AlfalfaGEDB | MsG0280006910.01

M. sativa cultivar ZhongmuNo.1 / MsG0280006910.01

KEGG: -
GO: -
PFAM: PF10250

Nr:

Query id	Subject id	identity %	alignment length	mis matches	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280006910.01.T01	KEH36621.1	95.745	188	8	44	231	122	309	1.16E-121	372
MsG0280006910.01.T01	KEH36621.1	97.674	43	1	1	43	1	43	8.59E-18	95.5
MsG0280006910.01.T01	KEH36621.1	93.023	43	3	247	289	435	477	5.72E-15	87.4

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280006910.01.T01	F4IAL1	43.841	276	120	8	32	284	101	364	1.33E-58	200

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280006910.01.T01	A0A072V4M7	95.745	188	8	0	44	231	122	309	5.56e-122	372

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0180000334.01	MsG0280006910.01	0.808846	2.533897e-50	1.464344e-47
MsG0180001553.01	MsG0280006910.01	0.826440	2.709077e-54	2.540851e-51
MsG0180001892.01	MsG0280006910.01	0.808721	2.694237e-50	1.551844e-47
MsG0180003455.01	MsG0280006910.01	0.824912	6.240203e-54	5.603696e-51
MsG0180003797.01	MsG0280006910.01	0.807148	5.822232e-50	3.218499e-47
MsG0180004099.01	MsG0280006910.01	0.809511	1.825217e-50	1.073702e-47
MsG0180004389.01	MsG0280006910.01	0.818656	1.749337e-52	1.317267e-49
MsG0180004802.01	MsG0280006910.01	0.819369	1.204231e-52	9.248443e-50
MsG0180006024.01	MsG0280006910.01	0.828912	6.904698e-55	6.957862e-52
MsG0280006299.01	MsG0280006910.01	0.813544	2.426355e-51	1.589115e-48
MsG0280006567.01	MsG0280006910.01	0.816551	5.218212e-52	3.708786e-49
MsG0280006910.01	MsG0280007878.01	0.855307	6.887741e-62	1.583403e-58
MsG0280006910.01	MsG0280008106.01	0.840967	6.318717e-58	9.149272e-55
MsG0280006910.01	MsG0280008107.01	0.838400	2.940770e-57	3.934311e-54
MsG0280006910.01	MsG0280008615.01	0.820817	5.613390e-53	4.488600e-50
MsG0280006910.01	MsG0280008909.01	0.800575	1.351264e-48	6.313859e-46
MsG0280006910.01	MsG0280009421.01	0.812738	3.644306e-51	2.335565e-48
MsG0280006910.01	MsG0280010788.01	0.878851	2.009593e-69	1.065345e-65
MsG0280006910.01	MsG0280010907.01	0.800864	1.179952e-48	5.554017e-46
MsG0280006910.01	MsG0280011101.01	0.800104	1.685299e-48	7.780987e-46
MsG0280006910.01	MsG0280011305.01	0.812614	3.880554e-51	2.478793e-48
MsG0280006910.01	MsG0280011329.01	0.825621	4.241056e-54	3.885927e-51
MsG0280006910.01	MsG0380011648.01	0.807450	5.023880e-50	2.799230e-47
MsG0280006910.01	MsG0380013176.01	0.809387	1.940890e-50	1.138043e-47
MsG0280006910.01	MsG0380013549.01	0.810065	1.386999e-50	8.279524e-48
MsG0280006910.01	MsG0380014498.01	0.816682	4.874886e-52	3.477314e-49
MsG0280006910.01	MsG0380014537.01	0.821687	3.537318e-53	2.898045e-50
MsG0280006910.01	MsG0380015196.01	0.835908	1.275636e-56	1.582065e-53
MsG0280006910.01	MsG0380015332.01	0.808239	3.414363e-50	1.942161e-47
MsG0280006910.01	MsG0380015631.01	0.818293	2.113646e-52	1.575817e-49
MsG0280006910.01	MsG0480019350.01	0.802658	5.054667e-49	2.489854e-46
MsG0280006910.01	MsG0480021056.01	0.863789	1.936570e-64	5.944336e-61
MsG0280006910.01	MsG0480021332.01	0.827915	1.201442e-54	1.176104e-51
MsG0280006910.01	MsG0480021923.01	0.801779	7.665153e-49	3.692207e-46
MsG0280006910.01	MsG0480022054.01	0.846632	1.922362e-59	3.329744e-56
MsG0280006910.01	MsG0480022322.01	0.810018	1.419950e-50	8.466101e-48
MsG0280006910.01	MsG0480023463.01	0.803895	2.801900e-49	1.424747e-46
MsG0280006910.01	MsG0480023489.01	0.821503	3.901059e-53	3.179318e-50
MsG0280006910.01	MsG0580026086.01	0.817551	3.110419e-52	2.272162e-49
MsG0280006910.01	MsG0580026309.01	0.807990	3.859176e-50	2.180694e-47
MsG0280006910.01	MsG0680030447.01	0.854214	1.428953e-61	3.168320e-58
MsG0280006910.01	MsG0680030882.01	0.806046	9.949270e-50	5.346496e-47
MsG0280006910.01	MsG0680031617.01	0.832751	7.905876e-56	8.919337e-53
MsG0280006910.01	MsG0680035692.01	0.800792	1.220484e-48	5.733998e-46
MsG0280006910.01	MsG0780037054.01	0.815956	7.085207e-52	4.954687e-49
MsG0280006910.01	MsG0780038377.01	0.807374	5.214554e-50	2.899565e-47
MsG0280006910.01	MsG0780038439.01	0.814972	1.173253e-51	7.986673e-49
MsG0280006910.01	MsG0780038691.01	0.814652	1.381793e-51	9.325339e-49
MsG0280006910.01	MsG0780039170.01	0.813053	3.108801e-51	2.008996e-48
MsG0280006910.01	MsG0780039257.01	0.847812	9.125190e-60	1.641378e-56
MsG0280006910.01	MsG0780039443.01	0.800209	1.604247e-48	7.426489e-46
MsG0280006910.01	MsG0880042520.01	0.810689	1.017535e-50	6.174849e-48
MsG0280006910.01	MsG0880044457.01	0.824424	8.133859e-54	7.201275e-51
MsG0280006910.01	MsG0880045111.01	0.819688	1.018357e-52	7.891289e-50
MsG0280006910.01	MsG0880045164.01	0.808396	3.162025e-50	1.806032e-47
MsG0280006910.01	MsG0880045607.01	0.819477	1.137634e-52	8.764376e-50
MsG0280006910.01	MsG0880046004.01	0.803410	3.533101e-49	1.774146e-46
MsG0280006910.01	MsG0880046216.01	0.813433	2.565434e-51	1.675163e-48

