AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0880046364.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880046364.01.T01 MTR_4g106860 99.130 115 1 0 1 115 1 115 1.70e-80 231
MsG0880046364.01.T01 MTR_8g062800 56.364 110 44 1 6 115 7 112 2.00e-38 125
MsG0880046364.01.T01 MTR_3g113890 47.826 115 48 3 1 115 1 103 5.97e-28 98.6
MsG0880046364.01.T01 MTR_1g025220 46.957 115 47 3 1 115 1 101 9.75e-27 95.5
MsG0880046364.01.T01 MTR_6g007897 72.131 61 17 0 55 115 188 248 6.89e-25 94.7
MsG0880046364.01.T01 MTR_1g025250 63.636 66 23 1 50 115 36 100 9.87e-23 85.1
MsG0880046364.01.T01 MTR_8g075920 62.121 66 24 1 50 115 39 103 1.54e-21 82.4
MsG0880046364.01.T01 MTR_1g096370 48.718 78 38 2 39 115 19 95 3.35e-19 76.3
MsG0880046364.01.T01 MTR_6g007913 59.677 62 24 1 55 115 77 138 5.41e-17 71.6
MsG0880046364.01.T01 MTR_6g007917 57.377 61 26 0 55 115 98 158 5.87e-17 72.0
MsG0880046364.01.T01 MTR_0254s0070 56.452 62 26 1 55 115 103 164 4.34e-14 64.7
MsG0880046364.01.T01 MTR_3g113850 59.091 44 18 0 55 98 406 449 7.13e-11 58.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880046364.01.T01 AT5G14920 66.129 62 21 0 54 115 214 275 2.04e-22 89.0
MsG0880046364.01.T01 AT4G09600 64.516 62 21 1 54 115 39 99 9.39e-22 82.8
MsG0880046364.01.T01 AT2G18420 62.903 62 22 1 54 115 34 94 7.99e-21 80.5
MsG0880046364.01.T01 AT2G18420 62.903 62 22 1 54 115 10 70 8.03e-21 79.7
MsG0880046364.01.T01 AT1G75750 57.576 66 27 1 50 115 34 98 1.09e-20 80.1
MsG0880046364.01.T01 AT1G75750 57.576 66 27 1 50 115 33 97 1.27e-20 79.7
MsG0880046364.01.T01 AT1G22690 58.065 62 25 1 54 115 59 119 5.99e-19 76.3
MsG0880046364.01.T01 AT1G22690 58.065 62 25 1 54 115 59 119 5.99e-19 76.3
MsG0880046364.01.T01 AT1G22690 58.065 62 25 1 54 115 59 119 5.99e-19 76.3
MsG0880046364.01.T01 AT4G09610 61.290 62 23 1 54 115 39 99 3.67e-13 60.8
MsG0880046364.01.T01 AT1G74670 31.858 113 65 2 3 115 1 101 2.43e-11 56.2

Find 51 sgRNAs with CRISPR-Local

Find 78 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGTTTAATATTGTTGATATT+TGG 0.162273 8:-72435471 MsG0880046364.01.T01:three_prime_UTR
CATGCTCTCTTGCACAAATT+TGG 0.199596 8:+72435854 None:intergenic
CATTGTTCTCTATAATTATT+AGG 0.211018 8:+72435391 None:intergenic
TACTAGTGTAGTTTGGTAAA+TGG 0.244704 8:-72435651 MsG0880046364.01.T01:three_prime_UTR
TTTAAAATTCTCCTCTCTAA+TGG 0.280437 8:-72436534 MsG0880046364.01.T01:exon
GCCCATGGGCCTAATTAGTT+TGG 0.284816 8:-72435700 MsG0880046364.01.T01:three_prime_UTR
GGGCTTTGATAAGGTCTCTA+AGG 0.305310 8:+72435719 None:intergenic
TTACGCAAGCTTCTTGTCTT+CGG 0.310911 8:-72436509 MsG0880046364.01.T01:CDS
GAAGAGAGCCCAAACTAATT+AGG 0.320634 8:+72435691 None:intergenic
TACGCAAGCTTCTTGTCTTC+GGG 0.326759 8:-72436508 MsG0880046364.01.T01:CDS
CACCTGGTACTGCCGGTAAT+AGG 0.335185 8:-72435799 MsG0880046364.01.T01:CDS
