AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0880046568.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880046568.01.T01 MTR_2g013980 88.298 94 11 0 1 94 1091 1184 3.06e-41 149
MsG0880046568.01.T01 MTR_5g037350 90.411 73 7 0 22 94 484 556 1.66e-37 137
MsG0880046568.01.T01 MTR_3g102480 92.188 64 5 0 1 64 1 64 3.96e-35 121
MsG0880046568.01.T01 MTR_5g024920 68.182 88 26 1 7 94 1 86 2.92e-29 115
MsG0880046568.01.T01 MTR_1g090220 61.053 95 25 1 2 84 83 177 3.11e-25 97.8
MsG0880046568.01.T01 MTR_1g021865 53.398 103 38 3 1 100 108 203 4.26e-25 98.2
MsG0880046568.01.T01 MTR_7g025530 69.412 85 25 1 10 94 240 323 4.82e-24 100
MsG0880046568.01.T01 MTR_3g467660 71.429 63 18 0 23 85 5 67 5.98e-23 94.7
MsG0880046568.01.T01 MTR_4g112860 85.965 57 7 1 129 184 571 627 9.98e-22 93.6
MsG0880046568.01.T01 MTR_6g086840 75.000 64 13 2 46 106 9 72 1.18e-21 87.0
MsG0880046568.01.T01 MTR_5g034890 50.588 85 17 1 10 94 166 225 7.20e-17 79.3
MsG0880046568.01.T01 MTR_2g058970 62.687 67 24 1 129 194 583 649 1.13e-15 75.9
MsG0880046568.01.T01 MTR_3g073740 89.189 37 4 0 50 86 10 46 2.57e-13 64.3
MsG0880046568.01.T01 MTR_5g031630 90.909 33 3 0 53 85 199 231 1.38e-11 63.5
MsG0880046568.01.T01 MTR_3g011720 68.889 45 13 1 23 67 31 74 6.33e-11 57.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880046568.01.T01 AT1G73990 43.011 93 51 2 94 184 553 645 1.94e-12 66.2

Find 76 sgRNAs with CRISPR-Local

Find 142 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCTCTATCTAATACGTATT+TGG 0.125015 8:-74963213 MsG0880046568.01.T01:three_prime_UTR
GTTCTATCAAAGACCATATT+TGG 0.156995 8:+74964457 None:intergenic
TGGAATCATGTTTCAGAAAT+TGG 0.170138 8:-74963416 MsG0880046568.01.T01:CDS
ATCCATCTGCTAACCCATTT+TGG 0.191467 8:-74963387 MsG0880046568.01.T01:CDS
ATCTTTCATCAATGCCAAAA+TGG 0.244554 8:+74963373 None:intergenic
TAGAAAAGTTGGTAGAAAAT+AGG 0.288105 8:-74964636 MsG0880046568.01.T01:CDS
CATGGTTTGGTTGATGCTAT+TGG 0.308966 8:-74963743 MsG0880046568.01.T01:CDS
GAAAATAGGCTTAGATGGTT+TGG 0.322680 8:-74964622 MsG0880046568.01.T01:CDS
CAAGATACTCCGCTCTTGTT+TGG 0.335632 8:-74963110 MsG0880046568.01.T01:three_prime_UTR
TCTTCTTACCACGGCGTCTA+CGG 0.338293 8:+74964582 None:intergenic
TGGTAGAAAATAGGCTTAGA+TGG 0.349489 8:-74964627 MsG0880046568.01.T01:CDS
ATGGAATGCTGCATCCTTAC+CGG 0.363453 8:+74963763 None:intergenic
AGGTTGCACAGGGGAGGGTT+TGG 0.365062 8:-74963787 MsG0880046568.01.T01:CDS
AACCATTAGAAAAGATTTAG+AGG 0.369865 8:-74964494 MsG0880046568.01.T01:CDS
CAATGAGTTGGATCCAAATA+TGG 0.382094 8:-74964470 MsG0880046568.01.T01:intron
