AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0880046648.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880046648.01.T01 MTR_4g111640 93.923 181 11 0 1 181 1 181 2.30e-127 355
MsG0880046648.01.T01 MTR_4g077787 85.475 179 24 2 1 178 1 178 5.57e-113 319
MsG0880046648.01.T01 MTR_4g112310 98.473 131 2 0 51 181 1 131 9.57e-95 271
MsG0880046648.01.T01 MTR_6g022170 55.833 120 50 2 23 140 25 143 3.27e-43 142
MsG0880046648.01.T01 MTR_2g101300 38.356 146 85 3 12 155 5 147 9.65e-33 115
MsG0880046648.01.T01 MTR_3g105930 41.722 151 78 5 31 173 26 174 1.30e-31 112
MsG0880046648.01.T01 MTR_4g078410 36.095 169 84 5 31 180 24 187 3.65e-30 109
MsG0880046648.01.T01 MTR_2g090580 37.838 148 79 4 13 151 8 151 9.09e-30 108
MsG0880046648.01.T01 MTR_2g079030 37.821 156 82 3 14 158 7 158 8.00e-28 108
MsG0880046648.01.T01 MTR_8g463180 41.304 138 72 3 11 146 6 136 2.22e-27 105
MsG0880046648.01.T01 MTR_8g095013 37.190 121 71 2 13 131 6 123 1.46e-25 100
MsG0880046648.01.T01 MTR_8g086360 35.811 148 85 4 8 153 3 142 1.87e-25 97.4
MsG0880046648.01.T01 MTR_1g014120 36.424 151 76 6 7 146 4 145 4.40e-25 96.3
MsG0880046648.01.T01 MTR_8g094990 36.364 121 72 2 13 131 6 123 8.84e-24 95.1
MsG0880046648.01.T01 MTR_2g090575 37.398 123 68 3 12 132 8 123 2.35e-23 95.1
MsG0880046648.01.T01 MTR_7g090170 37.226 137 64 6 29 160 24 143 7.68e-21 84.3
MsG0880046648.01.T01 MTR_4g124280 36.735 147 68 5 32 157 25 167 1.00e-20 85.9
MsG0880046648.01.T01 MTR_8g095020 35.246 122 72 3 13 132 6 122 1.24e-20 87.0
MsG0880046648.01.T01 MTR_1g077790 37.143 105 63 2 29 131 26 129 1.60e-20 84.3
MsG0880046648.01.T01 MTR_4g081100 34.426 122 72 3 13 132 17 132 2.74e-20 84.7
MsG0880046648.01.T01 MTR_2g083250 38.095 105 61 3 31 132 25 128 2.12e-19 82.8
MsG0880046648.01.T01 MTR_4g114870 37.255 102 60 2 32 131 191 290 2.89e-18 81.3
MsG0880046648.01.T01 MTR_4g114870 31.667 120 77 3 30 146 27 144 5.09e-15 72.0
MsG0880046648.01.T01 MTR_5g006040 30.909 165 100 6 26 181 20 179 4.16e-18 77.8
MsG0880046648.01.T01 MTR_0334s0010 35.294 102 62 2 31 131 27 125 6.87e-17 75.1
MsG0880046648.01.T01 MTR_1g090420 31.250 144 93 3 11 151 7 147 1.14e-16 75.1
MsG0880046648.01.T01 MTR_4g067200 30.709 127 82 3 14 136 10 134 1.53e-16 73.6
MsG0880046648.01.T01 MTR_4g066110 30.709 127 82 3 14 136 10 134 1.53e-16 73.6
MsG0880046648.01.T01 MTR_6g083240 32.000 150 89 5 32 173 27 171 7.95e-16 71.6
MsG0880046648.01.T01 MTR_1g104800 34.906 106 63 2 32 135 25 126 1.40e-15 71.2
MsG0880046648.01.T01 MTR_3g092170 28.834 163 88 3 13 151 6 164 1.01e-14 70.5
MsG0880046648.01.T01 MTR_2g088990 32.174 115 70 3 29 141 22 130 1.62e-14 69.3
MsG0880046648.01.T01 MTR_3g099570 31.690 142 84 6 14 151 7 139 4.10e-14 67.4
MsG0880046648.01.T01 MTR_6g023760 36.538 104 58 3 30 131 24 121 1.37e-13 65.9
