Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880046349.01.T01 | XP_003608943.1 | 95.395 | 152 | 4 | 1 | 1 | 152 | 1 | 149 | 7.47E-102 | 298 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180000139.01 | MsG0880046349.01 | -0.807766 | 4.306657e-50 | 2.419252e-47 |
| MsG0180004152.01 | MsG0880046349.01 | -0.805635 | 1.213673e-49 | 6.453670e-47 |
| MsG0180005962.01 | MsG0880046349.01 | 0.813650 | 2.299291e-51 | 1.510213e-48 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880046349.01.T01 | MTR_4g106650 | 95.395 | 152 | 4 | 1 | 1 | 152 | 1 | 149 | 9.05e-106 | 298 |
| MsG0880046349.01.T01 | MTR_3g078240 | 50.407 | 123 | 32 | 5 | 22 | 135 | 20 | 122 | 4.73e-31 | 109 |
| MsG0880046349.01.T01 | MTR_6g007727 | 42.857 | 126 | 57 | 6 | 17 | 137 | 25 | 140 | 8.85e-24 | 90.9 |
| MsG0880046349.01.T01 | MTR_2g461190 | 46.835 | 79 | 35 | 4 | 58 | 134 | 57 | 130 | 4.32e-13 | 62.8 |
| MsG0880046349.01.T01 | MTR_4g116530 | 28.889 | 135 | 88 | 4 | 1 | 133 | 9 | 137 | 5.06e-12 | 60.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880046349.01.T01 | AT5G62865 | 41.447 | 152 | 66 | 7 | 3 | 139 | 1 | 144 | 9.01e-19 | 78.6 |
| MsG0880046349.01.T01 | AT3G01430 | 34.194 | 155 | 59 | 6 | 14 | 137 | 21 | 163 | 1.08e-17 | 75.9 |
| MsG0880046349.01.T01 | AT3G48020 | 37.705 | 122 | 55 | 4 | 26 | 139 | 14 | 122 | 1.99e-16 | 71.6 |
| MsG0880046349.01.T01 | AT5G14895 | 35.252 | 139 | 60 | 7 | 17 | 137 | 24 | 150 | 3.40e-16 | 71.6 |
Find 58 sgRNAs with CRISPR-Local
Find 61 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCAGGCTCGGAGTTTCTTTA+AGG | 0.198226 | 8:-72288744 | None:intergenic |
| CCGGAACCGGAGGGTGAAAT+TGG | 0.303837 | 8:+72288968 | MsG0880046349.01.T01:CDS |
| CGAATGAAATTCTTCCATCT+TGG | 0.312826 | 8:-72288785 | None:intergenic |
| CCAATTTCACCCTCCGGTTC+CGG | 0.326252 | 8:-72288968 | None:intergenic |
| ATAGCCACGACGACGGGTTT+CGG | 0.342260 | 8:+72288900 | MsG0880046349.01.T01:CDS |
| TGGTTGTGGTGGTGGTGGGA+TGG | 0.347361 | 8:-72288688 | None:intergenic |
| GGCGGTGGTTGTGGTGGTGG+TGG | 0.369594 | 8:-72288693 | None:intergenic |
| GGTTGTGGTGGTGGTGGGAT+GGG | 0.371805 | 8:-72288687 | None:intergenic |
| CCATCTATCGGCGGTGGTTG+TGG | 0.375414 | 8:-72288702 | None:intergenic |
| TAAGTCTGTTGCGCCGGAAC+CGG | 0.391391 | 8:+72288955 | MsG0880046349.01.T01:CDS |
| CACGCCGCCGTTCCGTTTGG+TGG | 0.400297 | 8:+72288633 | MsG0880046349.01.T01:CDS |
