Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880046216.01.T01 | XP_013457687.2 | 92.361 | 144 | 9 | 1 | 1 | 144 | 1 | 142 | 5.61E-93 | 277 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880046216.01.T01 | Q9T0F7 | 44.828 | 145 | 71 | 4 | 1 | 142 | 116 | 254 | 4.31E-30 | 113 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880046216.01.T01 | A0A072URM4 | 92.361 | 144 | 9 | 1 | 1 | 144 | 131 | 272 | 3.95e-93 | 281 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0280010788.01 | MsG0880046216.01 | 0.820439 | 6.855418e-53 | 5.424581e-50 |
| MsG0280006910.01 | MsG0880046216.01 | 0.813433 | 2.565434e-51 | 1.675163e-48 |
| MsG0280007878.01 | MsG0880046216.01 | 0.812610 | 3.888570e-51 | 2.483624e-48 |
| MsG0280008107.01 | MsG0880046216.01 | 0.804365 | 2.237100e-49 | 1.151311e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880046216.01.T01 | MTR_4g102900 | 92.361 | 144 | 9 | 1 | 1 | 144 | 131 | 272 | 1.00e-96 | 281 |
| MsG0880046216.01.T01 | MTR_8g080660 | 40.000 | 145 | 81 | 3 | 1 | 142 | 121 | 262 | 4.14e-30 | 111 |
| MsG0880046216.01.T01 | MTR_3g085270 | 39.865 | 148 | 81 | 4 | 1 | 144 | 117 | 260 | 1.17e-29 | 109 |
| MsG0880046216.01.T01 | MTR_3g005710 | 40.690 | 145 | 79 | 4 | 1 | 143 | 131 | 270 | 4.46e-29 | 108 |
| MsG0880046216.01.T01 | MTR_4g485570 | 39.189 | 148 | 81 | 4 | 1 | 144 | 124 | 266 | 1.96e-28 | 107 |
| MsG0880046216.01.T01 | MTR_4g485560 | 39.189 | 148 | 81 | 4 | 1 | 144 | 74 | 216 | 6.69e-28 | 104 |
| MsG0880046216.01.T01 | MTR_4g094895 | 38.650 | 163 | 93 | 4 | 1 | 159 | 127 | 286 | 4.24e-25 | 98.2 |
| MsG0880046216.01.T01 | MTR_7g029100 | 50.649 | 77 | 35 | 1 | 69 | 142 | 173 | 249 | 4.61e-21 | 87.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880046216.01.T01 | AT4G24910 | 52.703 | 148 | 66 | 2 | 1 | 144 | 136 | 283 | 6.12e-48 | 157 |
| MsG0880046216.01.T01 | AT1G71690 | 41.781 | 146 | 77 | 3 | 1 | 142 | 123 | 264 | 2.71e-31 | 114 |
| MsG0880046216.01.T01 | AT4G09990 | 44.828 | 145 | 71 | 4 | 1 | 142 | 116 | 254 | 4.39e-31 | 113 |
| MsG0880046216.01.T01 | AT1G09610 | 40.411 | 146 | 78 | 4 | 1 | 143 | 111 | 250 | 1.01e-29 | 110 |
| MsG0880046216.01.T01 | AT1G33800 | 43.750 | 144 | 73 | 4 | 1 | 142 | 124 | 261 | 1.72e-29 | 109 |
| MsG0880046216.01.T01 | AT2G15440 | 38.000 | 150 | 82 | 3 | 1 | 144 | 131 | 275 | 1.98e-28 | 107 |
| MsG0880046216.01.T01 | AT1G27930 | 38.621 | 145 | 83 | 3 | 1 | 142 | 115 | 256 | 3.35e-26 | 100 |
| MsG0880046216.01.T01 | AT1G67330 | 36.054 | 147 | 89 | 2 | 1 | 144 | 118 | 262 | 8.39e-26 | 99.8 |
| MsG0880046216.01.T01 | AT3G50220 | 38.816 | 152 | 78 | 4 | 1 | 144 | 141 | 285 | 4.00e-24 | 95.9 |
| MsG0880046216.01.T01 | AT5G67210 | 40.385 | 156 | 70 | 7 | 1 | 144 | 134 | 278 | 4.73e-24 | 95.9 |
Find 29 sgRNAs with CRISPR-Local
Find 33 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTTGTTACCTTTCTTGTTGT+TGG | 0.253770 | 8:-70646265 | None:intergenic |