AGATCAAATGGAGGAGAGTC+AGG 0.409734 8:-74964557 MsG0880046568.01.T01:CDS
AGACTAGACATGATAGGATT+AGG 0.412117 8:-74964699 MsG0880046568.01.T01:CDS
GGATGCAGCATTCCATGGTT+TGG 0.412526 8:-74963756 MsG0880046568.01.T01:CDS
AGTCAGGTTAAAAGAGGTAG+AGG 0.455911 8:-74964541 MsG0880046568.01.T01:CDS
AAGAGCGTCTGGTAATTCTC+TGG 0.457944 8:-74963511 MsG0880046568.01.T01:CDS
TCTTTCATCAATGCCAAAAT+GGG 0.460664 8:+74963374 None:intergenic
CTTCTTACCACGGCGTCTAC+GGG 0.468664 8:+74964583 None:intergenic
AAATCACTCTCTTTATTCCA+TGG 0.474625 8:+74963285 None:intergenic
TTGAGAAATTTGTATTCTGA+AGG 0.482578 8:-74963172 MsG0880046568.01.T01:three_prime_UTR
CTGATGGAGTTCAGCGCGAA+TGG 0.488153 8:-74963440 MsG0880046568.01.T01:CDS
TATAATAATAGAAGATTCCA+TGG 0.489365 8:-74963302 MsG0880046568.01.T01:exon
GGTAAGAAGAGTAGATCAAA+TGG 0.497053 8:-74964569 MsG0880046568.01.T01:CDS
ACCAGACGCTCTTAAAATCT+CGG 0.497808 8:+74963521 None:intergenic
AGATTTAGAGGTCAATGAGT+TGG 0.506040 8:-74964482 MsG0880046568.01.T01:CDS
GGAAGAGGTTGCACAGGGGA+GGG 0.508226 8:-74963792 MsG0880046568.01.T01:CDS
GAGAAATCAGATTTCTCAGT+GGG 0.513489 8:+74963231 None:intergenic
GAGGAGAGTCAGGTTAAAAG+AGG 0.517218 8:-74964547 MsG0880046568.01.T01:CDS
GCTCTTAAAATCTCGGTAAG+AGG 0.520838 8:+74963528 None:intergenic
ACAGATGGAAGAGGTTGCAC+AGG 0.522289 8:-74963798 MsG0880046568.01.T01:CDS
AGCACCTATAGTAGAAAAGT+TGG 0.529249 8:-74964647 MsG0880046568.01.T01:CDS
ATCATGTTTCAGAAATTGGA+AGG 0.534579 8:-74963412 MsG0880046568.01.T01:CDS
TCAATCACCTGTCTGTCTTG+AGG 0.535578 8:+74963676 None:intergenic
TGGAGTTCAGCGCGAATGGA+TGG 0.537309 8:-74963436 MsG0880046568.01.T01:CDS
TGGAAGAGGTTGCACAGGGG+AGG 0.540501 8:-74963793 MsG0880046568.01.T01:CDS
GGTAAGGATGCAGCATTCCA+TGG 0.553551 8:-74963761 MsG0880046568.01.T01:CDS
AAGAAGAGTAGATCAAATGG+AGG 0.556047 8:-74964566 MsG0880046568.01.T01:CDS
GGGGAGGGTTTGGACCGGTA+AGG 0.556238 8:-74963777 MsG0880046568.01.T01:CDS
GGTTTGGTTGATGCTATTGG+TGG 0.557324 8:-74963740 MsG0880046568.01.T01:CDS
TGCCAAAATGGGTTAGCAGA+TGG 0.565113 8:+74963385 None:intergenic
CAGATGGAAGAGGTTGCACA+GGG 0.565222 8:-74963797 MsG0880046568.01.T01:CDS
TGACATCATTAGAGAGAGAG+TGG 0.565974 8:-74964674 MsG0880046568.01.T01:CDS
TAATATACCTCAAGACAGAC+AGG 0.566826 8:-74963683 MsG0880046568.01.T01:intron
TCTATGCCAGGTTACTGTTG+TGG 0.567041 8:-74963568 MsG0880046568.01.T01:intron
AGGATGTTGCGTTGGATGAG+TGG 0.569707 8:-74964724 MsG0880046568.01.T01:CDS
AAAATCTCGGTAAGAGGACG+AGG 0.572177 8:+74963534 None:intergenic
TCAGTTCTACTACATAGAGA+GGG 0.574362 8:-74963346 MsG0880046568.01.T01:CDS