| CCACAACCACCGCCGATAGA+TGG | 0.401522 | 8:+72288702 | MsG0880046349.01.T01:CDS |
| GTCTGTTGCGCCGGAACCGG+AGG | 0.417562 | 8:+72288958 | MsG0880046349.01.T01:CDS |
| ATCGGCGGTGGTTGTGGTGG+TGG | 0.420574 | 8:-72288696 | None:intergenic |
| CCTCACGCCGCCGTTCCGTT+TGG | 0.424502 | 8:+72288630 | MsG0880046349.01.T01:CDS |
| GCCTGGTCGGAGATCGTCGC+TGG | 0.451987 | 8:+72288761 | MsG0880046349.01.T01:CDS |
| CGCAACAGACTTAATATTAA+CGG | 0.461558 | 8:-72288945 | None:intergenic |
| CCTTAAAGAAACTCCGAGCC+TGG | 0.463778 | 8:+72288744 | MsG0880046349.01.T01:CDS |
| TTGTCATACGCTTCGATCGA+TGG | 0.470318 | 8:-72288820 | None:intergenic |
| ACGGCGGCGTGAGGCGAAGC+AGG | 0.472114 | 8:-72288621 | None:intergenic |
| ATGAAGATAGCCACGACGAC+GGG | 0.475007 | 8:+72288894 | MsG0880046349.01.T01:CDS |
| CAAGATGGAAGAATTTCATT+CGG | 0.477769 | 8:+72288786 | MsG0880046349.01.T01:CDS |
| GCGGTGGTTGTGGTGGTGGT+GGG | 0.485464 | 8:-72288692 | None:intergenic |
| CGGCGTGAGGCGAAGCAGGT+GGG | 0.494662 | 8:-72288617 | None:intergenic |
| GAAGCAGGTGGGGAAACATA+AGG | 0.500761 | 8:-72288606 | None:intergenic |
| AACCCGCCAATTTCACCCTC+CGG | 0.509850 | 8:-72288974 | None:intergenic |
| GGTGGGATGGGATTGAGAAA+AGG | 0.511993 | 8:-72288675 | None:intergenic |
| AACCGGAGGGTGAAATTGGC+GGG | 0.518811 | 8:+72288972 | MsG0880046349.01.T01:CDS |
| GAACCGGAGGGTGAAATTGG+CGG | 0.518878 | 8:+72288971 | MsG0880046349.01.T01:CDS |
| GGCGTGAGGCGAAGCAGGTG+GGG | 0.526513 | 8:-72288616 | None:intergenic |
| GCGGCGTGAGGCGAAGCAGG+TGG | 0.527709 | 8:-72288618 | None:intergenic |
| CGACGATCTCCGACCAGGCT+CGG | 0.535045 | 8:-72288757 | None:intergenic |
| ACGCCGCCGTTCCGTTTGGT+GGG | 0.538378 | 8:+72288634 | MsG0880046349.01.T01:CDS |
| TAATATTAAGTCTGTTGCGC+CGG | 0.541424 | 8:+72288949 | MsG0880046349.01.T01:CDS |
| ACCAGCGACGATCTCCGACC+AGG | 0.547492 | 8:-72288762 | None:intergenic |
| GCGAACACGTTCCCACCAAA+CGG | 0.551665 | 8:-72288645 | None:intergenic |
| AGATCGTCGCTGGTCCAAGA+TGG | 0.560579 | 8:+72288771 | MsG0880046349.01.T01:CDS |
| GCCGCGAGACCACCATCTAT+CGG | 0.563498 | 8:-72288714 | None:intergenic |
| CACGTTCCCACCAAACGGAA+CGG | 0.587139 | 8:-72288640 | None:intergenic |
| AGACCACCATCTATCGGCGG+TGG | 0.590655 | 8:-72288708 | None:intergenic |
| CCAAACGGAACGGCGGCGTG+AGG | 0.591562 | 8:-72288630 | None:intergenic |
| TCTATCGGCGGTGGTTGTGG+TGG | 0.591878 | 8:-72288699 | None:intergenic |
| TATGCGTTAAACTTTGATGA+GGG | 0.593416 | 8:+72288866 | MsG0880046349.01.T01:CDS |