| TTCTTCTCATATACTATTGC+AGG | 0.287959 | 8:-70646448 | None:intergenic |
| CAATAGTATATGAGAAGAAT+TGG | 0.309323 | 8:+70646452 | MsG0880046216.01.T01:CDS |
| AGGTGTCTTGAATCTGGTTC+TGG | 0.346080 | 8:-70646388 | None:intergenic |
| TAGAACACTAGCAGTGTAAA+TGG | 0.373143 | 8:-70646536 | None:intergenic |
| GTTTGAAGGTGTCTTGAATC+TGG | 0.376427 | 8:-70646394 | None:intergenic |
| TGTATTCTAAAGAGAAGTTA+TGG | 0.417535 | 8:+70646655 | MsG0880046216.01.T01:CDS |
| TCTGGTTCTGGTGCACATGC+TGG | 0.428572 | 8:-70646376 | None:intergenic |
| TGGGATGTCATAGTGGTTGA+TGG | 0.465525 | 8:+70646472 | MsG0880046216.01.T01:CDS |
| GACCGAGTCACCGGGTAGAA+TGG | 0.489909 | 8:+70646510 | MsG0880046216.01.T01:CDS |
| GTGGTTATCGTCGAGAAAGA+TGG | 0.500029 | 8:-70646236 | None:intergenic |
| AATAGTATATGAGAAGAATT+GGG | 0.500479 | 8:+70646453 | MsG0880046216.01.T01:CDS |
| TAGATCGAATGGTAGAGAAA+TGG | 0.504798 | 8:+70646599 | MsG0880046216.01.T01:CDS |
| ATCTCATCAATGAATGAAGC+AGG | 0.514333 | 8:+70646208 | None:intergenic |
| TGCCATTCTACCCGGTGACT+CGG | 0.524201 | 8:-70646512 | None:intergenic |
| TGGCGATTTGACCGAGTCAC+CGG | 0.527020 | 8:+70646501 | MsG0880046216.01.T01:CDS |
| CGACAGACCAACAACAAGAA+AGG | 0.531476 | 8:+70646258 | MsG0880046216.01.T01:CDS |
| TTCTTGTTGTTGGTCTGTCG+TGG | 0.555462 | 8:-70646255 | None:intergenic |
| GTTCTAGCTAGAGGTGGGAA+TGG | 0.584379 | 8:+70646553 | MsG0880046216.01.T01:CDS |
| TAAATGGATGCCATTCTACC+CGG | 0.598005 | 8:-70646520 | None:intergenic |
| GCTAGTGTTCTAGCTAGAGG+TGG | 0.600870 | 8:+70646547 | MsG0880046216.01.T01:CDS |
| TGTACATGATGTAGATCGAA+TGG | 0.614483 | 8:+70646588 | MsG0880046216.01.T01:CDS |
| GACTCGGTCAAATCGCCAGT+CGG | 0.616788 | 8:-70646496 | None:intergenic |
| GAAGAATTGGGATGTCATAG+TGG | 0.623144 | 8:+70646465 | MsG0880046216.01.T01:CDS |
| CTAGTGTTCTAGCTAGAGGT+GGG | 0.628712 | 8:+70646548 | MsG0880046216.01.T01:CDS |
| GGCGATTTGACCGAGTCACC+GGG | 0.635705 | 8:+70646502 | MsG0880046216.01.T01:CDS |
| ACTGCTAGTGTTCTAGCTAG+AGG | 0.636374 | 8:+70646544 | MsG0880046216.01.T01:CDS |
| ATAGTGGTTGATGGACCGAC+TGG | 0.650519 | 8:+70646481 | MsG0880046216.01.T01:CDS |
| TATCGTCGAGAAAGATGGTG+TGG | 0.672923 | 8:-70646231 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATAGTATATGAGAAGAATT+GGG | + | Chr8:70646453-70646472 | MsG0880046216.01.T01:CDS | 20.0% |
| ! | CAATAGTATATGAGAAGAAT+TGG | + | Chr8:70646452-70646471 | MsG0880046216.01.T01:CDS | 25.0% |
| !! | AGTTTATAAGCTTCTTTTGT+TGG | - | Chr8:70646334-70646353 | None:intergenic | 25.0% |
| !! | TGTATTCTAAAGAGAAGTTA+TGG | + | Chr8:70646655-70646674 | MsG0880046216.01.T01:CDS | 25.0% |
| !!! | GTTTACATTTTGATGTTTGA+AGG | - | Chr8:70646411-70646430 | None:intergenic | 25.0% |
| !!! | TTATGGCATTTTAGAATTAG+AGG | + | Chr8:70646672-70646691 | MsG0880046216.01.T01:CDS | 25.0% |
| TTCTTCTCATATACTATTGC+AGG | - | Chr8:70646451-70646470 | None:intergenic | 30.0% | |
| TAGAACACTAGCAGTGTAAA+TGG | - | Chr8:70646539-70646558 | None:intergenic | 35.0% | |