GCGTCTGGTAATTCTCTGGT+TGG 0.580134 8:-74963507 MsG0880046568.01.T01:CDS
CGGAGATGAGGATGTTGCGT+TGG 0.581910 8:-74964732 None:intergenic
GACATCATTAGAGAGAGAGT+GGG 0.583558 8:-74964673 MsG0880046568.01.T01:CDS
TGGTAATTCTCTGGTTGGAG+TGG 0.583715 8:-74963502 MsG0880046568.01.T01:CDS
TTGATCTACTCTTCTTACCA+CGG 0.584343 8:+74964573 None:intergenic
GTGGTAAGACTAGACATGAT+AGG 0.588491 8:-74964705 MsG0880046568.01.T01:CDS
TTCTCAACATTATGACAACA+TGG 0.590336 8:+74963188 None:intergenic
GTTTGGTTGATGCTATTGGT+GGG 0.590597 8:-74963739 MsG0880046568.01.T01:CDS
GCACAGGGGAGGGTTTGGAC+CGG 0.592535 8:-74963782 MsG0880046568.01.T01:CDS
TCCGTTGCTAGGTAGACAGA+TGG 0.593980 8:-74963813 MsG0880046568.01.T01:intron
CTCAGTTCTACTACATAGAG+AGG 0.600032 8:-74963347 MsG0880046568.01.T01:CDS
AACACTCAAGGAACTACTAG+AGG 0.611061 8:-74963475 MsG0880046568.01.T01:CDS
TGGAGTGGACGAAACACTCA+AGG 0.619380 8:-74963487 MsG0880046568.01.T01:CDS
GAGAAGACCCGTAGACGCCG+TGG 0.623053 8:-74964590 MsG0880046568.01.T01:CDS
GCTAGGTAGACAGATGGAAG+AGG 0.628285 8:-74963807 MsG0880046568.01.T01:intron
ACATCATTAGAGAGAGAGTG+GGG 0.637541 8:-74964672 MsG0880046568.01.T01:CDS
CAATAGCATCAACCAAACCA+TGG 0.645415 8:+74963744 None:intergenic
CTCTCTATGTAGTAGAACTG+AGG 0.653257 8:+74963348 None:intergenic
CAGTTCTACTACATAGAGAG+GGG 0.669323 8:-74963345 MsG0880046568.01.T01:CDS
AGTTCTACTACATAGAGAGG+GGG 0.672431 8:-74963344 MsG0880046568.01.T01:CDS
CATCATTAGAGAGAGAGTGG+GGG 0.691334 8:-74964671 MsG0880046568.01.T01:CDS
GTTCTACTACATAGAGAGGG+GGG 0.691617 8:-74963343 MsG0880046568.01.T01:CDS
AACGTGAATCCAAACAAGAG+CGG 0.696169 8:+74963101 None:intergenic
AGAGAAATCAGATTTCTCAG+TGG 0.727390 8:+74963230 None:intergenic
AGATGGAAGAGGTTGCACAG+GGG 0.760558 8:-74963796 MsG0880046568.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATCATAAATCAGAGAAAA+AGG + Chr8:74963726-74963745 None:intergenic 20.0%
!! AATATCACAACTATAAAACA+TGG + Chr8:74963618-74963637 None:intergenic 20.0%
!! ATCAAAAATATAAACTGATG+CGG + Chr8:74963794-74963813 None:intergenic 20.0%
!! TATAAAACAATTTCGAGATA+AGG - Chr8:74963902-74963921 MsG0880046568.01.T01:intron 20.0%
!! TATAATAATAGAAGATTCCA+TGG - Chr8:74964533-74964552 MsG0880046568.01.T01:CDS 20.0%
!!! GTAGATTTTATTTTGATCTT+TGG - Chr8:74963494-74963513 MsG0880046568.01.T01:CDS 20.0%
!!! GTTTATATTTTTGATTTCCA+AGG - Chr8:74963799-74963818 MsG0880046568.01.T01:CDS 20.0%
!!! TAAAAACTTTATTTGTGTAG+AGG + Chr8:74964705-74964724 None:intergenic 20.0%
!!! TGTTTTATAGTTGTGATATT+TGG - Chr8:74963618-74963637 MsG0880046568.01.T01:intron 20.0%
! AACAAACAGTATCAAGAATA+AGG + Chr8:74964191-74964210 None:intergenic 25.0%