MsG0880046648.01.T01 MTR_3g099580 34.127 126 71 4 9 131 4 120 1.60e-13 65.9
MsG0880046648.01.T01 MTR_6g013420 28.859 149 93 5 4 146 8 149 2.56e-13 65.1
MsG0880046648.01.T01 MTR_4g130780 33.588 131 76 6 7 131 4 129 6.97e-12 61.2
MsG0880046648.01.T01 MTR_2g025580 27.811 169 110 5 14 176 7 169 8.85e-12 60.8
MsG0880046648.01.T01 MTR_3g099540 32.039 103 63 3 30 131 25 121 9.41e-12 60.1
MsG0880046648.01.T01 MTR_4g114830 30.952 126 80 5 26 146 22 145 1.49e-11 60.1
MsG0880046648.01.T01 MTR_3g099980 32.258 124 74 4 14 133 7 124 9.96e-11 58.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880046648.01.T01 AT1G48940 48.851 174 71 4 11 173 10 176 3.28e-52 165
MsG0880046648.01.T01 AT3G18590 64.151 106 38 0 27 132 22 127 7.96e-50 159
MsG0880046648.01.T01 AT5G14345 43.704 135 70 2 12 146 1 129 9.87e-36 122
MsG0880046648.01.T01 AT1G79800 41.129 124 72 1 12 135 14 136 5.22e-31 111
MsG0880046648.01.T01 AT4G28365 38.194 144 78 3 12 148 9 148 1.61e-30 110
MsG0880046648.01.T01 AT4G31840 40.559 143 77 4 12 151 10 147 8.91e-30 108
MsG0880046648.01.T01 AT5G53870 35.632 174 92 5 1 164 1 164 4.06e-28 108
MsG0880046648.01.T01 AT5G53870 35.632 174 92 5 1 164 1 164 5.46e-28 107
MsG0880046648.01.T01 AT4G32490 36.429 140 83 2 28 165 27 162 2.22e-26 100
MsG0880046648.01.T01 AT2G25060 37.857 140 76 4 31 167 30 161 4.07e-25 96.3
MsG0880046648.01.T01 AT5G57920 43.363 113 57 4 25 132 13 123 3.62e-23 91.3
MsG0880046648.01.T01 AT4G30590 38.710 124 71 3 12 132 8 129 8.37e-23 90.5
MsG0880046648.01.T01 AT5G57920 43.810 105 54 3 31 132 22 124 9.47e-23 90.1
MsG0880046648.01.T01 AT5G57920 41.176 119 62 4 31 143 54 170 1.99e-22 90.1
MsG0880046648.01.T01 AT5G25090 41.667 120 64 4 17 133 12 128 7.45e-22 87.8
MsG0880046648.01.T01 AT4G27520 31.944 144 92 2 5 146 4 143 4.99e-21 88.6
MsG0880046648.01.T01 AT1G64640 30.216 139 92 2 12 148 13 148 3.34e-20 83.6
MsG0880046648.01.T01 AT3G20570 36.538 104 61 2 31 132 29 129 3.59e-20 84.0
MsG0880046648.01.T01 AT2G31050 33.571 140 84 3 3 140 2 134 4.38e-19 80.9
MsG0880046648.01.T01 AT2G26720 36.885 122 70 3 13 132 10 126 7.28e-19 80.5
MsG0880046648.01.T01 AT2G23990 34.483 145 81 7 12 151 11 146 1.72e-18 79.7
MsG0880046648.01.T01 AT2G32300 33.071 127 75 4 29 151 23 143 1.04e-17 78.6
MsG0880046648.01.T01 AT2G32300 33.333 126 74 4 30 151 54 173 1.69e-17 78.6
MsG0880046648.01.T01 AT3G60270 33.333 102 61 3 32 132 26 121 1.69e-14 68.6
MsG0880046648.01.T01 AT5G26330 29.851 134 80 4 1 132 1 122 2.06e-14 68.2
MsG0880046648.01.T01 AT2G23990 30.488 164 81 8 12 151 11 165 4.32e-14 68.2
MsG0880046648.01.T01 AT5G07475 30.657 137 87 3 12 146 10 140 9.98e-14 66.6
MsG0880046648.01.T01 AT2G27035 30.631 111 66 5 35 142 32 134 1.78e-11 60.1

Find 40 sgRNAs with CRISPR-Local

Find 52 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTGAAGTTGGTGGTAAAAT+TGG 0.248593 8:-76124425 MsG0880046648.01.T01:CDS
