Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880046286.01.T01 | PNY09850.1 | 72.121 | 165 | 20 | 1 | 1 | 139 | 35 | 199 | 1.63E-73 | 238 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880046286.01.T01 | Q9STK5 | 42.331 | 163 | 66 | 2 | 5 | 139 | 177 | 339 | 4.07E-37 | 136 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880046286.01.T01 | A0A2K3P3J6 | 72.121 | 165 | 20 | 1 | 1 | 139 | 35 | 199 | 7.80e-74 | 238 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0880043479.01 | MsG0880046286.01 | 0.801149 | 1.031794e-48 | 4.891816e-46 |
| MsG0580025135.01 | MsG0880046286.01 | 0.809493 | 1.841660e-50 | 1.082852e-47 |
| MsG0480018163.01 | MsG0880046286.01 | 0.800999 | 1.107346e-48 | 5.230449e-46 |
| MsG0580029008.01 | MsG0880046286.01 | 0.807699 | 4.449708e-50 | 2.495223e-47 |
| MsG0780040057.01 | MsG0880046286.01 | 0.803167 | 3.966269e-49 | 1.979361e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880046286.01.T01 | MTR_4g104830 | 70.732 | 164 | 23 | 1 | 1 | 139 | 178 | 341 | 6.61e-75 | 234 |
| MsG0880046286.01.T01 | MTR_4g104830 | 34.884 | 86 | 56 | 0 | 35 | 120 | 100 | 185 | 6.34e-12 | 62.0 |
| MsG0880046286.01.T01 | MTR_4g104800 | 68.902 | 164 | 26 | 1 | 1 | 139 | 181 | 344 | 1.38e-71 | 226 |
| MsG0880046286.01.T01 | MTR_4g104800 | 34.314 | 102 | 64 | 1 | 35 | 133 | 103 | 204 | 6.57e-14 | 67.8 |
| MsG0880046286.01.T01 | MTR_4g104860 | 87.500 | 104 | 13 | 0 | 36 | 139 | 1 | 104 | 1.06e-65 | 199 |
| MsG0880046286.01.T01 | MTR_5g092040 | 58.683 | 167 | 37 | 3 | 1 | 139 | 111 | 273 | 2.27e-55 | 181 |
| MsG0880046286.01.T01 | MTR_4g104790 | 54.268 | 164 | 50 | 1 | 1 | 139 | 176 | 339 | 7.70e-52 | 173 |
| MsG0880046286.01.T01 | MTR_0252s0060 | 41.727 | 139 | 34 | 4 | 1 | 139 | 113 | 204 | 4.29e-20 | 85.5 |
| MsG0880046286.01.T01 | MTR_7g018240 | 40.404 | 99 | 59 | 0 | 35 | 133 | 228 | 326 | 6.86e-20 | 84.7 |
| MsG0880046286.01.T01 | MTR_1g108270 | 29.524 | 105 | 73 | 1 | 31 | 135 | 90 | 193 | 8.99e-12 | 61.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880046286.01.T01 | AT3G48250 | 42.331 | 163 | 66 | 2 | 5 | 139 | 177 | 339 | 4.15e-38 | 136 |
| MsG0880046286.01.T01 | AT3G48250 | 38.298 | 94 | 55 | 1 | 35 | 125 | 95 | 188 | 3.74e-12 | 62.8 |
| MsG0880046286.01.T01 | AT3G02490 | 32.432 | 111 | 74 | 1 | 30 | 139 | 248 | 358 | 1.56e-17 | 78.2 |
| MsG0880046286.01.T01 | AT5G15980 | 33.036 | 112 | 73 | 1 | 30 | 139 | 254 | 365 | 2.26e-16 | 75.1 |
Find 26 sgRNAs with CRISPR-Local
Find 35 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAGGTGGTTAATATTATTTC+AGG | 0.189574 | 8:+71549037 | MsG0880046286.01.T01:CDS |
| TGGAGTCAATTGAGGAGTTT+TGG | 0.260134 | 8:+71549259 | MsG0880046286.01.T01:CDS |
| GCTCTTAAGAATTTCTATAA+AGG | 0.284967 | 8:+71548977 | MsG0880046286.01.T01:CDS |
| TCCTCAATTGACTCCATTCT+AGG | 0.310863 | 8:-71549252 | None:intergenic |
| CATTGGGTTGGGAAGCAATC+TGG | 0.343290 | 8:+71549183 | MsG0880046286.01.T01:CDS |