| GGGATGGGATTGAGAAAAGG+AGG | 0.601763 | 8:-72288672 | None:intergenic |
| TTATGCGTTAAACTTTGATG+AGG | 0.610573 | 8:+72288865 | MsG0880046349.01.T01:CDS |
| GCCGATAGATGGTGGTCTCG+CGG | 0.626739 | 8:+72288713 | MsG0880046349.01.T01:CDS |
| TCTGTTGCGCCGGAACCGGA+GGG | 0.629319 | 8:+72288959 | MsG0880046349.01.T01:CDS |
| AATGAAGATAGCCACGACGA+CGG | 0.629695 | 8:+72288893 | MsG0880046349.01.T01:CDS |
| AATTCCGAAACCCGTCGTCG+TGG | 0.629946 | 8:-72288904 | None:intergenic |
| ATGGGATTGAGAAAAGGAGG+CGG | 0.635403 | 8:-72288669 | None:intergenic |
| AAAGAAACTCCGAGCCTGGT+CGG | 0.644913 | 8:+72288748 | MsG0880046349.01.T01:CDS |
| TGATTCAAGCTCTAGCTCAA+CGG | 0.647004 | 8:-72288549 | None:intergenic |
| AAGTTTAACGCATAACTCAA+CGG | 0.651799 | 8:-72288857 | None:intergenic |
| GGAGTTTCTTTAAGGCGTTG+AGG | 0.656051 | 8:-72288736 | None:intergenic |
| CAACCACCGCCGATAGATGG+TGG | 0.659317 | 8:+72288705 | MsG0880046349.01.T01:CDS |
| GTTCCCACCAAACGGAACGG+CGG | 0.663196 | 8:-72288637 | None:intergenic |
| AACAGACTTAATATTAACGG+CGG | 0.721385 | 8:-72288942 | None:intergenic |
| GCGAGACCACCATCTATCGG+CGG | 0.723063 | 8:-72288711 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AACAGACTTAATATTAACGG+CGG | - | Chr8:72288945-72288964 | None:intergenic | 30.0% | |
| AAGTTTAACGCATAACTCAA+CGG | - | Chr8:72288860-72288879 | None:intergenic | 30.0% | |
| CAAGATGGAAGAATTTCATT+CGG | + | Chr8:72288786-72288805 | MsG0880046349.01.T01:CDS | 30.0% | |
| CGCAACAGACTTAATATTAA+CGG | - | Chr8:72288948-72288967 | None:intergenic | 30.0% | |
| TATGCGTTAAACTTTGATGA+GGG | + | Chr8:72288866-72288885 | MsG0880046349.01.T01:CDS | 30.0% | |
| TTATGCGTTAAACTTTGATG+AGG | + | Chr8:72288865-72288884 | MsG0880046349.01.T01:CDS | 30.0% | |
| CGAATGAAATTCTTCCATCT+TGG | - | Chr8:72288788-72288807 | None:intergenic | 35.0% | |
| TAATATTAAGTCTGTTGCGC+CGG | + | Chr8:72288949-72288968 | MsG0880046349.01.T01:CDS | 35.0% | |
| ! | TCTCTTTTGTTGGAGATGAG+CGG | - | Chr8:72288579-72288598 | None:intergenic | 40.0% |
| ! | TGATTCAAGCTCTAGCTCAA+CGG | - | Chr8:72288552-72288571 | None:intergenic | 40.0% |
| AATGAAGATAGCCACGACGA+CGG | + | Chr8:72288893-72288912 | MsG0880046349.01.T01:CDS | 45.0% | |
| ATGGGATTGAGAAAAGGAGG+CGG | - | Chr8:72288672-72288691 | None:intergenic | 45.0% | |
| TTGTCATACGCTTCGATCGA+TGG | - | Chr8:72288823-72288842 | None:intergenic | 45.0% | |
| ! | AGGCGCTTGATCTCTTTTGT+TGG | - | Chr8:72288589-72288608 | None:intergenic | 45.0% |