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280006910.01.T01	MTR_2g016470	95.745	188	8	0	44	231	122	309	1.41e-125	372
MsG0280006910.01.T01	MTR_2g016470	97.674	43	1	0	1	43	1	43	1.04e-21	95.5
MsG0280006910.01.T01	MTR_2g016470	93.023	43	3	0	247	289	435	477	6.93e-19	87.4
MsG0280006910.01.T01	MTR_4g132590	53.081	211	77	6	74	274	31	229	5.58e-53	181
MsG0280006910.01.T01	MTR_4g132590	53.081	211	77	6	74	274	130	328	2.57e-52	181
MsG0280006910.01.T01	MTR_1g031590	46.711	152	69	4	80	228	123	265	1.05e-39	146
MsG0280006910.01.T01	MTR_4g084570	46.711	152	69	4	80	228	124	266	3.19e-39	145
MsG0280006910.01.T01	MTR_3g086570	41.667	156	85	3	77	228	114	267	4.02e-34	129
MsG0280006910.01.T01	MTR_3g086570	41.667	156	85	3	77	228	114	267	1.58e-33	129
MsG0280006910.01.T01	MTR_3g005340	46.622	148	74	3	81	228	121	263	7.64e-33	127
MsG0280006910.01.T01	MTR_5g059260	47.059	136	70	2	94	228	139	273	3.49e-31	122
MsG0280006910.01.T01	MTR_5g032100	42.424	165	88	3	61	222	40	200	7.88e-29	115
MsG0280006910.01.T01	MTR_3g100310	42.012	169	83	5	61	222	54	214	2.91e-28	114
MsG0280006910.01.T01	MTR_5g064620	41.727	139	75	3	50	188	56	188	1.33e-25	106
MsG0280006910.01.T01	MTR_4g100810	36.242	149	86	4	82	228	80	221	3.57e-25	105
MsG0280006910.01.T01	MTR_6g016015	40.458	131	75	1	100	230	104	231	5.19e-25	105
MsG0280006910.01.T01	MTR_4g110030	40.476	126	72	2	105	230	110	232	1.10e-24	103
MsG0280006910.01.T01	MTR_2g059350	38.168	131	68	2	105	230	109	231	4.62e-24	102
MsG0280006910.01.T01	MTR_4g019210	37.333	150	84	4	81	230	205	344	1.21e-23	101
MsG0280006910.01.T01	MTR_5g042200	38.095	126	74	1	105	230	79	200	4.27e-23	99.4
MsG0280006910.01.T01	MTR_4g125460	41.935	124	59	2	110	230	88	201	7.85e-23	98.6
MsG0280006910.01.T01	MTR_4g125460	41.935	124	59	2	110	230	227	340	1.18e-22	98.6
MsG0280006910.01.T01	MTR_3g099480	46.296	108	54	2	81	188	114	217	1.97e-22	97.8
MsG0280006910.01.T01	MTR_1g102030	27.931	290	126	8	81	290	61	347	3.91e-22	96.7
MsG0280006910.01.T01	MTR_4g082310	37.302	126	75	1	105	230	91	212	8.98e-22	95.5
MsG0280006910.01.T01	MTR_1g101985	43.810	105	56	2	81	185	61	162	1.43e-21	90.1
MsG0280006910.01.T01	MTR_5g099010	32.124	193	109	6	50	241	72	243	1.60e-21	95.1
MsG0280006910.01.T01	MTR_3g115220	39.370	127	68	2	104	228	104	223	3.32e-21	94.0
MsG0280006910.01.T01	MTR_4g010990	31.429	175	94	2	93	267	57	205	4.66e-21	93.2
MsG0280006910.01.T01	MTR_5g012770	42.157	102	58	1	88	188	100	201	4.77e-21	93.6
MsG0280006910.01.T01	MTR_3g096020	39.474	114	65	2	77	190	159	268	2.90e-20	91.3
MsG0280006910.01.T01	MTR_5g081690	35.714	140	76	3	106	238	109	241	1.42e-19	89.4
MsG0280006910.01.T01	MTR_4g023030	35.593	118	71	2	72	186	193	308	1.94e-16	80.1
MsG0280006910.01.T01	MTR_3g026650	36.283	113	63	3	79	186	140	248	3.77e-16	78.6
MsG0280006910.01.T01	MTR_3g026650	36.283	113	63	3	79	186	140	248	5.86e-16	78.6
MsG0280006910.01.T01	MTR_3g026650	36.283	113	63	3	79	186	140	248	7.63e-16	78.2
MsG0280006910.01.T01	MTR_4g010990	29.787	141	73	2	127	267	2	116	2.97e-13	69.7
MsG0280006910.01.T01	MTR_7g017330	27.184	206	126	6	81	281	70	256	3.17e-12	67.4

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280006910.01.T01	AT1G11990	47.036	253	110	7	32	274	101	339	1.15e-60	203
MsG0280006910.01.T01	AT1G11990	43.841	276	120	8	32	284	101	364	1.36e-59	200
MsG0280006910.01.T01	AT1G62330	56.863	204	74	5	59	260	172	363	4.73e-58	197
MsG0280006910.01.T01	AT1G29200	61.111	180	67	3	54	231	11	189	7.63e-58	193
MsG0280006910.01.T01	AT1G29200	75.610	41	10	0	249	289	316	356	4.01e-13	70.1
MsG0280006910.01.T01	AT1G29200	61.111	180	67	3	54	231	11	189	7.63e-58	193
MsG0280006910.01.T01	AT1G29200	75.610	41	10	0	249	289	316	356	4.01e-13	70.1
MsG0280006910.01.T01	AT1G29200	61.111	180	67	3	54	231	127	305	5.06e-57	194
MsG0280006910.01.T01	AT1G29200	75.610	41	10	0	249	289	432	472	3.91e-13	70.1
MsG0280006910.01.T01	AT1G29200	68.103	116	36	1	116	231	1	115	3.27e-39	142
MsG0280006910.01.T01	AT1G29200	75.610	41	10	0	249	289	242	282	4.11e-13	69.7
MsG0280006910.01.T01	AT1G14970	45.333	150	74	4	80	228	9	151	1.72e-34	130
MsG0280006910.01.T01	AT1G14970	45.333	150	74	4	80	228	129	271	5.92e-34	130
MsG0280006910.01.T01	AT2G01480	42.667	150	78	4	80	228	128	270	2.51e-32	125
MsG0280006910.01.T01	AT2G01480	42.667	150	78	4	80	228	128	270	2.70e-32	125
MsG0280006910.01.T01	AT3G26370	39.744	156	88	3	77	228	122	275	3.68e-32	123
MsG0280006910.01.T01	AT1G35510	38.947	190	98	5	72	249	121	304	5.41e-32	125
MsG0280006910.01.T01	AT3G26370	39.744	156	88	3	77	228	122	275	2.16e-31	123
MsG0280006910.01.T01	AT1G14970	48.739	119	58	2	111	228	9	125	1.44e-30	119
MsG0280006910.01.T01	AT1G38065	45.390	141	70	2	94	228	3	142	1.46e-29	117
MsG0280006910.01.T01	AT1G38131	42.675	157	82	3	78	228	117	271	3.04e-29	117
MsG0280006910.01.T01	AT1G38065	42.384	151	70	2	94	228	3	152	7.05e-28	112
MsG0280006910.01.T01	AT5G65470	41.212	165	88	4	61	222	41	199	2.58e-27	111
MsG0280006910.01.T01	AT4G24530	38.922	167	91	5	61	222	55	215	6.17e-26	107
MsG0280006910.01.T01	AT1G04910	28.664	307	144	9	64	298	44	347	1.50e-25	106
MsG0280006910.01.T01	AT2G37980	38.000	150	83	4	81	230	199	338	4.73e-25	105
MsG0280006910.01.T01	AT5G01100	43.243	111	59	2	81	191	190	296	5.72e-25	105
MsG0280006910.01.T01	AT1G14020	41.129	124	69	1	107	230	87	206	2.78e-24	102
MsG0280006910.01.T01	AT5G35570	34.222	225	124	8	14	231	156	363	3.02e-24	103
MsG0280006910.01.T01	AT1G52630	38.065	155	81	4	79	230	44	186	4.02e-24	102
MsG0280006910.01.T01	AT5G15740	35.878	131	81	1	100	230	95	222	1.25e-23	100
MsG0280006910.01.T01	AT1G22460	43.363	113	56	3	81	191	134	240	2.22e-23	100
MsG0280006910.01.T01	AT3G02250	36.641	131	80	1	100	230	95	222	2.77e-23	100
MsG0280006910.01.T01	AT4G38390	36.842	152	87	4	81	230	94	238	2.80e-23	100
MsG0280006910.01.T01	AT3G54100	35.333	150	87	4	81	230	195	334	2.90e-22	97.4
MsG0280006910.01.T01	AT5G64600	43.103	116	58	3	78	191	88	197	3.21e-22	97.1
MsG0280006910.01.T01	AT2G03280	32.895	152	89	2	107	249	84	231	4.04e-21	93.6
MsG0280006910.01.T01	AT4G16650	30.601	183	112	4	76	249	88	264	1.03e-18	86.7
MsG0280006910.01.T01	AT4G16650	30.601	183	112	4	76	249	88	264	1.03e-18	86.7
MsG0280006910.01.T01	AT1G76270	32.895	152	88	4	110	253	105	250	7.00e-18	84.3
MsG0280006910.01.T01	AT5G63390	29.375	160	108	2	30	186	82	239	8.82e-17	80.9
MsG0280006910.01.T01	AT1G20550	30.968	155	87	4	110	253	108	253	1.24e-16	80.5
MsG0280006910.01.T01	AT2G03280	27.933	179	89	3	107	249	84	258	3.99e-16	79.0
MsG0280006910.01.T01	AT2G44500	37.963	108	62	2	82	186	147	252	1.00e-15	77.4
MsG0280006910.01.T01	AT2G44500	46.575	73	39	0	114	186	180	252	1.16e-15	77.8
MsG0280006910.01.T01	AT1G52630	37.500	104	58	1	127	230	2	98	6.95e-15	74.7
MsG0280006910.01.T01	AT3G07900	34.259	108	66	2	82	186	158	263	4.60e-14	72.8
MsG0280006910.01.T01	AT3G30300	28.780	205	120	7	81	279	72	256	6.84e-14	72.4
MsG0280006910.01.T01	AT3G30300	28.780	205	120	7	81	279	72	256	6.84e-14	72.4
MsG0280006910.01.T01	AT3G03810	32.370	173	106	5	60	228	42	207	2.19e-13	70.9
MsG0280006910.01.T01	AT3G03810	32.370	173	106	5	60	228	42	207	2.19e-13	70.9