| TTCATATAAGCCATCAGTTC+CGG | 0.350670 | 8:+71549413 | MsG0880046286.01.T01:CDS |
| CTCTTAAGAATTTCTATAAA+GGG | 0.363103 | 8:+71548978 | MsG0880046286.01.T01:CDS |
| GAAGAGTGTCGGCCATGAAT+TGG | 0.369757 | 8:+71549299 | MsG0880046286.01.T01:CDS |
| GGCTTATAAGTTCTTTCATT+GGG | 0.370007 | 8:+71549167 | MsG0880046286.01.T01:CDS |
| GCTTCAAAATAGTCCTTTGA+AGG | 0.381775 | 8:+71549146 | MsG0880046286.01.T01:CDS |
| AGGCTTATAAGTTCTTTCAT+TGG | 0.383452 | 8:+71549166 | MsG0880046286.01.T01:CDS |
| AAGTATCGAAATCCAATTCA+TGG | 0.400179 | 8:-71549311 | None:intergenic |
| TATAAGTTCTTTCATTGGGT+TGG | 0.401404 | 8:+71549171 | MsG0880046286.01.T01:CDS |
| ATAAGTTCTTTCATTGGGTT+GGG | 0.403998 | 8:+71549172 | MsG0880046286.01.T01:CDS |
| ATTGCAAAGTGTTGTCAAAA+AGG | 0.405296 | 8:+71549018 | MsG0880046286.01.T01:CDS |
| TGCTAGAGTTCTTCCTAGAA+TGG | 0.434558 | 8:+71549239 | MsG0880046286.01.T01:CDS |
| TCCTAGAATGGAGTCAATTG+AGG | 0.489128 | 8:+71549251 | MsG0880046286.01.T01:CDS |
| ATCTGGTTATCAACGGAATA+CGG | 0.493053 | 8:+71549200 | MsG0880046286.01.T01:CDS |
| CAAGCTTTACGAACATATGA+TGG | 0.496321 | 8:+71549386 | MsG0880046286.01.T01:CDS |
| AGGTGGTTAATATTATTTCA+GGG | 0.501532 | 8:+71549038 | MsG0880046286.01.T01:CDS |
| CTTTAAAGCATAATGCAATC+CGG | 0.525645 | 8:-71549432 | None:intergenic |
| ATAATGCAATCCGGAACTGA+TGG | 0.565045 | 8:-71549423 | None:intergenic |
| AAGAACTTATAAGCCTTCAA+AGG | 0.584866 | 8:-71549159 | None:intergenic |
| GGAAGCAATCTGGTTATCAA+CGG | 0.630177 | 8:+71549193 | MsG0880046286.01.T01:CDS |
| ATACTTACATAGAGATATCG+AGG | 0.649236 | 8:+71549328 | MsG0880046286.01.T01:CDS |
| CTTGAGGAGATGAAGAGTGT+CGG | 0.656126 | 8:+71549288 | MsG0880046286.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | CTCTTAAGAATTTCTATAAA+GGG | + | Chr8:71548978-71548997 | MsG0880046286.01.T01:CDS | 20.0% |
| ! | AAGGTGGTTAATATTATTTC+AGG | + | Chr8:71549037-71549056 | MsG0880046286.01.T01:CDS | 25.0% |
| ! | AGGTGGTTAATATTATTTCA+GGG | + | Chr8:71549038-71549057 | MsG0880046286.01.T01:CDS | 25.0% |
| ! | GAAGGTTAAAATTCAACTTT+CGG | + | Chr8:71549096-71549115 | MsG0880046286.01.T01:intron | 25.0% |
| ! | GCTCTTAAGAATTTCTATAA+AGG | + | Chr8:71548977-71548996 | MsG0880046286.01.T01:CDS | 25.0% |
| !! | TTTCGGATAATTTGTGATTA+GGG | + | Chr8:71549113-71549132 | MsG0880046286.01.T01:intron | 25.0% |
| AAGAACTTATAAGCCTTCAA+AGG | - | Chr8:71549162-71549181 | None:intergenic | 30.0% | |
| AGGCTTATAAGTTCTTTCAT+TGG | + | Chr8:71549166-71549185 | MsG0880046286.01.T01:CDS | 30.0% | |
| ATAAGTTCTTTCATTGGGTT+GGG | + | Chr8:71549172-71549191 | MsG0880046286.01.T01:CDS | 30.0% | |
| ATACTTACATAGAGATATCG+AGG | + | Chr8:71549328-71549347 | MsG0880046286.01.T01:CDS | 30.0% | |
| ATATTATTTCAGGGTCTGAT+TGG | + | Chr8:71549047-71549066 | MsG0880046286.01.T01:intron | 30.0% | |
| GGCTTATAAGTTCTTTCATT+GGG | + | Chr8:71549167-71549186 | MsG0880046286.01.T01:CDS | 30.0% | |
| TATAAGTTCTTTCATTGGGT+TGG | + | Chr8:71549171-71549190 | MsG0880046286.01.T01:CDS | 30.0% | |