! AACCATTAGAAAAGATTTAG+AGG - Chr8:74963341-74963360 MsG0880046568.01.T01:CDS 25.0%
! AATTGTGTCATGAAAAAGAT+TGG + Chr8:74963677-74963696 None:intergenic 25.0%
! ACAAACAGTATCAAGAATAA+GGG + Chr8:74964190-74964209 None:intergenic 25.0%
! ATTGTGTCATGAAAAAGATT+GGG + Chr8:74963676-74963695 None:intergenic 25.0%
! TTCTCTATCTAATACGTATT+TGG - Chr8:74964622-74964641 MsG0880046568.01.T01:CDS 25.0%
! TTGAGAAATTTGTATTCTGA+AGG - Chr8:74964663-74964682 MsG0880046568.01.T01:CDS 25.0%
!! AATTTCTACGATTGATTCAT+GGG - Chr8:74963746-74963765 MsG0880046568.01.T01:CDS 25.0%
!! GTTTCTCTAATAGTTTTCTT+AGG + Chr8:74963324-74963343 None:intergenic 25.0%
!! TAATTTCTACGATTGATTCA+TGG - Chr8:74963745-74963764 MsG0880046568.01.T01:CDS 25.0%
!! TTATAAATTTTGTGCAGACT+TGG + Chr8:74963887-74963906 None:intergenic 25.0%
!!! TAGAAAAGTTGGTAGAAAAT+AGG - Chr8:74963199-74963218 MsG0880046568.01.T01:three_prime_UTR 25.0%
AAAACAATTTCGAGATAAGG+CGG - Chr8:74963905-74963924 MsG0880046568.01.T01:intron 30.0%
AAATCACTCTCTTTATTCCA+TGG + Chr8:74964553-74964572 None:intergenic 30.0%
ATCATGTTTCAGAAATTGGA+AGG - Chr8:74964423-74964442 MsG0880046568.01.T01:intron 30.0%
ATCTTTCATCAATGCCAAAA+TGG + Chr8:74964465-74964484 None:intergenic 30.0%
GTTCTATCAAAGACCATATT+TGG + Chr8:74963381-74963400 None:intergenic 30.0%
TAAAACATACAGTCATTGAC+TGG + Chr8:74963937-74963956 None:intergenic 30.0%
TAAACTGATGCGGAAAAAAA+TGG + Chr8:74963784-74963803 None:intergenic 30.0%
TCTTTCATCAATGCCAAAAT+GGG + Chr8:74964464-74964483 None:intergenic 30.0%
TCTTTGATAGAACATTATGG+CGG - Chr8:74963388-74963407 MsG0880046568.01.T01:CDS 30.0%
TGGAATCATGTTTCAGAAAT+TGG - Chr8:74964419-74964438 MsG0880046568.01.T01:intron 30.0%
TGGTCTTTGATAGAACATTA+TGG - Chr8:74963385-74963404 MsG0880046568.01.T01:CDS 30.0%
TTCTCAACATTATGACAACA+TGG + Chr8:74964650-74964669 None:intergenic 30.0%
! GACCTCTAAATCTTTTCTAA+TGG + Chr8:74963346-74963365 None:intergenic 30.0%
! TCTACCAACTTTTCTACTAT+AGG + Chr8:74963195-74963214 None:intergenic 30.0%
AAAGGCAACACTAATGACAA+AGG + Chr8:74963708-74963727 None:intergenic 35.0%
AAGAAGAGTAGATCAAATGG+AGG - Chr8:74963269-74963288 MsG0880046568.01.T01:three_prime_UTR 35.0%
AGACTAGACATGATAGGATT+AGG - Chr8:74963136-74963155 MsG0880046568.01.T01:three_prime_UTR 35.0%
AGAGAAATCAGATTTCTCAG+TGG + Chr8:74964608-74964627 None:intergenic 35.0%
AGATTTAGAGGTCAATGAGT+TGG - Chr8:74963353-74963372 MsG0880046568.01.T01:CDS 35.0%
AGCACCTATAGTAGAAAAGT+TGG - Chr8:74963188-74963207 MsG0880046568.01.T01:three_prime_UTR 35.0%
ATACCTTATGATTCTATGCC+AGG - Chr8:74964255-74964274 MsG0880046568.01.T01:intron 35.0%