| ! | TTTTGTTGGAGATGAGCGGT+TGG | - | Chr8:72288575-72288594 | None:intergenic | 45.0% |
| !! | GGAGTTTCTTTAAGGCGTTG+AGG | - | Chr8:72288739-72288758 | None:intergenic | 45.0% |
| AAAGAAACTCCGAGCCTGGT+CGG | + | Chr8:72288748-72288767 | MsG0880046349.01.T01:CDS | 50.0% | |
| ATGAAGATAGCCACGACGAC+GGG | + | Chr8:72288894-72288913 | MsG0880046349.01.T01:CDS | 50.0% | |
| CCAGGCTCGGAGTTTCTTTA+AGG | - | Chr8:72288747-72288766 | None:intergenic | 50.0% | |
| CCTTAAAGAAACTCCGAGCC+TGG | + | Chr8:72288744-72288763 | MsG0880046349.01.T01:CDS | 50.0% | |
| GAAGCAGGTGGGGAAACATA+AGG | - | Chr8:72288609-72288628 | None:intergenic | 50.0% | |
| GGGATGGGATTGAGAAAAGG+AGG | - | Chr8:72288675-72288694 | None:intergenic | 50.0% | |
| GGTGGGATGGGATTGAGAAA+AGG | - | Chr8:72288678-72288697 | None:intergenic | 50.0% | |
| AACCCGCCAATTTCACCCTC+CGG | - | Chr8:72288977-72288996 | None:intergenic | 55.0% | |
| AACCGGAGGGTGAAATTGGC+GGG | + | Chr8:72288972-72288991 | MsG0880046349.01.T01:CDS | 55.0% | |
| AATTCCGAAACCCGTCGTCG+TGG | - | Chr8:72288907-72288926 | None:intergenic | 55.0% | |
| AGATCGTCGCTGGTCCAAGA+TGG | + | Chr8:72288771-72288790 | MsG0880046349.01.T01:CDS | 55.0% | |
| CACGTTCCCACCAAACGGAA+CGG | - | Chr8:72288643-72288662 | None:intergenic | 55.0% | |
| CCAATTTCACCCTCCGGTTC+CGG | - | Chr8:72288971-72288990 | None:intergenic | 55.0% | |
| GAACCGGAGGGTGAAATTGG+CGG | + | Chr8:72288971-72288990 | MsG0880046349.01.T01:CDS | 55.0% | |
| GCGAACACGTTCCCACCAAA+CGG | - | Chr8:72288648-72288667 | None:intergenic | 55.0% | |
| TAAGTCTGTTGCGCCGGAAC+CGG | + | Chr8:72288955-72288974 | MsG0880046349.01.T01:CDS | 55.0% | |
| ! | ATAGCCACGACGACGGGTTT+CGG | + | Chr8:72288900-72288919 | MsG0880046349.01.T01:CDS | 55.0% |
| AGACCACCATCTATCGGCGG+TGG | - | Chr8:72288711-72288730 | None:intergenic | 60.0% | |
| CAACCACCGCCGATAGATGG+TGG | + | Chr8:72288705-72288724 | MsG0880046349.01.T01:CDS | 60.0% | |
| CCACAACCACCGCCGATAGA+TGG | + | Chr8:72288702-72288721 | MsG0880046349.01.T01:CDS | 60.0% | |
| CCATCTATCGGCGGTGGTTG+TGG | - | Chr8:72288705-72288724 | None:intergenic | 60.0% | |
| CCGGAACCGGAGGGTGAAAT+TGG | + | Chr8:72288968-72288987 | MsG0880046349.01.T01:CDS | 60.0% | |
| GCCGCGAGACCACCATCTAT+CGG | - | Chr8:72288717-72288736 | None:intergenic | 60.0% | |
| GCGAGACCACCATCTATCGG+CGG | - | Chr8:72288714-72288733 | None:intergenic | 60.0% | |
| GTTCCCACCAAACGGAACGG+CGG | - | Chr8:72288640-72288659 | None:intergenic | 60.0% | |
