chr8:69831669-69831688 | MsG0880046147.01.T01:CDS | 30.0% | |
| AATCAGATTTCTGTCCATTT+GGG | + | Chr8:69828929-69828948 | None:intergenic | 30.0% | |
| ATATGAATCAAAAGTCGTGA+CGG | + | Chr8:69829535-69829554 | None:intergenic | 30.0% | |
| ATTGCGGATATAAGTATGTA+GGG | + | Chr8:69831269-69831288 | None:intergenic | 30.0% | |
| CCATCAATACATATCTCAAA+AGG | - | Chr8:69831409-69831428 | MsG0880046147.01.T01:intron | 30.0% | |
| CGTCTTGAAACTAATCAAAA+TGG | - | Chr8:69828966-69828985 | MsG0880046147.01.T01:intron | 30.0% | |
| GAAAAAATCGGTACTAAAGA+TGG | + | Chr8:69829325-69829344 | None:intergenic | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr8 | gene | 69828316 | 69831732 | 69828316 | ID=MsG0880046147.01;Name=MsG0880046147.01 |
| Chr8 | mRNA | 69828316 | 69831732 | 69828316 | ID=MsG0880046147.01.T01;Parent=MsG0880046147.01;Name=MsG0880046147.01.T01;_AED=0.32;_eAED=0.32;_QI=0|0|0|1|1|1|3|0|152 |
| Chr8 | exon | 69831573 | 69831732 | 69831573 | ID=MsG0880046147.01.T01:exon:27301;Parent=MsG0880046147.01.T01 |
| Chr8 | exon | 69831050 | 69831306 | 69831050 | ID=MsG0880046147.01.T01:exon:27300;Parent=MsG0880046147.01.T01 |
| Chr8 | exon | 69828316 | 69828357 | 69828316 | ID=MsG0880046147.01.T01:exon:27299;Parent=MsG0880046147.01.T01 |
| Chr8 | CDS | 69831573 | 69831732 | 69831573 | ID=MsG0880046147.01.T01:cds;Parent=MsG0880046147.01.T01 |
| Chr8 | CDS | 69831050 | 69831306 | 69831050 | ID=MsG0880046147.01.T01:cds;Parent=MsG0880046147.01.T01 |
| Chr8 | CDS | 69828316 | 69828357 | 69828316 | ID=MsG0880046147.01.T01:cds;Parent=MsG0880046147.01.T01 |
| Gene Sequence |
>MsG0880046147.01.T01 ATGAATACATTTTCACATGTACCTCCAGGCTTTCGTTTTCATCCAACTGATGAAGAACTTGTTGATTACTACCTCAGGAAGAAGGTATCGTCTAAAAAGATTGATCTCGATGTCATCAAAGACGTTGATCTCTATAAAATTGAACCATGGGATCTTCAAGGAAGTGATGAACAAAATGACTGGTATTTCTTTAGTCATAAAGATAAGAAGTATCCAACAGGAACTAGAACGAATAGAGCAACAAAAGCAGGATTCTGGAAAGCCACAGGAAGAGACAAAGCAATATACTCAAAACAATTTCTGGTTGGGATGAGAAAGACTCTAGTATTTTACAAAGGACGTGCCCCAAATGGACAGAAATCTGATTGGATCATGCATGAGTATCGTCTTGAAACTAATCAAAATGGAATTTCTCAGGAAGAAGGATGGGTGTATGCAGGGTATTCAAGAAAAGAATGA |
| Protein sequence |
>MsG0880046147.01.T01 MNTFSHVPPGFRFHPTDEELVDYYLRKKVSSKKIDLDVIKDVDLYKIEPWDLQGSDEQNDWYFFSHKDKKYPTGTRTNRATKAGFWKATGRDKAIYSKQFLVGMRKTLVFYKGRAPNGQKSDWIMHEYRLETNQNGISQEEGWVYAGYSRKE* |
