AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0880043479.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880043479.01.T01 MTR_8g036220 93.182 44 3 0 1 44 125 168 1.90e-22 85.5
MsG0880043479.01.T01 MTR_7g051940 68.182 44 14 0 1 44 122 165 9.16e-15 65.1
MsG0880043479.01.T01 MTR_7g405790 65.909 44 15 0 1 44 124 167 6.72e-14 62.8
MsG0880043479.01.T01 MTR_8g089985 54.902 51 23 0 1 51 126 176 1.95e-12 58.9
MsG0880043479.01.T01 MTR_1g078200 69.048 42 13 0 1 42 123 164 3.37e-12 58.5
MsG0880043479.01.T01 MTR_0262s0050 50.980 51 25 0 1 51 124 174 4.24e-12 58.2
MsG0880043479.01.T01 MTR_3g086980 52.941 51 24 0 1 51 125 175 6.48e-12 57.8
MsG0880043479.01.T01 MTR_1g052475 49.020 51 26 0 1 51 124 174 7.26e-12 57.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880043479.01.T01 AT5G46025 57.143 56 24 0 1 56 54 109 2.85e-14 61.6
MsG0880043479.01.T01 AT5G47960 56.863 51 22 0 1 51 124 174 3.26e-14 63.9
MsG0880043479.01.T01 AT1G05810 57.143 56 23 1 1 55 121 176 1.00e-13 62.4
MsG0880043479.01.T01 AT2G31680 65.909 44 15 0 1 44 121 164 1.32e-13 62.0
MsG0880043479.01.T01 AT1G05810 63.636 44 16 0 1 44 167 210 1.78e-13 62.4
MsG0880043479.01.T01 AT2G43130 63.636 44 16 0 1 44 121 164 1.98e-13 61.6
MsG0880043479.01.T01 AT3G07410 61.364 44 17 0 1 44 121 164 6.83e-13 60.1
MsG0880043479.01.T01 AT5G47520 66.667 42 14 0 1 42 123 164 2.49e-12 58.9
MsG0880043479.01.T01 AT4G39990 58.140 43 18 0 1 43 126 168 3.12e-12 58.5
MsG0880043479.01.T01 AT4G18430 55.814 43 19 0 1 43 122 164 4.45e-12 58.2
MsG0880043479.01.T01 AT3G12160 50.980 51 25 0 1 51 124 174 6.45e-12 57.8
MsG0880043479.01.T01 AT1G18200 65.116 43 15 0 1 43 122 164 1.78e-11 56.6
MsG0880043479.01.T01 AT5G65270 49.020 51 26 0 1 51 126 176 1.94e-11 56.2

Find 8 sgRNAs with CRISPR-Local

Find 8 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTCCTCGATAGGCACTTCT+CGG 0.362301 8:+25389629 None:intergenic
AACATAGCTGTCATGCTTGT+TGG 0.371700 8:-25389676 None:intergenic
ACATATTGTGAGAGAAGAAC+AGG 0.437269 8:+25389528 None:intergenic
AAGTCTTTGGCTTCCTCGAT+AGG 0.469086 8:+25389619 None:intergenic
GTGAGAGAAGAACAGGAAAG+TGG 0.591349 8:+25389535 None:intergenic
GCACTTCTCGGATTGAACTC+AGG 0.592554 8:+25389641 None:intergenic
AAAGACTTAGCAAAACAGAA+AGG 0.614557 8:-25389604 MsG0880043479.01.T01:CDS
AATCCGAGAAGTGCCTATCG+AGG 0.678608 8:-25389632 MsG0880043479.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
AAAGACTTAGCAAAACAGAA+AGG - Chr8:25389576-25389595 MsG0880043479.01.T01:CDS 30.0%
! TTTCTGTTTTGCTAAGTCTT+TGG + Chr8:25389577-25389596 None:intergenic 30.0%
ACATATTGTGAGAGAAGAAC+AGG + Chr8:25389655-25389674 None:intergenic 35.0%
GTGAGAGAAGAACAGGAAAG+TGG + Chr8:25389648-25389667 None:intergenic 45.0%
! AAGTCTTTGGCTTCCTCGAT+AGG + Chr8:25389564-25389583 None:intergenic 45.0%
CTTCCTCGATAGGCACTTCT+CGG + Chr8:25389554-25389573 None:intergenic 50.0%
GCACTTCTCGGATTGAACTC+AGG + Chr8:25389542-25389561 None:intergenic 50.0%
!! AATCCGAGAAGTGCCTATCG+AGG - Chr8:25389548-25389567 MsG0880043479.01.T01:CDS 50.0%
Chromosome Type Strat End Strand Name
Chr8 gene 25389516 25389686 25389516 ID=MsG0880043479.01;Name=MsG0880043479.01
Chr8 mRNA 25389516 25389686 25389516 ID=MsG0880043479.01.T01;Parent=MsG0880043479.01;Name=MsG0880043479.01.T01;_AED=0.49;_eAED=0.49;_QI=0|-1|0|1|-1|0|1|0|56
Chr8 exon 25389516 25389686 25389516 ID=MsG0880043479.01.T01:exon:1154;Parent=MsG0880043479.01.T01
Chr8 CDS 25389516 25389686 25389516 ID=MsG0880043479.01.T01:cds;Parent=MsG0880043479.01.T01
Gene Sequence

>MsG0880043479.01.T01

ATGCTTGTTGGCAACAAGTCTGACCTGAGTTCAATCCGAGAAGTGCCTATCGAGGAAGCCAAAGACTTAGCAAAACAGAAAGGTCTCTACTTCATTGAGACATCAGCACTCGACTCAAGCAATGTAGAACCACTTTCCTGTTCTTCTCTCACAATATGTAACAATGAGTAA

Protein sequence

>MsG0880043479.01.T01

MLVGNKSDLSSIREVPIEEAKDLAKQKGLYFIETSALDSSNVEPLSCSSLTICNNE*