| TCTATCGGCGGTGGTTGTGG+TGG | - | Chr8:72288702-72288721 | None:intergenic | 60.0% | |
| ! | GCCGATAGATGGTGGTCTCG+CGG | + | Chr8:72288713-72288732 | MsG0880046349.01.T01:CDS | 60.0% |
| !! | GGTTGTGGTGGTGGTGGGAT+GGG | - | Chr8:72288690-72288709 | None:intergenic | 60.0% |
| !! | TGGTTGTGGTGGTGGTGGGA+TGG | - | Chr8:72288691-72288710 | None:intergenic | 60.0% |
| ACCAGCGACGATCTCCGACC+AGG | - | Chr8:72288765-72288784 | None:intergenic | 65.0% | |
| ACGCCGCCGTTCCGTTTGGT+GGG | + | Chr8:72288634-72288653 | MsG0880046349.01.T01:CDS | 65.0% | |
| ATCGGCGGTGGTTGTGGTGG+TGG | - | Chr8:72288699-72288718 | None:intergenic | 65.0% | |
| CGACGATCTCCGACCAGGCT+CGG | - | Chr8:72288760-72288779 | None:intergenic | 65.0% | |
| TCTGTTGCGCCGGAACCGGA+GGG | + | Chr8:72288959-72288978 | MsG0880046349.01.T01:CDS | 65.0% | |
| !! | GCGGTGGTTGTGGTGGTGGT+GGG | - | Chr8:72288695-72288714 | None:intergenic | 65.0% |
| CACGCCGCCGTTCCGTTTGG+TGG | + | Chr8:72288633-72288652 | MsG0880046349.01.T01:CDS | 70.0% | |
| CCAAACGGAACGGCGGCGTG+AGG | - | Chr8:72288633-72288652 | None:intergenic | 70.0% | |
| CCTCACGCCGCCGTTCCGTT+TGG | + | Chr8:72288630-72288649 | MsG0880046349.01.T01:CDS | 70.0% | |
| CGGCGTGAGGCGAAGCAGGT+GGG | - | Chr8:72288620-72288639 | None:intergenic | 70.0% | |
| GCCTGGTCGGAGATCGTCGC+TGG | + | Chr8:72288761-72288780 | MsG0880046349.01.T01:CDS | 70.0% | |
| GGCGTGAGGCGAAGCAGGTG+GGG | - | Chr8:72288619-72288638 | None:intergenic | 70.0% | |
| GTCTGTTGCGCCGGAACCGG+AGG | + | Chr8:72288958-72288977 | MsG0880046349.01.T01:CDS | 70.0% | |
| !! | GGCGGTGGTTGTGGTGGTGG+TGG | - | Chr8:72288696-72288715 | None:intergenic | 70.0% |
| ACGGCGGCGTGAGGCGAAGC+AGG | - | Chr8:72288624-72288643 | None:intergenic | 75.0% | |
| GCGGCGTGAGGCGAAGCAGG+TGG | - | Chr8:72288621-72288640 | None:intergenic | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr8 | gene | 72288545 | 72289003 | 72288545 | ID=MsG0880046349.01;Name=MsG0880046349.01 |
| Chr8 | mRNA | 72288545 | 72289003 | 72288545 | ID=MsG0880046349.01.T01;Parent=MsG0880046349.01;Name=MsG0880046349.01.T01;_AED=0.45;_eAED=0.45;_QI=0|-1|0|1|-1|1|1|0|152 |
| Chr8 | exon | 72288545 | 72289003 | 72288545 | ID=MsG0880046349.01.T01:exon:31134;Parent=MsG0880046349.01.T01 |
| Chr8 | CDS | 72288545 | 72289003 | 72288545 | ID=MsG0880046349.01.T01:cds;Parent=MsG0880046349.01.T01 |
| Gene Sequence |
| Protein sequence |