CTGTAACTATTGGACATAGA+AGG + Chr8:74963996-74964015 None:intergenic 35.0%
GAAAATAGGCTTAGATGGTT+TGG - Chr8:74963213-74963232 MsG0880046568.01.T01:three_prime_UTR 35.0%
GAGAAATCAGATTTCTCAGT+GGG + Chr8:74964607-74964626 None:intergenic 35.0%
GGTAAGAAGAGTAGATCAAA+TGG - Chr8:74963266-74963285 MsG0880046568.01.T01:three_prime_UTR 35.0%
TAACCTGGCATAGAATCATA+AGG + Chr8:74964261-74964280 None:intergenic 35.0%
TAACTATTGGACATAGAAGG+AGG + Chr8:74963993-74964012 None:intergenic 35.0%
TAATATACCTCAAGACAGAC+AGG - Chr8:74964152-74964171 MsG0880046568.01.T01:intron 35.0%
TACTAGTCATCACCTTAAGT+AGG - Chr8:74963559-74963578 MsG0880046568.01.T01:CDS 35.0%
TCAGTTCTACTACATAGAGA+GGG - Chr8:74964489-74964508 MsG0880046568.01.T01:CDS 35.0%
TGGTAGAAAATAGGCTTAGA+TGG - Chr8:74963208-74963227 MsG0880046568.01.T01:three_prime_UTR 35.0%
TTGATCTACTCTTCTTACCA+CGG + Chr8:74963265-74963284 None:intergenic 35.0%
! CAATGAGTTGGATCCAAATA+TGG - Chr8:74963365-74963384 MsG0880046568.01.T01:CDS 35.0%
AACACTCAAGGAACTACTAG+AGG - Chr8:74964360-74964379 MsG0880046568.01.T01:intron 40.0%
ACATCATTAGAGAGAGAGTG+GGG - Chr8:74963163-74963182 MsG0880046568.01.T01:three_prime_UTR 40.0%
ACCAGACGCTCTTAAAATCT+CGG + Chr8:74964317-74964336 None:intergenic 40.0%
AGTCAGGTTAAAAGAGGTAG+AGG - Chr8:74963294-74963313 MsG0880046568.01.T01:three_prime_UTR 40.0%
AGTTCTACTACATAGAGAGG+GGG - Chr8:74964491-74964510 MsG0880046568.01.T01:CDS 40.0%
ATATTCCTACACTGTAAGCC+CGG + Chr8:74963587-74963606 None:intergenic 40.0%
CAATAGCATCAACCAAACCA+TGG + Chr8:74964094-74964113 None:intergenic 40.0%
CAGTTCTACTACATAGAGAG+GGG - Chr8:74964490-74964509 MsG0880046568.01.T01:CDS 40.0%
CGGATAGTAGCTGTAACTAT+TGG + Chr8:74964006-74964025 None:intergenic 40.0%
CTAGTAGTGCCTTATACTGA+TGG + Chr8:74963545-74963564 None:intergenic 40.0%
CTCAGTTCTACTACATAGAG+AGG - Chr8:74964488-74964507 MsG0880046568.01.T01:CDS 40.0%
CTCTCTATGTAGTAGAACTG+AGG + Chr8:74964490-74964509 None:intergenic 40.0%
GACATCATTAGAGAGAGAGT+GGG - Chr8:74963162-74963181 MsG0880046568.01.T01:three_prime_UTR 40.0%
GAGAATATATGTTGCTGCCT+TGG + Chr8:74963819-74963838 None:intergenic 40.0%
GCTCTTAAAATCTCGGTAAG+AGG + Chr8:74964310-74964329 None:intergenic 40.0%
GTGGTAAGACTAGACATGAT+AGG - Chr8:74963130-74963149 MsG0880046568.01.T01:three_prime_UTR 40.0%
TGACATCATTAGAGAGAGAG+TGG - Chr8:74963161-74963180 MsG0880046568.01.T01:three_prime_UTR 40.0%
! ATCCATCTGCTAACCCATTT+TGG - Chr8:74964448-74964467 MsG0880046568.01.T01:intron 40.0%
! CTACGATTGATTCATGGGTT+CGG - Chr8:74963751-74963770 MsG0880046568.01.T01:CDS 40.0%