TATGTTGTGGTAACTAACTA+TGG 0.368562 8:-72435524 MsG0880046364.01.T01:three_prime_UTR
ACCTGGTACTGCCGGTAATA+GGG 0.401958 8:-72435798 MsG0880046364.01.T01:CDS
CCCATGGGCCTAATTAGTTT+GGG 0.408002 8:-72435699 MsG0880046364.01.T01:three_prime_UTR
AAACTATACTCATCAACTTA+AGG 0.422877 8:-72436577 None:intergenic
TCCACAAAACTCCCTATTAC+CGG 0.431014 8:+72435787 None:intergenic
ACTAGTGTAGTTTGGTAAAT+GGG 0.439046 8:-72435650 MsG0880046364.01.T01:three_prime_UTR
TAGGCCCATGGGCTTTGATA+AGG 0.442762 8:+72435710 None:intergenic
CAAGGTGCAGTGTGCATTCA+AGG 0.442762 8:-72435877 MsG0880046364.01.T01:CDS
CAGACTGTGGAGGGCTATGC+AGG 0.492139 8:-72435901 MsG0880046364.01.T01:CDS
TAGTACCTACTAGTGTAGTT+TGG 0.501441 8:-72435658 MsG0880046364.01.T01:three_prime_UTR
ATTTACCAAACTACACTAGT+AGG 0.502074 8:+72435653 None:intergenic
CTTGGTCTTGTTACCATGAG+TGG 0.515862 8:+72435745 None:intergenic
ATCTGATCATGAGATAGAAA+AGG 0.540603 8:-72436218 MsG0880046364.01.T01:CDS
TCCCTATTACCGGCAGTACC+AGG 0.543014 8:+72435797 None:intergenic
AATGCATTTAACTGATAAGC+CGG 0.550151 8:-72436182 MsG0880046364.01.T01:intron
GCCCAAACTAATTAGGCCCA+TGG 0.551582 8:+72435698 None:intergenic
ATAAGGTCTCTAAGGGCACT+TGG 0.552905 8:+72435727 None:intergenic
TGTGTCCCACCTGGTACTGC+CGG 0.553087 8:-72435806 MsG0880046364.01.T01:CDS
GGCTTTGATAAGGTCTCTAA+GGG 0.558100 8:+72435720 None:intergenic
CTTCAGCTTATTGTGCGAGA+CGG 0.558872 8:-72436046 MsG0880046364.01.T01:intron
AGAGACCTTATCAAAGCCCA+TGG 0.565394 8:-72435715 MsG0880046364.01.T01:three_prime_UTR
TACACTGATATGACCACTCA+TGG 0.567522 8:-72435758 MsG0880046364.01.T01:CDS
TGTTAAACCTTTGTATTCGT+AGG 0.570058 8:-72435571 MsG0880046364.01.T01:three_prime_UTR
CCCAAACTAATTAGGCCCAT+GGG 0.581285 8:+72435699 None:intergenic
AGAAGGCTAATGCAAGACAT+AGG 0.585779 8:-72436019 MsG0880046364.01.T01:intron
AGGTGCAAGTGTGTCCCACC+TGG 0.590839 8:-72435815 MsG0880046364.01.T01:CDS
AATTTGTGCAAGAGAGCATG+TGG 0.594899 8:-72435851 MsG0880046364.01.T01:CDS
GTGGAGGGCTATGCAGGTCA+AGG 0.600120 8:-72435895 MsG0880046364.01.T01:CDS
TTGTGCGAGACGGAAACAGA+AGG 0.605905 8:-72436036 MsG0880046364.01.T01:CDS
TCAAAATCCTACGAATACAA+AGG 0.641840 8:+72435564 None:intergenic
CATGTGGGACTTGCTGTGTG+AGG 0.647718 8:-72435835 MsG0880046364.01.T01:CDS
TACAAGTTAGTACCTTAGAG+AGG 0.650629 8:+72436468 None:intergenic
AGTCATATCTATCCGATGTG+AGG 0.655410 8:-72435425 MsG0880046364.01.T01:three_prime_UTR
CTTGCGTAATGCCATTAGAG+AGG 0.657310 8:+72436523 None:intergenic
GAGACCTTATCAAAGCCCAT+GGG 0.661282 8:-72435714 MsG0880046364.01.T01:three_prime_UTR
TACACTAGTAGGTACTACGT+GGG 0.679336 8:+72435664 None:intergenic
CTACACTAGTAGGTACTACG+TGG 0.684236 8:+72435663 None:intergenic
CTATTACCGGCAGTACCAGG+TGG 0.706350 8:+72435800 None:intergenic
TATTACCGGCAGTACCAGGT+GGG 0.719553 8:+72435801 None:intergenic