Find 103 sgRNAs with CRISPR-Local

Find 218 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CCACTGAACTTGCACCTTTC+AGG	0.144294	2:-7858910	MsG0280006910.01.T01:three_prime_UTR
ACATTGCTGTAAAGAAATTT+TGG	0.146814	2:+7860491	None:intergenic
AAGACCAACTCCTGAATTTA+AGG	0.157623	2:-7861863	MsG0280006910.01.T01:CDS
CATAAAGATGCCTGCTTATA+TGG	0.167660	2:+7861131	None:intergenic
CAACTTTCATCATTGGTATC+TGG	0.171938	2:-7858397	MsG0280006910.01.T01:three_prime_UTR
CTCCAATGGATTTGAGAAAA+AGG	0.174801	2:-7859104	MsG0280006910.01.T01:CDS
ATATGGTTCCGTCCATAATT+TGG	0.206709	2:+7861148	None:intergenic
CATATAAGCAGGCATCTTTA+TGG	0.209772	2:-7861130	MsG0280006910.01.T01:CDS
TCCTATGCCTTCTGATATTC+AGG	0.236744	2:-7859988	MsG0280006910.01.T01:intron
AATTCAGGAGTTGGTCTTCT+TGG	0.244745	2:+7861868	None:intergenic
CTTGGCTAGTTCTCGTATTA+TGG	0.246673	2:-7859004	MsG0280006910.01.T01:CDS
AGCAAGAGTCATCCAAATTA+TGG	0.280319	2:-7861160	MsG0280006910.01.T01:CDS
CTTATACATAAACACTTAAA+TGG	0.305607	2:+7858017	None:intergenic
AGAACAAGTGCTGCTTCTTC+TGG	0.322296	2:+7859063	None:intergenic
TGTTGTTTGCACATGCATTC+TGG	0.323302	2:+7858181	None:intergenic
AAATAGAATTGTTCACTTCC+TGG	0.325555	2:-7860035	MsG0280006910.01.T01:CDS
GAATAACAAGAGTTGCATTC+AGG	0.325705	2:+7860513	None:intergenic
TTGAATGCAGATGGCTGCTT+TGG	0.348889	2:-7858473	MsG0280006910.01.T01:intron
GCACTTGTTCTAGCAGGTCT+TGG	0.391956	2:-7859051	MsG0280006910.01.T01:CDS
AACTTTCATCATTGGTATCT+GGG	0.393910	2:-7858396	MsG0280006910.01.T01:three_prime_UTR
ACAAGAAACTTACATTTACT+TGG	0.395071	2:-7859022	MsG0280006910.01.T01:CDS
ATGCATGTGCAAACAACATT+AGG	0.400899	2:-7858176	MsG0280006910.01.T01:three_prime_UTR
CTAGCAGCACCCTTAAATTC+AGG	0.409298	2:+7861853	None:intergenic
CCCGTCTGAAATAAAATCTC+TGG	0.415268	2:-7860246	MsG0280006910.01.T01:CDS
GTACGATCGGCTTCTAAACT+TGG	0.417488	2:-7861327	MsG0280006910.01.T01:CDS
AAAAGTTTATATACCTGGAT+TGG	0.424503	2:+7861079	None:intergenic
CCAGTTTGGTGACATATATC+AGG	0.426949	2:-7860321	MsG0280006910.01.T01:CDS
AATTTCTTTACAGCAATGTA+TGG	0.429014	2:-7860487	MsG0280006910.01.T01:CDS
GGTGCTGCTAGAGGAATGTT+AGG	0.433994	2:-7861841	MsG0280006910.01.T01:CDS
TCTGGGTTCAGAACCTACTA+TGG	0.437017	2:-7858379	MsG0280006910.01.T01:three_prime_UTR
TGCAGAAGCAATTGGTAGCC+AGG	0.449547	2:-7860222	MsG0280006910.01.T01:intron
AATATTCTGAAGGACGATAT+CGG	0.450509	2:-7860284	MsG0280006910.01.T01:CDS
TGGTAGCCAACTTTCATCAT+TGG	0.450993	2:-7858404	MsG0280006910.01.T01:three_prime_UTR
CAAGTTCAGTGGTGGTAAGA+AGG	0.463528	2:+7858921	None:intergenic
GCGGCTAGCCTTGTCTTGTT+CGG	0.464204	2:+7858331	None:intergenic
CCTGGATATGCAAATCGACT+TGG	0.465692	2:-7860017	MsG0280006910.01.T01:CDS
CCTGATATATGTCACCAAAC+TGG	0.467370	2:+7860321	None:intergenic
GCTGCTTCTTCTGGTGTCAA+TGG	0.469728	2:+7859072	None:intergenic
TTATAGCTCTATTTCTCCAA+TGG	0.472089	2:-7859118	MsG0280006910.01.T01:intron
TTCAAAACCATTCCATCCTA+TGG	0.473494	2:+7858288	None:intergenic
AGACCAACTCCTGAATTTAA+GGG	0.477088	2:-7861862	MsG0280006910.01.T01:CDS
CGATTGTTTCTCAGTGATGT+AGG	0.477666	2:+7861361	None:intergenic
TACATCTTAGTCAGTGCAAA+TGG	0.478144	2:-7860765	MsG0280006910.01.T01:CDS
AATGCTGGCATAAGGAGTTA+TGG	0.478737	2:-7858232	MsG0280006910.01.T01:three_prime_UTR
CAGATACCAATGATGAAAGT+TGG	0.480381	2:+7858398	None:intergenic
CCAAGTCGATTTGCATATCC+AGG	0.484649	2:+7860017	None:intergenic
ATGATCCACCGTCGTGGAAG+AGG	0.489666	2:-7861789	MsG0280006910.01.T01:CDS
ATTTAAGTGCCTATAGCATA+AGG	0.493481	2:-7858045	MsG0280006910.01.T01:three_prime_UTR
CCTTGTCTTGTTCGGCCGCA+AGG	0.502900	2:+7858339	None:intergenic
GAAGCAGCACTTGTTCTAGC+AGG	0.503559	2:-7859057	MsG0280006910.01.T01:CDS
GCACCCTTAAATTCAGGAGT+TGG	0.505405	2:+7861859	None:intergenic
AAGGTCAGAATGCTGGCATA+AGG	0.510386	2:-7858240	MsG0280006910.01.T01:three_prime_UTR
ATGGTAAGACCTTATGCTAT+AGG	0.514469	2:+7858036	None:intergenic
TCATACCTCTGCAAGAGCAC+TGG	0.517203	2:+7861301	None:intergenic
AGAAAAGTTCAAACCAATCC+AGG	0.526293	2:-7861092	MsG0280006910.01.T01:intron
AGCCGATCGTACATATGTAC+AGG	0.532348	2:+7861338	None:intergenic
TCTCTGGATGCAGAAGCAAT+TGG	0.533798	2:-7860230	MsG0280006910.01.T01:CDS
GTCTTGTTCGGCCGCAAGGT+AGG	0.536587	2:+7858343	None:intergenic
TCTTTACAGCAATGTATGGA+AGG	0.538127	2:-7860483	MsG0280006910.01.T01:CDS
TGGTCAAGTTTGTTAATGCA+AGG	0.542542	2:+7858116	None:intergenic
GAAGCATATATAGATATCCT+AGG	0.543699	2:-7858207	MsG0280006910.01.T01:three_prime_UTR
GGGAAATCTAAGAAAAGTAA+TGG	0.547296	2:-7860789	MsG0280006910.01.T01:CDS
ATTGGGATAGAGTCTATTGA+AGG	0.548098	2:+7858961	None:intergenic
AGCATGACCGCCGCCATAGT+AGG	0.548625	2:+7858366	None:intergenic
GATAGTATAATGGGAAAACA+AGG	0.553391	2:-7861895	None:intergenic
GAATTTAAGGGTGCTGCTAG+AGG	0.557595	2:-7861850	MsG0280006910.01.T01:CDS
TTATGGAGGAAACTCAAGAA+TGG	0.560261	2:-7858987	MsG0280006910.01.T01:CDS
ATTCTGGAAGGTCAGAATGC+TGG	0.561057	2:-7858247	MsG0280006910.01.T01:three_prime_UTR
AGATGCAGTTCTTGCAAAGG+AGG	0.569131	2:-7860104	MsG0280006910.01.T01:CDS
TACAGATGCAGTTCTTGCAA+AGG	0.569727	2:-7860107	MsG0280006910.01.T01:CDS
AATGACACCATAGGATGGAA+TGG	0.570826	2:-7858295	MsG0280006910.01.T01:three_prime_UTR
ATGGACGGAACCATATAAGC+AGG	0.571410	2:-7861141	MsG0280006910.01.T01:CDS
CGCCTGTACATATGTACGAT+CGG	0.573912	2:-7861340	MsG0280006910.01.T01:CDS
TTCAGAACCTACTATGGCGG+CGG	0.576194	2:-7858373	MsG0280006910.01.T01:three_prime_UTR
GATTTGCAATGCTGTGGCTG+TGG	0.576778	2:-7860543	MsG0280006910.01.T01:CDS
TTTGCTGAGAATGACACCAT+AGG	0.579883	2:-7858304	MsG0280006910.01.T01:three_prime_UTR
CTGAGAATGACACCATAGGA+TGG	0.581020	2:-7858300	MsG0280006910.01.T01:three_prime_UTR
GTCATCCTCTTCCACGACGG+TGG	0.586872	2:+7861784	None:intergenic
GGCGTCCAGTGCTCTTGCAG+AGG	0.591953	2:-7861306	MsG0280006910.01.T01:intron
GCAATGTATGGAAGGATCCA+AGG	0.593126	2:-7860475	MsG0280006910.01.T01:intron
CCTTGCGGCCGAACAAGACA+AGG	0.594618	2:-7858339	MsG0280006910.01.T01:three_prime_UTR
GGCTAGTTCTCGTATTATGG+AGG	0.602168	2:-7859001	MsG0280006910.01.T01:CDS
GTATTTCATAAATATTCTGA+AGG	0.610405	2:-7860294	MsG0280006910.01.T01:CDS
GGACGATATCGGTATTGTGA+AGG	0.620369	2:-7860273	MsG0280006910.01.T01:CDS
CTTGTGACCTGAATATCAGA+AGG	0.622679	2:+7859981	None:intergenic
CCTGAAAGGTGCAAGTTCAG+TGG	0.624956	2:+7858910	None:intergenic
CACATGCATTCTGGACACCT+AGG	0.625165	2:+7858190	None:intergenic
TTGGCTACCAGAGTCAGTCA+TGG	0.632066	2:+7858417	None:intergenic
ATCTTAGTCAGTGCAAATGG+TGG	0.642391	2:-7860762	MsG0280006910.01.T01:CDS
ACCTGAATATCAGAAGGCAT+AGG	0.644694	2:+7859987	None:intergenic
AGAGTCATCCAAATTATGGA+CGG	0.649877	2:-7861156	MsG0280006910.01.T01:CDS
CTATCCCAATGTAGTCACAA+AGG	0.650613	2:-7858948	MsG0280006910.01.T01:exon
TTTGATATAGTCAGCAACGG+TGG	0.656737	2:+7860081	None:intergenic
GAAAGGTGCAAGTTCAGTGG+TGG	0.661184	2:+7858913	None:intergenic
TGGAGGAAACTCAAGAATGG+AGG	0.664757	2:-7858984	MsG0280006910.01.T01:CDS
CAAGCAATGATCCACCGTCG+TGG	0.665566	2:-7861795	MsG0280006910.01.T01:CDS
ATAGTATAATGGGAAAACAA+GGG	0.688885	2:-7861894	None:intergenic
GCTGTCATCCTCTTCCACGA+CGG	0.692206	2:+7861781	None:intergenic
AATACAGATTTGCAATGCTG+TGG	0.698209	2:-7860549	MsG0280006910.01.T01:intron
GGGTTCAGAACCTACTATGG+CGG	0.710382	2:-7858376	MsG0280006910.01.T01:three_prime_UTR
TGTGTGATTTATGAACAAAG+TGG	0.714704	2:+7858096	None:intergenic
GCGGTCATGCTCCTACCTTG+CGG	0.733987	2:-7858354	MsG0280006910.01.T01:three_prime_UTR
GTCGTGGAAGAGGATGACAG+CGG	0.806642	2:-7861779	MsG0280006910.01.T01:intron