| TATTATTTCAGGGTCTGATT+GGG | + | Chr8:71549048-71549067 | MsG0880046286.01.T01:intron | 30.0% | |
| ! | CTTTCGGATAATTTGTGATT+AGG | + | Chr8:71549112-71549131 | MsG0880046286.01.T01:intron | 30.0% |
| !! | AAGTATCGAAATCCAATTCA+TGG | - | Chr8:71549314-71549333 | None:intergenic | 30.0% |
| !! | ATTGCAAAGTGTTGTCAAAA+AGG | + | Chr8:71549018-71549037 | MsG0880046286.01.T01:CDS | 30.0% |
| ATCTGGTTATCAACGGAATA+CGG | + | Chr8:71549200-71549219 | MsG0880046286.01.T01:CDS | 35.0% | |
| CAAGCTTTACGAACATATGA+TGG | + | Chr8:71549386-71549405 | MsG0880046286.01.T01:CDS | 35.0% | |
| GCTTCAAAATAGTCCTTTGA+AGG | + | Chr8:71549146-71549165 | MsG0880046286.01.T01:CDS | 35.0% | |
| TTCATATAAGCCATCAGTTC+CGG | + | Chr8:71549413-71549432 | MsG0880046286.01.T01:CDS | 35.0% | |
| ATAATGCAATCCGGAACTGA+TGG | - | Chr8:71549426-71549445 | None:intergenic | 40.0% | |
| GGAAGCAATCTGGTTATCAA+CGG | + | Chr8:71549193-71549212 | MsG0880046286.01.T01:CDS | 40.0% | |
| TCCTAGAATGGAGTCAATTG+AGG | + | Chr8:71549251-71549270 | MsG0880046286.01.T01:CDS | 40.0% | |
| TGATAAGGTTGAGAATGCAC+TGG | + | Chr8:71549072-71549091 | MsG0880046286.01.T01:intron | 40.0% | |
| TGCTAGAGTTCTTCCTAGAA+TGG | + | Chr8:71549239-71549258 | MsG0880046286.01.T01:CDS | 40.0% | |
| ! | TCCTCAATTGACTCCATTCT+AGG | - | Chr8:71549255-71549274 | None:intergenic | 40.0% |
| ! | TGGAGTCAATTGAGGAGTTT+TGG | + | Chr8:71549259-71549278 | MsG0880046286.01.T01:CDS | 40.0% |
| !! | GCAAAGTGTTGTCAAAAAGG+TGG | + | Chr8:71549021-71549040 | MsG0880046286.01.T01:CDS | 40.0% |
| !!! | GGAGTTTTGGAGTATTCTTG+AGG | + | Chr8:71549272-71549291 | MsG0880046286.01.T01:CDS | 40.0% |
| AGGGTCTGATTGGGATGATA+AGG | + | Chr8:71549057-71549076 | MsG0880046286.01.T01:intron | 45.0% | |
| ! | CTTGAGGAGATGAAGAGTGT+CGG | + | Chr8:71549288-71549307 | MsG0880046286.01.T01:CDS | 45.0% |
| GGTTGAGAATGCACTGGAGA+AGG | + | Chr8:71549078-71549097 | MsG0880046286.01.T01:intron | 50.0% | |
| !! | CATTGGGTTGGGAAGCAATC+TGG | + | Chr8:71549183-71549202 | MsG0880046286.01.T01:CDS | 50.0% |
| !! | GAAGAGTGTCGGCCATGAAT+TGG | + | Chr8:71549299-71549318 | MsG0880046286.01.T01:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr8 | gene | 71548959 | 71549452 | 71548959 | ID=MsG0880046286.01;Name=MsG0880046286.01 |
| Chr8 | mRNA | 71548959 | 71549452 | 71548959 | ID=MsG0880046286.01.T01;Parent=MsG0880046286.01;Name=MsG0880046286.01.T01;_AED=0.49;_eAED=0.49;_QI=0|0|0|1|1|1|2|0|139 |
| Chr8 | exon | 71548959 | 71549059 | 71548959 | ID=MsG0880046286.01.T01:exon:27844;Parent=MsG0880046286.01.T01 |
| Chr8 | exon | 71549134 | 71549452 | 71549134 | ID=MsG0880046286.01.T01:exon:27845;Parent=MsG0880046286.01.T01 |
| Chr8 | CDS | 71548959 | 71549059 | 71548959 | ID=MsG0880046286.01.T01:cds;Parent=MsG0880046286.01.T01 |
| Chr8 | CDS | 71549134 | 71549452 | 71549134 | ID=MsG0880046286.01.T01:cds;Parent=MsG0880046286.01.T01 |
| Gene Sequence |
| Protein sequence |