ATTTGTGCAAGAGAGCATGT+GGG 0.729137 8:-72435850 MsG0880046364.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTTGAATGAAAAAAAATAAA+AGG - Chr8:72436329-72436348 MsG0880046364.01.T01:intron 10.0%
!!! GTATATTTAGTTTTATTTTT+TGG + Chr8:72435959-72435978 None:intergenic 10.0%
!! ATAAGCATTTAACATATATT+AGG + Chr8:72435871-72435890 None:intergenic 15.0%
!! GTTCTATTTAAGTATAAAAA+AGG + Chr8:72435849-72435868 None:intergenic 15.0%
!! AGAAAGTAAAAAATGAATAC+CGG + Chr8:72435788-72435807 None:intergenic 20.0%
!! CATTGTTCTCTATAATTATT+AGG + Chr8:72436560-72436579 None:intergenic 20.0%
!! CGTTTAATATTGTTGATATT+TGG - Chr8:72436477-72436496 MsG0880046364.01.T01:intron 20.0%
!! GAATGAAAAAAAATAAAAGG+AGG - Chr8:72436332-72436351 MsG0880046364.01.T01:intron 20.0%
!! TTTCAACCAATAACTTAAAA+TGG + Chr8:72435588-72435607 None:intergenic 20.0%
!!! TTCATTTTTTACTTTCTTTC+TGG - Chr8:72435791-72435810 MsG0880046364.01.T01:CDS 20.0%
! AATGTTCAGTATAAGATGAA+AGG + Chr8:72435510-72435529 None:intergenic 25.0%
!! ATAAAAAAGGTGAAGTACTT+TGG + Chr8:72435836-72435855 None:intergenic 25.0%
!! TTTAAAATTCTCCTCTCTAA+TGG - Chr8:72435414-72435433 MsG0880046364.01.T01:three_prime_UTR 25.0%
!!! TTTGGACCATTTTAAGTTAT+TGG - Chr8:72435579-72435598 MsG0880046364.01.T01:three_prime_UTR 25.0%
AATGCATTTAACTGATAAGC+CGG - Chr8:72435766-72435785 MsG0880046364.01.T01:CDS 30.0%
ACTAGTGTAGTTTGGTAAAT+GGG - Chr8:72436298-72436317 MsG0880046364.01.T01:intron 30.0%
ATCTGATCATGAGATAGAAA+AGG - Chr8:72435730-72435749 MsG0880046364.01.T01:exon 30.0%
ATTTACCAAACTACACTAGT+AGG + Chr8:72436298-72436317 None:intergenic 30.0%
TACTAGTGTAGTTTGGTAAA+TGG - Chr8:72436297-72436316 MsG0880046364.01.T01:intron 30.0%
TATGTTGTGGTAACTAACTA+TGG - Chr8:72436424-72436443 MsG0880046364.01.T01:intron 30.0%
TCAAAATCCTACGAATACAA+AGG + Chr8:72436387-72436406 None:intergenic 30.0%
TGTTAAACCTTTGTATTCGT+AGG - Chr8:72436377-72436396 MsG0880046364.01.T01:intron 30.0%
!! GTAGGATTTTGATGTATTCT+CGG - Chr8:72436395-72436414 MsG0880046364.01.T01:intron 30.0%
GAAAAATACAAGCCTCACAT+CGG + Chr8:72436538-72436557 None:intergenic 35.0%
GTTCAGTATAAGATGAAAGG+TGG + Chr8:72435507-72435526 None:intergenic 35.0%
TACAAGTTAGTACCTTAGAG+AGG + Chr8:72435483-72435502 None:intergenic 35.0%
TAGTACCTACTAGTGTAGTT+TGG - Chr8:72436290-72436309 MsG0880046364.01.T01:intron 35.0%
! GTGTTTTGTCAATATCATGC+AGG - Chr8:72435703-72435722 MsG0880046364.01.T01:three_prime_UTR 35.0%
! TTCTCGGTTTTTGTATGTTG+TGG - Chr8:72436411-72436430 MsG0880046364.01.T01:intron 35.0%
AAGCCCTTGGTTTATGTCAT+GGG - Chr8:72435552-72435571 MsG0880046364.01.T01:three_prime_UTR 40.0%
AGAAGGCTAATGCAAGACAT+AGG - Chr8:72435929-72435948 MsG0880046364.01.T01:intron 40.0%
AGTCATATCTATCCGATGTG+AGG - Chr8:72436523-72436542 MsG0880046364.01.T01:exon 40.0%
CATGCTCTCTTGCACAAATT+TGG + Chr8:72436097-72436116 None:intergenic 40.0%