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	ATAATACTATTATGTAAAAT+AGG	-	Chr2:7858903-7858922	MsG0280006910.01.T01:three_prime_UTR	10.0%
!!!	ATTAATTTTTCATTATCACA+TGG	-	Chr2:7859433-7859452	MsG0280006910.01.T01:intron	15.0%
!!	AAAATACTGAGCTTAAATTA+TGG	-	Chr2:7861164-7861183	MsG0280006910.01.T01:CDS	20.0%
!!	AGTAATATGGAAATTAATCA+AGG	+	Chr2:7861326-7861345	None:intergenic	20.0%
!!	ATAAGAAAAGTTTATATACC+TGG	+	Chr2:7858789-7858808	None:intergenic	20.0%
!!	ATATTAAACATAGTATACCT+TGG	+	Chr2:7859405-7859424	None:intergenic	20.0%
!!	ATTGTTAAATTAGGATCAAA+CGG	+	Chr2:7861191-7861210	None:intergenic	20.0%
!!	CTTATACATAAACACTTAAA+TGG	+	Chr2:7861846-7861865	None:intergenic	20.0%
!!	CTTTATGAAATTTCCAATAA+TGG	-	Chr2:7859266-7859285	MsG0280006910.01.T01:intron	20.0%
!!	GCAAAAATCAATGAAAAATA+TGG	+	Chr2:7858427-7858446	None:intergenic	20.0%
!!	GTAATATGGAAATTAATCAA+GGG	+	Chr2:7861325-7861344	None:intergenic	20.0%
!!	GTATTTCATAAATATTCTGA+AGG	-	Chr2:7859566-7859585	MsG0280006910.01.T01:intron	20.0%
!!	TCAAAGTACAATACTATTAA+GGG	-	Chr2:7858160-7858179	MsG0280006910.01.T01:three_prime_UTR	20.0%
!!	TTACATCATTTCTCATATTA+AGG	-	Chr2:7859162-7859181	MsG0280006910.01.T01:intron	20.0%
!!	TTCAAAGTACAATACTATTA+AGG	-	Chr2:7858159-7858178	MsG0280006910.01.T01:three_prime_UTR	20.0%
!!!	ATTCTATTTTTCAAAAGAAG+AGG	+	Chr2:7859814-7859833	None:intergenic	20.0%
!!!	CTTAAGATTTGAAATAGATA+TGG	-	Chr2:7860507-7860526	MsG0280006910.01.T01:CDS	20.0%
!!!	TAACATACTTTTTAATGAGA+AGG	-	Chr2:7858467-7858486	MsG0280006910.01.T01:intron	20.0%
!!!	TTTCATTGATTTTTGCAAAT+TGG	-	Chr2:7858431-7858450	MsG0280006910.01.T01:three_prime_UTR	20.0%
!!!	TTTTGTTGTTGATATTTATG+TGG	-	Chr2:7860702-7860721	MsG0280006910.01.T01:intron	20.0%
!	AAAAGTTTATATACCTGGAT+TGG	+	Chr2:7858784-7858803	None:intergenic	25.0%
!	AATTTCTTTACAGCAATGTA+TGG	-	Chr2:7859373-7859392	MsG0280006910.01.T01:intron	25.0%
!	ACAAGAAACTTACATTTACT+TGG	-	Chr2:7860838-7860857	MsG0280006910.01.T01:intron	25.0%
!	ACCTATAGCTTACAATAATA+TGG	+	Chr2:7858351-7858370	None:intergenic	25.0%
!	AGATGAAAATAGAATCAAGA+AGG	+	Chr2:7858252-7858271	None:intergenic	25.0%
!	ATAGTATTTAGTGTACTGTA+AGG	-	Chr2:7860224-7860243	MsG0280006910.01.T01:CDS	25.0%
!	ATTCTCATTTGCATCTTAAA+AGG	-	Chr2:7861018-7861037	MsG0280006910.01.T01:intron	25.0%
!	CAACATATTTGTATATGTTG+CGG	+	Chr2:7861253-7861272	None:intergenic	25.0%
!	CCAAAGGATTAGAAAATATA+GGG	-	Chr2:7860385-7860404	MsG0280006910.01.T01:intron	25.0%
!	TATCAACAACAAAATTAACG+AGG	+	Chr2:7860696-7860715	None:intergenic	25.0%
!	TATTCAAAACTAGACAAATG+AGG	+	Chr2:7859909-7859928	None:intergenic	25.0%
!	TCCAAAGGATTAGAAAATAT+AGG	-	Chr2:7860384-7860403	MsG0280006910.01.T01:intron	25.0%
!	TCCATATTATTGTAAGCTAT+AGG	-	Chr2:7858347-7858366	MsG0280006910.01.T01:three_prime_UTR	25.0%
!	TTATACAGTCAAGATCATAT+AGG	+	Chr2:7859243-7859262	None:intergenic	25.0%
!	TTCTCATTTGCATCTTAAAA+GGG	-	Chr2:7861019-7861038	MsG0280006910.01.T01:intron	25.0%
!	TTGTTATCAAGTACATTGAT+TGG	+	Chr2:7859681-7859700	None:intergenic	25.0%
!!	AAGATTTGAAATAGATATGG+TGG	-	Chr2:7860510-7860529	MsG0280006910.01.T01:CDS	25.0%
!!	ACATTGCTGTAAAGAAATTT+TGG	+	Chr2:7859372-7859391	None:intergenic	25.0%
!!	ACTATTAAGGGATTTTATGT+TGG	-	Chr2:7858172-7858191	MsG0280006910.01.T01:three_prime_UTR	25.0%
!!	GATATTGACTATTGAATGTA+AGG	+	Chr2:7859956-7859975	None:intergenic	25.0%
!!!	AATTAGTTTTACTGCATCTT+TGG	-	Chr2:7860995-7861014	MsG0280006910.01.T01:intron	25.0%
!!!	AATTTTGATATAGTCAGCAA+CGG	+	Chr2:7859785-7859804	None:intergenic	25.0%
!!!	GCATGAATTTTAGAAGTATT+TGG	+	Chr2:7858405-7858424	None:intergenic	25.0%
!!!	TGTAAAATAGGCTAGTTTTA+TGG	-	Chr2:7858915-7858934	MsG0280006910.01.T01:three_prime_UTR	25.0%
	AAAAAAAATTGCAGCCAGTT+TGG	-	Chr2:7859525-7859544	MsG0280006910.01.T01:intron	30.0%
	AAATAGAATTGTTCACTTCC+TGG	-	Chr2:7859825-7859844	MsG0280006910.01.T01:intron	30.0%
	AACTTTCATCATTGGTATCT+GGG	-	Chr2:7861464-7861483	MsG0280006910.01.T01:intron	30.0%
	AATTCACGTAGTTAGCATAT+CGG	-	Chr2:7861091-7861110	MsG0280006910.01.T01:intron	30.0%
	ACAGCATCAAATCTTTAGAT+TGG	+	Chr2:7860098-7860117	None:intergenic	30.0%
	ACATCGGTGATTGTTAAATT+AGG	+	Chr2:7861200-7861219	None:intergenic	30.0%
	AGAACTATTGTTCTCATGTT+TGG	-	Chr2:7858208-7858227	MsG0280006910.01.T01:three_prime_UTR	30.0%
	ATGTATTGAAATGATTGCAG+AGG	-	Chr2:7860291-7860310	MsG0280006910.01.T01:CDS	30.0%
	ATTTAAGTGCCTATAGCATA+AGG	-	Chr2:7861815-7861834	MsG0280006910.01.T01:CDS	30.0%
	CAGAGTAAAAAAGATGATTC+TGG	-	Chr2:7861597-7861616	MsG0280006910.01.T01:intron	30.0%
	GAAGCATATATAGATATCCT+AGG	-	Chr2:7861653-7861672	MsG0280006910.01.T01:intron	30.0%
	GATGTATAAGATCATAAACC+TGG	+	Chr2:7859659-7859678	None:intergenic	30.0%
	GGGAAATCTAAGAAAAGTAA+TGG	-	Chr2:7859071-7859090	MsG0280006910.01.T01:CDS	30.0%
	GTAAAAAAGATGATTCTGGA+AGG	-	Chr2:7861601-7861620	MsG0280006910.01.T01:intron	30.0%
	TGAGATGAAAAATTCCTGAA+AGG	+	Chr2:7860967-7860986	None:intergenic	30.0%
	TGTGTGATTTATGAACAAAG+TGG	+	Chr2:7861767-7861786	None:intergenic	30.0%
!	AATATTCTGAAGGACGATAT+CGG	-	Chr2:7859576-7859595	MsG0280006910.01.T01:intron	30.0%
!	ACTTTTCTTATGCTTTTAGC+TGG	-	Chr2:7858798-7858817	MsG0280006910.01.T01:intron	30.0%
!	AGTCTTATCACTTCCATTAT+TGG	+	Chr2:7859282-7859301	None:intergenic	30.0%
!	CATCTGTAATCTAAAATGCA+AGG	+	Chr2:7859741-7859760	None:intergenic	30.0%
!	CCCTATATTTTCTAATCCTT+TGG	+	Chr2:7860388-7860407	None:intergenic	30.0%
!	TACATGTTAGTTTCTACCAT+CGG	-	Chr2:7858967-7858986	MsG0280006910.01.T01:CDS	30.0%
!	TAGTGTACTGTAAGGTTTTA+GGG	-	Chr2:7860232-7860251	MsG0280006910.01.T01:CDS	30.0%
!	TCCAATGGATTTGAGAAAAA+GGG	-	Chr2:7860757-7860776	MsG0280006910.01.T01:CDS	30.0%
!	TGTTTTACAATTTGTGACCA+GGG	-	Chr2:7859051-7859070	MsG0280006910.01.T01:CDS	30.0%
!	TTAGTGTACTGTAAGGTTTT+AGG	-	Chr2:7860231-7860250	MsG0280006910.01.T01:CDS	30.0%
!	TTATAGCTCTATTTCTCCAA+TGG	-	Chr2:7860742-7860761	MsG0280006910.01.T01:CDS	30.0%