TTTCTTTGCCTCCCAATCTT+TGG 0.264284 8:-76124465 MsG0880046648.01.T01:CDS
TCATCACCATCCTTTGAGTT+AGG 0.279895 8:+76124392 None:intergenic
GGTGAGGGTGCTGTTGGTTC+AGG 0.348949 8:+76123903 None:intergenic
CTATTTGCTTTGTCATTCCT+TGG 0.356691 8:-76123801 MsG0880046648.01.T01:CDS
TACTTCATTAGTGGTGTTAG+TGG 0.359912 8:-76123975 MsG0880046648.01.T01:CDS
GTGAATGCTGTGATAGTAAT+AGG 0.400127 8:+76123825 None:intergenic
TACAATTGATGACCAAAGAT+TGG 0.408666 8:+76124453 None:intergenic
CATCACCATCCTTTGAGTTA+GGG 0.425498 8:+76124393 None:intergenic
GGATTGTTCTACTTCATTAG+TGG 0.430222 8:-76123984 MsG0880046648.01.T01:CDS
GTGATGAAATGTACAACAAA+TGG 0.453312 8:-76124376 MsG0880046648.01.T01:CDS
GCAGCTTTAGAATGTGCTAT+TGG 0.459227 8:+76123858 None:intergenic
TCGACTCGTCCACTCTTCTT+CGG 0.480946 8:-76124044 MsG0880046648.01.T01:CDS
GCAAAGGAAAAGTGGGTATG+AGG 0.483957 8:+76124496 None:intergenic
AGTTGGTGGTAAAATTGGTT+GGG 0.488516 8:-76124420 MsG0880046648.01.T01:CDS
GCATTCTCATTTGCAGATTG+TGG 0.492826 8:+76123882 None:intergenic
ACAATTGATGACCAAAGATT+GGG 0.505414 8:+76124454 None:intergenic
GATTGTGGTGAGGGTGCTGT+TGG 0.512152 8:+76123897 None:intergenic
AATTGTACTGAATTTGAAGT+TGG 0.513642 8:-76124437 MsG0880046648.01.T01:CDS
TCATTTGCAGATTGTGGTGA+GGG 0.517209 8:+76123888 None:intergenic
CCATTGTTTCCGAAGAAGAG+TGG 0.518696 8:+76124035 None:intergenic
CTCATTTGCAGATTGTGGTG+AGG 0.521847 8:+76123887 None:intergenic
ATAATCAAGCAAAGGAAAAG+TGG 0.531969 8:+76124488 None:intergenic
TGTACTGAATTTGAAGTTGG+TGG 0.533474 8:-76124434 MsG0880046648.01.T01:CDS
CTAATGAAGTAGAACAATCC+TGG 0.545709 8:+76123987 None:intergenic
AAGTTGGTGGTAAAATTGGT+TGG 0.549766 8:-76124421 MsG0880046648.01.T01:CDS
TGATGAAATGTACAACAAAT+GGG 0.552171 8:-76124375 MsG0880046648.01.T01:CDS
TAATCAAGCAAAGGAAAAGT+GGG 0.571116 8:+76124489 None:intergenic
CCACTCTTCTTCGGAAACAA+TGG 0.572032 8:-76124035 MsG0880046648.01.T01:CDS
CGAGAAAGATTCAGTGATGG+TGG 0.591369 8:-76124096 MsG0880046648.01.T01:CDS
CTTCGGAAACAATGGCAACA+CGG 0.597439 8:-76124027 MsG0880046648.01.T01:CDS
TGGTGGTAAAATTGGTTGGG+TGG 0.599284 8:-76124417 MsG0880046648.01.T01:CDS
GTTAGTGGTCATTGTACAAG+AGG 0.601760 8:-76123960 MsG0880046648.01.T01:CDS
CAAAGAAAATAATCAAGCAA+AGG 0.602288 8:+76124480 None:intergenic
TTGGGTGGTCCCTAACTCAA+AGG 0.612930 8:-76124402 MsG0880046648.01.T01:CDS
GTATGAGGAAGCTTTGAAGA+TGG 0.616797 8:+76124511 None:intergenic
TTCAAGCATAAATCATGCCA+AGG 0.629433 8:+76123784 None:intergenic
GTGGTCCCTAACTCAAAGGA+TGG 0.629713 8:-76124398 MsG0880046648.01.T01:CDS
ATTGATGACCAAAGATTGGG+AGG 0.657515 8:+76124457 None:intergenic
GTACGAGAAAGATTCAGTGA+TGG 0.658831 8:-76124099 MsG0880046648.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATGTATCTTAAAATTAAAT+CGG + Chr8:76124007-76124026 None:intergenic 10.0%