GAAGAGAGCCCAAACTAATT+AGG + Chr8:72436260-72436279 None:intergenic 40.0%
TACACTAGTAGGTACTACGT+GGG + Chr8:72436287-72436306 None:intergenic 40.0%
TACACTGATATGACCACTCA+TGG - Chr8:72436190-72436209 MsG0880046364.01.T01:CDS 40.0%
TCCACAAAACTCCCTATTAC+CGG + Chr8:72436164-72436183 None:intergenic 40.0%
TTACGCAAGCTTCTTGTCTT+CGG - Chr8:72435439-72435458 MsG0880046364.01.T01:three_prime_UTR 40.0%
! AATTTGTGCAAGAGAGCATG+TGG - Chr8:72436097-72436116 MsG0880046364.01.T01:intron 40.0%
! AATTTTGTGCAGACTGTGGA+GGG - Chr8:72436038-72436057 MsG0880046364.01.T01:CDS 40.0%
! ATTTGTGCAAGAGAGCATGT+GGG - Chr8:72436098-72436117 MsG0880046364.01.T01:intron 40.0%
! GATCAATTTTGTGCAGACTG+TGG - Chr8:72436034-72436053 MsG0880046364.01.T01:CDS 40.0%
! GGCTTTGATAAGGTCTCTAA+GGG + Chr8:72436231-72436250 None:intergenic 40.0%
!! GTTTATGTCATGGGGTGTTT+TGG - Chr8:72435561-72435580 MsG0880046364.01.T01:three_prime_UTR 40.0%
ACACCCCATGACATAAACCA+AGG + Chr8:72435559-72435578 None:intergenic 45.0%
AGAGACCTTATCAAAGCCCA+TGG - Chr8:72436233-72436252 MsG0880046364.01.T01:intron 45.0%
AGCCCTTGGTTTATGTCATG+GGG - Chr8:72435553-72435572 MsG0880046364.01.T01:three_prime_UTR 45.0%
CAAGCCCTTGGTTTATGTCA+TGG - Chr8:72435551-72435570 MsG0880046364.01.T01:three_prime_UTR 45.0%
CACCCCATGACATAAACCAA+GGG + Chr8:72435558-72435577 None:intergenic 45.0%
CCCAAACTAATTAGGCCCAT+GGG + Chr8:72436252-72436271 None:intergenic 45.0%
CTACACTAGTAGGTACTACG+TGG + Chr8:72436288-72436307 None:intergenic 45.0%
CTTCAGCTTATTGTGCGAGA+CGG - Chr8:72435902-72435921 MsG0880046364.01.T01:intron 45.0%
CTTGCGTAATGCCATTAGAG+AGG + Chr8:72435428-72435447 None:intergenic 45.0%
GAGACCTTATCAAAGCCCAT+GGG - Chr8:72436234-72436253 MsG0880046364.01.T01:intron 45.0%
TACGCAAGCTTCTTGTCTTC+GGG - Chr8:72435440-72435459 MsG0880046364.01.T01:three_prime_UTR 45.0%
! CAATTTTGTGCAGACTGTGG+AGG - Chr8:72436037-72436056 MsG0880046364.01.T01:CDS 45.0%
! CCCATGGGCCTAATTAGTTT+GGG - Chr8:72436249-72436268 MsG0880046364.01.T01:intron 45.0%
! GCTTTTAGTGTGCCTCTCTA+AGG - Chr8:72435468-72435487 MsG0880046364.01.T01:three_prime_UTR 45.0%
! GGGCTTTGATAAGGTCTCTA+AGG + Chr8:72436232-72436251 None:intergenic 45.0%
!! ATAAGGTCTCTAAGGGCACT+TGG + Chr8:72436224-72436243 None:intergenic 45.0%
!! CTTGGTCTTGTTACCATGAG+TGG + Chr8:72436206-72436225 None:intergenic 45.0%
ACCTGGTACTGCCGGTAATA+GGG - Chr8:72436150-72436169 MsG0880046364.01.T01:intron 50.0%
ACTGCTCTGTTTCAAGCCCT+TGG - Chr8:72435539-72435558 MsG0880046364.01.T01:three_prime_UTR 50.0%
CAAGGTGCAGTGTGCATTCA+AGG - Chr8:72436071-72436090 MsG0880046364.01.T01:intron 50.0%
GCCCAAACTAATTAGGCCCA+TGG + Chr8:72436253-72436272 None:intergenic 50.0%
GCCCATGGGCCTAATTAGTT+TGG - Chr8:72436248-72436267 MsG0880046364.01.T01:intron 50.0%
TATTACCGGCAGTACCAGGT+GGG + Chr8:72436150-72436169 None:intergenic 50.0%