!	TTTCAGGAATTTTTCATCTC+AGG	-	Chr2:7860966-7860985	MsG0280006910.01.T01:intron	30.0%
!	TTTTCCTTTGTGACTACATT+GGG	+	Chr2:7860919-7860938	None:intergenic	30.0%
!!	AGAATTGATTTGAATGCAGA+TGG	-	Chr2:7861378-7861397	MsG0280006910.01.T01:intron	30.0%
!!	TCCAGAGATTTTATTTCAGA+CGG	+	Chr2:7859618-7859637	None:intergenic	30.0%
!!	TTACTTTTCTTAGATTTCCC+TGG	+	Chr2:7859071-7859090	None:intergenic	30.0%
	AAATCATTCAAGTGAGCTTC+AGG	-	Chr2:7858311-7858330	MsG0280006910.01.T01:three_prime_UTR	35.0%
	AAGACCAACTCCTGAATTTA+AGG	-	Chr2:7857997-7858016	MsG0280006910.01.T01:three_prime_UTR	35.0%
	AATACAGATTTGCAATGCTG+TGG	-	Chr2:7859311-7859330	MsG0280006910.01.T01:intron	35.0%
	AGAAAAGTTCAAACCAATCC+AGG	-	Chr2:7858768-7858787	MsG0280006910.01.T01:intron	35.0%
	AGACCAACTCCTGAATTTAA+GGG	-	Chr2:7857998-7858017	MsG0280006910.01.T01:three_prime_UTR	35.0%
	AGAGTCATCCAAATTATGGA+CGG	-	Chr2:7858704-7858723	MsG0280006910.01.T01:intron	35.0%
	AGCAAGAGTCATCCAAATTA+TGG	-	Chr2:7858700-7858719	MsG0280006910.01.T01:intron	35.0%
	AGTCAAGATCATATAGGATG+AGG	+	Chr2:7859237-7859256	None:intergenic	35.0%
	AGTCATGTTCTTGACAGATT+TGG	-	Chr2:7861052-7861071	MsG0280006910.01.T01:intron	35.0%
	ATATGGTTCCGTCCATAATT+TGG	+	Chr2:7858715-7858734	None:intergenic	35.0%
	ATCCTACTAGATTTCATCGA+AGG	-	Chr2:7861278-7861297	MsG0280006910.01.T01:intron	35.0%
	ATGCATGTGCAAACAACATT+AGG	-	Chr2:7861684-7861703	MsG0280006910.01.T01:intron	35.0%
	ATGGTAAGACCTTATGCTAT+AGG	+	Chr2:7861827-7861846	None:intergenic	35.0%
	ATTGGGATAGAGTCTATTGA+AGG	+	Chr2:7860902-7860921	None:intergenic	35.0%
	CAACTTTCATCATTGGTATC+TGG	-	Chr2:7861463-7861482	MsG0280006910.01.T01:intron	35.0%
	CACTTGGATAGTTAAGTACT+TGG	-	Chr2:7860170-7860189	MsG0280006910.01.T01:intron	35.0%
	CAGATACCAATGATGAAAGT+TGG	+	Chr2:7861465-7861484	None:intergenic	35.0%
	CATAAAGATGCCTGCTTATA+TGG	+	Chr2:7858732-7858751	None:intergenic	35.0%
	CATATAAGCAGGCATCTTTA+TGG	-	Chr2:7858730-7858749	MsG0280006910.01.T01:intron	35.0%
	CTAACTACGTGAATTTGTGA+CGG	+	Chr2:7861086-7861105	None:intergenic	35.0%
	CTGAATGTAACTACATATGC+CGG	-	Chr2:7860052-7860071	MsG0280006910.01.T01:CDS	35.0%
	CTGGTCACAAATTGTAAAAC+AGG	+	Chr2:7859052-7859071	None:intergenic	35.0%
	CTTCATGATCTTTCTTGTTG+TGG	+	Chr2:7860454-7860473	None:intergenic	35.0%
	GAATAACAAGAGTTGCATTC+AGG	+	Chr2:7859350-7859369	None:intergenic	35.0%
	GTCATTCTCAGCAAAAATTG+CGG	+	Chr2:7861551-7861570	None:intergenic	35.0%
	GTTCTCATGTTTGGATTGTT+GGG	-	Chr2:7858217-7858236	MsG0280006910.01.T01:three_prime_UTR	35.0%
	TACATCTTAGTCAGTGCAAA+TGG	-	Chr2:7859095-7859114	MsG0280006910.01.T01:CDS	35.0%
	TCCTAGTCAATAAAGACACT+TGG	-	Chr2:7860154-7860173	MsG0280006910.01.T01:intron	35.0%
	TCGAAAACTTTGTTGCTACA+TGG	-	Chr2:7858121-7858140	MsG0280006910.01.T01:three_prime_UTR	35.0%
	TCTTTACAGCAATGTATGGA+AGG	-	Chr2:7859377-7859396	MsG0280006910.01.T01:intron	35.0%
	TGTTCTCATGTTTGGATTGT+TGG	-	Chr2:7858216-7858235	MsG0280006910.01.T01:three_prime_UTR	35.0%
	TTATGGAGGAAACTCAAGAA+TGG	-	Chr2:7860873-7860892	MsG0280006910.01.T01:intron	35.0%
	TTCAAAACCATTCCATCCTA+TGG	+	Chr2:7861575-7861594	None:intergenic	35.0%
!	CCAATGGATTTGAGAAAAAG+GGG	-	Chr2:7860758-7860777	MsG0280006910.01.T01:CDS	35.0%
!	CTCCAATGGATTTGAGAAAA+AGG	-	Chr2:7860756-7860775	MsG0280006910.01.T01:CDS	35.0%
!	CTGTTTTACAATTTGTGACC+AGG	-	Chr2:7859050-7859069	MsG0280006910.01.T01:CDS	35.0%
!	GGAATTTTTCATCTCAGGTA+TGG	-	Chr2:7860971-7860990	MsG0280006910.01.T01:intron	35.0%
!	GTTTTCCTTTGTGACTACAT+TGG	+	Chr2:7860920-7860939	None:intergenic	35.0%
!	TCCAAGTGTCTTTATTGACT+AGG	+	Chr2:7860158-7860177	None:intergenic	35.0%
!	TGAACTTTTCTTTGTGCACA+AGG	+	Chr2:7858759-7858778	None:intergenic	35.0%
!!	CCAGAGATTTTATTTCAGAC+GGG	+	Chr2:7859617-7859636	None:intergenic	35.0%
!!	TGGTCAAGTTTGTTAATGCA+AGG	+	Chr2:7861747-7861766	None:intergenic	35.0%
!!!	AGCCAGCTTGTTGAATTTTT+GGG	+	Chr2:7860355-7860374	None:intergenic	35.0%
	AATGACACCATAGGATGGAA+TGG	-	Chr2:7861565-7861584	MsG0280006910.01.T01:intron	40.0%
	AATGCTGGCATAAGGAGTTA+TGG	-	Chr2:7861628-7861647	MsG0280006910.01.T01:intron	40.0%
	ATCTTAGTCAGTGCAAATGG+TGG	-	Chr2:7859098-7859117	MsG0280006910.01.T01:CDS	40.0%
	ATGTTAGACACTCAGTTGCT+AGG	-	Chr2:7860419-7860438	MsG0280006910.01.T01:intron	40.0%
	CAACGAGTTGCTGTAAGTAT+TGG	-	Chr2:7859131-7859150	MsG0280006910.01.T01:intron	40.0%
	CCTGATATATGTCACCAAAC+TGG	+	Chr2:7859542-7859561	None:intergenic	40.0%
	CTATCCCAATGTAGTCACAA+AGG	-	Chr2:7860912-7860931	MsG0280006910.01.T01:intron	40.0%
	CTTGTGACCTGAATATCAGA+AGG	+	Chr2:7859882-7859901	None:intergenic	40.0%
	GAAAGATCATGAAGCTGCAA+AGG	-	Chr2:7860460-7860479	MsG0280006910.01.T01:intron	40.0%
	GCATGCATCAATCTGAAAGT+TGG	+	Chr2:7858586-7858605	None:intergenic	40.0%
	GTAAAATAGTCGTGTACCGA+TGG	+	Chr2:7858986-7859005	None:intergenic	40.0%
	GTCCCAAAAATTCAACAAGC+TGG	-	Chr2:7860350-7860369	MsG0280006910.01.T01:intron	40.0%
	GTCCTTCGATGAAATCTAGT+AGG	+	Chr2:7861283-7861302	None:intergenic	40.0%
	TACAGATGCAGTTCTTGCAA+AGG	-	Chr2:7859753-7859772	MsG0280006910.01.T01:intron	40.0%
	TAGGGATCTCACTTGCAATT+AGG	-	Chr2:7860250-7860269	MsG0280006910.01.T01:CDS	40.0%
	TATTCCATGTGTGAGTTTGG+AGG	-	Chr2:7860535-7860554	MsG0280006910.01.T01:CDS	40.0%
	TCCAAACTCACACATGGAAT+AGG	+	Chr2:7860536-7860555	None:intergenic	40.0%
	TCCTATGCCTTCTGATATTC+AGG	-	Chr2:7859872-7859891	MsG0280006910.01.T01:intron	40.0%
	TGGTAGCCAACTTTCATCAT+TGG	-	Chr2:7861456-7861475	MsG0280006910.01.T01:intron	40.0%
	TGTTAGACACTCAGTTGCTA+GGG	-	Chr2:7860420-7860439	MsG0280006910.01.T01:intron	40.0%
	TTGGGACAAACTTCAAAGCA+TGG	+	Chr2:7860337-7860356	None:intergenic	40.0%
	TTTGATATAGTCAGCAACGG+TGG	+	Chr2:7859782-7859801	None:intergenic	40.0%
	TTTGCTGAGAATGACACCAT+AGG	-	Chr2:7861556-7861575	MsG0280006910.01.T01:intron	40.0%
!	ACCTGAATATCAGAAGGCAT+AGG	+	Chr2:7859876-7859895	None:intergenic	40.0%
!	CCAGTTTGGTGACATATATC+AGG	-	Chr2:7859539-7859558	MsG0280006910.01.T01:intron	40.0%
!	CCCCTTTTTCTCAAATCCAT+TGG	+	Chr2:7860761-7860780	None:intergenic	40.0%