! GAGGACTTGACATTTTCTGA+TGG - Chr8:74964379-74964398 MsG0880046568.01.T01:intron 40.0%
! GTTTGGTTGATGCTATTGGT+GGG - Chr8:74964096-74964115 MsG0880046568.01.T01:intron 40.0%
! TACGATTGATTCATGGGTTC+GGG - Chr8:74963752-74963771 MsG0880046568.01.T01:CDS 40.0%
!! CATGGTTTGGTTGATGCTAT+TGG - Chr8:74964092-74964111 MsG0880046568.01.T01:intron 40.0%
AAAATCTCGGTAAGAGGACG+AGG + Chr8:74964304-74964323 None:intergenic 45.0%
AACGCCTTATCCCACTAAGT+GGG + Chr8:74963435-74963454 None:intergenic 45.0%
ACCTCATATATACCTCGGGT+GGG - Chr8:74963649-74963668 MsG0880046568.01.T01:intron 45.0%
AGATCAAATGGAGGAGAGTC+AGG - Chr8:74963278-74963297 MsG0880046568.01.T01:three_prime_UTR 45.0%
ATGGAATGCTGCATCCTTAC+CGG + Chr8:74964075-74964094 None:intergenic 45.0%
CAAGATACTCCGCTCTTGTT+TGG - Chr8:74964725-74964744 MsG0880046568.01.T01:CDS 45.0%
CACCACCTCATATATACCTC+GGG - Chr8:74963645-74963664 MsG0880046568.01.T01:intron 45.0%
CATCATTAGAGAGAGAGTGG+GGG - Chr8:74963164-74963183 MsG0880046568.01.T01:three_prime_UTR 45.0%
CCACCACCTCATATATACCT+CGG - Chr8:74963644-74963663 MsG0880046568.01.T01:intron 45.0%
GAGGAGAGTCAGGTTAAAAG+AGG - Chr8:74963288-74963307 MsG0880046568.01.T01:three_prime_UTR 45.0%
GTTCTACTACATAGAGAGGG+GGG - Chr8:74964492-74964511 MsG0880046568.01.T01:CDS 45.0%
TACAGCTACTATCCGTTGCT+AGG - Chr8:74964011-74964030 MsG0880046568.01.T01:intron 45.0%
TACCCTCAGCCATCAGTATA+AGG - Chr8:74963533-74963552 MsG0880046568.01.T01:CDS 45.0%
TCAATCACCTGTCTGTCTTG+AGG + Chr8:74964162-74964181 None:intergenic 45.0%
TCCATCTGTCTACCTAGCAA+CGG + Chr8:74964026-74964045 None:intergenic 45.0%
TCTATGCCAGGTTACTGTTG+TGG - Chr8:74964267-74964286 MsG0880046568.01.T01:intron 45.0%
TCTCTCTCTCTCTCAAGTAC+AGG - Chr8:74963839-74963858 MsG0880046568.01.T01:intron 45.0%
TGCCTTATACTGATGGCTGA+GGG + Chr8:74963538-74963557 None:intergenic 45.0%
TGGTAATTCTCTGGTTGGAG+TGG - Chr8:74964333-74964352 MsG0880046568.01.T01:intron 45.0%
! ATTGATTCATGGGTTCGGGT+TGG - Chr8:74963756-74963775 MsG0880046568.01.T01:CDS 45.0%
! GCAAATAGCTCTGCTTCATG+TGG + Chr8:74963865-74963884 None:intergenic 45.0%
! GGTTTGGTTGATGCTATTGG+TGG - Chr8:74964095-74964114 MsG0880046568.01.T01:intron 45.0%
!! AAGAGCGTCTGGTAATTCTC+TGG - Chr8:74964324-74964343 MsG0880046568.01.T01:intron 45.0%
!! TGCCAAAATGGGTTAGCAGA+TGG + Chr8:74964453-74964472 None:intergenic 45.0%
!!! ACCGAGATTTTAAGAGCGTC+TGG - Chr8:74964313-74964332 MsG0880046568.01.T01:intron 45.0%
ACAGATGGAAGAGGTTGCAC+AGG - Chr8:74964037-74964056 MsG0880046568.01.T01:intron 50.0%
ACCCACCCGAGGTATATATG+AGG + Chr8:74963653-74963672 None:intergenic 50.0%