| TAGATCGAATGGTAGAGAAA+TGG | + | Chr8:70646599-70646618 | MsG0880046216.01.T01:CDS | 35.0% | |
| TGTACATGATGTAGATCGAA+TGG | + | Chr8:70646588-70646607 | MsG0880046216.01.T01:CDS | 35.0% | |
| ! | GGTAGAGAAATGGTTTTCTT+GGG | + | Chr8:70646609-70646628 | MsG0880046216.01.T01:CDS | 35.0% |
| ! | GTTGTTACCTTTCTTGTTGT+TGG | - | Chr8:70646268-70646287 | None:intergenic | 35.0% |
| ! | TGGTAGAGAAATGGTTTTCT+TGG | + | Chr8:70646608-70646627 | MsG0880046216.01.T01:CDS | 35.0% |
| GAAGAATTGGGATGTCATAG+TGG | + | Chr8:70646465-70646484 | MsG0880046216.01.T01:CDS | 40.0% | |
| TAAATGGATGCCATTCTACC+CGG | - | Chr8:70646523-70646542 | None:intergenic | 40.0% | |
| !! | GTTTGAAGGTGTCTTGAATC+TGG | - | Chr8:70646397-70646416 | None:intergenic | 40.0% |
| CGACAGACCAACAACAAGAA+AGG | + | Chr8:70646258-70646277 | MsG0880046216.01.T01:CDS | 45.0% | |
| GTGGTTATCGTCGAGAAAGA+TGG | - | Chr8:70646239-70646258 | None:intergenic | 45.0% | |
| TATCGTCGAGAAAGATGGTG+TGG | - | Chr8:70646234-70646253 | None:intergenic | 45.0% | |
| TGGGATGTCATAGTGGTTGA+TGG | + | Chr8:70646472-70646491 | MsG0880046216.01.T01:CDS | 45.0% | |
| ! | ACTGCTAGTGTTCTAGCTAG+AGG | + | Chr8:70646544-70646563 | MsG0880046216.01.T01:CDS | 45.0% |
| ! | AGGTGTCTTGAATCTGGTTC+TGG | - | Chr8:70646391-70646410 | None:intergenic | 45.0% |
| ! | CTAGTGTTCTAGCTAGAGGT+GGG | + | Chr8:70646548-70646567 | MsG0880046216.01.T01:CDS | 45.0% |
| !! | TTCTTGTTGTTGGTCTGTCG+TGG | - | Chr8:70646258-70646277 | None:intergenic | 45.0% |
| ! | GCTAGTGTTCTAGCTAGAGG+TGG | + | Chr8:70646547-70646566 | MsG0880046216.01.T01:CDS | 50.0% |
| ! | GTTCTAGCTAGAGGTGGGAA+TGG | + | Chr8:70646553-70646572 | MsG0880046216.01.T01:CDS | 50.0% |
| !! | ATAGTGGTTGATGGACCGAC+TGG | + | Chr8:70646481-70646500 | MsG0880046216.01.T01:CDS | 50.0% |
| TGCCATTCTACCCGGTGACT+CGG | - | Chr8:70646515-70646534 | None:intergenic | 55.0% | |
| TGGCGATTTGACCGAGTCAC+CGG | + | Chr8:70646501-70646520 | MsG0880046216.01.T01:CDS | 55.0% | |
| ! | GACTCGGTCAAATCGCCAGT+CGG | - | Chr8:70646499-70646518 | None:intergenic | 55.0% |
| !! | TCTGGTTCTGGTGCACATGC+TGG | - | Chr8:70646379-70646398 | None:intergenic | 55.0% |
| GACCGAGTCACCGGGTAGAA+TGG | + | Chr8:70646510-70646529 | MsG0880046216.01.T01:CDS | 60.0% | |
| GGCGATTTGACCGAGTCACC+GGG | + | Chr8:70646502-70646521 | MsG0880046216.01.T01:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr8 | gene | 70646217 | 70646702 | 70646217 | ID=MsG0880046216.01;Name=MsG0880046216.01 |
| Chr8 | mRNA | 70646217 | 70646702 | 70646217 | ID=MsG0880046216.01.T01;Parent=MsG0880046216.01;Name=MsG0880046216.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|161 |
| Chr8 | exon | 70646217 | 70646702 | 70646217 | ID=MsG0880046216.01.T01:exon:27666;Parent=MsG0880046216.01.T01 |
| Chr8 | CDS | 70646217 | 70646702 | 70646217 | ID=MsG0880046216.01.T01:cds;Parent=MsG0880046216.01.T01 |
| Gene Sequence |
| Protein sequence |