!	CCCGTCTGAAATAAAATCTC+TGG	-	Chr2:7859614-7859633	MsG0280006910.01.T01:intron	40.0%
!	CGATTGTTTCTCAGTGATGT+AGG	+	Chr2:7858502-7858521	None:intergenic	40.0%
!	CTTGGCTAGTTCTCGTATTA+TGG	-	Chr2:7860856-7860875	MsG0280006910.01.T01:intron	40.0%
!	GAATGATTTGAAGCTGCACT+TGG	+	Chr2:7858300-7858319	None:intergenic	40.0%
!	TGTTGTTTGCACATGCATTC+TGG	+	Chr2:7861682-7861701	None:intergenic	40.0%
!!	AATTCAGGAGTTGGTCTTCT+TGG	+	Chr2:7857995-7858014	None:intergenic	40.0%
!!	GTATTTTCCATGACTGACTC+TGG	-	Chr2:7861436-7861455	MsG0280006910.01.T01:intron	40.0%
!!!	GAGCCAGCTTGTTGAATTTT+TGG	+	Chr2:7860356-7860375	None:intergenic	40.0%
	AACTTCAAGCCTACCGAGAA+AGG	-	Chr2:7860578-7860597	MsG0280006910.01.T01:intron	45.0%
	AAGGTCAGAATGCTGGCATA+AGG	-	Chr2:7861620-7861639	MsG0280006910.01.T01:intron	45.0%
	AGAGAGGGAAATGCCTTTCT+CGG	+	Chr2:7860594-7860613	None:intergenic	45.0%
	AGATGCAGTTCTTGCAAAGG+AGG	-	Chr2:7859756-7859775	MsG0280006910.01.T01:intron	45.0%
	AGCCGATCGTACATATGTAC+AGG	+	Chr2:7858525-7858544	None:intergenic	45.0%
	AGGAGGTGAGAATGAGAGAA+AGG	-	Chr2:7860555-7860574	MsG0280006910.01.T01:intron	45.0%
	ATGGACGGAACCATATAAGC+AGG	-	Chr2:7858719-7858738	MsG0280006910.01.T01:intron	45.0%
	CAAGTTCAGTGGTGGTAAGA+AGG	+	Chr2:7860942-7860961	None:intergenic	45.0%
	CATTCACGCAGTCAGTACAT+CGG	+	Chr2:7861216-7861235	None:intergenic	45.0%
	CCTGGATATGCAAATCGACT+TGG	-	Chr2:7859843-7859862	MsG0280006910.01.T01:intron	45.0%
	CGCCTGTACATATGTACGAT+CGG	-	Chr2:7858520-7858539	MsG0280006910.01.T01:intron	45.0%
	CTAGCAGCACCCTTAAATTC+AGG	+	Chr2:7858010-7858029	None:intergenic	45.0%
	CTGAGAATGACACCATAGGA+TGG	-	Chr2:7861560-7861579	MsG0280006910.01.T01:intron	45.0%
	GCAATGTATGGAAGGATCCA+AGG	-	Chr2:7859385-7859404	MsG0280006910.01.T01:intron	45.0%
	GCACCCTTAAATTCAGGAGT+TGG	+	Chr2:7858004-7858023	None:intergenic	45.0%
	GCCTATTCCATGTGTGAGTT+TGG	-	Chr2:7860532-7860551	MsG0280006910.01.T01:CDS	45.0%
	GGACGATATCGGTATTGTGA+AGG	-	Chr2:7859587-7859606	MsG0280006910.01.T01:intron	45.0%
	TCAAGCGCTCAAGAAAGAGA+GGG	+	Chr2:7860609-7860628	None:intergenic	45.0%
	TCTCTGGATGCAGAAGCAAT+TGG	-	Chr2:7859630-7859649	MsG0280006910.01.T01:intron	45.0%
	TCTGGGTTCAGAACCTACTA+TGG	-	Chr2:7861481-7861500	MsG0280006910.01.T01:intron	45.0%
	TGGAGGAAACTCAAGAATGG+AGG	-	Chr2:7860876-7860895	MsG0280006910.01.T01:intron	45.0%
	TTCAAGCGCTCAAGAAAGAG+AGG	+	Chr2:7860610-7860629	None:intergenic	45.0%
	TTGAATGCAGATGGCTGCTT+TGG	-	Chr2:7861387-7861406	MsG0280006910.01.T01:intron	45.0%
!	ATTCTGGAAGGTCAGAATGC+TGG	-	Chr2:7861613-7861632	MsG0280006910.01.T01:intron	45.0%
!	CTGGCTCTCTATTGATCCAA+AGG	-	Chr2:7860369-7860388	MsG0280006910.01.T01:intron	45.0%
!	GGCTAGTTCTCGTATTATGG+AGG	-	Chr2:7860859-7860878	MsG0280006910.01.T01:intron	45.0%
!	GTACGATCGGCTTCTAAACT+TGG	-	Chr2:7858533-7858552	MsG0280006910.01.T01:intron	45.0%
!!	AGAACAAGTGCTGCTTCTTC+TGG	+	Chr2:7860800-7860819	None:intergenic	45.0%
!!	CCAAGTCGATTTGCATATCC+AGG	+	Chr2:7859846-7859865	None:intergenic	45.0%
!!	GAATTTAAGGGTGCTGCTAG+AGG	-	Chr2:7858010-7858029	MsG0280006910.01.T01:three_prime_UTR	45.0%
!!	TATTATTATTAAAAGTAATA+TGG	+	Chr2:7861339-7861358	None:intergenic	5.0%
!!!	TATTATTTTACATTTTTTAA+AGG	+	Chr2:7858889-7858908	None:intergenic	5.0%
	ACCTCCTCCAAACTCACACA+TGG	+	Chr2:7860542-7860561	None:intergenic	50.0%
	AGGGAAATGCCTTTCTCGGT+AGG	+	Chr2:7860590-7860609	None:intergenic	50.0%
	AGTTGCCAAGTGTCAGACAC+CGG	+	Chr2:7860074-7860093	None:intergenic	50.0%
	ATATGCCGGTGTCTGACACT+TGG	-	Chr2:7860066-7860085	MsG0280006910.01.T01:CDS	50.0%
	CACATGCATTCTGGACACCT+AGG	+	Chr2:7861673-7861692	None:intergenic	50.0%
	CCACTGAACTTGCACCTTTC+AGG	-	Chr2:7860950-7860969	MsG0280006910.01.T01:intron	50.0%
	GAAGCAGCACTTGTTCTAGC+AGG	-	Chr2:7860803-7860822	MsG0280006910.01.T01:intron	50.0%
	GATTTGCAATGCTGTGGCTG+TGG	-	Chr2:7859317-7859336	MsG0280006910.01.T01:intron	50.0%
	GCACTTGTTCTAGCAGGTCT+TGG	-	Chr2:7860809-7860828	MsG0280006910.01.T01:intron	50.0%
	GGGTTCAGAACCTACTATGG+CGG	-	Chr2:7861484-7861503	MsG0280006910.01.T01:intron	50.0%
	TCCATGTGTGAGTTTGGAGG+AGG	-	Chr2:7860538-7860557	MsG0280006910.01.T01:CDS	50.0%
	TGACAGCGGTTGATTGTCGT+TGG	-	Chr2:7858095-7858114	MsG0280006910.01.T01:three_prime_UTR	50.0%
	TTCAGAACCTACTATGGCGG+CGG	-	Chr2:7861487-7861506	MsG0280006910.01.T01:intron	50.0%
	TTGGCTACCAGAGTCAGTCA+TGG	+	Chr2:7861446-7861465	None:intergenic	50.0%
!	CCTGAAAGGTGCAAGTTCAG+TGG	+	Chr2:7860953-7860972	None:intergenic	50.0%
!	GAAAGGTGCAAGTTCAGTGG+TGG	+	Chr2:7860950-7860969	None:intergenic	50.0%
!	GCTGCTTCTTCTGGTGTCAA+TGG	+	Chr2:7860791-7860810	None:intergenic	50.0%
!	TCATACCTCTGCAAGAGCAC+TGG	+	Chr2:7858562-7858581	None:intergenic	50.0%
!	TGCAGAAGCAATTGGTAGCC+AGG	-	Chr2:7859638-7859657	MsG0280006910.01.T01:intron	50.0%
!!	GGTGCTGCTAGAGGAATGTT+AGG	-	Chr2:7858019-7858038	MsG0280006910.01.T01:three_prime_UTR	50.0%
!!	GTACTGACTGCGTGAATGTG+TGG	-	Chr2:7861218-7861237	MsG0280006910.01.T01:intron	50.0%
	ATGATCCACCGTCGTGGAAG+AGG	-	Chr2:7858071-7858090	MsG0280006910.01.T01:three_prime_UTR	55.0%
	CAAGCAATGATCCACCGTCG+TGG	-	Chr2:7858065-7858084	MsG0280006910.01.T01:three_prime_UTR	55.0%
	GCTGTCATCCTCTTCCACGA+CGG	+	Chr2:7858082-7858101	None:intergenic	55.0%
	GTCGTGGAAGAGGATGACAG+CGG	-	Chr2:7858081-7858100	MsG0280006910.01.T01:three_prime_UTR	55.0%
!!	GCGGCTAGCCTTGTCTTGTT+CGG	+	Chr2:7861532-7861551	None:intergenic	55.0%
	AGCATGACCGCCGCCATAGT+AGG	+	Chr2:7861497-7861516	None:intergenic	60.0%
	CCTTGCGGCCGAACAAGACA+AGG	-	Chr2:7861521-7861540	MsG0280006910.01.T01:intron	60.0%
	GCGGTCATGCTCCTACCTTG+CGG	-	Chr2:7861506-7861525	MsG0280006910.01.T01:intron	60.0%
	GTCATCCTCTTCCACGACGG+TGG	+	Chr2:7858079-7858098	None:intergenic	60.0%
!	CCTTGTCTTGTTCGGCCGCA+AGG	+	Chr2:7861524-7861543	None:intergenic	60.0%
!	GTCTTGTTCGGCCGCAAGGT+AGG	+	Chr2:7861520-7861539	None:intergenic	60.0%
	GGCGTCCAGTGCTCTTGCAG+AGG	-	Chr2:7858554-7858573	MsG0280006910.01.T01:intron	65.0%