!! CAAAAGTTATATAACATAAA+TGG + Chr8:76124159-76124178 None:intergenic 15.0%
!!! TTTCTAAAAAGAAATGAATA+AGG - Chr8:76124061-76124080 MsG0880046648.01.T01:CDS 15.0%
!!! ATCAATTTTGAATCTGTTTT+GGG + Chr8:76123957-76123976 None:intergenic 20.0%
!!! TAACTTTTGTTTTGAACTTT+TGG - Chr8:76124170-76124189 MsG0880046648.01.T01:intron 20.0%
! AAAATTAAATCGGTAAGAAG+TGG + Chr8:76123997-76124016 None:intergenic 25.0%
! CAAAGAAAATAATCAAGCAA+AGG + Chr8:76123826-76123845 None:intergenic 25.0%
! TGATGAAATGTACAACAAAT+GGG - Chr8:76123928-76123947 MsG0880046648.01.T01:CDS 25.0%
!! AAAAATGATCATCAAGGTTT+TGG - Chr8:76124369-76124388 MsG0880046648.01.T01:CDS 25.0%
!! AATTGTACTGAATTTGAAGT+TGG - Chr8:76123866-76123885 MsG0880046648.01.T01:CDS 25.0%
!! CATCAATTTTGAATCTGTTT+TGG + Chr8:76123958-76123977 None:intergenic 25.0%
AGGACAAAAAATGATCATCA+AGG - Chr8:76124363-76124382 MsG0880046648.01.T01:CDS 30.0%
ATAATCAAGCAAAGGAAAAG+TGG + Chr8:76123818-76123837 None:intergenic 30.0%
GTGATGAAATGTACAACAAA+TGG - Chr8:76123927-76123946 MsG0880046648.01.T01:CDS 30.0%
TAATCAAGCAAAGGAAAAGT+GGG + Chr8:76123817-76123836 None:intergenic 30.0%
! ACAATTGATGACCAAAGATT+GGG + Chr8:76123852-76123871 None:intergenic 30.0%
! TACAATTGATGACCAAAGAT+TGG + Chr8:76123853-76123872 None:intergenic 30.0%
!! TTTGAAGTTGGTGGTAAAAT+TGG - Chr8:76123878-76123897 MsG0880046648.01.T01:CDS 30.0%
CTAATGAAGTAGAACAATCC+TGG + Chr8:76124319-76124338 None:intergenic 35.0%
CTATTTGCTTTGTCATTCCT+TGG - Chr8:76124502-76124521 MsG0880046648.01.T01:CDS 35.0%
GGATTGTTCTACTTCATTAG+TGG - Chr8:76124319-76124338 MsG0880046648.01.T01:intron 35.0%
GTGAATGCTGTGATAGTAAT+AGG + Chr8:76124481-76124500 None:intergenic 35.0%
TAAGGTCACTACAAAAGTGA+AGG - Chr8:76124079-76124098 MsG0880046648.01.T01:CDS 35.0%
! GTTTTCAAATTTGAACGACC+AGG - Chr8:76124298-76124317 MsG0880046648.01.T01:intron 35.0%
! TACTTCATTAGTGGTGTTAG+TGG - Chr8:76124328-76124347 MsG0880046648.01.T01:intron 35.0%
! TGTACTGAATTTGAAGTTGG+TGG - Chr8:76123869-76123888 MsG0880046648.01.T01:CDS 35.0%
!! AAGTTGGTGGTAAAATTGGT+TGG - Chr8:76123882-76123901 MsG0880046648.01.T01:CDS 35.0%
!! AGTTGGTGGTAAAATTGGTT+GGG - Chr8:76123883-76123902 MsG0880046648.01.T01:CDS 35.0%
GCATTCTCATTTGCAGATTG+TGG + Chr8:76124424-76124443 None:intergenic 40.0%
GTACGAGAAAGATTCAGTGA+TGG - Chr8:76124204-76124223 MsG0880046648.01.T01:intron 40.0%
GTTAGTGGTCATTGTACAAG+AGG - Chr8:76124343-76124362 MsG0880046648.01.T01:CDS 40.0%
TCATTTGCAGATTGTGGTGA+GGG + Chr8:76124418-76124437 None:intergenic 40.0%
TTTCTTTGCCTCCCAATCTT+TGG - Chr8:76123838-76123857 MsG0880046648.01.T01:CDS 40.0%
! ATTGATGACCAAAGATTGGG+AGG + Chr8:76123849-76123868 None:intergenic 40.0%
! CATCACCATCCTTTGAGTTA+GGG + Chr8:76123913-76123932 None:intergenic 40.0%
! TCATCACCATCCTTTGAGTT+AGG + Chr8:76123914-76123933 None:intergenic 40.0%