ACGCCTTATCCCACTAAGTG+GGG + Chr8:74963434-74963453 None:intergenic 50.0%
AGAGCTCCACAACAGTAACC+TGG + Chr8:74964276-74964295 None:intergenic 50.0%
AGATGGAAGAGGTTGCACAG+GGG - Chr8:74964039-74964058 MsG0880046568.01.T01:intron 50.0%
AGGATGTTGCGTTGGATGAG+TGG - Chr8:74963111-74963130 MsG0880046568.01.T01:three_prime_UTR 50.0%
CAACGCCTTATCCCACTAAG+TGG + Chr8:74963436-74963455 None:intergenic 50.0%
CACCCGAGGTATATATGAGG+TGG + Chr8:74963650-74963669 None:intergenic 50.0%
CACCTCATATATACCTCGGG+TGG - Chr8:74963648-74963667 MsG0880046568.01.T01:intron 50.0%
CAGATGGAAGAGGTTGCACA+GGG - Chr8:74964038-74964057 MsG0880046568.01.T01:intron 50.0%
CCGAGGTATATATGAGGTGG+TGG + Chr8:74963647-74963666 None:intergenic 50.0%
CTTATCCCACTAAGTGGGGT+CGG + Chr8:74963430-74963449 None:intergenic 50.0%
GCGTCTGGTAATTCTCTGGT+TGG - Chr8:74964328-74964347 MsG0880046568.01.T01:intron 50.0%
GCTAGGTAGACAGATGGAAG+AGG - Chr8:74964028-74964047 MsG0880046568.01.T01:intron 50.0%
GGATGCAGCATTCCATGGTT+TGG - Chr8:74964079-74964098 MsG0880046568.01.T01:intron 50.0%
GGTAAGGATGCAGCATTCCA+TGG - Chr8:74964074-74964093 MsG0880046568.01.T01:intron 50.0%
GTGCCTTATACTGATGGCTG+AGG + Chr8:74963539-74963558 None:intergenic 50.0%
TCATCACCTTAAGTAGGCGC+CGG - Chr8:74963565-74963584 MsG0880046568.01.T01:intron 50.0%
TCCGTTGCTAGGTAGACAGA+TGG - Chr8:74964022-74964041 MsG0880046568.01.T01:intron 50.0%
TCTTCTTACCACGGCGTCTA+CGG + Chr8:74963256-74963275 None:intergenic 50.0%
TGGAGTGGACGAAACACTCA+AGG - Chr8:74964348-74964367 MsG0880046568.01.T01:intron 50.0%
!! ACTGATGGCTGAGGGTACTA+GGG + Chr8:74963530-74963549 None:intergenic 50.0%
!! TACTGATGGCTGAGGGTACT+AGG + Chr8:74963531-74963550 None:intergenic 50.0%
AAAAGATTGGGACCCACCCG+AGG + Chr8:74963664-74963683 None:intergenic 55.0%
ACTAAGTGGGGTCGGCTACA+TGG + Chr8:74963422-74963441 None:intergenic 55.0%
ATGTAGCCGACCCCACTTAG+TGG - Chr8:74963421-74963440 MsG0880046568.01.T01:CDS 55.0%
CATCACCTTAAGTAGGCGCC+GGG - Chr8:74963566-74963585 MsG0880046568.01.T01:intron 55.0%
CGACCCCACTTAGTGGGATA+AGG - Chr8:74963428-74963447 MsG0880046568.01.T01:CDS 55.0%
CTGATGGAGTTCAGCGCGAA+TGG - Chr8:74964395-74964414 MsG0880046568.01.T01:intron 55.0%
CTTCTTACCACGGCGTCTAC+GGG + Chr8:74963255-74963274 None:intergenic 55.0%
TGGAGTTCAGCGCGAATGGA+TGG - Chr8:74964399-74964418 MsG0880046568.01.T01:intron 55.0%
TGTAGCCGACCCCACTTAGT+GGG - Chr8:74963422-74963441 MsG0880046568.01.T01:CDS 55.0%
AGGTTGCACAGGGGAGGGTT+TGG - Chr8:74964048-74964067 MsG0880046568.01.T01:intron 60.0%