Chromosome	Type	Strat	End	Strand	Name
Chr2	gene	7857974	7861908	7857974	ID=MsG0280006910.01;Name=MsG0280006910.01
Chr2	mRNA	7857974	7861908	7857974	ID=MsG0280006910.01.T01;Parent=MsG0280006910.01;Name=MsG0280006910.01.T01;_AED=0.32;_eAED=0.32;_QI=0\|0.75\|0.88\|1\|0.75\|0.77\|9\|574\|305
Chr2	exon	7857974	7858485	7857974	ID=MsG0280006910.01.T01:exon:8008;Parent=MsG0280006910.01.T01
Chr2	exon	7858895	7859134	7858895	ID=MsG0280006910.01.T01:exon:8007;Parent=MsG0280006910.01.T01
Chr2	exon	7859989	7860131	7859989	ID=MsG0280006910.01.T01:exon:8006;Parent=MsG0280006910.01.T01
Chr2	exon	7860223	7860343	7860223	ID=MsG0280006910.01.T01:exon:8005;Parent=MsG0280006910.01.T01
Chr2	exon	7860476	7860564	7860476	ID=MsG0280006910.01.T01:exon:8004;Parent=MsG0280006910.01.T01
Chr2	exon	7860740	7860810	7860740	ID=MsG0280006910.01.T01:exon:8003;Parent=MsG0280006910.01.T01
Chr2	exon	7861093	7861192	7861093	ID=MsG0280006910.01.T01:exon:8002;Parent=MsG0280006910.01.T01
Chr2	exon	7861307	7861393	7861307	ID=MsG0280006910.01.T01:exon:8001;Parent=MsG0280006910.01.T01
Chr2	exon	7861780	7861908	7861780	ID=MsG0280006910.01.T01:exon:8000;Parent=MsG0280006910.01.T01
Chr2	CDS	7861780	7861908	7861780	ID=MsG0280006910.01.T01:cds;Parent=MsG0280006910.01.T01
Chr2	CDS	7861307	7861393	7861307	ID=MsG0280006910.01.T01:cds;Parent=MsG0280006910.01.T01
Chr2	CDS	7861093	7861192	7861093	ID=MsG0280006910.01.T01:cds;Parent=MsG0280006910.01.T01
Chr2	CDS	7860740	7860810	7860740	ID=MsG0280006910.01.T01:cds;Parent=MsG0280006910.01.T01
Chr2	CDS	7860476	7860564	7860476	ID=MsG0280006910.01.T01:cds;Parent=MsG0280006910.01.T01
Chr2	CDS	7860223	7860343	7860223	ID=MsG0280006910.01.T01:cds;Parent=MsG0280006910.01.T01
Chr2	CDS	7859989	7860131	7859989	ID=MsG0280006910.01.T01:cds;Parent=MsG0280006910.01.T01
Chr2	CDS	7858957	7859134	7858957	ID=MsG0280006910.01.T01:cds;Parent=MsG0280006910.01.T01
Chr2	three_prime_UTR	7858895	7858956	7858895	ID=MsG0280006910.01.T01:three_prime_utr;Parent=MsG0280006910.01.T01
Chr2	three_prime_UTR	7857974	7858485	7857974	ID=MsG0280006910.01.T01:three_prime_utr;Parent=MsG0280006910.01.T01