!! GCAGCTTTAGAATGTGCTAT+TGG + Chr8:76124448-76124467 None:intergenic 40.0%
!! GTATGAGGAAGCTTTGAAGA+TGG + Chr8:76123795-76123814 None:intergenic 40.0%
CCACTCTTCTTCGGAAACAA+TGG - Chr8:76124268-76124287 MsG0880046648.01.T01:intron 45.0%
CCATTGTTTCCGAAGAAGAG+TGG + Chr8:76124271-76124290 None:intergenic 45.0%
CGAGAAAGATTCAGTGATGG+TGG - Chr8:76124207-76124226 MsG0880046648.01.T01:intron 45.0%
CTCATTTGCAGATTGTGGTG+AGG + Chr8:76124419-76124438 None:intergenic 45.0%
CTTCGGAAACAATGGCAACA+CGG - Chr8:76124276-76124295 MsG0880046648.01.T01:intron 45.0%
GCAAAGGAAAAGTGGGTATG+AGG + Chr8:76123810-76123829 None:intergenic 45.0%
TGGTGGTAAAATTGGTTGGG+TGG - Chr8:76123886-76123905 MsG0880046648.01.T01:CDS 45.0%
!!! ATGATATATTTTATTTTTAA+AGG - Chr8:76124111-76124130 MsG0880046648.01.T01:intron 5.0%
!!! TGATATATTTTATTTTTAAA+GGG - Chr8:76124112-76124131 MsG0880046648.01.T01:intron 5.0%
GTGGTCCCTAACTCAAAGGA+TGG - Chr8:76123905-76123924 MsG0880046648.01.T01:CDS 50.0%
TTGGGTGGTCCCTAACTCAA+AGG - Chr8:76123901-76123920 MsG0880046648.01.T01:CDS 50.0%
! TCGACTCGTCCACTCTTCTT+CGG - Chr8:76124259-76124278 MsG0880046648.01.T01:intron 50.0%
!! GATTGTGGTGAGGGTGCTGT+TGG + Chr8:76124409-76124428 None:intergenic 55.0%
!! GGTGAGGGTGCTGTTGGTTC+AGG + Chr8:76124403-76124422 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
Chr8 gene 76123785 76124540 76123785 ID=MsG0880046648.01;Name=MsG0880046648.01
Chr8 mRNA 76123785 76124540 76123785 ID=MsG0880046648.01.T01;Parent=MsG0880046648.01;Name=MsG0880046648.01.T01;_AED=0.02;_eAED=0.02;_QI=0|0|0|1|1|1|2|0|181
Chr8 exon 76124339 76124540 76124339 ID=MsG0880046648.01.T01:exon:8348;Parent=MsG0880046648.01.T01
Chr8 exon 76123785 76124128 76123785 ID=MsG0880046648.01.T01:exon:8347;Parent=MsG0880046648.01.T01
Chr8 CDS 76124339 76124540 76124339 ID=MsG0880046648.01.T01:cds;Parent=MsG0880046648.01.T01
Chr8 CDS 76123785 76124128 76123785 ID=MsG0880046648.01.T01:cds;Parent=MsG0880046648.01.T01
Gene Sequence

>MsG0880046648.01.T01

ATGGCTGCCATCTTCAAAGCTTCCTCATACCCACTTTTCCTTTGCTTGATTATTTTCTTTGCCTCCCAATCTTTGGTCATCAATTGTACTGAATTTGAAGTTGGTGGTAAAATTGGTTGGGTGGTCCCTAACTCAAAGGATGGTGATGAAATGTACAACAAATGGGCTTCCCAAAACAGATTCAAAATTGATGATATTATTCATTTCAAGTACGAGAAAGATTCAGTGATGGTGGTTAGTGAAGAAGAATATGAGAATTGCAAATCGACTCGTCCACTCTTCTTCGGAAACAATGGCAACACGGTTTTCAAATTTGAACGACCAGGATTGTTCTACTTCATTAGTGGTGTTAGTGGTCATTGTACAAGAGGACAAAAAATGATCATCAAGGTTTTGGATGTTGAACCTGAACCAACAGCACCCTCACCACAATCTGCAAATGAGAATGCACCAATAGCACATTCTAAAGCTGCTCAAATAACTCCTATTACTATCACAGCATTCACACTATTTGCTTTGTCATTCCTTGGCATGATTTATGCTTGA

Protein sequence

>MsG0880046648.01.T01

MAAIFKASSYPLFLCLIIFFASQSLVINCTEFEVGGKIGWVVPNSKDGDEMYNKWASQNRFKIDDIIHFKYEKDSVMVVSEEEYENCKSTRPLFFGNNGNTVFKFERPGLFYFISGVSGHCTRGQKMIIKVLDVEPEPTAPSPQSANENAPIAHSKAAQITPITITAFTLFALSFLGMIYA*