GGAAGAGGTTGCACAGGGGA+GGG - Chr8:74964043-74964062 MsG0880046568.01.T01:intron 60.0%
GTAAGCCCGGCGCCTACTTA+AGG + Chr8:74963574-74963593 None:intergenic 60.0%
TGGAAGAGGTTGCACAGGGG+AGG - Chr8:74964042-74964061 MsG0880046568.01.T01:intron 60.0%
GAGAAGACCCGTAGACGCCG+TGG - Chr8:74963245-74963264 MsG0880046568.01.T01:three_prime_UTR 65.0%
GCACAGGGGAGGGTTTGGAC+CGG - Chr8:74964053-74964072 MsG0880046568.01.T01:intron 65.0%
GGGGAGGGTTTGGACCGGTA+AGG - Chr8:74964058-74964077 MsG0880046568.01.T01:intron 65.0%
!! AGGCGCCGGGCTTACAGTGT+AGG - Chr8:74963579-74963598 MsG0880046568.01.T01:intron 65.0%
Chromosome Type Strat End Strand Name
Chr8 gene 74963108 74964749 74963108 ID=MsG0880046568.01;Name=MsG0880046568.01
Chr8 mRNA 74963108 74964749 74963108 ID=MsG0880046568.01.T01;Parent=MsG0880046568.01;Name=MsG0880046568.01.T01;_AED=0.62;_eAED=0.62;_QI=0|0.5|0.66|0.66|1|1|3|212|226
Chr8 exon 74963108 74963580 74963108 ID=MsG0880046568.01.T01:exon:31411;Parent=MsG0880046568.01.T01
Chr8 exon 74963684 74963824 74963684 ID=MsG0880046568.01.T01:exon:31410;Parent=MsG0880046568.01.T01
Chr8 exon 74964471 74964749 74964471 ID=MsG0880046568.01.T01:exon:31409;Parent=MsG0880046568.01.T01
Chr8 CDS 74964471 74964749 74964471 ID=MsG0880046568.01.T01:cds;Parent=MsG0880046568.01.T01
Chr8 CDS 74963684 74963824 74963684 ID=MsG0880046568.01.T01:cds;Parent=MsG0880046568.01.T01
Chr8 CDS 74963320 74963580 74963320 ID=MsG0880046568.01.T01:cds;Parent=MsG0880046568.01.T01
Chr8 three_prime_UTR 74963108 74963319 74963108 ID=MsG0880046568.01.T01:three_prime_utr;Parent=MsG0880046568.01.T01
Gene Sequence

>MsG0880046568.01.T01

ATGAGGATGTTGCGTTGGATGAGTGGTAAGACTAGACATGATAGGATTAGGAATGACATCATTAGAGAGAGAGTGGGGGTAGCACCTATAGTAGAAAAGTTGGTAGAAAATAGGCTTAGATGGTTTGGACATGTAGAGAGAAGACCCGTAGACGCCGTGGTAAGAAGAGTAGATCAAATGGAGGAGAGTCAGGTTAAAAGAGGTAGAGGAAGACCTAAGAAAACTATTAGAGAAACCATTAGAAAAGATTTAGAGGTCAATGAGTTGGATCCAAATATGGTAGACAGATGGAAGAGGTTGCACAGGGGAGGGTTTGGACCGGTAAGGATGCAGCATTCCATGGTTTGGTTGATGCTATTGGTGGGAATCTCGTGCTATTGCTATGCAAAATTGAAAGCTAATATACCTCAAGACAGACAGGTTACTGTTGTGGAGCTCTCTAGACCTCGTCCTCTTACCGAGATTTTAAGAGCGTCTGGTAATTCTCTGGTTGGAGTGGACGAAACACTCAAGGAACTACTAGAGGACTTGACATTTTCTGATGGAGTTCAGCGCGAATGGATGGAATCATGTTTCAGAAATTGGAAGGAAATCCATCTGCTAACCCATTTTGGCATTGATGAAAGATTACCTCAGTTCTACTACATAGAGAGGGGGGAGACTTTCTTCATTGTTGTATAA
Protein sequence

>MsG0880046568.01.T01

MRMLRWMSGKTRHDRIRNDIIRERVGVAPIVEKLVENRLRWFGHVERRPVDAVVRRVDQMEESQVKRGRGRPKKTIRETIRKDLEVNELDPNMVDRWKRLHRGGFGPVRMQHSMVWLMLLVGISCYCYAKLKANIPQDRQVTVVELSRPRPLTEILRASGNSLVGVDETLKELLEDLTFSDGVQREWMESCFRNWKEIHLLTHFGIDERLPQFYYIERGETFFIVV*