Gene Sequence

>MsG0280006910.01.T01

ATGGGAAAACAAGGGTCTCCAAGAAGACCAACTCCTGAATTTAAGGGTGCTGCTAGAGGAATGTTAGGAATGAATAAAGATTACAAGATAACAAGCAATGATCCACCGTCGTGGAAGAGGATGACAGCGGATACAGATTCCTACATCACTGAGAAACAATCGCCTGTACATATGTACGATCGGCTTCTAAACTTGGCGTCCAGTGCTCTTGCAGAGAAAGAGTTCAAGCAAGAGTCATCCAAATTATGGACGGAACCATATAAGCAGGCATCTTTATGGAAACCTTGTGCACAAAGAAAAGTTCAAACCAATCCAGGGAAATCTAAGAAAAGTAATGGCTACATCTTAGTCAGTGCAAATGGTGGTCTTAATCAACAACGAGTTGCTATTTGCAATGCTGTGGCTGTGGCATCTGTCCTGAATGCAACTCTTGTTATTCCAAAATTTCTTTACAGCAATGTATGGAAGGATCCAAGCCAGTTTGGTGACATATATCAGGAAGAGTATTTCATAAATATTCTGAAGGACGATATCGGTATTGTGAAGGAACTCCCGTCTGAAATAAAATCTCTGGATGCAGAAGCAATTGGTAGCCAGATTACAGATGCAGTTCTTGCAAAGGAGGCCACCGTTGCTGACTATATCAAAATTGTGCTTCCTCTTCTTTTGAAAAATAGAATTGTTCACTTCCTGGATATGCAAATCGACTTGGTTTTGATCCTATGCCTTCTGATATTCAGCTCTATTTCTCCAATGGATTTGAGAAAAAGGGGAAGATGTCCATTGACACCAGAAGAAGCAGCACTTGTTCTAGCAGGTCTTGGTTTCAAACAAGAAACTTACATTTACTTGGCTAGTTCTCGTATTATGGAGGAAACTCAAGAATGGAGGCCTTCAATAGACTCTATCCCAATGTAG

Protein sequence

>MsG0280006910.01.T01

MGKQGSPRRPTPEFKGAARGMLGMNKDYKITSNDPPSWKRMTADTDSYITEKQSPVHMYDRLLNLASSALAEKEFKQESSKLWTEPYKQASLWKPCAQRKVQTNPGKSKKSNGYILVSANGGLNQQRVAICNAVAVASVLNATLVIPKFLYSNVWKDPSQFGDIYQEEYFINILKDDIGIVKELPSEIKSLDAEAIGSQITDAVLAKEATVADYIKIVLPLLLKNRIVHFLDMQIDLVLILCLLIFSSISPMDLRKRGRCPLTPEEAALVLAGLGFKQETYIYLASSRIMEETQEWRPSIDSIPM*