TTGTGCGAGACGGAAACAGA+AGG - Chr8:72435912-72435931 MsG0880046364.01.T01:intron 50.0%
! GCCGGTAATAGGGAGTTTTG+TGG - Chr8:72436160-72436179 MsG0880046364.01.T01:intron 50.0%
! TAGGCCCATGGGCTTTGATA+AGG + Chr8:72436241-72436260 None:intergenic 50.0%
CACCTGGTACTGCCGGTAAT+AGG - Chr8:72436149-72436168 MsG0880046364.01.T01:intron 55.0%
CTATTACCGGCAGTACCAGG+TGG + Chr8:72436151-72436170 None:intergenic 55.0%
TCCCTATTACCGGCAGTACC+AGG + Chr8:72436154-72436173 None:intergenic 55.0%
!! CATGTGGGACTTGCTGTGTG+AGG - Chr8:72436113-72436132 MsG0880046364.01.T01:intron 55.0%
AGGTGCAAGTGTGTCCCACC+TGG - Chr8:72436133-72436152 MsG0880046364.01.T01:intron 60.0%
CAGACTGTGGAGGGCTATGC+AGG - Chr8:72436047-72436066 MsG0880046364.01.T01:intron 60.0%
GTGGAGGGCTATGCAGGTCA+AGG - Chr8:72436053-72436072 MsG0880046364.01.T01:intron 60.0%
TGTGTCCCACCTGGTACTGC+CGG - Chr8:72436142-72436161 MsG0880046364.01.T01:intron 60.0%
Chromosome Type Strat End Strand Name
Chr8 gene 72435376 72436594 72435376 ID=MsG0880046364.01;Name=MsG0880046364.01
Chr8 mRNA 72435376 72436594 72435376 ID=MsG0880046364.01.T01;Parent=MsG0880046364.01;Name=MsG0880046364.01.T01;_AED=0.49;_eAED=0.49;_QI=57|1|1|1|0.66|0.75|4|360|115
Chr8 exon 72435376 72435920 72435376 ID=MsG0880046364.01.T01:exon:7912;Parent=MsG0880046364.01.T01
Chr8 exon 72436020 72436062 72436020 ID=MsG0880046364.01.T01:exon:7911;Parent=MsG0880046364.01.T01
Chr8 exon 72436183 72436245 72436183 ID=MsG0880046364.01.T01:exon:7910;Parent=MsG0880046364.01.T01
Chr8 exon 72436481 72436594 72436481 ID=MsG0880046364.01.T01:exon:7909;Parent=MsG0880046364.01.T01
Chr8 five_prime_UTR 72436538 72436594 72436538 ID=MsG0880046364.01.T01:five_prime_utr;Parent=MsG0880046364.01.T01
Chr8 CDS 72436481 72436537 72436481 ID=MsG0880046364.01.T01:cds;Parent=MsG0880046364.01.T01
Chr8 CDS 72436183 72436245 72436183 ID=MsG0880046364.01.T01:cds;Parent=MsG0880046364.01.T01
Chr8 CDS 72436020 72436062 72436020 ID=MsG0880046364.01.T01:cds;Parent=MsG0880046364.01.T01
Chr8 CDS 72435736 72435920 72435736 ID=MsG0880046364.01.T01:cds;Parent=MsG0880046364.01.T01
Chr8 three_prime_UTR 72435376 72435735 72435376 ID=MsG0880046364.01.T01:three_prime_utr;Parent=MsG0880046364.01.T01
Gene Sequence

>MsG0880046364.01.T01

ATGGCATTACGCAAGCTTCTTGTCTTCGGGTTGTTGCTTTTAGTGTGCCTCTCTAAGGTTTCATCTGATCATGAGATAGAAAAGGAAGAAGATGAAGAAATGCATTTAACTGATAAGCCGCTTATTGTGCGAGACGGAAACAGAAGGCTAATGCAAGACATAGACTGTGGAGGGCTATGCAGGTCAAGGTGCAGTGTGCATTCAAGGCCAAATTTGTGCAAGAGAGCATGTGGGACTTGCTGTGTGAGGTGCAAGTGTGTCCCACCTGGTACTGCCGGTAATAGGGAGTTTTGTGGAGCTTGTTACACTGATATGACCACTCATGGTAACAAGACCAAGTGCCCTTAG

Protein sequence

>MsG0880046364.01.T01

MALRKLLVFGLLLLVCLSKVSSDHEIEKEEDEEMHLTDKPLIVRDGNRRLMQDIDCGGLCRSRCSVHSRPNLCKRACGTCCVRCKCVPPGTAGNREFCGACYTDMTTHGNKTKCP*